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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314130_F | TCGTCGGCAGCGTCGTCGATGGACTAGCAGGCAG | 60.2 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314130_R | GTCTCGTGGGCTCGGAGCTACTAGGTTGCCCTTGG | 59.0 | 1.00 |
| mm2_exon_Mospd3_chr5_137599737_F | TCGTCGGCAGCGTCCTGGGGCTCGAAGAACAGAG | 60.1 | 0.69 |
| mm2_exon_Mospd3_chr5_137599737_R | GTCTCGTGGGCTCGGCAGCCACTATGACGTCCAGG | 60.1 | 0.69 |
| mm4_intergenic_Ppie|Hpcal4_chr4_123171971_F | TCGTCGGCAGCGTCTTTGTCACTGCCTGGCTCTT | 59.8 | 0.63 |
| mm4_intergenic_Ppie|Hpcal4_chr4_123171971_R | GTCTCGTGGGCTCGGCAAGGTCAGAGAGAGCCTGC | 60.1 | 0.63 |
| mm3_intergenic_Tfap2c|Gm22773_chr2_172739830_F | TCGTCGGCAGCGTCCCCAACCGTTCCTCTCTGAG | 59.7 | 0.63 |
| mm3_intergenic_Tfap2c|Gm22773_chr2_172739830_R | GTCTCGTGGGCTCGGGCCCTGCTTGGACTGAAGAT | 60.0 | 0.63 |
| mm4_exon_Olfr1418_chr19_11855674_F | Primer3: not found at this Tm | N.d. | 0.50 |
| mm4_exon_Olfr1418_chr19_11855674_R | Primer3: not found at this Tm | N.d. | 0.50 |
| mm4_exon_Olfr1417_chr19_11828747_F | TCGTCGGCAGCGTCGCACTGCCCAGGAAGATGAA | 60.3 | 0.50 |
| mm4_exon_Olfr1417_chr19_11828747_R | GTCTCGTGGGCTCGGTGGCTAGCCTGGAGATCTTCT | 60.0 | 0.50 |
| mm4_intron_Alkbh3_chr2_94001633_F | TCGTCGGCAGCGTCCGAGGAGCATGGGGAACAAA | 60.3 | 0.45 |
| mm4_intron_Alkbh3_chr2_94001633_R | GTCTCGTGGGCTCGGCCTTTACCCTGCTGTCCCTG | 60.0 | 0.45 |
| mm4_intergenic_Rps12-ps6|Astx1a_chrX_124396076_F | TCGTCGGCAGCGTCACCTGCAGAAGCCACAGTAG | 59.6 | 0.44 |
| mm4_intergenic_Rps12-ps6|Astx1a_chrX_124396076_R | GTCTCGTGGGCTCGGTAGACCGCAGATGTCCTGGA | 60.0 | 0.44 |
| mm4_intergenic_Rps12-ps18|Astx1b_chrX_124899691_F | Primer3: not found at this Tm | N.d. | 0.44 |
| mm4_intergenic_Rps12-ps18|Astx1b_chrX_124899691_R | Primer3: not found at this Tm | N.d. | 0.44 |
| mm4_intergenic_Rps12-ps17|Astx4c_chrX_125594116_F | TCGTCGGCAGCGTCACCTGCAGAAGCCACAGTAG | 59.6 | 0.44 |
| mm4_intergenic_Rps12-ps17|Astx4c_chrX_125594116_R | GTCTCGTGGGCTCGGTAGACCGCAGATGTCCTGGA | 60.0 | 0.44 |
| mm4_intergenic_Gm17361|Gm21616_chrX_125331078_F | TCGTCGGCAGCGTCACCTGCAGAAGCCACAGTAG | 59.6 | 0.44 |
| mm4_intergenic_Gm17361|Gm21616_chrX_125331078_R | GTCTCGTGGGCTCGGTAGACCGCAGATGTCCTGGA | 60.0 | 0.44 |
| mm4_intergenic_Kank1|Gm26207_chr19_25445856_F | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intergenic_Kank1|Gm26207_chr19_25445856_R | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intergenic_Atp2b4|Optc_chr1_133882276_F | TCGTCGGCAGCGTCACTGGGACCTTTTCAGCCAG | 59.8 | 0.41 |
| mm4_intergenic_Atp2b4|Optc_chr1_133882276_R | GTCTCGTGGGCTCGGCCTGCAAAGAGAAGAGGCCT | 59.6 | 0.41 |
| mm4_intron_Tmem163_chr1_127534211_F | TCGTCGGCAGCGTCTGCCTCTTAAATTGCCACCTCT | 59.9 | 0.41 |
| mm4_intron_Tmem163_chr1_127534211_R | GTCTCGTGGGCTCGGTGTCAAATGGTCTCTCTGATCAGA | 59.2 | 0.41 |
| mm4_intron_Chit1_chr1_134150169_F | TCGTCGGCAGCGTCGCGCTCTGTGGGAATCTAGA | 59.2 | 0.40 |
| mm4_intron_Chit1_chr1_134150169_R | GTCTCGTGGGCTCGGTCATGGTCCACTCGTTCCTG | 59.3 | 0.40 |
| mm4_intron_1700013N06Rik_chr12_106845809_F | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4_intron_1700013N06Rik_chr12_106845809_R | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4_intron_Rap1gap_chr4_137672454_F | TCGTCGGCAGCGTCGAAGGAAAACGGAGGCCAGA | 59.9 | 0.36 |
| mm4_intron_Rap1gap_chr4_137672454_R | GTCTCGTGGGCTCGGGCCTCCATTAACCACCTCCA | 59.3 | 0.36 |
| mm4_intron_Serpina12_chr12_104031403_F | TCGTCGGCAGCGTCGCTATCCTCCCACAATCCCC | 59.5 | 0.35 |
| mm4_intron_Serpina12_chr12_104031403_R | GTCTCGTGGGCTCGGTGTGGTCAATGTGGGAGGTG | 59.8 | 0.35 |
| mm4_intron_Dock5_chr14_67828329_F | TCGTCGGCAGCGTCTTGCTCTGCATGTTCCCTCC | 60.3 | 0.35 |
| mm4_intron_Dock5_chr14_67828329_R | GTCTCGTGGGCTCGGAGAATGAGCCACTCACGTCG | 60.1 | 0.35 |
| mm4_intron_Cap2_chr13_46566118_F | TCGTCGGCAGCGTCGCAAGAATTAACCAGGATCCCC | 59.0 | 0.35 |
| mm4_intron_Cap2_chr13_46566118_R | GTCTCGTGGGCTCGGGGTTGTGGGACTGGTGAGAC | 60.2 | 0.35 |
