← return to the list of all guides
Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313948_F | TCGTCGGCAGCGTCAATTTGTCCGAAGCCGTGAG | 58.8 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313948_R | GTCTCGTGGGCTCGGCTCCTCCTCCTGGCCTCC | 60.4 | 1.00 |
| mm4_exon_Irs2/9530052E02Rik_chr8_11006612_F | Primer3: not found at this Tm | N.d. | 0.56 |
| mm4_exon_Irs2/9530052E02Rik_chr8_11006612_R | Primer3: not found at this Tm | N.d. | 0.56 |
| mm4_intron_Iqck_chr7_118931250_F | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4_intron_Iqck_chr7_118931250_R | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4_intergenic_Zfp317|Olfr857_chr9_19655950_F | Primer3: not found at this Tm | N.d. | 0.38 |
| mm4_intergenic_Zfp317|Olfr857_chr9_19655950_R | Primer3: not found at this Tm | N.d. | 0.38 |
| mm4_intergenic_Mia2|Ctage5_chr12_59111685_F | Primer3: not found at this Tm | N.d. | 0.38 |
| mm4_intergenic_Mia2|Ctage5_chr12_59111685_R | Primer3: not found at this Tm | N.d. | 0.38 |
| mm4_intron_Dtx4_chr19_12478353_F | TCGTCGGCAGCGTCCATGACCTTCCCCTCAACCC | 60.0 | 0.37 |
| mm4_intron_Dtx4_chr19_12478353_R | GTCTCGTGGGCTCGGCGGGTCTGTTATCCACTGGG | 59.8 | 0.37 |
| mm4_intergenic_Gm17130|Bre_chr5_32041174_F | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4_intergenic_Gm17130|Bre_chr5_32041174_R | Primer3: not found at this Tm | N.d. | 0.36 |
| mm3_intron_Suv420h1_chr19_3787547_F | Primer3: not found at this Tm | N.d. | 0.35 |
| mm3_intron_Suv420h1_chr19_3787547_R | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intergenic_Gm26341|Amd2_chr10_35461337_F | TCGTCGGCAGCGTCAAACTCATGACCCTGCTGCC | 60.6 | 0.34 |
| mm4_intergenic_Gm26341|Amd2_chr10_35461337_R | GTCTCGTGGGCTCGGGGTACTTTCATGAGGCAAAATGC | 58.8 | 0.34 |
| mm4_intron_Arfgap3_chr15_83303814_F | TCGTCGGCAGCGTCCAAGGACAGGGCTCTAGCAC | 60.1 | 0.34 |
| mm4_intron_Arfgap3_chr15_83303814_R | GTCTCGTGGGCTCGGCCATGGTTCCAGGCCTTTCT | 59.9 | 0.34 |
| mm4_intergenic_Zfp947|Gm4944_chr17_22174012_F | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4_intergenic_Zfp947|Gm4944_chr17_22174012_R | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4_intergenic_Vmn2r97|Vmn2r-ps118_chr17_18954079_F | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4_intergenic_Vmn2r97|Vmn2r-ps118_chr17_18954079_R | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4_intergenic_Vmn2r-ps117|Vmn2r97_chr17_18855493_F | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4_intergenic_Vmn2r-ps117|Vmn2r97_chr17_18855493_R | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4_intergenic_Cntn5|Gm25365_chr9_12545770_F | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4_intergenic_Cntn5|Gm25365_chr9_12545770_R | Primer3: not found at this Tm | N.d. | 0.33 |
| mm3_exon_Dag1_chr9_108206071_F | TCGTCGGCAGCGTCCTATTGCTGGCACTCCTCCC | 60.1 | 0.33 |
| mm3_exon_Dag1_chr9_108206071_R | GTCTCGTGGGCTCGGCTCACTTGACCTTGGCCACT | 59.8 | 0.33 |
| mm4_intron_Abca5_chr11_110321973_F | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_intron_Abca5_chr11_110321973_R | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_intron_Zfp787_chr7_6152335_F | TCGTCGGCAGCGTCGGTACACGGTGCTATAAGCTCA | 59.9 | 0.31 |
| mm4_intron_Zfp787_chr7_6152335_R | GTCTCGTGGGCTCGGTTACCCTGTCAGCTTGCCTG | 59.9 | 0.31 |
| mm4_intergenic_Dysf|Cyp26b1_chr6_84266440_F | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intergenic_Dysf|Cyp26b1_chr6_84266440_R | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intergenic_Gm11902|Gm11901_chr4_27492210_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intergenic_Gm11902|Gm11901_chr4_27492210_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm3_exon_B3galt5_chr16_96315955_F | TCGTCGGCAGCGTCTGGCCAAGCTAAAGATCCGG | 60.1 | 0.28 |
| mm3_exon_B3galt5_chr16_96315955_R | GTCTCGTGGGCTCGGGTAAGTGAGCAGGTCCTGGG | 59.7 | 0.28 |
| mm4_intergenic_Fgf16|Atrx_chrX_105787953_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Fgf16|Atrx_chrX_105787953_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_exon_Syt2_chr1_134745719_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_exon_Syt2_chr1_134745719_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_Ispd|Mir5099_chr12_36756576_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Ispd|Mir5099_chr12_36756576_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Speer7-ps1_chr5_15695108_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Speer7-ps1_chr5_15695108_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Rarb|Thrb_chr14_17222884_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Rarb|Thrb_chr14_17222884_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Gm17019|Gm21847_chr5_15512404_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Gm17019|Gm21847_chr5_15512404_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Gm15880|Crtc3_chr7_80647699_F | TCGTCGGCAGCGTCTAAGGCTAGGATCCGGCTGT | 60.1 | 0.23 |
