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Contents:

PCR primers for off-targets of ATGGTTATGTTTCTGGATGG GGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313862_F Primer3: not found at this Tm N.d. 1.00
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313862_R Primer3: not found at this Tm N.d. 1.00
mm3_intergenic_Vdac3-ps1|Pou6f2_chr13_18078860_F Primer3: not found at this Tm N.d. 0.52
mm3_intergenic_Vdac3-ps1|Pou6f2_chr13_18078860_R Primer3: not found at this Tm N.d. 0.52
mm4_intergenic_Rpl21-ps10|Gm25574_chr3_38264254_F Primer3: not found at this Tm N.d. 0.48
mm4_intergenic_Rpl21-ps10|Gm25574_chr3_38264254_R Primer3: not found at this Tm N.d. 0.48
mm3_intergenic_D730003I15Rik|Nenf_chr1_191241422_F TCGTCGGCAGCGTCTGCCTGCTCTGACTTGGTAG 59.3 0.43
mm3_intergenic_D730003I15Rik|Nenf_chr1_191241422_R GTCTCGTGGGCTCGGTGCTGGAGTACCTACGACTGA 59.9 0.43
mm3_intergenic_C1galt1c1|Ap3s1-ps1_chrX_38673917_F TCGTCGGCAGCGTCGAAGATGCTCAGAGCCTCCC 59.8 0.42
mm3_intergenic_C1galt1c1|Ap3s1-ps1_chrX_38673917_R GTCTCGTGGGCTCGGACACGGGGTCATCATTCCAC 60.0 0.42
mm4_intergenic_Gm8793|Nkain2_chr10_31909870_F Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Gm8793|Nkain2_chr10_31909870_R Primer3: not found at this Tm N.d. 0.40
mm3_intergenic_Nr5a2|Gm23763_chr1_136969416_F TCGTCGGCAGCGTCGGTTCCCTTGTAGGCTGTTCA 59.9 0.37
mm3_intergenic_Nr5a2|Gm23763_chr1_136969416_R GTCTCGTGGGCTCGGAAACCCACTGGAAGGCACTC 60.1 0.37
mm4_intron_Dclk3_chr9_111451034_F Primer3: not found at this Tm N.d. 0.35
mm4_intron_Dclk3_chr9_111451034_R Primer3: not found at this Tm N.d. 0.35
mm3_intergenic_Fmr1|Fmr1nb_chrX_68731057_F Primer3: not found at this Tm N.d. 0.34
mm3_intergenic_Fmr1|Fmr1nb_chrX_68731057_R Primer3: not found at this Tm N.d. 0.34
mm4_intergenic_Gm23752|Gm24815_chr12_7234513_F Primer3: not found at this Tm N.d. 0.33
mm4_intergenic_Gm23752|Gm24815_chr12_7234513_R Primer3: not found at this Tm N.d. 0.33
mm4_intergenic_Ttll7|Gm23131_chr3_147226717_F Primer3: not found at this Tm N.d. 0.33
mm4_intergenic_Ttll7|Gm23131_chr3_147226717_R Primer3: not found at this Tm N.d. 0.33
mm4_intron_Gm9962_chr7_57402843_F Primer3: not found at this Tm N.d. 0.33
mm4_intron_Gm9962_chr7_57402843_R Primer3: not found at this Tm N.d. 0.33
mm4_intergenic_Cyp2b23|Cyp2b19_chr7_26711464_F Primer3: not found at this Tm N.d. 0.31
mm4_intergenic_Cyp2b23|Cyp2b19_chr7_26711464_R Primer3: not found at this Tm N.d. 0.31
mm3_intron_Fmn2_chr1_174663602_F Primer3: not found at this Tm N.d. 0.30
mm3_intron_Fmn2_chr1_174663602_R Primer3: not found at this Tm N.d. 0.30
mm3_intergenic_Apoo-ps|Zswim6_chr13_107503192_F Primer3: not found at this Tm N.d. 0.30
mm3_intergenic_Apoo-ps|Zswim6_chr13_107503192_R Primer3: not found at this Tm N.d. 0.30
mm4_intergenic_Gm4889|Dusp4_chr8_34686443_F Primer3: not found at this Tm N.d. 0.30
mm4_intergenic_Gm4889|Dusp4_chr8_34686443_R Primer3: not found at this Tm N.d. 0.30
mm4_intergenic_Chrne|Gm12315_chr11_70620573_F TCGTCGGCAGCGTCAGCCCTTTATGCGTTACACCT 59.7 0.30
mm4_intergenic_Chrne|Gm12315_chr11_70620573_R GTCTCGTGGGCTCGGAGTGACCCTCCGCTACAGAT 60.0 0.30
mm4_intergenic_Prrxl1|3425401B19Rik_chr14_32655187_F Primer3: not found at this Tm N.d. 0.30
mm4_intergenic_Prrxl1|3425401B19Rik_chr14_32655187_R Primer3: not found at this Tm N.d. 0.30
mm4_intergenic_Ccdc148|Gm13550_chr2_59045614_F Primer3: not found at this Tm N.d. 0.29
mm4_intergenic_Ccdc148|Gm13550_chr2_59045614_R Primer3: not found at this Tm N.d. 0.29
mm4_intron_Fam188a_chr2_12367266_F Primer3: not found at this Tm N.d. 0.28
mm4_intron_Fam188a_chr2_12367266_R Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_Gm14823|Gm14855_chrX_105664437_F Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_Gm14823|Gm14855_chrX_105664437_R Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_Gm4487|Gm24073_chr14_114444730_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm4487|Gm24073_chr14_114444730_R Primer3: not found at this Tm N.d. 0.27
mm4_intron_Dmd_chrX_84982329_F Primer3: not found at this Tm N.d. 0.27
mm4_intron_Dmd_chrX_84982329_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm24477|Trp53rk_chr2_166773444_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm24477|Trp53rk_chr2_166773444_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Slc1a3|Gm5210_chr15_8741319_F TCGTCGGCAGCGTCACGATGTGTGTGGAGACATGT 59.6 0.27
mm4_intergenic_Slc1a3|Gm5210_chr15_8741319_R GTCTCGTGGGCTCGGACAATCCATGACAGCTGCCA 59.9 0.27
mm4_intergenic_Cish/Gm17041|Hemk1_chr9_107307784_F TCGTCGGCAGCGTCGATTGGGAGGTGTGGGCTTT 60.2 0.25
mm4_intergenic_Cish/Gm17041|Hemk1_chr9_107307784_R GTCTCGTGGGCTCGGCTTCTCTGCCCTCCATGAGC 60.1 0.25
mm4_intergenic_Gm13402/Med27|Med27_chr2_29364247_F Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Gm13402/Med27|Med27_chr2_29364247_R Primer3: not found at this Tm N.d. 0.24
mm4_intron_Tceal3_chrX_136660268_F Primer3: not found at this Tm N.d. 0.24
mm4_intron_Tceal3_chrX_136660268_R Primer3: not found at this Tm N.d. 0.24
mm3_intergenic_D930016D06Rik|Gm8258_chr5_104697299_F Primer3: not found at this Tm N.d. 0.23
mm3_intergenic_D930016D06Rik|Gm8258_chr5_104697299_R Primer3: not found at this Tm N.d. 0.23
mm3_intergenic_D930016D06Rik|Gm8258_chr5_104640792_F Primer3: not found at this Tm N.d. 0.23
mm3_intergenic_D930016D06Rik|Gm8258_chr5_104640792_R Primer3: not found at this Tm N.d. 0.23
mm4_intergenic_Gm14513|Gm26314_chrX_11733130_F Primer3: not found at this Tm N.d. 0.23
mm4_intergenic_Gm14513|Gm26314_chrX_11733130_R Primer3: not found at this Tm N.d. 0.23
mm4_intergenic_Cntn5|Gm25365_chr9_11456616_F Primer3: not found at this Tm N.d. 0.23
mm4_intergenic_Cntn5|Gm25365_chr9_11456616_R Primer3: not found at this Tm N.d. 0.23
mm4_intergenic_Leo1|Tmod3_chr9_75481544_F TCGTCGGCAGCGTCAGGAGGCAGAAGCGGCAG 62.0 0.22
mm4_intergenic_Leo1|Tmod3_chr9_75481544_R GTCTCGTGGGCTCGGACGGCTTTTAGTGGCAACCT 60.1 0.22
mm4_intron_Kcnmb4_chr10_116446940_F TCGTCGGCAGCGTCTTCGATTTGAAACAATGGCAACA 58.3 0.22
mm4_intron_Kcnmb4_chr10_116446940_R GTCTCGTGGGCTCGGTGCCGTAACCTTATAACTCACAGT 59.7 0.22
mm4_intergenic_Sorcs3|AC116451.1_chr19_49418658_F Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Sorcs3|AC116451.1_chr19_49418658_R Primer3: not found at this Tm N.d. 0.21
mm4_intron_Zc3h6_chr2_129004885_F TCGTCGGCAGCGTCTGTGAGTTCTGTTCTTCTCCCT 58.6 0.21
mm4_intron_Zc3h6_chr2_129004885_R GTCTCGTGGGCTCGGGGAAGAACACAGACAAAGCAGT 59.0 0.21
mm4_intron_Cdk9_chr2_32708496_F Primer3: not found at this Tm N.d. 0.21
