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The following table lists possible deletions.
Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.
| mh- Score | Sequence | Effect |
|---|---|---|
| CATGTCCTTTCGGCTCTCCATCCCCGGACTCTCCAGAGGGGGGGAGGGGGAAAACCCCCAAGTAAGCTCCTAATCCCAGG | (Wild-type sequence) | |
| 603 | CGGCTCTCCATCCCCGGACTCTCCAGAGGG--------GGAAAACCCCCAAGTAAGCTCC | 8 bp deleted → frameshift |
| 516 | CGGCTCTCCATCCCCGGACTCTCCAGAGGG---GAGGGGGAAAACCCCCAAGTAAGCTCC | 3 bp deleted → no frameshift |
| 491 | CGGCTCTCCATCCCCGGACTCTCCAGAGGG----AGGGGGAAAACCCCCAAGTAAGCTCC | 4 bp deleted → frameshift |
| 382 | CGGCTCTCCATCCCCGGACTCTCCAGAGGG---------GAAAACCCCCAAGTAAGCTCC | 9 bp deleted → no frameshift |
| 364 | CGGCTCTCCATCCCCGGACTCTCCAGAGGG----------AAAACCCCCAAGTAAGCTCC | 10 bp deleted → frameshift |
| 362 | CGGCTCTCCATCCCCGGACTCTCCAGAGGG--GGAGGGGGAAAACCCCCAAGTAAGCTCC | 2 bp deleted → frameshift |
| 311 | CGGCTCTCCATCCCCGGACTCTCCAGAGG-----AGGGGGAAAACCCCCAAGTAAGCTCC | 5 bp deleted → frameshift |
| 282 | CGGCTCTCCATCCCCGGACTCTCCAGAGGG-------GGGAAAACCCCCAAGTAAGCTCC | 7 bp deleted → frameshift |
| 230 | CGGCTCTCCATCCCCGGACTCTCCAGAGG-----------AAAACCCCCAAGTAAGCTCC | 11 bp deleted → frameshift |
| 213 | CGGCTCTCCATCCCCGGA-----------------GGGGGAAAACCCCCAAGTAAGCTCC | 17 bp deleted → frameshift |
| 188 | CGGCTCTCCATCCCCGG---------------GGAGGGGGAAAACCCCCAAGTAAGCTCC | 15 bp deleted → no frameshift |
| 182 | CGGCTCTCCATCCCCGGACTCTCCAG----------GGGGAAAACCCCCAAGTAAGCTCC | 10 bp deleted → frameshift |
| 179 | CGGCTCTCCATCCCCGG----------------GAGGGGGAAAACCCCCAAGTAAGCTCC | 16 bp deleted → frameshift |
| 158 | CGGCTCTCCATCCCCGGA-----------------------AAACCCCCAAGTAAGCTCC | 23 bp deleted → frameshift |
| 153 | CGGCTCTCCATCCCC---------------------------------CAAGTAAGCTCC | 33 bp deleted → no frameshift |
| 149 | CGGCTCTCCATCCCCGGACTCTCCAGA--------------AAACCCCCAAGTAAGCTCC | 14 bp deleted → frameshift |
| 147 | CGGCTCTCCATCCCCGG--------------------GGGAAAACCCCCAAGTAAGCTCC | 20 bp deleted → frameshift |
| 146 | CGGCTCTCCATCCCC----------------------------------AAGTAAGCTCC | 34 bp deleted → frameshift |
| 143 | CGGCTCTCCATCCCCGGACTCTCCA-------------------------AGTAAGCTCC | 25 bp deleted → frameshift |
| 140 | CGGCTCTCCATCCCCGG---------------------GGAAAACCCCCAAGTAAGCTCC | 21 bp deleted → no frameshift |
| 133 | CGGCTCTCCATCCCCGGACTCTCC----------------------CCCAAGTAAGCTCC | 22 bp deleted → frameshift |
| 133 | CGGCTCTCCATCCCCGG----------------------GAAAACCCCCAAGTAAGCTCC | 22 bp deleted → frameshift |
| 126 | CGGCTCTCCATCCCCGGACTCTCC-----------------------CCAAGTAAGCTCC | 23 bp deleted → frameshift |
| 121 | CGGCTCTCCATCCCC--------------------------------CCAAGTAAGCTCC | 32 bp deleted → frameshift |
