CRISPOR

DNA break repair outcome predictions

Guide: TCTGCCACAGTGAAGATGAA AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CAAGTGGATAAATAGAAACGTCTGCCACAGTGAAGATGAAAGGAGACCCGCAGCTAATGGCTCGGCAGTGATGCGCCAGG`	(Wild-type sequence)
242	`AATAGAAACGTCTGCCACAGTGAAGA----------CCCGCAGCTAATGGCTCGGCAGTG`	`10 bp deleted → frameshift`
233	`AATAGAAACGTCTGCCACAGTGAAGATGA-----GACCCGCAGCTAATGGCTCGGCAGTG`	`5 bp deleted → frameshift`
211	`AATAGAAACGTCTGCCACAGTGAAGATGA-------CCCGCAGCTAATGGCTCGGCAGTG`	`7 bp deleted → frameshift`
211	`AATAGAAACGTCTGCCACAGTGAAG-------GAGACCCGCAGCTAATGGCTCGGCAGTG`	`7 bp deleted → frameshift`
201	`AATAGAAACGTCTGCCACAGTGAAGA--------GACCCGCAGCTAATGGCTCGGCAGTG`	`8 bp deleted → frameshift`
173	`AATAGAAACGTCTGCCACAGTGA-----------GACCCGCAGCTAATGGCTCGGCAGTG`	`11 bp deleted → frameshift`
164	`AATAGAAACGTCTGCCACAG------------GAGACCCGCAGCTAATGGCTCGGCAGTG`	`12 bp deleted → no frameshift`
158	`AATAGAAACGTCTGCCACAG-----------------------CTAATGGCTCGGCAGTG`	`23 bp deleted → frameshift`
156	`AATAGAAACGTCTGCCACAGTGA-------------CCCGCAGCTAATGGCTCGGCAGTG`	`13 bp deleted → frameshift`
141	`AATAGAAACGTCTGCCACAG---------------ACCCGCAGCTAATGGCTCGGCAGTG`	`15 bp deleted → no frameshift`
140	`AATAGAAACGTCTGCCACAGTGAAGATG---------------------GCTCGGCAGTG`	`21 bp deleted → no frameshift`
133	`AATAGAAACGTCTGCC----------------------CGCAGCTAATGGCTCGGCAGTG`	`22 bp deleted → frameshift`
126	`AATAGAAACGTCTGCC-----------------------GCAGCTAATGGCTCGGCAGTG`	`23 bp deleted → frameshift`
122	`AATAGAAACGTCTGCCACAGTGAAG------------------CTAATGGCTCGGCAGTG`	`18 bp deleted → no frameshift`
120	`AATAGAAACGTCTGCCACAGTG--------------------------------------`	`38 bp deleted → frameshift`
116	`AATAGAAACGTCTGCCAC-------------------CCGCAGCTAATGGCTCGGCAGTG`	`19 bp deleted → frameshift`
109	`AATAGAAACGTCTGC--------------------------AGCTAATGGCTCGGCAGTG`	`26 bp deleted → frameshift`
94	`AATAGAAACGTCTGC-----------------------------TAATGGCTCGGCAGTG`	`29 bp deleted → frameshift`
89	`AATAGAAACG------------------------------CAGCTAATGGCTCGGCAGTG`	`30 bp deleted → no frameshift`
89	`AATAGA------------------------------CCCGCAGCTAATGGCTCGGCAGTG`	`30 bp deleted → no frameshift`
86	`AATAGAAACGTCTGCCA-------------------------GCTAATGGCTCGGCAGTG`	`25 bp deleted → frameshift`
85	`AATAGAAACGTCTGCCACAGTGAAGATGAA-----------------TGGCTCGGCAGTG`	`17 bp deleted → frameshift`
77	`AATAGAAACGTCTGCCACAGTG---------------------------GCTCGGCAGTG`	`27 bp deleted → no frameshift`
77	`AATAG---------------------------GAGACCCGCAGCTAATGGCTCGGCAGTG`	`27 bp deleted → no frameshift`
74	`AATAGAAAC----------------------------CCGCAGCTAATGGCTCGGCAGTG`	`28 bp deleted → frameshift`
74	`AATAGA----------------------------GACCCGCAGCTAATGGCTCGGCAGTG`	`28 bp deleted → frameshift`
66	`AATAGAAACGTCTGC------------------------------------TCGGCAGTG`	`36 bp deleted → no frameshift`
63	`AATAGAAACGTCTGCCACAGTGAA-----------------------TGGCTCGGCAGTG`	`23 bp deleted → frameshift`
60	`AATAGAAACGTCTGCCACAGTGAAGATG--------------------------------`	`32 bp deleted → frameshift`
60	`AATAGAAACGTCT--------------------------------AATGGCTCGGCAGTG`	`32 bp deleted → frameshift`
57	`AATAGAAACGTCTGCCACAGTGAAG---------------------------------TG`	`33 bp deleted → no frameshift`
52	`AATAGAAACGTCTG-----------------------------------GCTCGGCAGTG`	`35 bp deleted → frameshift`
51	`AATAGAAACGTCTGC-----------------------------------------AGTG`	`41 bp deleted → frameshift`
45	`AATAG--------------------------------------CTAATGGCTCGGCAGTG`	`38 bp deleted → frameshift`
44	`AATAGAAACG--------------------------------------------GCAGTG`	`44 bp deleted → frameshift`
42	`AATAGAAACGTCT---------------------------------------CGGCAGTG`	`39 bp deleted → no frameshift`
40	`AATAGAAACGTCTGCCA----------------------------------------GTG`	`40 bp deleted → frameshift`
38	`AATAGAAACGTC-----------------------------------------GGCAGTG`	`41 bp deleted → frameshift`
31	`AAT---------------------------------------------GGCTCGGCAGTG`	`45 bp deleted → no frameshift`
30	`AATAGAAACGTCTG----------------------------------------------`	`46 bp deleted → frameshift`
28	`AATAGAAA---------------------------------------TGGCTCGGCAGTG`	`39 bp deleted → no frameshift`
27	`AATAGAAACGT------------------------------------------------G`	`48 bp deleted → no frameshift`
27	`AATAGAA----------------------------------------TGGCTCGGCAGTG`	`40 bp deleted → frameshift`
24	`AATA------------------------------------------ATGGCTCGGCAGTG`	`42 bp deleted → no frameshift`
21	`AATAG-----------------------------------------------------TG`	`53 bp deleted → frameshift`

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