| mm4_intergenic_Gm15880|Crtc3_chr7_80647699_R | GTCTCGTGGGCTCGGGGAGCGTAGGAGTGCAAACT | 60.0 | 0.23 |
| mm4_intergenic_Churc1/Fntb|Churc1_chr12_76776882_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Churc1/Fntb|Churc1_chr12_76776882_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Gm15446|1010001B22Rik_chr5_109953549_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Gm15446|1010001B22Rik_chr5_109953549_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Gm833|Gm25779_chr4_152998291_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Gm833|Gm25779_chr4_152998291_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Irx2|Irx4_chr13_72815151_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Irx2|Irx4_chr13_72815151_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Frk|Gm26341_chr10_34702523_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Frk|Gm26341_chr10_34702523_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_4930556G01Rik_chr4_30691259_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_4930556G01Rik_chr4_30691259_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_exon_Ahi1_chr10_20959774_F | TCGTCGGCAGCGTCCACTACCCAGATCGCACAGG | 60.1 | 0.19 |
| mm4_exon_Ahi1_chr10_20959774_R | GTCTCGTGGGCTCGGTGAATCATCTGCTGTCGGGG | 59.8 | 0.19 |
| mm4_intron_Rps6kc1_chr1_190809575_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_Rps6kc1_chr1_190809575_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Sox18|Tcea2_chr2_181673546_F | TCGTCGGCAGCGTCGGCTCAGATGTCAGGCTGTT | 60.0 | 0.19 |
| mm4_intergenic_Sox18|Tcea2_chr2_181673546_R | GTCTCGTGGGCTCGGCCAGCATCACTGTTGCCAAG | 59.7 | 0.19 |
| mm4_intergenic_Defb4|Defa-ps12_chr8_19203413_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Defb4|Defa-ps12_chr8_19203413_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Usp53_chr3_122963221_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Usp53_chr3_122963221_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Sppl3_chr5_115091184_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Sppl3_chr5_115091184_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm3_intergenic_Gm21614|Gm23249_chr12_92687321_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm3_intergenic_Gm21614|Gm23249_chr12_92687321_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm24780|Ttc14_chr3_33677680_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm24780|Ttc14_chr3_33677680_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Tgm7|Lcmt2_chr2_121115508_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Tgm7|Lcmt2_chr2_121115508_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm25736|Tmeff2_chr1_51055517_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm25736|Tmeff2_chr1_51055517_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3_intergenic_Gm22242|Ddx10_chr9_52853454_F | TCGTCGGCAGCGTCTGCATTGGCCTTAAGTGTGG | 58.7 | 0.16 |
| mm3_intergenic_Gm22242|Ddx10_chr9_52853454_R | GTCTCGTGGGCTCGGCCATCTGTCACGTCCGTCAA | 60.0 | 0.16 |
| mm4_intergenic_Gm25722|Gm10051_chr5_133436692_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm25722|Gm10051_chr5_133436692_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Cdh6|Gm9630_chr15_13671025_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Cdh6|Gm9630_chr15_13671025_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Trhr|Gm24903_chr15_44291994_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Trhr|Gm24903_chr15_44291994_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm23083|Gm24773_chr16_80660876_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm23083|Gm24773_chr16_80660876_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm3_intergenic_Stpg2|mmu-let-7j_chr3_139813672_F | TCGTCGGCAGCGTCTCAGTGATAATTGCTGCGAGGT | 60.0 | 0.15 |
| mm3_intergenic_Stpg2|mmu-let-7j_chr3_139813672_R | GTCTCGTGGGCTCGGGGGCTCCTTGCAGAATGTCT | 60.0 | 0.15 |
| mm4_intergenic_Banp|Gm22_chr8_122095066_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Banp|Gm22_chr8_122095066_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Ptprv_chr1_135116253_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Ptprv_chr1_135116253_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm13332|Armc3_chr2_19197235_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm13332|Armc3_chr2_19197235_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_mmu-mir-6404|Shank2_chr7_143962707_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_mmu-mir-6404|Shank2_chr7_143962707_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Lrsam1_chr2_32934080_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Lrsam1_chr2_32934080_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm24863|Gm24635_chr13_79865790_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm24863|Gm24635_chr13_79865790_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm3_intergenic_Fhl5|Ufl1_chr4_25243727_F | TCGTCGGCAGCGTCGCACTGTCAGGTTTTGCAGG | 59.9 | 0.14 |
