CRISPOR

DNA break repair outcome predictions

Guide: GGGAGGCCAGGAGGAGGAGC CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`ACTTCCCGGGAGCGTGGCGGGGGAGGCCAGGAGGAGGAGCCGGTCGATGGACTAGCAGGCAGTGCTGCAGGGCTGGGCGC`	(Wild-type sequence)
296	`AGCGTGGCGGGGGAGGCCAGGAGGAGG------TCGATGGACTAGCAGGCAGTGCTGCAG`	`6 bp deleted → no frameshift`
261	`AGCGTGGCGGGGGAGGCCAGGAGGAGGA-------------CTAGCAGGCAGTGCTGCAG`	`13 bp deleted → frameshift`
255	`AGCGTGGCGGGGGAGGCCAGGAGG---------TCGATGGACTAGCAGGCAGTGCTGCAG`	`9 bp deleted → no frameshift`
224	`AGCGTGGCGGGGGAGGCCAGGAGGAGGAGC----------------AGGCAGTGCTGCAG`	`16 bp deleted → frameshift`
224	`AGCGTGGCGGGGGAGGCCAGGAGGA----------------CTAGCAGGCAGTGCTGCAG`	`16 bp deleted → frameshift`
219	`AGCGTGGCGGGGGAGGCCAGG------------TCGATGGACTAGCAGGCAGTGCTGCAG`	`12 bp deleted → no frameshift`
193	`AGCGTGGCGGGGGAGGCCAGGA-------------------CTAGCAGGCAGTGCTGCAG`	`19 bp deleted → frameshift`
191	`AGCGTGGCGGGGGAGGCCAGGAGGAGGA---------TGGACTAGCAGGCAGTGCTGCAG`	`9 bp deleted → no frameshift`
190	`AGCGTGGCGG-----------------------TCGATGGACTAGCAGGCAGTGCTGCAG`	`23 bp deleted → frameshift`
172	`AGCGTGGCGGGGGAGGCCAGG----------------------------CAGTGCTGCAG`	`28 bp deleted → frameshift`
170	`AGCGTGGCGGGGGAGG-----------------TCGATGGACTAGCAGGCAGTGCTGCAG`	`17 bp deleted → frameshift`
166	`AGCGTGGCGGGGGAGGCCAGGAGGAGG----------------------CAGTGCTGCAG`	`22 bp deleted → frameshift`
164	`AGCGTGGCGGGGGAGGCCAGGAGGA------------TGGACTAGCAGGCAGTGCTGCAG`	`12 bp deleted → no frameshift`
147	`AGCGTGGCGGGGGAGGCCAGGAGGAGGAGC--------------------AGTGCTGCAG`	`20 bp deleted → frameshift`
147	`AGCGTGGCGGGGG--------------------TCGATGGACTAGCAGGCAGTGCTGCAG`	`20 bp deleted → frameshift`
143	`AGCGTGGCGGGGGAGGCCAGGAGG-------------------------CAGTGCTGCAG`	`25 bp deleted → frameshift`
141	`AGCGTGGCGGGGGAGGCCAGGA---------------TGGACTAGCAGGCAGTGCTGCAG`	`15 bp deleted → no frameshift`
140	`AGCGTGGCGGGG---------------------TCGATGGACTAGCAGGCAGTGCTGCAG`	`21 bp deleted → no frameshift`
134	`AGCGTGGCGGGGGAGGC---------------------------------AGTGCTGCAG`	`33 bp deleted → no frameshift`
133	`AGCGTGGCGGG----------------------TCGATGGACTAGCAGGCAGTGCTGCAG`	`22 bp deleted → frameshift`
129	`AGCGTGGCGGGGGA---------------------------CTAGCAGGCAGTGCTGCAG`	`27 bp deleted → no frameshift`
120	`AGCGTGGCGGGGGAGG------------------------ACTAGCAGGCAGTGCTGCAG`	`24 bp deleted → no frameshift`
116	`AGCGTGGCGGGGGAGGCCAGGAGGAGGAG-------------------GCAGTGCTGCAG`	`19 bp deleted → frameshift`
116	`AGCGTGGCGGGGGAGGCCAGGAGGAG-------------------CAGGCAGTGCTGCAG`	`19 bp deleted → frameshift`
114	`AGCGTGGCGGGGGAGGCCAGGAGGAGGAGC-------------------------TGCAG`	`25 bp deleted → frameshift`
109	`AGCGTGG--------------------------TCGATGGACTAGCAGGCAGTGCTGCAG`	`26 bp deleted → frameshift`
103	`AGCGTGGCG---------------------------ATGGACTAGCAGGCAGTGCTGCAG`	`27 bp deleted → no frameshift`
101	`AGCGTGGCGGGGGAGGCCAG--------------------------------TGCTGCAG`	`32 bp deleted → frameshift`
99	`AGCGTGGCGGGGGAGGCCAGGAG----------------------CAGGCAGTGCTGCAG`	`22 bp deleted → frameshift`
98	`AGCGTGGCGGGGGAGGCCAGGAGGAGGAGC----------------------------AG`	`28 bp deleted → frameshift`
98	`AGCGTGGCGGGG----------------------------ACTAGCAGGCAGTGCTGCAG`	`28 bp deleted → frameshift`
98	`AGCG----------------------------GTCGATGGACTAGCAGGCAGTGCTGCAG`	`28 bp deleted → frameshift`
96	`AGCGTGG---------------------------------ACTAGCAGGCAGTGCTGCAG`	`33 bp deleted → no frameshift`
