CRISPOR

DNA break repair outcome predictions

Guide: CACGCTCCCGGGAAGTCGGA GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CTCCTCCTGGCCTCCCCCGCCACGCTCCCGGGAAGTCGGAGGGCCGGCTCGCGGGCTCAGGCAACCGTGGGGCACCATCT`	(Wild-type sequence)
402	`CCTCCCCCGCCACGCTCCCGGGAAGTCGG--------CTCGCGGGCTCAGGCAACCGTGG`	`8 bp deleted → frameshift`
359	`CCTCCCCCGCCACGCTCCCGG----------------CTCGCGGGCTCAGGCAACCGTGG`	`16 bp deleted → frameshift`
346	`CCTCCCCCGCCACGCTCCCGGG-----------CCGGCTCGCGGGCTCAGGCAACCGTGG`	`11 bp deleted → frameshift`
344	`CCTCCCCCGCCACGCTCCCGGGAAGTCGG---GCCGGCTCGCGGGCTCAGGCAACCGTGG`	`3 bp deleted → no frameshift`
327	`CCTCCCCCGCCACGCTCCCGGGAAGTCGG----CCGGCTCGCGGGCTCAGGCAACCGTGG`	`4 bp deleted → frameshift`
283	`CCTCCCCCGCCACGCTCCCGGGAAGTCGG---------------GCTCAGGCAACCGTGG`	`15 bp deleted → no frameshift`
261	`CCTCCCCCGCCACGCTCCCGGGAAGTCG-------------CGGGCTCAGGCAACCGTGG`	`13 bp deleted → frameshift`
253	`CCTCCCCCGCCACGCTCCCGGG-----------------------CTCAGGCAACCGTGG`	`23 bp deleted → frameshift`
242	`CCTCCCCCGCCACGCTCCCGGG----------GCCGGCTCGCGGGCTCAGGCAACCGTGG`	`10 bp deleted → frameshift`
221	`CCTCCCCCGCCACGCTC-----------------------GCGGGCTCAGGCAACCGTGG`	`23 bp deleted → frameshift`
219	`CCTCCCCCGCCACGCTCCCGG------------CCGGCTCGCGGGCTCAGGCAACCGTGG`	`12 bp deleted → no frameshift`
188	`CCTCCCCCGCCACGCTCCCGGG---------------CTCGCGGGCTCAGGCAACCGTGG`	`15 bp deleted → no frameshift`
180	`CCTCCCCCGCC------------------------GGCTCGCGGGCTCAGGCAACCGTGG`	`24 bp deleted → no frameshift`
179	`CCTCCCCCGCCACGCTCCCGGGAAGTCGG----------------CTCAGGCAACCGTGG`	`16 bp deleted → frameshift`
170	`CCTCCCCCGCCACGCTCC-----------------GGCTCGCGGGCTCAGGCAACCGTGG`	`17 bp deleted → frameshift`
155	`CCTCCCCCGCCACGC---------------------------GGGCTCAGGCAACCGTGG`	`27 bp deleted → no frameshift`
155	`CCTCCCCCG---------------------------GCTCGCGGGCTCAGGCAACCGTGG`	`27 bp deleted → no frameshift`
154	`CCTCCCCCGCCACGC-------------------CGGCTCGCGGGCTCAGGCAACCGTGG`	`19 bp deleted → frameshift`
148	`CCTCCCCCGCCACGCTC-------------------------------AGGCAACCGTGG`	`31 bp deleted → frameshift`
140	`CCTCCCCCGCCACGCTCCCG---------------------CGGGCTCAGGCAACCGTGG`	`21 bp deleted → no frameshift`
133	`CCTCCCCCGCCACGCTCCCGGGAAGTCGG----------------------CAACCGTGG`	`22 bp deleted → frameshift`
133	`CCTCCCCCGCCACGCTCCCGGG----------------------GCTCAGGCAACCGTGG`	`22 bp deleted → frameshift`
133	`CCTCCCCCGCCACG----------------------GCTCGCGGGCTCAGGCAACCGTGG`	`22 bp deleted → frameshift`
121	`CCTCCCCCGC--------------------------------GGGCTCAGGCAACCGTGG`	`32 bp deleted → frameshift`
120	`CCTCCCCCGCCACGCTCCCGG------------------------CTCAGGCAACCGTGG`	`24 bp deleted → no frameshift`
105	`CCTCCCCCGCCACGCTCCCGGGAAGTC---------------------AGGCAACCGTGG`	`21 bp deleted → no frameshift`
98	`CCTCCCCCGC----------------------------TCGCGGGCTCAGGCAACCGTGG`	`28 bp deleted → frameshift`
98	`CCTCCCC----------------------------GGCTCGCGGGCTCAGGCAACCGTGG`	`28 bp deleted → frameshift`
