← return to the list of all guides
Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313825_F | Primer3: not found at this Tm | N.d. | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313825_R | Primer3: not found at this Tm | N.d. | 1.00 |
| mm4_intergenic_Vmn1r-ps54|Vmn1r74_chr7_11803445_F | Primer3: not found at this Tm | N.d. | 0.64 |
| mm4_intergenic_Vmn1r-ps54|Vmn1r74_chr7_11803445_R | Primer3: not found at this Tm | N.d. | 0.64 |
| mm4_intergenic_Rassf9|Alx1_chr10_102629836_F | TCGTCGGCAGCGTCACAAATAGGTGAGTGAGGCACA | 59.6 | 0.46 |
| mm4_intergenic_Rassf9|Alx1_chr10_102629836_R | GTCTCGTGGGCTCGGTGCCACATGCTTTTACTCACA | 58.4 | 0.46 |
| mm4_intron_Nxn_chr11_76354611_F | TCGTCGGCAGCGTCGCACCACTCCATCTCACCAA | 59.9 | 0.42 |
| mm4_intron_Nxn_chr11_76354611_R | GTCTCGTGGGCTCGGAGGTGGTAGGAAGGACCCTG | 60.2 | 0.42 |
| mm3_exon_Nynrin/Gm26751_chr14_55871434_F | TCGTCGGCAGCGTCTGCAACTGGATCTTCAGCGT | 59.9 | 0.41 |
| mm3_exon_Nynrin/Gm26751_chr14_55871434_R | GTCTCGTGGGCTCGGGATGAGGTAGTGAAGCCCCG | 59.8 | 0.41 |
| mm4_intron_G930045G22Rik_chr6_50871221_F | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4_intron_G930045G22Rik_chr6_50871221_R | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4_intron_Lurap1l_chr4_80947602_F | TCGTCGGCAGCGTCGACAGCAGTGTCCTTGTGGA | 59.8 | 0.38 |
| mm4_intron_Lurap1l_chr4_80947602_R | GTCTCGTGGGCTCGGACCACAGGAGATTCTCTGCC | 59.0 | 0.38 |
| mm4_intergenic_Ifng|Dyrk2/4932442E05Rik_chr10_118819427_F | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intergenic_Ifng|Dyrk2/4932442E05Rik_chr10_118819427_R | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intron_Wdr45_chrX_7725626_F | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intron_Wdr45_chrX_7725626_R | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_exon_Cd247_chr1_165861240_F | TCGTCGGCAGCGTCTGGTTCTCTCACTTGCCACA | 59.1 | 0.29 |
| mm4_exon_Cd247_chr1_165861240_R | GTCTCGTGGGCTCGGCCAGAAGACCCAAGAGCAGG | 60.0 | 0.29 |
| mm4_intron_Cabin1_chr10_75660449_F | TCGTCGGCAGCGTCAAGCATCCACACCTGTACCG | 60.0 | 0.28 |
| mm4_intron_Cabin1_chr10_75660449_R | GTCTCGTGGGCTCGGGGGAGGAGATCTGGAGGTCA | 59.7 | 0.28 |
| mm3_exon_Bcar1_chr8_111713396_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm3_exon_Bcar1_chr8_111713396_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Cdh8|Gm15680_chr8_99294886_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Cdh8|Gm15680_chr8_99294886_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_n-R5s209|Gm7846_chr1_28215576_F | TCGTCGGCAGCGTCTTGCTTTGTAGGCATGGCAG | 59.1 | 0.26 |
| mm4_intergenic_n-R5s209|Gm7846_chr1_28215576_R | GTCTCGTGGGCTCGGTGCTATCCAACTTGCTTTTGG | 57.0 | 0.26 |
| mm3_intergenic_Med13l|n-R5s175_chr5_119088555_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm3_intergenic_Med13l|n-R5s175_chr5_119088555_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm3_intergenic_n-R5s42|Fam19a5_chr15_87215595_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm3_intergenic_n-R5s42|Fam19a5_chr15_87215595_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm3_intergenic_4930552P12Rik|Tcf7l2_chr19_55733090_F | TCGTCGGCAGCGTCGGAGAGGAACAGGGTGAGGA | 60.2 | 0.23 |
| mm3_intergenic_4930552P12Rik|Tcf7l2_chr19_55733090_R | GTCTCGTGGGCTCGGGCTGTCGCTTTTCCTAGGGT | 60.0 | 0.23 |
| mm4_intergenic_Hmg20a|Gm26868_chr9_56503737_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Hmg20a|Gm26868_chr9_56503737_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm3_intron_Sufu_chr19_46477782_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm3_intron_Sufu_chr19_46477782_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Ppp1r15b|Plekha6_chr1_133160322_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Ppp1r15b|Plekha6_chr1_133160322_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Sept8_chr11_53536569_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Sept8_chr11_53536569_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Cers3|Adamts17_chr7_66835373_F | TCGTCGGCAGCGTCGACCATGGAATCAAGCCGGA | 60.1 | 0.21 |