| mm4_exon_Smtn/Gm11946/Gm11947_chr11_3535152_F | TCGTCGGCAGCGTCAGAGTTCCTCACCCTCTCGG | 60.3 | 0.32 |
| mm4_exon_Smtn/Gm11946/Gm11947_chr11_3535152_R | GTCTCGTGGGCTCGGTTCGGAAACCATCCCCTTCC | 59.6 | 0.32 |
| mm4_intergenic_Gm11680|BC006965_chr11_112659839_F | TCGTCGGCAGCGTCCCCGCAGAACTAAAGCTCCA | 60.0 | 0.31 |
| mm4_intergenic_Gm11680|BC006965_chr11_112659839_R | GTCTCGTGGGCTCGGTGGCCTTCAGTCATCAACTCC | 59.9 | 0.31 |
| mm4_intergenic_Gm25440|Dtwd2_chr18_49617445_F | TCGTCGGCAGCGTCACCGGTGACTTGCTCTTTGA | 59.5 | 0.30 |
| mm4_intergenic_Gm25440|Dtwd2_chr18_49617445_R | GTCTCGTGGGCTCGGGGCAGTGCTGGACTAGAAGG | 60.1 | 0.30 |
| mm4_intron_Tyw1_chr5_130294200_F | TCGTCGGCAGCGTCGCAAAACACCATGCCAGTGA | 59.6 | 0.30 |
| mm4_intron_Tyw1_chr5_130294200_R | GTCTCGTGGGCTCGGATGAAGGCTTGCTGGTGACA | 59.8 | 0.30 |
| mm4_intron_3100003L05Rik_chr7_124673752_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intron_3100003L05Rik_chr7_124673752_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Gm10480|5730507C01Rik_chr12_18365632_F | TCGTCGGCAGCGTCTGCTGGCACTTGCTGGTTAT | 60.2 | 0.26 |
| mm4_intergenic_Gm10480|5730507C01Rik_chr12_18365632_R | GTCTCGTGGGCTCGGAGGGTGTACAGCTAAACGCC | 60.0 | 0.26 |
| mm4_intergenic_Gm14294|4930591A17Rik_chr2_179375681_F | TCGTCGGCAGCGTCTCAATCACACAGGTGCCCTC | 59.9 | 0.25 |
| mm4_intergenic_Gm14294|4930591A17Rik_chr2_179375681_R | GTCTCGTGGGCTCGGTGGGCTAAAACCTTGGTCGT | 59.5 | 0.25 |
| mm4_intergenic_Gm829|Gm12410_chr4_45711879_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Gm829|Gm12410_chr4_45711879_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_exon_Zfp276_chr8_123257172_F | TCGTCGGCAGCGTCTGGTGCTCATCTGGCATTCA | 59.6 | 0.25 |
| mm4_exon_Zfp276_chr8_123257172_R | GTCTCGTGGGCTCGGGCCTCTTTGTCTGCCTCCAT | 60.0 | 0.25 |
| mm4_intron_Tg_chr15_66720748_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Tg_chr15_66720748_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Shc3_chr13_51431556_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Shc3_chr13_51431556_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_exon_Cerk/Gm15569_chr15_86185934_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_exon_Cerk/Gm15569_chr15_86185934_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Tead1_chr7_112693140_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Tead1_chr7_112693140_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_9230114K14Rik|Gm25601_chr5_52327977_F | TCGTCGGCAGCGTCTGGCAGGAAGGGAAGAGAGA | 59.8 | 0.20 |
| mm4_intergenic_9230114K14Rik|Gm25601_chr5_52327977_R | GTCTCGTGGGCTCGGGCTTAGCCAAGGAAGCACCA | 60.6 | 0.20 |
| mm4_intergenic_Gm10108|Sema3a_chr5_13260574_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Gm10108|Sema3a_chr5_13260574_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_Nhsl2_chrX_102074122_F | TCGTCGGCAGCGTCTGCACTGTCCAGGTCATGAC | 59.9 | 0.18 |
| mm4_intron_Nhsl2_chrX_102074122_R | GTCTCGTGGGCTCGGATTGGCAGTGCAGATAGGGG | 59.8 | 0.18 |
| mm4_intergenic_Mpl|Tie1_chr4_118457524_F | TCGTCGGCAGCGTCATCTTCTCCGGCACTGTGTG | 60.0 | 0.18 |
| mm4_intergenic_Mpl|Tie1_chr4_118457524_R | GTCTCGTGGGCTCGGGACGTGGGGCTGTATCTGAC | 60.1 | 0.18 |
| mm4_intergenic_Gm6274|Slc25a43_chrX_36674994_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm6274|Slc25a43_chrX_36674994_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm9578|Gm22891_chr14_80447011_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm9578|Gm22891_chr14_80447011_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm25672|Barhl2_chr5_106442368_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm25672|Barhl2_chr5_106442368_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_1700017N19Rik|Gm26278_chr10_100632526_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_1700017N19Rik|Gm26278_chr10_100632526_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Gldn_chr9_54336512_F | TCGTCGGCAGCGTCAGGTACATGAGAGCACTCTGC | 59.5 | 0.13 |
| mm4_intron_Gldn_chr9_54336512_R | GTCTCGTGGGCTCGGTTGGCTAACTCCAGCTTGCA | 59.8 | 0.13 |
