CRISPOR

DNA break repair outcome predictions

Guide: CCTCCGACTTCCCGGGAGCG TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GCCTGAGCCCGCGAGCCGGCCCTCCGACTTCCCGGGAGCGTGGCGGGGGAGGCCAGGAGGAGGAGCCGGTCGATGGACTA`	(Wild-type sequence)
469	`GCGAGCCGGCCCTCCGACTTCCCGGGAG-------------GCCAGGAGGAGGAGCCGGT`	`13 bp deleted → frameshift`
467	`GCGAGCCGGCCCTCCGACTTCCCGGGAGCG-----GGGGAGGCCAGGAGGAGGAGCCGGT`	`5 bp deleted → frameshift`
461	`GCGAGCCGGCCCTCCGACTTCCCGGG-----------GGAGGCCAGGAGGAGGAGCCGGT`	`11 bp deleted → frameshift`
329	`GCGAGCCGGCCCTCCGACTTCCCGGG------------GAGGCCAGGAGGAGGAGCCGGT`	`12 bp deleted → no frameshift`
282	`GCGAGCCGGCCCTCCGACTTCCCGGG-------CGGGGGAGGCCAGGAGGAGGAGCCGGT`	`7 bp deleted → frameshift`
268	`GCGAGCCGGCCCTCCGACTTCCCGG--------CGGGGGAGGCCAGGAGGAGGAGCCGGT`	`8 bp deleted → frameshift`
245	`GCGAGCCGGCCCTCCGACTTCCCGGGAG---------------------GAGGAGCCGGT`	`21 bp deleted → no frameshift`
242	`GCGAGCCGGCCCTCCGACTTCCCGGG----------GGGAGGCCAGGAGGAGGAGCCGGT`	`10 bp deleted → frameshift`
233	`GCGAGCCGGCCCTCCGACTTCCCGGGAGC---------------------------CGGT`	`27 bp deleted → no frameshift`
210	`GCGAGCCGGCCCTCCGACTTCCCGGGAG------------------------GAGCCGGT`	`24 bp deleted → no frameshift`
198	`GCGAGCCGGCCCTCCGACTTCCCGGGAGC--------------CAGGAGGAGGAGCCGGT`	`14 bp deleted → frameshift`
198	`GCGAGCCGGCCCTCCGACTTCCCGG--------------AGGCCAGGAGGAGGAGCCGGT`	`14 bp deleted → frameshift`
180	`GCGAGCCGGC------------------------GGGGGAGGCCAGGAGGAGGAGCCGGT`	`24 bp deleted → no frameshift`
179	`GCGAGCCGGCCCTCCGACTTCCCGGG----------------CCAGGAGGAGGAGCCGGT`	`16 bp deleted → frameshift`
170	`GCGAGCCGGCCCTCCGACTTCCCGG-----------------CCAGGAGGAGGAGCCGGT`	`17 bp deleted → frameshift`
155	`GCGAGCCGG---------------------------GGGAGGCCAGGAGGAGGAGCCGGT`	`27 bp deleted → no frameshift`
154	`GCGAGCCGGCCCTCCG-------------------GGGGAGGCCAGGAGGAGGAGCCGGT`	`19 bp deleted → frameshift`
153	`GCGAGCCGGCC---------------------------------AGGAGGAGGAGCCGGT`	`33 bp deleted → no frameshift`
146	`GCGAGCCGGCCCTCCGACTTCCCGG----------------------------------T`	`34 bp deleted → frameshift`
140	`GCGAGCCGGCCCTCCGACTTCCC---------------------AGGAGGAGGAGCCGGT`	`21 bp deleted → no frameshift`
133	`GCGAGCCGGCCCTCCGACTTCCCGG----------------------AGGAGGAGCCGGT`	`22 bp deleted → frameshift`
133	`GCGAGCCGGCCCTCCGACTTCC----------------------AGGAGGAGGAGCCGGT`	`22 bp deleted → frameshift`
122	`GCGAGCCGGCCCTCCGACTTCCCGGGAG------------------GAGGAGGAGCCGGT`	`18 bp deleted → no frameshift`
121	`GCG--------------------------------GGGGAGGCCAGGAGGAGGAGCCGGT`	`32 bp deleted → frameshift`
114	`GCGAGCCGGCCCTCCGACTTCCCGG-------------------------AGGAGCCGGT`	`25 bp deleted → frameshift`
106	`GCGAGCCGG--------------------------------------------------T`	`50 bp deleted → frameshift`