| mm3_intergenic_Fhl5|Ufl1_chr4_25243727_R | GTCTCGTGGGCTCGGGGAGAGTGCAGAGAAACACTGA | 59.9 | 0.14 |
| mm4_intergenic_Gnaq|Gna14_chr19_16426165_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gnaq|Gna14_chr19_16426165_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gfod1|Sirt5_chr13_43337512_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gfod1|Sirt5_chr13_43337512_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm3_intron_Ttyh2_chr11_114697912_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm3_intron_Ttyh2_chr11_114697912_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Rcn1|4930527A07Rik_chr2_105418220_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Rcn1|4930527A07Rik_chr2_105418220_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm23838|Gm25233_chr5_6245617_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm23838|Gm25233_chr5_6245617_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm22882|Cxcl12_chr6_116988815_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm22882|Cxcl12_chr6_116988815_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Max|Gm22696_chr12_77040208_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Max|Gm22696_chr12_77040208_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm23385|Gm7803_chrX_50027493_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm23385|Gm7803_chrX_50027493_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm23986|Gm24929_chr8_51777005_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm23986|Gm24929_chr8_51777005_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_2610015P09Rik|Zdhhc23_chr16_43968279_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_2610015P09Rik|Zdhhc23_chr16_43968279_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Rftn1_chr17_50103684_F | TCGTCGGCAGCGTCTGCATTCCCCAAAGTCCACT | 59.5 | 0.13 |
| mm4_intron_Rftn1_chr17_50103684_R | GTCTCGTGGGCTCGGTGCTTTACTGGTTGGACAAGTG | 59.0 | 0.13 |
| mm4_intron_Kif6_chr17_49877698_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Kif6_chr17_49877698_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm25813|Gm23666_chr8_52845004_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm25813|Gm23666_chr8_52845004_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Slc8a1|Rpl31-ps25_chr17_81762660_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Slc8a1|Rpl31-ps25_chr17_81762660_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm8349|Arhgef26_chr3_61957359_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm8349|Arhgef26_chr3_61957359_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Trim29|Pvrl1_chr9_43579726_F | TCGTCGGCAGCGTCTACTGAGTGCAGCCATGAGC | 60.1 | 0.11 |
| mm4_intergenic_Trim29|Pvrl1_chr9_43579726_R | GTCTCGTGGGCTCGGGTCTCAGTGTGCATGGGGTC | 60.6 | 0.11 |
| mm4_intergenic_Plcl1|Hsfy2_chr1_55807409_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Plcl1|Hsfy2_chr1_55807409_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm23965|Gm23537_chr1_103486293_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm23965|Gm23537_chr1_103486293_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm3_intergenic_Adcy5|Gm25179_chr16_35223482_F | TCGTCGGCAGCGTCTGGCTCTTTGTCTGCCTCTG | 59.9 | 0.10 |
| mm3_intergenic_Adcy5|Gm25179_chr16_35223482_R | GTCTCGTGGGCTCGGTCCACTAGCACTCTTCCCAGA | 59.9 | 0.10 |
| mm4_intron_Zp2_chr7_120136719_F | TCGTCGGCAGCGTCTCCGAGCACTTTGGTAAGGC | 60.3 | 0.10 |
| mm4_intron_Zp2_chr7_120136719_R | GTCTCGTGGGCTCGGCTAGCTGCACCTCGCTTCTC | 60.5 | 0.10 |
| mm4_intergenic_Gm25644|Rpl31-ps18_chr19_51630797_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm25644|Rpl31-ps18_chr19_51630797_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm25800|Efna5_chr17_62513006_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm25800|Efna5_chr17_62513006_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Prdx6_chr1_161246272_F | TCGTCGGCAGCGTCATGGACAGCTGGAGGTAGCA | 60.6 | 0.09 |
| mm4_intron_Prdx6_chr1_161246272_R | GTCTCGTGGGCTCGGTCAGACACTGACTCTCCTGGT | 59.8 | 0.09 |
| mm3_exon_Efr3a_chr15_65868943_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm3_exon_Efr3a_chr15_65868943_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Coro2b_chr9_62459766_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Coro2b_chr9_62459766_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm3_intergenic_Cyp2d12|AC118710.1_chr15_82566127_F | TCGTCGGCAGCGTCGCAATGTTGGCATAGGTCCC | 59.2 | 0.09 |