| mm4_intron_Pparg_chr6_115361422_F | TCGTCGGCAGCGTCGTGACAGACAAGGTGAGGGG | 59.9 | 0.12 |
| mm4_intron_Pparg_chr6_115361422_R | GTCTCGTGGGCTCGGTACGCAGCCCTGTCAGAATG | 60.1 | 0.12 |
| mm4_intergenic_Rasgef1c|Rnf130_chr11_50005686_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Rasgef1c|Rnf130_chr11_50005686_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Mir694|Ccbe1_chr18_66223900_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Mir694|Ccbe1_chr18_66223900_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_exon_Txnrd2_chr16_18472837_F | TCGTCGGCAGCGTCCTGGAGTATGGCTGTGTGGG | 60.1 | 0.11 |
| mm4_exon_Txnrd2_chr16_18472837_R | GTCTCGTGGGCTCGGGCCCCTGTTTACCAGACCTC | 60.0 | 0.11 |
| mm4_intergenic_Neurod2|Ppp1r1b_chr11_98340810_F | TCGTCGGCAGCGTCTTCTAGGGAAGCAGGGACCA | 59.8 | 0.11 |
| mm4_intergenic_Neurod2|Ppp1r1b_chr11_98340810_R | GTCTCGTGGGCTCGGGGTGGGCTTTCTGTCTCTGG | 60.3 | 0.11 |
| mm3_intergenic_Amer2|Spata13_chr14_60597417_F | TCGTCGGCAGCGTCCTGAGTGGGAGGCCAAGAAG | 60.0 | 0.11 |
| mm3_intergenic_Amer2|Spata13_chr14_60597417_R | GTCTCGTGGGCTCGGAAGAAGGTCTGTGGGTGCAG | 59.8 | 0.11 |
| mm4_exon_Tmem132e_chr11_82389258_F | TCGTCGGCAGCGTCCACCTCATCCCAGTGTTCCC | 60.0 | 0.11 |
| mm4_exon_Tmem132e_chr11_82389258_R | GTCTCGTGGGCTCGGGAGACCGAGGAATCTACCCC | 58.6 | 0.11 |
| mm4_intergenic_Gm10087|Sbf2_chr7_110557756_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm10087|Sbf2_chr7_110557756_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm22894|Ky_chr9_102408351_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm22894|Ky_chr9_102408351_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Ppm1h_chr10_122829055_F | TCGTCGGCAGCGTCCCTTGGTTTTCTGCTCTTGCC | 60.0 | 0.10 |
| mm4_intron_Ppm1h_chr10_122829055_R | GTCTCGTGGGCTCGGACACAGTTCTACGCCATGCA | 59.9 | 0.10 |
| mm4_intron_Pamr1_chr2_102633652_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Pamr1_chr2_102633652_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Pcdh20_chr14_88468015_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Pcdh20_chr14_88468015_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Lepre1_chr4_119233069_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Lepre1_chr4_119233069_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Plk2|Actbl2_chr13_111172012_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Plk2|Actbl2_chr13_111172012_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm16876|Ston2_chr12_91695948_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm16876|Ston2_chr12_91695948_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intergenic_Wdr61|Crabp1_chr9_54740727_F | TCGTCGGCAGCGTCGCTGCAGGACTGAAGGGAAT | 60.0 | 0.07 |
| mm3_intergenic_Wdr61|Crabp1_chr9_54740727_R | GTCTCGTGGGCTCGGACAAGAGACTGGCTGCTGTG | 60.2 | 0.07 |
| mm4_intergenic_Cadm1|Nxpe2_chr9_47894967_F | TCGTCGGCAGCGTCGCGGTCTCTCTGAAGCTGTT | 60.0 | 0.06 |
| mm4_intergenic_Cadm1|Nxpe2_chr9_47894967_R | GTCTCGTGGGCTCGGCTGCACTCAGCCTTCTCCTC | 60.1 | 0.06 |
| mm4_intron_Slc12a4_chr8_105950289_F | TCGTCGGCAGCGTCACCGAAGAGGATCACACTGC | 59.7 | 0.06 |
| mm4_intron_Slc12a4_chr8_105950289_R | GTCTCGTGGGCTCGGAGGACAAGGACAAGGCGATG | 60.0 | 0.06 |
| mm4_exon_Pcdhga9_chr18_37822040_F | TCGTCGGCAGCGTCACAGGACCTGCTTTTCTCCG | 59.9 | 0.06 |
| mm4_exon_Pcdhga9_chr18_37822040_R | GTCTCGTGGGCTCGGCAGCGTACTAGAGCGGGAAC | 60.2 | 0.06 |
| mm4_exon_Meis3_chr7_16178028_F | TCGTCGGCAGCGTCGAGCTGGACAACCTGGTGAG | 60.3 | 0.06 |
| mm4_exon_Meis3_chr7_16178028_R | GTCTCGTGGGCTCGGGGGCTAGTGGTTTCCTGTCC | 60.0 | 0.06 |
| mm4_intron_Nfasc_chr1_132590542_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Nfasc_chr1_132590542_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Sall4_chr2_168756003_F | TCGTCGGCAGCGTCACGTTAACCCCGAGGACAAC | 59.9 | 0.05 |
| mm4_exon_Sall4_chr2_168756003_R | GTCTCGTGGGCTCGGGTCGCTCGATGCCTTGAAAC | 59.9 | 0.05 |
