CRISPOR

DNA break repair outcome predictions

Guide: CGACTTCCCGGGAGCGTGGC GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GAGCCCGCGAGCCGGCCCTCCGACTTCCCGGGAGCGTGGCGGGGGAGGCCAGGAGGAGGAGCCGGTCGATGGACTAGCAG`	(Wild-type sequence)
469	`GCCGGCCCTCCGACTTCCCGGGAG-------------GCCAGGAGGAGGAGCCGGTCGAT`	`13 bp deleted → frameshift`
382	`GCCGGCCCTCCGACTTCCCGGGAGCGTGGC---------CAGGAGGAGGAGCCGGTCGAT`	`9 bp deleted → no frameshift`
346	`GCCGGCCCTCCGACTTCCCGGG-----------GGAGGCCAGGAGGAGGAGCCGGTCGAT`	`11 bp deleted → frameshift`
344	`GCCGGCCCTCCGACTTCCCGGGAGCGTGG---GGGAGGCCAGGAGGAGGAGCCGGTCGAT`	`3 bp deleted → no frameshift`
329	`GCCGGCCCTCCGACTTCCCGGG------------GAGGCCAGGAGGAGGAGCCGGTCGAT`	`12 bp deleted → no frameshift`
327	`GCCGGCCCTCCGACTTCCCGGGAGCGTGG----GGAGGCCAGGAGGAGGAGCCGGTCGAT`	`4 bp deleted → frameshift`
311	`GCCGGCCCTCCGACTTCCCGGGAGCGTGG-----GAGGCCAGGAGGAGGAGCCGGTCGAT`	`5 bp deleted → frameshift`
296	`GCCGGCCCTCCGACTTCCCGGGAGCGTGG------AGGCCAGGAGGAGGAGCCGGTCGAT`	`6 bp deleted → no frameshift`
245	`GCCGGCCCTCCGACTTCCCGGGAG---------------------GAGGAGCCGGTCGAT`	`21 bp deleted → no frameshift`
242	`GCCGGCCCTCCGACTTCCCGGG----------GGGAGGCCAGGAGGAGGAGCCGGTCGAT`	`10 bp deleted → frameshift`
233	`GCCGGCCCTCCGACTTCCCGGGAGC---------------------------CGGTCGAT`	`27 bp deleted → no frameshift`
210	`GCCGGCCCTCCGACTTCCCGGGAG------------------------GAGCCGGTCGAT`	`24 bp deleted → no frameshift`
198	`GCCGGCCCTCCGACTTCCCGGGAGCGTGG--------------AGGAGGAGCCGGTCGAT`	`14 bp deleted → frameshift`
198	`GCCGGCCCTCCGACTTCCCGGGAGC--------------CAGGAGGAGGAGCCGGTCGAT`	`14 bp deleted → frameshift`
198	`GCCGGCCCTCCGACTTCCCGG--------------AGGCCAGGAGGAGGAGCCGGTCGAT`	`14 bp deleted → frameshift`
179	`GCCGGCCCTCCGACTTCCCGGG----------------CCAGGAGGAGGAGCCGGTCGAT`	`16 bp deleted → frameshift`
170	`GCCGGCCCTCCGACTTCCCGGGAGCGTGG-----------------AGGAGCCGGTCGAT`	`17 bp deleted → frameshift`
170	`GCCGGCCCTCCGACTTCCCGG-----------------CCAGGAGGAGGAGCCGGTCGAT`	`17 bp deleted → frameshift`
153	`GCCGGCC---------------------------------AGGAGGAGGAGCCGGTCGAT`	`33 bp deleted → no frameshift`
147	`GCCGGCCCTCCGACTTCCCGGGAGCGTGG--------------------AGCCGGTCGAT`	`20 bp deleted → frameshift`
146	`GCCGGCCCTCCGACTTCCCGG----------------------------------TCGAT`	`34 bp deleted → frameshift`
140	`GCCGGCCCTCCGACTTCCC---------------------AGGAGGAGGAGCCGGTCGAT`	`21 bp deleted → no frameshift`
133	`GCCGGCCCTCCGACTTCCCGGGAGCGTGGC----------------------CGGTCGAT`	`22 bp deleted → frameshift`
133	`GCCGGCCCTCCGACTTCCCGG----------------------AGGAGGAGCCGGTCGAT`	`22 bp deleted → frameshift`
133	`GCCGGCCCTCCGACTTCC----------------------AGGAGGAGGAGCCGGTCGAT`	`22 bp deleted → frameshift`
122	`GCCGGCCCTCCGACTTCCCGGGAG------------------GAGGAGGAGCCGGTCGAT`	`18 bp deleted → no frameshift`
114	`GCCGGCCCTCCGACTTCCCGG-------------------------AGGAGCCGGTCGAT`	`25 bp deleted → frameshift`
109	`GCCGGCCCTCCGACTTCCCGGGAGCGTGG--------------------------TCGAT`	`26 bp deleted → frameshift`