mm4_intron_Cdk9_chr2_32708496_R Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Gm12184/Trim7|Trim7_chr11_48832650_F Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Gm12184/Trim7|Trim7_chr11_48832650_R Primer3: not found at this Tm N.d. 0.20
mm4_intron_Lgr4_chr2_109944041_F TCGTCGGCAGCGTCTAGATGCACCTGGGCTTTGG 60.0 0.20
mm4_intron_Lgr4_chr2_109944041_R GTCTCGTGGGCTCGGACTAAAGTGAACCATTTCATGCC 57.6 0.20
mm4_intron_Zmat4_chr8_24087655_F TCGTCGGCAGCGTCTGGCCCCTACCACAGTATCA 59.9 0.20
mm4_intron_Zmat4_chr8_24087655_R GTCTCGTGGGCTCGGCAAATCTGTGATTCGGCTCTCT 58.4 0.20
mm4_intergenic_Nubpl|Arhgap5_chr12_52471758_F Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Nubpl|Arhgap5_chr12_52471758_R Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Gm5958|Klf12_chr14_99851237_F Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Gm5958|Klf12_chr14_99851237_R Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Olfr553|Olfr554_chr7_102639055_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Olfr553|Olfr554_chr7_102639055_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_n-R5s175|Gm2301_chr5_119313324_F TCGTCGGCAGCGTCATCCCCTCCCTTCCCAACTT 60.1 0.19
mm4_intergenic_n-R5s175|Gm2301_chr5_119313324_R GTCTCGTGGGCTCGGGGGGTCCAGTCTCTGCTCTA 60.0 0.19
mm4_intergenic_Adra2a|Gpam_chr19_54299779_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Adra2a|Gpam_chr19_54299779_R Primer3: not found at this Tm N.d. 0.19
mm4_intron_Slc37a1_chr17_31298558_F TCGTCGGCAGCGTCGTGCACTCCTTCCAGAATGGA 59.9 0.19
mm4_intron_Slc37a1_chr17_31298558_R GTCTCGTGGGCTCGGACAGTGCCAGACAGCCTTAA 59.2 0.19
mm4_intron_Cirh1a_chr8_106901894_F TCGTCGGCAGCGTCTGACTGTTTTGGGCTCTGCT 59.8 0.18
mm4_intron_Cirh1a_chr8_106901894_R GTCTCGTGGGCTCGGCGTGTGCTCGGACACTTATG 59.0 0.18
mm4_intergenic_Tas2r118|Slc13a1_chr6_24077015_F Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Tas2r118|Slc13a1_chr6_24077015_R Primer3: not found at this Tm N.d. 0.18
mm4_intron_Ptpra_chr2_130459217_F Primer3: not found at this Tm N.d. 0.18
mm4_intron_Ptpra_chr2_130459217_R Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Bsph2|RP24-317L6.1_chr7_13576408_F Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Bsph2|RP24-317L6.1_chr7_13576408_R Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Eif3b|Chst12_chr5_140492230_F Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Eif3b|Chst12_chr5_140492230_R Primer3: not found at this Tm N.d. 0.17
mm4_intron_Hk1_chr10_62319266_F Primer3: not found at this Tm N.d. 0.17
mm4_intron_Hk1_chr10_62319266_R Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Spef2|Gm26350_chr15_10009365_F Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Spef2|Gm26350_chr15_10009365_R Primer3: not found at this Tm N.d. 0.17
mm3_intergenic_5033426O07Rik|Gm26815_chr8_121222762_F Primer3: not found at this Tm N.d. 0.17
mm3_intergenic_5033426O07Rik|Gm26815_chr8_121222762_R Primer3: not found at this Tm N.d. 0.17
mm4_intron_Mcu_chr10_59514584_F Primer3: not found at this Tm N.d. 0.17
mm4_intron_Mcu_chr10_59514584_R Primer3: not found at this Tm N.d. 0.17
mm4_intron_Grin2b_chr6_135812714_F Primer3: not found at this Tm N.d. 0.17
mm4_intron_Grin2b_chr6_135812714_R Primer3: not found at this Tm N.d. 0.17
mm4_intron_Itga9_chr9_118624618_F Primer3: not found at this Tm N.d. 0.16
mm4_intron_Itga9_chr9_118624618_R Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Sv2c|Gm17190_chr13_96065547_F Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Sv2c|Gm17190_chr13_96065547_R Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Gm12723|Gm12726_chr4_105558449_F TCGTCGGCAGCGTCCATGTGCATGCCCCATTTCC 60.1 0.16
mm4_intergenic_Gm12723|Gm12726_chr4_105558449_R GTCTCGTGGGCTCGGTGAGTTACTGTGATGTCCTGGT 58.7 0.16
mm4_intron_Ush2a_chr1_188795780_F Primer3: not found at this Tm N.d. 0.16
mm4_intron_Ush2a_chr1_188795780_R Primer3: not found at this Tm N.d. 0.16
mm4_intron_Slc4a7_chr14_14735295_F TCGTCGGCAGCGTCCAACTCACCTTAGGCCTGCA 59.9 0.15
mm4_intron_Slc4a7_chr14_14735295_R GTCTCGTGGGCTCGGTCCTTCTTGCCATGTCTCACA 59.3 0.15
mm4_intron_Gfm2_chr13_97172101_F Primer3: not found at this Tm N.d. 0.15
mm4_intron_Gfm2_chr13_97172101_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Sntn|Rpl19-ps4/AC122290.1_chr14_13739204_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Sntn|Rpl19-ps4/AC122290.1_chr14_13739204_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_4933406K04Rik|1700013N06Rik_chr12_106723989_F TCGTCGGCAGCGTCGTTGGTGGGTACTTAGGGCC 60.0 0.15
mm4_intergenic_4933406K04Rik|1700013N06Rik_chr12_106723989_R GTCTCGTGGGCTCGGTGTTGGGATTCAGACAGCCT 58.9 0.15
mm4_intergenic_Gm14067|Gm14069_chr2_139082994_F TCGTCGGCAGCGTCTCAGAGGTGACAATGGAGTGT 58.6 0.15
mm4_intergenic_Gm14067|Gm14069_chr2_139082994_R GTCTCGTGGGCTCGGGCTGAAGCATTTGAATAGGCCT 59.3 0.15
mm4_intron_Nubpl_chr12_52135640_F Primer3: not found at this Tm N.d. 0.15
mm4_intron_Nubpl_chr12_52135640_R Primer3: not found at this Tm N.d. 0.15
mm4_intron_Gm15155_chrX_156186538_F Primer3: not found at this Tm N.d. 0.14
mm4_intron_Gm15155_chrX_156186538_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Gm15529|Gm5300_chr6_13041302_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Gm15529|Gm5300_chr6_13041302_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Elovl6|Egf_chr3_129640776_F TCGTCGGCAGCGTCCTGGGAGCCACTAGCCTCTA 60.1 0.14
mm4_intergenic_Elovl6|Egf_chr3_129640776_R GTCTCGTGGGCTCGGGAGATCCGGCAGATGTCAGG 59.9 0.14
mm4_exon_Irak3_chr10_120142309_F TCGTCGGCAGCGTCGAGTGACTGACTGCTGGCTT 59.9 0.14
mm4_exon_Irak3_chr10_120142309_R GTCTCGTGGGCTCGGAGCAGGACAAGTCTCTCTCT 57.3 0.14
mm3_intergenic_Lyzl6|Rprml_chr11_103644196_F Primer3: not found at this Tm N.d. 0.14
mm3_intergenic_Lyzl6|Rprml_chr11_103644196_R Primer3: not found at this Tm N.d. 0.14
mm4_intron_Erc2_chr14_27649363_F TCGTCGGCAGCGTCCTTGCAGACTCCCTGAGGTT 59.3 0.14
mm4_intron_Erc2_chr14_27649363_R GTCTCGTGGGCTCGGGGAGCTTGTCCTCCTCTGTC 59.4 0.14
mm4_intron_Ksr2_chr5_117611519_F Primer3: not found at this Tm N.d. 0.14
mm4_intron_Ksr2_chr5_117611519_R Primer3: not found at this Tm N.d. 0.14
mm4_intron_Rsrc1_chr3_67109647_F Primer3: not found at this Tm N.d. 0.13
mm4_intron_Rsrc1_chr3_67109647_R Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Nxnl2|Hist1h2al_chr13_51182371_F Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Nxnl2|Hist1h2al_chr13_51182371_R Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Clic6|2410124H12Rik_chr16_92488601_F Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Clic6|2410124H12Rik_chr16_92488601_R Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_4933416E14Rik|Tspan7_chrX_10427666_F TCGTCGGCAGCGTCGCCCATAAAACCATAATATACTCCC 57.3 0.13