| mm3_intergenic_Cyp2d12|AC118710.1_chr15_82566127_R | GTCTCGTGGGCTCGGAGCAACCCTGAGTTTGAAGGA | 59.5 | 0.09 |
| mm4_intergenic_Gm12414|Bnc2_chr4_84142188_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm12414|Bnc2_chr4_84142188_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Vwa8_chr14_79072308_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Vwa8_chr14_79072308_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Nipbl|Gm2310_chr15_8445034_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Nipbl|Gm2310_chr15_8445034_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_B3galnt2_chr13_13982893_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_B3galnt2_chr13_13982893_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intron_Gm15341_chr16_96109142_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intron_Gm15341_chr16_96109142_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Rab28|Nkx3-2_chr5_41724909_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Rab28|Nkx3-2_chr5_41724909_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Nav3|E2f7_chr10_110427667_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Nav3|E2f7_chr10_110427667_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Rnf144b|4931429P17Rik_chr13_47653771_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Rnf144b|4931429P17Rik_chr13_47653771_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Scg3_chr9_75654862_F | TCGTCGGCAGCGTCGATGGAAGAGAGCCAGAGCC | 59.8 | 0.08 |
| mm4_intron_Scg3_chr9_75654862_R | GTCTCGTGGGCTCGGTTAGGGCCAGCAAGACCATG | 60.0 | 0.08 |
| mm3_intron_Rsph3b_chr17_6930287_F | TCGTCGGCAGCGTCGGCTGGCTTACTTGGACCTC | 60.3 | 0.08 |
| mm3_intron_Rsph3b_chr17_6930287_R | GTCTCGTGGGCTCGGGCTTGGTCTCAGCACCATCT | 60.0 | 0.08 |
| mm3_intron_Rsph3a_chr17_7958953_F | TCGTCGGCAGCGTCCTGCGCCATGAAAGCAAAGT | 60.0 | 0.08 |
| mm3_intron_Rsph3a_chr17_7958953_R | GTCTCGTGGGCTCGGGGCTGGCTTACTTGGACCTC | 60.3 | 0.08 |
| mm3_intergenic_Trio|Dnah5_chr15_28058828_F | TCGTCGGCAGCGTCCCCTCAGCATCCCTCACTTC | 59.8 | 0.08 |
| mm3_intergenic_Trio|Dnah5_chr15_28058828_R | GTCTCGTGGGCTCGGTGAGAGCGAAGTGGCAGAAG | 60.0 | 0.08 |
| mm3_intergenic_Sdpr|Nabp1_chr1_51463282_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intergenic_Sdpr|Nabp1_chr1_51463282_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intergenic_Gm26796|4933426M11Rik_chr12_80780417_F | TCGTCGGCAGCGTCGCACCACTGGCTTACTTGGA | 60.2 | 0.08 |
| mm3_intergenic_Gm26796|4933426M11Rik_chr12_80780417_R | GTCTCGTGGGCTCGGTTCTTTGACTCCTGCGCCAT | 59.9 | 0.08 |
| mm3_intergenic_Foxi3|Gm23485_chr6_70968877_F | TCGTCGGCAGCGTCAGTGGCAAAGGAAGTGAGGG | 59.8 | 0.08 |
| mm3_intergenic_Foxi3|Gm23485_chr6_70968877_R | GTCTCGTGGGCTCGGTACTTCCACTCCCACAGGCT | 60.1 | 0.08 |
| mm3_exon_Rpl41_chr10_128548407_F | TCGTCGGCAGCGTCCCCTCAGCATCCCTCACTTC | 59.8 | 0.08 |
| mm3_exon_Rpl41_chr10_128548407_R | GTCTCGTGGGCTCGGTCAGGTTTGTGTGCCTTCCA | 59.7 | 0.08 |
| mm3_exon_Gm3699_chr10_43456444_F | TCGTCGGCAGCGTCCCCTCAGCATCCCTCACTTC | 59.8 | 0.08 |
| mm3_exon_Gm3699_chr10_43456444_R | GTCTCGTGGGCTCGGCGAAGTGGCGGAAGAAGAGA | 59.7 | 0.08 |
| mm3_exon_Gm10736_chr13_111593554_F | TCGTCGGCAGCGTCCCCTCAGCATCCCTCACTTC | 59.8 | 0.08 |
| mm3_exon_Gm10736_chr13_111593554_R | GTCTCGTGGGCTCGGCGAAGTGGCGGAAGAAGAGA | 59.7 | 0.08 |
| mm3_exon_Gm10180_chr9_25101424_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_exon_Gm10180_chr9_25101424_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_exon_Gm10076_chr14_105681902_F | TCGTCGGCAGCGTCTCGGTCTTAGCGCCATCTTC | 59.8 | 0.08 |
| mm3_exon_Gm10076_chr14_105681902_R | GTCTCGTGGGCTCGGCACAGGCTGGCTTACTTGGA | 59.9 | 0.08 |
| mm4_intron_Filip1l_chr16_57485999_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Filip1l_chr16_57485999_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Trit1|Gm12923_chr4_123073772_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Trit1|Gm12923_chr4_123073772_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm22448|Cadm2/1700010K23Rik_chr16_66442109_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm22448|Cadm2/1700010K23Rik_chr16_66442109_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Impg1|RP24-325L16.1_chr9_80659008_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Impg1|RP24-325L16.1_chr9_80659008_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm12520|2310081O03Rik_chr4_56202404_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm12520|2310081O03Rik_chr4_56202404_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Oxr1_chr15_41513904_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Oxr1_chr15_41513904_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Cab39l_chr14_59487580_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Cab39l_chr14_59487580_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Syt10|Gm24643_chr15_89852474_