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Contents:

PCR primers for off-targets of TCCCCCCCTCTGGAGAGTCC GGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313820_F TCGTCGGCAGCGTCAGGGAGCCTGGGATTAGGAG 60.1 1.00
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313820_R GTCTCGTGGGCTCGGCATGTCCTTTCGGCTCTCCA 59.7 1.00
mm4_intron_Adap1_chr5_139287250_F Primer3: not found at this Tm N.d. 0.69
mm4_intron_Adap1_chr5_139287250_R Primer3: not found at this Tm N.d. 0.69
mm4_exon_Zbtb39/Gpr182_chr10_127742015_F Primer3: not found at this Tm N.d. 0.62
mm4_exon_Zbtb39/Gpr182_chr10_127742015_R Primer3: not found at this Tm N.d. 0.62
mm3_intron_Sufu_chr19_46477777_F TCGTCGGCAGCGTCAGTTGCTGTGCTAGGAGCTG 60.0 0.60
mm3_intron_Sufu_chr19_46477777_R GTCTCGTGGGCTCGGCTTGGTGATCAAGGGTGCGT 60.6 0.60
mm4_intergenic_Gm21614|Gm23249_chr12_93537839_F TCGTCGGCAGCGTCACACAGCTCTTGTTTCTGACA 57.7 0.52
mm4_intergenic_Gm21614|Gm23249_chr12_93537839_R GTCTCGTGGGCTCGGACTGAATTTAACAGCACTTTCCACT 59.4 0.52
mm4_intron_Acpp_chr9_104310397_F TCGTCGGCAGCGTCTGGTCAGCCAGCCTTGTTAG 59.9 0.50
mm4_intron_Acpp_chr9_104310397_R GTCTCGTGGGCTCGGAGACAAACTGGAAGTGGCAGT 59.7 0.50
mm4_intron_Krt79_chr15_101931687_F Primer3: not found at this Tm N.d. 0.50
mm4_intron_Krt79_chr15_101931687_R Primer3: not found at this Tm N.d. 0.50
mm4_intron_Tmem150c_chr5_100145502_F Primer3: not found at this Tm N.d. 0.47
mm4_intron_Tmem150c_chr5_100145502_R Primer3: not found at this Tm N.d. 0.47
mm4_intergenic_Mcf2|Atp11c_chrX_60211239_F Primer3: not found at this Tm N.d. 0.46
mm4_intergenic_Mcf2|Atp11c_chrX_60211239_R Primer3: not found at this Tm N.d. 0.46
mm4_intergenic_4930564D02Rik|1700095B22Rik_chr3_105317211_F Primer3: not found at this Tm N.d. 0.46
mm4_intergenic_4930564D02Rik|1700095B22Rik_chr3_105317211_R Primer3: not found at this Tm N.d. 0.46
mm4_intron_Tesc_chr5_118039473_F Primer3: not found at this Tm N.d. 0.45
mm4_intron_Tesc_chr5_118039473_R Primer3: not found at this Tm N.d. 0.45
mm4_intron_Pard3_chr8_127154993_F Primer3: not found at this Tm N.d. 0.45
mm4_intron_Pard3_chr8_127154993_R Primer3: not found at this Tm N.d. 0.45
mm4_intergenic_Igfbp5|Tnp1_chr1_72877283_F TCGTCGGCAGCGTCAGTCCATTCTCTCTGCCCTCT 59.9 0.42
mm4_intergenic_Igfbp5|Tnp1_chr1_72877283_R GTCTCGTGGGCTCGGTCTCTGAGAGTGCGACCAGA 59.9 0.42
mm4_intergenic_Otol1|Gm23477_chr3_70052788_F Primer3: not found at this Tm N.d. 0.42
mm4_intergenic_Otol1|Gm23477_chr3_70052788_R Primer3: not found at this Tm N.d. 0.42
mm4_intergenic_Gm13503|Gm13502_chr2_53633265_F Primer3: not found at this Tm N.d. 0.42
mm4_intergenic_Gm13503|Gm13502_chr2_53633265_R Primer3: not found at this Tm N.d. 0.42
mm3_intergenic_BC017158|Rgs10_chr7_128307284_F Primer3: not found at this Tm N.d. 0.41
mm3_intergenic_BC017158|Rgs10_chr7_128307284_R Primer3: not found at this Tm N.d. 0.41
mm4_exon_Krba1_chr6_48416009_F Primer3: not found at this Tm N.d. 0.40
mm4_exon_Krba1_chr6_48416009_R Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Gm24291|Mon1b_chr8_113551666_F TCGTCGGCAGCGTCGCGTTGTTCTGAACCGGAAG 59.7 0.40
mm4_intergenic_Gm24291|Mon1b_chr8_113551666_R GTCTCGTGGGCTCGGCCTCTCCTTCCTCCCTTGGA 59.9 0.40
mm4_intergenic_Zfp358|Mcoln1_chr8_3500010_F TCGTCGGCAGCGTCTGCAAAGCCTTGGGAAGTCA 60.1 0.36
mm4_intergenic_Zfp358|Mcoln1_chr8_3500010_R GTCTCGTGGGCTCGGAGACTTTCTGCGCTACTGCA 59.6 0.36
mm4_intron_Disc1_chr8_125204844_F Primer3: not found at this Tm N.d. 0.36
mm4_intron_Disc1_chr8_125204844_R Primer3: not found at this Tm N.d. 0.36
mm3_exon_Tmem233_chr5_116040632_F TCGTCGGCAGCGTCTCCCGACTAGTGGGTGTTCA 60.1 0.34
mm3_exon_Tmem233_chr5_116040632_R GTCTCGTGGGCTCGGCTCATCTGCACACACTCCCA 59.6 0.34
mm4_intergenic_Gm24587|Dcn_chr10_96925839_F Primer3: not found at this Tm N.d. 0.32
mm4_intergenic_Gm24587|Dcn_chr10_96925839_R Primer3: not found at this Tm N.d. 0.32
mm4_intergenic_Gm24696|Myc_chr15_61984264_F TCGTCGGCAGCGTCTTCTAGGGTCTCTGCAGGCT 59.9 0.31
mm4_intergenic_Gm24696|Myc_chr15_61984264_R GTCTCGTGGGCTCGGTGGGGTGGGGAAAATCAAGG 59.8 0.31
mm3_intergenic_Ajap1|Gm25880_chr4_153683070_F TCGTCGGCAGCGTCAGAAATGCTGGGCACACAGA 59.8 0.30
mm3_intergenic_Ajap1|Gm25880_chr4_153683070_R GTCTCGTGGGCTCGGGTCCATCTGCTTGCATGAGC 59.6 0.30
mm4_intergenic_Dlk1|Mico1_chr12_109489422_F TCGTCGGCAGCGTCTGTCCTCTCAAAGTCTTGGCT 58.9 0.30
mm4_intergenic_Dlk1|Mico1_chr12_109489422_R GTCTCGTGGGCTCGGTCCCGATTTCCACTGTCTGA 58.3 0.30
mm4_intergenic_1700112E06Rik|Gm5670_chr14_22534681_F Primer3: not found at this Tm N.d. 0.30
mm4_intergenic_1700112E06Rik|Gm5670_chr14_22534681_R Primer3: not found at this Tm N.d. 0.30
mm3_exon_Nynrin/Gm26751_chr14_55871429_F TCGTCGGCAGCGTCTGCAACTGGATCTTCAGCGT 59.9 0.29
mm3_exon_Nynrin/Gm26751_chr14_55871429_R GTCTCGTGGGCTCGGGATGAGGTAGTGAAGCCCCG 59.8 0.29
mm4_intergenic_Etv4|Meox1_chr11_101787038_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Etv4|Meox1_chr11_101787038_R Primer3: not found at this Tm N.d. 0.27
mm3_intergenic_Rora|F830001A07Rik_chr9_68823587_F Primer3: not found at this Tm N.d. 0.26
mm3_intergenic_Rora|F830001A07Rik_chr9_68823587_R Primer3: not found at this Tm N.d. 0.26
mm4_intron_Stra6_chr9_58129211_F TCGTCGGCAGCGTCTGGGGAAACTCAGCCATCAA 59.2 0.25
mm4_intron_Stra6_chr9_58129211_R GTCTCGTGGGCTCGGAACCGTTTGCCCGAAACTTG 59.8 0.25
mm4_intron_Theg_chr10_79581565_F TCGTCGGCAGCGTCCCGTCAGCTGTTACAGGGAG 60.1 0.24
