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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313824_F | TCGTCGGCAGCGTCAGGGAGCCTGGGATTAGGAG | 60.1 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313824_R | GTCTCGTGGGCTCGGACCATGTCCTTTCGGCTCTC | 59.7 | 1.00 |
| mm4_intron_Capn8_chr1_182627895_F | TCGTCGGCAGCGTCGAAGACAAAGCACGCCACTG | 60.0 | 0.66 |
| mm4_intron_Capn8_chr1_182627895_R | GTCTCGTGGGCTCGGCATTGACAGTTCCGTCCCCA | 59.9 | 0.66 |
| mm4_intergenic_9430023L20Rik|6030408B16Rik_chr15_101292140_F | Primer3: not found at this Tm | N.d. | 0.66 |
| mm4_intergenic_9430023L20Rik|6030408B16Rik_chr15_101292140_R | Primer3: not found at this Tm | N.d. | 0.66 |
| mm4_intergenic_Gyg|Cpa3_chr3_20191587_F | TCGTCGGCAGCGTCTCCAATTCTATTCCCTTCTCAGCA | 59.5 | 0.64 |
| mm4_intergenic_Gyg|Cpa3_chr3_20191587_R | GTCTCGTGGGCTCGGGATTCCTGGGCTGCAGATCA | 59.8 | 0.64 |
| mm4_intron_Rgs6_chr12_83020535_F | Primer3: not found at this Tm | N.d. | 0.50 |
| mm4_intron_Rgs6_chr12_83020535_R | Primer3: not found at this Tm | N.d. | 0.50 |
| mm2_exon_Nynrin/Gm26751_chr14_55871433_F | Primer3: not found at this Tm | N.d. | 0.50 |
| mm2_exon_Nynrin/Gm26751_chr14_55871433_R | Primer3: not found at this Tm | N.d. | 0.50 |
| mm4_intergenic_Gm21392|Gm23727_chr6_78549423_F | Primer3: not found at this Tm | N.d. | 0.46 |
| mm4_intergenic_Gm21392|Gm23727_chr6_78549423_R | Primer3: not found at this Tm | N.d. | 0.46 |
| mm4_intron_Pik3c2g_chr6_139912550_F | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intron_Pik3c2g_chr6_139912550_R | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intron_Xrcc5_chr1_72310267_F | TCGTCGGCAGCGTCACCCCTCTGTCTATGTGGCT | 59.9 | 0.41 |
| mm4_intron_Xrcc5_chr1_72310267_R | GTCTCGTGGGCTCGGTCCCTGTCCTGCTACGTAAA | 58.0 | 0.41 |
| mm4_intergenic_Plk2|Actbl2_chr13_110422226_F | TCGTCGGCAGCGTCGGTCCCCTCTGTCCCTGTAT | 60.0 | 0.38 |
| mm4_intergenic_Plk2|Actbl2_chr13_110422226_R | GTCTCGTGGGCTCGGTCAGCCCCAGGACCTAACTA | 59.2 | 0.38 |
| mm4_intergenic_Atp6v1b2|Lzts1_chr8_69115924_F | TCGTCGGCAGCGTCAGCAACACAGGTCACACACA | 60.0 | 0.37 |
| mm4_intergenic_Atp6v1b2|Lzts1_chr8_69115924_R | GTCTCGTGGGCTCGGAGCTGATGCAGAGCTTGACA | 59.6 | 0.37 |
| mm4_intron_Arhgef3_chr14_27399347_F | TCGTCGGCAGCGTCGCTCCAACCCAGCTTCTCAT | 60.0 | 0.37 |
| mm4_intron_Arhgef3_chr14_27399347_R | GTCTCGTGGGCTCGGTGCTGGGCTCTGACTTTCTG | 59.9 | 0.37 |
| mm4_intergenic_C130071C03Rik/Mir9-2|Gm17750_chr13_83955417_F | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intergenic_C130071C03Rik/Mir9-2|Gm17750_chr13_83955417_R | Primer3: not found at this Tm | N.d. | 0.35 |
| mm3_intron_Btbd16_chr7_130797859_F | TCGTCGGCAGCGTCTGCTTCTGGCTTTGACCCTT | 59.8 | 0.32 |
| mm3_intron_Btbd16_chr7_130797859_R | GTCTCGTGGGCTCGGAGGAGGACACATGCACAGTG | 59.9 | 0.32 |
| mm4_intergenic_Kctd3|Kcnk2_chr1_189088954_F | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intergenic_Kctd3|Kcnk2_chr1_189088954_R | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intergenic_Gm17054|n-R5s37_chr16_61145430_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intergenic_Gm17054|n-R5s37_chr16_61145430_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm3_intron_Aldh1l1_chr6_90515292_F | TCGTCGGCAGCGTCGTCAGTTCTGTGGGATCCCG | 60.1 | 0.28 |
| mm3_intron_Aldh1l1_chr6_90515292_R | GTCTCGTGGGCTCGGCGCCATCTGCTTAGAGAGGG | 59.9 | 0.28 |
| mm4_intron_Arhgap29_chr3_121988164_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intron_Arhgap29_chr3_121988164_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Rhobtb2|Pebp4_chr14_69837090_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Rhobtb2|Pebp4_chr14_69837090_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intron_Arhgap26_chr18_38945190_F | TCGTCGGCAGCGTCTGGGAGAGAAGTGGGGACAA | 60.1 | 0.26 |
| mm4_intron_Arhgap26_chr18_38945190_R | GTCTCGTGGGCTCGGCCAGTGCAGGATGCTACACA | 60.0 | 0.26 |
| mm4_intergenic_Il23a|Gm23241_chr10_128298204_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_Il23a|Gm23241_chr10_128298204_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_Zfp652|Phospho1_chr11_95780682_F | TCGTCGGCAGCGTCACACCCAGCAGAAATCTTGGA | 59.5 | 0.25 |
| mm4_intergenic_Zfp652|Phospho1_chr11_95780682_R | GTCTCGTGGGCTCGGTGCATAGAACGTCGGACACA | 59.4 | 0.25 |