mm4_intergenic_4933416E14Rik|Tspan7_chrX_10427666_R GTCTCGTGGGCTCGGCCCACCCCTATTAGACCCCA 60.0 0.13
mm4_intergenic_Gm5224|Gm5493_chr17_22737349_F TCGTCGGCAGCGTCAGACCTATAGGAAGCAGACGGA 59.8 0.13
mm4_intergenic_Gm5224|Gm5493_chr17_22737349_R GTCTCGTGGGCTCGGTTACTAGTGCTCTGTGCCGC 60.1 0.13
mm3_intergenic_Serpini1|Gm25846_chr3_75697346_F Primer3: not found at this Tm N.d. 0.12
mm3_intergenic_Serpini1|Gm25846_chr3_75697346_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm24295|Mef2c_chr13_82910569_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm24295|Mef2c_chr13_82910569_R Primer3: not found at this Tm N.d. 0.12
mm3_intergenic_Nek7|Lhx9_chr1_138796467_F Primer3: not found at this Tm N.d. 0.12
mm3_intergenic_Nek7|Lhx9_chr1_138796467_R Primer3: not found at this Tm N.d. 0.12
mm4_intron_Shank2_chr7_144217935_F TCGTCGGCAGCGTCCAGGCAGAAGAGGTCACCAG 60.0 0.11
mm4_intron_Shank2_chr7_144217935_R GTCTCGTGGGCTCGGCCAAGTTCAGTGGCATGCAC 60.0 0.11
mm3_intergenic_1700018B24Rik|Gm23511_chr3_48847018_F TCGTCGGCAGCGTCAAGTGAGTCACTGGAGCCTT 58.5 0.11
mm3_intergenic_1700018B24Rik|Gm23511_chr3_48847018_R GTCTCGTGGGCTCGGGGCTACTTTCTCAGCCTGCA 60.0 0.11
mm4_intergenic_Mup-ps19|Mup-ps20_chr4_61986658_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Mup-ps19|Mup-ps20_chr4_61986658_R Primer3: not found at this Tm N.d. 0.11
mm4_intron_Tnfrsf19_chr14_60978551_F TCGTCGGCAGCGTCTCAAAATAAGATCTCTCTGCTTTCC 57.1 0.11
mm4_intron_Tnfrsf19_chr14_60978551_R GTCTCGTGGGCTCGGAAATAGAGTCGTATCACTTCCCC 57.4 0.11
mm4_intergenic_Gm25705|Gm11922_chr4_31289739_F TCGTCGGCAGCGTCATCCCCTTGACCCCAAACTG 59.5 0.11
mm4_intergenic_Gm25705|Gm11922_chr4_31289739_R GTCTCGTGGGCTCGGGCGGGTATCAAATATATTGCTTTCC 58.6 0.11
mm4_intron_Rnf152_chr1_105304852_F Primer3: not found at this Tm N.d. 0.11
mm4_intron_Rnf152_chr1_105304852_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm12004|4930554G24Rik_chr11_14071048_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Gm12004|4930554G24Rik_chr11_14071048_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Gm23447|Gm23773_chr17_61072205_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Gm23447|Gm23773_chr17_61072205_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Gm11499|Gm11500_chr11_92491427_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Gm11499|Gm11500_chr11_92491427_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Zswim6|Gm22118_chr13_107803951_F TCGTCGGCAGCGTCGCCATTTGAAAGCCCAGACC 59.7 0.10
mm4_intergenic_Zswim6|Gm22118_chr13_107803951_R GTCTCGTGGGCTCGGTCGATGTGCTTGATTAGGGACA 59.5 0.10
mm4_intergenic_Gm23239|Ntrk3_chr7_78143548_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Gm23239|Ntrk3_chr7_78143548_R Primer3: not found at this Tm N.d. 0.10
mm4_intron_Dnah2_chr11_69490661_F Primer3: not found at this Tm N.d. 0.09
mm4_intron_Dnah2_chr11_69490661_R Primer3: not found at this Tm N.d. 0.09
mm4_intron_Tspan9_chr6_128138511_F Primer3: not found at this Tm N.d. 0.09
mm4_intron_Tspan9_chr6_128138511_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm24350|Gm13464_chr2_42912982_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm24350|Gm13464_chr2_42912982_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Xkr4|Gm1992_chr1_3454980_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Xkr4|Gm1992_chr1_3454980_R Primer3: not found at this Tm N.d. 0.09
mm3_intron_Klhdc1_chr12_69243255_F Primer3: not found at this Tm N.d. 0.09
mm3_intron_Klhdc1_chr12_69243255_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm26372|Adk_chr14_21312654_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm26372|Adk_chr14_21312654_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm9621|Gm24200_chr9_93404536_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm9621|Gm24200_chr9_93404536_R Primer3: not found at this Tm N.d. 0.09
mm4_intron_Igf1_chr10_87880571_F Primer3: not found at this Tm N.d. 0.09
mm4_intron_Igf1_chr10_87880571_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm22628|5033411D12Rik_chr13_16418551_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm22628|5033411D12Rik_chr13_16418551_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm12634|Gm12635_chr4_90377689_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm12634|Gm12635_chr4_90377689_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_St3gal1|Zfat_chr15_67200839_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_St3gal1|Zfat_chr15_67200839_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm13463|Lrp1b_chr2_42164111_F TCGTCGGCAGCGTCTCCAGCAAATCAGTGTGGAGA 59.3 0.08
mm4_intergenic_Gm13463|Lrp1b_chr2_42164111_R GTCTCGTGGGCTCGGGGGACTGTAATTGGTGGAGGA 59.0 0.08
mm3_intergenic_Gm9081|Avpr1a_chr10_122380290_F TCGTCGGCAGCGTCCCACTCTCTGCTGCCATGAT 59.8 0.08
mm3_intergenic_Gm9081|Avpr1a_chr10_122380290_R GTCTCGTGGGCTCGGGGTCACATGGTGTTGGATGG 58.8 0.08
mm4_intergenic_Efcab5|Ssh2_chr11_77215899_F TCGTCGGCAGCGTCACGTGTTCGCCTCAGGATTT 59.9 0.08
mm4_intergenic_Efcab5|Ssh2_chr11_77215899_R GTCTCGTGGGCTCGGCAACCGAGGGTCCTGATCTG 59.8 0.08
mm4_intergenic_Mettl21c|Gm8251_chr1_44055394_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Mettl21c|Gm8251_chr1_44055394_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Slc22a23|Pxdc1_chr13_34378611_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Slc22a23|Pxdc1_chr13_34378611_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Tmem141|Fcna_chr2_25624500_F TCGTCGGCAGCGTCGCACTTCTCTGTTCAGGGCT 59.9 0.08
mm4_intergenic_Tmem141|Fcna_chr2_25624500_R GTCTCGTGGGCTCGGCAGCACCAGGAAGGAGTCAG 60.0 0.08
mm3_intron_5730455P16Rik_chr11_80373743_F Primer3: not found at this Tm N.d. 0.07
mm3_intron_5730455P16Rik_chr11_80373743_R Primer3: not found at this Tm N.d. 0.07
mm3_intergenic_Gm24795|Gm8609_chr16_39865950_F Primer3: not found at this Tm N.d. 0.07
mm3_intergenic_Gm24795|Gm8609_chr16_39865950_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Cacna1e|Gm9530_chr1_154968720_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Cacna1e|Gm9530_chr1_154968720_R Primer3: not found at this Tm N.d. 0.07
mm4_intron_Zfat_chr15_68243306_F TCGTCGGCAGCGTCACATTCTCTGCCAGGCACAA 59.8 0.07
mm4_intron_Zfat_chr15_68243306_R GTCTCGTGGGCTCGGCAGGAGTCCTCAAAGCCAGG 60.0 0.07
mm4_exon_Ap3b1_chr13_94440239_F Primer3: not found at this Tm N.d. 0.07