mm4_intron_Theg_chr10_79581565_R GTCTCGTGGGCTCGGTACAGCTGCCATGGTCACTG 60.0 0.24
mm4_intergenic_Gm5091|Dll1_chr17_15333718_F TCGTCGGCAGCGTCGCAAAGCCAGTCTTTTGTGGA 59.5 0.23
mm4_intergenic_Gm5091|Dll1_chr17_15333718_R GTCTCGTGGGCTCGGACAGCCCGATGGAGATTTCA 59.0 0.23
mm4_intergenic_Gm24120|Nr2f2_chr7_70350082_F TCGTCGGCAGCGTCTTTGGAACTGGACAGGGACG 59.8 0.23
mm4_intergenic_Gm24120|Nr2f2_chr7_70350082_R GTCTCGTGGGCTCGGGACTGGCAGGAGCACAGATT 60.0 0.23
mm4_intergenic_Il23a|Gm23241_chr10_128298200_F TCGTCGGCAGCGTCCAGGTGACTCAGAGCAGGTG 60.0 0.22
mm4_intergenic_Il23a|Gm23241_chr10_128298200_R GTCTCGTGGGCTCGGGCCTCTAGCCACAACAACCT 59.9 0.22
mm4_intergenic_4930552P12Rik|Tcf7l2_chr19_55733085_F TCGTCGGCAGCGTCGGAGAGGAACAGGGTGAGGA 60.2 0.22
mm4_intergenic_4930552P12Rik|Tcf7l2_chr19_55733085_R GTCTCGTGGGCTCGGGCTGTCGCTTTTCCTAGGGT 60.0 0.22
mm3_intron_Sorl1_chr9_41973184_F Primer3: not found at this Tm N.d. 0.22
mm3_intron_Sorl1_chr9_41973184_R Primer3: not found at this Tm N.d. 0.22
mm4_intron_Tnfrsf21_chr17_43040237_F TCGTCGGCAGCGTCACGGCCATGGTGAGTTTCTT 59.8 0.22
mm4_intron_Tnfrsf21_chr17_43040237_R GTCTCGTGGGCTCGGGGTGAGGAAAAGGGACTCCA 58.9 0.22
mm4_intergenic_D3Bwg0562e|4833424O15Rik_chr3_117523850_F TCGTCGGCAGCGTCCTTCCTCAGAACCACCGGAC 60.0 0.21
mm4_intergenic_D3Bwg0562e|4833424O15Rik_chr3_117523850_R GTCTCGTGGGCTCGGGGGGAGACTCCTCATGCTCT 60.3 0.21
mm4_intergenic_Gm8730|Cdh5_chr8_103672675_F Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Gm8730|Cdh5_chr8_103672675_R Primer3: not found at this Tm N.d. 0.21
mm4_intron_Pik3c2a_chr7_116349932_F Primer3: not found at this Tm N.d. 0.21
mm4_intron_Pik3c2a_chr7_116349932_R Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Gm12379|Gm12380_chr4_38214586_F Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Gm12379|Gm12380_chr4_38214586_R Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Dytn|Gm12748_chr1_63666867_F TCGTCGGCAGCGTCTCATGCAGTCTGGCCTCAAA 59.5 0.20
mm4_intergenic_Dytn|Gm12748_chr1_63666867_R GTCTCGTGGGCTCGGTGCAGTTTTGTGTGCATGTGT 59.7 0.20
mm4_intron_Ryr2_chr13_11742770_F TCGTCGGCAGCGTCGGTCCTGACAACCTACCTGC 60.0 0.19
mm4_intron_Ryr2_chr13_11742770_R GTCTCGTGGGCTCGGGGAGAACTCCTGTAGAAAGCAGT 59.7 0.19
mm4_intergenic_Vmn1r62|Vmn1r63_chr7_5788763_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Vmn1r62|Vmn1r63_chr7_5788763_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_VMN1R62|VMN1R63_chr7_GL456219_random_135297_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_VMN1R62|VMN1R63_chr7_GL456219_random_135297_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Spata19|Opcml_chr9_27684840_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Spata19|Opcml_chr9_27684840_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Pcdh20|Gm25886_chr14_88696092_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Pcdh20|Gm25886_chr14_88696092_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Maf/Gm15655|Dynlrb2_chr8_116497863_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Maf/Gm15655|Dynlrb2_chr8_116497863_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm4929|Fam84a_chr12_13917586_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm4929|Fam84a_chr12_13917586_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm25255|Csmd1_chr8_15641623_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm25255|Csmd1_chr8_15641623_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm23389|Gm23181_chr1_94921770_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm23389|Gm23181_chr1_94921770_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm23276|Dach1_chr14_97723587_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm23276|Dach1_chr14_97723587_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm22815|Cntn5_chr9_9403421_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm22815|Cntn5_chr9_9403421_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm14894|Gm5942_chrX_114679118_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm14894|Gm5942_chrX_114679118_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm13916|Gm13915_chr2_108489610_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm13916|Gm13915_chr2_108489610_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Fam173b|Tas2r119_chr15_31894005_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Fam173b|Tas2r119_chr15_31894005_R Primer3: not found at this Tm N.d. 0.19
mm4_intron_Dap_chr15_31244035_F Primer3: not found at this Tm N.d. 0.19
mm4_intron_Dap_chr15_31244035_R Primer3: not found at this Tm N.d. 0.19
mm3_intron_Plekha7_chr7_116288628_F TCGTCGGCAGCGTCGGGATGACTACCTGGGGACT 60.0 0.18
mm3_intron_Plekha7_chr7_116288628_R GTCTCGTGGGCTCGGGCCAGCAACTTCCTCTCACT 59.9 0.18
mm4_exon_Fbxl2_chr9_113984093_F TCGTCGGCAGCGTCCACTAGGGCTTAACACCCGG 60.1 0.18
mm4_exon_Fbxl2_chr9_113984093_R GTCTCGTGGGCTCGGAGGAGTTGACAGTGTGAGCC 59.6 0.18
mm4_intron_Ccdc125_chr13_100690887_F Primer3: not found at this Tm N.d. 0.18
mm4_intron_Ccdc125_chr13_100690887_R Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_C430002E04Rik|Phf17_chr3_41498945_F TCGTCGGCAGCGTCGCATGGCCTAGCACAGATCT 59.8 0.18
mm4_intergenic_C430002E04Rik|Phf17_chr3_41498945_R GTCTCGTGGGCTCGGCCAGTGTATCGCCAGCTGAA 60.1 0.18
mm4_intergenic_Gm4994|Gm7058_chrX_131962250_F Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Gm4994|Gm7058_chrX_131962250_R Primer3: not found at this Tm N.d. 0.18
