CRISPOR

DNA break repair outcome predictions

Guide: GGCCTCCCCCGCCACGCTCC CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`ATCGACCGGCTCCTCCTCCTGGCCTCCCCCGCCACGCTCCCGGGAAGTCGGAGGGCCGGCTCGCGGGCTCAGGCAACCGT`	(Wild-type sequence)
296	`TCCTCCTCCTGGCCTCCCCCGCCACG------GGAAGTCGGAGGGCCGGCTCGCGGGCTC`	`6 bp deleted → no frameshift`
230	`TCCTCCTCCTGGCCTCCCCCG-----------GGAAGTCGGAGGGCCGGCTCGCGGGCTC`	`11 bp deleted → frameshift`
221	`TCCTCCTCCTGGCCTCCCCCGCCACGCTC-----------------------GCGGGCTC`	`23 bp deleted → frameshift`
198	`TCCTCCTCCTGGCCTCCCCCGCCACG--------------GAGGGCCGGCTCGCGGGCTC`	`14 bp deleted → frameshift`
182	`TCCTCCTCCTGGCCTCCCCCGCCACGCTC----------GGAGGGCCGGCTCGCGGGCTC`	`10 bp deleted → frameshift`
180	`TCCTCCTCCTGGCCTCCCCCGCC------------------------GGCTCGCGGGCTC`	`24 bp deleted → no frameshift`
170	`TCCTCCTCCTGGCCTCCCCCGCCACGCTCC-----------------GGCTCGCGGGCTC`	`17 bp deleted → frameshift`
155	`TCCTCCTCCTGGCCTCCCCCGCCACGC---------------------------GGGCTC`	`27 bp deleted → no frameshift`
155	`TCCTCCTCCTGGCCTCCCCCG---------------------------GCTCGCGGGCTC`	`27 bp deleted → no frameshift`
154	`TCCTCCTCCTGGCCTCCCCCGCCACGC-------------------CGGCTCGCGGGCTC`	`19 bp deleted → frameshift`
154	`TCCTCCTCCTGGCCTCCCCCG-------------------GAGGGCCGGCTCGCGGGCTC`	`19 bp deleted → frameshift`
153	`TCCTCCTCCTGGCC---------------------------------GGCTCGCGGGCTC`	`33 bp deleted → no frameshift`
148	`TCCTCCTCCTGGCCTCCCCCGCCACGCTC-------------------------------`	`31 bp deleted → frameshift`
140	`TCCTCCTCCTGG---------------------GAAGTCGGAGGGCCGGCTCGCGGGCTC`	`21 bp deleted → no frameshift`
133	`TCCTCCTCCTGGCCTCCCCCGCCACG----------------------GCTCGCGGGCTC`	`22 bp deleted → frameshift`
133	`TCCTCCTCCTGG----------------------AAGTCGGAGGGCCGGCTCGCGGGCTC`	`22 bp deleted → frameshift`
121	`TCCTCCTCCTGGCCTCCCCCGC--------------------------------GGGCTC`	`32 bp deleted → frameshift`
98	`TCCTCCTCCTGGCCTCCCCCGC----------------------------TCGCGGGCTC`	`28 bp deleted → frameshift`
98	`TCCTCCTCCTGGCCTCCCC----------------------------GGCTCGCGGGCTC`	`28 bp deleted → frameshift`
95	`TCCTCCTCCTGGCCTC-----------------------GGAGGGCCGGCTCGCGGGCTC`	`23 bp deleted → frameshift`
94	`TCCTCCTCCTGGC-------------------------------------TCGCGGGCTC`	`37 bp deleted → frameshift`
94	`TCCTCCTCCTGGCCTCCCCCGCCACG-----------------------------GGCTC`	`29 bp deleted → frameshift`
94	`TCCTCCTCCTGGCCTCCC-----------------------------GGCTCGCGGGCTC`	`29 bp deleted → frameshift`
94	`TCCTCCTCCTGG-----------------------------AGGGCCGGCTCGCGGGCTC`	`29 bp deleted → frameshift`
89	`TCCTCCTCCTGGCCTCC------------------------------GGCTCGCGGGCTC`	`30 bp deleted → no frameshift`
82	`TCCTCCTCCTGGCCTC------------------------------------GCGGGCTC`	`36 bp deleted → no frameshift`
80	`TCCTCCTCCTGG--------------------------------GCCGGCTCGCGGGCTC`	`32 bp deleted → frameshift`
73	`TCCTCCTCCTGGCCTCCCCCG----------------------------------GGCTC`	`34 bp deleted → frameshift`
66	`TCCTCCTCCTGGCCTCCCCCGC------------------------------------TC`	`36 bp deleted → no frameshift`
63	`TCCTCCTC-------------------------------GGAGGGCCGGCTCGCGGGCTC`	`31 bp deleted → frameshift`
63	`TCCTCCTCCTGGC---------------------------------------------TC`	`45 bp deleted → no frameshift`
60	`TCCTCCTCC--------------------------------------GGCTCGCGGGCTC`	`38 bp deleted → frameshift`
55	`TCCTCCTCCTGGCCTC--------------------------------------------`	`44 bp deleted → frameshift`
55	`TCCTCCTC--------------------------------------------GCGGGCTC`	`44 bp deleted → frameshift`
54	`TCCTC----------------------------------GGAGGGCCGGCTCGCGGGCTC`	`34 bp deleted → frameshift`
51	`TCCTCCTCCTGGC-----------------------------------------GGGCTC`	`41 bp deleted → frameshift`
51	`TCCTCC-----------------------------------------GGCTCGCGGGCTC`	`41 bp deleted → frameshift`
47	`TCCTC-----------------------------------------------GCGGGCTC`	`47 bp deleted → frameshift`
47	`TC-------------------------------------GGAGGGCCGGCTCGCGGGCTC`	`37 bp deleted → frameshift`
44	`TCCTCCTCCTGG--------------------------------------------GCTC`	`44 bp deleted → frameshift`
44	`TCC--------------------------------------------GGCTCGCGGGCTC`	`44 bp deleted → frameshift`
38	`TCCTCCTCCT-----------------------------------------CGCGGGCTC`	`41 bp deleted → frameshift`
37	`TCCTCCTC----------------------------------------------------`	`52 bp deleted → frameshift`
32	`TCCTC-------------------------------------------------------`	`55 bp deleted → frameshift`
25	`TCCTCCTCCT-------------------------------------------------C`	`49 bp deleted → frameshift`
24	`TC--------------------------------------------------GCGGGCTC`	`50 bp deleted → frameshift`
16	`TC----------------------------------------------------------`	`58 bp deleted → frameshift`

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