| mm4_intergenic_Cers3|Adamts17_chr7_66835373_R | GTCTCGTGGGCTCGGTGGGGCTATCTGAGGGTGAA | 59.9 | 0.21 |
| mm4_intergenic_Gm23905|Zfp36l2_chr17_84000654_F | TCGTCGGCAGCGTCTGACTCCAGATGATGTGGGA | 57.7 | 0.21 |
| mm4_intergenic_Gm23905|Zfp36l2_chr17_84000654_R | GTCTCGTGGGCTCGGGGGTAACAGGTGGGATAGGC | 59.5 | 0.21 |
| mm4_exon_Gm5205_chr14_40557805_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_exon_Gm5205_chr14_40557805_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Arhgap24_chr5_102518229_F | TCGTCGGCAGCGTCGGGTTGGTTAGGCTCATTAGACA | 60.0 | 0.20 |
| mm4_intron_Arhgap24_chr5_102518229_R | GTCTCGTGGGCTCGGACCAGCACAGGAGATGAAGC | 60.0 | 0.20 |
| mm4_intergenic_Gm12189|Olfr1389_chr11_49429660_F | TCGTCGGCAGCGTCGCTAGAGCAGAACCATTACAAAGA | 58.8 | 0.19 |
| mm4_intergenic_Gm12189|Olfr1389_chr11_49429660_R | GTCTCGTGGGCTCGGTTTTGCAAGTCACTATTTCCCATT | 57.5 | 0.19 |
| mm3_intergenic_Rab17|Lrrfip1_chr1_90972880_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm3_intergenic_Rab17|Lrrfip1_chr1_90972880_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Zcchc6|Gas1_chr13_60014352_F | TCGTCGGCAGCGTCGCCAGCTGGATGAACAATGC | 60.1 | 0.19 |
| mm4_intergenic_Zcchc6|Gas1_chr13_60014352_R | GTCTCGTGGGCTCGGAGCAGTGTCCAAGTCTCTCG | 59.3 | 0.19 |
| mm4_intergenic_Tnfrsf13c|Cenpm_chr15_82230538_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Tnfrsf13c|Cenpm_chr15_82230538_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Cenpw|Gm22623_chr10_30436091_F | TCGTCGGCAGCGTCACTCAAGTTGGCATCTGGTGT | 59.8 | 0.18 |
| mm4_intergenic_Cenpw|Gm22623_chr10_30436091_R | GTCTCGTGGGCTCGGCTGATCCCTCCTGCAGCAAG | 60.4 | 0.18 |
| mm4_intron_Prkch_chr12_73678244_F | TCGTCGGCAGCGTCGCCGTGTGAAGCAATCCATAC | 59.9 | 0.18 |
| mm4_intron_Prkch_chr12_73678244_R | GTCTCGTGGGCTCGGCCTCATCCACTTCCATCAGGA | 58.8 | 0.18 |
| mm4_intergenic_Ppm1h|Mirlet7i_chr10_122966525_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Ppm1h|Mirlet7i_chr10_122966525_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm24462|C230034O21Rik_chr3_38703238_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm24462|C230034O21Rik_chr3_38703238_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_A430078I02Rik|Pcbp1_chr6_86499930_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_A430078I02Rik|Pcbp1_chr6_86499930_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Elp4_chr2_105751874_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Elp4_chr2_105751874_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Dync1i1_chr6_5814265_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Dync1i1_chr6_5814265_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Cytip|Acvr1c_chr2_58240792_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Cytip|Acvr1c_chr2_58240792_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Hecw1|Mrpl32_chr13_14563161_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Hecw1|Mrpl32_chr13_14563161_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Hddc3|Man2a2_chr7_80346700_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Hddc3|Man2a2_chr7_80346700_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Sult2a7_chr7_14478469_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Sult2a7_chr7_14478469_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm9341|Gm24444_chr7_136320174_F | TCGTCGGCAGCGTCGGGGAGACTGAGGAATGCAC | 60.1 | 0.14 |
| mm4_intergenic_Gm9341|Gm24444_chr7_136320174_R | GTCTCGTGGGCTCGGTGGAAGTGCAATATGGCCCA | 59.6 | 0.14 |
| mm4_intron_Gpr125_chr5_50053753_F | TCGTCGGCAGCGTCCAAGACTCCACCCCGATGAC | 60.1 | 0.14 |
| mm4_intron_Gpr125_chr5_50053753_R | GTCTCGTGGGCTCGGTGGGAAGAGGTGGGGAAGAA | 60.1 | 0.14 |
