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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313879_F | Primer3: not found at this Tm | N.d. | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313879_R | Primer3: not found at this Tm | N.d. | 1.00 |
| mm3_intron_Lpp_chr16_24666195_F | TCGTCGGCAGCGTCTGCTTGGTAGGCTTCCCAAG | 59.9 | 0.77 |
| mm3_intron_Lpp_chr16_24666195_R | GTCTCGTGGGCTCGGACCACATGCAAGGCCTAGTC | 60.0 | 0.77 |
| mm4_intron_Ttc24_chr3_88072924_F | TCGTCGGCAGCGTCCCACAGACCCTTCAGGCATT | 59.9 | 0.65 |
| mm4_intron_Ttc24_chr3_88072924_R | GTCTCGTGGGCTCGGGCTGCCCAAGACACTGGTAA | 60.2 | 0.65 |
| mm4_intron_Nfkb1_chr3_135600062_F | TCGTCGGCAGCGTCCTGGAAGCAGAGGTCTCAGC | 60.1 | 0.56 |
| mm4_intron_Nfkb1_chr3_135600062_R | GTCTCGTGGGCTCGGAACCCTTTTGTGCCCCTACC | 60.1 | 0.56 |
| mm2_intergenic_1700029M20Rik|Ifnlr1_chr4_135638918_F | Primer3: not found at this Tm | N.d. | 0.55 |
| mm2_intergenic_1700029M20Rik|Ifnlr1_chr4_135638918_R | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intergenic_Atxn7l1|Gm11052_chr12_33151544_F | TCGTCGGCAGCGTCCTTGTGAGGGCTGCCTTAGG | 60.3 | 0.52 |
| mm4_intergenic_Atxn7l1|Gm11052_chr12_33151544_R | GTCTCGTGGGCTCGGTCAACCCAGAGAAGTGAGCC | 59.3 | 0.52 |
| mm4_intron_Cacnb2_chr2_14893081_F | TCGTCGGCAGCGTCATCCTTACCCAACCTCAGGT | 57.6 | 0.46 |
| mm4_intron_Cacnb2_chr2_14893081_R | GTCTCGTGGGCTCGGAGAAGGATCACAACGGTGGG | 59.6 | 0.46 |
| mm4_intergenic_Mppe1|Impa2_chr18_67249296_F | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4_intergenic_Mppe1|Impa2_chr18_67249296_R | Primer3: not found at this Tm | N.d. | 0.39 |
| mm3_intergenic_Dgkb|Etv1_chr12_38716212_F | Primer3: not found at this Tm | N.d. | 0.38 |
| mm3_intergenic_Dgkb|Etv1_chr12_38716212_R | Primer3: not found at this Tm | N.d. | 0.38 |
| mm4_intergenic_Notch3|Ephx3_chr17_32180464_F | TCGTCGGCAGCGTCGCCTTCCATTCCCTCTTGCT | 60.0 | 0.35 |
| mm4_intergenic_Notch3|Ephx3_chr17_32180464_R | GTCTCGTGGGCTCGGTATGGCTGTTGCTCTCTGGC | 60.1 | 0.35 |
| mm4_intergenic_Sox15|Sox15/Mpdu1_chr11_69655980_F | TCGTCGGCAGCGTCCTTCCTTGAGAGCTGGGTGG | 60.0 | 0.34 |
| mm4_intergenic_Sox15|Sox15/Mpdu1_chr11_69655980_R | GTCTCGTGGGCTCGGCCTCAGCCTGGTGTAGCTTC | 60.1 | 0.34 |
| mm4_intergenic_Nav1|Nav1/Gm4793_chr1_135561759_F | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_intergenic_Nav1|Nav1/Gm4793_chr1_135561759_R | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_intron_Dach2_chrX_113515557_F | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_intron_Dach2_chrX_113515557_R | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_intergenic_Nphp4|Gm13172_chr4_152607175_F | TCGTCGGCAGCGTCGGGCTCAGTGTGGTATCAGG | 59.8 | 0.33 |
| mm4_intergenic_Nphp4|Gm13172_chr4_152607175_R | GTCTCGTGGGCTCGGGCTACCCATCTGAGCCAGTC | 59.8 | 0.33 |
| mm4_intergenic_Prdm6|Cep120_chr18_53628372_F | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_intergenic_Prdm6|Cep120_chr18_53628372_R | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_intron_Ptpn1_chr2_167950880_F | TCGTCGGCAGCGTCGGCACAGGCTCAGAATGGTA | 59.7 | 0.31 |
| mm4_intron_Ptpn1_chr2_167950880_R | GTCTCGTGGGCTCGGCTTCCGGCAGTGTCTACAGG | 60.1 | 0.31 |
| mm3_intergenic_Gm25011|Gm23680_chr10_12083689_F | TCGTCGGCAGCGTCGCGATGAAAATTCCCTGCGT | 59.5 | 0.29 |
| mm3_intergenic_Gm25011|Gm23680_chr10_12083689_R | GTCTCGTGGGCTCGGGTGATAGCACTGCAAACGGG | 59.5 | 0.29 |
| mm4_intron_D430042O09Rik_chr7_125768115_F | TCGTCGGCAGCGTCCACATTGTCCAGGGACCACA | 59.8 | 0.29 |
| mm4_intron_D430042O09Rik_chr7_125768115_R | GTCTCGTGGGCTCGGTGGGAGCAGGAGAGATGTGA | 59.9 | 0.29 |
| mm4_intergenic_Gm14373|Gm14452_chrX_5806626_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intergenic_Gm14373|Gm14452_chrX_5806626_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intergenic_4930564K09Rik|Lrat_chr3_82886990_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_4930564K09Rik|Lrat_chr3_82886990_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intron_Otub2_chr12_103396312_F | TCGTCGGCAGCGTCGAGGACTTGGGCCATTCCAA | 59.9 | 0.27 |
| mm4_intron_Otub2_chr12_103396312_R | GTCTCGTGGGCTCGGGAGAGAGGGAGGGAGGAAGG | 60.1 | 0.27 |
| mm4_intergenic_Tmem26|Gm24172_chr10_68836134_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Tmem26|Gm24172_chr10_68836134_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Garem_chr18_21201846_F | TCGTCGGCAGCGTCACTTCCAGGAGTCAGGAATGG | 59.0 | 0.24 |
| mm4_intron_Garem_chr18_21201846_R | GTCTCGTGGGCTCGGTCTCCTGTGCTTGTTAGACTTCA | 59.3 | 0.24 |