| mm4_exon_Map3k3_chr11_106123799_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_exon_Map3k3_chr11_106123799_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm3_intergenic_Gm25577|Gm22537_chr12_118542877_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm3_intergenic_Gm25577|Gm22537_chr12_118542877_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Gm22674|Cdyl_chr13_35541955_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Gm22674|Cdyl_chr13_35541955_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Ighv6-3|Ighv6-4_chr12_114402154_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Ighv6-3|Ighv6-4_chr12_114402154_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Lipg|Rpl17_chr18_74981727_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Lipg|Rpl17_chr18_74981727_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Gm12421|Bnc2_chr4_84440489_F | TCGTCGGCAGCGTCTGTCTCCTCTCTGTTCTGCTTG | 59.7 | 0.22 |
| mm4_intergenic_Gm12421|Bnc2_chr4_84440489_R | GTCTCGTGGGCTCGGTGGCAGCCTCTGGTAATGAC | 59.7 | 0.22 |
| mm4_intron_Slco5a1_chr1_12948943_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Slco5a1_chr1_12948943_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Ttc28|TTC28-AS1_1_chr5_111323139_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Ttc28|TTC28-AS1_1_chr5_111323139_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Rtn4rl1_chr11_75228019_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Rtn4rl1_chr11_75228019_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_St3gal3_chr4_118096381_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_St3gal3_chr4_118096381_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_Il4ra_chr7_125560476_F | TCGTCGGCAGCGTCGAGTCCTCAGTGGCTCCCTA | 60.0 | 0.18 |
| mm4_intron_Il4ra_chr7_125560476_R | GTCTCGTGGGCTCGGCGGGTGTGGTTGGCAATAAC | 59.7 | 0.18 |
| mm4_intergenic_Cdyl2|Gm10620_chr8_116754805_F | TCGTCGGCAGCGTCAGAAGCACTTCAGAGATGGTTCT | 59.4 | 0.18 |
| mm4_intergenic_Cdyl2|Gm10620_chr8_116754805_R | GTCTCGTGGGCTCGGAGGACAGCCTTGTTTCCCAG | 59.8 | 0.18 |
| mm4_intron_Susd4_chr1_182834702_F | TCGTCGGCAGCGTCGTTGAGACACAGCACTCCCA | 59.8 | 0.18 |
| mm4_intron_Susd4_chr1_182834702_R | GTCTCGTGGGCTCGGGTCGGTTTGCCCAAGCTTTT | 59.8 | 0.18 |
| mm4_intron_Prkch_chr12_73678243_F | TCGTCGGCAGCGTCGCCGTGTGAAGCAATCCATAC | 59.9 | 0.18 |
| mm4_intron_Prkch_chr12_73678243_R | GTCTCGTGGGCTCGGCCTCATCCACTTCCATCAGGA | 58.8 | 0.18 |
| mm3_intergenic_Spock1|Klhl3_chr13_57950882_F | TCGTCGGCAGCGTCGTCACTCAGGACCGACACAT | 59.3 | 0.17 |
| mm3_intergenic_Spock1|Klhl3_chr13_57950882_R | GTCTCGTGGGCTCGGCTGCCTGCAGTTTCTTTGCT | 59.3 | 0.17 |
| mm4_intron_Trpv2_chr11_62591119_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Trpv2_chr11_62591119_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm25136|Grin2b/Gm14329_chr6_135613957_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm25136|Grin2b/Gm14329_chr6_135613957_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm8730|Cdh5_chr8_103737422_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm8730|Cdh5_chr8_103737422_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm12066|Gm12068_chr11_24349131_F | TCGTCGGCAGCGTCGCGTATCAGAGGCTGAGTGT | 59.5 | 0.17 |
| mm4_intergenic_Gm12066|Gm12068_chr11_24349131_R | GTCTCGTGGGCTCGGTGTCATTAGCAGGCACCCTC | 59.7 | 0.17 |
| mm4_exon_Stab2_chr10_86841449_F | TCGTCGGCAGCGTCTGTCCCCTTACCCATGACGA | 60.2 | 0.17 |
| mm4_exon_Stab2_chr10_86841449_R | GTCTCGTGGGCTCGGGCTCCAGCTGTCACTTGGAT | 60.0 | 0.17 |
| mm4_intron_Naa50_chr16_44143716_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Naa50_chr16_44143716_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_exon_Meis2_chr2_116049122_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_exon_Meis2_chr2_116049122_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Cpeb2|C1qtnf7_chr5_43437375_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Cpeb2|C1qtnf7_chr5_43437375_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Ppm1h|Mirlet7i_chr10_122966526_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Ppm1h|Mirlet7i_chr10_122966526_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm3_intron_Kcnd3_chr3_105552510_F | TCGTCGGCAGCGTCCAACTTCCCAAGTCCCTGCA | 60.1 | 0.15 |
| mm3_intron_Kcnd3_chr3_105552510_R | GTCTCGTGGGCTCGGAGGAAAGATGTCAGCCAGGG | 59.3 | 0.15 |
| mm4_intergenic_Nkd2|Trip13_chr13_73882145_F | TCGTCGGCAGCGTCTGACTGCACAAAGCTGACCA | 60.1 | 0.14 |