mm4_intron_Dgat2_chr7_99176682_F TCGTCGGCAGCGTCAGGACCAAGCTCAGAGGGAT 59.9 0.17
mm4_intron_Dgat2_chr7_99176682_R GTCTCGTGGGCTCGGCAGGGCCCATGACTTCCTTA 59.0 0.17
mm4_intergenic_Macrod2|Rpl26-ps5_chr2_142548156_F Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Macrod2|Rpl26-ps5_chr2_142548156_R Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Gm26651|Diras2_chr13_52457254_F TCGTCGGCAGCGTCCCGTAAATGGGTACAAATTCTTCT 57.2 0.17
mm4_intergenic_Gm26651|Diras2_chr13_52457254_R GTCTCGTGGGCTCGGTGCCTAGTTTACTCAGAAGGTAGA 58.4 0.17
mm4_intergenic_Sppl2c|Mapt_chr11_104196716_F TCGTCGGCAGCGTCCCCACCCACAAGACTTGACT 59.5 0.17
mm4_intergenic_Sppl2c|Mapt_chr11_104196716_R GTCTCGTGGGCTCGGTTGGGCAAGCTGCTTTCTCT 60.1 0.17
mm4_intergenic_Gm16477|Gpr26_chr7_131728218_F TCGTCGGCAGCGTCTCATGAACCTGGAAGGCTGG 59.6 0.16
mm4_intergenic_Gm16477|Gpr26_chr7_131728218_R GTCTCGTGGGCTCGGTTAGCTCCCACTAAGCCAGC 59.4 0.16
mm4_intron_Hps4_chr5_112364958_F TCGTCGGCAGCGTCGCATCCGTACTCACTGCCAA 60.3 0.16
mm4_intron_Hps4_chr5_112364958_R GTCTCGTGGGCTCGGCAGGATGGCAGAGCAGAGAG 59.8 0.16
mm4_intron_Gsk3b_chr16_38182225_F TCGTCGGCAGCGTCTGCTTTGTTAAACTGGCTGCC 59.9 0.16
mm4_intron_Gsk3b_chr16_38182225_R GTCTCGTGGGCTCGGGTGCTGTGTGTGCTGTGTTT 59.8 0.16
mm3_intergenic_Ifne|Mir31_chr4_88907333_F Primer3: not found at this Tm N.d. 0.15
mm3_intergenic_Ifne|Mir31_chr4_88907333_R Primer3: not found at this Tm N.d. 0.15
mm4_intron_Rnls_chr19_33369969_F TCGTCGGCAGCGTCGCAGTGGGCATCTCTTCTGT 60.0 0.15
mm4_intron_Rnls_chr19_33369969_R GTCTCGTGGGCTCGGTGCAAAACAGCAAATTTGGATACA 58.6 0.15
mm4_intergenic_Mir29b-1/Gm27019|Gm13834_chr6_31068371_F TCGTCGGCAGCGTCAGCGAGGCTCACAATGTACC 60.1 0.15
mm4_intergenic_Mir29b-1/Gm27019|Gm13834_chr6_31068371_R GTCTCGTGGGCTCGGCGCAGGCTGATACTCCTGAG 59.9 0.15
mm4_intron_Prkcq_chr2_11200398_F Primer3: not found at this Tm N.d. 0.15
mm4_intron_Prkcq_chr2_11200398_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Slc22a23|Pxdc1_chr13_34568903_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Slc22a23|Pxdc1_chr13_34568903_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm24999|Gm25000_chr13_60312840_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Gm24999|Gm25000_chr13_60312840_R Primer3: not found at this Tm N.d. 0.14
mm4_intron_Rtn1_chr12_72298389_F Primer3: not found at this Tm N.d. 0.14
mm4_intron_Rtn1_chr12_72298389_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Vmn1r-ps54|Vmn1r74_chr7_11803440_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Vmn1r-ps54|Vmn1r74_chr7_11803440_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Dctd|Tenm3_chr8_48167551_F TCGTCGGCAGCGTCAATGTGTGCCAGGACCTGAC 60.2 0.14
mm4_intergenic_Dctd|Tenm3_chr8_48167551_R GTCTCGTGGGCTCGGTTCTGGAGGGAGGGAGATGG 60.0 0.14
mm4_intergenic_Csmd1|Gm25665_chr8_18051950_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Csmd1|Gm25665_chr8_18051950_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Skor1|Map2k5_chr9_63158769_F TCGTCGGCAGCGTCCCCGCCTCTCTTTTCCATGT 60.0 0.14
mm4_intergenic_Skor1|Map2k5_chr9_63158769_R GTCTCGTGGGCTCGGTGCTGGGCTCTCCTAGGAAT 60.0 0.14
mm4_intron_Rbms3_chr9_116711545_F TCGTCGGCAGCGTCTCCATCCTGGGAGATTCCTGT 59.9 0.13
mm4_intron_Rbms3_chr9_116711545_R GTCTCGTGGGCTCGGCTGGTCAACCCTAGAGCTGC 60.1 0.13
mm3_intergenic_Gm22788|Gm24432_chr18_34064847_F Primer3: not found at this Tm N.d. 0.13
mm3_intergenic_Gm22788|Gm24432_chr18_34064847_R Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Gm27034|Rpl36a-ps1_chr14_98845936_F TCGTCGGCAGCGTCTGATGGAAGGCTGAGTGCTG 60.0 0.13
mm4_intergenic_Gm27034|Rpl36a-ps1_chr14_98845936_R GTCTCGTGGGCTCGGAGGCATCGCAAAAGGTGAGA 59.9 0.13
mm3_intergenic_Gm16079|Gm13049_chr4_150780826_F TCGTCGGCAGCGTCCCCTGGGTTCTTTCGACCTC 60.0 0.13
mm3_intergenic_Gm16079|Gm13049_chr4_150780826_R GTCTCGTGGGCTCGGACCTGCTGCAGACCTTTTCA 59.8 0.13
mm3_intergenic_1700013D24Rik|Pex5_chr6_124392547_F Primer3: not found at this Tm N.d. 0.12
mm3_intergenic_1700013D24Rik|Pex5_chr6_124392547_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Catsper3|Pitx1_chr13_55816202_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Catsper3|Pitx1_chr13_55816202_R Primer3: not found at this Tm N.d. 0.12
mm4_exon_Hsd17b12_chr2_94114746_F Primer3: not found at this Tm N.d. 0.11
mm4_exon_Hsd17b12_chr2_94114746_R Primer3: not found at this Tm N.d. 0.11
mm3_intron_Fads2_chr19_10100899_F TCGTCGGCAGCGTCGGCCATCTTCCACTTGGGG 60.3 0.11
mm3_intron_Fads2_chr19_10100899_R GTCTCGTGGGCTCGGTGCATGTTCACAGCGCATAC 59.5 0.11
mm4_intergenic_Aplp2|Gm7244_chr9_31212777_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Aplp2|Gm7244_chr9_31212777_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Hmg20a|Gm26868_chr9_56547332_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Hmg20a|Gm26868_chr9_56547332_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Rora|F830001A07Rik_chr9_68679438_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Rora|F830001A07Rik_chr9_68679438_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Clrn1|Gm22491_chr3_58908667_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Clrn1|Gm22491_chr3_58908667_R Primer3: not found at this Tm N.d. 0.11
mm4_intron_Rbms3_chr9_117176131_F Primer3: not found at this Tm N.d. 0.11