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Syt10|Gm24643_chr15_89852474_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Nfib|Zdhhc21_chr4_82707411_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Nfib|Zdhhc21_chr4_82707411_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm5391|Gm6806_chrX_68500820_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm5391|Gm6806_chrX_68500820_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm25460|Gm5263_chr1_146038298_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm25460|Gm5263_chr1_146038298_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm22480|Gm23096_chr3_49582575_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm22480|Gm23096_chr3_49582575_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm22223|Gm22715_chr8_102303006_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm22223|Gm22715_chr8_102303006_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm13935|Gm10794_chr2_110421400_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm13935|Gm10794_chr2_110421400_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm11221|Gm11222_chr4_69700586_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm11221|Gm11222_chr4_69700586_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Depdc1a|Rpe65_chr3_159581468_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Depdc1a|Rpe65_chr3_159581468_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm26207|Dmrt1_chr19_25487714_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm26207|Dmrt1_chr19_25487714_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Olfr400-ps1_chr11_74107833_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Olfr400-ps1_chr11_74107833_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_exon_Gm6263_chr13_74019673_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_exon_Gm6263_chr13_74019673_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_AI854517|Rhcg_chr7_79558189_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_AI854517|Rhcg_chr7_79558189_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Slc24a3|BC039771_chr2_145658130_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Slc24a3|BC039771_chr2_145658130_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm4487|Gm24073_chr14_114545036_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm4487|Gm24073_chr14_114545036_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm17421|Ighm_chr12_113371912_F | TCGTCGGCAGCGTCTAGAGCAGGCATGGAGGACT | 60.0 | 0.06 |
| mm4_intergenic_Gm17421|Ighm_chr12_113371912_R | GTCTCGTGGGCTCGGTGCTTCTTGTCCTGTCTAGGC | 59.7 | 0.06 |
| mm4_intron_Zfp407_chr18_84293670_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Zfp407_chr18_84293670_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Abl2_chr1_156570364_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Abl2_chr1_156570364_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Eif3a_chr19_60790563_F | TCGTCGGCAGCGTCCGTCAGCGAACTCCCTAGTG | 60.1 | 0.06 |
| mm4_exon_Eif3a_chr19_60790563_R | GTCTCGTGGGCTCGGCGTGGTTCCTTCCTTTCCGT | 60.2 | 0.06 |
| mm4_exon_Theg_chr10_79576582_F | TCGTCGGCAGCGTCGCTGGAGAGAAGGCTTGGTT | 59.9 | 0.06 |
| mm4_exon_Theg_chr10_79576582_R | GTCTCGTGGGCTCGGCCTCAACGAGGGCTACAACC | 60.3 | 0.06 |
| mm4_intergenic_Cck|Lyzl4_chr9_121513895_F | TCGTCGGCAGCGTCGGGGCTAGAAGACACTGAACA | 59.3 | 0.05 |
| mm4_intergenic_Cck|Lyzl4_chr9_121513895_R | GTCTCGTGGGCTCGGGTGGTGTGTACATCCTGCCA | 59.9 | 0.05 |
| mm4_intron_Crtac1_chr19_42358669_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Crtac1_chr19_42358669_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intergenic_Tmed4|Ogdh_chr11_6276874_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intergenic_Tmed4|Ogdh_chr11_6276874_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Cep97_chr16_55900416_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Cep97_chr16_55900416_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Camk2a_chr18_60970728_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Camk2a_chr18_60970728_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_St6galnac3|Gm10042_chr3_153778658_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_St6galnac3|Gm10042_chr3_153778658_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intron_Aqr_chr2_114146814_F | TCGTCGGCAGCGTCTGAGGTTAGCAAAGGGCCAG | 59.9 | 0.04 |
| mm3_intron_Aqr_chr2_114146814_R | GTCTCGTGGGCTCGGGCCCGAGATTCCTTGGTCAA | 60.0 | 0.04 |
| mm4_intergenic_Gm11506|Utp18_chr11_93820991_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm11506|Utp18_chr11_93820991_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Cdk18_chr1_132130849_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Cdk18_chr1_132130849_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm2_intergenic_Siva1|Akt1_chr12_112652423_F | TCGTCGGCAGCGTCCATGGCCACAGGGTCTATCC | 59.8 | 0.04 |