| mm4_intron_Snd1_chr6_28516789_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Snd1_chr6_28516789_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm3_intron_Ext1_chr15_53331438_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm3_intron_Ext1_chr15_53331438_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Tmem61|2700068H02Rik_chr4_106537459_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Tmem61|2700068H02Rik_chr4_106537459_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm3_intron_Plcb1_chr2_134903516_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm3_intron_Plcb1_chr2_134903516_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Ankrd22|Stambpl1_chr19_34169106_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Ankrd22|Stambpl1_chr19_34169106_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Gm12149|Gm12152_chr11_43653354_F | TCGTCGGCAGCGTCACTGCAAATCCCTCGGAGTC | 59.7 | 0.19 |
| mm4_intergenic_Gm12149|Gm12152_chr11_43653354_R | GTCTCGTGGGCTCGGCATCTGCCCAGTTTGTGCAC | 60.0 | 0.19 |
| mm3_intergenic_Gm23466|Gm12351_chr4_34455906_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm3_intergenic_Gm23466|Gm12351_chr4_34455906_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_Csmd3_chr15_48076610_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Csmd3_chr15_48076610_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Ppapdc1a_chr7_129319015_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Ppapdc1a_chr7_129319015_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm23163|Pmfbp1_chr8_109491894_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm23163|Pmfbp1_chr8_109491894_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3_exon_Pard3_chr8_127610503_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3_exon_Pard3_chr8_127610503_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3_intergenic_Slitrk1|Gm25901_chr14_109705877_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm3_intergenic_Slitrk1|Gm25901_chr14_109705877_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Ctnnbl1_chr2_157878813_F | TCGTCGGCAGCGTCACCTCTTGGGGTTCAGAGGA | 59.8 | 0.14 |
| mm4_intron_Ctnnbl1_chr2_157878813_R | GTCTCGTGGGCTCGGGTGAGTCCAGCATCCTTCCC | 60.1 | 0.14 |
| mm3_intergenic_U6|Mylk4_chr13_32762055_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm3_intergenic_U6|Mylk4_chr13_32762055_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm23888|Cd209g_chr8_4124316_F | TCGTCGGCAGCGTCCCAGCTGGGCTTGTTGACTA | 59.9 | 0.13 |
| mm4_intergenic_Gm23888|Cd209g_chr8_4124316_R | GTCTCGTGGGCTCGGTCTCTCCTTCAAGTCACTTGCA | 59.3 | 0.13 |
| mm4_intron_Efnb1_chrX_99140007_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Efnb1_chrX_99140007_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Shroom2_chrX_152759419_F | TCGTCGGCAGCGTCTGCACTGCAGAAAACGGAGA | 60.1 | 0.12 |
| mm4_intron_Shroom2_chrX_152759419_R | GTCTCGTGGGCTCGGATTCTCAGTGGTGGCACATC | 57.8 | 0.12 |
| mm4_intron_Hdac4_chr1_92072392_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Hdac4_chr1_92072392_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Mars|Gm6283_chr10_127311822_F | TCGTCGGCAGCGTCCAACGAGACCCGGAAGTTGA | 59.9 | 0.11 |
| mm4_intergenic_Mars|Gm6283_chr10_127311822_R | GTCTCGTGGGCTCGGCAGAAAATGCCACCCATCGC | 60.1 | 0.11 |
| mm4_intergenic_Vapa|Txndc2_chr17_65629049_F | TCGTCGGCAGCGTCATGGTTCAGAGCAGGGTTGG | 59.9 | 0.11 |
| mm4_intergenic_Vapa|Txndc2_chr17_65629049_R | GTCTCGTGGGCTCGGTGTTATGGAGCCCAGACTGC | 59.7 | 0.11 |
| mm4_intron_Pphln1_chr15_93483212_F | TCGTCGGCAGCGTCTATGGTGTCCGTGCCTCTCT | 60.3 | 0.10 |
| mm4_intron_Pphln1_chr15_93483212_R | GTCTCGTGGGCTCGGGTGAAGAGTGGTGGGGACTG | 59.9 | 0.10 |
| mm4_intergenic_Gm15891/Trim42|Gm25607_chr9_97417623_F | TCGTCGGCAGCGTCTGCAAGTCGGATAATGAGCT | 57.0 | 0.10 |
| mm4_intergenic_Gm15891/Trim42|Gm25607_chr9_97417623_R | GTCTCGTGGGCTCGGCTGGGTTCCACCTGTCTTCC | 59.9 | 0.10 |
| mm4_intron_Gucy1a3_chr3_82110913_F | TCGTCGGCAGCGTCTGAAACCAAAGTCTGTGACTGT | 58.0 | 0.10 |
| mm4_intron_Gucy1a3_chr3_82110913_R | GTCTCGTGGGCTCGGGGCCTCAAGCATTCCTCCTT | 60.0 | 0.10 |
| mm4_intergenic_Gm12983|Gm12984_chr4_135303203_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm12983|Gm12984_chr4_135303203_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Arpp21_chr9_112075448_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Arpp21_chr9_112075448_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm2_intergenic_Gm10192|Gm12696_chr4_97488878_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm2_intergenic_Gm10192|Gm12696_chr4_97488878_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Fuca1|Hmgcl_chr4_135945254_F | TCGTCGGCAGCGTCTACAAAAGCAGGCCAGAGCA | 59.8 | 0.09 |
| mm4_intergenic_Fuca1|Hmgcl_chr4_135945254_R | GTCTCGTGGGCTCGGAATCCTGACCTGACCTTGGG | 59.0 | 0.09 |
| mm4_intron_Syt9_chr7_107371588_F | TCGTCGGCAGCGTCATTTCCACCCGAAGCTGCTC | 60.6 | 0.09 |
| mm4_intron_Syt9_chr7_107371588_R | GTCTCGTGGGCTCGGGAAAGGAGAGGGTTCAGGCC | 60.0 | 0.09 |
| mm4_intron_Panx3_chr9_37665969_F | TCGTCGGCAGCGTCCCAGAAAGACTCAGGAACTAGGG | 59.8 | 0.09 |
| mm4_intron_Panx3_chr9_37665969_R | GTCTCGTGGGCTCGGAGGATGGTTGGTTAAATGCTGT | 58.2 | 0.09 |