| mm4_intergenic_Gm9436|Gm5755_chrX_17725957_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm9436|Gm5755_chrX_17725957_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Emx2os/Emx2_chr19_59459123_F | TCGTCGGCAGCGTCACCCCAAACAAACGAGTCCC | 60.4 | 0.05 |
| mm4_exon_Emx2os/Emx2_chr19_59459123_R | GTCTCGTGGGCTCGGGAAACATGCTGCGCCGAG | 59.9 | 0.05 |
| mm3_intergenic_Gm22016|Cdc73_chr1_143309527_F | TCGTCGGCAGCGTCGGCCTCACCCACATATTAGTCT | 59.2 | 0.05 |
| mm3_intergenic_Gm22016|Cdc73_chr1_143309527_R | GTCTCGTGGGCTCGGGGCTGACAGCTCACATTTTCT | 58.8 | 0.05 |
| mm4_intron_Myl3_chr9_110764615_F | TCGTCGGCAGCGTCCAGAGGCCACTGCTACTCTG | 59.8 | 0.05 |
| mm4_intron_Myl3_chr9_110764615_R | GTCTCGTGGGCTCGGCAGTGGATCCATGTGGGTGG | 60.3 | 0.05 |
| mm4_intergenic_Gm16876|Ston2_chr12_91698384_F | TCGTCGGCAGCGTCACATTTGGGTCCATGGGTCC | 59.9 | 0.05 |
| mm4_intergenic_Gm16876|Ston2_chr12_91698384_R | GTCTCGTGGGCTCGGCACCTCCCAGATGCTGTCTC | 59.8 | 0.05 |
| mm4_intron_Phf15_chr11_51824825_F | TCGTCGGCAGCGTCAGGCTCTCCCACCTCTTTCT | 59.8 | 0.05 |
| mm4_intron_Phf15_chr11_51824825_R | GTCTCGTGGGCTCGGTCTTACTCCATGTGCCCTGC | 59.7 | 0.05 |
| mm4_intergenic_Efcab5|Ssh2_chr11_77216063_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Efcab5|Ssh2_chr11_77216063_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intron_Msi2_chr11_88483786_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intron_Msi2_chr11_88483786_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Sh3tc1|Ablim2_chr5_35734631_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Sh3tc1|Ablim2_chr5_35734631_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Fhdc1_chr3_84480142_F | TCGTCGGCAGCGTCGCATAAGTGCCACGAAAGCT | 59.1 | 0.04 |
| mm4_intron_Fhdc1_chr3_84480142_R | GTCTCGTGGGCTCGGGGACCCTGAGCTCGGTGTAG | 61.6 | 0.04 |
| mm3_exon_Ppm1g_chr5_31203121_F | TCGTCGGCAGCGTCGTCCGGTTGACCACTAGTCC | 59.7 | 0.04 |
| mm3_exon_Ppm1g_chr5_31203121_R | GTCTCGTGGGCTCGGTTCAAGCCCCGAAACACAGT | 60.1 | 0.04 |
| mm4_intron_Camk2g_chr14_20793697_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Camk2g_chr14_20793697_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Alx4|Ext2_chr2_93643611_F | TCGTCGGCAGCGTCACCAGGCACCTCAGAAAACC | 60.1 | 0.03 |
| mm4_intergenic_Alx4|Ext2_chr2_93643611_R | GTCTCGTGGGCTCGGGCTTTCCCACCCGTTGAGTA | 59.9 | 0.03 |
| mm4_intergenic_Ifltd1|Gm25373_chr6_145460430_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Ifltd1|Gm25373_chr6_145460430_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Atp8a2_chr14_60005797_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Atp8a2_chr14_60005797_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Ncoa1|Gm3625_chr12_4477294_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Ncoa1|Gm3625_chr12_4477294_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Efnb2|4921522P10Rik_chr8_8661215_F | TCGTCGGCAGCGTCCTTTGTGTGCGGGGAGGG | 60.6 | 0.03 |
| mm4_intergenic_Efnb2|4921522P10Rik_chr8_8661215_R | GTCTCGTGGGCTCGGCCCCGCGTTTATAGTGCTCT | 59.8 | 0.03 |
| mm4_intron_Aldh1l1_chr6_90585829_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Aldh1l1_chr6_90585829_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Vsig8_chr1_172562113_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Vsig8_chr1_172562113_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Fastkd3|1700001L19Rik_chr13_68594431_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Fastkd3|1700001L19Rik_chr13_68594431_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Igfbp3_chr11_7213723_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Igfbp3_chr11_7213723_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Ppcdc_chr9_57417147_F | TCGTCGGCAGCGTCTCCCGGGGATACAGCAACTA | 60.0 | 0.02 |
| mm4_intron_Ppcdc_chr9_57417147_R | GTCTCGTGGGCTCGGATGTTGATGCCTGGGACGAG | 60.1 | 0.02 |
| mm4_intergenic_Ppp1r37|Gemin7_chr7_19563866_F | TCGTCGGCAGCGTCTAACCACCAACCTGCAGCAT | 59.8 | 0.02 |
| mm4_intergenic_Ppp1r37|Gemin7_chr7_19563866_R | GTCTCGTGGGCTCGGCCCTCTTGGTGATGTGGTCC | 60.0 | 0.02 |