| mm2_intergenic_Siva1|Akt1_chr12_112652423_R | GTCTCGTGGGCTCGGTACCCTCTCCCCGAAGACAG | 60.0 | 0.04 |
| mm4_intron_Fbxo31_chr8_121575356_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Fbxo31_chr8_121575356_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Nrxn1_chr17_90357210_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Nrxn1_chr17_90357210_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_Cidea|Tubb6_chr18_67383977_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Cidea|Tubb6_chr18_67383977_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Edn2|Foxo6_chr4_120185799_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Edn2|Foxo6_chr4_120185799_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm11810|Rab2a_chr4_8535583_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm11810|Rab2a_chr4_8535583_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Gm13398_chr2_27038087_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Gm13398_chr2_27038087_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Zfp618_chr4_62981769_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Zfp618_chr4_62981769_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Ubl3|2210417A02Rik_chr5_148717108_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Ubl3|2210417A02Rik_chr5_148717108_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Incenp_chr19_9898622_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Incenp_chr19_9898622_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Ddx27_chr2_167034501_F | TCGTCGGCAGCGTCTCCCACAGCCTACCCATCTC | 60.6 | 0.03 |
| mm4_exon_Ddx27_chr2_167034501_R | GTCTCGTGGGCTCGGCTGCCCTCTACAGTGCAACA | 59.9 | 0.03 |
| mm4_intron_Lrit1_chr14_37056304_F | TCGTCGGCAGCGTCGGGATGATGGTGGCTGGAAA | 60.0 | 0.03 |
| mm4_intron_Lrit1_chr14_37056304_R | GTCTCGTGGGCTCGGTCATCAGCCATTTTGCAGGC | 59.4 | 0.03 |
| mm4_intron_l7Rn6_chr7_89928187_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_l7Rn6_chr7_89928187_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Gm25959|Gm13468_chr2_47557441_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Gm25959|Gm13468_chr2_47557441_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm23896|Gm24902_chr5_56827478_F | TCGTCGGCAGCGTCGAACTCCTGTTCCACGTGGT | 59.8 | 0.02 |
| mm4_intergenic_Gm23896|Gm24902_chr5_56827478_R | GTCTCGTGGGCTCGGACATGGCTTCTGAGAGCTGG | 59.7 | 0.02 |
| mm4_intergenic_Zscan4f|Vmn1r-ps53_chr7_11560735_F | TCGTCGGCAGCGTCTCCTCAGACAACATGGTGGC | 59.9 | 0.02 |
| mm4_intergenic_Zscan4f|Vmn1r-ps53_chr7_11560735_R | GTCTCGTGGGCTCGGGCTTAGCTGGCCTCCATAGG | 59.9 | 0.02 |
| mm4_intergenic_Zscan4f|Vmn1r-ps53_chr7_11444339_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Zscan4f|Vmn1r-ps53_chr7_11444339_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Mir297-1|Zscan4c_chr7_10975716_F | TCGTCGGCAGCGTCTCCTCAGACAACATGGTGGC | 59.9 | 0.02 |
| mm4_intergenic_Mir297-1|Zscan4c_chr7_10975716_R | GTCTCGTGGGCTCGGCTGCATGGCTTAGCTGACCT | 60.1 | 0.02 |
| mm4_intergenic_Gm23087|Zscan4f_chr7_11367722_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm23087|Zscan4f_chr7_11367722_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intron_Tnr_chr1_159783642_F | TCGTCGGCAGCGTCATCCTGGCTCACTGATGTCT | 58.1 | 0.02 |
| mm3_intron_Tnr_chr1_159783642_R | GTCTCGTGGGCTCGGGTGGAGGGTGGCTATTTGCT | 60.0 | 0.02 |
| mm4_intergenic_Gm24988|Gm6177_chr1_160879513_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm24988|Gm6177_chr1_160879513_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Klf6|Gm26259_chr13_5947818_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Klf6|Gm26259_chr13_5947818_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Vwa2_chr19_56897899_F | TCGTCGGCAGCGTCGTTCCCTCCCAGCTTCACC | 60.0 | 0.02 |
| mm4_exon_Vwa2_chr19_56897899_R | GTCTCGTGGGCTCGGACAGAGCCATTTCTGCCTCC | 60.0 | 0.02 |
| mm4_intron_Dars2_chr1_161046021_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Dars2_chr1_161046021_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Ndst1|Rps14_chr18_60756539_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Ndst1|Rps14_chr18_60756539_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Zscan4d|Gm26248_chr7_11253764_F | TCGTCGGCAGCGTCGCTTAGCTGGCCTCCATAGG | 59.9 | 0.01 |
| mm4_intergenic_Zscan4d|Gm26248_chr7_11253764_R | GTCTCGTGGGCTCGGCTGCAGACCAACTACCCCTC | 59.7 | 0.01 |
