CRISPOR

DNA break repair outcome predictions

Guide: CGTTCCCGGTGGCGCAGTCT GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TTTGCTGTGCTGGTACTCGGCGTTCCCGGTGGCGCAGTCTGGGGTGCAGCTCACCTCGATTTCTTCATAGAAATCCGATT`	(Wild-type sequence)
384	`TGGTACTCGGCGTTCCCGGTGGCGCAG------------CTCACCTCGATTTCTTCATAG`	`12 bp deleted → no frameshift`
330	`TGGTACTCGGCGTTCCCGGTG---------------CAGCTCACCTCGATTTCTTCATAG`	`15 bp deleted → no frameshift`
242	`TGGTACTCGGCGTTCCCGGTGG----------GGTGCAGCTCACCTCGATTTCTTCATAG`	`10 bp deleted → frameshift`
230	`TGGTACTCGGCGTTCCCGGTGG-----------GTGCAGCTCACCTCGATTTCTTCATAG`	`11 bp deleted → frameshift`
219	`TGGTACTCGGCGTTCCCGGTGG------------TGCAGCTCACCTCGATTTCTTCATAG`	`12 bp deleted → no frameshift`
211	`TGGTACTCGGCGTTCCCGGTGGCGCAGT-------GCAGCTCACCTCGATTTCTTCATAG`	`7 bp deleted → frameshift`
208	`TGGTACTCGGCGTTCCCGG-------------GGTGCAGCTCACCTCGATTTCTTCATAG`	`13 bp deleted → frameshift`
198	`TGGTACTCGGCGTTCCCGGTGGC--------------AGCTCACCTCGATTTCTTCATAG`	`14 bp deleted → frameshift`
198	`TGGTACTCGGCGTTCCCGG--------------GTGCAGCTCACCTCGATTTCTTCATAG`	`14 bp deleted → frameshift`
188	`TGGTACTCGGCGTTCCCGGTGGCGC---------------TCACCTCGATTTCTTCATAG`	`15 bp deleted → no frameshift`
173	`TGGTACTCGGCGTTCCCGGTGGCGCAGTCT-----------CACCTCGATTTCTTCATAG`	`11 bp deleted → frameshift`
170	`TGGTACTCGGCGTTCCCGGTGGC-----------------TCACCTCGATTTCTTCATAG`	`17 bp deleted → frameshift`
156	`TGGTACTCGGCGTTCCCGGTGGCGCAGTC-------------ACCTCGATTTCTTCATAG`	`13 bp deleted → frameshift`
134	`TGGTACTCGGCGTTCCCGGTGGCGCAGTCT----------------CGATTTCTTCATAG`	`16 bp deleted → frameshift`
133	`TGGTACTCGG----------------------GGTGCAGCTCACCTCGATTTCTTCATAG`	`22 bp deleted → frameshift`
128	`TGGTACTCGGCGTTCCCGGTGGCGCA-----------------CCTCGATTTCTTCATAG`	`17 bp deleted → frameshift`
126	`TGGTACTCGG-----------------------GTGCAGCTCACCTCGATTTCTTCATAG`	`23 bp deleted → frameshift`
122	`TGGTACTCGGCGTTCCCGGTGGCGCAGTC------------------GATTTCTTCATAG`	`18 bp deleted → no frameshift`
120	`TGGTACTCGGCGTTCCCGGTGGCGCAGTCT------------------------TCATAG`	`24 bp deleted → no frameshift`
120	`TGGTACTCGGCGTTCCCGGTGGCG------------------------ATTTCTTCATAG`	`24 bp deleted → no frameshift`
120	`TGGTACTCGG------------------------TGCAGCTCACCTCGATTTCTTCATAG`	`24 bp deleted → no frameshift`
109	`TGGTACTCGGC--------------------------AGCTCACCTCGATTTCTTCATAG`	`26 bp deleted → frameshift`
106	`TGGT-------------------------------GCAGCTCACCTCGATTTCTTCATAG`	`31 bp deleted → frameshift`
99	`TGGTACTCGGCGT----------------------GCAGCTCACCTCGATTTCTTCATAG`	`22 bp deleted → frameshift`
99	`TGGTACTCG---------------------------------------ATTTCTTCATAG`	`39 bp deleted → no frameshift`
98	`TGGTACTCGGCGTTCCC----------------------------TCGATTTCTTCATAG`	`28 bp deleted → frameshift`
94	`TGGTACTCGGCGTTCC-----------------------------TCGATTTCTTCATAG`	`29 bp deleted → frameshift`
94	`TGGTACTCGGC-----------------------------TCACCTCGATTTCTTCATAG`	`29 bp deleted → frameshift`
94	`TGG-----------------------------GGTGCAGCTCACCTCGATTTCTTCATAG`	`29 bp deleted → frameshift`
91	`TGGTACTC----------------------------------ACCTCGATTTCTTCATAG`	`34 bp deleted → frameshift`
89	`TGGTACTCGGCGTTCCCG------------------------------ATTTCTTCATAG`	`30 bp deleted → no frameshift`
89	`TGG------------------------------GTGCAGCTCACCTCGATTTCTTCATAG`	`30 bp deleted → no frameshift`
77	`TGGTACTCGGCGTTCCCGGTGGCGCAGTC---------------------------ATAG`	`27 bp deleted → no frameshift`
77	`TGGTACTCGGCGTTC---------------------------ACCTCGATTTCTTCATAG`	`27 bp deleted → no frameshift`
66	`TGGTACTCGGCG------------------------------------ATTTCTTCATAG`	`36 bp deleted → no frameshift`
63	`TGGTACTCGGCGTTCCCGGTGGCGCA-------------------------------TAG`	`31 bp deleted → frameshift`
60	`TGGTACTCGGCGTTC--------------------------------GATTTCTTCATAG`	`32 bp deleted → frameshift`
60	`TGGTACTCGGCGTTC--------------------------------------TTCATAG`	`38 bp deleted → frameshift`
57	`TGGTACTCGGCGTTCCCGGTGGCGCAG---------------------------------`	`33 bp deleted → no frameshift`
54	`TG----------------------------------CAGCTCACCTCGATTTCTTCATAG`	`34 bp deleted → frameshift`
51	`TGGTACTCGGCGTTC-----------------------------------------ATAG`	`41 bp deleted → frameshift`
45	`TGGTAC--------------------------------------CTCGATTTCTTCATAG`	`38 bp deleted → frameshift`
31	`TGGTACTC---------------------------------------------TTCATAG`	`45 bp deleted → no frameshift`
31	`TGGTACTCGGCGTT-------------------------------------TCTTCATAG`	`37 bp deleted → frameshift`
28	`TGGTACT-----------------------------------------------TCATAG`	`47 bp deleted → frameshift`
27	`TGGTACTC------------------------------------------------ATAG`	`48 bp deleted → no frameshift`
13	`TGGTA------------------------------------------------------G`	`54 bp deleted → no frameshift`

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