| mm4_intergenic_Galnt1|Mir187_chr18_24288392_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Galnt1|Mir187_chr18_24288392_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_E130006D01Rik|Miat/MIAT_exon5_3/MIAT_exon5_2/Gm26953/MIAT_exon5_1_chr5_111845482_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_E130006D01Rik|Miat/MIAT_exon5_3/MIAT_exon5_2/Gm26953/MIAT_exon5_1_chr5_111845482_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Mrp63_chr14_57826428_F | TCGTCGGCAGCGTCCACTCACTTCCGGCGAGAG | 60.1 | 0.08 |
| mm4_intron_Mrp63_chr14_57826428_R | GTCTCGTGGGCTCGGGGAAATGACACGAAAGCCGC | 60.4 | 0.08 |
| mm4_intron_Osbpl5_chr7_143746470_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Osbpl5_chr7_143746470_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_5830416P10Rik|Dusp5_chr19_53511666_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_5830416P10Rik|Dusp5_chr19_53511666_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intergenic_Gpr180|Sox21/Gm4675_chr14_118211528_F | TCGTCGGCAGCGTCCTGCTGTTTCCCCATGCATG | 59.8 | 0.08 |
| mm3_intergenic_Gpr180|Sox21/Gm4675_chr14_118211528_R | GTCTCGTGGGCTCGGACAGGCATGGCATTTTGCTG | 60.0 | 0.08 |
| mm4_intergenic_Il24|Il20_chr1_130906692_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Il24|Il20_chr1_130906692_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intron_Tpd52l1_chr10_31342473_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intron_Tpd52l1_chr10_31342473_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_AU040320_chr4_126754159_F | TCGTCGGCAGCGTCCTGCTTCTAGCCTTGCAGGT | 60.0 | 0.07 |
| mm4_intron_AU040320_chr4_126754159_R | GTCTCGTGGGCTCGGGGTCTGCCCATATGTCAGGG | 59.8 | 0.07 |
| mm4_intergenic_Ints10|Lpl_chr8_68870577_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Ints10|Lpl_chr8_68870577_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Csrp1_chr1_135735623_F | TCGTCGGCAGCGTCCTGGTCTGAGGCTTCCTTCG | 60.1 | 0.07 |
| mm4_intron_Csrp1_chr1_135735623_R | GTCTCGTGGGCTCGGCCTCAGCTTGCTTGACCTCA | 59.9 | 0.07 |
| mm4_intergenic_Epha6|Gm24755_chr16_60240357_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Epha6|Gm24755_chr16_60240357_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm23529|Ferd3l_chr12_33672534_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm23529|Ferd3l_chr12_33672534_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm17566|Gm23153_chr1_190333410_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm17566|Gm23153_chr1_190333410_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_n-R5s188|Nfib_chr4_82455684_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_n-R5s188|Nfib_chr4_82455684_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Trio_chr15_27854722_F | TCGTCGGCAGCGTCTGCCCCTGGTACTCCATACA | 59.9 | 0.06 |
| mm4_intron_Trio_chr15_27854722_R | GTCTCGTGGGCTCGGACACTTCCTTGGCCATTGGG | 60.5 | 0.06 |
| mm4_intergenic_Gm10660|Nat1_chr8_67437859_F | TCGTCGGCAGCGTCACCATGAACTTCCCGACACC | 59.9 | 0.06 |
| mm4_intergenic_Gm10660|Nat1_chr8_67437859_R | GTCTCGTGGGCTCGGTGTTGCTATGGTGAGACTGTGA | 59.3 | 0.06 |
| mm4_intergenic_n-R5s30|Rbfox1_chr16_5625300_F | TCGTCGGCAGCGTCTTGGAAAAGCAGAGCCCACA | 60.1 | 0.06 |
| mm4_intergenic_n-R5s30|Rbfox1_chr16_5625300_R | GTCTCGTGGGCTCGGAAGCCAAGAGAGCTCTGCAC | 60.3 | 0.06 |
| mm4_intron_Os9_chr10_127097726_F | TCGTCGGCAGCGTCGCACAGAGCCCCTTCTTCAT | 60.0 | 0.05 |
| mm4_intron_Os9_chr10_127097726_R | GTCTCGTGGGCTCGGCCTGGTTTGCTCTATGCCCA | 60.0 | 0.05 |
| mm4_intron_Htr7_chr19_36014781_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Htr7_chr19_36014781_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Rab40b|Gm11866_chr11_121410813_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Rab40b|Gm11866_chr11_121410813_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Lepre1_chr4_119238862_F | TCGTCGGCAGCGTCAGACTGGCTTCTGGCTGAAC | 59.9 | 0.05 |
| mm4_intron_Lepre1_chr4_119238862_R | GTCTCGTGGGCTCGGACTCCTGGTACTCCTTCCCA | 59.2 | 0.05 |
| mm4_exon_Lefty1_chr1_180935119_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Lefty1_chr1_180935119_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Ldb3_chr14_34564868_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Ldb3_chr14_34564868_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Ppm1l_chr3_69317651_F | TCGTCGGCAGCGTCCTGAGACCCGAGACGCTTTT | 60.0 | 0.05 |
| mm4_exon_Ppm1l_chr3_69317651_R | GTCTCGTGGGCTCGGCTTCACTTCGTCCGTGTGGA | 59.9 | 0.05 |
| mm4_intergenic_Sox5|Sox5/4933415D12Rik_chr6_143963142_F | TCGTCGGCAGCGTCACCTGACACCGTATTACTCACT | 58.5 | 0.05 |
| mm4_intergenic_Sox5|Sox5/4933415D12Rik_chr6_143963142_R | GTCTCGTGGGCTCGGAATCTTGGTTTTCTTGGTGATCAT | 57.0 | 0.05 |
| mm4_exon_2610204G22Rik_chr4_155761916_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_2610204G22Rik_chr4_155761916_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Gm3468_chr14_6108200_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Gm3468_chr14_6108200_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Gm2956_chr14_3333062_F | TCGTCGGCAGCGTCGGGGTTCACCTCTTGACCAT | 59.3 | 0.04 |
