CRISPOR

DNA break repair outcome predictions

Guide: GCGACCGCGGAGATGGCAGT GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GCCCCAGCGCCGTGTCGACGGCGACCGCGGAGATGGCAGTGGGGAGCTAGGGTCACCTTGTGATGCGAGCGCTCCTCTGT`	(Wild-type sequence)
347	`CGTGTCGACGGCGACCGCGGAG--------------CTAGGGTCACCTTGTGATGCGAGC`	`14 bp deleted → frameshift`
296	`CGTGTCGACGGCGACCGCGGAGATGG------GGAGCTAGGGTCACCTTGTGATGCGAGC`	`6 bp deleted → no frameshift`
282	`CGTGTCGACGGCGACCGCGGAGATGG-------GAGCTAGGGTCACCTTGTGATGCGAGC`	`7 bp deleted → frameshift`
268	`CGTGTCGACGGCGACCGCGGAGATGG--------AGCTAGGGTCACCTTGTGATGCGAGC`	`8 bp deleted → frameshift`
242	`CGTGTCGACGGCGACCGCGGAGATGGC----------TAGGGTCACCTTGTGATGCGAGC`	`10 bp deleted → frameshift`
219	`CGTGTCGACGGCGACCGCGG------------GGAGCTAGGGTCACCTTGTGATGCGAGC`	`12 bp deleted → no frameshift`
211	`CGTGTCGACGGCGACCGCGGAGATGGCAG-------CTAGGGTCACCTTGTGATGCGAGC`	`7 bp deleted → frameshift`
208	`CGTGTCGACGGCGACCGCGG-------------GAGCTAGGGTCACCTTGTGATGCGAGC`	`13 bp deleted → frameshift`
188	`CGTGTCGACGGCGACCGCGGAGATGG---------------GTCACCTTGTGATGCGAGC`	`15 bp deleted → no frameshift`
179	`CGTGTCGACGGCGACCGCGGAGATGG----------------TCACCTTGTGATGCGAGC`	`16 bp deleted → frameshift`
173	`CGTGTCGACGGCGACCGCGGAGATGGCAG-----------GGTCACCTTGTGATGCGAGC`	`11 bp deleted → frameshift`
164	`CGTGTCGACGGCGACCGCGGAGA------------GCTAGGGTCACCTTGTGATGCGAGC`	`12 bp deleted → no frameshift`
156	`CGTGTCGACGGCGACCGCGGAGATGGCAGT-------------CACCTTGTGATGCGAGC`	`13 bp deleted → frameshift`
154	`CGTGTCGACGGCGACCGC-------------------TAGGGTCACCTTGTGATGCGAGC`	`19 bp deleted → frameshift`
140	`CGTGTCGACGGCGACCGCGG---------------------GTCACCTTGTGATGCGAGC`	`21 bp deleted → no frameshift`
140	`CGTGTCGACGG---------------------GGAGCTAGGGTCACCTTGTGATGCGAGC`	`21 bp deleted → no frameshift`
133	`CGTGTCGACGGCGACCGCGGAGATG------------------------------CGAGC`	`30 bp deleted → no frameshift`
133	`CGTGTCGACGGCGACCGCGG----------------------TCACCTTGTGATGCGAGC`	`22 bp deleted → frameshift`
133	`CGTGTCGACGG----------------------GAGCTAGGGTCACCTTGTGATGCGAGC`	`22 bp deleted → frameshift`
128	`CGTGTCGACGGCGACCGCGGAGATGGCA-----------------CCTTGTGATGCGAGC`	`17 bp deleted → frameshift`
126	`CGTGTCGACGG-----------------------AGCTAGGGTCACCTTGTGATGCGAGC`	`23 bp deleted → frameshift`
122	`CGTGTCGACGGCGACCGCGGAG------------------GGTCACCTTGTGATGCGAGC`	`18 bp deleted → no frameshift`
114	`CGTGTCGACGGC-------------------------TAGGGTCACCTTGTGATGCGAGC`	`25 bp deleted → frameshift`
106	`CGTGTCGACGGCGACC-------------------------------TTGTGATGCGAGC`	`31 bp deleted → frameshift`
105	`CGTGTCGACGGCGACCGCGGAGATGGCAGT---------------------GATGCGAGC`	`21 bp deleted → no frameshift`
105	`CGTGTCGACGGCGA---------------------GCTAGGGTCACCTTGTGATGCGAGC`	`21 bp deleted → no frameshift`
94	`CGTGTCGACGGCGACCGCGGAGATGGC-----------------------------GAGC`	`29 bp deleted → frameshift`
90	`CGTGTCGACGGCGACCGCG--------------------------------------AGC`	`38 bp deleted → frameshift`
89	`CGTGTCGACGG------------------------------GTCACCTTGTGATGCGAGC`	`30 bp deleted → no frameshift`
86	`CGTGTCGACGGCGACCGCGGAGATG-------------------------TGATGCGAGC`	`25 bp deleted → frameshift`
84	`CGTGTCGACGG-------------------------------TCACCTTGTGATGCGAGC`	`31 bp deleted → frameshift`
78	`CGTGTCGACGGCGACCGCGGAG-------------------------------------C`	`37 bp deleted → frameshift`
77	`CGTGTCGACGGCGACCGCGGAGATG---------------------------ATGCGAGC`	`27 bp deleted → no frameshift`
77	`CGTGTCGACGGCGA--------------------------------------------GC`	`44 bp deleted → frameshift`
77	`CGTGTCGA---------------------------GCTAGGGTCACCTTGTGATGCGAGC`	`27 bp deleted → no frameshift`
76	`CGTGTCGACGGCGACCGCGGAGATGGC---------------------------------`	`33 bp deleted → no frameshift`
75	`CGTGTC--------------------------------------ACCTTGTGATGCGAGC`	`38 bp deleted → frameshift`
66	`CGTGTCGACGGCGACCGCGGAGATGGCAG------------------------------C`	`30 bp deleted → no frameshift`
60	`CGTGTCGACGGCGACCGCGGA--------------------------------TGCGAGC`	`32 bp deleted → frameshift`
54	`CGTGTCGACGGCGACCG----------------------------------------AGC`	`40 bp deleted → frameshift`
52	`CGTGTCGACGGCGACCGCGGAGA-----------------------------------GC`	`35 bp deleted → frameshift`
48	`CGTGTCGACGGCGACCGC------------------------------------------`	`42 bp deleted → no frameshift`
47	`CGTGTCGAC-------------------------------------CTTGTGATGCGAGC`	`37 bp deleted → frameshift`
45	`CGTG------------------------------------------------ATGCGAGC`	`48 bp deleted → no frameshift`
42	`CGTGTCGACGGCGA---------------------------------------TGCGAGC`	`39 bp deleted → no frameshift`
41	`CGTGTCGA--------------------------------------------------GC`	`50 bp deleted → frameshift`
40	`CGT----------------------------------------CACCTTGTGATGCGAGC`	`40 bp deleted → frameshift`
40	`CGTGT----------------------------------------------GATGCGAGC`	`46 bp deleted → frameshift`
38	`CGTGTCGACG-----------------------------------------------AGC`	`47 bp deleted → frameshift`
36	`CGTGTCGACGGC------------------------------------------------`	`48 bp deleted → no frameshift`
31	`CGTGTCGA---------------------------------------------TGCGAGC`	`45 bp deleted → no frameshift`
25	`CG-------------------------------------------------------AGC`	`55 bp deleted → frameshift`
23	`CGTG---------------------------------------------------CGAGC`	`51 bp deleted → no frameshift`

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