mm4_intron_Rbms3_chr9_117176131_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm22492|Pdcd6ip_chr9_113455155_F TCGTCGGCAGCGTCTCTGCTCTCAAAACAGCCCA 59.5 0.11
mm4_intergenic_Gm22492|Pdcd6ip_chr9_113455155_R GTCTCGTGGGCTCGGATCTCTGCTCCACGGTCTCT 60.0 0.11
mm4_exon_Scap_chr9_110381343_F Primer3: not found at this Tm N.d. 0.10
mm4_exon_Scap_chr9_110381343_R Primer3: not found at this Tm N.d. 0.10
mm4_exon_Sncaip_chr18_52768446_F TCGTCGGCAGCGTCTCTGTCCGTCCATCCCTCC 60.3 0.10
mm4_exon_Sncaip_chr18_52768446_R GTCTCGTGGGCTCGGGGTCCCGAAAACCCTCAGG 60.0 0.10
mm4_intron_Fgf1_chr18_38861062_F Primer3: not found at this Tm N.d. 0.10
mm4_intron_Fgf1_chr18_38861062_R Primer3: not found at this Tm N.d. 0.10
mm4_intron_Nhsl1_chr10_18392272_F Primer3: not found at this Tm N.d. 0.10
mm4_intron_Nhsl1_chr10_18392272_R Primer3: not found at this Tm N.d. 0.10
mm4_intron_Lama3_chr18_12359154_F Primer3: not found at this Tm N.d. 0.10
mm4_intron_Lama3_chr18_12359154_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Six2|Srbd1_chr17_85696625_F TCGTCGGCAGCGTCGTCCCTGTTTGCCTCTGGAA 59.8 0.09
mm4_intergenic_Six2|Srbd1_chr17_85696625_R GTCTCGTGGGCTCGGAAGAACTCAGTGGGGCCATG 59.9 0.09
mm4_exon_Ankrd33_chr15_101119627_F TCGTCGGCAGCGTCGCACGGTATGAGCTGACACT 60.1 0.09
mm4_exon_Ankrd33_chr15_101119627_R GTCTCGTGGGCTCGGCACCTCCTCGTGGTTCTGTT 59.6 0.09
mm4_intergenic_Gm5054|Ifitm10_chr7_142320863_F TCGTCGGCAGCGTCGCTGCTGTCCCCTCTATTCC 59.8 0.09
mm4_intergenic_Gm5054|Ifitm10_chr7_142320863_R GTCTCGTGGGCTCGGAGGGTAAGGTCATGGTCCCA 59.8 0.09
mm4_intergenic_1700001G01Rik|Gm26235_chr18_17290699_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_1700001G01Rik|Gm26235_chr18_17290699_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Aldh1a3|Asb7_chr7_66626678_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Aldh1a3|Asb7_chr7_66626678_R Primer3: not found at this Tm N.d. 0.09
mm3_exon_Nfatc1_chr18_80698421_F Primer3: not found at this Tm N.d. 0.09
mm3_exon_Nfatc1_chr18_80698421_R Primer3: not found at this Tm N.d. 0.09
mm4_intron_Ccdc85c_chr12_108245829_F TCGTCGGCAGCGTCGTGATTCGACCTCCCTGAGC 60.1 0.08
mm4_intron_Ccdc85c_chr12_108245829_R GTCTCGTGGGCTCGGATCGCAATGAAGGACACCGT 60.0 0.08
mm4_intergenic_Mir674|Rap1a-ps2_chr2_117200099_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Mir674|Rap1a-ps2_chr2_117200099_R Primer3: not found at this Tm N.d. 0.08
mm4_intron_Ext2_chr2_93700420_F Primer3: not found at this Tm N.d. 0.08
mm4_intron_Ext2_chr2_93700420_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_4930564D02Rik|1700095B22Rik_chr3_105331568_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_4930564D02Rik|1700095B22Rik_chr3_105331568_R Primer3: not found at this Tm N.d. 0.08
mm4_intron_Sdk2_chr11_113805850_F Primer3: not found at this Tm N.d. 0.08
mm4_intron_Sdk2_chr11_113805850_R Primer3: not found at this Tm N.d. 0.08
mm4_intron_Gm3054_chr5_137165146_F TCGTCGGCAGCGTCCGTAGCAGGGCAAGGTTGTA 60.0 0.08
mm4_intron_Gm3054_chr5_137165146_R GTCTCGTGGGCTCGGCCTCCTCCATCCTGAAGCTC 59.2 0.08
mm3_intergenic_3110083C13Rik|Fgf9_chr14_58036449_F TCGTCGGCAGCGTCTGGCGTTGAAAGGGTAGGAC 59.9 0.07
mm3_intergenic_3110083C13Rik|Fgf9_chr14_58036449_R GTCTCGTGGGCTCGGACAGAACCTTCCTGGAGGGA 59.8 0.07
mm4_exon_Cog1/Fam104a_chr11_113662215_F Primer3: not found at this Tm N.d. 0.07
mm4_exon_Cog1/Fam104a_chr11_113662215_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm10858|Gtf3a_chr5_146914068_F TCGTCGGCAGCGTCTTGTCTACACAGTGGCAGGC 60.2 0.07
mm4_intergenic_Gm10858|Gtf3a_chr5_146914068_R GTCTCGTGGGCTCGGAGGTTTCCCTTGCCAGAACC 60.1 0.07
mm4_intron_Aldh1a2_chr9_71236734_F TCGTCGGCAGCGTCTGTTGCCAAACAGCTGTGAC 59.5 0.07
mm4_intron_Aldh1a2_chr9_71236734_R GTCTCGTGGGCTCGGTCTGAGTGAGTGAAGTGCCT 58.2 0.07
mm4_intron_Tsku_chr7_98355068_F TCGTCGGCAGCGTCACAGCCCAGATGTTGCTCTC 60.0 0.07
mm4_intron_Tsku_chr7_98355068_R GTCTCGTGGGCTCGGCAGGGGTTTGTTGGCTTGTG 59.8 0.07
mm4_intergenic_Gm20424|Lbx1_chr19_45201003_F TCGTCGGCAGCGTCGGCATTTCAGACTGGGCTCT 60.0 0.07
mm4_intergenic_Gm20424|Lbx1_chr19_45201003_R GTCTCGTGGGCTCGGTTCCTCCTGCAAACCCCTTC 59.8 0.07
mm4_intergenic_Efnb1|Gm14812_chrX_99187257_F TCGTCGGCAGCGTCTGGCCCTCTCTGAGACATGA 59.9 0.07
mm4_intergenic_Efnb1|Gm14812_chrX_99187257_R GTCTCGTGGGCTCGGAAAGTGACAGGGCTGACCAG 59.8 0.07
mm4_intergenic_Gm12057|RP23-242C19.7/B3gnt2_chr11_22833557_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm12057|RP23-242C19.7/B3gnt2_chr11_22833557_R Primer3: not found at this Tm N.d. 0.06
mm3_intergenic_Mep1a|Ankrd66_chr17_43519026_F Primer3: not found at this Tm N.d. 0.06
mm3_intergenic_Mep1a|Ankrd66_chr17_43519026_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Igkv3-7|Igkv3-5_chr6_70620614_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Igkv3-7|Igkv3-5_chr6_70620614_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Mfsd7c_chr12_85778862_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Mfsd7c_chr12_85778862_R Primer3: not found at this Tm N.d. 0.06
mm3_intron_Znrf3_chr11_5426872_F Primer3: not found at this Tm N.d. 0.06
mm3_intron_Znrf3_chr11_5426872_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Zfand3_chr17_30115887_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Zfand3_chr17_30115887_R Primer3: not found at this Tm N.d. 0.06
mm3_intergenic_Kctd3|Kcnk2_chr1_189088958_F Primer3: not found at this Tm N.d. 0.06