| mm4_intergenic_Nkd2|Trip13_chr13_73882145_R | GTCTCGTGGGCTCGGCCAGTCAGGGACTCACACAC | 59.9 | 0.14 |
| mm4_intergenic_Gm26794/Kcnj3|Kcnj3_chr2_55439040_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm26794/Kcnj3|Kcnj3_chr2_55439040_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Naif1|Gm13412_chr2_32517280_F | TCGTCGGCAGCGTCCTGGGTACTGCAAGCCTCTC | 60.1 | 0.13 |
| mm4_intergenic_Naif1|Gm13412_chr2_32517280_R | GTCTCGTGGGCTCGGGGCGTTCCTCAGACTTAGCA | 59.7 | 0.13 |
| mm4_intergenic_Akap6|Npas3_chr12_53246161_F | TCGTCGGCAGCGTCACATTGCTGGCCTTAACCCA | 59.8 | 0.13 |
| mm4_intergenic_Akap6|Npas3_chr12_53246161_R | GTCTCGTGGGCTCGGGCTCTGTGCAGGCTTTCTAC | 58.9 | 0.13 |
| mm3_intron_Msra_chr14_64234121_F | TCGTCGGCAGCGTCTCATCCCCAGTCTCAGTCCC | 60.3 | 0.13 |
| mm3_intron_Msra_chr14_64234121_R | GTCTCGTGGGCTCGGGGGGAAAATGAAAATGGAGTTGAGT | 59.9 | 0.13 |
| mm4_intergenic_Scaf4|Hunk_chr16_90292827_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Scaf4|Hunk_chr16_90292827_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm3_exon_Gm5205_chr14_40557804_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm3_exon_Gm5205_chr14_40557804_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_exon_Gdf5/Gm15557_chr2_155941237_F | TCGTCGGCAGCGTCAAGTCACCAGGCACAAAGGT | 59.7 | 0.13 |
| mm4_exon_Gdf5/Gm15557_chr2_155941237_R | GTCTCGTGGGCTCGGGTTCACCATCTCTCCTGCCC | 60.1 | 0.13 |
| mm4_intergenic_Fam155a|n-R5s95_chr8_9385173_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Fam155a|n-R5s95_chr8_9385173_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Mir139|Pde2a_chr7_101478563_F | TCGTCGGCAGCGTCTTGACAGCCTGCCTATGTGG | 60.0 | 0.12 |
| mm4_intergenic_Mir139|Pde2a_chr7_101478563_R | GTCTCGTGGGCTCGGGCCAGCCTCAAACAGTCCTA | 59.6 | 0.12 |
| mm4_intron_Vdr_chr15_97904780_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Vdr_chr15_97904780_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Rassf9|Alx1_chr10_102629837_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Rassf9|Alx1_chr10_102629837_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3_intergenic_Tsen54|Llgl2_chr11_115823917_F | TCGTCGGCAGCGTCCACTACTGGGGCCACGATTG | 60.7 | 0.10 |
| mm3_intergenic_Tsen54|Llgl2_chr11_115823917_R | GTCTCGTGGGCTCGGTTACATACAGGGCGAGCCTG | 59.5 | 0.10 |
| mm4_intergenic_Col4a3|n-R5s213_chr1_82607017_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Col4a3|n-R5s213_chr1_82607017_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm3_intron_Wisp2_chr2_163828038_F | TCGTCGGCAGCGTCGGCAGTGTGGACAGAGAACA | 59.8 | 0.10 |
| mm3_intron_Wisp2_chr2_163828038_R | GTCTCGTGGGCTCGGTACCTTTCTGGAGGGCTGGA | 59.8 | 0.10 |
| mm4_intergenic_Gm26119|Fzd8_chr18_8819217_F | TCGTCGGCAGCGTCCTGTCCCCAAGAAGCCTAGC | 60.1 | 0.09 |
| mm4_intergenic_Gm26119|Fzd8_chr18_8819217_R | GTCTCGTGGGCTCGGTAGGAGCTTTGTGTGCCACT | 59.2 | 0.09 |
| mm4_intergenic_Gm11204|Utp6_chr11_79917251_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm11204|Utp6_chr11_79917251_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Fhit_chr14_10453317_F | TCGTCGGCAGCGTCAAGGTCTGCCTCCAACACTG | 59.8 | 0.09 |
| mm4_intron_Fhit_chr14_10453317_R | GTCTCGTGGGCTCGGCCTGTGGATCAGTCCTCTGC | 59.8 | 0.09 |
| mm4_intron_Pdgfrl_chr8_40986644_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Pdgfrl_chr8_40986644_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Sipa1l3_chr7_29324246_F | TCGTCGGCAGCGTCTTCAGCATCACCTCCAGCTG | 60.0 | 0.09 |
| mm4_exon_Sipa1l3_chr7_29324246_R | GTCTCGTGGGCTCGGCTGAGGTGTCAGGCATCAGG | 60.1 | 0.09 |
| mm3_intergenic_Mex3b|Gm22934_chr7_82946196_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intergenic_Mex3b|Gm22934_chr7_82946196_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Pdzd2|Gm24302_chr15_12638569_F | TCGTCGGCAGCGTCTGGTGCATCTCAAGATCGGG | 59.8 | 0.08 |
| mm4_intergenic_Pdzd2|Gm24302_chr15_12638569_R | GTCTCGTGGGCTCGGGCTTCATTGCTCCTGGGAGT | 60.0 | 0.08 |
| mm3_intergenic_Gm5084|Gm24999_chr13_60226808_F | TCGTCGGCAGCGTCGTGGTCGTCCAAGGTCTTCC | 60.3 | 0.08 |
| mm3_intergenic_Gm5084|Gm24999_chr13_60226808_R | GTCTCGTGGGCTCGGGAAACTGTCTCCAGCAGCCA | 60.2 | 0.08 |
| mm4_intergenic_D630014O11Rik|Galnt14_chr17_73655271_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_D630014O11Rik|Galnt14_chr17_73655271_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_4732465J04Rik|Gm24433_chr10_95827946_F | TCGTCGGCAGCGTCACACAACAGCATCCTCATGACA | 60.2 | 0.07 |
