CRISPOR

DNA break repair outcome predictions

Guide: TTCAGGCAGCGGGCTGCTCA CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GGCACCATCTTCTCTGGCGGTTCAGGCAGCGGGCTGCTCACGGCTTCGGACAAATTAGAGACCCTCTTACCAACCAGAGT`	(Wild-type sequence)
424	`TCTCTGGCGGTTCAGGCAGCGGGCT----------TCGGACAAATTAGAGACCCTCTTAC`	`10 bp deleted → frameshift`
352	`TCTCTGGCGGTTCAGGCAGCGGGCTGCT-------TCGGACAAATTAGAGACCCTCTTAC`	`7 bp deleted → frameshift`
346	`TCTCTGGCGGTTCAGGCAGCGG-----------CTTCGGACAAATTAGAGACCCTCTTAC`	`11 bp deleted → frameshift`
256	`TCTCTGGCGGTTCAGGCAGCGG-----------------ACAAATTAGAGACCCTCTTAC`	`17 bp deleted → frameshift`
256	`TCTCTGGCGGTTCAGGC-----------------TTCGGACAAATTAGAGACCCTCTTAC`	`17 bp deleted → frameshift`
201	`TCTCTGGCGGTTCAGGCAGCGGGCTGCTC--------GGACAAATTAGAGACCCTCTTAC`	`8 bp deleted → frameshift`
198	`TCTCTGGCGGTTCAGGCAGC--------------TTCGGACAAATTAGAGACCCTCTTAC`	`14 bp deleted → frameshift`
190	`TCTCTGGCGG-----------------------CTTCGGACAAATTAGAGACCCTCTTAC`	`23 bp deleted → frameshift`
179	`TCTCTGGCGGTTCAGGCAGCGGG----------------ACAAATTAGAGACCCTCTTAC`	`16 bp deleted → frameshift`
164	`TCTCTGGCGGTTCAGGCAGCGGGCTGCTCA------------AATTAGAGACCCTCTTAC`	`12 bp deleted → no frameshift`
163	`TCTCTGGC--------------------------TTCGGACAAATTAGAGACCCTCTTAC`	`26 bp deleted → frameshift`
141	`TCTCTGGCGG-----------------------------ACAAATTAGAGACCCTCTTAC`	`29 bp deleted → frameshift`
129	`TCTCTGGCGGTTCAGGCAGCGGGCTGCTC---------------------------TTAC`	`27 bp deleted → no frameshift`
126	`TCTCTGGCGGTTCAGG-----------------------ACAAATTAGAGACCCTCTTAC`	`23 bp deleted → frameshift`
120	`TCTCTGGCGGTTC------------------------GGACAAATTAGAGACCCTCTTAC`	`24 bp deleted → no frameshift`
90	`TCTCTGGCGGTTCAGGCA------------------------AATTAGAGACCCTCTTAC`	`24 bp deleted → no frameshift`
80	`TCTCTGG--------------------------------ACAAATTAGAGACCCTCTTAC`	`32 bp deleted → frameshift`
74	`TCTCTGGCGGTTCA----------------------------AATTAGAGACCCTCTTAC`	`28 bp deleted → frameshift`
70	`TCTCTGGCGGTTCAGGCAGCGGGCTGCT-----------------------------TAC`	`29 bp deleted → frameshift`
70	`TCTCTGGCGGTTCAGGCAG-----------------------------AGACCCTCTTAC`	`29 bp deleted → frameshift`
66	`TCTCTGGCGGTTCAGGCAGCGGGCT------------------------------CTTAC`	`30 bp deleted → no frameshift`
66	`TCTCT------------------------------TCGGACAAATTAGAGACCCTCTTAC`	`30 bp deleted → no frameshift`
63	`TCTCTGGCGGTTCAGGCAG-------------------------------ACCCTCTTAC`	`31 bp deleted → frameshift`
60	`TCTCTGGCGGTTCAGGCAGCGGGCT--------------------------------TAC`	`32 bp deleted → frameshift`
60	`TCT--------------------------------TCGGACAAATTAGAGACCCTCTTAC`	`32 bp deleted → frameshift`
57	`TCTCTGGCGGTTCAG---------------------------------AGACCCTCTTAC`	`33 bp deleted → no frameshift`
57	`TCTC---------------------------------GGACAAATTAGAGACCCTCTTAC`	`33 bp deleted → no frameshift`
52	`TCTCTGGCGGTTCAG-----------------------------------ACCCTCTTAC`	`35 bp deleted → frameshift`
52	`TC-----------------------------------GGACAAATTAGAGACCCTCTTAC`	`35 bp deleted → frameshift`
44	`TCTCT----------------------------------------------------TAC`	`52 bp deleted → frameshift`
36	`TCTCTGGCGGTT----------------------------------AGAGACCCTCTTAC`	`34 bp deleted → frameshift`
34	`TCTCTGGCGGTTC-------------------------------------------TTAC`	`43 bp deleted → frameshift`
26	`TCT------------------------------------------------------TAC`	`54 bp deleted → no frameshift`
24	`TCTCT--------------------------------------------------CTTAC`	`50 bp deleted → frameshift`
20	`TCTCTGGCGGTT----------------------------------------------AC`	`46 bp deleted → frameshift`

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