mm3_intergenic_Kctd3|Kcnk2_chr1_189088958_R Primer3: not found at this Tm N.d. 0.06
mm4_exon_Meis2_chr2_116049118_F Primer3: not found at this Tm N.d. 0.06
mm4_exon_Meis2_chr2_116049118_R Primer3: not found at this Tm N.d. 0.06
mm4_exon_Cltb_chr13_54611073_F Primer3: not found at this Tm N.d. 0.06
mm4_exon_Cltb_chr13_54611073_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Tas2r144|Gstk1_chr6_42218614_F TCGTCGGCAGCGTCAGTGACAAAGTGGCATCCGG 60.6 0.06
mm4_intergenic_Tas2r144|Gstk1_chr6_42218614_R GTCTCGTGGGCTCGGCCCAGAGAAACTCCCTGTGT 58.9 0.06
mm4_intergenic_Gm5570|n-R5s162_chr6_60205224_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm5570|n-R5s162_chr6_60205224_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Atg2b_chr12_105642192_F TCGTCGGCAGCGTCGCTACCAACAAACCCAAATAAACA 58.3 0.05
mm4_intron_Atg2b_chr12_105642192_R GTCTCGTGGGCTCGGAGGAGGATTTGAGTGGATGACA 58.8 0.05
mm4_intergenic_CT030684.1|Gm23276_chr14_97299687_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_CT030684.1|Gm23276_chr14_97299687_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Nckap5_chr1_126254831_F TCGTCGGCAGCGTCATAATAGGCCAGCCAGGGTG 59.2 0.05
mm4_intron_Nckap5_chr1_126254831_R GTCTCGTGGGCTCGGCCCTCTCGATTCATCTGGCTC 59.9 0.05
mm4_intron_Pknox2_chr9_36981509_F TCGTCGGCAGCGTCTGCCAGTAAAACCAACAGTTCTG 59.6 0.05
mm4_intron_Pknox2_chr9_36981509_R GTCTCGTGGGCTCGGCACAGTGGTGTAGTGACCCC 59.9 0.05
mm3_intergenic_Macrod2|Gm14080_chr2_141100063_F Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Macrod2|Gm14080_chr2_141100063_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm26651|Diras2_chr13_52069972_F TCGTCGGCAGCGTCGTCTGTCCTCTAAGTGTCAAGCA 59.9 0.05
mm4_intergenic_Gm26651|Diras2_chr13_52069972_R GTCTCGTGGGCTCGGTCAAATCCCACAGCCTTCCC 59.9 0.05
mm4_intergenic_Tmem117|Nell2_chr15_95209426_F TCGTCGGCAGCGTCGGCCCAGAGGATAGCAAAGG 60.1 0.04
mm4_intergenic_Tmem117|Nell2_chr15_95209426_R GTCTCGTGGGCTCGGTGTCAAAACAGGAGCTCCCC 59.8 0.04
mm4_intergenic_Gm23829|Gm5974_chr1_48166819_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm23829|Gm5974_chr1_48166819_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gucy1a3|Map9_chr3_82300388_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gucy1a3|Map9_chr3_82300388_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Pde4c_chr8_70729728_F TCGTCGGCAGCGTCCGAAGTGACTGGCTCTGCTT 60.3 0.04
mm4_intron_Pde4c_chr8_70729728_R GTCTCGTGGGCTCGGGACCTAGACGTGTGCACACA 59.9 0.04
mm4_intron_Dpp8_chr9_65049922_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Dpp8_chr9_65049922_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Peli2|Gm23881_chr14_48361055_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Peli2|Gm23881_chr14_48361055_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Aprt_chr8_122575908_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Aprt_chr8_122575908_R Primer3: not found at this Tm N.d. 0.04
mm4_exon_Tor1aip2/Tor1aip1_chr1_156035584_F Primer3: not found at this Tm N.d. 0.03
mm4_exon_Tor1aip2/Tor1aip1_chr1_156035584_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Ccnh_chr13_85192970_F TCGTCGGCAGCGTCAGTGTGGCTCAATACAGGCC 60.0 0.03
mm4_intron_Ccnh_chr13_85192970_R GTCTCGTGGGCTCGGTCAGTTTCAGCCCAGTCCAG 59.6 0.03
mm4_intergenic_Rreb1|Ssr1_chr13_37965179_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Rreb1|Ssr1_chr13_37965179_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Akap12_chr10_4298393_F TCGTCGGCAGCGTCTGGTGCGAAAGAGATCATGGT 59.7 0.03
mm4_intron_Akap12_chr10_4298393_R GTCTCGTGGGCTCGGCAAGCTTGCATGGACAGGC 59.7 0.03
mm4_intron_Gm21002_chr8_21392056_F TCGTCGGCAGCGTCTCCTTTGGAGACCCAGAAGGA 60.1 0.03
mm4_intron_Gm21002_chr8_21392056_R GTCTCGTGGGCTCGGGCCTTGGGAGCCACATTACT 60.0 0.03
mm4_intron_Gm15308_chr8_21529275_F TCGTCGGCAGCGTCTCCTTTGGAGACCCAGAAGGA 60.1 0.03
mm4_intron_Gm15308_chr8_21529275_R GTCTCGTGGGCTCGGGCCTTGGGAGCCACATTACT 60.0 0.03
mm4_intron_Defa2_chr8_21378760_F TCGTCGGCAGCGTCTCCTTTGGAGACCCAGAAGGA 60.1 0.03
mm4_intron_Defa2_chr8_21378760_R GTCTCGTGGGCTCGGGCCTTGGGAGCCACATTACT 60.0 0.03
mm4_intron_Defa20_chr8_21509501_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Defa20_chr8_21509501_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_5S_rRNA|Cops8_chr1_90334747_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_5S_rRNA|Cops8_chr1_90334747_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Rbm33|Shh/9530036O11Rik_chr5_28456605_F TCGTCGGCAGCGTCAGAGTTGGAAATGGGCAGGG 59.9 0.02
mm4_intergenic_Rbm33|Shh/9530036O11Rik_chr5_28456605_R GTCTCGTGGGCTCGGGCCCTCCTCATTGGAAGACC 60.1 0.02
mm4_intergenic_D230002A01Rik|Gm24254_chr8_91903848_F TCGTCGGCAGCGTCGTTGCTGCCAAAGTCAGTGG 59.9 0.02
mm4_intergenic_D230002A01Rik|Gm24254_chr8_91903848_R GTCTCGTGGGCTCGGGACCTGTGGTCCATCCTCAC 59.7 0.02
mm4_intergenic_Kif2b|Gm11498_chr11_91632239_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Kif2b|Gm11498_chr11_91632239_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Arhgap8_chr15_84737324_F TCGTCGGCAGCGTCCGTGTGCCCTTCCTTCTGAA 60.2 0.02
mm4_intron_Arhgap8_chr15_84737324_R GTCTCGTGGGCTCGGTAAAGGCCAGAGCTACGCAG 59.8 0.02
mm4_intergenic_6720401G13Rik|2610018G03Rik_chrX_50759780_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_6720401G13Rik|2610018G03Rik_chrX_50759780_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Prdm11|Gm13802_chr2_93065246_F TCGTCGGCAGCGTCAGCAGCTATTAGGAACCGGC 59.8 0.01