| mm4_intergenic_Gm9843|Usp25_chr16_76470165_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm9843|Usp25_chr16_76470165_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm24404|Nrg3_chr14_38072313_F | TCGTCGGCAGCGTCAACCCTACTAAGTTGCCCCA | 58.2 | 0.14 |
| mm4_intergenic_Gm24404|Nrg3_chr14_38072313_R | GTCTCGTGGGCTCGGAGGCCAGCCAAAAGTAGGAC | 59.9 | 0.14 |
| mm4_intergenic_Gm12184/Trim7|Trim7_chr11_48834423_F | TCGTCGGCAGCGTCACTATGCTAAAAGGTAGGCGGT | 59.2 | 0.14 |
| mm4_intergenic_Gm12184/Trim7|Trim7_chr11_48834423_R | GTCTCGTGGGCTCGGAGGGCATTTCTGAGCGTACT | 59.0 | 0.14 |
| mm4_intergenic_Gm24049|Vav2_chr2_27407801_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm24049|Vav2_chr2_27407801_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Zbtb7c_chr18_76036233_F | TCGTCGGCAGCGTCTCACATGGAGAAAGGCAGCC | 60.3 | 0.13 |
| mm4_intron_Zbtb7c_chr18_76036233_R | GTCTCGTGGGCTCGGCACGGGGAGATTGCTGTTTG | 59.4 | 0.13 |
| mm4_intergenic_Rhobtb2|Pebp4_chr14_69837091_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Rhobtb2|Pebp4_chr14_69837091_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Etfa_chr9_55482901_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Etfa_chr9_55482901_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm2_intergenic_Ccdc130|Gm23626_chr8_84362613_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm2_intergenic_Ccdc130|Gm23626_chr8_84362613_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Ctnna3|Gm10118_chr10_63881670_F | TCGTCGGCAGCGTCAAGTGTGGCAGGAGTCCTTG | 59.8 | 0.12 |
| mm4_intergenic_Ctnna3|Gm10118_chr10_63881670_R | GTCTCGTGGGCTCGGAGGAAAGCTGGAGCAGATGG | 59.7 | 0.12 |
| mm4_intron_Tenm3_chr8_48262677_F | TCGTCGGCAGCGTCCCAGAGAAGGCAGCAGTCTG | 60.3 | 0.12 |
| mm4_intron_Tenm3_chr8_48262677_R | GTCTCGTGGGCTCGGGTGTGAGAGGAAGGTCTGCC | 60.0 | 0.12 |
| mm4_intergenic_Wfdc18|1100001G20Rik_chr11_83711395_F | TCGTCGGCAGCGTCTCCTTAGCATCCTTCCTGTTGA | 58.8 | 0.12 |
| mm4_intergenic_Wfdc18|1100001G20Rik_chr11_83711395_R | GTCTCGTGGGCTCGGAGGGCTCTGTCTGTGTTTATGG | 60.0 | 0.12 |
| mm4_intergenic_Wwc1|Gm12125_chr11_35985201_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Wwc1|Gm12125_chr11_35985201_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Zfr2_chr10_81236746_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Zfr2_chr10_81236746_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Lrrc48_chr11_60382342_F | TCGTCGGCAGCGTCCCATTCGCACCTTCTGAGGA | 59.7 | 0.11 |
| mm4_intron_Lrrc48_chr11_60382342_R | GTCTCGTGGGCTCGGCAGCTTCTTGTGGGCTGAGA | 59.9 | 0.11 |
| mm4_exon_C2cd2_chr16_97892305_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_exon_C2cd2_chr16_97892305_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm13602|Gm13598_chr2_66977513_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm13602|Gm13598_chr2_66977513_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3_intron_Plxnb1_chr9_109115131_F | TCGTCGGCAGCGTCGGCACAGATCTTCAGGGCTT | 60.0 | 0.11 |
| mm3_intron_Plxnb1_chr9_109115131_R | GTCTCGTGGGCTCGGCTGAGACTGACCACGGCTA | 58.4 | 0.11 |
| mm4_intron_Cntnap5a_chr1_116050723_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Cntnap5a_chr1_116050723_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Ablim2_chr5_35794655_F | TCGTCGGCAGCGTCGCATAGGCCTTAGGGTTGCA | 60.1 | 0.10 |
| mm4_intron_Ablim2_chr5_35794655_R | GTCTCGTGGGCTCGGGTAACCTGAGGCCAAGAGCA | 59.6 | 0.10 |
| mm4_exon_Gpr179_chr11_97347465_F | TCGTCGGCAGCGTCATAGAATCCAGGTCGGCAGC | 59.6 | 0.10 |
| mm4_exon_Gpr179_chr11_97347465_R | GTCTCGTGGGCTCGGCCACAGCACGGAAAGCTTTC | 60.0 | 0.10 |
