CRISPOR

DNA break repair outcome predictions

Guide: GCCAGAGAGGCTGTCCGCGG AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GAGCTACTAGGTTGCCCTTGGCCAGAGAGGCTGTCCGCGGAGGGGACTGGGGGTCCCGGGCCAAGCATGGCCGCTCCAGC`	(Wild-type sequence)
344	`GTTGCCCTTGGCCAGAGAGGCTGTCCGCGG---GGACTGGGGGTCCCGGGCCAAGCATGG`	`3 bp deleted → no frameshift`
327	`GTTGCCCTTGGCCAGAGAGGCTGTCCGCGG----GACTGGGGGTCCCGGGCCAAGCATGG`	`4 bp deleted → frameshift`
311	`GTTGCCCTTGGCCAGAGAGGCTGTCCGCGG-----ACTGGGGGTCCCGGGCCAAGCATGG`	`5 bp deleted → frameshift`
261	`GTTGCCCTTGGCCAGAGAGG-------------GGACTGGGGGTCCCGGGCCAAGCATGG`	`13 bp deleted → frameshift`
257	`GTTGCCCTTGGCCAGAGAGGCTGTCC--------------------CGGGCCAAGCATGG`	`20 bp deleted → frameshift`
242	`GTTGCCCTTGGCCAGAGAGGCTGTCCGCGG----------GGGTCCCGGGCCAAGCATGG`	`10 bp deleted → frameshift`
232	`GTTGCCCTTGGCCAGAGAGGCTGTCCGCGG-------------------GCCAAGCATGG`	`19 bp deleted → frameshift`
230	`GTTGCCCTTGGCCAGAGAGGCTGTCCGCGG-----------GGTCCCGGGCCAAGCATGG`	`11 bp deleted → frameshift`
224	`GTTGCCCTTGGCCAGAGAGGCTG----------------GGGGTCCCGGGCCAAGCATGG`	`16 bp deleted → frameshift`
219	`GTTGCCCTTGGCCAGAGAGGCTGTCCGCGG------------GTCCCGGGCCAAGCATGG`	`12 bp deleted → no frameshift`
210	`GTTGCCCTTGGCCAGAGAGGCTGTCCG---------------------GGCCAAGCATGG`	`21 bp deleted → no frameshift`
208	`GTTGCCCTTGGCCAGAGAGGCTGTCCGCGG-------------TCCCGGGCCAAGCATGG`	`13 bp deleted → frameshift`
198	`GTTGCCCTTGGCCAGAGAGG--------------GACTGGGGGTCCCGGGCCAAGCATGG`	`14 bp deleted → frameshift`
188	`GTTGCCCTTGGCCAGAGAGG---------------ACTGGGGGTCCCGGGCCAAGCATGG`	`15 bp deleted → no frameshift`
147	`GTTGCCCTTGGCCAGAGAGGCTGTCCGCGG--------------------CCAAGCATGG`	`20 bp deleted → frameshift`
147	`GTTGCCCTTGGCCAGAGAGG--------------------GGGTCCCGGGCCAAGCATGG`	`20 bp deleted → frameshift`
141	`GTTGCCCTTGGCCAGAG---------------GGGACTGGGGGTCCCGGGCCAAGCATGG`	`15 bp deleted → no frameshift`
140	`GTTGCCCTTGGCCAGAGAGG---------------------GGTCCCGGGCCAAGCATGG`	`21 bp deleted → no frameshift`
133	`GTTGCCCTTGGCCAGAGAGGC------------------------------CAAGCATGG`	`30 bp deleted → no frameshift`
133	`GTTGCCCTTGGCCAGAGAGG----------------------GTCCCGGGCCAAGCATGG`	`22 bp deleted → frameshift`
133	`GTTGCCCTTGG----------------------GGACTGGGGGTCCCGGGCCAAGCATGG`	`22 bp deleted → frameshift`
128	`GTTGCCCTTGGCCAG-----------------GGGACTGGGGGTCCCGGGCCAAGCATGG`	`17 bp deleted → frameshift`
127	`GTTGCCCTTGGCCA---------------------------------------AGCATGG`	`39 bp deleted → no frameshift`
126	`GTTGCCCTTGGCCAGAGAGGCTGTCCGC-----------------------CAAGCATGG`	`23 bp deleted → frameshift`
126	`GTTGCCCTTGGCCAGAGAGG-----------------------TCCCGGGCCAAGCATGG`	`23 bp deleted → frameshift`
126	`GTTGCCCTTGG-----------------------GACTGGGGGTCCCGGGCCAAGCATGG`	`23 bp deleted → frameshift`
122	`GTTGCCCTTGGCCAGAGA------------------CTGGGGGTCCCGGGCCAAGCATGG`	`18 bp deleted → no frameshift`
120	`GTTGCCCTTGG------------------------ACTGGGGGTCCCGGGCCAAGCATGG`	`24 bp deleted → no frameshift`
