CRISPOR

DNA break repair outcome predictions

Guide: GACTCTCCAGAGGGGGGGAG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CTTTCGGCTCTCCATCCCCGGACTCTCCAGAGGGGGGGAGGGGGAAAACCCCCAAGTAAGCTCCTAATCCCAGGCTCCCT`	(Wild-type sequence)
666	`TCCATCCCCGGACTCTCCAGAGGGGGGGA------AAACCCCCAAGTAAGCTCCTAATCC`	`6 bp deleted → no frameshift`
564	`TCCATCCCCGGACTCTCCAGAGGGGGG-------AAAACCCCCAAGTAAGCTCCTAATCC`	`7 bp deleted → frameshift`
536	`TCCATCCCCGGACTCTCCAGAGGGGG--------AAAACCCCCAAGTAAGCTCCTAATCC`	`8 bp deleted → frameshift`
510	`TCCATCCCCGGACTCTCCAGAGGGG---------AAAACCCCCAAGTAAGCTCCTAATCC`	`9 bp deleted → no frameshift`
467	`TCCATCCCCGGACTCTCCAGAGGGGGGG-----GAAAACCCCCAAGTAAGCTCCTAATCC`	`5 bp deleted → frameshift`
364	`TCCATCCCCGGACTCTCCAGAGGG----------AAAACCCCCAAGTAAGCTCCTAATCC`	`10 bp deleted → frameshift`
327	`TCCATCCCCGGACTCTCCAGAGGGGGGG----GGAAAACCCCCAAGTAAGCTCCTAATCC`	`4 bp deleted → frameshift`
230	`TCCATCCCCGGACTCTCCAGAGG-----------AAAACCCCCAAGTAAGCTCCTAATCC`	`11 bp deleted → frameshift`
158	`TCCATCCCCGGA-----------------------AAACCCCCAAGTAAGCTCCTAATCC`	`23 bp deleted → frameshift`
153	`TCCATCCCC---------------------------------CAAGTAAGCTCCTAATCC`	`33 bp deleted → no frameshift`
149	`TCCATCCCCGGACTCTCCAGA--------------AAACCCCCAAGTAAGCTCCTAATCC`	`14 bp deleted → frameshift`
146	`TCCATCCCC----------------------------------AAGTAAGCTCCTAATCC`	`34 bp deleted → frameshift`
143	`TCCATCCCCGGACTCTCCA-------------------------AGTAAGCTCCTAATCC`	`25 bp deleted → frameshift`
140	`TCCATCCCCGG---------------------GGAAAACCCCCAAGTAAGCTCCTAATCC`	`21 bp deleted → no frameshift`
134	`TCCATCCCCGGACTCTCCAGAGGGGGGGAG----------------TAAGCTCCTAATCC`	`16 bp deleted → frameshift`
133	`TCCATCCCCGGACTCTCC----------------------CCCAAGTAAGCTCCTAATCC`	`22 bp deleted → frameshift`
133	`TCCATCCCCGG----------------------GAAAACCCCCAAGTAAGCTCCTAATCC`	`22 bp deleted → frameshift`
126	`TCCATCCCCGGACTCTCC-----------------------CCAAGTAAGCTCCTAATCC`	`23 bp deleted → frameshift`
121	`TCCATCCCC--------------------------------CCAAGTAAGCTCCTAATCC`	`32 bp deleted → frameshift`
120	`TCCATCCCCGGACTCTCC------------------------CAAGTAAGCTCCTAATCC`	`24 bp deleted → no frameshift`
115	`TCCATCCCCGGACTCTCC------------------------------------TAATCC`	`36 bp deleted → no frameshift`
110	`TCCATCCCCGGACTCTCCAGAGGGGGGGAG--------------------CTCCTAATCC`	`20 bp deleted → frameshift`
104	`TCCATCCC-----------------------------------AAGTAAGCTCCTAATCC`	`35 bp deleted → frameshift`
90	`TCCATCCCCGGACTCTCCAGAG------------------------TAAGCTCCTAATCC`	`24 bp deleted → no frameshift`
84	`TCCATCCCC-------------------------------CCCAAGTAAGCTCCTAATCC`	`31 bp deleted → frameshift`
81	`TCCATCCCCGGACTCTCCAG--------------------------TAAGCTCCTAATCC`	`26 bp deleted → frameshift`
81	`TCCATCCCCGGAC--------------------------CCCCAAGTAAGCTCCTAATCC`	`26 bp deleted → frameshift`
75	`TCCATCCCCGGACTC--------------------------------------CTAATCC`	`38 bp deleted → frameshift`
74	`TCCATCCCCGGACTCTCCAGAG----------------------------CTCCTAATCC`	`28 bp deleted → frameshift`
67	`TCCA----------------------------------------AGTAAGCTCCTAATCC`	`40 bp deleted → frameshift`
66	`TCCATCCCCGGACTCTCCAG------------------------------CTCCTAATCC`	`30 bp deleted → no frameshift`
66	`TCCATCC------------------------------------AAGTAAGCTCCTAATCC`	`36 bp deleted → no frameshift`
62	`TCC-------------------------------------CCCAAGTAAGCTCCTAATCC`	`37 bp deleted → frameshift`
61	`TCCATCCCCGGACTCTCC------------------------------------------`	`42 bp deleted → no frameshift`
60	`TCC--------------------------------------CCAAGTAAGCTCCTAATCC`	`38 bp deleted → frameshift`
56	`TCC---------------------------------------CAAGTAAGCTCCTAATCC`	`39 bp deleted → no frameshift`
47	`TCCATCC-----------------------------------------------TAATCC`	`47 bp deleted → frameshift`
42	`TCCATCCCCGGACTCT---------------------------------------AATCC`	`39 bp deleted → no frameshift`
42	`TCCATCC-----------------------------------------------------`	`53 bp deleted → frameshift`
42	`TCCATCCCC---------------------------------------------TAATCC`	`45 bp deleted → no frameshift`
40	`TCCATCCC----------------------------------------------TAATCC`	`46 bp deleted → frameshift`
39	`TCC---------------------------------------------------TAATCC`	`51 bp deleted → no frameshift`
38	`TCCATCCCCGGACT-----------------------------------------AATCC`	`41 bp deleted → frameshift`
33	`TCCATCCCCGGACTC--------------------------------------------C`	`44 bp deleted → frameshift`
31	`TCCATCCCC---------------------------------------------------`	`51 bp deleted → no frameshift`
29	`TCCATCCC----------------------------------------------------`	`52 bp deleted → frameshift`
29	`TCC---------------------------------------------------------`	`57 bp deleted → no frameshift`

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