| mm4_intergenic_Vmn1r71|Zscan4b_chr7_10850822_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Vmn1r71|Zscan4b_chr7_10850822_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Jdp2_chr12_85603517_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Jdp2_chr12_85603517_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm26250|Gm11819_chr4_13403556_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm26250|Gm11819_chr4_13403556_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Ubr5|Odf1_chr15_38142684_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Ubr5|Odf1_chr15_38142684_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Rbfox3_chr11_118491540_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Rbfox3_chr11_118491540_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Ercc6l|Gm9103_chrX_102173098_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Ercc6l|Gm9103_chrX_102173098_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Chst8|Pepd_chr7_34837243_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Chst8|Pepd_chr7_34837243_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Folr4|Panx1_chr9_14955962_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Folr4|Panx1_chr9_14955962_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Kcnk1|Slc35f3_chr8_126155482_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Kcnk1|Slc35f3_chr8_126155482_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intergenic_Gm5973|Gm9915_chr1_41240417_F | TCGTCGGCAGCGTCCCAGCTTGAAATGGCACTGG | 59.7 | 0.00 |
| mm3_intergenic_Gm5973|Gm9915_chr1_41240417_R | GTCTCGTGGGCTCGGTCTACCGCACCTGCCAAATT | 59.9 | 0.00 |
| mm4_intron_Vat1l_chr8_114219758_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Vat1l_chr8_114219758_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Scaper_chr9_55647162_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Scaper_chr9_55647162_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Zbed6/Zc3h11a|Lax1_chr1_133667217_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Zbed6/Zc3h11a|Lax1_chr1_133667217_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Sorbs1|Aldh18a1_chr19_40493301_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Sorbs1|Aldh18a1_chr19_40493301_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Plcl1|Hsfy2_chr1_55952930_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Plcl1|Hsfy2_chr1_55952930_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Hk2|Sema4f_chr6_82783438_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Hk2|Sema4f_chr6_82783438_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313948 |
AATTTGTCCGAAGCCGTGAGCAGCCCGCTGCCTGAACCGCCAGAGAAGATGGTGCCCCACGGTTGCCTGAGCCCGCGAGC CGGCCCTCCGACTTCCCGGGAGCGTGGCGGGGGAGGCCAGGAGGAGGAG |
| mm4_intron_Dtx4_chr19_12478353 |
CATGACCTTCCCCTCAACCCGCCTAAAGCACCAGAGAAAATGGTCTCAAGAGGGAAAACTTACCCAGTGGATAACAGACC CG |
| mm4_intergenic_Gm26341|Amd2_chr10_35461337 |
AAACTCATGACCCTGCTGCCTATTTACCTCAACCTCCAGAGAAGTTGGAAGAGGTAAAAACACACAGAAAGAAATTACTC TCTATGAAAAACGTAACTTCATATGCATTTTGCCTCATGAAAGTACC |
| mm4_intron_Arfgap3_chr15_83303814 |
CAAGGACAGGGCTCTAGCACATGGGTTGAACTTCCAGAGAAGAGGGGGGCATGGGGGCAGCCAAGATGAGGAAATGACAG AGAGCAAACAATCAGAAAGGCCTGGAACCATGG |
| mm3_exon_Dag1_chr9_108206071 |
CTATTGCTGGCACTCCTCCCCAGCCACAGGCCCACGGCCCAGGACTGGAGCGCCAGAGAAGAGGGGCAGGGTATATGGGG TAGGGCTGTGGACTGTTGGGACAAAGTGGCCAAGGTCAAGTGAG |
| mm4_intron_Zfp787_chr7_6152335 |
GGTACACGGTGCTATAAGCTCATAAGCTTAGATCCTGAGCGCCTGAACTGCCAGGGAAAGAGGCCAGAGGACAATCCCTA CAGGCAAGCTGACAGGGTAA |
| mm3_exon_B3galt5_chr16_96315955 |
TGGCCAAGCTAAAGATCCGGCCGGAGGAGCTGCACACCAAACAGACCTTCTTCCCTGGCGGTTTACGCTTTTCCGTGTGC CGCTTTCAGAAAATTGTGGCATGCCATTTTATGAAGCCCCAGGACCTGCTCACTTAC |
| mm4_intergenic_Gm15880|Crtc3_chr7_80647699 |
TAAGGCTAGGATCCGGCTGTGGCTGCTCAGCAGCAGAGCACAGCACGCACAAGCTCTGGGTGTAATCCCTAGTGCCACAG AAACGCGTGAAACACCAGAGAAGGGGGATGCAGTTTGCACTCCTACGCTCC |
| mm4_exon_Ahi1_chr10_20959774 |
CACTACCCAGATCGCACAGGAAATGGAGCCAGAAACTCCAGAGAAGGTGGATTCTGCACAAGAGAAAGTCAGAGGCAAGA CCCCGACAGCAGATGATTCA |
| mm4_intergenic_Sox18|Tcea2_chr2_181673546 |
GGCTCAGATGTCAGGCTGTTATATCCTGTGAGCTAGCTGGGCAAGAGCCTGCTGAGTGCTCCCTTTTCTCTGTAGGGTCA GGCTAACCCTCACTGCCCACTGCTTGGCAACAGTGATGCTGG |
| mm3_intergenic_Gm22242|Ddx10_chr9_52853454 |
TGCATTGGCCTTAAGTGTGGTTCATTTGCTGTGTTACACAGAATTGTCTGAACTTCCAGAGAAGAGAGTTCTGCCCTTGG AGTCAAACTTCACAGCAGCTTGAAGTTTGACGGACGTGACAGATGG |
| mm3_intergenic_Stpg2|mmu-let-7j_chr3_139813672 |
TCAGTGATAATTGCTGCGAGGTAAAAATGTCTTTCATTCCTGAGCAGCCAGAGAAGAGAGTAGCAGTGATAGCTTGATGA CATGAGACATTCTGCAAGGAGCCC |
| mm3_intergenic_Fhl5|Ufl1_chr4_25243727 |
GCACTGTCAGGTTTTGCAGGACGTTCCTCTGGCCAGGCCTATTCTTGGAACCTACTTCCTTACTTTCTCTAAGTTAGACT TCTGGTTTCCTCTCTTCTCTGGCGGTTTATTCTTTGTTCAGTGTTTCTCTGCACTCTCC |
| mm4_intron_Rftn1_chr17_50103684 |
TGCATTCCCCAAAGTCCACTTAGTTGCCAAAAACTTTGTGTTCTCCTTCCAAGTTTAAAATAGTCCTTCTTCTGTGGTGG TTCTAGCCAGTCTACTTCACTTGTCCAACCAGTAAAGCA |
| mm4_intergenic_Trim29|Pvrl1_chr9_43579726 |
TACTGAGTGCAGCCATGAGCCTATGAGCCTGAGCCTCCACAGAACAAGGGTCAGATTATCACCATGATAGCTTACCAAGA CCTGTATGATAGTCTACATACAGAGGCCCGTGACCCCATGCACACTGAGAC |
| mm3_intergenic_Adcy5|Gm25179_chr16_35223482 |