| mm4_exon_Pmvk_chr3_89468718_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Pmvk_chr3_89468718_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_1700116B05Rik|Gm24925_chr16_50848041_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_1700116B05Rik|Gm24925_chr16_50848041_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Kdelr2|Daglb_chr5_143458212_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Kdelr2|Daglb_chr5_143458212_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_G930045G22Rik|Mir148a_chr6_50912362_F | TCGTCGGCAGCGTCTCCAAGGCTCACGAGTTCAC | 59.9 | 0.02 |
| mm4_intergenic_G930045G22Rik|Mir148a_chr6_50912362_R | GTCTCGTGGGCTCGGTGTGATCAAACTGCTGCCCT | 59.8 | 0.02 |
| mm4_intron_Epb4.1_chr4_131976623_F | TCGTCGGCAGCGTCCCGGCCTTACACTTGCTAGT | 59.7 | 0.01 |
| mm4_intron_Epb4.1_chr4_131976623_R | GTCTCGTGGGCTCGGTCTAGTAGGCCTGGCAGGAG | 60.1 | 0.01 |
| mm4_intergenic_Adat2|Aig1_chr10_13566726_F | TCGTCGGCAGCGTCGGCCACCTTTCTTGAGAGCT | 59.9 | 0.01 |
| mm4_intergenic_Adat2|Aig1_chr10_13566726_R | GTCTCGTGGGCTCGGGGTGATAGGGGCAAGACAGG | 59.8 | 0.01 |
| mm4_intron_Zfhx3_chr8_108900931_F | TCGTCGGCAGCGTCGCTTATTAGCGGAAGGGGAA | 57.3 | 0.01 |
| mm4_intron_Zfhx3_chr8_108900931_R | GTCTCGTGGGCTCGGCTAGGTCCGGGGTAAGTCCA | 60.0 | 0.01 |
| mm4_intergenic_A930001C03Rik|Syt12/A930001C03Rik_chr19_4444047_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_A930001C03Rik|Syt12/A930001C03Rik_chr19_4444047_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm16045|Sept9_chr11_117266133_F | TCGTCGGCAGCGTCCATCCTCTACATCCGCCCAC | 59.9 | 0.01 |
| mm4_intergenic_Gm16045|Sept9_chr11_117266133_R | GTCTCGTGGGCTCGGCTGCAGAGTCCCAGGCAC | 60.0 | 0.01 |
| mm4_intergenic_Slc6a13|Gm24855_chr6_121325173_F | TCGTCGGCAGCGTCACTTCTGCATCTGGAAGGGG | 59.3 | 0.01 |
| mm4_intergenic_Slc6a13|Gm24855_chr6_121325173_R | GTCTCGTGGGCTCGGCTGCCTGGACCAGCCTAAAG | 60.3 | 0.01 |
| mm4_exon_Efcab7_chr4_99901764_F | TCGTCGGCAGCGTCAGTGTGATGAGGATGCCTGG | 59.4 | 0.01 |
| mm4_exon_Efcab7_chr4_99901764_R | GTCTCGTGGGCTCGGGGCTTGTTTCTGAGAATGAGGG | 59.2 | 0.01 |
| mm4_exon_Gpam_chr19_55099362_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Gpam_chr19_55099362_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Agpat9|Gm20484_chr5_101353564_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Agpat9|Gm20484_chr5_101353564_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Cetn4_chr3_37312397_F | TCGTCGGCAGCGTCTCACCATATCCCCTCCCTGC | 60.7 | 0.01 |
| mm4_exon_Cetn4_chr3_37312397_R | GTCTCGTGGGCTCGGTCAATCACGGAAGCTGGTGG | 60.3 | 0.01 |
| mm4_intron_Casz1_chr4_148804926_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Casz1_chr4_148804926_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Wdfy3_chr5_101934818_F | TCGTCGGCAGCGTCCTCCTCACAAACCCCAGCTG | 60.6 | 0.00 |
| mm4_intron_Wdfy3_chr5_101934818_R | GTCTCGTGGGCTCGGATTGAGTGCTCGCCTCTGTC | 60.1 | 0.00 |
| mm4_intron_Tjp2_chr19_24206746_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Tjp2_chr19_24206746_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Pex7_chr10_19874590_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Pex7_chr10_19874590_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Gm4779_chrX_101791737_F | TCGTCGGCAGCGTCTGGAAGGGACTAATGGGGTC | 58.4 | 0.00 |
| mm4_intron_Gm4779_chrX_101791737_R | GTCTCGTGGGCTCGGACCTTTATGCTGCCTCTGGG | 59.7 | 0.00 |
| mm4_intron_Fam53b_chr7_132811110_F | TCGTCGGCAGCGTCTAAAGAGCTCTGCAGGCACC | 60.0 | 0.00 |
| mm4_intron_Fam53b_chr7_132811110_R | GTCTCGTGGGCTCGGTGATGGGAGCTCAGCCAAAG | 60.0 | 0.00 |
| mm4_intron_Dym_chr18_75119541_F | TCGTCGGCAGCGTCTGAGCAGGTTCTCCAAGCAG | 59.9 | 0.00 |
| mm4_intron_Dym_chr18_75119541_R | GTCTCGTGGGCTCGGGCTGTGTGGCAATCATGACC | 59.8 | 0.00 |
| mm4_intron_Adcy5_chr16_35267311_F | TCGTCGGCAGCGTCACCCTCAATGAGCTCTTCGC | 60.1 | 0.00 |
| mm4_intron_Adcy5_chr16_35267311_R | GTCTCGTGGGCTCGGCAACACCGGTCTCCCCTAAG | 59.7 | 0.00 |
| mm4_intron_9530036O11Rik_chr5_28732628_F | TCGTCGGCAGCGTCATGTGGTCTGCTCTGTGGTG | 59.9 | 0.00 |
| mm4_intron_9530036O11Rik_chr5_28732628_R | GTCTCGTGGGCTCGGGACGATGTGGGAAAGGGAGG | 60.1 | 0.00 |
