CRISPOR

DNA break repair outcome predictions

Guide: TCTTCTCAGCCAAGATCCCA GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TGCCTTGTCGCCTTAGCTCCTCTTCTCAGCCAAGATCCCAGGGAGCCTGGGATTAGGAGCTTACTTGGGGGTTTTCCCCC`	(Wild-type sequence)
314	`CCTTAGCTCCTCTTCTCAGCC----------------TGGGATTAGGAGCTTACTTGGGG`	`16 bp deleted → frameshift`
268	`CCTTAGCTCCTCTTCTCAGCCAAGATCCC--------TGGGATTAGGAGCTTACTTGGGG`	`8 bp deleted → frameshift`
255	`CCTTAGCTCCTCTTCTCAGCCAAGATCC---------TGGGATTAGGAGCTTACTTGGGG`	`9 bp deleted → no frameshift`
191	`CCTTAGCTCCTCTTCTCAGCCAAGA---------GCCTGGGATTAGGAGCTTACTTGGGG`	`9 bp deleted → no frameshift`
173	`CCTTAGCTCCTCTTCTCAGCCAAG-----------CCTGGGATTAGGAGCTTACTTGGGG`	`11 bp deleted → frameshift`
170	`CCTTAGCTCCTCTTCTCAGCCAAGAT-----------------TAGGAGCTTACTTGGGG`	`17 bp deleted → frameshift`
129	`CCTTAGCTCCT---------------------------GGGATTAGGAGCTTACTTGGGG`	`27 bp deleted → no frameshift`
117	`CCTTAGC-----------------------------CTGGGATTAGGAGCTTACTTGGGG`	`29 bp deleted → frameshift`
111	`CCTTAGCTCCTCTTCTCAGC------------------------------TTACTTGGGG`	`30 bp deleted → no frameshift`
99	`CCTTAGCTCCTCTTCTCAGCCAAG----------------------GAGCTTACTTGGGG`	`22 bp deleted → frameshift`
99	`CCTTAGCTCCTCTTCT----------------------GGGATTAGGAGCTTACTTGGGG`	`22 bp deleted → frameshift`
95	`CCTTAGCTCCTCTTCTCAGCCAAGA-----------------------GCTTACTTGGGG`	`23 bp deleted → frameshift`
87	`CCT-----------------------------------GGGATTAGGAGCTTACTTGGGG`	`35 bp deleted → frameshift`
86	`CCTTAGCTCCTCTTCTCAGCCAAG-------------------------CTTACTTGGGG`	`25 bp deleted → frameshift`
86	`CCTTAGCTCCTCT-------------------------GGGATTAGGAGCTTACTTGGGG`	`25 bp deleted → frameshift`
77	`CCTTAGCTCCTCTTCTCAG---------------------------GAGCTTACTTGGGG`	`27 bp deleted → no frameshift`
69	`CCTTAGCT-------------------------------------------TACTTGGGG`	`43 bp deleted → frameshift`
67	`CCTTAG----------------------------------------GAGCTTACTTGGGG`	`40 bp deleted → frameshift`
66	`CCTTAGCT------------------------------GGGATTAGGAGCTTACTTGGGG`	`30 bp deleted → no frameshift`
60	`CCTTAGCTCCTCTT--------------------------------------ACTTGGGG`	`38 bp deleted → frameshift`
52	`CCTTAGCTCCTCTTCT-----------------------------------TACTTGGGG`	`35 bp deleted → frameshift`
48	`CCTTAGCTCCTCTT------------------------------------------GGGG`	`42 bp deleted → no frameshift`
45	`CCTTA------------------------------------------------CTTGGGG`	`48 bp deleted → no frameshift`
44	`CCTTAGCTCCTCTT------------------------------AGGAGCTTACTTGGGG`	`30 bp deleted → no frameshift`
42	`CCTTAGCTCCTCTTCT---------------------------------------TGGGG`	`39 bp deleted → no frameshift`
40	`CCTTAGCTCCT----------------------------------------TACTTGGGG`	`40 bp deleted → frameshift`
33	`CCTTAGCTCCT--------------------------------------------TGGGG`	`44 bp deleted → frameshift`
29	`CCTT----------------------------------------------------GGGG`	`52 bp deleted → frameshift`
28	`CCTTAGCT-----------------------------------------------TGGGG`	`47 bp deleted → frameshift`

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