94	`CCTCCCCCGCCACGCTCCCG-------------------------------------TGG`	`37 bp deleted → frameshift`
94	`CCTCCCCCGCCACGCTCCCGGGAAGTCG-----------------------------TGG`	`29 bp deleted → frameshift`
94	`CCTCCCCCGCCACGCTCCCGGG-----------------------------CAACCGTGG`	`29 bp deleted → frameshift`
94	`CCTCCCCCGCCACG-----------------------------GGCTCAGGCAACCGTGG`	`29 bp deleted → frameshift`
94	`CCTCCC-----------------------------GGCTCGCGGGCTCAGGCAACCGTGG`	`29 bp deleted → frameshift`
89	`CCTCCCCCGCCACGCTCCCGG------------------------------CAACCGTGG`	`30 bp deleted → no frameshift`
89	`CCTCC------------------------------GGCTCGCGGGCTCAGGCAACCGTGG`	`30 bp deleted → no frameshift`
86	`CCTCCCCCGCCACGCTCCCGGGAAG-------------------------GCAACCGTGG`	`25 bp deleted → frameshift`
84	`CCTCCCCCGCCACGCTCCCGGGAAGTCGG-------------------------------`	`31 bp deleted → frameshift`
82	`CCTC------------------------------------GCGGGCTCAGGCAACCGTGG`	`36 bp deleted → no frameshift`
76	`CC---------------------------------GGCTCGCGGGCTCAGGCAACCGTGG`	`33 bp deleted → no frameshift`
73	`CCTCCCCCG----------------------------------GGCTCAGGCAACCGTGG`	`34 bp deleted → frameshift`
66	`CCTCCCCCGC------------------------------------TCAGGCAACCGTGG`	`36 bp deleted → no frameshift`
62	`CCTCCCCCGCCACGC-------------------------------------AACCGTGG`	`37 bp deleted → frameshift`
60	`CCTCCCCCGCCACGCTCCCGGGAAGT--------------------------------GG`	`32 bp deleted → frameshift`
60	`CCTCCCCCGCCACGCTCCCGGG--------------------------------------`	`38 bp deleted → frameshift`
60	`CCTCCCCCGCCACGCTCC--------------------------------------GTGG`	`38 bp deleted → frameshift`
56	`CCTCCCCCGCCACGCTCCCGG---------------------------------------`	`39 bp deleted → no frameshift`
55	`CCTC--------------------------------------------AGGCAACCGTGG`	`44 bp deleted → frameshift`
54	`CCTCCCCCG------------------------------------------------TGG`	`48 bp deleted → no frameshift`
48	`CCTCCCCCGC------------------------------------------AACCGTGG`	`42 bp deleted → no frameshift`
47	`CCTCCCCCGCCA-------------------------------------GGCAACCGTGG`	`37 bp deleted → frameshift`
46	`CCTCCCCCGCCACG-------------------------------------------TGG`	`43 bp deleted → frameshift`
44	`CCTCCCCCGCCACGCTCCCGGGAA------------------------------CCGTGG`	`30 bp deleted → no frameshift`
42	`CCTCCCCCGCC---------------------------------------------GTGG`	`45 bp deleted → no frameshift`
38	`CCTCCCCCGCCA-----------------------------------------ACCGTGG`	`41 bp deleted → frameshift`
36	`CCTCCCCCGCCAC------------------------------------------CGTGG`	`42 bp deleted → no frameshift`
34	`CCTCCCC-------------------------------------------------GTGG`	`49 bp deleted → frameshift`
32	`CCTCCC--------------------------------------------------GTGG`	`50 bp deleted → frameshift`
31	`CCTCC---------------------------------------------------GTGG`	`51 bp deleted → no frameshift`
26	`CC------------------------------------------------------GTGG`	`54 bp deleted → no frameshift`

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