mm4_intergenic_Prdm11|Gm13802_chr2_93065246_R GTCTCGTGGGCTCGGCTTACCAGCTGGCTGTCCAA 59.9 0.01
mm4_intron_Csmd2_chr4_128299767_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Csmd2_chr4_128299767_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Muc1_chr3_89231777_F TCGTCGGCAGCGTCGAAATCCACCTCCCTTGGGG 60.0 0.01
mm4_exon_Muc1_chr3_89231777_R GTCTCGTGGGCTCGGATACAGACCAGCACCAGCAG 59.7 0.01
mm3_intron_Atf2_chr2_73819577_F Primer3: not found at this Tm N.d. 0.01
mm3_intron_Atf2_chr2_73819577_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Serp2|Lacc1_chr14_76728742_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Serp2|Lacc1_chr14_76728742_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Gm16998_chr10_54075903_F TCGTCGGCAGCGTCGGAGTTTACGGGAAGCGCT 60.0 0.00
mm4_exon_Gm16998_chr10_54075903_R GTCTCGTGGGCTCGGCGAGTCTTAACACGGCTCCC 60.4 0.00
mm3_intergenic_Gm13750|Gm25748_chr1_64174877_F Primer3: not found at this Tm N.d. 0.00
mm3_intergenic_Gm13750|Gm25748_chr1_64174877_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25985|Gm6404_chr13_115461968_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25985|Gm6404_chr13_115461968_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Tmem232_chr17_65448523_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Tmem232_chr17_65448523_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Sufu_chr19_46447214_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Sufu_chr19_46447214_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Ntn1_chr11_68387121_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Ntn1_chr11_68387121_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Mtmr3_chr11_4484476_F TCGTCGGCAGCGTCCCACTGGCTTTCCCCAAAGA 60.1 0.00
mm4_intron_Mtmr3_chr11_4484476_R GTCTCGTGGGCTCGGGCAACTGATGGAAGAAGCTGC 60.1 0.00
mm4_intron_Gli2_chr1_119005029_F TCGTCGGCAGCGTCTGATTCCCACAGGCAACACA 59.8 0.00
mm4_intron_Gli2_chr1_119005029_R GTCTCGTGGGCTCGGCGGGCATTTCCAGAGCTGTA 60.1 0.00
mm4_intergenic_Zfy2|Gm22119_chrY_2424257_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Zfy2|Gm22119_chrY_2424257_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Ryr2|Mtr_chr13_12164622_F TCGTCGGCAGCGTCACACTGCAGCAGTAACTCACA 59.8 0.00
mm4_intergenic_Ryr2|Mtr_chr13_12164622_R GTCTCGTGGGCTCGGGGTGGTTCGGACTGTCCTTT 59.8 0.00
mm4_intergenic_Mlycd|Osgin1_chr8_119424427_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Mlycd|Osgin1_chr8_119424427_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25693|Gm12129_chr11_38059073_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25693|Gm12129_chr11_38059073_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Acaa2|Gm24559_chr18_74901973_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Acaa2|Gm24559_chr18_74901973_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Taf11_chr17_27908880_F TCGTCGGCAGCGTCACTGCAGAAGTAAAACTCTCAGGT 59.8 0.00
mm4_exon_Taf11_chr17_27908880_R GTCTCGTGGGCTCGGGCGCGGAGATCTAGAACCAG 60.3 0.00
mm4_exon_Map3k3_chr11_106123795_F TCGTCGGCAGCGTCAGCTCACACTTTTGCCTCCT 59.5 0.00
mm4_exon_Map3k3_chr11_106123795_R GTCTCGTGGGCTCGGGCAGACTGGGAAGGCTTGAT 60.0 0.00
mm3_intergenic_Padi1|4930515B02Rik_chr4_140871160_F TCGTCGGCAGCGTCCTCCAATGTCCTGGGAGCTC 59.8 0.00
mm3_intergenic_Padi1|4930515B02Rik_chr4_140871160_R GTCTCGTGGGCTCGGTGCCTCTGTGTTTCCGAGAC 59.9 0.00
mm2_intron_Ppa1_chr10_61653065_F TCGTCGGCAGCGTCCCATAAGACTCCGCCGTCTC 59.9 0.00
mm2_intron_Ppa1_chr10_61653065_R GTCTCGTGGGCTCGGGTCAGCCAAGGGTTACCACA 59.8 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313820 AGGGAGCCTGGGATTAGGAGCTTACTTGGGGGTTTTCCCCCTCCCCCCCTCTGGAGAGTCCGGGGATGGAGAGCCGAAAG
GACATG
mm3_intron_Sufu_chr19_46477777 AGTTGCTGTGCTAGGAGCTGCTTAATCAAAGGCCTGTTATGGGCTCATTAGTGTGGTCCACCACGGGCCACCCCTCAGGA
GAGTCCTGG
GAAGGCTGGCTTAGGTCCCGCCCTTCAGGCACGCACCCTTGATCACCAAG
mm4_intergenic_Gm21614|Gm23249_chr12_93537839 ACACAGCTCTTGTTTCTGACATGTTTGCATAGATGCTGTCATCTCCTCTTCTGGAAAGTCCCGGTGAGGTATTCTGTTAT
TAAAATTAGTACCAAGCACTTTTATATAAGTGGAAAGTGCTGTTAAATTCAGT
mm4_intron_Acpp_chr9_104310397 TGGTCAGCCAGCCTTGTTAGCTTCCACATAGCACATTTGCAGACTCGATCTTCATTTCCTTCTATCCAAACCCTGGACTC
TTCTGAGGGGTTGA
GTGAGGTAGACTAACAACTGCCACTTCCAGTTTGTCT
mm4_intergenic_Igfbp5|Tnp1_chr1_72877283 AGTCCATTCTCTCTGCCCTCTATTCGAAAAGCCCTGGACTCTTCAGAAGATGGGAGGGGGGGGGACTTAATATCAACAGA
GTTTCACTGAGATGACTAACTTATTCTGGACACACTGAAATCTGGTCGCACTCTCAGAGA
mm4_intergenic_Gm24291|Mon1b_chr8_113551666 GCGTTGTTCTGAACCGGAAGAAGTGAAGACAGCTTCCTCCTACCCCCATCTCAGAGCAGAGCAACTTCCTCTTGACTTTC
CCTCCCCTCCCCTGGAGAATCAGGGAGTAGCAGGTAACACTCCAAGGGAGGAAGGAGAGG
mm4_intergenic_Zfp358|Mcoln1_chr8_3500010 TGCAAAGCCTTGGGAAGTCACCAGGAGGTTCTAGAAGGTGGAAATTCTTTGGAGGTCCTGGACTCTCCGTAGGAGGAGAC
TGGCTGTAAAAGATAGGACTGAGGCTGTTGCAGTAGCGCAGAAAGTCT
mm3_exon_Tmem233_chr5_116040632 TCCCGACTAGTGGGTGTTCATTTGCCAGGGACCCGCCTCTGGAGAGTCCTGGAGGAAAAGATCTTACAGTTTTAAAATCA
AGGATCCATTCCTCAGAAACTTCTTTGGGAGTGTGTGCAGATGAG
mm4_intergenic_Gm24696|Myc_chr15_61984264 TTCTAGGGTCTCTGCAGGCTCCCCAGATCTGGGTTGGCAATTCACTCCTCCCCCTTTCTGGGAAGTCCGGGTTTTCCCCA
ACCCCCCAATTCATGGCATATTCTCGCGTCTAGCGCCTTGATTTTCCCCACCCCA
mm3_intergenic_Ajap1|Gm25880_chr4_153683070 AGAAATGCTGGGCACACAGAGGTCCAGAACTTTCCAGAGGGGGGCATGATTTCCACAAGGGCACATTCTCTCTAAGTGGC
TATTTGTCATTCAAAGAGAAGACATGTGTTGCTCATGCAAGCAGATGGAC
