CRISPOR

DNA break repair outcome predictions

Guide: GGTTGCCTGAGCCCGCGAGC CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CAGAGAAGATGGTGCCCCACGGTTGCCTGAGCCCGCGAGCCGGCCCTCCGACTTCCCGGGAGCGTGGCGGGGGAGGCCAG`	(Wild-type sequence)
439	`GGTGCCCCACGGTTGCCTGAGCCC------------TCCGACTTCCCGGGAGCGTGGCGG`	`12 bp deleted → no frameshift`
327	`GGTGCCCCACGGTTGCCTGAGCCCGCGAGC----CCTCCGACTTCCCGGGAGCGTGGCGG`	`4 bp deleted → frameshift`
311	`GGTGCCCCACGGTTGCCTGAGCCCGCG-----GCCCTCCGACTTCCCGGGAGCGTGGCGG`	`5 bp deleted → frameshift`
283	`GGTGCCCCACGGTTGCCTGAGCCCG---------------ACTTCCCGGGAGCGTGGCGG`	`15 bp deleted → no frameshift`
282	`GGTGCCCCACGGTTGCCTGAGCCCG-------GCCCTCCGACTTCCCGGGAGCGTGGCGG`	`7 bp deleted → frameshift`
268	`GGTGCCCCACGGTTGCCTGAGCCCGC--------CCTCCGACTTCCCGGGAGCGTGGCGG`	`8 bp deleted → frameshift`
261	`GGTGCCCCACGGTTGCCTGAGCCCGCGA-------------CTTCCCGGGAGCGTGGCGG`	`13 bp deleted → frameshift`
253	`GGTGCCCCACGGTTGCCTGAGCCCG-----------------------GGAGCGTGGCGG`	`23 bp deleted → frameshift`
244	`GGTGCCCCACGGTTGCC------------------CTCCGACTTCCCGGGAGCGTGGCGG`	`18 bp deleted → no frameshift`
230	`GGTGCCCCACGGTTGCCTGAGCCC-----------CTCCGACTTCCCGGGAGCGTGGCGG`	`11 bp deleted → frameshift`
221	`GGTGCCCCACGGTTGCCTGAGCCCGCGAGC-----------------------GTGGCGG`	`23 bp deleted → frameshift`
210	`GGTGCCCCACGG---------------------CCCTCCGACTTCCCGGGAGCGTGGCGG`	`21 bp deleted → no frameshift`
208	`GGTGCCCCACGGTTGCCTGAGCC-------------TCCGACTTCCCGGGAGCGTGGCGG`	`13 bp deleted → frameshift`
193	`GGTGCCCCACGGTTGCCT-------------------CCGACTTCCCGGGAGCGTGGCGG`	`19 bp deleted → frameshift`
188	`GGTGCCC-----------------------------TCCGACTTCCCGGGAGCGTGGCGG`	`29 bp deleted → frameshift`
179	`GGTGCCCCACGGTTGCCTGAGCC----------------GACTTCCCGGGAGCGTGGCGG`	`16 bp deleted → frameshift`
155	`GGTGCCCCACGGTTGCCTGAGCCCGCG---------------------------TGGCGG`	`27 bp deleted → no frameshift`
148	`GGTGCCCCACGGTTGCCTGAGC-------------------------------GTGGCGG`	`31 bp deleted → frameshift`
148	`GGTGCCCC----------------------------TCCGACTTCCCGGGAGCGTGGCGG`	`28 bp deleted → frameshift`
140	`GGTGCCCCACGGTTGCCTGAGCCCGCG---------------------GGAGCGTGGCGG`	`21 bp deleted → no frameshift`
133	`GGTGCCCCACGGTTGCCTGAGCCC----------------------CGGGAGCGTGGCGG`	`22 bp deleted → frameshift`
133	`GGTGCCCCACGGTTGCC----------------------GACTTCCCGGGAGCGTGGCGG`	`22 bp deleted → frameshift`
121	`GGTGCCCCACGGTTGCCTGAGCCCGCG--------------------------------G`	`32 bp deleted → frameshift`
120	`GGTGCCCCACGGTTGCCTGAGCC------------------------GGGAGCGTGGCGG`	`24 bp deleted → no frameshift`
105	`GGTGCCCCACGGTTGCCTGA---------------------CTTCCCGGGAGCGTGGCGG`	`21 bp deleted → no frameshift`
103	`GGTGCCCC---------------------------CTCCGACTTCCCGGGAGCGTGGCGG`	`27 bp deleted → no frameshift`
98	`GGTGCCCCACGGTTGCCTGAGCCCGCGAGC----------------------------GG`	`28 bp deleted → frameshift`
94	`GGTGCCCCACGG-------------------------------------GAGCGTGGCGG`	`37 bp deleted → frameshift`
94	`GGTGCCCCACGGTTGCCTGAGCCCG-----------------------------TGGCGG`	`29 bp deleted → frameshift`
94	`GGTGCCCCACGGTTGCC-----------------------------CGGGAGCGTGGCGG`	`29 bp deleted → frameshift`