| mm4_intron_Gm2956_chr14_3333062_R | GTCTCGTGGGCTCGGGAACAGTTCAGGGCAGCCTA | 59.6 | 0.04 |
| mm4_intron_Gm2237_chr14_19584683_F | TCGTCGGCAGCGTCGAACAGTTCAGGGCAGCCTA | 59.6 | 0.04 |
| mm4_intron_Gm2237_chr14_19584683_R | GTCTCGTGGGCTCGGGGGGTTCACCTCTTGACCAT | 59.3 | 0.04 |
| mm4_intergenic_Gm26680|Gm8362_chr14_6756572_F | TCGTCGGCAGCGTCGAACAGTTCAGGGCAGCCTA | 59.6 | 0.04 |
| mm4_intergenic_Gm26680|Gm8362_chr14_6756572_R | GTCTCGTGGGCTCGGGGGGTTCACCTCTTGACCAT | 59.3 | 0.04 |
| mm4_intron_Grik5_chr7_25050091_F | TCGTCGGCAGCGTCGCACAGAGCTGAGATCCACA | 59.7 | 0.04 |
| mm4_intron_Grik5_chr7_25050091_R | GTCTCGTGGGCTCGGAAGCTCAGACTGCTGTGGAC | 59.9 | 0.04 |
| mm4_intergenic_Uqcrc2/Pdzd9|Pdzd9_chr7_120662281_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Uqcrc2/Pdzd9|Pdzd9_chr7_120662281_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Tns3_chr11_8470937_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Tns3_chr11_8470937_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Gpr125_chr5_50049123_F | TCGTCGGCAGCGTCAAGTGCACAGATAAGGCGCT | 60.0 | 0.04 |
| mm4_intron_Gpr125_chr5_50049123_R | GTCTCGTGGGCTCGGGGAGATTGCGAGCACAAAGC | 60.1 | 0.04 |
| mm4_intergenic_Gm12708|Gm830_chr4_95290783_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm12708|Gm830_chr4_95290783_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm26338|Tspan9_chr6_128122583_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm26338|Tspan9_chr6_128122583_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Onecut1|Onecut1/Gm20649_chr9_74870611_F | TCGTCGGCAGCGTCGGCAAGGCCCATCTCATTAGA | 59.8 | 0.04 |
| mm4_intergenic_Onecut1|Onecut1/Gm20649_chr9_74870611_R | GTCTCGTGGGCTCGGAGAACGTCAGCCTTGGAAGC | 60.6 | 0.04 |
| mm4_intergenic_Gm4419|Gm5633_chr12_21658081_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm4419|Gm5633_chr12_21658081_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Pou3f4|Cylc1_chrX_110851350_F | TCGTCGGCAGCGTCAAGTGAAAACCCACTCGGCT | 59.8 | 0.03 |
| mm4_intergenic_Pou3f4|Cylc1_chrX_110851350_R | GTCTCGTGGGCTCGGCCGCTGTTAGCCTCTCATGT | 59.8 | 0.03 |
| mm4_intergenic_Itprip|Itprip/Wdr96/Gm22240_chr19_47903141_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Itprip|Itprip/Wdr96/Gm22240_chr19_47903141_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Arhgap26|Fgf1_chr18_38804352_F | TCGTCGGCAGCGTCGAATCGCTTCCTGCAGCATG | 59.9 | 0.03 |
| mm4_intergenic_Arhgap26|Fgf1_chr18_38804352_R | GTCTCGTGGGCTCGGCAGGTGTTTGTCCCTCCACA | 59.8 | 0.03 |
| mm4_intergenic_Phyhipl|4930533K18Rik_chr10_70742257_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Phyhipl|4930533K18Rik_chr10_70742257_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Aim1l_chr4_134081174_F | TCGTCGGCAGCGTCGCAGAGCTGGGAAGTAAGCA | 60.0 | 0.03 |
| mm4_exon_Aim1l_chr4_134081174_R | GTCTCGTGGGCTCGGCTTCAATCCTCACCAGCCCC | 60.3 | 0.03 |
| mm4_intergenic_Gm25383|Gm24863_chr13_79320322_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm25383|Gm24863_chr13_79320322_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm15219|Cdkn3_chr14_46626531_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm15219|Cdkn3_chr14_46626531_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_exon_Slc9a1_chr4_133417933_F | TCGTCGGCAGCGTCTCCTGACCTGGTTCATCAACA | 58.9 | 0.03 |
| mm3_exon_Slc9a1_chr4_133417933_R | GTCTCGTGGGCTCGGGGGGAAGTGCTTCTTGTCCA | 59.8 | 0.03 |
| mm4_intron_Usp48_chr4_137601301_F | TCGTCGGCAGCGTCTGGGCATGACTTCCTTGTGT | 59.5 | 0.02 |
| mm4_intron_Usp48_chr4_137601301_R | GTCTCGTGGGCTCGGGGCAGTCCTACGGGTCTTTC | 60.1 | 0.02 |
| mm4_intergenic_Plekhb1|Fam168a_chr7_100686662_F | TCGTCGGCAGCGTCCTCCACCTATCAGAACAGTGCA | 59.7 | 0.02 |
| mm4_intergenic_Plekhb1|Fam168a_chr7_100686662_R | GTCTCGTGGGCTCGGCCTCAGGCAACCTCAGGATG | 60.1 | 0.02 |
| mm4_intron_Sytl3_chr17_6734806_F | TCGTCGGCAGCGTCAGCAGAAGGACAAGGCCTTC | 59.9 | 0.02 |
| mm4_intron_Sytl3_chr17_6734806_R | GTCTCGTGGGCTCGGCCCAGAAGCCCTCTTGGTTC | 60.3 | 0.02 |
| mm4_intron_Samd12_chr15_53697548_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Samd12_chr15_53697548_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Nkd2|Trip13_chr13_73888644_F | TCGTCGGCAGCGTCGAGTGCAGTCAGGACCCAAA | 59.8 | 0.02 |
| mm4_intergenic_Nkd2|Trip13_chr13_73888644_R | GTCTCGTGGGCTCGGCCTTCAATGGCAGCTCCTGA | 60.0 | 0.02 |
| mm4_intron_Cacna2d3_chr14_29638505_F | TCGTCGGCAGCGTCCTTACACTTCCTCCCTGCCC | 59.7 | 0.02 |
| mm4_intron_Cacna2d3_chr14_29638505_R | GTCTCGTGGGCTCGGACATTTCCTGCTGATAAAGGGGA | 59.7 | 0.02 |
| mm4_intergenic_Ube2cbp|Dopey1_chr9_86465039_F | TCGTCGGCAGCGTCGCTCTAGTAGGACAGGTGCG | 59.6 | 0.02 |
| mm4_intergenic_Ube2cbp|Dopey1_chr9_86465039_R | GTCTCGTGGGCTCGGGCCCATCCAACATCCTTCCA | 60.0 | 0.02 |