mm4_intergenic_Dlk1|Mico1_chr12_109489422 TGTCCTCTCAAAGTCTTGGCTATCATCTTACATGAGATTGGAGAGGTGAATACTCTGTAGTCTTCATGTTGGGGGGATCA
ACTACCCTGTACTCTTCAGAAGGGGTGAGACGGATGATCAGACAGTGGAAATCGGGA
mm3_exon_Nynrin/Gm26751_chr14_55871429 TGCAACTGGATCTTCAGCGTTCTCTGGGAGCTCCTGCCCCTCTGGAGAGTCCGGGGCTTCCTGTCATCTGACGGGGCTTC
ACTACCTCATC
mm4_intron_Stra6_chr9_58129211 TGGGGAAACTCAGCCATCAAAGCCCTGGCTTCTCCAGTGGGGGTGATTCTGAGGGCCAGGGGGATCAAGTTTCGGGCAAA
CGGTT
mm4_intron_Theg_chr10_79581565 CCGTCAGCTGTTACAGGGAGCAACTTACAGAAGGAGAGAATGTTCCCAATGGGGCCTCATCCCCGGGTCCAGGAACCTGG
ACTCTCCAGAGGGTCTGT
CCCAGTGACCATGGCAGCTGTA
mm4_intergenic_Gm5091|Dll1_chr17_15333718 GCAAAGCCAGTCTTTTGTGGAGAACCAAAGGCCCTGGTCTCTCCAGAGGGAAAGAAATGTTAATTGAAAAATTCCTTACA
TAGGTGAAGGCTCTGAAGTCTGCTCAACATAAAAGGTATGAAATCTCCATCGGGCTGT
mm4_intergenic_Gm24120|Nr2f2_chr7_70350082 TTTGGAACTGGACAGGGACGATGATCTCCCCCATCTAACTAGAGATGATGGGTCCCCATCTCTGAAGAGGCCTGGGAAGA
CTTTCTGGGAGGCCAATCTGTGCTCCTGCCAGTC
mm4_intergenic_Il23a|Gm23241_chr10_128298200 CAGGTGACTCAGAGCAGGTGGGATTCCCCTCCCTACATCATCTCCCTCTTTGGAGAGCCCAGGTATGCCGCTTCAGTTCC
AGGGGAAATGAGTGGTGAGGTTGTTGTGGCTAGAGGC
mm4_intergenic_4930552P12Rik|Tcf7l2_chr19_55733085 GGAGAGGAACAGGGTGAGGACCTAGGCTTCCCAGGTGCCAGGACTTAGGTAGTCCTGGCATTCCAGGCCCTCTGGAGAGT
CCAGG
AAGGGAAAGGGAACTCACCCTAGGAAAAGCGACAGC
mm4_intron_Tnfrsf21_chr17_43040237 ACGGCCATGGTGAGTTTCTTCCGCTTCCTCTCTGCGTGCAGAGCGCGCGTCCTCATTCCTGGTCTCTCAAGGGGTGGGGA
ATCTGTAGCTTGTGGAGTCCCTTTTCCTCACC
mm4_intergenic_D3Bwg0562e|4833424O15Rik_chr3_117523850 CTTCCTCAGAACCACCGGACTTTGCATTCACATGTCTTCACTGCTAAGTCCAGGGCCAGACATGGCTAAAGCCTGGGCTC
TCCAGAGGAAGGGG
GAAAGAGCATGAGGAGTCTCCCC
mm4_intergenic_Dytn|Gm12748_chr1_63666867 TCATGCAGTCTGGCCTCAAATTCCTGAGCTCAAATAATCGTCCTACCTCCCCATCTCGAGAGTCCCGGGAATAGACACAT
GCACACAAAACTGCA
mm4_intron_Ryr2_chr13_11742770 GGTCCTGACAACCTACCTGCTTGCCTCCAATAGGAGCCTATGACAACATAGTTGCCATCTCTGCCCCAGCTTTAGCTTTT
CCTGCCCTTTGGAGAGCCCTGG
ACTGCTTTCTACAGGAGTTCTCC
mm3_intron_Plekha7_chr7_116288628 GGGATGACTACCTGGGGACTCAGTCCACCCCTCTGCTGAGTCCTGGGAGGAGGGACGGGCAGGAGGTAGTCCTCAGTAGT
GAGAGGAAGTTGCTGGC
mm4_exon_Fbxl2_chr9_113984093 CACTAGGGCTTAACACCCGGTCCTGGATTCTCCTGAGCGTGGGACTCAGAGGGAGCAAAAAGAAAAGTAAAACCTCATTC
AAGCACACTGGGGCTCACACTGTCAACTCCT
mm4_intergenic_C430002E04Rik|Phf17_chr3_41498945 GCATGGCCTAGCACAGATCTCTCAGGAATAAAGTCACGTTTAGGCAGGGAGTAATTGAAATACAGATCACTCAGAAAAGA
TATTCCCTTCCCCTGGAGAGGCCTGGTTTGTTCAGCTGGCGATACACTGG
mm4_intron_Dgat2_chr7_99176682 AGGACCAAGCTCAGAGGGATGTCCCCGGACTGTCCTGGGGAGGGGATACAACAGCTCAGATGAGGTATCAGCCTAACATA
AGGAAGTCATGGGCCCTG
mm4_intergenic_Gm26651|Diras2_chr13_52457254 CCGTAAATGGGTACAAATTCTTCTTAGTTTAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACCCTGTCT
TGTTTGGGACCCCCCCTTTGCAGAGCCCAGGCAGTATCTACCTTCTGAGTAAACTAGGCA
mm4_intergenic_Sppl2c|Mapt_chr11_104196716 CCCACCCACAAGACTTGACTAGAGGAGGGTTTCTAAATCATGGTGTTTGCAGAGGTCTTCCAGAACTCTCCAGAGCTGTG
GA
CAGAGAGAAAGCAGCTTGCCCAA
mm4_intergenic_Gm16477|Gpr26_chr7_131728218 TCATGAACCTGGAAGGCTGGGCACTCAGAGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTGTGACTCAG
CGGCTCCCCAGGGCTCTCCTGAAGGCGGGAGAGGCTGGCTTAGTGGGAGCTAA
mm4_intron_Hps4_chr5_112364958 GCATCCGTACTCACTGCCAAGGCAGATCGGACCTCGGCCAGTGCTCTCCAGAGGTGGGGCTAGAACTGGTCAAGCCCTGG
GTTCCCTTCCTCTCTGCTCTGCCATCCTG
mm4_intron_Gsk3b_chr16_38182225 TGCTTTGTTAAACTGGCTGCCTAAAAGGATTGTTGTCAGTACTTGTAACGAGGCCTGAACTCTCCAGAGCAGAGGAGTTA
TCTGTGTCGGTCTAAACACAGCACACACAGCAC
mm4_intron_Rnls_chr19_33369969 GCAGTGGGCATCTCTTCTGTTCCTGGTGACGCCAGGACTTTGCAGAGGGTGGCAGAGAAAAAAGCTAAATTTGGGTTGTT
GTTCATTTAGAGATAGAAAAGTAAGCAGAAATGTATCCAAATTTGCTGTTTTGCA
mm4_intergenic_Mir29b-1/Gm27019|Gm13834_chr6_31068371 AGCGAGGCTCACAATGTACCCAACTCCAGGGAGAAGACAGCACCTCGCTCTGAAGGGCTGGCCCTGGTCTCTGCAGAGGG
AGGGG
TTTCCCTCTCCTTTAAGCCCTGCTCCTCAGGAGTATCAGCCTGCG
mm4_intergenic_Dctd|Tenm3_chr8_48167551 AATGTGTGCCAGGACCTGACTTCCAAGTTCACCATCTTTCCATGGCTCTCCTGAAGGGGGGAAAATGACTTCCCCATCTC
CCTCCCTCCAGAA
mm4_intergenic_Skor1|Map2k5_chr9_63158769 CCCGCCTCTCTTTTCCATGTGTGGGCCATTGCTCTCCTTGTCCCCTGGGTTCCCCAGAGTGGGGGAAAAGACTGAAAGAA
TGAATTCCTAGGAGAGCCCAGCA
mm4_intron_Rbms3_chr9_116711545 TCCATCCTGGGAGATTCCTGTTATGACTGCCATTACCCAGTTCTCTCCAGATTGGGGGAAGTTGTAGAGGCAGCTCTAGG
GTTGACCAG
mm4_intergenic_Gm27034|Rpl36a-ps1_chr14_98845936 TGATGGAAGGCTGAGTGCTGCCTTCCCATTTGGGTCCTGGACTCTCCACCAGGAGGGAGAACTGTCACTTCCACCATCTC
ACCTTTTGCGATGCCT
mm3_intergenic_Gm16079|Gm13049_chr4_150780826 CCCTGGGTTCTTTCGACCTCACATGGCTTTGCTGCCTCTCTCTTCCTGGACTTTACAGAGCGGGGGATGAAAAGGTCTGC
AGCAGGT
mm3_intron_Fads2_chr19_10100899 GGCCATCTTCCACTTGGGGAGCGCCGAACACGAAGGCTGGAGCGCCCCCTTTGGAGAGTCCCAGGCTCCGGCTGGAACAG
CGCGCAGTATGCGCTGTGAACATGCA
mm4_intergenic_Gm22492|Pdcd6ip_chr9_113455155 TCTGCTCTCAAAACAGCCCAAAGCATCCCGGCCGAAAGATGGAGCAGCCCGTTCCACTGCTCTGGAGAGTCTAGGCCAAC
AAGTGAGATGACAACAGCAGGAAAGAGACCGTGGAGCAGAGAT
mm4_exon_Sncaip_chr18_52768446 TCTGTCCGTCCATCCCTCCCGCCGTCCTCCCGCGCTCCGGTGAGTCCAGGTCCCAACCGCGGGCGCCGCAGGGAGGAGAG
GGACGAGGGCAGCCCGTGCAACTTTGCACCGGGCGACACCCTGAGGGTTTTCGGGACC
mm4_intergenic_Six2|Srbd1_chr17_85696625 GTCCCTGTTTGCCTCTGGAAGGCTGAAGGCAGCANNNNNNNNNNNNNNNNNNNNNNTGGTGAGGCCTGGCTCTGCTCATG
GCCCCACTGAGTTCTT
mm4_exon_Ankrd33_chr15_101119627 GCACGGTATGAGCTGACACTCAGGCCTCAGGGGCAGCAAAGTCTGGCTCCTCCAGTCTGGAGATTCCAGGAGAGGAAGAA
GAAGGAGGAAGAAACAGAACCACGAGGAGGTG
mm4_intergenic_Gm5054|Ifitm10_chr7_142320863 GCTGCTGTCCCCTCTATTCCTGGCTTGGTTTACCAAGCCTCTGGATGTGAGTGGGATGCCAGGACTCTGGGGAGGGAGGG
A
