CRISPOR

DNA break repair outcome predictions

Guide: AGGCTGTCCGCGGAGGGGAC TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TAGGTTGCCCTTGGCCAGAGAGGCTGTCCGCGGAGGGGACTGGGGGTCCCGGGCCAAGCATGGCCGCTCCAGCAGACCGT`	(Wild-type sequence)
564	`TTGGCCAGAGAGGCTGTCCGCGGAGGGG-------GTCCCGGGCCAAGCATGGCCGCTCC`	`7 bp deleted → frameshift`
536	`TTGGCCAGAGAGGCTGTCCGCGGAGGGG--------TCCCGGGCCAAGCATGGCCGCTCC`	`8 bp deleted → frameshift`
444	`TTGGCCAGAGAGGCTGTCCGCGGAGGGG------GGTCCCGGGCCAAGCATGGCCGCTCC`	`6 bp deleted → no frameshift`
382	`TTGGCCAGAGAGGCTGTCCGCGGAGGG---------TCCCGGGCCAAGCATGGCCGCTCC`	`9 bp deleted → no frameshift`
311	`TTGGCCAGAGAGGCTGTCCGCGGAGGGG-----GGGTCCCGGGCCAAGCATGGCCGCTCC`	`5 bp deleted → frameshift`
283	`TTGGCCAGAGAGGCTGTCCGCGGAGGGG---------------CCAAGCATGGCCGCTCC`	`15 bp deleted → no frameshift`
269	`TTGGCCAGAGAGGCTGTCCGCGGAGGG----------------CCAAGCATGGCCGCTCC`	`16 bp deleted → frameshift`
257	`TTGGCCAGAGAGGCTGTCC--------------------CGGGCCAAGCATGGCCGCTCC`	`20 bp deleted → frameshift`
242	`TTGGCCAGAGAGGCTGTCCGCGGAGG----------TCCCGGGCCAAGCATGGCCGCTCC`	`10 bp deleted → frameshift`
242	`TTGGCCAGAGAGGCTGTCCGCGG----------GGGTCCCGGGCCAAGCATGGCCGCTCC`	`10 bp deleted → frameshift`
232	`TTGGCCAGAGAGGCTGTCCGCGG-------------------GCCAAGCATGGCCGCTCC`	`19 bp deleted → frameshift`
230	`TTGGCCAGAGAGGCTGTCCGCGG-----------GGTCCCGGGCCAAGCATGGCCGCTCC`	`11 bp deleted → frameshift`
219	`TTGGCCAGAGAGGCTGTCCGCGG------------GTCCCGGGCCAAGCATGGCCGCTCC`	`12 bp deleted → no frameshift`
210	`TTGGCCAGAGAGGCTGTCCG---------------------GGCCAAGCATGGCCGCTCC`	`21 bp deleted → no frameshift`
208	`TTGGCCAGAGAGGCTGTCCGCGG-------------TCCCGGGCCAAGCATGGCCGCTCC`	`13 bp deleted → frameshift`
198	`TTGGCCAGAGAGGCTGTCCGCGGAGGGG--------------GCCAAGCATGGCCGCTCC`	`14 bp deleted → frameshift`
170	`TTGGCCAGAGAGGCTGTCCGCGGAGG-----------------CCAAGCATGGCCGCTCC`	`17 bp deleted → frameshift`
147	`TTGGCCAGAGAGGCTGTCCGCGG--------------------CCAAGCATGGCCGCTCC`	`20 bp deleted → frameshift`
147	`TTGGCCAGAGAGG--------------------GGGTCCCGGGCCAAGCATGGCCGCTCC`	`20 bp deleted → frameshift`
140	`TTGGCCAGAGAGG---------------------GGTCCCGGGCCAAGCATGGCCGCTCC`	`21 bp deleted → no frameshift`
134	`TTGGCCAGAGAGGCTG----------------GGGGTCCCGGGCCAAGCATGGCCGCTCC`	`16 bp deleted → frameshift`
133	`TTGGCCAGAGAGGC------------------------------CAAGCATGGCCGCTCC`	`30 bp deleted → no frameshift`
133	`TTGGCCAGAGAGG----------------------GTCCCGGGCCAAGCATGGCCGCTCC`	`22 bp deleted → frameshift`
132	`TTGGCCAGAGAGGCTGTCCGC------------------------------------TCC`	`36 bp deleted → no frameshift`
127	`TTGGCCA---------------------------------------AGCATGGCCGCTCC`	`39 bp deleted → no frameshift`
126	`TTGGCCAGAGAGGCTGTCCGC-----------------------CAAGCATGGCCGCTCC`	`23 bp deleted → frameshift`
126	`TTGGCCAGAGAGG-----------------------TCCCGGGCCAAGCATGGCCGCTCC`	`23 bp deleted → frameshift`