| mm4_intron_Camta1_chr4_151539688_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Camta1_chr4_151539688_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm6564|AC158942.1_chr5_22977098_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm6564|AC158942.1_chr5_22977098_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Gm26815_chr8_121330403_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Gm26815_chr8_121330403_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Ap3s2_chr7_79902466_F | TCGTCGGCAGCGTCAAGATGTGTGTAGGCCTGCC | 60.0 | 0.02 |
| mm4_intron_Ap3s2_chr7_79902466_R | GTCTCGTGGGCTCGGTAGTGCAGGACAGGAACCCA | 60.4 | 0.02 |
| mm4_exon_C77080_chr4_129222083_F | TCGTCGGCAGCGTCAGTTCAGCCACCAGATTCCG | 60.0 | 0.02 |
| mm4_exon_C77080_chr4_129222083_R | GTCTCGTGGGCTCGGCACAGTCTCCTGCCTCCAAG | 60.0 | 0.02 |
| mm4_intergenic_Gm26861|Gm25496_chr13_11219462_F | TCGTCGGCAGCGTCGCTCTGCTGCCTCATGGAAT | 60.4 | 0.02 |
| mm4_intergenic_Gm26861|Gm25496_chr13_11219462_R | GTCTCGTGGGCTCGGGCAGCCTTACCCAGCCTAAT | 59.8 | 0.02 |
| mm4_intergenic_1700009N14Rik|Gm12385_chr4_39577194_F | TCGTCGGCAGCGTCTGATACTCAGATATGGGCATTTGC | 58.9 | 0.02 |
| mm4_intergenic_1700009N14Rik|Gm12385_chr4_39577194_R | GTCTCGTGGGCTCGGTCTGTGCTATGATCTGTGTTGCT | 60.0 | 0.02 |
| mm4_intergenic_Cacng1|Cacng4_chr11_107724217_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Cacng1|Cacng4_chr11_107724217_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Gm10619_chr7_73815807_F | TCGTCGGCAGCGTCCCCAAAGGTTTGAGACGACAC | 59.3 | 0.02 |
| mm4_intron_Gm10619_chr7_73815807_R | GTCTCGTGGGCTCGGAGGATGCCAAACCATTGACTG | 58.8 | 0.02 |
| mm4_intergenic_Gm25115|Diap3_chr14_86440731_F | TCGTCGGCAGCGTCTGGTACTGTTTCAGCGGAGAC | 59.9 | 0.01 |
| mm4_intergenic_Gm25115|Diap3_chr14_86440731_R | GTCTCGTGGGCTCGGTGCTGAGTTTCCTCAAAGGGA | 59.2 | 0.01 |
| mm4_intergenic_Gm24129|Tecrl_chr5_83098091_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm24129|Tecrl_chr5_83098091_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm23965|Gm23537_chr1_104468722_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm23965|Gm23537_chr1_104468722_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Psma6|Nfkbia_chr12_55439130_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Psma6|Nfkbia_chr12_55439130_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Smad1|Gm8079_chr8_79420788_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Smad1|Gm8079_chr8_79420788_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Asic2|4930527B05Rik_chr11_81322083_F | TCGTCGGCAGCGTCTCAGTGAGCATGATCTCTGTGT | 59.1 | 0.01 |
| mm4_intergenic_Asic2|4930527B05Rik_chr11_81322083_R | GTCTCGTGGGCTCGGTGTCCAAGCAGCCATCAGAG | 60.0 | 0.01 |
| mm4_intron_Eef2k_chr7_120902554_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Eef2k_chr7_120902554_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Grb10_chr11_11999875_F | TCGTCGGCAGCGTCAACCTACTTATTTGTCTAGTGAGGT | 57.2 | 0.01 |
| mm4_intron_Grb10_chr11_11999875_R | GTCTCGTGGGCTCGGACTCAGCATCAAGATGACCACA | 59.6 | 0.01 |
| mm4_intron_Slc26a9_chr1_131765051_F | TCGTCGGCAGCGTCTCCTCTAGCTAAGCTCCACCT | 59.4 | 0.01 |
| mm4_intron_Slc26a9_chr1_131765051_R | GTCTCGTGGGCTCGGGTCCCCACTGCATCCCTTTC | 60.6 | 0.01 |
| mm4_intron_Aox4_chr1_58224703_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Aox4_chr1_58224703_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Csmd2_chr4_128557885_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Csmd2_chr4_128557885_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Esr1_chr10_4817693_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Esr1_chr10_4817693_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm7108|4930415L06Rik_chrX_89890682_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm7108|4930415L06Rik_chrX_89890682_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm13903|Kcna4_chr2_107157356_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm13903|Kcna4_chr2_107157356_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Rab8a_chr8_72169574_F | TCGTCGGCAGCGTCGTGTGTGGACCTGAAACCCA | 60.1 | 0.01 |
| mm4_intron_Rab8a_chr8_72169574_R | GTCTCGTGGGCTCGGTGGGGTCCTAGATGCTCACA | 59.9 | 0.01 |
| mm4_intergenic_Cacna1e|Gm9530_chr1_154954192_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Cacna1e|Gm9530_chr1_154954192_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Aqp3_chr4_41097852_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Aqp3_chr4_41097852_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm13810|B230118H07Rik_chr2_100945990_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm13810|B230118H07Rik_chr2_100945990_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Kmt2d_chr15_98844109_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Kmt2d_chr15_98844109_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm24691|Grm8_chr6_26641028_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm24691|Grm8_chr6_26641028_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Pmfbp1_chr8_109537948_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Pmfbp1_chr8_109537948_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm15202|Gm26317_chrX_161973950_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm15202|Gm26317_chrX_161973950_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_1700060C16Rik_chr6_143635139_F | TCGTCGGCAGCGTCTCTCTCCCCATTCCTCCAGG | 60.0 | 0.00 |