| mm4_intergenic_4732465J04Rik|Gm24433_chr10_95827946_R | GTCTCGTGGGCTCGGAGCTGTAGAAGGCAGGGAGA | 59.9 | 0.07 |
| mm4_intron_Ispd_chr12_36501357_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Ispd_chr12_36501357_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intron_Sufu_chr19_46477781_F | TCGTCGGCAGCGTCAGTTGCTGTGCTAGGAGCTG | 60.0 | 0.07 |
| mm3_intron_Sufu_chr19_46477781_R | GTCTCGTGGGCTCGGCTTGGTGATCAAGGGTGCGT | 60.6 | 0.07 |
| mm4_exon_Tm9sf4_chr2_153200367_F | TCGTCGGCAGCGTCTGGGATTCTCAGTCCCCACT | 59.8 | 0.06 |
| mm4_exon_Tm9sf4_chr2_153200367_R | GTCTCGTGGGCTCGGCTCCTGTGGGGAAAGCAGAG | 60.0 | 0.06 |
| mm4_intron_Pou2f3_chr9_43134214_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Pou2f3_chr9_43134214_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Cdhr3_chr12_33074615_F | TCGTCGGCAGCGTCTAGATCACGTTGCCTCCAGC | 59.8 | 0.06 |
| mm4_intron_Cdhr3_chr12_33074615_R | GTCTCGTGGGCTCGGGTATTTCCCCACCTGCGGAA | 60.0 | 0.06 |
| mm4_intergenic_Mc3r|Gm14275_chr2_172282097_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Mc3r|Gm14275_chr2_172282097_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Smap1|1110058L19Rik_chr1_23994147_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Smap1|1110058L19Rik_chr1_23994147_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gpc1|Ankmy1_chr1_92865929_F | TCGTCGGCAGCGTCCTGAAGTGGGGCCTAAGGAC | 59.7 | 0.05 |
| mm4_intergenic_Gpc1|Ankmy1_chr1_92865929_R | GTCTCGTGGGCTCGGGTGGGCAGAGACTGACTGAC | 60.0 | 0.05 |
| mm4_intron_Pip4k2a_chr2_18908016_F | TCGTCGGCAGCGTCGTCAGAAAAGTCCTCATCTGCC | 58.9 | 0.05 |
| mm4_intron_Pip4k2a_chr2_18908016_R | GTCTCGTGGGCTCGGTCCCTGTCATGTTGATGCCT | 59.0 | 0.05 |
| mm4_intron_Tmprss3_chr17_31183399_F | TCGTCGGCAGCGTCTGTTTGCCTAGCACCTGATGT | 59.9 | 0.05 |
| mm4_intron_Tmprss3_chr17_31183399_R | GTCTCGTGGGCTCGGAGAGGCCTTGCTGCAATGAA | 60.2 | 0.05 |
| mm3_intergenic_Rab17|Lrrfip1_chr1_90972879_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_Rab17|Lrrfip1_chr1_90972879_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_RP23-175E16.1|RP24-225I21.1/Kirrel3_chr9_34443998_F | TCGTCGGCAGCGTCGCTTCTCTGACAGGATGCCA | 59.7 | 0.04 |
| mm4_intergenic_RP23-175E16.1|RP24-225I21.1/Kirrel3_chr9_34443998_R | GTCTCGTGGGCTCGGTCGTCTACTAGTACTGCTTAGGGA | 59.5 | 0.04 |
| mm4_intergenic_Bcas3|8430403D17Rik_chr11_85689447_F | TCGTCGGCAGCGTCAGGAAATGATGGGTGGCACC | 60.3 | 0.04 |
| mm4_intergenic_Bcas3|8430403D17Rik_chr11_85689447_R | GTCTCGTGGGCTCGGCTCTGTCTGACAAGCCTCCG | 60.1 | 0.04 |
| mm4_intergenic_mmu-mir-7239|Cyfip2_chr11_46181852_F | TCGTCGGCAGCGTCAGAAGTGAGTGAGAACATCAGAGA | 58.9 | 0.04 |
| mm4_intergenic_mmu-mir-7239|Cyfip2_chr11_46181852_R | GTCTCGTGGGCTCGGCACAGCCAGATCGTGATGGT | 60.1 | 0.04 |
| mm4_exon_Stx1b_chr7_127806722_F | TCGTCGGCAGCGTCAAGATGTGCAGCATGGTCGC | 61.6 | 0.04 |
| mm4_exon_Stx1b_chr7_127806722_R | GTCTCGTGGGCTCGGACATCTGGAGTGGGAGGAGA | 59.2 | 0.04 |
| mm4_exon_1110034G24Rik_chr2_132750588_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_1110034G24Rik_chr2_132750588_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Cmklr1|1700069L16Rik_chr5_113684511_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Cmklr1|1700069L16Rik_chr5_113684511_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intron_Tmem182_chr1_40847903_F | TCGTCGGCAGCGTCTCTGCCAGCCTTTCAGACAG | 59.9 | 0.04 |
| mm3_intron_Tmem182_chr1_40847903_R | GTCTCGTGGGCTCGGTGTCCGTCAATACCAAACACG | 58.8 | 0.04 |
| mm4_intron_Lonrf1_chr8_36225388_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Lonrf1_chr8_36225388_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Gm22684|Anxa1_chr19_20094090_F | TCGTCGGCAGCGTCACTCTCAATTCGTTAAGGCCA | 57.3 | 0.03 |
| mm3_intergenic_Gm22684|Anxa1_chr19_20094090_R | GTCTCGTGGGCTCGGTGAGAAGCAGAGCAATTGTTTCA | 59.1 | 0.03 |
| mm4_intergenic_B4galt5|Slc9a8_chr2_167357112_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_B4galt5|Slc9a8_chr2_167357112_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Sh3bp4_chr1_89137221_F | TCGTCGGCAGCGTCGCTCTCTGTTCCTTGTGCCA | 60.2 | 0.03 |
| mm4_intron_Sh3bp4_chr1_89137221_R | GTCTCGTGGGCTCGGCCTCAGACCGTGCAGTACAT | 59.4 | 0.03 |
| mm4_intron_Kcnj3_chr2_55447478_F | TCGTCGGCAGCGTCGGGCATTTTGTGATCAGCCTG | 60.1 | 0.03 |