mm4_exon_Ap3b1_chr13_94440239_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Nrg3|Gm10713_chr14_39901276_F TCGTCGGCAGCGTCTGGATTGTACTGTCAATTGGTCA 58.0 0.07
mm4_intergenic_Nrg3|Gm10713_chr14_39901276_R GTCTCGTGGGCTCGGAGAAGGATCAAAACTGTGGTTGA 58.2 0.07
mm3_intergenic_Gm24319|Psat1_chr19_15697952_F Primer3: not found at this Tm N.d. 0.07
mm3_intergenic_Gm24319|Psat1_chr19_15697952_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Dpyd|Gm23432_chr3_119614811_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Dpyd|Gm23432_chr3_119614811_R Primer3: not found at this Tm N.d. 0.07
mm3_intergenic_Otud7a|Klf13_chr7_63865601_F Primer3: not found at this Tm N.d. 0.07
mm3_intergenic_Otud7a|Klf13_chr7_63865601_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm4795|Prep_chr10_45049885_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm4795|Prep_chr10_45049885_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm10356|Gm5274_chr3_47060258_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm10356|Gm5274_chr3_47060258_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Atp6v1g3|Nek7_chr1_138467897_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Atp6v1g3|Nek7_chr1_138467897_R Primer3: not found at this Tm N.d. 0.06
mm3_intergenic_Bri3|Nptx2_chr5_144487843_F Primer3: not found at this Tm N.d. 0.06
mm3_intergenic_Bri3|Nptx2_chr5_144487843_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Mtif3|Lnx2_chr5_146968896_F TCGTCGGCAGCGTCCGCTGTCCATTTGGGGTCAT 60.6 0.06
mm4_intergenic_Mtif3|Lnx2_chr5_146968896_R GTCTCGTGGGCTCGGCATAGGGCTGCCAACAAAGC 59.8 0.06
mm4_intron_Sulf1_chr1_12775560_F TCGTCGGCAGCGTCGGCACATGCAGCAGTTTTCT 59.6 0.06
mm4_intron_Sulf1_chr1_12775560_R GTCTCGTGGGCTCGGCCTGTGAAGCTTCTGTGTCT 57.4 0.06
mm4_intron_Pcx_chr19_4591158_F TCGTCGGCAGCGTCACCTTTGCCCTGCCTCATTT 60.1 0.06
mm4_intron_Pcx_chr19_4591158_R GTCTCGTGGGCTCGGTCACCTCATCCTGCTCCAGT 60.2 0.06
mm4_intergenic_Trpc7|Gm22777_chr13_56925827_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Trpc7|Gm22777_chr13_56925827_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm25722|Gm10051_chr5_133067033_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm25722|Gm10051_chr5_133067033_R Primer3: not found at this Tm N.d. 0.06
mm3_intergenic_Gm24404|Nrg3_chr14_37836176_F Primer3: not found at this Tm N.d. 0.06
mm3_intergenic_Gm24404|Nrg3_chr14_37836176_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Orai2_chr5_136169471_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Orai2_chr5_136169471_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Atp11a_chr8_12773560_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Atp11a_chr8_12773560_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Hs6st2_chrX_51468860_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Hs6st2_chrX_51468860_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm14643|Gm26194_chrX_39928246_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm14643|Gm26194_chrX_39928246_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Efcab4b|Prmt8_chr6_127670931_F TCGTCGGCAGCGTCCTTTTCTCACAAGCTGGCCC 59.4 0.05
mm4_intergenic_Efcab4b|Prmt8_chr6_127670931_R GTCTCGTGGGCTCGGAGTGGCTCTCGTGCTTCTTC 60.0 0.05
mm4_intergenic_Gm23503|Fign_chr2_63869312_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm23503|Fign_chr2_63869312_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_AI429214|Gm25126_chr8_37231312_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_AI429214|Gm25126_chr8_37231312_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_4631405K08Rik|Plxna2_chr1_194565258_F TCGTCGGCAGCGTCCAAGAGGAAGCACTGCCAGA 59.9 0.05
mm4_intergenic_4631405K08Rik|Plxna2_chr1_194565258_R GTCTCGTGGGCTCGGACCCTGGGAAGATGTCCTTA 57.3 0.05
mm4_intergenic_Sergef|Gm15700_chr7_46539853_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Sergef|Gm15700_chr7_46539853_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Fam110b_chr4_5677876_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Fam110b_chr4_5677876_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Ndst4|Ugt8a_chr3_125827358_F TCGTCGGCAGCGTCTCAGTTCCAGGCAGGGTACT 60.1 0.05
mm4_intergenic_Ndst4|Ugt8a_chr3_125827358_R GTCTCGTGGGCTCGGGACTGGCAGGGGTTGTCTTG 60.8 0.05
mm4_intergenic_Nubpl|Arhgap5_chr12_52386405_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Nubpl|Arhgap5_chr12_52386405_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm16958|Ank2_chr3_127195304_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm16958|Ank2_chr3_127195304_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Gm5483_chr16_36185827_F TCGTCGGCAGCGTCGGGAGGCAGAGAAACAGACA 59.3 0.05
mm4_intron_Gm5483_chr16_36185827_R GTCTCGTGGGCTCGGGGGATTTCCATAATATGTGCCCC 59.2 0.05
mm4_intergenic_Gm23339|Gm25570_chr6_105248035_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm23339|Gm25570_chr6_105248035_R Primer3: not found at this Tm N.d. 0.05
mm3_intron_1700007B14Rik_chr8_75775140_F Primer3: not found at this Tm N.d. 0.05
mm3_intron_1700007B14Rik_chr8_75775140_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm15219|Cdkn3_chr14_46623957_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm15219|Cdkn3_chr14_46623957_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm26137|Gm23733_chr3_120104070_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm26137|Gm23733_chr3_120104070_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Rab31_chr17_65684069_F TCGTCGGCAGCGTCACAGCAGGCTTCTCCTTTCT 58.9 0.04
mm4_intron_Rab31_chr17_65684069_R GTCTCGTGGGCTCGGAGCATATTTGAGGCCCAGCA 59.7 0.04
mm4_intron_Golga7b_chr19_42249897_F TCGTCGGCAGCGTCGATGAAGTGCCGTGCTTGTG 60.1 0.04
mm4_intron_Golga7b_chr19_42249897_R GTCTCGTGGGCTCGGTCGTCAGTTCCTTCAGTCAGC 60.0 0.04
mm4_intron_Rad18_chr6_112677905_F TCGTCGGCAGCGTCAGCAGGTCACAAGATACCGA 58.4 0.04
mm4_intron_Rad18_chr6_112677905_R GTCTCGTGGGCTCGGACCCACAGTTTGTTGATGGGA 59.7 0.04
mm4_intron_Sdk2_chr11_113844983_F TCGTCGGCAGCGTCAAAGTACCCACCTTCCTGCC 59.5 0.04
mm4_intron_Sdk2_chr11_113844983_R GTCTCGTGGGCTCGGACCCTGCACCATAAATTCATCA 58.0 0.04
mm4_intergenic_Gm23434|Cntnap5b_chr1_99355106_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm23434|Cntnap5b_chr1_99355106_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Alcam|Gm25723_chr16_52596586_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Alcam|Gm25723_chr16_52596586_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm25255|Csmd1_chr8_15848596_F TCGTCGGCAGCGTCACACACAGCCTATTCACGAGA 59.1 0.03
