CRISPOR

DNA break repair outcome predictions

Guide: AAGGCGACAAGGCAGGCGCC GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CTTGGCTGAGAAGAGGAGCTAAGGCGACAAGGCAGGCGCCGGGTAGCCCCAGCGCCGTGTCGACGGCGACCGCGGAGATG`	(Wild-type sequence)
449	`AAGAGGAGCTAAGGCGACAAGGCAGGCGCC----------------GTGTCGACGGCGAC`	`16 bp deleted → frameshift`
402	`AAGAGGAGCTAAGGCGACAAGGCAGGCGCC--------CCAGCGCCGTGTCGACGGCGAC`	`8 bp deleted → frameshift`
296	`AAGAGGAGCTAAGGCGACAAGGCAGG------GTAGCCCCAGCGCCGTGTCGACGGCGAC`	`6 bp deleted → no frameshift`
282	`AAGAGGAGCTAAGGCGACAAGGCAGG-------TAGCCCCAGCGCCGTGTCGACGGCGAC`	`7 bp deleted → frameshift`
255	`AAGAGGAGCTAAGGCGACAAGGCAGGCGCC---------CAGCGCCGTGTCGACGGCGAC`	`9 bp deleted → no frameshift`
242	`AAGAGGAGCTAAGGCGACAAGGCAGGCGCC----------AGCGCCGTGTCGACGGCGAC`	`10 bp deleted → frameshift`
242	`AAGAGGAGCTAAGGCGACAAGGCAGGC----------CCCAGCGCCGTGTCGACGGCGAC`	`10 bp deleted → frameshift`
242	`AAGAGGAGCTAAGGCGACAAGG----------GTAGCCCCAGCGCCGTGTCGACGGCGAC`	`10 bp deleted → frameshift`
230	`AAGAGGAGCTAAGGCGACAAGG-----------TAGCCCCAGCGCCGTGTCGACGGCGAC`	`11 bp deleted → frameshift`
213	`AAGAGGAGCTAAGGCGACAAGGCAG-----------------CGCCGTGTCGACGGCGAC`	`17 bp deleted → frameshift`
198	`AAGAGGAGCTAAGGCGACAAGGCAGGCGC--------------GCCGTGTCGACGGCGAC`	`14 bp deleted → frameshift`
198	`AAGAGGAGCTAAGGCGACAAGGC--------------CCCAGCGCCGTGTCGACGGCGAC`	`14 bp deleted → frameshift`
178	`AAGAGGAGCTAAGGCGACAAGGCAGGCG------------------------------AC`	`30 bp deleted → no frameshift`
173	`AAGAGGAGCTAAGGCGACAAGGCAG-----------CCCCAGCGCCGTGTCGACGGCGAC`	`11 bp deleted → frameshift`
162	`AAGAGGAGCTAAGGCGACAAGGCAGGC------------------CGTGTCGACGGCGAC`	`18 bp deleted → no frameshift`
162	`AAGAGGAGCTAAGG------------------GTAGCCCCAGCGCCGTGTCGACGGCGAC`	`18 bp deleted → no frameshift`
154	`AAGAGGAGCTAAGGCGACAAGGCAGGCG-------------------TGTCGACGGCGAC`	`19 bp deleted → frameshift`
154	`AAGAGGAGCTAAGG-------------------TAGCCCCAGCGCCGTGTCGACGGCGAC`	`19 bp deleted → frameshift`
148	`AAGAGGAGCTAAGGCG----------------------------CCGTGTCGACGGCGAC`	`28 bp deleted → frameshift`
147	`AAGAGGAGCTAAGGCGACAAGGC--------------------GCCGTGTCGACGGCGAC`	`20 bp deleted → frameshift`
141	`AAGAGGAGCTAAGGCGACAAG---------------CCCCAGCGCCGTGTCGACGGCGAC`	`15 bp deleted → no frameshift`
134	`AAGAGGAGCTAAGGCGAC------------------------------------------`	`42 bp deleted → no frameshift`
133	`AAGAGGAGCTAAGGCGACAAGGC----------------------CGTGTCGACGGCGAC`	`22 bp deleted → frameshift`
133	`AAGAGGAGCTAAGGC----------------------CCCAGCGCCGTGTCGACGGCGAC`	`22 bp deleted → frameshift`
123	`AAGAGGAGC----------------------------CCCAGCGCCGTGTCGACGGCGAC`	`28 bp deleted → frameshift`
