CRISPOR

DNA break repair outcome predictions

Guide: TTAGAAAGAAGAGGGCAATG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TGCAGCAGGAGGATGACAGCTTAGAAAGAAGAGGGCAATGGGGCTTCCTCCCAGAGGCGGTGCGGCACAGAGGAGCGCTC`	(Wild-type sequence)
536	`GGATGACAGCTTAGAAAGAAGAGGGC--------TTCCTCCCAGAGGCGGTGCGGCACAG`	`8 bp deleted → frameshift`
282	`GGATGACAGCTTAGAAAGAAGAGGG-------GCTTCCTCCCAGAGGCGGTGCGGCACAG`	`7 bp deleted → frameshift`
255	`GGATGACAGCTTAGAAAGAAGAGG---------CTTCCTCCCAGAGGCGGTGCGGCACAG`	`9 bp deleted → no frameshift`
253	`GGATGACAGCTTAGAAAGAAGAGG-----------------------CGGTGCGGCACAG`	`23 bp deleted → frameshift`
199	`GGATGACAGCTTAGAAAGAAGAGGGC----------------------GGTGCGGCACAG`	`22 bp deleted → frameshift`
180	`GGATGACAGCTT------------------------CCTCCCAGAGGCGGTGCGGCACAG`	`24 bp deleted → no frameshift`
156	`GGATGACAGCTTAGAAAGAAGAGGGCA------------------------------CAG`	`30 bp deleted → no frameshift`
134	`GGATGACAGCTTAGAAAGAAGAGGGCA----------------GAGGCGGTGCGGCACAG`	`16 bp deleted → frameshift`
114	`GGATGACAGCTTAGAAAGAAGAGGG-------------------------TGCGGCACAG`	`25 bp deleted → frameshift`
109	`GGATGACAGCTTAGAAAGAAGAGG--------------------------TGCGGCACAG`	`26 bp deleted → frameshift`
109	`GGATGACAGCTTAGAAAGA--------------------------GGCGGTGCGGCACAG`	`26 bp deleted → frameshift`
105	`GGATGACAGCTTAGAAAGAAGAG---------------------AGGCGGTGCGGCACAG`	`21 bp deleted → no frameshift`
103	`GGATGACAGCTTAGAAAGAAGAGGGC---------------------------GGCACAG`	`27 bp deleted → no frameshift`
99	`GGATGACAGCTTAGAAAGAAGAGGGCAATG----------------------CGGCACAG`	`22 bp deleted → frameshift`
89	`GGATGACAGCTTAGA------------------------------GGCGGTGCGGCACAG`	`30 bp deleted → no frameshift`
89	`GG------------------------------GCTTCCTCCCAGAGGCGGTGCGGCACAG`	`30 bp deleted → no frameshift`
87	`GGATGACAG-----------------------------------AGGCGGTGCGGCACAG`	`35 bp deleted → frameshift`
86	`GGATGACAGCTTAGAAAGAAG-------------------------GCGGTGCGGCACAG`	`25 bp deleted → frameshift`
84	`GGATGACAGCTTAGAAAGAAGAGG-------------------------------CACAG`	`31 bp deleted → frameshift`
84	`GG-------------------------------CTTCCTCCCAGAGGCGGTGCGGCACAG`	`31 bp deleted → frameshift`
74	`GGATGACAGCTTAGAAAG----------------------------GCGGTGCGGCACAG`	`28 bp deleted → frameshift`
74	`GGATGACAGCT----------------------------CCCAGAGGCGGTGCGGCACAG`	`28 bp deleted → frameshift`
60	`GGATGACAGCTTAGAAAGAAGAGGGCA--------------------------------G`	`32 bp deleted → frameshift`
60	`GGATGACAGCTTAG--------------------------------GCGGTGCGGCACAG`	`32 bp deleted → frameshift`
60	`GGATGACAGC--------------------------------------GGTGCGGCACAG`	`38 bp deleted → frameshift`
47	`GGATGACAGCTTAGAAAGAAGAG-------------------------------------`	`37 bp deleted → frameshift`
47	`GGATGACAG-------------------------------------GCGGTGCGGCACAG`	`37 bp deleted → frameshift`
46	`GGATGACAG---------------------------------------------------`	`51 bp deleted → no frameshift`
46	`GGATGACAGC-------------------------------------------GGCACAG`	`43 bp deleted → frameshift`
42	`GGATGACAGCTTAGAAAGAAG---------------------------------------`	`39 bp deleted → no frameshift`
42	`GGATGA---------------------------------------GGCGGTGCGGCACAG`	`39 bp deleted → no frameshift`
42	`GG---------------------------------------------CGGTGCGGCACAG`	`45 bp deleted → no frameshift`
40	`GGATGACAGC----------------------------------------------ACAG`	`46 bp deleted → frameshift`
36	`GGATGACAGCTTAGAAAG------------------------------------------`	`42 bp deleted → no frameshift`
36	`GGA------------------------------------------GGCGGTGCGGCACAG`	`42 bp deleted → no frameshift`
36	`GG------------------------------------------------TGCGGCACAG`	`48 bp deleted → no frameshift`
30	`GGATGACAGCTTAG----------------------------------------------`	`46 bp deleted → frameshift`
28	`GGATG-----------------------------------------------CGGCACAG`	`47 bp deleted → frameshift`
28	`GG-----------------------------------------------------CACAG`	`53 bp deleted → frameshift`
25	`GGATGACA-------------------------------------------------CAG`	`49 bp deleted → frameshift`

Version 5.01 - Documentation - Contact us - Downloads/local installation - Citation - License