CRISPOR

DNA break repair outcome predictions

Guide: GCGGACAGCCTCTCTGGCCA AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CGGGACCCCCAGTCCCCTCCGCGGACAGCCTCTCTGGCCAAGGGCAACCTAGTAGCTCAGACACCGAATCGGATTTCTAT`	(Wild-type sequence)
423	`AGTCCCCTCCGCGGACAGCCTCTCTGGC-------AACCTAGTAGCTCAGACACCGAATC`	`7 bp deleted → frameshift`
296	`AGTCCCCTCCGCGGACAGCCTCTCTGG------GCAACCTAGTAGCTCAGACACCGAATC`	`6 bp deleted → no frameshift`
242	`AGTCCCCTCCGCGGACAGCCTCTCTGGCC----------TAGTAGCTCAGACACCGAATC`	`10 bp deleted → frameshift`
222	`AGTCCCCTCCGCGGACAGCCTCTCTGGCCA------ACCTAGTAGCTCAGACACCGAATC`	`6 bp deleted → no frameshift`
193	`AGTCCCCTCCGCGGACAGCCT-------------------AGTAGCTCAGACACCGAATC`	`19 bp deleted → frameshift`
179	`AGTCCCCTCCGCGGACAGC----------------AACCTAGTAGCTCAGACACCGAATC`	`16 bp deleted → frameshift`
162	`AGTCCCCTCCGCGGACAGCCTCTCTGGC------------------TCAGACACCGAATC`	`18 bp deleted → no frameshift`
154	`AGTCCCCTCCGCGG-------------------GCAACCTAGTAGCTCAGACACCGAATC`	`19 bp deleted → frameshift`
150	`AGTCCCCTCCGCGGACAGCCTCTC------------------------AGACACCGAATC`	`24 bp deleted → no frameshift`
149	`AGTCCCCTCCGCGGACAG--------------GGCAACCTAGTAGCTCAGACACCGAATC`	`14 bp deleted → frameshift`
147	`AGTCCCCTCCGCGG--------------------CAACCTAGTAGCTCAGACACCGAATC`	`20 bp deleted → frameshift`
141	`AGTCCCCTCCGCGGACAGCCTCTCT---------------AGTAGCTCAGACACCGAATC`	`15 bp deleted → no frameshift`
136	`AGTCCCCTCCGCGGACAGCCTC--------------------------AGACACCGAATC`	`26 bp deleted → frameshift`
129	`AGTCCCCTCCGCGGACAGC---------------------------TCAGACACCGAATC`	`27 bp deleted → no frameshift`
128	`AGTCCCCTCCGCGGACAGCCTCT-----------------AGTAGCTCAGACACCGAATC`	`17 bp deleted → frameshift`
126	`AGTCCCCTCCGC-----------------------AACCTAGTAGCTCAGACACCGAATC`	`23 bp deleted → frameshift`
116	`AGTCCCCTCCGCGGACAGCCTCTCTGGCCA-------------------GACACCGAATC`	`19 bp deleted → frameshift`
116	`AGTCCCCTCCGCGGACA-------------------ACCTAGTAGCTCAGACACCGAATC`	`19 bp deleted → frameshift`
109	`AGTCCCCTCCGCGGACAGCCTCTCTGGCC--------------------------GAATC`	`26 bp deleted → frameshift`
101	`AGTCCCCTCCGCGGACAG--------------------------------ACACCGAATC`	`32 bp deleted → frameshift`
101	`AGTCCCCT--------------------------------AGTAGCTCAGACACCGAATC`	`32 bp deleted → frameshift`
99	`AGTCCCCTCCGCGGACAGCCTCTCT----------------------CAGACACCGAATC`	`22 bp deleted → frameshift`
99	`AGTCCCCTCCGCGGAC----------------------CTAGTAGCTCAGACACCGAATC`	`22 bp deleted → frameshift`
99	`AGTCCCCTCCGCGGACA------------------------------------CCGAATC`	`36 bp deleted → no frameshift`
95	`AGTCCCCTCCGCGGACAGCCTCTCTGGCCA-----------------------CCGAATC`	`23 bp deleted → frameshift`
94	`AGTCCCCTCC-----------------------------TAGTAGCTCAGACACCGAATC`	`29 bp deleted → frameshift`
90	`AGTCCCCTCCGCGGACAG------------------------TAGCTCAGACACCGAATC`	`24 bp deleted → no frameshift`
76	`AGTCCC---------------------------------TAGTAGCTCAGACACCGAATC`	`33 bp deleted → no frameshift`
73	`AGTCCCCTCCGC----------------------------------TCAGACACCGAATC`	`34 bp deleted → frameshift`
73	`AGTCC----------------------------------TAGTAGCTCAGACACCGAATC`	`34 bp deleted → frameshift`
71	`AGTCCCCTC---------------------------------------AGACACCGAATC`	`39 bp deleted → no frameshift`
69	`AGTCCCCTCCGCGGACAGCC-----------------------------------GAATC`	`35 bp deleted → frameshift`
66	`AG------------------------------GGCAACCTAGTAGCTCAGACACCGAATC`	`30 bp deleted → no frameshift`
63	`AGTCCCCTCCG---------------------------------------------AATC`	`45 bp deleted → no frameshift`
54	`AGT----------------------------------------AGCTCAGACACCGAATC`	`40 bp deleted → frameshift`
49	`AGTCCCCTCCGCGGACAGCCTCTC------------------------------------`	`36 bp deleted → no frameshift`
46	`AGTCCCCTCCGCG-------------------------------------------AATC`	`43 bp deleted → frameshift`
45	`AGTCCCCTCCGCGGACAGCCTC--------------------------------------`	`38 bp deleted → frameshift`
45	`AGTCCCCTCCGCGGAC--------------------------------------CGAATC`	`38 bp deleted → frameshift`
36	`AGTCCCCTCCGCGGA------------------------------------------ATC`	`42 bp deleted → no frameshift`
36	`AGTCCCC------------------------------------------------GAATC`	`48 bp deleted → no frameshift`
34	`AG-------------------------------------------CTCAGACACCGAATC`	`43 bp deleted → frameshift`
34	`AGTCCC-------------------------------------------------GAATC`	`49 bp deleted → frameshift`
33	`AGTC--------------------------------------------AGACACCGAATC`	`44 bp deleted → frameshift`
32	`AGTCC--------------------------------------------------GAATC`	`50 bp deleted → frameshift`
27	`AG------------------------------------------------ACACCGAATC`	`48 bp deleted → no frameshift`
23	`AGTCCCCTC---------------------------------------------------`	`51 bp deleted → no frameshift`
18	`AGTC--------------------------------------------------------`	`56 bp deleted → frameshift`

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