| mm4_intron_1700060C16Rik_chr6_143635139_R | GTCTCGTGGGCTCGGTAGCCCTCGACACTTAGCCT | 60.0 | 0.00 |
| mm3_intron_Ildr1_chr16_36706754_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intron_Ildr1_chr16_36706754_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Osbpl11_chr16_33241481_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Osbpl11_chr16_33241481_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Tssc1|Gm23342_chr12_28988016_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Tssc1|Gm23342_chr12_28988016_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm2_intron_Sipa1l2_chr8_125442883_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm2_intron_Sipa1l2_chr8_125442883_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Thsd7b_chr1_129309960_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Thsd7b_chr1_129309960_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Haus8/Myo9b|Myo9b_chr8_71274592_F | TCGTCGGCAGCGTCATGCTCTGGCTTTGACCTCC | 60.0 | 0.00 |
| mm4_intergenic_Haus8/Myo9b|Myo9b_chr8_71274592_R | GTCTCGTGGGCTCGGGTCAGGAAGGGAGTCAAGCC | 60.0 | 0.00 |
| mm4_intron_Nxph3_chr11_95511686_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Nxph3_chr11_95511686_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Mllt6_chr11_97666693_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Mllt6_chr11_97666693_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Gabre_chrX_72270535_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Gabre_chrX_72270535_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_2410004I01Rik|Dcakd_chr11_102982259_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_2410004I01Rik|Dcakd_chr11_102982259_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Leo1_chr9_75456602_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Leo1_chr9_75456602_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Rorc_chr3_94388400_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Rorc_chr3_94388400_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Muc5ac_chr7_141795801_F | TCGTCGGCAGCGTCACCAAGGTTCAGTGAGGGAG | 58.9 | 0.00 |
| mm4_intron_Muc5ac_chr7_141795801_R | GTCTCGTGGGCTCGGCACTGGCAACTGGGTGTAGA | 59.6 | 0.00 |
| mm4_intron_Cog7_chr7_121936445_F | TCGTCGGCAGCGTCGCAAATGCAGCAGCTAGTGG | 60.1 | 0.00 |
| mm4_intron_Cog7_chr7_121936445_R | GTCTCGTGGGCTCGGGCTCTTCTGATGGGTGCAGT | 60.0 | 0.00 |
| mm4_intergenic_Card10|Cdc42ep1_chr15_78827205_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Card10|Cdc42ep1_chr15_78827205_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Pkdrej|Ttc38_chr15_85831516_F | TCGTCGGCAGCGTCTGAATTTTCCAGCCTCTCCAC | 58.2 | 0.00 |
| mm4_intergenic_Pkdrej|Ttc38_chr15_85831516_R | GTCTCGTGGGCTCGGACAGTCTCCCTCTCCCCTTC | 59.9 | 0.00 |
| mm4_intergenic_A930011G23Rik|Gm16227_chr5_99416153_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_A930011G23Rik|Gm16227_chr5_99416153_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ppargc1a|mmu-mir-6417_chr5_52129792_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ppargc1a|mmu-mir-6417_chr5_52129792_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| mm3_intron_Lpp_chr16_24666195 |
TGCTTGGTAGGCTTCCCAAGCATCTGGGGGACAACCTGGCACTCTGGATGACACTGGCTTGATCTTTTCATGACCTCCAG TAAAGACCTTTGACTAGGCCTTGCATGTGGT |
| mm4_intron_Ttc24_chr3_88072924 |
CCACAGACCCTTCAGGCATTGTGTACCCTCGGTCTTTGTGTCCCCCTCCCTGAGTTCCAAGCTAACCCTCTCATTCTTAC CAGTGTCTTGGGCAGC |
| mm4_intron_Nfkb1_chr3_135600062 |
CTGGAAGCAGAGGTCTCAGCATGGGCCTGGCTGTGGGGATCAGAATGACACTCTGGGCAAGAGAGTGCAGAGGAACTTAG AAGCCAACAAGAGGATAAGACCAACAGGCCCAGAGAGAGGGGTAGGGGCACAAAAGGGTT |
| mm4_intergenic_Atxn7l1|Gm11052_chr12_33151544 |
CTTGTGAGGGCTGCCTTAGGGTAGCTTCACTGAAAGACGAACTAGGGAGAACTGGGAGCCAACCTGGCACTCAGGCCCTG GGGAGAAAAAGGCTCACTTCTCTGGGTTGA |
| mm4_intron_Cacnb2_chr2_14893081 |
ATCCTTACCCAACCTCAGGTTAAGCTGAGGTCAGCTTGACACTCAGGGACATAGTACTTATGAGAACCATAACTCACCTG CCCACCGTTGTGATCCTTCT |
| mm4_intergenic_Notch3|Ephx3_chr17_32180464 |
GCCTTCCATTCCCTCTTGCTCTGTGAGCTCCAGAATTCCAAGATGGCCCCCAGAACCTACAAAAATGGCTGATATTGGGA TGCTGAGTAAAAAGCATGTCTCTTTAGGAACAAAGAGGCCAGAGAGCAACAGCCATA |
| mm4_intergenic_Sox15|Sox15/Mpdu1_chr11_69655980 |
CTTCCTTGAGAGCTGGGTGGGAACAGAGGCCAGAGTGCCAAGTTAAACCCAAATCCACCCAAGCAGAAGCTACACCAGGC TGAGG |
| mm4_intergenic_Nphp4|Gm13172_chr4_152607175 |
GGGCTCAGTGTGGTATCAGGAGTCTGAGGGCTGGGGTGAGCCTGGCACTCAGGGTCTGCAGCACTGTGGGGTAAGACTGG CTCAGATGGGTAGC |
| mm4_intron_Ptpn1_chr2_167950880 |
