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The following table lists possible deletions.
Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.
| mh- Score | Sequence | Effect |
|---|---|---|
| TCGCCTTAGCTCCTCTTCTCAGCCAAGATCCCAGGGAGCCTGGGATTAGGAGCTTACTTGGGGGTTTTCCCCCTCCCCCC | (Wild-type sequence) | |
| 469 | TCCTCTTCTCAGCCAAGATCCCAGGGA--------TTAGGAGCTTACTTGGGGGTTTTCC | 8 bp deleted → frameshift |
| 447 | TCCTCTTCTCAGCCAAGATCCCAGGGAGC--------------TTACTTGGGGGTTTTCC | 14 bp deleted → frameshift |
| 282 | TCCTCTTCTCAGCCAAGATCCCAGGG-------GATTAGGAGCTTACTTGGGGGTTTTCC | 7 bp deleted → frameshift |
| 255 | TCCTCTTCTCAGCCAAGATCCCAGG---------ATTAGGAGCTTACTTGGGGGTTTTCC | 9 bp deleted → no frameshift |
| 235 | TCCTCTTCTCAGCCAAGATCCCAGG---------------AGCTTACTTGGGGGTTTTCC | 15 bp deleted → no frameshift |
| 173 | TCCTCTTCTCAGCCAAGATCCCAGGGAG-----------GAGCTTACTTGGGGGTTTTCC | 11 bp deleted → frameshift |
| 170 | TCCTCTTCTCAGCCAAGAT-----------------TAGGAGCTTACTTGGGGGTTTTCC | 17 bp deleted → frameshift |
| 163 | TCCTCTTCTCAGCCAAGATCCCAGGG--------------------------GGTTTTCC | 26 bp deleted → frameshift |
| 155 | TCCTCTTCTCAGCCAAGATCCCAGGG---------------------------GTTTTCC | 27 bp deleted → no frameshift |
| 148 | TCCTCTTCTCAGCCAAGATCCCAGGG----------------------------TTTTCC | 28 bp deleted → frameshift |
| 122 | TCCTCTTCTCAGCCAAGATCCCAG------------------CTTACTTGGGGGTTTTCC | 18 bp deleted → no frameshift |
| 114 | TCCTCTTCTCAGCCAAGATCCCAGGG-------------------------GGGTTTTCC | 25 bp deleted → frameshift |
| 111 | TCCTCTTCTCAGC------------------------------TTACTTGGGGGTTTTCC | 30 bp deleted → no frameshift |
| 99 | TCCTCTTCTCAGCCAAG----------------------GAGCTTACTTGGGGGTTTTCC | 22 bp deleted → frameshift |
| 95 | TCCTCTTCTCAGCCAAGA-----------------------GCTTACTTGGGGGTTTTCC | 23 bp deleted → frameshift |
| 94 | TCCTCTTCTCAGCCAAGATCCCAGG-----------------------------TTTTCC | 29 bp deleted → frameshift |
| 89 | TCCTCTTCTCAGCCAAGATCCCAGGGAGCC------------------------------ | 30 bp deleted → no frameshift |
| 86 | TCCTCTTCTCAGCCAAG-------------------------CTTACTTGGGGGTTTTCC | 25 bp deleted → frameshift |
| 77 | TCCTCTTCTCAG---------------------------GAGCTTACTTGGGGGTTTTCC | 27 bp deleted → no frameshift |
| 71 | TCCTCTTCTCAGCCAAGATCC--------------------------------------- | 39 bp deleted → no frameshift |
| 60 | TCCTCTTCTCAGCCAAGATCCC-------------------------------------- | 38 bp deleted → frameshift |
| 60 | TCCTCTT--------------------------------------ACTTGGGGGTTTTCC | 38 bp deleted → frameshift |
| 52 | TCCTCTTCT-----------------------------------TACTTGGGGGTTTTCC | 35 bp deleted → frameshift |
| 48 | TCCTCTT------------------------------------------GGGGGTTTTCC | 42 bp deleted → no frameshift |
| 44 | TCCTCTT------------------------------AGGAGCTTACTTGGGGGTTTTCC | 30 bp deleted → no frameshift |
| 42 | TCCTCTTCT---------------------------------------TGGGGGTTTTCC | 39 bp deleted → no frameshift |
| 40 | TCCT----------------------------------------TACTTGGGGGTTTTCC | 40 bp deleted → frameshift |
| 40 | TCCTCTTCTCAGCC---------------------------------------------- | 46 bp deleted → frameshift |
| 33 | TCCT--------------------------------------------TGGGGGTTTTCC | 44 bp deleted → frameshift |
| 31 | TCCTCTTC---------------------------------------------------C | 51 bp deleted → no frameshift |
| 29 | TCC--------------------------------------------------------- | 57 bp deleted → no frameshift |
| 25 | TCCTCTTCTC-------------------------------------------------C | 49 bp deleted → frameshift |
| 20 | TCCTC------------------------------------------------------C | 54 bp deleted → no frameshift |
| 17 | TCCTCTT-------------------------------------------------TTCC | 49 bp deleted → frameshift |
| 16 | TCCTCTT--------------------------------------------------TCC | 50 bp deleted → frameshift |