| mm3_intergenic_1700036O09Rik|Gm14689_chrX_67473959_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm3_intergenic_1700036O09Rik|Gm14689_chrX_67473959_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Vmn2r60_chr7_42166972_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Vmn2r60_chr7_42166972_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Vmn2r60|Vmn2r61_chr7_42201791_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Vmn2r60|Vmn2r61_chr7_42201791_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm8919|Gm8935_chr3_11774957_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm8919|Gm8935_chr3_11774957_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Atp7b_chr8_21997164_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Atp7b_chr8_21997164_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Dab1_chr4_104536564_F | TCGTCGGCAGCGTCAGGAAAGCGAGAACTGTGCA | 59.8 | 0.08 |
| mm4_intron_Dab1_chr4_104536564_R | GTCTCGTGGGCTCGGTTTCAGCTGGCCTCTGTCTG | 59.9 | 0.08 |
| mm3_intergenic_Rxrb|Col11a2_chr17_34039129_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intergenic_Rxrb|Col11a2_chr17_34039129_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Lsamp_chr16_42133975_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Lsamp_chr16_42133975_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Vrk1|Gm24986_chr12_106199160_F | TCGTCGGCAGCGTCCCCCAGTTAGGTGAGACTGC | 59.7 | 0.08 |
| mm4_intergenic_Vrk1|Gm24986_chr12_106199160_R | GTCTCGTGGGCTCGGTTGTGTCCTGTACCGTGGTG | 59.8 | 0.08 |
| mm4_intergenic_Slco3a1|Gm7580_chr7_74369831_F | TCGTCGGCAGCGTCGGTCCCCTTTGGTTTCCCAT | 59.8 | 0.08 |
| mm4_intergenic_Slco3a1|Gm7580_chr7_74369831_R | GTCTCGTGGGCTCGGTCACTGCTTTGCTTCACTGAC | 59.0 | 0.08 |
| mm3_intron_Hmcn1_chr1_150619441_F | TCGTCGGCAGCGTCTGATTCACTTTGGGCACTTCT | 57.7 | 0.07 |
| mm3_intron_Hmcn1_chr1_150619441_R | GTCTCGTGGGCTCGGCTCTGCTGAGACATCGTGACA | 59.7 | 0.07 |
| mm4_intron_Zbtb16_chr9_48795903_F | TCGTCGGCAGCGTCTTCTCTTAGCCCCAGCCTCT | 59.9 | 0.07 |
| mm4_intron_Zbtb16_chr9_48795903_R | GTCTCGTGGGCTCGGGCCCACTGTTAGGTCTTGCT | 59.9 | 0.07 |
| mm4_intron_Osbpl10_chr9_115213442_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Osbpl10_chr9_115213442_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm22593|Mettl4_chr17_94706024_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm22593|Mettl4_chr17_94706024_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Ttc29_chr8_78265035_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Ttc29_chr8_78265035_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intergenic_Capn8|4922505E12Rik_chr1_182728006_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intergenic_Capn8|4922505E12Rik_chr1_182728006_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm5742|Gm22223_chr8_101418239_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm5742|Gm22223_chr8_101418239_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_exon_4930563D23Rik_chr16_92321425_F | TCGTCGGCAGCGTCGTTTGTGAAGCGAGCCATGG | 60.1 | 0.07 |
| mm4_exon_4930563D23Rik_chr16_92321425_R | GTCTCGTGGGCTCGGGCCCCTGACACGTAGCTATG | 60.2 | 0.07 |
| mm4_intron_Tshr_chr12_91512047_F | TCGTCGGCAGCGTCACTGGGTAACTGCCACCATC | 59.6 | 0.07 |
| mm4_intron_Tshr_chr12_91512047_R | GTCTCGTGGGCTCGGCCAGATGGCCCTTGTCAAGT | 59.9 | 0.07 |
| mm4_intergenic_Olfr1219|Olfr1220_chr2_89093618_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Olfr1219|Olfr1220_chr2_89093618_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Cyp3a59|Gm6309_chr5_146159220_F | TCGTCGGCAGCGTCAATCGCCCTACCCCACTGTA | 60.3 | 0.07 |
| mm4_intergenic_Cyp3a59|Gm6309_chr5_146159220_R | GTCTCGTGGGCTCGGTGGTAAGATGGGGTAGGGGA | 58.9 | 0.07 |
| mm4_intron_Gm6408_chr5_146483250_F | TCGTCGGCAGCGTCACGGAGGTAAGAGCCCATCT | 60.0 | 0.06 |
| mm4_intron_Gm6408_chr5_146483250_R | GTCTCGTGGGCTCGGGGCCATTAACTCCCTCTTCCC | 60.1 | 0.06 |
| mm4_intergenic_Lmnb2|Gadd45b_chr10_80926821_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Lmnb2|Gadd45b_chr10_80926821_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_March1_chr8_66241592_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_March1_chr8_66241592_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_1810041L15Rik|Ldoc1l_chr15_84526582_F | TCGTCGGCAGCGTCGGCCAGGGGTGGACTATACT | 60.4 | 0.06 |