103	`GCCGG---------------------------GGGAGGCCAGGAGGAGGAGCCGGTCGAT`	`27 bp deleted → no frameshift`
98	`GCCGGCCCTCCGACTTCCCGGGAGCG----------------------------GTCGAT`	`28 bp deleted → frameshift`
98	`GCCGGCCCTCCGACTTCCCGG----------------------------AGCCGGTCGAT`	`28 bp deleted → frameshift`
98	`GCCGG----------------------------GGAGGCCAGGAGGAGGAGCCGGTCGAT`	`28 bp deleted → frameshift`
95	`GCCGGCCCTCCGA-----------------------GGCCAGGAGGAGGAGCCGGTCGAT`	`23 bp deleted → frameshift`
94	`GCC-------------------------------------AGGAGGAGGAGCCGGTCGAT`	`37 bp deleted → frameshift`
94	`GCCGGCCCTCC-----------------------------AGGAGGAGGAGCCGGTCGAT`	`29 bp deleted → frameshift`
94	`GCCGG-----------------------------GAGGCCAGGAGGAGGAGCCGGTCGAT`	`29 bp deleted → frameshift`
89	`GCCGG------------------------------AGGCCAGGAGGAGGAGCCGGTCGAT`	`30 bp deleted → no frameshift`
82	`GCCGG--------------------------------------------------TCGAT`	`50 bp deleted → frameshift`
80	`GCCGGCCCTCCGACTTCCCGGGAGCG--------------------------------AT`	`32 bp deleted → frameshift`
80	`GCCGGCCC--------------------------------AGGAGGAGGAGCCGGTCGAT`	`32 bp deleted → frameshift`
76	`GCCGGCCCTCCGACTTCCCGGG---------------------------------TCGAT`	`33 bp deleted → no frameshift`
73	`GCCGGCCCTCCG------------------------------------------GTCGAT`	`42 bp deleted → no frameshift`
70	`GCCGGCCCTCCGACTTCCCGGGAGCGT-----------------------------CGAT`	`29 bp deleted → frameshift`
69	`GCCGGCCCTCCGACTTCC-----------------------------------GGTCGAT`	`35 bp deleted → frameshift`
63	`GCCGGCCCTCCGA-------------------------------GGAGGAGCCGGTCGAT`	`31 bp deleted → frameshift`
60	`GCCGGCCCTCCGACTTCCCG--------------------------------------AT`	`38 bp deleted → frameshift`
60	`GCCGGCC----------------------------------------------GGTCGAT`	`46 bp deleted → frameshift`
60	`GCCGG--------------------------------------AGGAGGAGCCGGTCGAT`	`38 bp deleted → frameshift`
54	`GCCGGCCCTCCGA----------------------------------GGAGCCGGTCGAT`	`34 bp deleted → frameshift`
51	`GCCGG-----------------------------------------AGGAGCCGGTCGAT`	`41 bp deleted → frameshift`
50	`GCCGGCCCTCCGA----------------------------------------------T`	`46 bp deleted → frameshift`
49	`GCCGGCCCTCCGACTTCCCGGGA------------------------------------T`	`36 bp deleted → no frameshift`
47	`GCCGGCCCTCCGA-------------------------------------GCCGGTCGAT`	`37 bp deleted → frameshift`
44	`GCCGG--------------------------------------------AGCCGGTCGAT`	`44 bp deleted → frameshift`
42	`GCCGGCCC---------------------------------------------GGTCGAT`	`45 bp deleted → no frameshift`
40	`GCCGGCCCTCCGACTTC----------------------------------------GAT`	`40 bp deleted → frameshift`
28	`GCCGGCCCTC-----------------------------------------------GAT`	`47 bp deleted → frameshift`
26	`GCCG------------------------------------------------------AT`	`54 bp deleted → no frameshift`

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