CRISPOR

DNA break repair outcome predictions

Guide: GAGATGGCAGTGGGGAGCTA GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CCGTGTCGACGGCGACCGCGGAGATGGCAGTGGGGAGCTAGGGTCACCTTGTGATGCGAGCGCTCCTCTGTGCCGCACCG`	(Wild-type sequence)
402	`GGCGACCGCGGAGATGGCAGTGGGG--------TCACCTTGTGATGCGAGCGCTCCTCTG`	`8 bp deleted → frameshift`
382	`GGCGACCGCGGAGATGGCAGTGGG---------TCACCTTGTGATGCGAGCGCTCCTCTG`	`9 bp deleted → no frameshift`
282	`GGCGACCGCGGAGATGGCAGTGGGG-------GTCACCTTGTGATGCGAGCGCTCCTCTG`	`7 bp deleted → frameshift`
242	`GGCGACCGCGGAGATGGCAGTGG----------TCACCTTGTGATGCGAGCGCTCCTCTG`	`10 bp deleted → frameshift`
221	`GGCGACCGCGGAGATGGCAGTGGGGAGC-----------------------GCTCCTCTG`	`23 bp deleted → frameshift`
188	`GGCGACCGCGGAGATGG---------------GTCACCTTGTGATGCGAGCGCTCCTCTG`	`15 bp deleted → no frameshift`
182	`GGCGACCGCGGAGATGGCAGTGGGGAGCT----------TGTGATGCGAGCGCTCCTCTG`	`10 bp deleted → frameshift`
179	`GGCGACCGCGGAGATGG----------------TCACCTTGTGATGCGAGCGCTCCTCTG`	`16 bp deleted → frameshift`
175	`GGCGACCGCGGAGATGGCAGTG---------------------ATGCGAGCGCTCCTCTG`	`21 bp deleted → no frameshift`
156	`GGCGACCGCGGAGATGGCAGT-------------CACCTTGTGATGCGAGCGCTCCTCTG`	`13 bp deleted → frameshift`
154	`GGCGACCGCGGAGATGGCAGTGGGGAGC-------------------GAGCGCTCCTCTG`	`19 bp deleted → frameshift`
143	`GGCGACCGCGGAGATGGCAGTGGGGAGCT-------------------------CCTCTG`	`25 bp deleted → frameshift`
140	`GGCGACCGCGG---------------------GTCACCTTGTGATGCGAGCGCTCCTCTG`	`21 bp deleted → no frameshift`
133	`GGCGACCGCGGAGATG------------------------------CGAGCGCTCCTCTG`	`30 bp deleted → no frameshift`
133	`GGCGACCGCGG----------------------TCACCTTGTGATGCGAGCGCTCCTCTG`	`22 bp deleted → frameshift`
128	`GGCGACCGCGGAGATGGCA-----------------CCTTGTGATGCGAGCGCTCCTCTG`	`17 bp deleted → frameshift`
122	`GGCGACCGCGGAGATGGCAGTGGGGA------------------TGCGAGCGCTCCTCTG`	`18 bp deleted → no frameshift`
116	`GGCGACCGCGGAGATGGCAGTG-------------------TGATGCGAGCGCTCCTCTG`	`19 bp deleted → frameshift`
106	`GGCGACC-------------------------------TTGTGATGCGAGCGCTCCTCTG`	`31 bp deleted → frameshift`
94	`GGCGACCGCGGAGATGGC-----------------------------GAGCGCTCCTCTG`	`29 bp deleted → frameshift`
90	`GGCGACCGCGGAGATGGCAGTG------------------------CGAGCGCTCCTCTG`	`24 bp deleted → no frameshift`
90	`GGCGACCGCG--------------------------------------AGCGCTCCTCTG`	`38 bp deleted → frameshift`
89	`GG------------------------------GTCACCTTGTGATGCGAGCGCTCCTCTG`	`30 bp deleted → no frameshift`
86	`GGCGACCGCGGAGATG-------------------------TGATGCGAGCGCTCCTCTG`	`25 bp deleted → frameshift`
84	`GG-------------------------------TCACCTTGTGATGCGAGCGCTCCTCTG`	`31 bp deleted → frameshift`
78	`GGCGACCGCGGAG-------------------------------------CGCTCCTCTG`	`37 bp deleted → frameshift`
77	`GGCGACCGCGGAGATG---------------------------ATGCGAGCGCTCCTCTG`	`27 bp deleted → no frameshift`
77	`GGCGA--------------------------------------------GCGCTCCTCTG`	`44 bp deleted → frameshift`
76	`GGCGACCGCGGAGATGGC---------------------------------GCTCCTCTG`	`33 bp deleted → no frameshift`
74	`GGCGACCGCGGAGATGGCAGTGGGGAGCT----------------------------CTG`	`28 bp deleted → frameshift`
73	`GGCGACCGCG------------------------------------------CTCCTCTG`	`42 bp deleted → no frameshift`
69	`GGCGACCGCGGAGATGGC-----------------------------------TCCTCTG`	`35 bp deleted → frameshift`
66	`GGCGACCGCGGAGATGGCAGTGGGGAGCT------------------------------G`	`30 bp deleted → no frameshift`
66	`GGCGACCGCGGAGATGGCAG------------------------------CGCTCCTCTG`	`30 bp deleted → no frameshift`
66	`GGCGACCGC--------------------------------------------TCCTCTG`	`44 bp deleted → frameshift`
60	`GGCGACCGCGGA--------------------------------TGCGAGCGCTCCTCTG`	`32 bp deleted → frameshift`
54	`GGCG------------------------------------------------CTCCTCTG`	`48 bp deleted → no frameshift`
54	`GGCGACCG----------------------------------------AGCGCTCCTCTG`	`40 bp deleted → frameshift`
52	`GGCGACCGCGGAGA-----------------------------------GCGCTCCTCTG`	`35 bp deleted → frameshift`
45	`GGCGACCGCGGAGATGGCAGTG--------------------------------------`	`38 bp deleted → frameshift`
42	`GGCGA---------------------------------------TGCGAGCGCTCCTCTG`	`39 bp deleted → no frameshift`
34	`GGCGACC-------------------------------------------------TCTG`	`49 bp deleted → frameshift`
33	`GGCGACCGCGGAGATG--------------------------------------------`	`44 bp deleted → frameshift`
32	`GGC--------------------------------------------------TCCTCTG`	`50 bp deleted → frameshift`

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