mm4_intergenic_Gm25255|Csmd1_chr8_15848596_R GTCTCGTGGGCTCGGGCCTCAGTTTCACCACACGA 60.5 0.03
mm3_intron_Pde1c_chr6_56116771_F Primer3: not found at this Tm N.d. 0.03
mm3_intron_Pde1c_chr6_56116771_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm26651|Diras2_chr13_51978542_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm26651|Diras2_chr13_51978542_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Tmtc1|Gm6313_chr6_148552469_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Tmtc1|Gm6313_chr6_148552469_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Arhgef3_chr14_27286013_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Arhgef3_chr14_27286013_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Tpt1-ps2|Gm12478_chr4_54622543_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Tpt1-ps2|Gm12478_chr4_54622543_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Egfem1_chr3_29355167_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Egfem1_chr3_29355167_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Kcnh8|Efhb_chr17_53335919_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Kcnh8|Efhb_chr17_53335919_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Ppp1r12b_chr1_134887091_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Ppp1r12b_chr1_134887091_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm24396|Rit2_chr18_30903199_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm24396|Rit2_chr18_30903199_R Primer3: not found at this Tm N.d. 0.03
mm3_intron_Wdr96_chr19_47817445_F Primer3: not found at this Tm N.d. 0.03
mm3_intron_Wdr96_chr19_47817445_R Primer3: not found at this Tm N.d. 0.03
mm4_exon_Lpgat1_chr1_191760054_F TCGTCGGCAGCGTCCCAACAGCTGCTGGTTCTCA 60.5 0.03
mm4_exon_Lpgat1_chr1_191760054_R GTCTCGTGGGCTCGGTGGCTTGTTTCTCGCCTCTT 59.8 0.03
mm4_intergenic_Cdh7|Cdh19_chr1_110478211_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Cdh7|Cdh19_chr1_110478211_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_mmu-mir-6373|Gm26022_chr6_102807076_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_mmu-mir-6373|Gm26022_chr6_102807076_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Atp6v0a1_chr11_101040348_F TCGTCGGCAGCGTCCCACCACCACAAACAAACAAAC 59.2 0.03
mm4_intron_Atp6v0a1_chr11_101040348_R GTCTCGTGGGCTCGGTGTAAGTCAGTTTTGGGGTTTGT 58.6 0.03
mm4_intergenic_Fbxo15|Gm25453_chr18_85962005_F TCGTCGGCAGCGTCTCCACCCAACATGTGAGGTT 59.1 0.03
mm4_intergenic_Fbxo15|Gm25453_chr18_85962005_R GTCTCGTGGGCTCGGTGACTCCTGTACTTAAACACGG 57.7 0.03
mm4_intron_Cdc6_chr11_98916849_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Cdc6_chr11_98916849_R Primer3: not found at this Tm N.d. 0.02
mm3_intergenic_Gm15212|Gm15214_chrX_163293655_F Primer3: not found at this Tm N.d. 0.02
mm3_intergenic_Gm15212|Gm15214_chrX_163293655_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Epha6|Gm24755_chr16_60225287_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Epha6|Gm24755_chr16_60225287_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Hace1_chr10_45655125_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Hace1_chr10_45655125_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Ppargc1b|Gm25834_chr18_61445263_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Ppargc1b|Gm25834_chr18_61445263_R Primer3: not found at this Tm N.d. 0.02
mm3_intron_Nckap5_chr1_126409085_F Primer3: not found at this Tm N.d. 0.02
mm3_intron_Nckap5_chr1_126409085_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Gm26520_chr12_3356790_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Gm26520_chr12_3356790_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm25384|Cxcr4_chr1_128515645_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm25384|Cxcr4_chr1_128515645_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Igkv10-95|Igkv10-94_chr6_68687182_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Igkv10-95|Igkv10-94_chr6_68687182_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Spesp1|Anp32a_chr9_62317519_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Spesp1|Anp32a_chr9_62317519_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Mapre1_chr2_153766536_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Mapre1_chr2_153766536_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Fam180a|Mtpn_chr6_35480753_F TCGTCGGCAGCGTCACAAAACACCTAAGGCGAGG 58.1 0.02
mm4_intergenic_Fam180a|Mtpn_chr6_35480753_R GTCTCGTGGGCTCGGAGCCATGGAGCTCACAAAGT 59.5 0.02
mm4_intron_E230008N13Rik_chr4_45933898_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_E230008N13Rik_chr4_45933898_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Pkd2l2_chr18_34436959_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Pkd2l2_chr18_34436959_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Fam110b|Gm11796_chr4_5827951_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Fam110b|Gm11796_chr4_5827951_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Mut|Gm6771_chr17_41225277_F TCGTCGGCAGCGTCACAGCAGATTAACAGCCCCA 59.3 0.01
mm4_intergenic_Mut|Gm6771_chr17_41225277_R GTCTCGTGGGCTCGGGTTGCCCTATGCGACCTTAGT 60.1 0.01
mm4_intron_Rcor1_chr12_111062402_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Rcor1_chr12_111062402_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Ptgis/1110018N20Rik|Gm14291_chr2_167194530_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Ptgis/1110018N20Rik|Gm14291_chr2_167194530_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Zfp318_chr17_46389028_F TCGTCGGCAGCGTCAGGTTGTAGCGCCATTAGCA 59.7 0.01
mm4_intron_Zfp318_chr17_46389028_R GTCTCGTGGGCTCGGTGGTCTATTCAATTAGCCGAAGACT 59.8 0.01
mm4_exon_Mpp6_chr6_50110998_F TCGTCGGCAGCGTCGTGGATTGGTTGGGGTGTCT 59.8 0.01
mm4_exon_Mpp6_chr6_50110998_R GTCTCGTGGGCTCGGTGAATGTTTTCTCGAGCTGCG 59.5 0.01
mm4_intron_Slc26a7_chr4_14601018_F TCGTCGGCAGCGTCTGGCACAGGGTCTTAAGTATGA 58.8 0.01
mm4_intron_Slc26a7_chr4_14601018_R GTCTCGTGGGCTCGGAAGATGCAGTCTCCTGGCTG 59.7 0.01
mm3_intergenic_Vmn2r17|Gm8488_chr5_109522832_F Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Vmn2r17|Gm8488_chr5_109522832_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Calcrl_chr2_84373421_F TCGTCGGCAGCGTCTTCACGCCTTCTTCCGACTC 60.0 0.01
mm4_intron_Calcrl_chr2_84373421_R GTCTCGTGGGCTCGGGCACCTCTGCTCTTGCTGTT 60.8 0.01
mm3_intergenic_Gm23892|Gm25382_chr10_16758786_F Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Gm23892|Gm25382_chr10_16758786_R Primer3: not found at this Tm N.d. 0.01