| mm4_intergenic_Gm5449|Drd1a_chr13_53565550_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm5449|Drd1a_chr13_53565550_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm13372/Col5a1|Col5a1_chr2_28026746_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm13372/Col5a1|Col5a1_chr2_28026746_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm13056|Epha2_chr4_141297476_F | TCGTCGGCAGCGTCTCAGGAGGACTCAGAGGCTT | 59.5 | 0.00 |
| mm4_intergenic_Gm13056|Epha2_chr4_141297476_R | GTCTCGTGGGCTCGGCCTGCCTCTGAATCCCACAG | 60.1 | 0.00 |
| mm4_intergenic_Dlc1|AI429214_chr8_36964067_F | TCGTCGGCAGCGTCTCTGTGTGCCTTGAGTGGAC | 59.8 | 0.00 |
| mm4_intergenic_Dlc1|AI429214_chr8_36964067_R | GTCTCGTGGGCTCGGAACCCATGAAGGACAGGAGC | 59.6 | 0.00 |
| mm4_intergenic_4930588K23Rik|Gp1bb_chr16_18613584_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_4930588K23Rik|Gp1bb_chr16_18613584_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Cenpb_chr2_131179381_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Cenpb_chr2_131179381_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314130 |
GTCGATGGACTAGCAGGCAGTGCTGCAGGGCTGGGCGCCGAGCCACGGTCTGCTGGAGCGGCCATGCTTGGCCCGGGACC CCCAGTCCCCTCCGCGGACAGCCTCTCTGGCCAAGGGCAACCTAGTAGCT |
| mm2_exon_Mospd3_chr5_137599737 |
CTGGGGCTCGAAGAACAGAGGTGATGTCTTTTCTTCCAACCACTCGTCCCTCAGTCCCCTCCTCAGACAGCTCAATGCGG AATCTGTCCTGGACGTCATAGTGGCTG |
| mm4_intergenic_Ppie|Hpcal4_chr4_123171971 |
TTTGTCACTGCCTGGCTCTTCCACCCACCTTTAAAAACTCTTTAGAGCCACTCAAAACCCCTTCAAAGTGACCCCACAGT CCCCTCTGCTGGCAACTGTTTTTCACTGCAGGCTCTCTCTGACCTTG |
| mm3_intergenic_Tfap2c|Gm22773_chr2_172739830 |
CCCAACCGTTCCTCTCTGAGGAAGTTGTTAGCGGAGGGGACTGCGGTCACAATACTGTTTGTGACTGTTTATCTTCAGTC CAAGCAGGGC |
| mm4_exon_Olfr1417_chr19_11828747 |
GCACTGCCCAGGAAGATGAAGAAGAACATCTGGGCACCACAGCCTGCCAAGGAAACCACAGTCCTCTCTGTGGACAGGAT GCTGGCCAGAGTCAGGGGGGCTATGGTAGAAGAATAGAAGATCTCCAGGCTAGCCA |
| mm4_intron_Alkbh3_chr2_94001633 |
CGAGGAGCATGGGGAACAAAAGGAGAGGAGGGATGACCCCAGTCCCTTCCTCAGACAGGAAATGGGAGGTGGCTAGGAGA ACCTGGAAGGGCTACAGAGGAAATAAGGCATGCTGGCAGGGACAGCAGGGTAAAGG |
| mm4_intergenic_Rps12-ps6|Astx1a_chrX_124396076 |
ACCTGCAGAAGCCACAGTAGGAAAGTCCCTGTCTGCTGAGGAGACTGTGGGAACTTTGCTTCCCACTCCAGGACATCTGC GGTCTA |
| mm4_intergenic_Rps12-ps17|Astx4c_chrX_125594116 |
ACCTGCAGAAGCCACAGTAGGAAAGTCCCTGTCTGCTGAGGAGACTGTGGGAACTTTGCTTCCCACTCCAGGACATCTGC GGTCTA |
| mm4_intergenic_Gm17361|Gm21616_chrX_125331078 |
ACCTGCAGAAGCCACAGTAGGAAAGTCCCTGTCTGCTGAGGAGACTGTGGGAACTTTGCTTCCCACTCCAGGACATCTGC GGTCTA |
| mm4_intergenic_Atp2b4|Optc_chr1_133882276 |
ACTGGGACCTTTTCAGCCAGGCAGGCAAGGTCCCTAGGGTCCTGGTGTCCCACAGGACGCTCAAAACTCAAATACATCTT GCTTCTGTCTGGTGTCAGAAGAGGGGACTGGGGACCTCCTAGGCCTCTTCTCTTTGCAGG |
| mm4_intron_Tmem163_chr1_127534211 |
TGCCTCTTAAATTGCCACCTCTTTAAGTAAAATACCTGATGCTGGCCACAGTCCCCTCAGAGGATAGTTTAGTAAAACAA TTCTGATCAGAGAGACCATTTGACA |
| mm4_intron_Chit1_chr1_134150169 |
GCGCTCTGTGGGAATCTAGAAAAGAGTAGAAGCTGTTTAGGGAGGAAGGATTGTACAAGCCCTAAAAGTCAGAAGATAGG CAGGTGTCCAGGGAGGAGACTGTGGGCAGTCGGGAGGGAGCAGGAACGAGTGGACCATGA |
| mm4_intron_Rap1gap_chr4_137672454 |
GAAGGAAAACGGAGGCCAGATAGTGGGGTTCAGCCCTCTGTCCCTCTCCTGATATCTGACTTGAATCTCTGAGAGATAGA TGCCCTCGTCCAGAGTCCCCTCCTCGGGCAGATGGAGGTGGTTAATGGAGGC |
| mm4_intron_Serpina12_chr12_104031403 |
GCTATCCTCCCACAATCCCCCACCACAGTTCCCTCCCAGAACAGCTTGACCCCCTCCCTTTACTATACTCAACACCAGAC AATTCCACCACCTCCCACATTGACCACA |
| mm4_intron_Dock5_chr14_67828329 |
TTGCTCTGCATGTTCCCTCCCCGGTCCCCACCCAAAGTCCCCTCACCGGGCAGCCTGTGTACCTTGTCCTTTGTGATTCA CTTCCTTTGCAGCTATGACTTTATTTAGGACCGACGTGAGTGGCTCATTCT |
| mm4_intron_Cap2_chr13_46566118 |
GCAAGAATTAACCAGGATCCCCTGAGAACTGCCTTAATCCCCTCTGAGGACAGCCTCCCAGTTGACCCAATTACTTCCCT CTACTCACGTCTCACCAGTCCCACAACC |
| mm4_exon_Smtn/Gm11946/Gm11947_chr11_3535152 |
AGAGTTCCTCACCCTCTCGGTCACCCCCACTGTGTGTGGAGGGGACTGCGGGAGGGAGGGAAGGAAATACAAAGAACTAT TGTCCGCCAGGGGGAAGGGGATGGTTTCCGAA |
| mm4_intergenic_Gm11680|BC006965_chr11_112659839 |