117	`GTTGCCCTTGG-----------------------------GGGTCCCGGGCCAAGCATGG`	`29 bp deleted → frameshift`
110	`GTTGCCCTTGGCCAGA--------------------CTGGGGGTCCCGGGCCAAGCATGG`	`20 bp deleted → frameshift`
109	`GTTGCCCTTGGCCAGAGAGGCTGTCC--------------------------AAGCATGG`	`26 bp deleted → frameshift`
98	`GTTGCCCTTGGCCAGAGAGGCTGTCCGC----------------------------ATGG`	`28 bp deleted → frameshift`
94	`GTTGCCCTTGGCCAGAGAGG-----------------------------GCCAAGCATGG`	`29 bp deleted → frameshift`
89	`GTTGCCCTTGGCCAGAGAGGCTGTCCGCGG------------------------------`	`30 bp deleted → no frameshift`
89	`GTTGCCCTTGG------------------------------GGTCCCGGGCCAAGCATGG`	`30 bp deleted → no frameshift`
84	`GTTGCCCTTGG-------------------------------GTCCCGGGCCAAGCATGG`	`31 bp deleted → frameshift`
81	`GTTGCCC----------------------------------------GGGCCAAGCATGG`	`40 bp deleted → frameshift`
80	`GTTGCCCTTGG--------------------------------TCCCGGGCCAAGCATGG`	`32 bp deleted → frameshift`
76	`GTTGCCCTTGGCC---------------------------------CGGGCCAAGCATGG`	`33 bp deleted → no frameshift`
73	`GTTGCCCTTGGCC----------------------------------GGGCCAAGCATGG`	`34 bp deleted → frameshift`
69	`GTTGCCCTTGGCCAGAGAGGC-----------------------------------ATGG`	`35 bp deleted → frameshift`
66	`GTTGCCCT------------------------------GGGGGTCCCGGGCCAAGCATGG`	`30 bp deleted → no frameshift`
60	`GTTGCCCTTGG--------------------------------------GCCAAGCATGG`	`38 bp deleted → frameshift`
60	`GTTGCC----------------------------------------------AAGCATGG`	`46 bp deleted → frameshift`
56	`GTTGCCC---------------------------------------CGGGCCAAGCATGG`	`39 bp deleted → no frameshift`
54	`GTTGCCCTTGGCCAGAGAGG----------------------------------------`	`40 bp deleted → frameshift`
52	`GTTG-----------------------------------GGGGTCCCGGGCCAAGCATGG`	`35 bp deleted → frameshift`
51	`GTTGCC-----------------------------------------GGGCCAAGCATGG`	`41 bp deleted → frameshift`
49	`GTTGCCCTTGGCCAGAGAGGCTG------------------------------------G`	`36 bp deleted → no frameshift`
49	`GTTGCCCTTGGCCAGAGAG------------------------------------CATGG`	`36 bp deleted → no frameshift`
45	`GTTGCCCTTGGCCAGAG--------------------------------------CATGG`	`38 bp deleted → frameshift`
44	`GTTGCCCTTGGC--------------------------------------------ATGG`	`44 bp deleted → frameshift`
43	`GTTGCCCTTGG-------------------------------------------------`	`49 bp deleted → frameshift`
42	`GTTGCCC---------------------------------------------AAGCATGG`	`45 bp deleted → no frameshift`
40	`GTTGCCCTTGGCCAG----------------------------------------CATGG`	`40 bp deleted → frameshift`
36	`GT------------------------------------------CCCGGGCCAAGCATGG`	`42 bp deleted → no frameshift`
34	`GTTGCCCTTGGCCA-------------------------------------------TGG`	`43 bp deleted → frameshift`
31	`GTTGC---------------------------------------------------ATGG`	`51 bp deleted → no frameshift`
19	`GTTG-------------------------------------------------------G`	`55 bp deleted → frameshift`

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