| mm4_intron_Kcnj3_chr2_55447478_R | GTCTCGTGGGCTCGGACACACAAGCACACAGGACA | 60.0 | 0.03 |
| mm3_intergenic_Rxrb|Col11a2_chr17_34039130_F | TCGTCGGCAGCGTCGATTACGAGTGGGCGTGGAA | 60.1 | 0.03 |
| mm3_intergenic_Rxrb|Col11a2_chr17_34039130_R | GTCTCGTGGGCTCGGTAGTCCTTTCACACCACGGC | 59.9 | 0.03 |
| mm4_intron_Brf1_chr12_112967602_F | TCGTCGGCAGCGTCAAACTGACAGTGGGACAGCC | 60.1 | 0.03 |
| mm4_intron_Brf1_chr12_112967602_R | GTCTCGTGGGCTCGGGCAGTGACCAGGACCTGTTA | 59.3 | 0.03 |
| mm4_intergenic_CT030233.1|Gm20675_chr14_67157567_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_CT030233.1|Gm20675_chr14_67157567_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Cpq_chr15_33308099_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Cpq_chr15_33308099_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Gpr179_chr11_97347466_F | TCGTCGGCAGCGTCCAGAGATAGCGGCCAAGGAC | 60.2 | 0.02 |
| mm4_exon_Gpr179_chr11_97347466_R | GTCTCGTGGGCTCGGCCACAGCACGGAAAGCTTTC | 60.0 | 0.02 |
| mm3_intergenic_Gm24371|BC024582_chr4_32540355_F | TCGTCGGCAGCGTCACAGACTGTGTCAGGAACGT | 58.8 | 0.02 |
| mm3_intergenic_Gm24371|BC024582_chr4_32540355_R | GTCTCGTGGGCTCGGGGTCCTTGCCCATGTATGCT | 60.1 | 0.02 |
| mm4_intergenic_Nt5m|Gm12714_chr11_59882093_F | TCGTCGGCAGCGTCCAGGAGGCTTTTCTTCGGGA | 59.6 | 0.02 |
| mm4_intergenic_Nt5m|Gm12714_chr11_59882093_R | GTCTCGTGGGCTCGGCACTCGCTTCAGCCCTGTAA | 60.0 | 0.02 |
| mm4_intron_Sdk2_chr11_113856508_F | TCGTCGGCAGCGTCAGGAACGACCCAAGCTTGTT | 59.8 | 0.02 |
| mm4_intron_Sdk2_chr11_113856508_R | GTCTCGTGGGCTCGGGGTCTGGCTCAGCTTCTCTC | 59.8 | 0.02 |
| mm4_exon_Susd4_chr1_182764166_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Susd4_chr1_182764166_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intron_Ddx41_chr13_55533905_F | TCGTCGGCAGCGTCAGAACTGTGTGAGGCCCATG | 59.9 | 0.02 |
| mm3_intron_Ddx41_chr13_55533905_R | GTCTCGTGGGCTCGGCGTACTGATGGTCTCGGTGG | 59.8 | 0.02 |
| mm4_intergenic_Olfr1219|Olfr1220_chr2_89093619_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Olfr1219|Olfr1220_chr2_89093619_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Rit1_chr3_88730351_F | TCGTCGGCAGCGTCCAGGCCTTGTCCAGTGTTCT | 59.8 | 0.02 |
| mm4_exon_Rit1_chr3_88730351_R | GTCTCGTGGGCTCGGCTCAGGGCAAGGGAACACAA | 60.1 | 0.02 |
| mm4_intron_Antxr1_chr6_87158191_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Antxr1_chr6_87158191_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Efcab6_chr15_83921805_F | TCGTCGGCAGCGTCTGACAAGCTTTGCCATGACC | 59.0 | 0.01 |
| mm4_intron_Efcab6_chr15_83921805_R | GTCTCGTGGGCTCGGCTACAGAGGGGTGGCAGAGA | 60.3 | 0.01 |
| mm4_intron_Plekha7_chr7_116288632_F | TCGTCGGCAGCGTCGGGATGACTACCTGGGGACT | 60.0 | 0.01 |
| mm4_intron_Plekha7_chr7_116288632_R | GTCTCGTGGGCTCGGGCCAGCAACTTCCTCTCACT | 59.9 | 0.01 |
| mm4_intron_Csmd2_chr4_128553287_F | TCGTCGGCAGCGTCGTGGGAATGGGTGAGAGAGC | 60.1 | 0.01 |
| mm4_intron_Csmd2_chr4_128553287_R | GTCTCGTGGGCTCGGCGGAAGAACGTGGTGAGGAA | 59.9 | 0.01 |
| mm4_intron_Tiam1_chr16_89972189_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Tiam1_chr16_89972189_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm12708|Gm830_chr4_95404939_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm12708|Gm830_chr4_95404939_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_B4galt5_chr2_167330783_F | TCGTCGGCAGCGTCCACAGCACCTTGAGACTGGT | 59.8 | 0.01 |
| mm4_intron_B4galt5_chr2_167330783_R | GTCTCGTGGGCTCGGGTGCACCTGCTACCCTCAAT | 60.0 | 0.01 |
| mm4_exon_Tnfaip2_chr12_111445097_F | TCGTCGGCAGCGTCCCGAGGCTGAATCAGAGACC | 59.8 | 0.01 |
| mm4_exon_Tnfaip2_chr12_111445097_R | GTCTCGTGGGCTCGGTGAACATGTTGGCCAGTCCT | 59.5 | 0.01 |
| mm4_exon_Otop1_chr5_38300322_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Otop1_chr5_38300322_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Bcar3_chr3_122420409_F | TCGTCGGCAGCGTCGTGACCAAAGTGTGTGTGGC | 59.8 | 0.01 |
| mm4_intron_Bcar3_chr3_122420409_R | GTCTCGTGGGCTCGGCGGAGCCCAAGAGTAGATGG | 59.6 | 0.01 |
| mm3_intergenic_Zfp42|Gm22986_chr8_43330715_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Zfp42|Gm22986_chr8_43330715_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Lpcat2|Slc6a2_chr8_92933860_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Lpcat2|Slc6a2_chr8_92933860_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Itga2_chr13_114838529_F | TCGTCGGCAGCGTCGGTTCACGGCAGTTCCAGTT | 60.8 | 0.00 |
