CRISPOR

DNA break repair outcome predictions

Guide: TGGGGGTCAGCTTGGCACTC TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GACATGGTTATGTTTCTGGATGGGGGTCAGCTTGGCACTCTGGTTGGTAAGAGGGTCTCTAATTTGTCCGAAGCCGTGAG`	(Wild-type sequence)
335	`TGTTTCTGGATGGGGGTCAGCTTGG--------TTGGTAAGAGGGTCTCTAATTTGTCCG`	`8 bp deleted → frameshift`
329	`TGTTTCTGGATGGGGGTCAGCTTGG------------TAAGAGGGTCTCTAATTTGTCCG`	`12 bp deleted → no frameshift`
213	`TGTTTCTGGATGGGGGT-----------------TGGTAAGAGGGTCTCTAATTTGTCCG`	`17 bp deleted → frameshift`
211	`TGTTTCTGGATGGGGGTC-----------------------------TCTAATTTGTCCG`	`29 bp deleted → frameshift`
193	`TGTTTCTGGATGGGGGTCAGCTTGGCACTC-------------------TAATTTGTCCG`	`19 bp deleted → frameshift`
184	`TGTTTCTGGATGG--------------------TTGGTAAGAGGGTCTCTAATTTGTCCG`	`20 bp deleted → frameshift`
175	`TGTTTCTGGATGGGGGT---------------------AAGAGGGTCTCTAATTTGTCCG`	`21 bp deleted → no frameshift`
162	`TGTTTCTGGATGGGG------------------TTGGTAAGAGGGTCTCTAATTTGTCCG`	`18 bp deleted → no frameshift`
154	`TGTTTCTGGATGGGGGTCAGCTTGG-------------------GTCTCTAATTTGTCCG`	`19 bp deleted → frameshift`
154	`TGTTTCTGGATGGG-------------------TTGGTAAGAGGGTCTCTAATTTGTCCG`	`19 bp deleted → frameshift`
150	`TGTTTCTGGATGG------------------------TAAGAGGGTCTCTAATTTGTCCG`	`24 bp deleted → no frameshift`
150	`TGTTTCTGG------------------------TTGGTAAGAGGGTCTCTAATTTGTCCG`	`24 bp deleted → no frameshift`
147	`TGTTTCTGGATGGGGGTCAGCTTGG--------------------TCTCTAATTTGTCCG`	`20 bp deleted → frameshift`
133	`TGTTTCTGGATGGGG------------------------------TCTCTAATTTGTCCG`	`30 bp deleted → no frameshift`
133	`TGTTTCTGGATGGGG----------------------TAAGAGGGTCTCTAATTTGTCCG`	`22 bp deleted → frameshift`
128	`TGTTTCTGGATGGGGGTCAGCTTGGCACTC-----------------TCTAATTTGTCCG`	`17 bp deleted → frameshift`
127	`TGTTTCTGGATGGG-------------------------------TCTCTAATTTGTCCG`	`31 bp deleted → frameshift`
126	`TGTTTCTGGATGGG-----------------------TAAGAGGGTCTCTAATTTGTCCG`	`23 bp deleted → frameshift`
123	`TGTTTCTGG----------------------------TAAGAGGGTCTCTAATTTGTCCG`	`28 bp deleted → frameshift`
105	`TGTTTCTGGATGGGGGTCAGCTTGGCACT---------------------AATTTGTCCG`	`21 bp deleted → no frameshift`
105	`TGTTTCTGGATGGGGGTCAG---------------------AGGGTCTCTAATTTGTCCG`	`21 bp deleted → no frameshift`
98	`TGTTTCTGGATGGGGG----------------------------GTCTCTAATTTGTCCG`	`28 bp deleted → frameshift`
95	`TGTTTCTGGATGGGGGTCAG-----------------------GGTCTCTAATTTGTCCG`	`23 bp deleted → frameshift`
84	`TGTT-------------------------------GGTAAGAGGGTCTCTAATTTGTCCG`	`31 bp deleted → frameshift`
81	`TGTTTCTGGATGGGGGTCAGCT--------------------------CTAATTTGTCCG`	`26 bp deleted → frameshift`
80	`TGTTTCTGGATGGGGGTCAGCTTG--------------------------------TCCG`	`32 bp deleted → frameshift`
80	`TGTTTCTGGATGG--------------------------------TCTCTAATTTGTCCG`	`32 bp deleted → frameshift`
74	`TGTTTCTGGATGGGGGTCAGCTTGGCACTC----------------------------CG`	`28 bp deleted → frameshift`
74	`TGTTTCTGGATGGGGGTCAGCT----------------------------AATTTGTCCG`	`28 bp deleted → frameshift`
69	`TGTTTCTGG-----------------------------------GTCTCTAATTTGTCCG`	`35 bp deleted → frameshift`
67	`TGTTTCTGGATGGGGGTC----------------------------------------CG`	`40 bp deleted → frameshift`
66	`TG------------------------------GTTGGTAAGAGGGTCTCTAATTTGTCCG`	`30 bp deleted → no frameshift`
66	`TGTTTCTGG------------------------------------TCTCTAATTTGTCCG`	`36 bp deleted → no frameshift`
63	`TGTTTCTGGATGGGGGTC-------------------------------TAATTTGTCCG`	`31 bp deleted → frameshift`
60	`TGTTTCTGGA--------------------------------GGGTCTCTAATTTGTCCG`	`32 bp deleted → frameshift`
54	`TG----------------------------------GTAAGAGGGTCTCTAATTTGTCCG`	`34 bp deleted → frameshift`
52	`TGT-----------------------------------AAGAGGGTCTCTAATTTGTCCG`	`35 bp deleted → frameshift`
51	`TGTTTCT-----------------------------------------CTAATTTGTCCG`	`41 bp deleted → frameshift`
46	`TGTTTCT-------------------------------------------AATTTGTCCG`	`43 bp deleted → frameshift`
44	`TGTTT------------------------------GGTAAGAGGGTCTCTAATTTGTCCG`	`30 bp deleted → no frameshift`
42	`TGTTTCTGGATGGGGGTCAGCTT-------------------------------TGTCCG`	`31 bp deleted → frameshift`
34	`TGT-------------------------------------------CTCTAATTTGTCCG`	`43 bp deleted → frameshift`
33	`TGTTTCTGGATG--------------------------------------------TCCG`	`44 bp deleted → frameshift`
27	`TGTTTCTG------------------------------------------------TCCG`	`48 bp deleted → no frameshift`
26	`TGT------------------------------------------------------CCG`	`54 bp deleted → no frameshift`
24	`TGTTT--------------------------------------------------GTCCG`	`50 bp deleted → frameshift`
24	`TGTTTCTGGAT------------------------------------------TTGTCCG`	`42 bp deleted → no frameshift`
22	`TGTTTC----------------------------------------------------CG`	`52 bp deleted → frameshift`
17	`TGTTT-------------------------------------------------TGTCCG`	`49 bp deleted → frameshift`
15	`TGTT---------------------------------------------------GTCCG`	`51 bp deleted → no frameshift`

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