CCCGCAGAACTAAAGCTCCACCCCCACCGCATAAAGTCTTCTGAACCGCTGAAAGGGAAAACATCTCCCCAGTCCCCTCA GCTGACTGGAAACACATCCACCAGCTTAACTCTACAGAGGAGTTGATGACTGAAGGCCA |
| mm4_intergenic_Gm25440|Dtwd2_chr18_49617445 |
ACCGGTGACTTGCTCTTTGATCAGTTATATTGCGTCCAAGATTCCAGTTTCCTTAGTCCCCTGTGCAGACAGCCTGAATC AGATTACTTATACCCTACAGGAGTTAAAGTCAACAACTCCTTCTAGTCCAGCACTGCC |
| mm4_intron_Tyw1_chr5_130294200 |
GCAAAACACCATGCCAGTGATTCCTTCCAGATGGCCTCAGCTGGAGCCCACTTCACACCAGCTGCGTCCCCAGTCCCCTC CATGGTCACCGTCTTACTCTGTGTCACCAGCAAGCCTTCAT |
| mm4_intergenic_Gm10480|5730507C01Rik_chr12_18365632 |
TGCTGGCACTTGCTGGTTATAGCAGCAAGCCAAGCACTTCATTCTCAAGAGAATGCATTTTGTTTTGTTTTGAACCTCTG TTCCCTTTGCGGACAGCTCTGATGATGGCGTTTAGCTGTACACCCT |
| mm4_intergenic_Gm14294|4930591A17Rik_chr2_179375681 |
TCAATCACACAGGTGCCCTCTCACCTCTGCCCGCAGAGGGGACCGAGGTCCCCAACATTCCTCAGGAGAGGTTCCTAGAA CCTTGTCCATAAAACCCACAATACGACCAAGGTTTTAGCCCA |
| mm4_exon_Zfp276_chr8_123257172 |
TGGTGCTCATCTGGCATTCAGTCTTCCTCTAAGTCTGTCCTTAGACCACAGGGGACTGGTTGTTCCTGTAGGCTGAGGGG ACTGAGGGAGAGAGAAGAATGGGGTTATTGTCCGCAGGACATGGAGGCAGACAAAGAGGC |
| mm4_intergenic_9230114K14Rik|Gm25601_chr5_52327977 |
TGGCAGGAAGGGAAGAGAGAGGGCTGTTTGCAGAGGGGACAGGGGGCACAGTGGGGAGGCAGAAGGGAAAGATTGCAGGT AGCTGCTCTATTCCAGACTTGTGCTGACAGAGCCCCACCATGGTGCTTCCTTGGCTAAGC |
| mm4_intron_Nhsl2_chrX_102074122 |
TGCACTGTCCAGGTCATGACTCCCCAGGGAGCAGAGGCTTGCCGAAGAGGGGACTGTGGCTGCTCTTCCCTCACCGGCCC CTATCTGCACTGCCAAT |
| mm4_intergenic_Mpl|Tie1_chr4_118457524 |
ATCTTCTCCGGCACTGTGTGCCTGCCTTAGCCCATCCTCCCTTCAGGAAGCTGGGCCCCAATCCCCCCCGGGGCCAGCCC CTCAGGTTCCTGTCAGATACAGCCCCACGTC |
| mm4_intron_Gldn_chr9_54336512 |
AGGTACATGAGAGCACTCTGCTCTGCAGGATGTCCCCAGTGGGGACTGCGGAGCAGTGAGGTGAGGTGCAAGCTGGAGTT AGCCAA |
| mm4_intron_Pparg_chr6_115361422 |
GTGACAGACAAGGTGAGGGGGGGTCTCGGCTTGTGGGGTCTGGATCTGACTGGCTAGGTGACTGGGTCTCCGCTGAGGGG ACTCGGGATGAGAAGTCACATTCTGACAGGGCTGCGTA |
| mm4_exon_Txnrd2_chr16_18472837 |
CTGGAGTATGGCTGTGTGGGGCTGTCTGAGGAGGAGGCTGTGGCTCTCCATGGCCAGGAGCATGTAGAGGTAGGGAACTC TCCTCCACAGAACCCCTCTATAGATTGGAGGTCTGGTAAACAGGGGC |
| mm4_intergenic_Neurod2|Ppp1r1b_chr11_98340810 |
TTCTAGGGAAGCAGGGACCACAGGAGCTCACATGGGGGAGGAGCTTCCCCTCTTTTTCCTGCTGCAGGCTGGGAAGTGGC CCGCGGCTGCCAGAGGAGGGGCCTGGGGAAGGAGACCAGAGACAGAAAGCCCACC |
| mm3_intergenic_Amer2|Spata13_chr14_60597417 |
CTGAGTGGGAGGCCAAGAAGGGGACTTCACCCAAACTGCCTGCAGTCCCCTCAAGGGACAGCAGCCTGCAGTCAGCTGGG GAGCTGCACCCACAGACCTTCTT |
| mm4_exon_Tmem132e_chr11_82389258 |
CACCTCATCCCAGTGTTCCCCTGGACTCTCCGCCGTCGCTACCAGCACGGTTCCCGGCTGCAGCGGGAGTCCGCTGAGGG GACCGAGGTCGGTCCAGGGGAGATTGGGGCGGGGGGGGGGTAGATTCCTCGGTCTC |
| mm4_intron_Ppm1h_chr10_122829055 |
CCTTGGTTTTCTGCTCTTGCCTTCTTTGAAGATTTTAGCTACATGTCCTGTCTTTCTACATCCCTCCTCTGTCCCCTCTG CTGTCAGCTACTGCCACATAACTTACTGCATGGCGTAGAACTGTGT |
| mm3_intergenic_Wdr61|Crabp1_chr9_54740727 |
GCTGCAGGACTGAAGGGAATCCTGGGAGATCTTGCTATTCAGGGACACACACCCCCCAGCCCCCTCCCCGGCCAGCCCAG GAGGCTGTGAGGCACAGCAGCCAGTCTCTTGT |
| mm4_intergenic_Cadm1|Nxpe2_chr9_47894967 |
GCGGTCTCTCTGAAGCTGTTTTCTTCCAACCAGGGCCGTGTGCTATGTGTGCTCTGTATGGTCTGGGAAAACGTGACTTT TGCAGAGCTGTCCCGGGAGGGAGCTGGGGAGAGGAGAAGGCTGAGTGCAG |
| mm4_intron_Slc12a4_chr8_105950289 |
ACCGAAGAGGATCACACTGCTGAAGTCTGGACTGTGTTAAGGAAACTAGAAGCTCGCCAGACCCCAGTCCACTCCCCACA CAGCACCCCAGTGATCTTCACAGCCATCGCCTTGTCCTTGTCCT |
| mm4_exon_Pcdhga9_chr18_37822040 |
ACAGGACCTGCTTTTCTCCGGCCTCGGATGGCAGTGACTTCACTTTCCTCAGGCCCCTCAGCGTTCAGCAGCCCTCAGCC TTGGCGCTGGAACCCGAGGCCTTGCGTTCCCGCTCTAGTACGCTG |
| mm4_exon_Meis3_chr7_16178028 |
GAGCTGGACAACCTGGTGAGGCCCAGGCCCTCCCCGTGTCCCCTCCGCAGCCAGCTCTTGTCCTTCCACTTCACCTGTAT TCCTGGGGTTGGCTGAGGGGTGAGGACAGGAAACCACTAGCCC |
| mm4_exon_Sall4_chr2_168756003 |
ACGTTAACCCCGAGGACAACGGGCTGGCTGCGGAGGGGACGCTGGCATGAGGTAGCTTGGCTTGTTTCAAGGCATCGAGC GAC |
| mm4_exon_Emx2os/Emx2_chr19_59459123 |
ACCCCAAACAAACGAGTCCCCCATTCTCCTCGGTCCTCGCCGCGGGCAGCGGGCGGCGGAGGCAGCGCGCCGCGGTCGCC GGGAGCAGGGAGCCCAGCGCGCCCGCTCCTCGGCGCAGCATGTTTC |
| mm3_intergenic_Gm22016|Cdc73_chr1_143309527 |
GGCCTCACCCACATATTAGTCTGTTTTTTGAACAATGAGAAGTTGTTTTCTTAATGGCATTTGCTGTTTATCGCTGACTG CGGAGGGGTCTGCGGAGGAGCATTCCTAAGATTTTTTCTAGAAAATGTGAGCTGTCAGCC |