| mm4_intron_Itga2_chr13_114838529_R | GTCTCGTGGGCTCGGGCTGCTTTTCTGCCTCTGGA | 60.6 | 0.00 |
| mm4_intron_Nfat5_chr8_107294012_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Nfat5_chr8_107294012_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_exon_Card11_chr5_140882027_F | TCGTCGGCAGCGTCCAGGAGCTGGCCAAAGAAGA | 59.9 | 0.00 |
| mm3_exon_Card11_chr5_140882027_R | GTCTCGTGGGCTCGGGCCCGAAGAGATGCTTTCGA | 60.4 | 0.00 |
| mm4_intron_Ptrf_chr11_100965308_F | TCGTCGGCAGCGTCCTTGGGAGAGGGACAGTTGG | 59.6 | 0.00 |
| mm4_intron_Ptrf_chr11_100965308_R | GTCTCGTGGGCTCGGCACCCACCTCCCTGATGTTC | 60.0 | 0.00 |
| mm4_intron_Lrrc48_chr11_60382341_F | TCGTCGGCAGCGTCCCATTCGCACCTTCTGAGGA | 59.7 | 0.00 |
| mm4_intron_Lrrc48_chr11_60382341_R | GTCTCGTGGGCTCGGCAGCTTCTTGTGGGCTGAGA | 59.9 | 0.00 |
| mm4_intron_Wwox_chr8_115011141_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Wwox_chr8_115011141_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Auts2_chr5_131670614_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Auts2_chr5_131670614_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Mcmbp_chr7_128697499_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Mcmbp_chr7_128697499_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Sept9_chr11_117201149_F | TCGTCGGCAGCGTCATGTGCTCAGGTTTGGCACT | 60.1 | 0.00 |
| mm4_intron_Sept9_chr11_117201149_R | GTCTCGTGGGCTCGGGGAGAAGAAAGCACAGCCCT | 59.9 | 0.00 |
| mm4_intergenic_Gm11680|BC006965_chr11_112633335_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm11680|BC006965_chr11_112633335_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_2410088K16Rik|Sh3bp4_chr1_89014499_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_2410088K16Rik|Sh3bp4_chr1_89014499_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313824 |
AGGGAGCCTGGGATTAGGAGCTTACTTGGGGGTTTTCCCCCTCCCCCCCTCTGGAGAGTCCGGGGATGGAGAGCCGAAAG GACATGGT |
| mm4_intron_Capn8_chr1_182627895 |
GAAGACAAAGCACGCCACTGACATTAGCCCCATTTCTATGTGTTCCAGAGTTTCTTTGGAGATGGCAATACAGCCTCTCT AGAGTGCATCCTTGGTCTCTCAAGAGAGGAGTCGATGGGGACGGAACTGTCAATG |
| mm4_intergenic_Gyg|Cpa3_chr3_20191587 |
TCCAATTCTATTCCCTTCTCAGCATCTATTAACTAGCTATAGATCCCCAGAATCTCCAGAAAGGGGAAGCGCTTCCNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNGATGTTCTGTGTTGATCTGCAGCCCAGGAATC |
| mm4_intron_Xrcc5_chr1_72310267 |
ACCCCTCTGTCTATGTGGCTTTTAGGAAATAGTAGAGCCATTCACAGGCTCTCCAGAGTGGAACTTTACGTGTTTTTGAA ACTCAACACCGTCTTAAACATGTTCTCTCCCTTTATCCTTTTTACGTAGCAGGACAGGGA |
| mm4_intergenic_Plk2|Actbl2_chr13_110422226 |
GGTCCCCTCTGTCCCTGTATCTTAACCATTCTGGAGAGTGCATGGATGTTCTGGGTATTTTGTGACCTTTGACACAGCAG TGTATACAAATGAATTCAAAGAGGAAGTACAGTGGCATCTTAGTTAGGTCCTGGGGCTGA |
| mm4_intergenic_Atp6v1b2|Lzts1_chr8_69115924 |
AGCAACACAGGTCACACACACAGCTCTAGGGAGGCCAGGCCCCGCTCTTGAGTGTCCGTGGCTGGGCACTCTGGCTCCCA GAACCAGATAGTGCCTTCTGTCAAGCTCTGCATCAGCT |
| mm4_intron_Arhgef3_chr14_27399347 |
GCTCCAACCCAGCTTCTCATCTTGACTTGTTACTCCTCCCCGTTAGTGGCCTCTTTTGAGAGTCCTGGGTTGACATTCCC ACACGGTTTTTCTACCAGAAAGTCAGAGCCCAGCA |
| mm3_intron_Btbd16_chr7_130797859 |
TGCTTCTGGCTTTGACCCTTAGGTGTGTAATCTAGCCGCACCATCTCATGGTCTGACACAGCCCCACAGGGACCTTCCTC GGACTCTCCACAGAGGTGTAGCCACACTGTGCATGTGTCCTCCT |
| mm3_intron_Aldh1l1_chr6_90515292 |
GTCAGTTCTGTGGGATCCCGGGAGGTCTCTGATACATGGTGACTAGGAGGCCACTTTGGAGAGCCCTGGGATGAGACCCT CCCTCTCTAAGCAGATGGCG |
| mm4_intron_Arhgap26_chr18_38945190 |
TGGGAGAGAAGTGGGGACAAAGCCATACAGCATGCCAGGAACCTCCAGAGTGGCCCCAGCAGAGGGACCTGGACTGGGAG GAAGGGATAAAGAAGCTTGGCTGCCCCCTGGAGTTGTGTAGCATCCTGCACTGG |
| mm4_intergenic_Zfp652|Phospho1_chr11_95780682 |
ACACCCAGCAGAAATCTTGGATCTTGAGGGAGAAACATGGGTAGAAACAGCTGGACTCCCTCCGTGGACACTCCAGAGAG GAATGTGCCTTTGGTGGCCAGCGTGGTGCAACTGTGTCCGACGTTCTATGCA |
| mm4_intergenic_Gm12421|Bnc2_chr4_84440489 |
TGTCTCCTCTCTGTTCTGCTTGACTCTCATTCAAATGCAAGGTGTTTCAGTTCTCCTCACTGGAGAGTTCAGGAATGCAA CATGAAGAAGCTCTGAAGTGCAAGAGTCATTACCAGAGGCTGCCA |
| mm4_intron_Il4ra_chr7_125560476 |
GAGTCCTCAGTGGCTCCCTATTGCCCCCAGACAGCCCCAGTCTCTCCAGACTGGTAGATGGTGCTGCCACCTGGACTTGC CAACCTCTCTGTTATTGCCAACCACACCCG |
| mm4_intergenic_Cdyl2|Gm10620_chr8_116754805 |
AGAAGCACTTCAGAGATGGTTCTATCAGGGAACATTCTGTGCTATAAGAACTTGGCCACTCTTGAGAGTCAGGCGCTGGA GAGTGTCCCTTTGTGAACTGGGAAACAAGGCTGTCCT |
| mm4_intron_Susd4_chr1_182834702 |