117	`TTGG-----------------------------GGGTCCCGGGCCAAGCATGGCCGCTCC`	`29 bp deleted → frameshift`
114	`TTGGCCAGAGAGGCTGTCCGCGGAGGGG-------------------------CCGCTCC`	`25 bp deleted → frameshift`
109	`TTGGCCAGAGAGGCTGTCCGCGGAGGG--------------------------CCGCTCC`	`26 bp deleted → frameshift`
109	`TTGGCCAGAGAGGCTGTCC--------------------------AAGCATGGCCGCTCC`	`26 bp deleted → frameshift`
103	`TTGGCCAGAGAGGCTGTCCGCGGAGG---------------------------CCGCTCC`	`27 bp deleted → no frameshift`
98	`TTGGCCAGAGAGGCTGTCCGC----------------------------ATGGCCGCTCC`	`28 bp deleted → frameshift`
95	`TTGGCCAGAGAGGCTGTCCGCGGAG-----------------------CATGGCCGCTCC`	`23 bp deleted → frameshift`
94	`TTGGCCAGAGAGG-----------------------------GCCAAGCATGGCCGCTCC`	`29 bp deleted → frameshift`
89	`TTGGCCAGAGAGGCTGTCCGCGG------------------------------CCGCTCC`	`30 bp deleted → no frameshift`
89	`TTGG------------------------------GGTCCCGGGCCAAGCATGGCCGCTCC`	`30 bp deleted → no frameshift`
84	`TTGG-------------------------------GTCCCGGGCCAAGCATGGCCGCTCC`	`31 bp deleted → frameshift`
81	`TTGGCCAGAGAGGC----------------------------------------CGCTCC`	`40 bp deleted → frameshift`
80	`TTGG--------------------------------TCCCGGGCCAAGCATGGCCGCTCC`	`32 bp deleted → frameshift`
77	`TTGGCC-------------------------------------------------GCTCC`	`49 bp deleted → frameshift`
76	`TTGGCCAGAGAGGCTGTCCGC---------------------------------CGCTCC`	`33 bp deleted → no frameshift`
76	`TTGGCC---------------------------------CGGGCCAAGCATGGCCGCTCC`	`33 bp deleted → no frameshift`
73	`TTGGCCAGAGAGGCTGTCCGCG----------------------------------CTCC`	`34 bp deleted → frameshift`
73	`TTGGCC----------------------------------GGGCCAAGCATGGCCGCTCC`	`34 bp deleted → frameshift`
69	`TTGGCCAGAGAGGC-----------------------------------ATGGCCGCTCC`	`35 bp deleted → frameshift`
64	`TTGGCCAGAGAGGCTGTCC-----------------------------------------`	`41 bp deleted → frameshift`
60	`TTGG--------------------------------------GCCAAGCATGGCCGCTCC`	`38 bp deleted → frameshift`
58	`TTGGCCAGAGAGGCT-------------------------------------------CC`	`43 bp deleted → frameshift`
49	`TTGGCCAGAGAGGCTG------------------------------------GCCGCTCC`	`36 bp deleted → no frameshift`
49	`TTGGCCAGAGAG------------------------------------CATGGCCGCTCC`	`36 bp deleted → no frameshift`
45	`TTGGCCAGAG--------------------------------------CATGGCCGCTCC`	`38 bp deleted → frameshift`
44	`TTGGC--------------------------------------------ATGGCCGCTCC`	`44 bp deleted → frameshift`
40	`TTGGCCAG----------------------------------------CATGGCCGCTCC`	`40 bp deleted → frameshift`
34	`TTGGCCA-------------------------------------------TGGCCGCTCC`	`43 bp deleted → frameshift`
29	`TTGGC----------------------------------------------------TCC`	`52 bp deleted → frameshift`
26	`TTGGCC------------------------------------------------------`	`54 bp deleted → no frameshift`

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