| mm4_intron_Myl3_chr9_110764615 | CAGAGGCCACTGCTACTCTGGCTGTCTCCTGAGGGGTCTGTGGAGTCCTCTCCACCCACATGGATCCACTG |
| mm4_intergenic_Gm16876|Ston2_chr12_91698384 |
ACATTTGGGTCCATGGGTCCTGGAAGAGCGTGATGCTGTCCGCAGAGAGGGCTCTGGAAGCTGGCGTACAGGAGACAGCA TCTGGGAGGTG |
| mm4_intron_Phf15_chr11_51824825 |
AGGCTCTCCCACCTCTTTCTAGATGTTCAGATGGGTGGCACCTGTCCTTGGAGGGGTCTGTGGTAGCCCCCTGAGTCTTT GGAGCCTACCAAAGGCTCAAACTGGGCAGGGCACATGGAGTAAGA |
| mm4_intron_Fhdc1_chr3_84480142 | GCATAAGTGCCACGAAAGCTAATCAAACCTCCGAGGTCCCACCCGCGGACAGCGCTACACCGAGCTCAGGGTCC |
| mm3_exon_Ppm1g_chr5_31203121 |
GTCCGGTTGACCACTAGTCCCTCTTGGCCTTCTTTTTGTCACTGTTGCCGGTGTCTTCAGCCCCCTCCGTGGACAGTGCC TCCTCCAGTTTCCTCTTGCCACTCTCTGCCTGAAGCTCTACTGTGTTTCGGGGCTTGAA |
| mm4_intergenic_Alx4|Ext2_chr2_93643611 |
ACCAGGCACCTCAGAAAACCCGAGTCCTGGCCAGGAGCGCAGACCGCGTAGGGGACCGCGGCCAGCCTGTCTGCGGTGTC TGGAAGTAGCCAACATCAGGAAAGCACAGTTTAGTACTCAACGGGTGGGAAAGC |
| mm4_intergenic_Efnb2|4921522P10Rik_chr8_8661215 |
CTTTGTGTGCGGGGAGGGCGCCAGGACCCGCTCTGCGCGCAGCTCCGCGATCCGACCGGTGCCTCTGTCCCCGCCGCGGA GAGTCAGCGCGCCGGGCGCGCTGTCAGAGCACTATAAACGCGGGG |
| mm4_intron_Ppcdc_chr9_57417147 |
TCCCGGGGATACAGCAACTATGACACCCCTGCTCAGAAAGAGTCCCGAGTCCCGAGTGCCCCTGCCCCCTCCTGGGACAG CAAACAGTCTGGAATCTGGTTGCTTGCCTCGTCCCAGGCATCAACAT |
| mm4_intergenic_Ppp1r37|Gemin7_chr7_19563866 |
TAACCACCAACCTGCAGCATTTGCTCCCGGGCACTGGCACGGCACTGGCACACAAGTGTGACCACCACCACCACTCCCAG TCCCCTCCGTGGGAAGACAAACCTGCAGGTGACTGCAGTGGACCACATCACCAAGAGGG |
| mm4_intergenic_G930045G22Rik|Mir148a_chr6_50912362 |
TCCAAGGCTCACGAGTTCACAAAGCTGTATTGTCCACAGTCACCTCCCCCGAGAGCCCATGGCCAGGGATGGTGTGCTGT GTAGCCTAAAAGGGCAGCAGTTTGATCACA |
| mm4_intron_Epb4.1_chr4_131976623 |
CCGGCCTTACACTTGCTAGTGTACCTCTCAGCAACCCTCCTGTAACCCGCAGTCACCACGGTGGACAGCGAGTCTCTGCG ACTTACTTCCTCCTCCTGCCAGGCCTACTAGA |
| mm4_intergenic_Adat2|Aig1_chr10_13566726 |
GGCCACCTTTCTTGAGAGCTCTCCCACCCAGCCACACCCCCCTCCACGGACAGTTTTTCATACCTGTCTTGCCCCTATCA CC |
| mm4_intron_Zfhx3_chr8_108900931 |
GCTTATTAGCGGAAGGGGAAAAACAATTACTTGACTTTGCCGGGCTCTCCGCGGCTGGGACTCGGGCCTGTGGCAGCAAC CCTGCCACCCGAAGCCTTGGACTTACCCCGGACCTAG |
| mm4_intergenic_Gm16045|Sept9_chr11_117266133 |
CATCCTCTACATCCGCCCACATCTGGCCGCGGAGGGGCGTGAGGGGGAGGGGCCGAGGCCGTGTCTGTCGCGGTCCCCTG GGCACCCGCCAGGAAGGGAGAAGGGGGGGCAGCCCAGGTCGTGTGCCTGGGACTCTGCAG |
| mm4_intergenic_Slc6a13|Gm24855_chr6_121325173 |
ACTTCTGCATCTGGAAGGGGGTCAAGTCCACGGGCAAGGTCTGTGCTGGCGAGCTCCGCTCTCCCCTCCCAGGACAGCCT GCCTCTCCTAAGGGCTGCCACTCCTGCTTTAGGCTGGTCCAGGCAG |
| mm4_exon_Efcab7_chr4_99901764 | AGTGTGATGAGGATGCCTGGGCTGTCTGCAGAGGTGAGTGTGGCATCCCTCATTCTCAGAAACAAGCC |
| mm4_exon_Cetn4_chr3_37312397 |
TCACCATATCCCCTCCCTGCGCCCCTGCCCGCGCCCGCGCAACAGCCGCTCTCCCCTCCGCGGCCCGCGTTTGCTCCCGA CAGGCGGTTGCTATAGCAGCGCTCAGCGCGCAAGCCCGCCCACCAGCTTCCGTGATTGA |
| mm4_intron_Wdfy3_chr5_101934818 |
CTCCTCACAAACCCCAGCTGGCGATGTTTGTATCAGGGTTCAGGATCTGCCACCGTAGCCTCCGAGGACAGCCAAGGACA GAGGCGAGCACTCAAT |
| mm4_intron_Gm4779_chrX_101791737 |
TGGAAGGGACTAATGGGGTCCAAAATGAGGCCGCAGTGCCCTCTGCTGGCAGCACCTTGTACCTGGTAAGTCTTCACCTC TATCTAGCTCTATTCTTGCCCTTACTGCAGCCCAGAGGCAGCATAAAGGT |
| mm4_intron_Fam53b_chr7_132811110 |
TAAAGAGCTCTGCAGGCACCAGATTGCTCCACAGTGCCCCCTGCTGACAGCATTTAGACACAGCTCTCTCCTGGAGGAGG AGGAGAGCTAGCTTTGGCTGAGCTCCCATCA |
| mm4_intron_Dym_chr18_75119541 |
TGAGCAGGTTCTCCAAGCAGCATCCTCTGTACCCACCGAGGACAGCTGTATTTCTTCATGTTCCTTCTAGCTGATGAGGG CTGTCCACCGCTCTGTTGGTCATGATTGCCACACAGC |
| mm4_intron_Adcy5_chr16_35267311 |
ACCCTCAATGAGCTCTTCGCCCGCTTTGACAAGTTGGCTGCGGTGAGTCACCTTGAGTGCGGGCTGTCAGGGGAGGGCCC TGAGGTTGAGGACGGTAATCTAGGTTTTTACTCTGCTTAGGGGAGACCGGTGTTG |
| mm4_intron_9530036O11Rik_chr5_28732628 | ATGTGGTCTGCTCTGTGGTGCCCCAGTGCCTTCCGTGCACAGCTAGCACCTCCCTTTCCCACATCGTC |
| mm4_intergenic_Gm13056|Epha2_chr4_141297476 |
TCAGGAGGACTCAGAGGCTTAGGAAACGACCTGTCCCCGGAAGGCACTGAGGGACAGTCATTTCAGCGACCTGGAAGTTC TGGTTGCAGAGAGCAGATCCGTCATTCCAGGTGGAGGGGCTGTGGGATTCAGAGGCAGG |
| mm4_intergenic_Dlc1|AI429214_chr8_36964067 |
TCTGTGTGCCTTGAGTGGACAAGGGAGCAGAGGCTGGACTGCTCAACTAACTTGTCAGTGTTTGTCCGGGGAGGGTACTG TGGACCATTAGCATCAGGCTCCTGTCCTTCATGGGTT |