| mm4_intergenic_1810041L15Rik|Ldoc1l_chr15_84526582_R | GTCTCGTGGGCTCGGAAAGCAGGTTCCTTGACCCC | 60.1 | 0.06 |
| mm4_intron_Antxr1_chr6_87158190_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Antxr1_chr6_87158190_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_4932435O22Rik|Gm11689_chr11_114657392_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_4932435O22Rik|Gm11689_chr11_114657392_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intron_Klf8_chrX_153293911_F | TCGTCGGCAGCGTCAGAAACTGGTGGTGAAAGAATCA | 58.2 | 0.05 |
| mm3_intron_Klf8_chrX_153293911_R | GTCTCGTGGGCTCGGTGAGACTCAAAATATTGAAACTGCT | 57.2 | 0.05 |
| mm4_intron_Tcf4_chr18_69613175_F | TCGTCGGCAGCGTCCGATCTAAATGAGGAGGAATCAGC | 59.0 | 0.05 |
| mm4_intron_Tcf4_chr18_69613175_R | GTCTCGTGGGCTCGGCTGGCAACTGTAGGAAGGGG | 60.0 | 0.05 |
| mm4_intergenic_Syngr2|Tk1_chr11_117815338_F | TCGTCGGCAGCGTCCCCCATGCCAGCTAGAAACA | 60.0 | 0.05 |
| mm4_intergenic_Syngr2|Tk1_chr11_117815338_R | GTCTCGTGGGCTCGGGTCTCACCACAGCCCAAGAA | 59.8 | 0.05 |
| mm4_intergenic_Lpl|Slc18a1_chr8_68916573_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Lpl|Slc18a1_chr8_68916573_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Fgf13_chrX_59433870_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Fgf13_chrX_59433870_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm14561|Gm6071_chrX_22235595_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm14561|Gm6071_chrX_22235595_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Gm13474_chr2_38976514_F | TCGTCGGCAGCGTCTCACTGTGCCTGCTTCCTTT | 59.8 | 0.05 |
| mm4_exon_Gm13474_chr2_38976514_R | GTCTCGTGGGCTCGGCGCAGCTGTCCCATTAGACT | 59.8 | 0.05 |
| mm4_intergenic_Gm22157|Hivep1_chr13_41958744_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm22157|Hivep1_chr13_41958744_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Farp1_chr14_121276888_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Farp1_chr14_121276888_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Sil1_chr18_35384221_F | TCGTCGGCAGCGTCCCCAGCTCGCTCCTATTAGC | 60.0 | 0.04 |
| mm4_intron_Sil1_chr18_35384221_R | GTCTCGTGGGCTCGGAGCTGCAGTGTAGTTGGGAG | 59.6 | 0.04 |
| mm4_intron_Megf11_chr9_64389752_F | TCGTCGGCAGCGTCGGGACTCCCAGGACACTCTT | 60.5 | 0.04 |
| mm4_intron_Megf11_chr9_64389752_R | GTCTCGTGGGCTCGGTCTCTGTGTGTGTGCTGATGA | 59.3 | 0.04 |
| mm4_intergenic_Gstm5|Gm12489_chr3_107899454_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gstm5|Gm12489_chr3_107899454_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Cxcl1_chr5_90892426_F | TCGTCGGCAGCGTCAGAACTCTGGGTGCAAGTGT | 59.1 | 0.03 |
| mm4_intron_Cxcl1_chr5_90892426_R | GTCTCGTGGGCTCGGCCGTTACTTGGGGACACCTC | 60.0 | 0.03 |
| mm3_intron_Ankdd1b_chr13_96434631_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intron_Ankdd1b_chr13_96434631_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm22507|Trhde_chr10_114071766_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm22507|Trhde_chr10_114071766_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intron_Myzap/Gcom1_chr9_71550960_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intron_Myzap/Gcom1_chr9_71550960_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Gm25577|Gm22537_chr12_118542876_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Gm25577|Gm22537_chr12_118542876_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm11369|Uqcrfs1_chr13_30452255_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm11369|Uqcrfs1_chr13_30452255_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Cplx1|Gak_chr5_108552068_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Cplx1|Gak_chr5_108552068_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm24092|Cenpf_chr1_189490276_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm24092|Cenpf_chr1_189490276_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Tesc_chr5_118039478_F | TCGTCGGCAGCGTCGCACGGGCTGATGTCTGTAT | 60.1 | 0.02 |
