CRISPOR

DNA break repair outcome predictions

Guide: AGAGGAGCGCTCGCATCACA AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TCCCAGAGGCGGTGCGGCACAGAGGAGCGCTCGCATCACAAGGTGACCCTAGCTCCCCACTGCCATCTCCGCGGTCGCCG`	(Wild-type sequence)
221	`GGTGCGGCACAGAGGAGCGCTC-----------------------CCCACTGCCATCTCC`	`23 bp deleted → frameshift`
203	`GGTGCGGCACAGAGG------------------TGACCCTAGCTCCCCACTGCCATCTCC`	`18 bp deleted → no frameshift`
201	`GGTGCGGCACAGAGGAGCGCTCGCATCAC--------CCTAGCTCCCCACTGCCATCTCC`	`8 bp deleted → frameshift`
175	`GGTGCGGCACAGAGGAGCGCTCGCATCAC---------------------TGCCATCTCC`	`21 bp deleted → no frameshift`
154	`GGTGCGGCACAGAGGAGCGCTCGC-------------------TCCCCACTGCCATCTCC`	`19 bp deleted → frameshift`
148	`GGTG-------------------------------ACCCTAGCTCCCCACTGCCATCTCC`	`31 bp deleted → frameshift`
143	`GGTGCGGCACAGAGGAGC-------------------------TCCCCACTGCCATCTCC`	`25 bp deleted → frameshift`
141	`GGTGCGGCACAGAGGAG---------------GTGACCCTAGCTCCCCACTGCCATCTCC`	`15 bp deleted → no frameshift`
133	`GGTGCGGCACAGAGGAGCGCTCGCATC------------------------------TCC`	`30 bp deleted → no frameshift`
122	`GGTGCGGCACAGAGGAGCGCTCGCATC------------------CCCACTGCCATCTCC`	`18 bp deleted → no frameshift`
116	`GGTGCGGCACAGAGGAGCGCTCGCATCACA-------------------CTGCCATCTCC`	`19 bp deleted → frameshift`
116	`GGTGCGGCACAGAGGAGCGCT-------------------AGCTCCCCACTGCCATCTCC`	`19 bp deleted → frameshift`
110	`GGTGCGGCACAGAGGA--------------------CCCTAGCTCCCCACTGCCATCTCC`	`20 bp deleted → frameshift`
110	`GGTGCGGCACAG--------------------GTGACCCTAGCTCCCCACTGCCATCTCC`	`20 bp deleted → frameshift`
109	`GGTGCGG--------------------------TGACCCTAGCTCCCCACTGCCATCTCC`	`26 bp deleted → frameshift`
95	`GGTGCGGCACAGA-----------------------CCCTAGCTCCCCACTGCCATCTCC`	`23 bp deleted → frameshift`
94	`GGTGCGGCACAGAGGAGCGCTCGC-----------------------------CATCTCC`	`29 bp deleted → frameshift`
90	`GGTGCGGCACAGAGGAGCGCTCGCA------------------------CTGCCATCTCC`	`24 bp deleted → no frameshift`
86	`GGTGCGGCACAGAGGAGCGCTCGCATCACA-------------------------TCTCC`	`25 bp deleted → frameshift`
78	`GGTGCGGCACAGAGGAGCGCTC-------------------------------------C`	`37 bp deleted → frameshift`
77	`GGTGCGGCACAGAGGAGCGCTCGCATCA---------------------------TCTCC`	`27 bp deleted → no frameshift`
77	`GGTGCGGCAC---------------------------CCTAGCTCCCCACTGCCATCTCC`	`27 bp deleted → no frameshift`
76	`GGTGCGGCACAGAGGAGCGC---------------------------------CATCTCC`	`33 bp deleted → no frameshift`
74	`GGTGCGGCACAGAG----------------------------CTCCCCACTGCCATCTCC`	`28 bp deleted → frameshift`
69	`GGTGCGGCACAGAGGAGC-----------------------------------CATCTCC`	`35 bp deleted → frameshift`
69	`GGTGCGGC-----------------------------------TCCCCACTGCCATCTCC`	`35 bp deleted → frameshift`
67	`GGTGCGGCAC----------------------------------------TGCCATCTCC`	`40 bp deleted → frameshift`
66	`GGTGCGGCACAGAGGAGCGCT------------------------------GCCATCTCC`	`30 bp deleted → no frameshift`
66	`GGTGCGGCACAG------------------------------CTCCCCACTGCCATCTCC`	`30 bp deleted → no frameshift`
60	`GGTGCGGCACAGAGGAGCGCTCGCATC--------------------------------C`	`32 bp deleted → frameshift`
60	`GGTGC--------------------------------------TCCCCACTGCCATCTCC`	`38 bp deleted → frameshift`
52	`GGTGCGGCACAGAGGAGCGCTC-----------------------------------TCC`	`35 bp deleted → frameshift`
45	`GGTGC------------------------------------------------CATCTCC`	`48 bp deleted → no frameshift`
45	`GGTGCGGCACA--------------------------------------CTGCCATCTCC`	`38 bp deleted → frameshift`
42	`GGTGCGGC---------------------------------------------CATCTCC`	`45 bp deleted → no frameshift`
33	`GGTGCGGCACA--------------------------------------------TCTCC`	`44 bp deleted → frameshift`
30	`GGTGCGGCA----------------------------------------------TCTCC`	`46 bp deleted → frameshift`

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