ACCGTGAGTTTCTTGGGGTTACCTCCTGTTGGGACCATGACCTTACCCT
mm4_intron_Ccdc85c_chr12_108245829 GTGATTCGACCTCCCTGAGCCGCAGAGCTGGGACTCTCCAGAGGGAAGCCCTGTCAGTCAGCGTGGGGGGCGGGAGAATG
TTGGTCCTCGTTTAGACACAGGCTGTGGGACAGACGGTGTCCTTCATTGCGAT
mm4_intron_Gm3054_chr5_137165146 CGTAGCAGGGCAAGGTTGTATTCCCCCATGCTTCTCGAGAGGGGGGGATGGTATTAGACCCAGAGGAAGTATCAAGAGCT
TCAGGATGGAGGAGG
mm3_intergenic_3110083C13Rik|Fgf9_chr14_58036449 TGGCGTTGAAAGGGTAGGACCTCTAACGAGCTGTTTCTGTCCCTAATGGCTGGGAGCCTGGAAATTAGCACAGTGCCTGC
TGTTCTACATGAGTGACCCGGCCTCTGGAGAGTCCTAGTCCCTCCAGGAAGGTTCTGT
mm4_intergenic_Gm10858|Gtf3a_chr5_146914068 TTGTCTACACAGTGGCAGGCTGCATGGTGCTCACCGCTCCTCTCCCTTGGGCACCAAGATGCCTCACCCACTCTGAAGAG
TGCTGG
AAACCACTGACCTGGTCTTGTGGGTTCTGGCAAGGGAAACCT
mm4_intron_Aldh1a2_chr9_71236734 TGTTGCCAAACAGCTGTGACTAGTTCATAAACACTTTGGCAATTGATGAATTTCTCTTGCTCAGCTTGGATACCCAGACT
CTGCAGAGGAAGGGA
GTGTGAAAAGGCACTTCACTCACTCAGA
mm4_intron_Tsku_chr7_98355068 ACAGCCCAGATGTTGCTCTCCTCTGGGCCCTGGCCCTGGACTCTGCACAGGGAGGCAAAGTCTATCCTGCCATCCTCACA
CAAGCCAACAAACCCCTG
mm4_intergenic_Gm20424|Lbx1_chr19_45201003 GGCATTTCAGACTGGGCTCTATTGCTCCTGAAATGCAACCCCCAAGGCCAACCTCTCCCTCTGGAGGGTCCAGGTGAAGG
GGTTTGCAGGAGGAA
mm4_intergenic_Efnb1|Gm14812_chrX_99187257 TGGCCCTCTCTGAGACATGAAGGGCCTTGGCAGGATGGATGGTTCCCACCCTGAGCAGAGTCCAGGAAGAAAGCAACTGG
TCAGCCCTGTCACTTT
mm4_intergenic_Tas2r144|Gstk1_chr6_42218614 AGTGACAAAGTGGCATCCGGCTTCCAGAAGTCTCCAGAGGGGAAGAAGAAATGATGAGAGAGCATGTAAAGAGAGAGTCT
GTAAAGACTAGGAACACAGGGAGTTTCTCTGGG
mm4_intron_Atg2b_chr12_105642192 GCTACCAACAAACCCAAATAAACATAAATATAGCTCAGGAGCAGACAGAGAACCAGGACCCTGCAGAGGGGAGGGGTTCC
TTAAAACCCTGCTGGGCAGCCAGGTATCCCTGACCAAGTGTCATCCACTCAAATCCTCCT
mm4_intron_Nckap5_chr1_126254831 ATAATAGGCCAGCCAGGGTGGCAGGAAGATGCAGCATGGTTTTTCTGTAAGCCTGGACTATCCAGAGAGTAGGAGCCACA
GAGCCAGATGAATCGAGAGGG
mm4_intron_Pknox2_chr9_36981509 TGCCAGTAAAACCAACAGTTCTGAATATTGTGACCACATAAGGAAATGTTGTAAAAGGAGCCATGACTCTACCGAAGGGG
GGA
ATCCAGAGGGACAGGAATGGGGTCACTACACCACTGTG
mm4_intergenic_Gm26651|Diras2_chr13_52069972 GTCTGTCCTCTAAGTGTCAAGCACATGCTATGACTAACTAGCAAACCCTGTGTCTCCAGAGGGAGGGAATCCTAGCATCC
TGAGGCTTCAGGGGAAGGCTGTGGGATTTGA
mm4_intergenic_Tmem117|Nell2_chr15_95209426 GGCCCAGAGGATAGCAAAGGAATGTCAGCCGGCCGCTGTCCCTCCCTCCCTCTGAAGGGTACCGGAACTTAGGGGGAGCT
CCTGTTTTGACA
mm4_intron_Pde4c_chr8_70729728 CGAAGTGACTGGCTCTGCTTCTGTCCCCAGCTCTTGAGATTCCTGGCCTGTGATTTTGTGTGCACACGTCTAGGTC
mm4_intron_Ccnh_chr13_85192970 AGTGTGGCTCAATACAGGCCATCTCATCAGAAAATGGCTCTCTTGCCATGACTAGAATCCTAGACTCCTCCCCTCTGGTG
ACTCTTGG
AGTGCCTAGCCTGGTAATCCTCTGCTTCTGGCTGGACTGGGCTGAAACTGA
mm4_intron_Akap12_chr10_4298393 TGGTGCGAAAGAGATCATGGTCCAGGAATCAACAGAGTGGGGGATGTTATCAAATTCATGATCCATCCTAAAACTTTGCC
TGTCCATGCAAGCTTG
mm4_intron_Gm21002_chr8_21392056 TCCTTTGGAGACCCAGAAGGATCTGCTCTTCATGAAAAATGTGAGTAATGGTACCCAGTGTGATGGATGCCTGCATTCTC
CCGAGGGAGGGA
TGAAGATGAGTCCTAGAATCCTGGTAGAGTAATGTGGCTCCCAAGGC
mm4_intron_Gm15308_chr8_21529275 TCCTTTGGAGACCCAGAAGGATCTGCTCTTCATGAAAAATGTGAGTAATGGTACCCAGTGTGATGGATGCCTGCATTCTC
CCGAGGGAGGGA
TGAAGATGAGTCCTAGAATCCTGGTAGAGTAATGTGGCTCCCAAGGC
mm4_intron_Defa2_chr8_21378760 TCCTTTGGAGACCCAGAAGGATCTGCTCTTCATGAAAAATGTGAGTAATGGTACCCAGTGTGATGGATGCCTGCATTCTC
CCGAGGGAGGGA
TGAAGATGAGTCCTAGAATCCTGGTAGAGTAATGTGGCTCCCAAGGC
mm4_intergenic_Rbm33|Shh/9530036O11Rik_chr5_28456605 AGAGTTGGAAATGGGCAGGGGAAGATTGTCCCCCTCAATTCCCCAACTCTGGAGAGTGTGGGCGGAGCTTTGCATAGCAA
GTACAGGGCTGGGGTCTTCCAATGAGGAGGGC
mm4_intergenic_D230002A01Rik|Gm24254_chr8_91903848 GTTGCTGCCAAAGTCAGTGGATGAGCCTGGATTGTCCGGGACTATGCAGAGGGTGGTATCCATGTGCTCTGGCTCAGTTG
CCTTTCTCATGTGGAGATGGACAATGGGGGTGAGGATGGACCACAGGTC
mm4_intron_Arhgap8_chr15_84737324 CGTGTGCCCTTCCTTCTGAAAATCAAGATAGAGGTAGCCTGAGGTGCAGTGTCCCCCCCTCAGGAGGGGACAGGTTCAGC
CATGTCACAGCAGCATCTGCGTAGCTCTGGCCTTTA
mm4_intergenic_Prdm11|Gm13802_chr2_93065246 AGCAGCTATTAGGAACCGGCAACTACCTGGAGCCTCCACAGGAGGGGAACTTGCACACAGTCCACGACTTCTATTAAGAT
CATAGAGGACTGACACTAAACAGAGTTGGACAGCCAGCTGGTAAG
mm4_exon_Muc1_chr3_89231777 GAAATCCACCTCCCTTGGGGACTTCCCTGACCACCGCTTTCCCTTCTAGTGAATGAGATGCAGTTCCCTCCCTCTGCCCA
GTCCCGG
CCGGGGGTACCAGGCTGGGGCATTGCCCTGCTGGTGCTGGTCTGTAT
mm4_exon_Gm16998_chr10_54075903 GGAGTTTACGGGAAGCGCTGCCTCTCCGGGACCCGGCAGAGGCGGGGAGGTCGCGGCTCCCCTGGGCTCCCGAGGACTGG
AACTGCGCCCGGGAGCCGTGTTAAGACTCG
mm4_intron_Mtmr3_chr11_4484476 CCACTGGCTTTCCCCAAAGACGAGCCCTGCAGGAGCATCCCTTCTCATCAGGAGTCTCAGAGCCCAGCTGGCCTGGACAG
TCCAGAGGGGAGCA
CACGGCTGCAGCGGACACACCCCTGGCAGCTTCTTCCATCAGTTGC
mm4_intron_Gli2_chr1_119005029 TGATTCCCACAGGCAACACAAAAGCTTCTTCAAAGAAGGGTCTGAGAGCCCCAGGACACTCCAGGGGAGGAGAGTCAACA
CCCCACTTTCTTTTACAGCTCTGGAAATGCCCG
mm4_intergenic_Ryr2|Mtr_chr13_12164622 ACACTGCAGCAGTAACTCACATCCAGGCCACTCCAGAGAGGAGGAACATGTAGTGCACCTTAGTGGACATAAAAGGACAG
TCCGAACCACC
mm4_exon_Taf11_chr17_27908880 ACTGCAGAAGTAAAACTCTCAGGTCTTCCCTCCAGGAGGCACCAGAGGGAGGGATTTTTCAAGTCTGGTTCTAGATCTCC
GCGC
mm4_exon_Map3k3_chr11_106123795 AGCTCACACTTTTGCCTCCTCTCTTATAGACTAGTTCCTCTCCCCACTCTGGAGTGTCCAGGCAGGTTCGGATCAAGCCT
TCCCAGTCTGC
mm3_intergenic_Padi1|4930515B02Rik_chr4_140871160 CTCCAATGTCCTGGGAGCTCCTTGCGGGCTGAATCCCAACCTTGTAATTAAGTGTAGGGCTGGGCACTGCTTCCTGGACG
CTCCACAGGGGGGGC
AGTCTCGGAAACACAGAGGCA
mm2_intron_Ppa1_chr10_61653065 CCATAAGACTCCGCCGTCTCAGAGAACCCTGAGAAATGATGTTTTGTAGGACAGCCAAGGGTCATCTCCATCCCCCCTTC
TGGAGTGTCCCGA
GGCAGCTGTGGTAACCCTTGGCTGAC

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_mN1FkRligAThyqKv3eg3.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.