| mm4_intron_Tesc_chr5_118039478_R | GTCTCGTGGGCTCGGCAGCAGCCACTACAACCTGA | 59.9 | 0.02 |
| mm4_intron_Lhfp_chr3_53168683_F | TCGTCGGCAGCGTCGCGTGTACATCTGCTTTGTGA | 59.2 | 0.02 |
| mm4_intron_Lhfp_chr3_53168683_R | GTCTCGTGGGCTCGGGGAGGTCCTTGTGGAGAACTG | 59.9 | 0.02 |
| mm3_intergenic_Pcgf5|Gm23170_chr19_36381831_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Pcgf5|Gm23170_chr19_36381831_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Slc28a3|Ntrk2_chr13_58714932_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Slc28a3|Ntrk2_chr13_58714932_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intron_Havcr1_chr11_46736728_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intron_Havcr1_chr11_46736728_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_exon_Tnfaip2_chr12_111445096_F | TCGTCGGCAGCGTCCCGAGGCTGAATCAGAGACC | 59.8 | 0.02 |
| mm3_exon_Tnfaip2_chr12_111445096_R | GTCTCGTGGGCTCGGTGAACATGTTGGCCAGTCCT | 59.5 | 0.02 |
| mm4_exon_Sntb1_chr15_55906325_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Sntb1_chr15_55906325_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Calml4_chr9_62871012_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Calml4_chr9_62871012_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_2410088K16Rik|Sh3bp4_chr1_89014498_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_2410088K16Rik|Sh3bp4_chr1_89014498_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm16029/Egflam|Gm16029_chr15_7405104_F | TCGTCGGCAGCGTCTGTAGGCACACACATCACAT | 57.1 | 0.01 |
| mm4_intergenic_Gm16029/Egflam|Gm16029_chr15_7405104_R | GTCTCGTGGGCTCGGCTGCTGTGTGGGTAGACTGG | 60.0 | 0.01 |
| mm4_intron_Hivep3_chr4_119993170_F | TCGTCGGCAGCGTCGGACTGCTCACTGTCGAGTC | 60.1 | 0.01 |
| mm4_intron_Hivep3_chr4_119993170_R | GTCTCGTGGGCTCGGAGCTTGCATCCATCTCACCC | 60.1 | 0.01 |
| mm4_intron_Gsg1l_chr7_125886054_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Gsg1l_chr7_125886054_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Nfat5_chr8_107294013_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Nfat5_chr8_107294013_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Prkch|Hif1a_chr12_73815232_F | TCGTCGGCAGCGTCAGCTGACCTCCTGGCTCATA | 60.0 | 0.01 |
| mm3_intergenic_Prkch|Hif1a_chr12_73815232_R | GTCTCGTGGGCTCGGTCAGACAACTCGGCATTGCT | 59.9 | 0.01 |
| mm3_intron_Ezr_chr17_6758120_F | TCGTCGGCAGCGTCCTCTGCTGCTGTAGAGCCAC | 60.4 | 0.01 |
| mm3_intron_Ezr_chr17_6758120_R | GTCTCGTGGGCTCGGAGGGAATTCCAGCACCTTCG | 60.0 | 0.01 |
| mm4_intron_P2rx5_chr11_73170558_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_P2rx5_chr11_73170558_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Wscd2_chr5_113497178_F | TCGTCGGCAGCGTCAATGGGATGGGGCTTCTCAG | 59.4 | 0.00 |
| mm4_intron_Wscd2_chr5_113497178_R | GTCTCGTGGGCTCGGCAGGCAAGGCTAGAAGGGAC | 60.1 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| mm4_intergenic_Rassf9|Alx1_chr10_102629836 |
ACAAATAGGTGAGTGAGGCACACCTGTGAAACATCCCAGGAATCTACAGAAGGGTCACAACTTTCTTTCCCATCACAGGA ATGTGAGAGATATGCAAATGTGAGTAAAAGCATGTGGCA |
| mm4_intron_Nxn_chr11_76354611 |
GCACCACTCCATCTCACCAAGTTTCCTTTCTCTTCACCGGGCTCTCCAGAAGGCGCCCTGGCCAGCAAGCTGCTCCTCAG GGAACCCAATACAGGGTCCTTCCTACCACCT |
| mm3_exon_Nynrin/Gm26751_chr14_55871434 |
TGCAACTGGATCTTCAGCGTTCTCTGGGAGCTCCTGCCCCTCTGGAGAGTCCGGGGCTTCCTGTCATCTGACGGGGCTTC ACTACCTCATC |
| mm4_intron_Lurap1l_chr4_80947602 |
GACAGCAGTGTCCTTGTGGATCTGCTAAGAAAGGCAGATAGGGAGACATGCTGTATAATGCAGCTGTCAATAAACCTTCT GGAGAATCCAAGGATGAGTAGGCAGAGAATCTCCTGTGGT |
| mm4_exon_Cd247_chr1_165861240 |
TGGTTCTCTCACTTGCCACATTTGTCTTCTTCAGTTCCAGAGCACTGAACACAGAACGTCATCCCTGGACTCTCTAAAGG GAGAGCCACCCTTGCTCTTCCACCCCAGCCCTGCTCTTGGGTCTTCTGG |
| mm4_intron_Cabin1_chr10_75660449 |