mm3_intron_Pax7_chr4_139827792_F Primer3: not found at this Tm N.d. 0.01
mm3_intron_Pax7_chr4_139827792_R Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Dync1i1|Slc25a13_chr6_6031241_F Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Dync1i1|Slc25a13_chr6_6031241_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Ceacam10|Gm5893_chr7_24810001_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Ceacam10|Gm5893_chr7_24810001_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm22254|Gm8993_chr6_74803402_F TCGTCGGCAGCGTCGCCCTACAGGAAACATTGCC 59.1 0.01
mm4_intergenic_Gm22254|Gm8993_chr6_74803402_R GTCTCGTGGGCTCGGTGCTGGCACTTGTATTGATATACA 58.3 0.01
mm4_intron_Dtnb_chr12_3579550_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Dtnb_chr12_3579550_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Syn3_chr10_86216209_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Syn3_chr10_86216209_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Pip5k1b_chr19_24344215_F TCGTCGGCAGCGTCCTTACCCACCCACCATCACC 60.0 0.00
mm4_intron_Pip5k1b_chr19_24344215_R GTCTCGTGGGCTCGGTGGTATTTGGGGTTGGGGTT 59.1 0.00
mm4_intergenic_Gm5641|Gm26678_chr3_54559062_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm5641|Gm26678_chr3_54559062_R Primer3: not found at this Tm N.d. 0.00
mm3_intergenic_Ube2nl|Gm25533_chr7_61650468_F Primer3: not found at this Tm N.d. 0.00
mm3_intergenic_Ube2nl|Gm25533_chr7_61650468_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Zfp207_chr11_80402754_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Zfp207_chr11_80402754_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Unc5d|4933433F19Rik_chr8_29450200_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Unc5d|4933433F19Rik_chr8_29450200_R Primer3: not found at this Tm N.d. 0.00
mm2_intergenic_Lilra6|Gm7363_chr7_3983025_F Primer3: not found at this Tm N.d. 0.00
mm2_intergenic_Lilra6|Gm7363_chr7_3983025_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Rbms3|Gm26614_chr9_117824180_F TCGTCGGCAGCGTCCGGCTTGAACAGAATTCGGT 58.8 0.00
mm4_intergenic_Rbms3|Gm26614_chr9_117824180_R GTCTCGTGGGCTCGGAGGAGGGAGTAGCCATACAGG 60.1 0.00
mm4_intron_Tenm4_chr7_96844135_F TCGTCGGCAGCGTCCCTGGACATCCCCTGTTGAG 59.7 0.00
mm4_intron_Tenm4_chr7_96844135_R GTCTCGTGGGCTCGGGACAGGAGACAGGCGTTGAA 59.9 0.00
mm4_intron_Scaper_chr9_55594362_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Scaper_chr9_55594362_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Robo1_chr16_72867275_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Robo1_chr16_72867275_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Oxsr1_chr9_119297852_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Oxsr1_chr9_119297852_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Mapre2_chr18_23872163_F TCGTCGGCAGCGTCAGGAGAGGGAGGAACAGTCC 59.9 0.00
mm4_intron_Mapre2_chr18_23872163_R GTCTCGTGGGCTCGGGTGTTAAGTATATGGGCACACGC 59.9 0.00
mm4_intron_Grid1_chr14_35129455_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Grid1_chr14_35129455_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Gpd2_chr2_57345502_F TCGTCGGCAGCGTCGCAACTTGCTGGGCCTTTC 60.0 0.00
mm4_intron_Gpd2_chr2_57345502_R GTCTCGTGGGCTCGGGTGGGGAGGAAAAGGCTGAG 60.3 0.00
mm4_intron_Gm5432_chr12_15861906_F TCGTCGGCAGCGTCGGAGTCAGGCAACAGCAGAT 60.0 0.00
mm4_intron_Gm5432_chr12_15861906_R GTCTCGTGGGCTCGGGGCCAAGGATAGGGAAAAGGG 60.4 0.00
mm4_intron_Fyn_chr10_39470707_F TCGTCGGCAGCGTCGTGCATACACAGCATACGTGT 59.0 0.00
mm4_intron_Fyn_chr10_39470707_R GTCTCGTGGGCTCGGACCAAAGCAGGACTGAAGCA 59.8 0.00
mm4_intron_B3galt1_chr2_67889828_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_B3galt1_chr2_67889828_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Slc35f1|Gm25664_chr10_52879267_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Slc35f1|Gm25664_chr10_52879267_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_RP23-18M12.1|Tinag_chr9_76950741_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_RP23-18M12.1|Tinag_chr9_76950741_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Pitx1|H2afy_chr13_56015700_F TCGTCGGCAGCGTCAGGAGAATTGAGAGAGACTGTCA 58.3 0.00
mm4_intergenic_Pitx1|H2afy_chr13_56015700_R GTCTCGTGGGCTCGGGTCTGGTTTACACTGAGCTGGA 59.9 0.00
mm4_intergenic_Mtpn|Gm23273_chr6_35785808_F TCGTCGGCAGCGTCCCAGATGACAAGGGAGCCTC 59.8 0.00
mm4_intergenic_Mtpn|Gm23273_chr6_35785808_R GTCTCGTGGGCTCGGGGGTGAAAATACACACAATTTCAGA 58.2 0.00
mm4_intergenic_Gm8183|Tmem135_chr7_88881942_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm8183|Tmem135_chr7_88881942_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm26878|Fendrr_chr8_121043439_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm26878|Fendrr_chr8_121043439_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25976|Acot10_chr15_20378920_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25976|Acot10_chr15_20378920_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25656|Cntn6_chr6_103962239_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25656|Cntn6_chr6_103962239_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23965|Gm23537_chr1_103823917_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23965|Gm23537_chr1_103823917_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23056|Thsd7b_chr1_129253466_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23056|Thsd7b_chr1_129253466_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm14740|Gm14739_chrX_82566908_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm14740|Gm14739_chrX_82566908_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm10855|Gm10115_chr2_6945362_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm10855|Gm10115_chr2_6945362_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm10803|Alx4_chr2_93630114_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm10803|Alx4_chr2_93630114_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Fmn1|Grem1_chr2_113743362_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Fmn1|Grem1_chr2_113743362_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Dscaml1|Cep164_chr9_45753697_F TCGTCGGCAGCGTCCCAAGAAAACCGGGAGTTGC 59.6 0.00
mm4_intergenic_Dscaml1|Cep164_chr9_45753697_R GTCTCGTGGGCTCGGGCCCTTGCCAGATGGGAAAA 60.9 0.00
mm4_intergenic_Cyp2c37|Cyp2c54_chr19_40013158_F TCGTCGGCAGCGTCACAGATTTAAAATCAGACTTGCCA 57.1 0.00
mm4_intergenic_Cyp2c37|Cyp2c54_chr19_40013158_R GTCTCGTGGGCTCGGACCTTTGTTGCTGGCTTTGA 58.5 0.00