GTTGAGACACAGCACTCCCAATCAGACCCTGACTCTCCAAAGGGGGAAGGGGGGGGTCCAGACCTGCATTCTGGTGTTGA ATGGTAAGCAATGTTTGGAAAAGCTTGGGCAAACCGAC |
| mm4_intron_Prkch_chr12_73678243 |
GCCGTGTGAAGCAATCCATACCTTTGTCAACAGAGGAATTTTATCAGAAGAAAACATGGAAAGTTTTTGTGTGACAGTAT TGGACTCTTTCCCTTCTGGTGAGTCAGGGCATGATCCTGATGGAAGTGGATGAGG |
| mm3_intergenic_Spock1|Klhl3_chr13_57950882 |
GTCACTCAGGACCGACACATAGTATCCTGATTCTCTTTATAAATGCCTACCCGGCCCATGCTCATTGGGAACAACCACTC TGGTGCGTCCGGTGATGTTTTGCTTGAAAGCTTCAAATGTAGCAAAGAAACTGCAGGCAG |
| mm4_intergenic_Gm12066|Gm12068_chr11_24349131 |
GCGTATCAGAGGCTGAGTGTGATCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGCCCTGGGA TCTTCCCCAGAGTCTCCAGAAAGGAGGCCACAGAGAGGGTGCCTGCTAATGACA |
| mm4_exon_Stab2_chr10_86841449 |
TGTCCCCTTACCCATGACGACAACCCCAGACTAACCAGAGGGGTGAGGTATGGCTTCTGAGTTATCATACTTTAAAACAA ATGATCCAAGTGACAGCTGGAGC |
| mm3_intron_Kcnd3_chr3_105552510 |
CAACTTCCCAAGTCCCTGCATAAATCATTTTCCAAGAGCCTATCTAGAGGGCGCCTTCTGCATCCTGGCTGAGCCTGAGC TTCCCCAGACACTCCAGAGCAGTTCCTTCTTGCATGTAAGCCCTGGCTGACATCTTTCCT |
| mm4_intergenic_Nkd2|Trip13_chr13_73882145 |
TGACTGCACAAAGCTGACCAAATATCCTCCATGGAGTCTCCAGAGAGGAGAATAATGGAGCCTTTCTTGGAAATTCTTGT GTGTGTGAGTCCCTGACTGG |
| mm4_intergenic_Naif1|Gm13412_chr2_32517280 |
CTGGGTACTGCAAGCCTCTCTAAGAGTTTCTAAAAAGGGGGCCAGCTATCCCCCTCTGGAGAGGCCAGAAATGTCCTGAT GCTAAGTCTGAGGAACGCC |
| mm4_intergenic_Akap6|Npas3_chr12_53246161 |
ACATTGCTGGCCTTAACCCAGGTAGAAATGCTCTAAAACATCCCTGCCCTCTCCAGAATGGGTAGGAAGTCTCTGTCTCA GTAGAAAGCCTGCACAGAGC |
| mm3_intron_Msra_chr14_64234121 |
TCATCCCCAGTCTCAGTCCCCGGTTATAAAAGCATAGTTATCCTCCCTGGAGATTCCCGGGATGAAGACTTCTGTGTCTT TCATATGAGAACTCAACTCCATTTTCATTTTCCCC |
| mm4_exon_Gdf5/Gm15557_chr2_155941237 |
AAGTCACCAGGCACAAAGGTGATTCAAGGAGGAGACAGCTCTCCCTTAAAGAGCTTAGGATATAGCTACAACTTCTCTGG AGAGTCTGGAGAGAAATGAAGAGGCCCCGGGCAGGAGAGATGGTGAAC |
| mm4_intergenic_Mir139|Pde2a_chr7_101478563 |
TTGACAGCCTGCCTATGTGGCACATGGTTGGGAGGGGGCGAGGGAGGACAGACAAACAAGTGGGTAAAAATGGCTGTTTT TCCCTCCCTTGAGAGTCCTGGGGTGCTAGGACTGTTTGAGGCTGGC |
| mm3_intergenic_Tsen54|Llgl2_chr11_115823917 |
CACTACTGGGGCCACGATTGGTTGGAGAGCGGCCCCGCTCTGGAGACTCCCAGGATGCGCTCCTGCCCCGTCGGCTCGGC CTAGGAGGCGGGGCCGGGGCGGGGCGAGGGCTCGGCAGGCTCGCCCTGTATGTAA |
| mm3_intron_Wisp2_chr2_163828038 |
GGCAGTGTGGACAGAGAACAGTCCTCTCTGGAGAATAAGGGGATGTTAGGAAAAGTACTCTGGACTCCAGCCCTCCAGAA AGGTA |
| mm4_intergenic_Gm26119|Fzd8_chr18_8819217 |
CTGTCCCCAAGAAGCCTAGCATGGCTCTCCAATGTTGGTACAGCCTCTTCTCACCACTGTGGAGAGTTCGCAGATGCCTT ACAGTGGCACACAAAGCTCCTA |
| mm4_intron_Fhit_chr14_10453317 |
AAGGTCTGCCTCCAACACTGCTGTCCATCCCAGCAGCCTCCAGAGTGGACTTTTGTACTTTAGGAATTAGGGGTACCTGT GTTTTCCAAGCAGAGGACTGATCCACAGG |
| mm4_exon_Sipa1l3_chr7_29324246 |
TTCAGCATCACCTCCAGCTGGTACACCTTGCCTGTCAGATCCAGGGGTGACTCCTCGCTGGAGAGACAAGGGATGGGGTC AGTCCTGATGCCTGACACCTCAG |
| mm4_intergenic_Pdzd2|Gm24302_chr15_12638569 |
TGGTGCATCTCAAGATCGGGGTTATTCTAAGTGCTATTTCCAGGCCCGGGAACCACCGCCCTGGAGAGTCAGGGCTTGGG AGGATAAAGACTGGAGCAGTTCACCCACACTATATACTCCCAGGAGCAATGAAGC |
| mm3_intergenic_Gm5084|Gm24999_chr13_60226808 |
GTGGTCGTCCAAGGTCTTCCAAGGCTGCTCTGGAGAGGCTGTGGATGAAAACAAGAGAGAGCTANNNNNNNNNNNNNNNN NNNNNNNNNNACAAAAGAAAGCCGTGGGTACCGTGTGCTGGCTGCTGGAGACAGTTTC |
| mm4_intergenic_4732465J04Rik|Gm24433_chr10_95827946 |
ACACAACAGCATCCTCATGACAAGTATGACCACTGAAGGCCTCTTTGGACAGTCTAGGGATGCTTTCTCTCTCTTCTGTC TGTCTCCCTGCCTTCTACAGCT |
| mm3_intron_Sufu_chr19_46477781 |
AGTTGCTGTGCTAGGAGCTGCTTAATCAAAGGCCTGTTATGGGCTCATTAGTGTGGTCCACCACGGGCCACCCCTCAGGA GAGTCCTGGGAAGGCTGGCTTAGGTCCCGCCCTTCAGGCACGCACCCTTGATCACCAAG |
| mm4_exon_Tm9sf4_chr2_153200367 |
TGGGATTCTCAGTCCCCACTGCCTCCCTGGAGGCTCCTGGGATGCTGAGGTTCCATCTTCCCTGGAAGCTCACATGGCCC CATCTGCACTCTGCTTTCCCCACAGGAG |
| mm4_intron_Cdhr3_chr12_33074615 |
TAGATCACGTTGCCTCCAGCTGCAAAACCTATTAGCATCCCCAGAGTCTCCAAGGAGGCCCTCGGCAATGAGAGGGATGC AGGGAGGGTGGGAGCAGAAGCCTCCTTCCGCAGGTGGGGAAATAC |
| mm4_intergenic_Gpc1|Ankmy1_chr1_92865929 |
CTGAAGTGGGGCCTAAGGACCTACCTGGGAGAGGGGAAGGGTCTCCAGAGGAGCTCCTCCTCTTTTCCAATCCCCTCGGG AGAGTCCCTGGTTGCCTTAGCCTCAATAAGTCAGTCAGTCTCTGCCCAC |
| mm4_intron_Pip4k2a_chr2_18908016 |
GTCAGAAAAGTCCTCATCTGCCCAGCCAGGGAGTCTCCAGAGAGGCTGCACCATACTTACTTGTTAAAAAGGTGGTTGTC AACCTTTATCTTTGAGTAGGCTTTGAAGTCATCAGGCATCAACATGACAGGGA |
| mm4_intron_Tmprss3_chr17_31183399 |
TGTTTGCCTAGCACCTGATGTGTGTGTGGCCAGGCCTTGCTTCCCCACAGCCCTTCCCCTCTCAGGAGAGACGGGGGTTG TAGACGTCTGACTTTCATTGCAGCAAGGCCTCT |
| mm4_intergenic_RP23-175E16.1|RP24-225I21.1/Kirrel3_chr9_34443998 |