AAGCATCCACACCTGTACCGAGCTCCACTGCTGGCAGCAGCTCTTCCGTCTGGAAGGTCAGGAGATGGATGCCTTGGGTC AGTCACCCTGATGACCAGATGCTCTCCTTGACCTCCAGATCTCCTCCC |
| mm4_intergenic_n-R5s209|Gm7846_chr1_28215576 |
TTGCTTTGTAGGCATGGCAGCCTAAATGTAATTCCATCTGTAGAGTCTAGGGTTGGGAGATTCCAAAAGCAAGTTGGATA GCA |
| mm3_intergenic_4930552P12Rik|Tcf7l2_chr19_55733090 |
GGAGAGGAACAGGGTGAGGACCTAGGCTTCCCAGGTGCCAGGACTTAGGTAGTCCTGGCATTCCAGGCCCTCTGGAGAGT CCAGGAAGGGAAAGGGAACTCACCCTAGGAAAAGCGACAGC |
| mm4_intergenic_Cers3|Adamts17_chr7_66835373 |
GACCATGGAATCAAGCCGGAAGAACAAAAGAGAGTCAAAGTGATGGGCTTCCCCACTTTTATTCTTGGCAAGCTTCCTTC TGGAGGCTCCAGGGAAGGAGAATTCGATAGCCATGTAACTTCACCCTCAGATAGCCCCA |
| mm4_intergenic_Gm23905|Zfp36l2_chr17_84000654 | TGACTCCAGATGATGTGGGACCCTCTGGAGAGTCAGGGTATCAGAACTCAATATATGAGCCTATCCCACCTGTTACCC |
| mm4_intron_Arhgap24_chr5_102518229 |
GGGTTGGTTAGGCTCATTAGACAGTCAGTTAATAACATACTCATTTAAATAGTCATTCTCCCTCAACACACGGACTCTCC AGATGGCTTGCTGCTATATACATTAGCAACAGTGACATTGCTTCATCTCCTGTGCTGGT |
| mm4_intergenic_Gm12189|Olfr1389_chr11_49429660 |
GCTAGAGCAGAACCATTACAAAGAGTTTTTTCTAGAGAGGAAAAAAAATAACTCCTCCTGGAAAGGCCAGGGATGGATTT CCCAGCTACATTTTTTAAGTGAAATATAATGGGAAATAGTGACTTGCAAAA |
| mm4_intergenic_Zcchc6|Gas1_chr13_60014352 |
GCCAGCTGGATGAACAATGCTTATGTTTTGAAAGCACTGTGTCCTCTGTAGGATAATGTGGGCTTTGCGAAAACTCCATG CCCAGGCTCTCCAGGGGGCGAGAGACTTGGACACTGCT |
| mm4_intergenic_Cenpw|Gm22623_chr10_30436091 |
ACTCAAGTTGGCATCTGGTGTTTTCATTAAGGTGTGCCTTTATAGAAGCAGGGACAGTGATATTCTGATGCCAGGCTAAG TCCTCCTGGAGAGGCCAGGGAAGGGGAGGAAGCCTTGCACTTGCTGCAGGAGGGATCAG |
| mm4_intron_Prkch_chr12_73678244 |
GCCGTGTGAAGCAATCCATACCTTTGTCAACAGAGGAATTTTATCAGAAGAAAACATGGAAAGTTTTTGTGTGACAGTAT TGGACTCTTTCCCTTCTGGTGAGTCAGGGCATGATCCTGATGGAAGTGGATGAGG |
| mm4_intergenic_Gm9341|Gm24444_chr7_136320174 |
GGGGAGACTGAGGAATGCACATGGCACCGTCTGAAGAGTCAGGGGATATTAAGGAAGGGAAGAAAATCAGAGCAGAGATT CCTTTTGGGCCATATTGCACTTCCA |
| mm4_intron_Gpr125_chr5_50053753 |
CAAGACTCCACCCCGATGACATTATGCAACCCCCCCCCCCCATGCCATGACTCTCCAAAAGGGCCCTTCTTCCCCACCTC TTCCCA |
| mm4_intergenic_Gm24404|Nrg3_chr14_38072313 |
AACCCTACTAAGTTGCCCCATATGGGACCTATTTCTTCTGGATACCATCCAAGGCCTTTCCAGATGGTCCTTCCTCTTTT ATCCCATGTCACCATTGTTACTGTCCTACTTTTGGCTGGCCT |
| mm4_intergenic_Gm12184/Trim7|Trim7_chr11_48834423 |
ACTATGCTAAAAGGTAGGCGGTAGCCGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNACTCACTGTTACAGTACGCTCAGAAATGCCCT |
| mm4_intron_Zbtb7c_chr18_76036233 |
TCACATGGAGAAAGGCAGCCATATATACCCCAGAGACTCCCTGCTGTCCCCTGACTCTCCACATGGCTAGCTCACTTTCT CACTTATCCCAAAGCTGCACTTGCAAACAGCAATCTCCCCGTG |
| mm4_intergenic_Ctnna3|Gm10118_chr10_63881670 |
AAGTGTGGCAGGAGTCCTTGGAAATGGAAGGTACCATCAGTGCACACTGCTGCCATAGCCTTCTGAAGAGTCCTGGGAAA GGTGTGCAGAGCCCCATCTGCTCCAGCTTTCCT |
| mm4_intron_Tenm3_chr8_48262677 |
CCAGAGAAGGCAGCAGTCTGCAGGTACCTTGCCAGTCTCTCTACCCCTGAACTCTCCAGACAGCATGAGTTCAGAGGACG TGGACAAGGGCAGACCTTCCTCTCACAC |
| mm4_intergenic_Wfdc18|1100001G20Rik_chr11_83711395 |
TCCTTAGCATCCTTCCTGTTGAAGAGTTCTTATTTGTGTATCATATTGACTATTTTATTCACAAGAGCCATCCCTGCAGC CTCCAGAGGGTCCATAAACACAGACAGAGCCCT |
| mm4_intron_Lrrc48_chr11_60382342 |
CCATTCGCACCTTCTGAGGACCAGGCTGGGGCCTGACTCACCCTCGAAACAAGTCCCTCTGGAGAGGCAGGTCATGGACC CCGCTAAACCTGACTCAGTGCCCTGCCTGTATCTCAGCCCACAAGAAGCTG |
| mm3_intron_Plxnb1_chr9_109115131 |
GGCACAGATCTTCAGGGCTTCCTCAGAGTTGCAGGGAGAAGATAGTCTGTGGGCAGCCTCTGACTTAAGGCCTTCCCACT CCAGGCCTCATCCCAGGGCTCTCCAGAAAGTAGCCGTGGTCAGTCTCAG |
| mm4_intron_Ablim2_chr5_35794655 |
GCATAGGCCTTAGGGTTGCACCATGCAGGCCCCCCAGAACCCTCTCCTGACTCTCCTGATGGTGCTTCCAAGCCTTGGCA TGGCTGGCCTGGGCCCATTCAGCTGCTCTTGGCCTCAGGTTAC |
| mm4_exon_Gpr179_chr11_97347465 |
ATAGAATCCAGGTCGGCAGCGGCAGAGATAGCGGCCAAGGACAAAACCCTGACTCTCCAGAGGGACACACTGTGGGGATG ACAAATGACATTTGTTTGTATGATGTTTTTCTCCGAAAGCTTTCCGTGCTGTGG |
| mm4_intron_Dab1_chr4_104536564 |
AGGAAAGCGAGAACTGTGCATAACTCAAACAGTGGCCAGTCTCTTGCAAGCCCTCCTTCTGGTGATTGGGGGGATGGATT TGGGGCATCATGTCAGGGGCTCTCAATTCAGACAGAGGCCAGCTGAAA |
| mm4_intergenic_Vrk1|Gm24986_chr12_106199160 |