94	`GGTGCCCCACG-----------------------------ACTTCCCGGGAGCGTGGCGG`	`29 bp deleted → frameshift`
89	`GGTGCCCCACGGTTGCC------------------------------GGGAGCGTGGCGG`	`30 bp deleted → no frameshift`
89	`GGTGCC------------------------------TCCGACTTCCCGGGAGCGTGGCGG`	`30 bp deleted → no frameshift`
86	`GGTGCCCCACGGTTGCCT-------------------------TCCCGGGAGCGTGGCGG`	`25 bp deleted → frameshift`
85	`GGTGCCCC---------------------------------------GGGAGCGTGGCGG`	`39 bp deleted → no frameshift`
84	`GGTGCCCC-------------------------------GACTTCCCGGGAGCGTGGCGG`	`31 bp deleted → frameshift`
84	`GG-------------------------------CCCTCCGACTTCCCGGGAGCGTGGCGG`	`31 bp deleted → frameshift`
81	`GGTGCCC----------------------------------------GGGAGCGTGGCGG`	`40 bp deleted → frameshift`
80	`GGTGCCC--------------------------------GACTTCCCGGGAGCGTGGCGG`	`32 bp deleted → frameshift`
76	`GGTGCC---------------------------------GACTTCCCGGGAGCGTGGCGG`	`33 bp deleted → no frameshift`
73	`GGTGCCCCACGGTTGCCTGAGCCCG----------------------------------G`	`34 bp deleted → frameshift`
66	`GGTGCCCCACGGTTGCCTGAGC------------------------------------GG`	`36 bp deleted → no frameshift`
62	`GGTGCCCCACGGTTGC-------------------------------------GTGGCGG`	`37 bp deleted → frameshift`
60	`GGTGCCCCAC--------------------------------TTCCCGGGAGCGTGGCGG`	`32 bp deleted → frameshift`
60	`GGTGCCCCACGG--------------------------------------AGCGTGGCGG`	`38 bp deleted → frameshift`
60	`GGTGCCCC--------------------------------------CGGGAGCGTGGCGG`	`38 bp deleted → frameshift`
54	`GGTGCCCCACGG------------------------------------------------`	`48 bp deleted → no frameshift`
51	`GGTGCC-----------------------------------------GGGAGCGTGGCGG`	`41 bp deleted → frameshift`
48	`GGTGCCCCACGGTTGC------------------------------------------GG`	`42 bp deleted → no frameshift`
47	`GGTGCCCCACGGTTGCCTG-------------------------------------GCGG`	`37 bp deleted → frameshift`
46	`GGTGCCCCACG-------------------------------------------TGGCGG`	`43 bp deleted → frameshift`
44	`GGTGCCCCACGGTT------------------------------CCCGGGAGCGTGGCGG`	`30 bp deleted → no frameshift`
42	`GGTGCCCCACGG---------------------------------------------CGG`	`45 bp deleted → no frameshift`
38	`GGTGCCCCACGGTTG-----------------------------------------GCGG`	`41 bp deleted → frameshift`
38	`GG-----------------------------------------------GAGCGTGGCGG`	`47 bp deleted → frameshift`
37	`GGTG----------------------------------------------------GCGG`	`52 bp deleted → frameshift`
36	`GGTGCCCCACGGT------------------------------------------GGCGG`	`42 bp deleted → no frameshift`
36	`GGTGC------------------------------------------------GTGGCGG`	`48 bp deleted → no frameshift`
36	`GG------------------------------------------------AGCGTGGCGG`	`48 bp deleted → no frameshift`
28	`GGTGC-----------------------------------------------------GG`	`53 bp deleted → frameshift`
25	`GG-------------------------------------------------------CGG`	`55 bp deleted → frameshift`
22	`GG----------------------------------------------------------`	`58 bp deleted → frameshift`

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