GCTTCTCTGACAGGATGCCATGATGGTGAAGGTGGTTCTAACATCAGGGTGAACTCACCCATCCCCAAACTCTCGACAGT GGTTTCCCTAAGCAGTACTAGTAGACGA |
| mm4_intergenic_Bcas3|8430403D17Rik_chr11_85689447 |
AGGAAATGATGGGTGGCACCGTGACTCCTGCAGACCAAGAGACCGCAGTGGGGAGTCAGGGGATGAGAACTGAGGACCTG TAAGCCTAAGAGCCTACGGAGGCTTGTCAGACAGAG |
| mm4_intergenic_mmu-mir-7239|Cyfip2_chr11_46181852 |
AGAAGTGAGTGAGAACATCAGAGATAAAATTTAACCTTCCCTGGGCTGTCCAGAGTGGAAATTCTGAAGAGGAGACAGAC CATCACGATCTGGCTGTG |
| mm4_exon_Stx1b_chr7_127806722 | AAGATGTGCAGCATGGTCGCTGGGCCTCTCTGGATCCTCCGAGGATGTGAATTCTCCTCCCACTCCAGATGT |
| mm3_intron_Tmem182_chr1_40847903 |
TCTGCCAGCCTTTCAGACAGCTCTGCTTTGCGAGTCACTGTATCTCTCTGGAGATTCTGGGAATGTTTTCCAGACTTAAG TGATCGTGTTTGGTATTGACGGACA |
| mm3_intergenic_Gm22684|Anxa1_chr19_20094090 |
ACTCTCAATTCGTTAAGGCCATACACTAATGTCATAAGAGTGTATGTTAGAAAATGGTGGTTGACAGAAACACATCCCCG CACTCTCCTGTGAGGGATTCTGAAACAATTGCTCTGCTTCTCA |
| mm4_intron_Sh3bp4_chr1_89137221 |
GCTCTCTGTTCCTTGTGCCAAGCCTCCCACGGCCACTCCTCCAACAGGACCCGCCACTCTGGACAGACAGGGGCTGTTTT CTTCCTGAGTCCTGGAAGTGCCAATGTACTGCACGGTCTGAGG |
| mm4_intron_Kcnj3_chr2_55447478 |
GGGCATTTTGTGATCAGCCTGAGTTCAATAAGGCTGATCCTAACTTGTGGTGGTAAAATGCAGGAGAGAGTTATTGAAGA GGCTCTGGCATCTCCTCTGTGGAGCCTCAGGGGATGTGTCCTGTGTGCTTGTGTGT |
| mm3_intergenic_Rxrb|Col11a2_chr17_34039130 |
GATTACGAGTGGGCGTGGAACGGTCGCCCGTAGGCCCGCCCCTTCTGTCCCACTGTGCCCTGAGTCCCTCCCCGGACTCT TCATAGGGGCTCTGGGCCCCCAAGGGCAGCTGCCGTGGTGTGAAAGGACTA |
| mm4_intron_Brf1_chr12_112967602 |
AAACTGACAGTGGGACAGCCTCACCTCTGTGCCCATAACACAAGGTGCCCCTCTGCAGTGTCCATGGATGATATTGTGTG CCCAAGAAATACCGAGGAGGAACAAATCAGTAACAGGTCCTGGTCACTGC |
| mm4_exon_Gpr179_chr11_97347466 |
CAGAGATAGCGGCCAAGGACAAAACCCTGACTCTCCAGAGGGACACACTGTGGGGATGACAAATGACATTTGTTTGTATG ATGTTTTTCTCCGAAAGCTTTCCGTGCTGTGG |
| mm3_intergenic_Gm24371|BC024582_chr4_32540355 |
ACAGACTGTGTCAGGAACGTTCACTCAGAGATTCTCCAACCTTCCCCTGACACTCCAGAGAGATCATGGAAGTATGGGGA GAGAGGAGACAGATAAGGCATTAGCAGGAAGGAGCATACATGGGCAAGGACC |
| mm4_intergenic_Nt5m|Gm12714_chr11_59882093 |
CAGGAGGCTTTTCTTCGGGACCTCTCTGTAGAGGGGGGGGATGCCTGTAAGCCTCAGAGCCTGCCATGCATTTACAGGGC TGAAGCGAGTG |
| mm4_intron_Sdk2_chr11_113856508 |
AGGAACGACCCAAGCTTGTTCAAGTCTGTTGTGGACCATGCTGCACCCACAGGATGCTGCCTTTTCCTTTAAGCACATCA TCCCCCCATTCTACAGAGTGGAAGACTGAGAGAAGCTGAGCCAGACC |
| mm3_intron_Ddx41_chr13_55533905 |
AGAACTGTGTGAGGCCCATGCAGTAGCCAATCTAAAGGCTTCTTCTACCTCTCTGGACACTCAGGGGATGCCACCGAGAC CATCAGTACG |
| mm4_exon_Rit1_chr3_88730351 |
CAGGCCTTGTCCAGTGTTCTATCCCCTCTGAAGAGTCAGGCGAGGCCTGATGGCTTTTAGGCCTTTCATTCAACCAATGT CATTGTCCAAACAGAAGAGACTCAGGAGTCTGAGTAAAATTGTGTTCCCTTGCCCTGAG |
| mm4_intron_Efcab6_chr15_83921805 |
TGACAAGCTTTGCCATGACCTTGTCTTTCCAGTCCCTCACTGCAGAGTCCGGGGTTTCACTGTCCCTTTAAGACATCTCT GCCACCCCTCTGTAG |
| mm4_intron_Plekha7_chr7_116288632 |
GGGATGACTACCTGGGGACTCAGTCCACCCCTCTGCTGAGTCCTGGGAGGAGGGACGGGCAGGAGGTAGTCCTCAGTAGT GAGAGGAAGTTGCTGGC |
| mm4_intron_Csmd2_chr4_128553287 |
GTGGGAATGGGTGAGAGAGCTGAGAGAAAAGCCCCAAAACCCTGTCGGGCAGCACCATGGCCCTGCGGAGGTCATTTACA CAGCACCGGCCTCTCCCGAGTGGATAGTGAAGCAAGGCTCTTCCTCACCACGTTCTTCCG |
| mm4_intron_B4galt5_chr2_167330783 |
CACAGCACCTTGAGACTGGTAGGTTCTCAGATCCCAGACACACAGTGTGTGACTGCTCCAGTCTGCAGAGTCCAGGGATC TCCTCTCAGCTGTCAGGCCCGATTGAGGGTAGCAGGTGCAC |
| mm4_exon_Tnfaip2_chr12_111445097 |
CCGAGGCTGAATCAGAGACCTCCATGTCTGAGGCGTCCTCTGAGGACCTGATGCCATCCCCGGAGGCTCCCGATGGGGAG GAGGAGTCTGCGAAGAAGAAGGAGAAGAAGTCCAAAGGACTGGCCAACATGTTCA |
| mm4_intron_Bcar3_chr3_122420409 |
GTGACCAAAGTGTGTGTGGCGGGGGAGGGAGGGTGAGACTTGTGCCCTCGGGGGTCCTCGGATCCCGGGACTCTCGGGAG TGGAGCGTGGTGATGTGCCTCGAGCATCCCCATCTACTCTTGGGCTCCG |
| mm4_intron_Itga2_chr13_114838529 |
GGTTCACGGCAGTTCCAGTTTAGAGTCTGCTCTTTCTTTGGCTCCATTTCTGGCTTTCCATGCCCAGCCTCTGCAGAGAG GGTGTAGGCTTTTGCCAAGGTTCCCAATCCAGAGGCAGAAAAGCAGC |
| mm3_exon_Card11_chr5_140882027 |
CAGGAGCTGGCCAAAGAAGAAACTCGCTCTGGAGAGGCGGGGGCTGACTCGGGGGTCGCTCGTCGAAAGCATCTCTTCGG GC |
| mm4_intron_Ptrf_chr11_100965308 |
CTTGGGAGAGGGACAGTTGGGGAAACAGCAGACTCAGACAATAGCGATGTTCAGTAAGGCATGTAAAACTCCAAGGCAGC AACAGGTCCCAGCCTGCAGAGTCCGGGGAAGAGACAGGAACATCAGGGAGGTGGGTG |
| mm4_intron_Lrrc48_chr11_60382341 |
CCATTCGCACCTTCTGAGGACCAGGCTGGGGCCTGACTCACCCTCGAAACAAGTCCCTCTGGAGAGGCAGGTCATGGACC CCGCTAAACCTGACTCAGTGCCCTGCCTGTATCTCAGCCCACAAGAAGCTG |
| mm4_intron_Sept9_chr11_117201149 |
ATGTGCTCAGGTTTGGCACTGGGCCCTTCTCCCTCCCCAGCATGCTCCAGATGTTTGGAATGGTTGCGGGCATTGGGGCC ATTTCCCAGCCTCTCTGGCTGGCCCGGGGATGGACAGCCAAGGGCTGTGCTTTCTTCTCC |