GGCACAGGCTCAGAATGGTAACCATGCGTGACAGTACAGACAGTTCTCCGTGGATGTGCCACACCTCAGGAGCTCGGAGT TTGCTTGGCACTCAGGTTGTGTTTTCCTGTAGACACTGCCGGAAG |
| mm3_intergenic_Gm25011|Gm23680_chr10_12083689 |
GCGATGAAAATTCCCTGCGTCAGGGATCCGTTCTATTCATGTAGGTGCTGTACTGCTCACAGCTATGGCCTGATTGCCAT GCTGAGCCCCATGGGAATAGATACATGTTGCTTCAGAGCCCCGTTTGCAGTGCTATCAC |
| mm4_intron_D430042O09Rik_chr7_125768115 |
CACATTGTCCAGGGACCACAGGCCTCAATCCCTGCCTCTCAACTGGTCTTTTCCTGTGCCCCAGAATGCCAAGATAACCC CAACTCCTCACTGTTCTGTTTCCCAATTTCACATCTCTCCTGCTCCCA |
| mm4_intron_Otub2_chr12_103396312 |
GAGGACTTGGGCCATTCCAAAGGTGTTTTAGAGACTTCCAGTGCCAGGGTGGAGATCTGCTTGGCACTAGGGTCACATAT ACACTTGGGGGACACTAGATGTCAACCCAAGACCCATCTACCTTCCTCCCTCCCTCTCTC |
| mm4_intron_Garem_chr18_21201846 |
ACTTCCAGGAGTCAGGAATGGAAGATAAAATGGTCAGGGCATCAGCTTGGTACTCAGGTTTCCTTGCTTCACATTCGTAT TTCAGTTCCTAACAATACGGTGAAATGAAGTCTAACAAGCACAGGAGA |
| mm4_intergenic_Gm12149|Gm12152_chr11_43653354 |
ACTGCAAATCCCTCGGAGTCTTTCCCCTCGGTTAAACAAAGGGCGGGTCCTTGGGCTGCTGATTAGACTGAGCCAGGTGG GCGGGCGGGGTCAGTCTGGCACTCAGGGTGCACAAACTGGGCAGATG |
| mm4_intron_Ctnnbl1_chr2_157878813 |
ACCTCTTGGGGTTCAGAGGAAGAAATATTATTCATCCGGGGCAGCCTGGCACTCAGGAAAAAACACTTTTGCTGAAAATG AACAGACTAAAGGAAAGGCCTTGCGCCAGGGAAGGATGCTGGACTCAC |
| mm4_intergenic_Gm23888|Cd209g_chr8_4124316 |
CCAGCTGGGCTTGTTGACTATTTACATCCCCCTATGGAGGCATAGAAAGGGTCATAGACCCTCACCTGAGTGCCCAGCTG CTCCCTACCTCCTGCAATATGCACCTCTGCCTTAATTGCAAGTGACTTGAAGGAGAGA |
| mm4_intron_Shroom2_chrX_152759419 |
TGCACTGCAGAAAACGGAGAACTGTGACCACCAGAGTGGCAAGATAACCCCAATATTGCAAAGTGAAGAAAAGCTACTTT GGTGTGTATAAAAATATAAGAAGAGATGTGCCACCACTGAGAAT |
| mm4_intergenic_Mars|Gm6283_chr10_127311822 |
CAACGAGACCCGGAAGTTGACGGTGCGGGCTAGCGTGGCACTCTGGGTCGGCCGGCTCCGAGAGGGTCCTTTGAACTGTT GAAGTCGGCCGCTGTAAAGCGATCCGATGGAGACGACGCGATGGGTGGCATTTTCTG |
| mm4_intergenic_Vapa|Txndc2_chr17_65629049 |
ATGGTTCAGAGCAGGGTTGGCTGCCAGGAGGACATGGGCCTCAGCTTAGCACCCAGGTGGATAAGGCAAGATGACCTCAA GTCTGCGACAAGAGCAGTCTGGGCTCCATAACA |
| mm4_intron_Pphln1_chr15_93483212 |
TATGGTGTCCGTGCCTCTCTCCCTGCCCTTACCTGAGGTCCAGGCTCACCCCCAGCTTCTGACTGGTATACCCAGTACTG ACTTTATCCCTGGAAATGACTAAGCATGGAAAACCCAGTCCCCACCACTCTTCAC |
| mm4_intergenic_Gm15891/Trim42|Gm25607_chr9_97417623 |
TGCAAGTCGGATAATGAGCTAACTCAAACAACTCAAGAAGATGAAGGGACTTACATGACTTGAAGAGTGTCTCCTGAGTG CTAAGAGGACACCCATCTTTCCCAGCCTGCACAGCTATGGGAAGACAGGTGGAACCCAG |
| mm4_intron_Gucy1a3_chr3_82110913 |
TGAAACCAAAGTCTGTGACTGTGAAAGTCTGGGAATCAGCTTCGCACCCAGGTTTATCTAACTCTCAGCAAATTAACAGC AGGAAAGGAGGAATGCTTGAGGCC |
| mm4_intergenic_Fuca1|Hmgcl_chr4_135945254 |
TACAAAAGCAGGCCAGAGCAGGTTAGCAAAAATTCCTGTAAGTGCCTGAGGGTCAAGCTGACCTCAATGTCAGCCCAAGG TCAGGTCAGGATT |
| mm4_intron_Syt9_chr7_107371588 |
ATTTCCACCCGAAGCTGCTCAACCTGAGTGCCCAGTTTCCCCCCAGGCTCTACTGTGGAAAGGACCAGACAAGATCTTGT CTGGGGCCGGGCCCCAGAGTCACACGACTAGAGCGGCTGGGCCTGAACCCTCTCCTTTC |
| mm4_intron_Panx3_chr9_37665969 |
CCAGAAAGACTCAGGAACTAGGGATATGGATGAAACCAAAAACAAGAGTATAAGTTGGTCTGAAACAGACACTGAACAGC CTGTGTGTCAAGCTGACCTGCAGACTTTCTATAATGTACAGCATTTAACCAACCATCCT |
| mm4_intron_Mrp63_chr14_57826428 |
CACTCACTTCCGGCGAGAGGCCGAGCGTGCTCTGGATTCGGCCGCGGGATGTGGGCGCAAGCTTGGCACGCCGGTTGCTC GCTTCCCGGGTCCGGGGCTCAGCGACCGCGGCTTTCGTGTCATTTCC |
| mm3_intergenic_Gpr180|Sox21/Gm4675_chr14_118211528 |
CTGCTGTTTCCCCATGCATGGTATACTGAGCCCTGCTCCTGCGGGCATACTGTGCCCAGCTCCTGTGCGGGTCAGCTTAG CACACAGGCAGCAAAATGCCATGCCTGT |
| mm4_intron_AU040320_chr4_126754159 |
CTGCTTCTAGCCTTGCAGGTGCCAGAGTCCCAATCTGAACCCCTCCGCTTTGGGACTCAGGCCTGGTCAGGCAGCTCCCT CTATTCCCTGACATATGGGCAGACC |
| mm4_intron_Csrp1_chr1_135735623 |
CTGGTCTGAGGCTTCCTTCGTGTTGGGCTGAGCTGGGCACTCAGGATTGAACAAAGACTGAATTGACAGCTTCCGGTCTG TGGGGTCAGACAAACCAGCTGAGGTCAAGCAAGCTGAGG |
| mm4_intron_Trio_chr15_27854722 |
TGCCCCTGGTACTCCATACACTCTGTCCTTGCTGCCTGCACTGTCCCCCTGGTCTTCTCCCTGGCCCCCTGCAGTCTCCC ACCCAGAGTGCAGAGCTGACCACCGGGCCTCCCCAATGGCCAAGGAAGTGT |
| mm4_intergenic_Gm10660|Nat1_chr8_67437859 |
ACCATGAACTTCCCGACACCTATACCTGAGAGCCAAGATAAACCCCATTTTTTCATTAGCAATTTCACAGTCTCACCATA GCAACA |
| mm4_intergenic_n-R5s30|Rbfox1_chr16_5625300 |
TTGGAAAAGCAGAGCCCACAGCGAGGCCCTGGGGTCTGGGGGTCTCCCTGGCACCCAGGGACTGGGGGTGTGTGATTTAT TAGAATGTGCAGAGCTCTCTTGGCTT |
| mm4_intron_Os9_chr10_127097726 |
GCACAGAGCCCCTTCTTCATGATGAGCTTTTCAGCTTCTACACTCATGCTCATTTAGTCTTTGCCTGTGTGCCAGGCAGA CCACCAGGCAGTGGGCATAGAGCAAACCAGG |
| mm4_intron_Lepre1_chr4_119238862 |
AGACTGGCTTCTGGCTGAACCCAATCAGAAGGAAGATGAGTGCTCANNNNNNNNNNNNNNNNNNNNCCGGTCTTGGACTC AGCTTGTCACTCAGGGTGGGAAGGAGTACCAGGAGT |
| mm4_exon_Ppm1l_chr3_69317651 | CTGAGACCCGAGACGCTTTTCCTGCTGTGCATCAGCTTGGCTCTCTGGAGTTACTTCTTCCACACGGACGAAGTGAAG |
| mm4_intergenic_Sox5|Sox5/4933415D12Rik_chr6_143963142 |
ACCTGACACCGTATTACTCACTAATCTTTCCATACAATAACTAATTCAGCAACCTAGAAAATATTTAATAACTACCAACT ATCTATGTGTGTGGAGTTGAGCTTGGCAGTCAGGATATGATCACCAAGAAAACCAAGATT |
| mm4_intron_Gm2956_chr14_3333062 |
GGGGTTCACCTCTTGACCATGCGCTGGCAGTCACCTGGGCACTCAGGTTCCTGAGGACCGCCGCCTGGGAAGGCCCGCGG CTGCCAGAAGCCCAGAGCCACGCCTCCGACTCGGGTAGCCTAGGCTGCCCTGAACTGTTC |
| mm4_intron_Gm2237_chr14_19584683 |
