CRISPOR

DNA break repair outcome predictions

Guide: CTCCCGGGAAGTCGGAGGGC CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TCCTGGCCTCCCCCGCCACGCTCCCGGGAAGTCGGAGGGCCGGCTCGCGGGCTCAGGCAACCGTGGGGCACCATCTTCTC`	(Wild-type sequence)
491	`CCCCGCCACGCTCCCGGGAAGTCGGAGGGC----TCGCGGGCTCAGGCAACCGTGGGGCA`	`4 bp deleted → frameshift`
439	`CCCCGCCACGCTCCCGGGAAGTCGGAGGGC------------TCAGGCAACCGTGGGGCA`	`12 bp deleted → no frameshift`
402	`CCCCGCCACGCTCCCGGGAAGTCGG--------CTCGCGGGCTCAGGCAACCGTGGGGCA`	`8 bp deleted → frameshift`
311	`CCCCGCCACGCTCCCGGGAAGTCGGAGG-----CTCGCGGGCTCAGGCAACCGTGGGGCA`	`5 bp deleted → frameshift`
283	`CCCCGCCACGCTCCCGGGAAGTCGG---------------GCTCAGGCAACCGTGGGGCA`	`15 bp deleted → no frameshift`
269	`CCCCGCCACGCTCCCGG----------------CTCGCGGGCTCAGGCAACCGTGGGGCA`	`16 bp deleted → frameshift`
268	`CCCCGCCACGCTCCCGGGAAGTCGGAGGGC--------GGGCTCAGGCAACCGTGGGGCA`	`8 bp deleted → frameshift`
261	`CCCCGCCACGCTCCCGGGAAGTCG-------------CGGGCTCAGGCAACCGTGGGGCA`	`13 bp deleted → frameshift`
253	`CCCCGCCACGCTCCCGGG-----------------------CTCAGGCAACCGTGGGGCA`	`23 bp deleted → frameshift`
244	`CCCCGCCACGCTCCCGGGAAGTCGGAGGGC------------------AACCGTGGGGCA`	`18 bp deleted → no frameshift`
230	`CCCCGCCACGCTCCCGGGAAGTCGGAGGG-----------GCTCAGGCAACCGTGGGGCA`	`11 bp deleted → frameshift`
221	`CCCCGCCACGCTC-----------------------GCGGGCTCAGGCAACCGTGGGGCA`	`23 bp deleted → frameshift`
208	`CCCCGCCACGCTCCCGGGAAGTCGGAGG-------------CTCAGGCAACCGTGGGGCA`	`13 bp deleted → frameshift`
193	`CCCCGCCACGCTCCCGGGAAGTCGGAGG-------------------CAACCGTGGGGCA`	`19 bp deleted → frameshift`
188	`CCCCGCCACGCTCCCGGG---------------CTCGCGGGCTCAGGCAACCGTGGGGCA`	`15 bp deleted → no frameshift`
188	`CCCCGCCACGCTCCCGGGAAGTCGGAGGGC-----------------------------A`	`29 bp deleted → frameshift`
179	`CCCCGCCACGCTCCCGGGAAGTCGG----------------CTCAGGCAACCGTGGGGCA`	`16 bp deleted → frameshift`
155	`CCCCGCCACGC---------------------------GGGCTCAGGCAACCGTGGGGCA`	`27 bp deleted → no frameshift`
148	`CCCCGCCACGCTC-------------------------------AGGCAACCGTGGGGCA`	`31 bp deleted → frameshift`
148	`CCCCGCCACGCTCCCGGGAAGTCGGAGGG----------------------------GCA`	`28 bp deleted → frameshift`
140	`CCCCGCCACGCTCCCG---------------------CGGGCTCAGGCAACCGTGGGGCA`	`21 bp deleted → no frameshift`
133	`CCCCGCCACGCTCCCGGGAAGTCGG----------------------CAACCGTGGGGCA`	`22 bp deleted → frameshift`
133	`CCCCGCCACGCTCCCGGG----------------------GCTCAGGCAACCGTGGGGCA`	`22 bp deleted → frameshift`
133	`CCCCGCCACG----------------------GCTCGCGGGCTCAGGCAACCGTGGGGCA`	`22 bp deleted → frameshift`
121	`CCCCGC--------------------------------GGGCTCAGGCAACCGTGGGGCA`	`32 bp deleted → frameshift`
120	`CCCCGCCACGCTCCCGG------------------------CTCAGGCAACCGTGGGGCA`	`24 bp deleted → no frameshift`
105	`CCCCGCCACGCTCCCGGGAAGTC---------------------AGGCAACCGTGGGGCA`	`21 bp deleted → no frameshift`
103	`CCCCGCCACGCTCCCGGGAAGTCGGAGGG---------------------------GGCA`	`27 bp deleted → no frameshift`
103	`CCCCG---------------------------GCTCGCGGGCTCAGGCAACCGTGGGGCA`	`27 bp deleted → no frameshift`
98	`CCCCGC----------------------------TCGCGGGCTCAGGCAACCGTGGGGCA`	`28 bp deleted → frameshift`
