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The following table lists possible deletions.
Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.
| mh- Score | Sequence | Effect |
|---|---|---|
| GCCAAGCTGACCCCCATCCAGAAACATAACCATGTCCTTTCGGCTCTCCATCCCCGGACTCTCCAGAGGGGGGGAGGGGG | (Wild-type sequence) | |
| 274 | CCCCCATCCAGAAACATAACCATGTCC------------ATCCCCGGACTCTCCAGAGGG | 12 bp deleted → no frameshift |
| 244 | CCCCCATCCAGAAACATAACCAT------------------CCCCGGACTCTCCAGAGGG | 18 bp deleted → no frameshift |
| 224 | CCCCCATCCAGAAACATAACCATGTCC----------------CCGGACTCTCCAGAGGG | 16 bp deleted → frameshift |
| 211 | CCCCCATCCAGAAACATAACCATGTCCT-------CTCCATCCCCGGACTCTCCAGAGGG | 7 bp deleted → frameshift |
| 191 | CCCCCATCCAGAAACATAACCATGTCCT---------CCATCCCCGGACTCTCCAGAGGG | 9 bp deleted → no frameshift |
| 183 | CCCCCATCC----------------------------------CCGGACTCTCCAGAGGG | 34 bp deleted → frameshift |
| 182 | CCCCCATCCAGAAACATAACCATGTC----------TCCATCCCCGGACTCTCCAGAGGG | 10 bp deleted → frameshift |
| 170 | CCCCCATCCAGAAACATAACCATGTCC-----------------CGGACTCTCCAGAGGG | 17 bp deleted → frameshift |
| 162 | CCCCCATCCAGAAACATAACCATGTCC------------------GGACTCTCCAGAGGG | 18 bp deleted → no frameshift |
| 133 | CCCCCATCCA------------------------------TCCCCGGACTCTCCAGAGGG | 30 bp deleted → no frameshift |
| 133 | CCCCCATCCAGAAACATAACC----------------------CCGGACTCTCCAGAGGG | 22 bp deleted → frameshift |
| 129 | CCCCCATCCAGAAACATAACCATGTCC---------------------------AGAGGG | 27 bp deleted → no frameshift |
| 126 | CCCCCATCCAGAAACATAACC-----------------------CGGACTCTCCAGAGGG | 23 bp deleted → frameshift |
| 120 | CCCCCATCCAGAAACATAACC------------------------GGACTCTCCAGAGGG | 24 bp deleted → no frameshift |
| 120 | CCCCCATCCAGAAACAT------------------------CCCCGGACTCTCCAGAGGG | 24 bp deleted → no frameshift |
| 108 | CCCCC----------------------------------------GGACTCTCCAGAGGG | 40 bp deleted → frameshift |
| 103 | CCCC-----------------------------------------GGACTCTCCAGAGGG | 41 bp deleted → frameshift |
| 99 | CCCCCATCCAGAAACATAACCATGTCCT----------------------CTCCAGAGGG | 22 bp deleted → frameshift |
| 96 | CCCCCATCCAGAAACATAACCA---------------------------------GAGGG | 33 bp deleted → no frameshift |
| 94 | CCCCCATCCAGA---------------------------------------------GGG | 45 bp deleted → no frameshift |
| 90 | CCCCCATCCAGAAACATAACCATGTCCT------------------------CCAGAGGG | 24 bp deleted → no frameshift |
| 86 | CCCCCATCCAGAAACATAACCATGTC-------------------------TCCAGAGGG | 25 bp deleted → frameshift |
| 85 | CCCCC---------------------------------------CGGACTCTCCAGAGGG | 39 bp deleted → no frameshift |
| 74 | CCCCCATC----------------------------TCCATCCCCGGACTCTCCAGAGGG | 28 bp deleted → frameshift |
| 73 | CCC------------------------------------------GGACTCTCCAGAGGG | 42 bp deleted → no frameshift |
| 70 | CCCCCATCCAGAAACATAAC-----------------------------TCTCCAGAGGG | 29 bp deleted → frameshift |
| 69 | CCCCCATCC-----------------------------------CGGACTCTCCAGAGGG | 35 bp deleted → frameshift |
| 69 | CCCC-----------------------------------ATCCCCGGACTCTCCAGAGGG | 35 bp deleted → frameshift |
| 66 | CCCCCATCC------------------------------------GGACTCTCCAGAGGG | 36 bp deleted → no frameshift |
| 66 | CCC------------------------------------ATCCCCGGACTCTCCAGAGGG | 36 bp deleted → no frameshift |
| 62 | CC-------------------------------------ATCCCCGGACTCTCCAGAGGG | 37 bp deleted → frameshift |
| 60 | CCCCC--------------------------------------CCGGACTCTCCAGAGGG | 38 bp deleted → frameshift |
| 54 | CCCCCATCCAGAAAC----------------------------------TCTCCAGAGGG | 34 bp deleted → frameshift |
| 49 | CCCCCATCCAGA------------------------------------CTCTCCAGAGGG | 36 bp deleted → no frameshift |
| 46 | CC-------------------------------------------GGACTCTCCAGAGGG | 43 bp deleted → frameshift |
| 43 | CCCCCA-------------------------------------------------GAGGG | 49 bp deleted → frameshift |
| 42 | CCCCCATCCAGAAACA---------------------------------------GAGGG | 39 bp deleted → no frameshift |
| 34 | CCCCCATC-------------------------------------------TCCAGAGGG | 43 bp deleted → frameshift |
| 32 | CCCC--------------------------------------------------AGAGGG | 50 bp deleted → frameshift |
| 31 | CCC---------------------------------------------------AGAGGG | 51 bp deleted → no frameshift |
| 29 | CC----------------------------------------------------AGAGGG | 52 bp deleted → frameshift |
| 28 | CCCCCATCCAG-----------------------------------------------GG | 47 bp deleted → frameshift |