95	`AGCGTGGCGGGGGAGGCCAGGAGGAGGAG-----------------------TGCTGCAG`	`23 bp deleted → frameshift`
95	`AGCGTGGCGGGGGA-----------------------TGGACTAGCAGGCAGTGCTGCAG`	`23 bp deleted → frameshift`
94	`AGCGTGGCGGGGGAGGC-----------------------------AGGCAGTGCTGCAG`	`29 bp deleted → frameshift`
94	`AGCGTGGCGGG-----------------------------ACTAGCAGGCAGTGCTGCAG`	`29 bp deleted → frameshift`
89	`AGCGTGGCGG------------------------------ACTAGCAGGCAGTGCTGCAG`	`30 bp deleted → no frameshift`
86	`AGCGTGGCGGGGGAGGCCAG-------------------------CAGGCAGTGCTGCAG`	`25 bp deleted → frameshift`
81	`AGCGTGGCGGGGGAGGCCAGGAGGAG--------------------------TGCTGCAG`	`26 bp deleted → frameshift`
80	`AGCG--------------------------------ATGGACTAGCAGGCAGTGCTGCAG`	`32 bp deleted → frameshift`
73	`AGCGTGGC------------------------------------------AGTGCTGCAG`	`42 bp deleted → no frameshift`
70	`AGCGTGGCGGGGGAGGCCAGGAG-----------------------------TGCTGCAG`	`29 bp deleted → frameshift`
70	`AGCGT-----------------------------CGATGGACTAGCAGGCAGTGCTGCAG`	`29 bp deleted → frameshift`
67	`AGCGTGGCGGGGGAGGCCAG----------------------------------------`	`40 bp deleted → frameshift`
66	`AGCGTGGCGGGGGAG------------------------------CAGGCAGTGCTGCAG`	`30 bp deleted → no frameshift`
66	`AGCGTGGCGGGGG------------------------------------CAGTGCTGCAG`	`36 bp deleted → no frameshift`
63	`AGCGTGGCGGGGGAGGCCAGGAGGAGGAG-------------------------------`	`31 bp deleted → frameshift`
62	`AGCGTGGCGGGG-------------------------------------CAGTGCTGCAG`	`37 bp deleted → frameshift`
60	`AGCGTGGCGGGGGAGGC--------------------------------------TGCAG`	`38 bp deleted → frameshift`
60	`AGCGTGGCGGG--------------------------------------CAGTGCTGCAG`	`38 bp deleted → frameshift`
60	`AGCGTGGC--------------------------------------AGGCAGTGCTGCAG`	`38 bp deleted → frameshift`
58	`AGC-------------------------------------------AGGCAGTGCTGCAG`	`43 bp deleted → frameshift`
56	`AGCGTGGCGG---------------------------------------CAGTGCTGCAG`	`39 bp deleted → no frameshift`
54	`AGCGTGGCGGGGGAGGCCAGGAGGAG----------------------------------`	`34 bp deleted → frameshift`
51	`AGCGTGGCGGGGGAGGC-----------------------------------------AG`	`41 bp deleted → frameshift`
47	`AGCGTGGCGGGGGAGGCCAGGAG-------------------------------------`	`37 bp deleted → frameshift`
47	`AGCGTGGCGGGGGAG-------------------------------------TGCTGCAG`	`37 bp deleted → frameshift`
45	`AGCGTG------------------------------------------------CTGCAG`	`48 bp deleted → no frameshift`
38	`AGCGTGGC-----------------------------------------------TGCAG`	`47 bp deleted → frameshift`
38	`AGC-----------------------------------------------AGTGCTGCAG`	`47 bp deleted → frameshift`
32	`AGCGTGGC--------------------------------------------------AG`	`50 bp deleted → frameshift`
31	`AGCGTGGCGGGGGAG---------------------------------------------`	`45 bp deleted → no frameshift`
30	`AG----------------------------------------------GCAGTGCTGCAG`	`46 bp deleted → frameshift`
29	`AGC----------------------------------------------------TGCAG`	`52 bp deleted → frameshift`
25	`AGC-------------------------------------------------------AG`	`55 bp deleted → frameshift`
24	`AG--------------------------------------------------TGCTGCAG`	`50 bp deleted → frameshift`
23	`AGCGTG---------------------------------------------------CAG`	`51 bp deleted → no frameshift`
16	`AG----------------------------------------------------------`	`58 bp deleted → frameshift`

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