CCCCAGTTAGGTGAGACTGCTCCCCATGTGGAGTGTCTGGGGAGGGGTGGGTGTAGGAGAGTCCCCCGCAGCACCACGGT ACAGGACACAA |
| mm4_intergenic_Slco3a1|Gm7580_chr7_74369831 |
GGTCCCCTTTGGTTTCCCATTGTCCATACCCGCAGCCTCCAGAAGGGGGCAACTGAGAGACAGAAAGACCTAAAGTGGGC CATTTAGTGGCAAGGCCAATAAGTAGTCTTAGCAGTAGTCAGTGAAGCAAAGCAGTGA |
| mm3_intron_Hmcn1_chr1_150619441 |
TGATTCACTTTGGGCACTTCTAAAATGGATTCCATCAAGCTTTGTGAAATACTTCATTGCTCAAAGATACATCCGATCCC CGTATTCTCCAGAGAGCTTGTCACGATGTCTCAGCAGAG |
| mm4_intron_Zbtb16_chr9_48795903 |
TTCTCTTAGCCCCAGCCTCTAGCCATCAATAACCACAGTGCTGTGCTTCACAGTGAAAATAATGATCAATACAATGACGT GCCATCTGGTGACTCCGGCCATGGAGAAAAGCAAGACCTAACAGTGGGC |
| mm4_exon_4930563D23Rik_chr16_92321425 |
GTTTGTGAAGCGAGCCATGGGGCTCCCTGAGTGGCTCACATTTTCCCATCTGGGGAGTTCTGGGAGGGGGAGAAGGATGA CCTCACAAAGGGACATCTCACGCTGTACCATAGCTACGTGTCAGGGGC |
| mm4_intron_Tshr_chr12_91512047 |
ACTGGGTAACTGCCACCATCCCCCACATCTCACTCCCAGGACTATCCAGAGGGTCTCAGTTCTGTTGGGATCTATTTTAA GTATCTCAGGCCTTGAAAATAATGATGAAGGAACTTGACAAGGGCCATCTGG |
| mm4_intergenic_Cyp3a59|Gm6309_chr5_146159220 |
AATCGCCCTACCCCACTGTACAGATAGGCCATTAACTCCCTCTTCCCCCAACCCCCGACTCCACAGATGGGCCCTCACTT CCCTATTTCCCCTACCCCATCTTACCA |
| mm4_intron_Gm6408_chr5_146483250 |
ACGGAGGTAAGAGCCCATCTGTAGAGTTGGAAGAAGATAAAGGTAAGGGTCAATTTGTGTGGTAGGGGAAATAGGGAAGT GAGGGCCCATCTGTGGAGTCAGGGGTTGGGGGAAGAGGGAGTTAATGGCC |
| mm4_intergenic_1810041L15Rik|Ldoc1l_chr15_84526582 |
GGCCAGGGGTGGACTATACTGAGCAAGTGCTAGTCAGCATCTAACTCTCATCTTCCTCTTGACCGTTTGTGTCCATCTGG GAGGTGCGGGGATGGGAAGAGGGGTCAAGGAACCTGCTTT |
| mm3_intron_Klf8_chrX_153293911 |
AGAAACTGGTGGTGAAAGAATCAGCTGTATATCCATCCATCCCCGACCTCTGCAGAAGGAAGAAGTTTTCTTCTAGCAGT TTCAATATTTTGAGTCTCA |
| mm4_intron_Tcf4_chr18_69613175 |
CGATCTAAATGAGGAGGAATCAGCCCATCTTGAGACTCGGGGGTTGGGGATTGCTCTCTGTCCCCCTTCCTACAGTTGCC AG |
| mm4_intergenic_Syngr2|Tk1_chr11_117815338 |
CCCCATGCCAGCTAGAAACACCATCTCCTGACTCTCTAGCAGGTTCTTTGCCATTTTTTGTCACGAAACCAACCAGATAG AAATGTGGGGGACTGTTCTTCTTGGGCTGTGGTGAGAC |
| mm4_exon_Gm13474_chr2_38976514 |
TCACTGTGCCTGCTTCCTTTCTCAGAGGAGCTGCCATCCCCAGAGCCTCCAGCTGGGCCCTCGGAGGCCGAAGAGTGGCT GCAGGGAGAGGCTGAGCAGCTGCAGAAGGAGCTGGAAAGTCTAATGGGACAGCTGCG |
| mm4_intron_Sil1_chr18_35384221 |
CCCAGCTCGCTCCTATTAGCTCATTTTCTAAGAGCAACTTCAGAGATAGTGAAGGAAAGAACCCTTCTGAAATCTACTTC CCATGGGTCCCAACCCCAGGCTCTCCAGCTGGGCTCCCAACTACACTGCAGCT |
| mm4_intron_Megf11_chr9_64389752 |
GGGACTCCCAGGACACTCTTATATGCGGTCCCATCCCCCCACTCCCCAGGTGGCCGTTGAATTAAAAGAATATTTTATCT TCATCAGCACACACACAGAGA |
| mm4_intron_Cxcl1_chr5_90892426 |
AGAACTCTGGGTGCAAGTGTAGACATGTAGAAAGCCCATCTGGAGCCTCAGGGGCTGGAATAAAAGAAGAAAGGCTTTGA TGACTATTTGTAACAATGACTCTTTTCTGATTGCAGAGGTGTCCCCAAGTAACGG |
| mm4_intron_Tesc_chr5_118039478 |
GCACGGGCTGATGTCTGTATACATGGCGAGTCCCCTGTCTGGAGAGTCCTGGCGGGGTCTGTGGGATGCTAAGGTCCTAC TTGGTTGAGCCAAGCATCCGTATTCAGGTTGTAGTGGCTGCTG |
| mm4_intron_Lhfp_chr3_53168683 |
GCGTGTACATCTGCTTTGTGATGTGACCTTCTGCAGAGGCTGGGAATGGATTAAAATATATAAGTAGGCATCCTCATCAT AGTCTGAAGTCTGGACAAGCAGACACAGTTCTCCACAAGGACCTCC |
| mm3_exon_Tnfaip2_chr12_111445096 |
CCGAGGCTGAATCAGAGACCTCCATGTCTGAGGCGTCCTCTGAGGACCTGATGCCATCCCCGGAGGCTCCCGATGGGGAG GAGGAGTCTGCGAAGAAGAAGGAGAAGAAGTCCAAAGGACTGGCCAACATGTTCA |
| mm4_intergenic_Gm16029/Egflam|Gm16029_chr15_7405104 |
TGTAGGCACACACATCACATAGTAATCACAGGACCAAAGCCATCCCCTGTCTCTGCAAAGGGTGGAGGAAGTCAAATCAC AATTTCCAGTCAGCCTATATCCAGTCTACCCACACAGCAG |
| mm4_intron_Hivep3_chr4_119993170 |
GGACTGCTCACTGTCGAGTCTGCCCTTCCCGTCCTTTACACTACCACTGCTCTGTGGTGTGTGGCTCAGAGCTTTTCCCT TCAGCATAGTCCTGGGATGGTAAATTTGGGGTGAGATGGATGCAAGCT |
| mm3_intergenic_Prkch|Hif1a_chr12_73815232 |
AGCTGACCTCCTGGCTCATAGAGAACTTCTCCACACCAAGATGGCAGAGACACTGGGCCTGCCTTCTCATGCTTTCTGCT GGAGAGTCCAGGGATTGAGGAAGAAGCAATGCCGAGTTGTCTGA |
| mm3_intron_Ezr_chr17_6758120 |
CTCTGCTGCTGTAGAGCCACCTGGCACTACCCTGCCCTGACTCTCCAGAAGAGACTGGGTCAAGCATGGGCTGTACCACG CGTAACCTCCGAAGGTGCTGGAATTCCCT |
| mm4_intron_Wscd2_chr5_113497178 |
AATGGGATGGGGCTTCTCAGGGCCATGTGGACAGTGCGGGGAGGGCAGTGGCTATGATGCAGGTGTTTGAGAGGCTATCT GTGTCCCTTCTAGCCTTGCCTG |