120	`AAGAGGAGCTAAGGCGACAAGGCAGGCG------------------------ACGGCGAC`	`24 bp deleted → no frameshift`
115	`AAGAGGAGCTAAGGCGAC------------------------------------GGCGAC`	`36 bp deleted → no frameshift`
109	`AAGAGGAGCTAAGGCGACAAGGC----------------------------------GAC`	`34 bp deleted → frameshift`
109	`AAGAGG--------------------------GTAGCCCCAGCGCCGTGTCGACGGCGAC`	`26 bp deleted → frameshift`
105	`AAGAGGAGCTAAGGCGACAAG---------------------CGCCGTGTCGACGGCGAC`	`21 bp deleted → no frameshift`
103	`AAGAGGAGCTAAGGCGACAAGGCAGGCG---------------------------GCGAC`	`27 bp deleted → no frameshift`
103	`AAGAGG---------------------------TAGCCCCAGCGCCGTGTCGACGGCGAC`	`27 bp deleted → no frameshift`
99	`AAGAGGAGCTAAGGCGACA----------------------GCGCCGTGTCGACGGCGAC`	`22 bp deleted → frameshift`
98	`AAGAGGAGCTAAGGCGACAAGGCAGGCGC----------------------------GAC`	`28 bp deleted → frameshift`
95	`AAGAGGAGCTAAG-----------------------CCCCAGCGCCGTGTCGACGGCGAC`	`23 bp deleted → frameshift`
91	`AAGAGGAGC----------------------------------GCCGTGTCGACGGCGAC`	`34 bp deleted → frameshift`
89	`AAGAGGAGCTAAGGC------------------------------CGTGTCGACGGCGAC`	`30 bp deleted → no frameshift`
84	`AAGAGGAGCTAAGGCG-------------------------------TGTCGACGGCGAC`	`31 bp deleted → frameshift`
70	`AAGAGGAGCTAAG-----------------------------CGCCGTGTCGACGGCGAC`	`29 bp deleted → frameshift`
66	`AAGAGGAGC------------------------------------CGTGTCGACGGCGAC`	`36 bp deleted → no frameshift`
63	`AAGAG-------------------------------CCCCAGCGCCGTGTCGACGGCGAC`	`31 bp deleted → frameshift`
60	`AAGAGGAGCTA------------------------GCCCCAGCGCCGTGTCGACGGCGAC`	`24 bp deleted → no frameshift`
57	`AAG---------------------------------CCCCAGCGCCGTGTCGACGGCGAC`	`33 bp deleted → no frameshift`
56	`AAGAGGAGCTAAGGCG---------------------------------------GCGAC`	`39 bp deleted → no frameshift`
47	`AAGAG-------------------------------------CGCCGTGTCGACGGCGAC`	`37 bp deleted → frameshift`
42	`AAG---------------------------------------CGCCGTGTCGACGGCGAC`	`39 bp deleted → no frameshift`
36	`AAGAGGAGC------------------------------------------------GAC`	`48 bp deleted → no frameshift`
32	`AAGAGG--------------------------------------------------CGAC`	`50 bp deleted → frameshift`
30	`AAGAGGA----------------------------------------------CGGCGAC`	`46 bp deleted → frameshift`
25	`AAGA-------------------------------------------------CGGCGAC`	`49 bp deleted → frameshift`
22	`AAGAGGA----------------------------------------------------C`	`52 bp deleted → frameshift`
19	`AAGA-------------------------------------------------------C`	`55 bp deleted → frameshift`

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