mm4_intergenic_Ctbp2|Tex36_chr7_133145960_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Ctbp2|Tex36_chr7_133145960_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Cep120|Csnk1g3_chr18_53847122_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Cep120|Csnk1g3_chr18_53847122_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Ccng1|Gm24446_chr11_40940368_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Ccng1|Gm24446_chr11_40940368_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_4931417E11Rik|Gm26179_chr6_73631806_F TCGTCGGCAGCGTCGCTTAAAGGAGGAGAGAGCTGA 59.2 0.00
mm4_intergenic_4931417E11Rik|Gm26179_chr6_73631806_R GTCTCGTGGGCTCGGAACTGTCCCGAAGCTCTGAC 59.6 0.00
mm4_intergenic_1700112E06Rik|Gm10248_chr14_22915664_F TCGTCGGCAGCGTCAGAGCACTGGCAAACCTTGA 59.8 0.00
mm4_intergenic_1700112E06Rik|Gm10248_chr14_22915664_R GTCTCGTGGGCTCGGCCTAACGCTTCCTTGGCTCA 60.0 0.00
mm3_intron_Sgms1_chr19_32212661_F Primer3: not found at this Tm N.d. 0.00
mm3_intron_Sgms1_chr19_32212661_R Primer3: not found at this Tm N.d. 0.00
mm3_intron_Mllt1_chr17_56931521_F Primer3: not found at this Tm N.d. 0.00
mm3_intron_Mllt1_chr17_56931521_R Primer3: not found at this Tm N.d. 0.00
mm3_intron_Mcm9_chr10_53625053_F Primer3: not found at this Tm N.d. 0.00
mm3_intron_Mcm9_chr10_53625053_R Primer3: not found at this Tm N.d. 0.00
mm3_intron_Eml1_chr12_108418714_F Primer3: not found at this Tm N.d. 0.00
mm3_intron_Eml1_chr12_108418714_R Primer3: not found at this Tm N.d. 0.00
mm3_intergenic_Nolc1|Elovl3_chr19_46124280_F Primer3: not found at this Tm N.d. 0.00
mm3_intergenic_Nolc1|Elovl3_chr19_46124280_R Primer3: not found at this Tm N.d. 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

mm3_intergenic_D730003I15Rik|Nenf_chr1_191241422 TGCCTGCTCTGACTTGGTAGCTAGCTCTTCTAAGTTCTCTACATTGCTTTGGAAGTTTTAGCCTATAAACTCAGGAGGAA
GATCTAAGAAAAAGCCCTCCATCCAGAAACAAAGCCAACTCAGTCGTAGGTACTCCAGCA
mm3_intergenic_C1galt1c1|Ap3s1-ps1_chrX_38673917 GAAGATGCTCAGAGCCTCCCCAGAGTTATGTTTCTGGAAGGAGGCTCTCAGGATCTGACTGAATAGTGGAATGATGACCC
CGTGT
mm3_intergenic_Nr5a2|Gm23763_chr1_136969416 GGTTCCCTTGTAGGCTGTTCACTTGTTGGTTATGTGCCTGGATGGGGGTGGAGCCCATAGCTTTGTGCATAGCTGGTGAG
TGCCTTCCAGTGGGTTT
mm4_intergenic_Chrne|Gm12315_chr11_70620573 AGCCCTTTATGCGTTACACCTTATGCATCACCCCAATCCAGGAAAATAATCATGTCTGTCCTGAATTTGTGAAAAGATCT
GTAGCGGAGGGTCACT
mm4_intergenic_Slc1a3|Gm5210_chr15_8741319 ACGATGTGTGTGGAGACATGTAGTGACCACTAGAGCATGAGTATGGTTCTGGATGAGGGAAGATAGGAGCTGACCTCTTA
AAGAACTGACCTCCCGGCAGGCTTTCTGAGGAAGAACCTGGCAGCTGTCATGGATTGT
mm4_intergenic_Cish/Gm17041|Hemk1_chr9_107307784 GATTGGGAGGTGTGGGCTTTGGAGACAACAGCGCCCCCCATCCAACAACTTGACCATCCCTAGTCTCTCTCCTGGGTACC
AGCAGGTTACCTGTGGGTGCTCATGGAGGGCAGAGAAG
mm4_intergenic_Leo1|Tmod3_chr9_75481544 AGGAGGCAGAAGCGGCAGCAGCGTCCAGGTCGGAGTTCCACCTTCCAGAAGCCTAACCTTGTTGTTNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNGCAAGGCCTCCATCGAGGTTGCCACTAAAAGCCGT
mm4_intron_Kcnmb4_chr10_116446940 TTCGATTTGAAACAATGGCAACAAACAAACAAACAGAAAACCCCAATCTAGTAACAAAACCATCTCCAGTCACTAAGAAG
TAAGGACATTTTATTAACTGTGAGTTATAAGGTTACGGCA
mm4_intron_Zc3h6_chr2_129004885 TGTGAGTTCTGTTCTTCTCCCTAAAAAGTGCATAAACATCACAATGTACAAAACCTCTCATTCCCACCATCTAGAAACAT
ATGCTT
GTAGCTTCTCTGGCCTATTTTTCTCTCTTTTTACTGCTTTGTCTGTGTTCTTCC
mm4_intron_Lgr4_chr2_109944041 TAGATGCACCTGGGCTTTGGGCATATGGGGGTCTGGGATGACTTCATTGTAATGTTTTTGGATTGTGGGGCATGAAATGG
TTCACTTTAGT
mm4_intron_Zmat4_chr8_24087655 TGGCCCCTACCACAGTATCATCTTTTCACTGGTAACGGGATATAGTCCTGAGTAATGATTCTTCCAGTCTACTAGGAATG
TAAGATGAGCAAATGGAAATGTCTTTGGATGGTGGTAAAGAGAGCCGAATCACAGATTTG
mm4_intergenic_n-R5s175|Gm2301_chr5_119313324 ATCCCCTCCCTTCCCAACTTCCCTCCATCCTGAGATCTAACCATTAAGGCTCATGAAATAACACTGGATGAGGGCTCTCA
CACATCTGCAGTGTTTTAGAGCAGAGACTGGACCCC
mm4_intron_Slc37a1_chr17_31298558 GTGCACTCCTTCCAGAATGGATGCCCAACCCCCATCCAGCTACATCATCATCTCAGTGAAATGGCTAGCATTATTTATAG
AAGCCAGGAGGGTTTTTTTTTTTCCCTGATAACTCTTTAAGGCTGTCTGGCACTGT
mm4_intron_Cirh1a_chr8_106901894 TGACTGTTTTGGGCTCTGCTATACATGACATGTTCATGATTCTGGATTGTGGTTTTTAATCTCTGTACGTTATTGACTTC
AACACCTGAGTCTTCCCTTTGTACCTTGTGTCATAAGTGTCCGAGCACACG
mm4_intergenic_Gm12723|Gm12726_chr4_105558449 CATGTGCATGCCCCATTTCCAATGAGCCATTGTTGCTCCCCCCATCCAGAAACAGAATATTTTTGTATACTATGAAACCA
GGACATCACAGTAACTCA
mm4_intron_Slc4a7_chr14_14735295 CAACTCACCTTAGGCCTGCATTTTGAAACTGTTCTCTAGTTTGACAGAATTTGAAAAATAATAGTTCTGTCTCTGGATAG
TGG
GTAGCCCAGACTCTGAATTGTGAGACATGGCAAGAAGGA
mm4_intergenic_4933406K04Rik|1700013N06Rik_chr12_106723989 GTTGGTGGGTACTTAGGGCCCATGACACAGCTGAACCTTTGCTGTAAGACCCCCACACATGCAGTGGTCATGTTTCTAGC
TGGTGG
CTAGGCTGTCTGAATCCCAACA
mm4_intergenic_Gm14067|Gm14069_chr2_139082994 TCAGAGGTGACAATGGAGTGTTACAAATATTTATTTTCTGACTGATCGTGAAGATTCTGGATGGTGGCTTGTCGGTTGAG
TTTCTGAAGTTCTGTCTCAACAAGACAGGCCTATTCAAATGCTTCAGC
mm4_intergenic_Elovl6|Egf_chr3_129640776 CTGGGAGCCACTAGCCTCTAAGGGCTTTGTTTTGTTTCTGGATTGCGGTCCCACACAGATTCCCCTGACATCTGCCGGAT
CTC
mm4_exon_Irak3_chr10_120142309 GAGTGACTGACTGCTGGCTTGGGGAAAAGGTGGTTCTGGTTCTGGAAGGTGGGTGGTTCTCTGTTCACCTATACCAAGAT
CTCACTCGGATGATGAGAGAGAGTGAGAGACTTAAGAGAGAGACTTGTCCTGCT
mm4_intron_Erc2_chr14_27649363 CTTGCAGACTCCCTGAGGTTAAATCCACACAGGTGTACACTTTGTGTTAGTTGTGAGAGCTTTCAAACCTAGCTAGAGAA
ACGAGATGTTTCTGTTTCTAGATGAAGGAGGAGAAGACAGAGGAGGACAAGCTCC
mm4_intergenic_4933416E14Rik|Tspan7_chrX_10427666 GCCCATAAAACCATAATATACTCCCTCCTATGTCCACTTTCCAGAAATAGAACCATATGAAACTCATTTTCCATTTGATC
TCAAAGAATAGGTCACACAAGTGGGGACTTGGAGAACAATGGGGTCTAATAGGGGTGGG
mm4_intergenic_Gm5224|Gm5493_chr17_22737349 AGACCTATAGGAAGCAGACGGAAGTCGAAGCAGATAAAGTTCAATGTCCACCATTGAGAAAGATGACCATGAGCGGCACA
GAGCACTAGTAA
mm4_intron_Shank2_chr7_144217935 CAGGCAGAAGAGGTCACCAGCACCCCCCGCACTCCCCACTGGTGTGATGGTAATGGTTATGGATAGGGGTGGTTGAGCTT
TGTTTGTGCATGCCACTGAACTTGG
mm3_intergenic_1700018B24Rik|Gm23511_chr3_48847018 AAGTGAGTCACTGGAGCCTTAATAATACACTGGCTTTTTGAGCATTAATGTTCTTAAAATAGCCAGGCACTTAAAGCCCT
TCCATCCAGAAACATTAGCAC
CTAAGCATCTTGCTGCAGGCTGAGAAAGTAGCC
mm4_intron_Tnfrsf19_chr14_60978551 TCAAAATAAGATCTCTCTGCTTTCCTTTTTCTGTCTATAGCCCTGTCTATATTTTAGAAGTTGATGTTTCTGGAAGGAGG
AAAGGGAAGAGGGGGAAGTGATACGACTCTATTT
mm4_intergenic_Gm25705|Gm11922_chr4_31289739 ATCCCCTTGACCCCAAACTGCAATTCCTTCATGTTTATTTTAATTTTTAATTACTTCTCACCAACTTCCAGAGACATAAA
CAT
CTTTTTTAAGGGAAAGCAATATATTTGATACCCGC
mm4_intergenic_Zswim6|Gm22118_chr13_107803951 GCCATTTGAAAGCCCAGACCTGAGCCCAGGTTATGGGTCTTTACATGCTGGGCGGCCTTCAACACCCCCAACCAGCATCC
AGAAAGATAAGCTT
GTTGCTGAAAACTGACCATCTTGTCCCTAATCAAGCACATCGA
mm4_intergenic_Gm13463|Lrp1b_chr2_42164111 TCCAGCAAATCAGTGTGGAGAGAACCCTTTCACCAACATCCACAAACATGACCACAATATTATGAGAGTTAGGCTATCCT
CCACCAATTACAGTCCC
mm3_intergenic_Gm9081|Avpr1a_chr10_122380290 CCACTCTCTGCTGCCATGATGTGGGTTTTAGTAACCTCAATGACCTAATTCTTTCAAAGTTATGTTTCTGGTTGGTGGTG