GAACAGTTCAGGGCAGCCTAGGCCACCCGAGTCGGAGGCGTGGCTCTGGGTTTCTGGCAGCCTCGGGCCTTCCCAGGCGG AGGTTCTTGGGAACCTGAGTGCCCAGGTGACTGCCAGCGCATGGTCAAGAGGTGAACCCC |
| mm4_intergenic_Gm26680|Gm8362_chr14_6756572 |
GAACAGTTCAGGGCAGCCTAGGCCACCCGAGTCGGAGGCGTGGCTCTGGGCTTCTGGCAGCCTCGGGCCTTCCCAGGCGG CGGTTCTTGGGAACCTGAGTGCCCAGGTGACTGCCAGCGCATGGTCAAGAGGTGAACCCC |
| mm4_intron_Grik5_chr7_25050091 |
GCACAGAGCTGAGATCCACACACGGCCTGCACACCCCAGAGCTCTGCTGGGTCCTGTGTGCCAAACAGACCTCCAAATCT GAGACGGAGGGGCCTGGGCTCCATCTGTCCACAGCAGTCTGAGCTT |
| mm4_intron_Gpr125_chr5_50049123 |
AAGTGCACAGATAAGGCGCTGGGAGTGACTGGCATGGGCCCGAGAGCCGAGCTGACTCACAGTCTGGCAAAGGAGCTTTG TGCTCGCAATCTCC |
| mm4_intergenic_Onecut1|Onecut1/Gm20649_chr9_74870611 |
GGCAAGGCCCATCTCATTAGATATGCTCTGCGGGTAAGCCTGGCCCTCTGGGACCCGTGCCTGCAGAAAGGCCCAAAGGC CCACGGCCACCCGTGCTTCCAAGGCTGACGTTCT |
| mm4_intergenic_Pou3f4|Cylc1_chrX_110851350 |
AAGTGAAAACCCACTCGGCTTTTGAGTCAGCTTGGCTCTCTGGAGAGCTGCACAAACAGCTGAAACTGTTTACATGAGAG GCTAACAGCGG |
| mm4_intergenic_Arhgap26|Fgf1_chr18_38804352 | GAATCGCTTCCTGCAGCATGTGAGCATGAAATCCGTAGTCCCAAGCTGAACCCAAATGTGGAGGGACAAACACCTG |
| mm4_exon_Aim1l_chr4_134081174 |
GCAGAGCTGGGAAGTAAGCAGAGACATCTACAACCTTCAGCAGCCTGAGGACAGCCAGAGTCCCCAGCTGACCTCCGTGG GGTCCCTGCGAATCCTTGGGGGCTGGTGAGGATTGAAG |
| mm3_exon_Slc9a1_chr4_133417933 |
TCCTGACCTGGTTCATCAACAAGTTCCGCATTGTCAAGCTGACCCCCAAGGACCAGTTCATCATTGCCTATGGGGGCCTG CGAGGGGCCATCGCCTTCTCGCTGGGCTACCTCCTGGACAAGAAGCACTTCCCC |
| mm4_intron_Usp48_chr4_137601301 |
TGGGCATGACTTCCTTGTGTTTTGTGGAGGATGGAAAAAGATGGGGAAGCCTGCCTGCATCAGCAGCAGGACGCCTAAGT GCCAAACTGTCCACCAGAGCTGCTTTCTGAAAGACCCGTAGGACTGCC |
| mm4_intergenic_Plekhb1|Fam168a_chr7_100686662 |
CTCCACCTATCAGAACAGTGCATGAGGGGCTCAGCTTGTCACTTGGGGCCTTCCTAGCTCTCCCTTGAGCCACATTCATC CTGAGGTTGCCTGAGG |
| mm4_intron_Sytl3_chr17_6734806 |
AGCAGAAGGACAAGGCCTTCAGTGCACACCTCATCCTCTCTAAGGTGTAGACTGCCAGTCTCTGGTGGCCCTGTAGATAA AAGCAGGCATCCAGGGGTCAGCTTGGCTCTTTGGGAACCAAGAGGGCTTCTGGG |
| mm4_intergenic_Nkd2|Trip13_chr13_73888644 |
GAGTGCAGTCAGGACCCAAAGTACTCAGTTCTCTGATTTGGGGGTCAGATCAGCACTGAGGGCAGCTGCAGATCCAAACA GATGTAGTTGTTCTGGAATCAGGAGCTGCCATTGAAGG |
| mm4_intron_Cacna2d3_chr14_29638505 |
CTTACACTTCCTCCCTGCCCCCAGAGAGCCAAGCTGACACCATTCTGAACACATCTCTCCAATAACTTTCACTTTTACTT CTATCTTGATGTTAGTATGCCTTCAAATCTAGTTTTCCCCTTTATCAGCAGGAAATGT |
| mm4_intergenic_Ube2cbp|Dopey1_chr9_86465039 | GCTCTAGTAGGACAGGTGCGACCCAGAGAGCAGAGCTGACCTCCACTGGAAGGATGTTGGATGGGC |
| mm4_intron_Ap3s2_chr7_79902466 |
AAGATGTGTGTAGGCCTGCCTCATCCAAGAAACTCACCTTGGGCTGGGGTGGGGCTCAGCTCAGCACTGTGGTTACCCTG TATGTGCGGGGCCTTGGGTTCCTGTCCTGCACTA |
| mm4_exon_C77080_chr4_129222083 |
AGTTCAGCCACCAGATTCCGCTGGAGGTCAGCCTGGGCCTCGGGGGACACGGGCCTCTCCAGCTGCAGTTTTTTGGTGCC CTTGGAGAAGATGAAGCTGGCCTTGGAGGCAGGAGACTGTG |
| mm4_intergenic_Gm26861|Gm25496_chr13_11219462 |
GCTCTGCTGCCTCATGGAATTGTAGTTAATATATGGTGATTTCTGGGGGTAAGCTTGACTCCCTGGCACACTGGAGTGGA GCTAATGAAGTAAAACGATTGTGGCGCACATTGAGTGGATTAGGCTGGGTAAGGCTGC |
| mm4_intergenic_1700009N14Rik|Gm12385_chr4_39577194 |
TGATACTCAGATATGGGCATTTGCCCAGCATGGGCGTAAGCTAGGCACTGGGGTTAATGTCTAGCAACACAGATCATAGC ACAGA |
| mm4_intron_Gm10619_chr7_73815807 |
CCCAAAGGTTTGAGACGACACTGTTGTCATTCTGGCAGAACGTCCTGAATGACAACCTGTCCCCCATGAATCCCAGTCAA TGGTTTGGCATCCT |
| mm4_intergenic_Gm25115|Diap3_chr14_86440731 |
TGGTACTGTTTCAGCGGAGACTTGAAGACAAACTTCAGCAATGCCAAGCATGTAGAATGGAAAGACCAGATTGGGGGTCA GGCTTGCACACAGGGAAAATTACCCTCTTCCCTTTGAGGAAACTCAGCA |
| mm4_intergenic_Asic2|4930527B05Rik_chr11_81322083 |
TCAGTGAGCATGATCTCTGTGTATTTGGTGGTCAACTTTGGACTCTGGTGAGAGCTTTGAACTGTATTGAGCATTACTGT CATGTGTGAACCCCTTCCCATTCCTTTTATCAGGTCTCTGCTCTGATGGCTGCTTGGACA |
| mm4_intron_Grb10_chr11_11999875 |
AACCTACTTATTTGTCTAGTGAGGTCCAAAATGGCAACCTGACCCCCAGAATGTGTTTAGTACATGGATGATACTCAGTA TCAACTTATAATCTTTGATTAGCTTCTAAGATGTGGTCATCTTGATGCTGAGT |
| mm4_intron_Slc26a9_chr1_131765051 |
TCCTCTAGCTAAGCTCCACCTATGAGAAGATCGGAGTCCAGATCTTTCTGGTAAACATCCACGGTAAGAAAACTGGGGGT CAGGTGGGCATTTAGGGGACCAGAGGCTGGAAAGGGATGCAGTGGGGAC |
| mm4_intron_Rab8a_chr8_72169574 |
GTGTGTGGACCTGAAACCCACAAACAACCTTAAAGCTAAGGCCAGACCCAGCAGCCAGAAGTACTCATAGTCCTCAGCCC TGGGGGTAACCTTGGCAGCCAGGGTCAATTAGGAAACTGTGAGCATCTAGGACCCCA |
| mm4_intron_1700060C16Rik_chr6_143635139 |
TCTCTCCCCATTCCTCCAGGTGTGACAAGAAAGCCCCACCAGATGTGGGTGGCAGCTTGGCAGCCTGGCCAGGCAGGGAC CAAAGAGCTGAGTGTGCAAGATGAAGGCTAAGTGTCGAGGGCTA |
| mm4_intergenic_Haus8/Myo9b|Myo9b_chr8_71274592 |
ATGCTCTGGCTTTGACCTCCTACTTGGGTAAAACCCTCTCTCAAGTCTAGCTAGTACCTTGTGGGTCAGGTGGGGACTCA GGACTCCTGGGCTTGACTCCCTTCCTGAC |
| mm4_intron_Muc5ac_chr7_141795801 |
ACCAAGGTTCAGTGAGGGAGATATTGCCTCAGTCCCAAACTGACCCCCTTGGCTCTGGAATTGGTTATAGGAGATCATGG TGAAAGCTACTGGAGAGGTCTTTGTGAACCAGATCTACACCCAGTTGCCAGTG |
| mm4_intron_Cog7_chr7_121936445 |
GCAAATGCAGCAGCTAGTGGTGGGCCAAGGTGCAGAGTCGGGGAGACTTTGAGGAAACATCATGAGGGCCTCAGTGCACA GCTCACCCCCAGAGCACTGCACCCATCAGAAGAGC |
| mm4_intergenic_Pkdrej|Ttc38_chr15_85831516 |
TGAATTTTCCAGCCTCTCCACCCTCACTGCCCAGCTGACTCCCACAAAATGTCTGGAAGCTCCTACAGAGAGGAAGGGGA GAGGGAGACTGT |