TGGCTCTTTGTCTGCCTCTGTACAGAGCAGAAGTCCCTCCCATGCCTGAAACTCCAGAGAAGGCAGCTTCACCTGGGAAT GATGGAAAGTCACCGGATACAGAGACTACTTCTGGGAAGAGTGCTAGTGGA |
| mm4_intron_Zp2_chr7_120136719 |
TCCGAGCACTTTGGTAAGGCATACTCAGACCAGACTTACTAATCCCAGGCAATTTGAGGAGAAACTGAGAAGGCAGCTTT CAATGATTGGCCTGACCTGCTAGAGATGAGGGAATAAAGAGAAGCGAGGTGCAGCTAG |
| mm4_intron_Prdx6_chr1_161246272 |
ATGGACAGCTGGAGGTAGCATCCTGAAAAGCCAGAGCAGAGGGAAGGTTTAGAGAGATGAACTGGGCTGAATTCTGATAA CCAGGAGAGTCAGTGTCTGA |
| mm3_intergenic_Cyp2d12|AC118710.1_chr15_82566127 |
GCAATGTTGGCATAGGTCCCCAAAACCCTGAACCGCCATAGATGATGGTTAAGACAGCTCTCACAATTACATAACCTGAT TCCTTCAAACTCAGGGTTGCT |
| mm4_intron_Scg3_chr9_75654862 |
GATGGAAGAGAGCCAGAGCCAAGACTCAGCCAGCTTCTCTGGCTGTTCTGGTCTTCAATGCTATAAGAGAAATACCCCTT CTTGCTCATGGTCTTGCTGGCCCTAA |
| mm3_intron_Rsph3b_chr17_6930287 |
GGCTGGCTTACTTGGACCTCTGTCTCATCTTTCTTTCTTCTCTTGCGCTTCAGCCTGCGCATTCTCTTCTTCCGCCACTT TGCTTTCATGGCGCAGGAATCTTCAAGGAAGATGGTGCTGAGACCAAGC |
| mm3_intron_Rsph3a_chr17_7958953 |
CTGCGCCATGAAAGCAAAGTGGCAGAAGAAGAGAATGCGCAGGCTGAAGCGCAAGAGAAGAAAGAAAGATGAGACAGAGG TCCAAGTAAGCCAGCC |
| mm3_intergenic_Trio|Dnah5_chr15_28058828 |
CCCTCAGCATCCCTCACTTCTGCTCCTGCAGGTGTCGTGGGTGCACCGCTGGCTTACTTGGACCTCTGCCTCATCTTTCT TCTCTTGCGCTTCAGCCTGCACATTCTCTTCTGCCACTTCGCTCTCA |
| mm3_intergenic_Gm26796|4933426M11Rik_chr12_80780417 |
GCACCACTGGCTTACTTGGACCTCTGCCTCATCTTTCTTCTCTTGCGCTTCAGCCTGCGCATTCTCTTCTTCTGCCACTC ATGGCGCAGGAGTCAAAGAA |
| mm3_intergenic_Foxi3|Gm23485_chr6_70968877 |
AGTGGCAAAGGAAGTGAGGGCGCAGGCTGAAGCGCAAGAGAAGAGAGATGAGGTAGAGGTCCAAGTAAATTAGCTTGTGC ACCCATGAAGCCTGTGGGAGTGGAAGTA |
| mm3_exon_Rpl41_chr10_128548407 |
CCCTCAGCATCCCTCACTTCTGCTCCTGCAGGCGTCGTAGGTGCACGGGCTGGCTTACTTGGACCTCTGCCTCATCTTTC TTCTCTTGCGCTTCAGCCTGGAATGGAAGGCACACAAACCTGA |
| mm3_exon_Gm3699_chr10_43456444 |
CCCTCAGCATCCCTCACTTCTGCTCCTGCAGGCGTCGTGGGTGCACCGGCAGGCTTACTTGGACCTCTGCCTCATCTTTC TTCTCTTGCGCTTCAGCCTGCGCATTCTCTTCTTCCGCCACTTCG |
| mm3_exon_Gm10736_chr13_111593554 |
CCCTCAGCATCCCTCACTTCTGCTCCTGCAGGCGTCGTAGGTGCACGGGCTGGCTTACTTGGACCTCTGCCTCATCTTTC TTCTCTTGCGCTTCAGCCTGCGCATTCTCTTCTTCCGCCACTTCG |
| mm3_exon_Gm10076_chr14_105681902 |
TCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTGGCGGAAGAAGAGAATGCGCAGGCTGAAGC GCAAGAGAAGAAAGATGAGGCAGAGGTCCAAGTAAGCCAGCCTGTG |
| mm4_intergenic_Gm17421|Ighm_chr12_113371912 |
TAGAGCAGGCATGGAGGACTCCTGGATAATCTAGGTCATACCACTAGGTTCAATATGTCTATTGAACCCAGGGACTAAGC CAGAACAGTTAGAGAAGAGGGCTATTGGCCTAGACAGGACAAGAAGCA |
| mm4_exon_Eif3a_chr19_60790563 |
CGTCAGCGAACTCCCTAGTGGCCTGGACCGGGAGAGAAGGCGGAGGGGAACTAGCGCGAGCTTCCACGCGCCTCGCCAGC AGATGCCCGCGCCCAGCCGGCCAGAGACGGAAAGGAAGGAACCACG |
| mm4_exon_Theg_chr10_79576582 |
GCTGGAGAGAAGGCTTGGTTGTCACACTTTCTTGGTGATGTATTTGGGGGTGGCAAGCTCATAAATTCGAGGAGATGCCT GAGCCACCAGAGAGGCTGGGGAGATGTAGTATGGGTTGTAGCCCTCGTTGAGG |
| mm4_intergenic_Cck|Lyzl4_chr9_121513895 |
GGGGCTAGAAGACACTGAACATCCTCCCAAACCATGGCATGAATTGCTAGGTGAGAAGTAAGAATCCATCTTCTCTGAGG GTACGGGCCCAGAGCAAGTCCTTCATGGCAGGATGTACACACCAC |
| mm3_intron_Aqr_chr2_114146814 |
TGAGGTTAGCAAAGGGCCAGCCCGCAGCCCGCAGCGCCAGAGAAGAAAGAAAAGCCCACAGCGAAGCCTAGGAGACAATG CTGTTCTTTCCATGTCTTACCTGAGAGGCCCAAAAAACTTGACCAAGGAATCTCGGGC |
| mm2_intergenic_Siva1|Akt1_chr12_112652423 | CATGGCCACAGGGTCTATCCCCTATCTGCTCTGGTGGTTCAGGCTAGGCCAGGCCTGTCTTCGGGGAGAGGGTA |
| mm4_exon_Ddx27_chr2_167034501 |
TCCCACAGCCTACCCATCTCATTCTTCATCTGTCTTACAGCCCTTCCTTTGAAGAGAGGAAACAGTCAGGTCTGCCCCGC CAGAGGAGAGGGAACTTCAAATCTAAGTCCAGGTGATGTTGCACTGTAGAGGGCAG |
| mm4_intron_Lrit1_chr14_37056304 |
GGGATGATGGTGGCTGGAAAGGAAGCAGAACCCCCAGAGAAGAAAGCACTCCATCTTTGATGATTTAACTCACATGGGAA GTAAATTTCCCAGGAAGTAAGACAGGCTGGTACTTAATCAGCCTGCAAAATGGCTGATGA |
| mm4_intergenic_Gm23896|Gm24902_chr5_56827478 |
GAACTCCTGTTCCACGTGGTGGCTGCTGGGTGGGATCCACTCCAGTGTGAATCCCTCTACTCTGGTGGTGCTGGCTAGGA GGCTGGCACAGTGTCCAGCTCTCAGAAGCCATGT |
| mm4_intergenic_Zscan4f|Vmn1r-ps53_chr7_11560735 |
TCCTCAGACAACATGGTGGCACCATCTCCGTCTTCGATGGGGGTTCAGACTCCAGCTCAGAGTCCAAGCCTGAAGAGCCC CAGCAGGAAGAAACTCCCTATGGAGGCCAGCTAAGC |
| mm4_intergenic_Mir297-1|Zscan4c_chr7_10975716 |
TCCTCAGACAACATGGTGGCACCATCTCCGTCTTCGATGGGGGTTCAGACTCCAGCTCAGAGTCCAAGCCTGAAGAGCCC CAGCAGGAAGAAACTCCCTATGGAGGTCAGCTAAGCCATGCAG |
| mm3_intron_Tnr_chr1_159783642 |
ATCCTGGCTCACTGATGTCTGCCTGAACCGCCATAGTAAATGGTAGAAACTTCAAGGTGGTTTAGAACACTGTCATCTAA CTGCACTCCATCTGAAAGATGTGCCAAAGAACATGTAGCAAATAGCCACCCTCCAC |
| mm4_exon_Vwa2_chr19_56897899 |
GTTCCCTCCCAGCTTCACCCCCCGCCCCCCATGACTTCTTTCTTGTTTCTCCCCCTGTCCTCAGAGGTGGGCGCACCGAG ACGGGCCTAGCCCTGAAACGCCTGAGCAGAGGGTTCCCCGGAGGCAGAAATGGCTCTGT |
| mm4_intergenic_Zscan4d|Gm26248_chr7_11253764 |
GCTTAGCTGGCCTCCATAGGGAGTTTCTTCCTGCTGGGGCTCTTCAGGCTCGGACTCGGAGCTGGAGTCTGAACCCCCAT TGAAGACGGAGACGGTGCCACCCTGTTGTCTGAGGAGGGGTAGTTGGTCTGCAG |
| mm3_intergenic_Gm5973|Gm9915_chr1_41240417 |
CCAGCTTGAAATGGCACTGGATGGGTGCTTATGTTCTTCGTCTCCTCTGCTCTGGCGCTTCATGCATTTTTCATCTCCTG CTAATATTTATTCAGCACCACAATTTGGCAGGTGCGGTAGA |