94	`CCCCGCCACGCTCCCG-------------------------------------TGGGGCA`	`37 bp deleted → frameshift`
94	`CCCCGCCACGCTCCCGGGAAGTCG-----------------------------TGGGGCA`	`29 bp deleted → frameshift`
94	`CCCCGCCACGCTCCCGGG-----------------------------CAACCGTGGGGCA`	`29 bp deleted → frameshift`
94	`CCCCGCCACG-----------------------------GGCTCAGGCAACCGTGGGGCA`	`29 bp deleted → frameshift`
89	`CCCCGCCACGCTCCCGGGAAGTCGGAGG------------------------------CA`	`30 bp deleted → no frameshift`
89	`CCCCGCCACGCTCCCGG------------------------------CAACCGTGGGGCA`	`30 bp deleted → no frameshift`
86	`CCCCGCCACGCTCCCGGGAAG-------------------------GCAACCGTGGGGCA`	`25 bp deleted → frameshift`
85	`CCCCGCCACGCTCCCGGG---------------------------------------GCA`	`39 bp deleted → no frameshift`
84	`CCCCGCCACGCTCCCGGGAAGTCGG-------------------------------GGCA`	`31 bp deleted → frameshift`
81	`CCCCGCCACGCTCCCGGG----------------------------------------CA`	`40 bp deleted → frameshift`
80	`CCCCGCCACGCTCCCGGGAAGTCGG--------------------------------GCA`	`32 bp deleted → frameshift`
76	`CCCCGCCACGCTCCCGGGAAGTCGG---------------------------------CA`	`33 bp deleted → no frameshift`
73	`CCCCG----------------------------------GGCTCAGGCAACCGTGGGGCA`	`34 bp deleted → frameshift`
66	`CCCCGC------------------------------------TCAGGCAACCGTGGGGCA`	`36 bp deleted → no frameshift`
62	`CCCCGCCACGC-------------------------------------AACCGTGGGGCA`	`37 bp deleted → frameshift`
60	`CCCCGCCACGCTCCCGGGAAGT--------------------------------GGGGCA`	`32 bp deleted → frameshift`
60	`CCCCGCCACGCTCCCGGG--------------------------------------GGCA`	`38 bp deleted → frameshift`
60	`CCCCGCCACGCTCC--------------------------------------GTGGGGCA`	`38 bp deleted → frameshift`
54	`CCCCG------------------------------------------------TGGGGCA`	`48 bp deleted → no frameshift`
51	`CCCCGCCACGCTCCCGG-----------------------------------------CA`	`41 bp deleted → frameshift`
48	`CCCCGC------------------------------------------AACCGTGGGGCA`	`42 bp deleted → no frameshift`
47	`CCCCGCCA-------------------------------------GGCAACCGTGGGGCA`	`37 bp deleted → frameshift`
46	`CCCCGCCACG-------------------------------------------TGGGGCA`	`43 bp deleted → frameshift`
44	`CCCCGCCACGCTCCCGGGAA------------------------------CCGTGGGGCA`	`30 bp deleted → no frameshift`
42	`CCCCGCC---------------------------------------------GTGGGGCA`	`45 bp deleted → no frameshift`
38	`CCCCGCCA-----------------------------------------ACCGTGGGGCA`	`41 bp deleted → frameshift`
36	`CCCCGCCAC------------------------------------------CGTGGGGCA`	`42 bp deleted → no frameshift`
36	`CCCCGCCACGC------------------------------------------------A`	`48 bp deleted → no frameshift`
34	`CCC-------------------------------------------------GTGGGGCA`	`49 bp deleted → frameshift`
32	`CC--------------------------------------------------GTGGGGCA`	`50 bp deleted → frameshift`
28	`CCCCGC-----------------------------------------------------A`	`53 bp deleted → frameshift`
22	`CCCCGCCA----------------------------------------------------`	`52 bp deleted → frameshift`

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