98	`GCGAGCCGGCCCTCCGACTTCCCGGGAGCG----------------------------GT`	`28 bp deleted → frameshift`
98	`GCGAGCCGGCCCTCCGACTTCCCGG----------------------------AGCCGGT`	`28 bp deleted → frameshift`
98	`GCGAGCCGG----------------------------GGAGGCCAGGAGGAGGAGCCGGT`	`28 bp deleted → frameshift`
98	`GCGAGC----------------------------GGGGGAGGCCAGGAGGAGGAGCCGGT`	`28 bp deleted → frameshift`
95	`GCGAGCCGGCCCTCCGA-----------------------GGCCAGGAGGAGGAGCCGGT`	`23 bp deleted → frameshift`
94	`GCGAGCC-------------------------------------AGGAGGAGGAGCCGGT`	`37 bp deleted → frameshift`
94	`GCGAGCCGGCCCTCC-----------------------------AGGAGGAGGAGCCGGT`	`29 bp deleted → frameshift`
94	`GCGAGCCGG-----------------------------GAGGCCAGGAGGAGGAGCCGGT`	`29 bp deleted → frameshift`
89	`GCGAGCCGG------------------------------AGGCCAGGAGGAGGAGCCGGT`	`30 bp deleted → no frameshift`
82	`GCGAG------------------------------------GCCAGGAGGAGGAGCCGGT`	`36 bp deleted → no frameshift`
80	`GCGAGCCGGCCC--------------------------------AGGAGGAGGAGCCGGT`	`32 bp deleted → frameshift`
76	`GCGAGCCGGCCCTCCGACTTCCCGGG---------------------------------T`	`33 bp deleted → no frameshift`
73	`GCGAGCCGGCCCTCCG------------------------------------------GT`	`42 bp deleted → no frameshift`
69	`GCGAGCCGGCCCTCCGACTTCC-----------------------------------GGT`	`35 bp deleted → frameshift`
63	`GCGAGCCGGCCCTCCGA-------------------------------GGAGGAGCCGGT`	`31 bp deleted → frameshift`
60	`GCGAGCCGGCC----------------------------------------------GGT`	`46 bp deleted → frameshift`
60	`GCGAGCCGG--------------------------------------AGGAGGAGCCGGT`	`38 bp deleted → frameshift`
55	`GCGAG--------------------------------------------GAGGAGCCGGT`	`44 bp deleted → frameshift`
54	`GCGAGCCGGCCCTCCGA----------------------------------GGAGCCGGT`	`34 bp deleted → frameshift`
51	`GCGAGCCGG-----------------------------------------AGGAGCCGGT`	`41 bp deleted → frameshift`
51	`GC-----------------------------------------CAGGAGGAGGAGCCGGT`	`41 bp deleted → frameshift`
47	`GCGAG-----------------------------------------------GAGCCGGT`	`47 bp deleted → frameshift`
47	`GCGAGCCGGCCCTCCGA-------------------------------------GCCGGT`	`37 bp deleted → frameshift`
44	`GCGAGCCGG--------------------------------------------AGCCGGT`	`44 bp deleted → frameshift`
42	`GCGAGCCGGCCC---------------------------------------------GGT`	`45 bp deleted → no frameshift`
38	`GCGAG-----------------------------------------GAGGAGGAGCCGGT`	`41 bp deleted → frameshift`
26	`GC------------------------------------------------------CGGT`	`54 bp deleted → no frameshift`
25	`GCG-------------------------------------------------------GT`	`55 bp deleted → frameshift`

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