| 120 | CGGCTCTCCATCCCCGGACTCTCC------------------------CAAGTAAGCTCC | 24 bp deleted → no frameshift |
| 115 | CGGCTCTCCATCCCCGGACTCTCC------------------------------------ | 36 bp deleted → no frameshift |
| 104 | CGGCTCTCCATCCC-----------------------------------AAGTAAGCTCC | 35 bp deleted → frameshift |
| 94 | CGG-----------------------------GGAGGGGGAAAACCCCCAAGTAAGCTCC | 29 bp deleted → frameshift |
| 90 | CGGCTCTCCATCCCCGGACTCTCCAGAG------------------------TAAGCTCC | 24 bp deleted → no frameshift |
| 89 | CGG------------------------------GAGGGGGAAAACCCCCAAGTAAGCTCC | 30 bp deleted → no frameshift |
| 84 | CGGCTCTCCATCCCC-------------------------------CCCAAGTAAGCTCC | 31 bp deleted → frameshift |
| 84 | CGG-------------------------------AGGGGGAAAACCCCCAAGTAAGCTCC | 31 bp deleted → frameshift |
| 81 | CGGCTCTCCATCCCCGGACTCTCCAG--------------------------TAAGCTCC | 26 bp deleted → frameshift |
| 81 | CGGCTCTCCATCCCCGGAC--------------------------CCCCAAGTAAGCTCC | 26 bp deleted → frameshift |
| 75 | CGGCTCTCCATCCCCGGACTC--------------------------------------C | 38 bp deleted → frameshift |
| 74 | CGGCTCTCCATCCCCGGACTCTCCAGAG----------------------------CTCC | 28 bp deleted → frameshift |
| 73 | CGG----------------------------------GGGAAAACCCCCAAGTAAGCTCC | 34 bp deleted → frameshift |
| 69 | CGG-----------------------------------GGAAAACCCCCAAGTAAGCTCC | 35 bp deleted → frameshift |
| 67 | CGGCTCTCCA----------------------------------------AGTAAGCTCC | 40 bp deleted → frameshift |
| 66 | CGGCTCTCCATCCCCGGACTCTCCAG------------------------------CTCC | 30 bp deleted → no frameshift |
| 66 | CGGCTCTCCATCC------------------------------------AAGTAAGCTCC | 36 bp deleted → no frameshift |
| 66 | CGG------------------------------------GAAAACCCCCAAGTAAGCTCC | 36 bp deleted → no frameshift |
| 62 | CGGCTCTCC-------------------------------------CCCAAGTAAGCTCC | 37 bp deleted → frameshift |
| 62 | CGG-------------------------------------AAAACCCCCAAGTAAGCTCC | 37 bp deleted → frameshift |
| 60 | CGGCTCTCC--------------------------------------CCAAGTAAGCTCC | 38 bp deleted → frameshift |
| 56 | CGGCTCTCC---------------------------------------CAAGTAAGCTCC | 39 bp deleted → no frameshift |
| 54 | CGGCTCTCC--------------------------------------------------- | 51 bp deleted → no frameshift |
| 49 | CGGCTC-----------------------------------------------------C | 53 bp deleted → frameshift |
| 47 | CGGCTCTCCATCC----------------------------------------------- | 47 bp deleted → frameshift |
| 42 | CGGCTCTCCATCCCC--------------------------------------------- | 45 bp deleted → no frameshift |
| 40 | CGGCTCTCCATCCC---------------------------------------------- | 46 bp deleted → frameshift |