CTAATTATTTCACGACCTTTGTATTTTCCATCCATCCAACACCATGTGACC
mm4_intergenic_Efcab5|Ssh2_chr11_77215899 ACGTGTTCGCCTCAGGATTTTCGCCTCAGTTTTTCTCTAAGGTTGCGCGCCCCACTCATAAAATGGTTGTCTTTCTGGAG
TGAGG
ATTAGCGGCTCTCCCACCGACCCCCACCCCCAGATCAGGACCCTCGGTTG
mm4_intergenic_Tmem141|Fcna_chr2_25624500 GCACTTCTCTGTTCAGGGCTACAGCAGGGACCGAGTTGGTGTCGAGTAGGCCTTTCCACTCATTAATGGGTTTGTTTCTG
GAAGCGGG
TCTTGGAAGGCTGAGCTGACTCCTTCCTGGTGCTG
mm4_intron_Zfat_chr15_68243306 ACATTCTCTGCCAGGCACAATTAGAAATGAGAAGGATCCAGGCCTCCCTCCAGAAACACAAACGTCTGTGTCGTCTGGGG
CCTGGCTTTGAGGACTCCTG
mm4_intergenic_Nrg3|Gm10713_chr14_39901276 TGGATTGTACTGTCAATTGGTCACCTCCCTTCAGAACCAGAACCATGGCTGTTCTAAGCCTTTTTCAACCACAGTTTTGA
TCCTTCT
mm4_intergenic_Mtif3|Lnx2_chr5_146968896 CGCTGTCCATTTGGGGTCATTGTTTAACACTCTCCCCCCTCCAGAAAAAAAACCCTGCTAAGTTCCCCAGGCAAAGAGTT
TTAAAGGCTGCTTTGTTGGCAGCCCTATG
mm4_intron_Sulf1_chr1_12775560 GGCACATGCAGCAGTTTTCTTCCATGATTTTGGAAATCTTTGATTTACAGTAGCTTGGCCTTCCATCCAGAAACGTATCA
AA
TGTGATCCTGGATACTTAATTGTGACTAAATACAATCAAGACACAGAAGCTTCACAGG
mm4_intron_Pcx_chr19_4591158 ACCTTTGCCCTGCCTCATTTGGCATTTTGTTGTAGGCACCATTTCAGCTCTATGGTTTTCTGTCTGGAAGGAGGAATGCT
GAGTTCATAGATCCAGTGTTGGAGAAAACTGGAGCAGGATGAGGTGA
mm4_intergenic_Efcab4b|Prmt8_chr6_127670931 CTTTTCTCACAAGCTGGCCCCACACCTCAGGCTCATATCTTTCCCTAGGGTAAGGACAATGGTGGTGGTTATATTTGTGG
ATTGGGG
CATGCGGAAGAAGCACGAGAGCCACT
mm4_intergenic_4631405K08Rik|Plxna2_chr1_194565258 CAAGAGGAAGCACTGCCAGAATTATTGATCAAGATTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNTGAGCAGGGCTTGTTATTCATAAGGACATCTTCCCAGGGT
mm4_intergenic_Ndst4|Ugt8a_chr3_125827358 TCAGTTCCAGGCAGGGTACTACTTCTCAGTACCCCCTGCCAGCAACATGACCATCTCTCTGCCAGCCACAAGCTCACCAC
AGCTGCCTCCTGGTGCTCCCAGCTGCTGCAAGACAACCCCTGCCAGTC
mm4_intron_Gm5483_chr16_36185827 GGGAGGCAGAGAAACAGACAGTACTTATAGGAAGCATTTTCTGTATCACAGCAATAGTCCCCCCTCCAGAGAAAAAACCA
T
GGGGCACATATTATGGAAATCCC
mm4_intron_Rab31_chr17_65684069 ACAGCAGGCTTCTCCTTTCTTCTTTTAGAGTTATTTTTCTTGATGGTGGTGGTGCATGCCTGCTNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNAGATTAAATGCTGGGCCTCAAATATGCT
mm4_intron_Golga7b_chr19_42249897 GATGAAGTGCCGTGCTTGTGTCGGGGTGACAACACCCCATGCTCATCTTTCTGGCTGGTGGGTAGTATATATTCACTCTG
CTGACTGAAGGAACTGACGA
mm4_intron_Rad18_chr6_112677905 AGCAGGTCACAAGATACCGATTAATGGCTTAGAAAGTCATCTTGGGATATCTCCTTGTATTATCCCAAATCCCCGCCCCC
TAACTAGCAACATAACCAT
CTAAATACGATTCCCATCAACAAACTGTGGGT
mm4_intron_Sdk2_chr11_113844983 AAAGTACCCACCTTCCTGCCCAAAGGCTCACAAGATGTTGAAGGTTCTGTCTCTGGCTGGTGGGTCCAATCATTGACAGG
TGATTTATTAATAAATGCTCTCTCATCAATTAATACATTGATGAATTTATGGTGCAGGGT
mm4_intergenic_Gm25255|Csmd1_chr8_15848596 ACACACAGCCTATTCACGAGATTCTACAATTTCTGCAGAGTGATTTTACATTTACAACACCAGTAACCAGAAACATAATC
AT
TAAAAAGAAACCTCATCTAGACAGTCGTGTGGTGAAACTGAGGC
mm4_exon_Lpgat1_chr1_191760054 CCAACAGCTGCTGGTTCTCAAGAAGCACCTTGAACATAATTACAGGAGCAGAGATCGAAAATGGATCGTTTTGTTTCCGG
AAGGGGG
CTTCCTCAGGAAGAGGCGAGAAACAAGCCA
mm4_intron_Atp6v0a1_chr11_101040348 CCACCACCACAAACAAACAAACAACCACCACCATCAAGAAACAAAACAAAAAAACAAACCCCAAAACTGACTTACA
mm4_intergenic_Fbxo15|Gm25453_chr18_85962005 TCCACCCAACATGTGAGGTTTATTTTTTTCATGGCACAGTTGCTTTTTCAATTTGAAATTGCATAATCTTTCAAAAAAAA
AACCCATCACCCAGAAACATAAAAATCCGTGTTTAAGTACAGGAGTCA
mm4_intergenic_Fam180a|Mtpn_chr6_35480753 ACAAAACACCTAAGGCGAGGTCATTTATCAAGAATGTAGGTTTAGTATGCTTATGTTTCTGGGGGTTGGTGGTGGAGTAA
ATAGAATGGGGAGCAGAATGGTTATCACTTTGTGAGCTCCATGGCT
mm4_intergenic_Mut|Gm6771_chr17_41225277 ACAGCAGATTAACAGCCCCATCTGATACAACTATGAAACACAATGTATGTATCTTAAGTCACAGAAATATTGTTATGTTT
ATGGTAGGTGG
AAATTCATCCTATACAGGTCCTAACTAAGGTCGCATAGGGCAAC
mm4_intron_Zfp318_chr17_46389028 AGGTTGTAGCGCCATTAGCATATTCGTTATGTTTCCTGATGGTGGGTCTAGTCTTCGGCTAATTGAATAGACCA
mm4_exon_Mpp6_chr6_50110998 GTGGATTGGTTGGGGTGTCTTTGGTTATTTTTCTTGCTGGGGGGACTCTTTTTTTTTTTTTTTAACACTTTATTGGGAGT
TTATTCTCAAGGTACCAGTTCCTGGGATCGCAGCTCGAGAAAACATTCA
mm4_intron_Slc26a7_chr4_14601018 TGGCACAGGGTCTTAAGTATGAGACAAGTGTTATGACCATATCTGTGATTCTGGAGGATTCCAACTTGAACTTTCAGTAG
CTCCTTGTCCTCCAACCAGAAAAATAAAAATATCCCAGCCAGGAGACTGCATCTT
mm4_intron_Calcrl_chr2_84373421 TTCACGCCTTCTTCCGACTCTGCTGAGATGAGAGCCTGAGGGAATAAAACAACAACGGCTTTAGAATGGTGGAATGGTTA
GATTTCTTGCTGGTGG
AAAACAGCAAGAGCAGAGGTGC
mm4_intergenic_Gm22254|Gm8993_chr6_74803402 GCCCTACAGGAAACATTGCCATTATCTGTATTTCTTTTTATATTGTATATGTATGCCTGTGGACATGTTTATGTGTATGG
GTGGTGG
TGGTGGTAGATATATGTATATCAATACAAGTGCCAGCA
mm4_intron_Pip5k1b_chr19_24344215 CTTACCCACCCACCATCACCACCACCACCAAGAACCACAACCATTACAAAACCCCAACCCCAAATACCA
mm4_intergenic_Rbms3|Gm26614_chr9_117824180 CGGCTTGAACAGAATTCGGTTGGCTTTTTGAGTAGGAGATGTCTCTCTATTCAAAAGACTATGCTACTACACCACCAACC
ACCACCATAACCAT
AACCAAGACCATGGCTATCATTCATCCTGTATGGCTACTCCCTCCT
mm4_intron_Tenm4_chr7_96844135 CCTGGACATCCCCTGTTGAGTAGTTTCCAGTGTCAGTCCCCAATAAAAAAACATAACCATTTCTGAATTCTCACTGTACA
GAAAGCTCACTCCTTCTCAGAGATTCAACGCCTGTCTCCTGTC
mm4_intron_Mapre2_chr18_23872163 AGGAGAGGGAGGAACAGTCCCTCAGAGTAAGGAAGAGGTCAATGGCTTTGTGTCGGGATGGGGGATGCGTGTGCCCATAT
ACTTAACAC
mm4_intron_Gpd2_chr2_57345502 GCAACTTGCTGGGCCTTTCTTGCCTCCAGCCCAAAACATAACCACACCTTGTATACTATAACCCTCAGCCTTTTCCTCCC
CAC
mm4_intron_Gm5432_chr12_15861906 GGAGTCAGGCAACAGCAGATTAAAATTTCCATTTTGTGCTCTTGTGCTATAATTTTAAACCTAATATACTCCAAGAAAAG
TCCTACTGGAAGGTTATGTTTCTGCAAAGAGGTAGATCCCTTTTCCCTATCCTTGGCC
mm4_intron_Fyn_chr10_39470707 GTGCATACACAGCATACGTGTTGACGCGGTCCCACATACAAAAACATAACCTTTTTTTTGGTACACAACACAAGAAAGAA
AAAAATCCAGCCACCCACTCCAGTGTGCTTCAGTCCTGCTTTGGT
mm4_intergenic_Pitx1|H2afy_chr13_56015700 AGGAGAATTGAGAGAGACTGTCATATACAGGAAAATGGGTATGTGTGTGTATGGGGGGTGATTACCACTGAATGCAGCTA
AAACAACCAAGTTCAAATCCAGCTCAGTGTAAACCAGAC
mm4_intergenic_Mtpn|Gm23273_chr6_35785808 CCAGATGACAAGGGAGCCTCTTGTGATTGGTGAGTTTGGCTCACTTTTAGAGAAAAAAAATTTTTTTTTGGTACAAATTA
TGGTTTTGTTTCTGTTTTGTGG
GAGTTACTTTCTTTCTGAAATTGTGTGTATTTTCACCC
mm4_intergenic_Dscaml1|Cep164_chr9_45753697 CCAAGAAAACCGGGAGTTGCTTCAATTTGCAGATGCTTATGTGCTAATACCTTTTTCTATGAAAAAAGACCCAGCGCCGT
GTGCAATAAAGGTTATGTTTCTACGTGGTGGCTTTTTTCCCATCTGGCAAGGGC
mm4_intergenic_Cyp2c37|Cyp2c54_chr19_40013158 ACAGATTTAAAATCAGACTTGCCATATCTCTATTACTATTACCAACTGACCATCATACAGAAACAAAACCAGACAAAAGA
ACATTCAATCAAGATACTTTGAACTCAATATCAAAGCCAGCAACAAAGGT
mm4_intergenic_4931417E11Rik|Gm26179_chr6_73631806 GCTTAAAGGAGGAGAGAGCTGAAAATATTTTAACAAAGAAGACTACAAATTAAGAGATATTAACAGAGGAGTGGTTTTGT
TTCTCTATGGAGG
TGTCAGAGCTTCGGGACAGTT
mm4_intergenic_1700112E06Rik|Gm10248_chr14_22915664 AGAGCACTGGCAAACCTTGAACAAGGTGCTAGAGTGGTGATGTTTCGGCATGGGGGCACATGGAAGCTGTGTGCATCTCA
GCTCTACTTGAGCCAAGGAAGCGTTAGG

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_mN1FkRligAThyqKv3eg3.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.