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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313821_F | Primer3: not found at this Tm | N.d. | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313821_R | Primer3: not found at this Tm | N.d. | 1.00 |
| mm4_exon_Fam181b/Gm9934_chr7_93081075_F | TCGTCGGCAGCGTCTGACCTTGAACGAGCCTGTG | 60.2 | 0.63 |
| mm4_exon_Fam181b/Gm9934_chr7_93081075_R | GTCTCGTGGGCTCGGCATAGGACACCTGATGGGGC | 59.8 | 0.63 |
| mm4_intron_Lypd6b_chr2_49866800_F | Primer3: not found at this Tm | N.d. | 0.60 |
| mm4_intron_Lypd6b_chr2_49866800_R | Primer3: not found at this Tm | N.d. | 0.60 |
| mm4_intron_Spon1_chr7_113894975_F | Primer3: not found at this Tm | N.d. | 0.59 |
| mm4_intron_Spon1_chr7_113894975_R | Primer3: not found at this Tm | N.d. | 0.59 |
| mm3_intron_Sufu_chr19_46477778_F | TCGTCGGCAGCGTCAGTTGCTGTGCTAGGAGCTG | 60.0 | 0.52 |
| mm3_intron_Sufu_chr19_46477778_R | GTCTCGTGGGCTCGGCTTGGTGATCAAGGGTGCGT | 60.6 | 0.52 |
| mm4_intron_Btbd16_chr7_130797862_F | TCGTCGGCAGCGTCTGCTTCTGGCTTTGACCCTT | 59.8 | 0.49 |
| mm4_intron_Btbd16_chr7_130797862_R | GTCTCGTGGGCTCGGAGGAGGACACATGCACAGTG | 59.9 | 0.49 |
| mm4_intergenic_Gm12722|Gm12723_chr4_105438044_F | Primer3: not found at this Tm | N.d. | 0.46 |
| mm4_intergenic_Gm12722|Gm12723_chr4_105438044_R | Primer3: not found at this Tm | N.d. | 0.46 |
| mm2_exon_Nynrin/Gm26751_chr14_55871430_F | Primer3: not found at this Tm | N.d. | 0.45 |
| mm2_exon_Nynrin/Gm26751_chr14_55871430_R | Primer3: not found at this Tm | N.d. | 0.45 |
| mm4_intergenic_Dact2|Smoc2_chr17_14222133_F | Primer3: not found at this Tm | N.d. | 0.40 |
| mm4_intergenic_Dact2|Smoc2_chr17_14222133_R | Primer3: not found at this Tm | N.d. | 0.40 |
| mm2_intergenic_Kctd3|Kcnk2_chr1_189088957_F | Primer3: not found at this Tm | N.d. | 0.40 |
| mm2_intergenic_Kctd3|Kcnk2_chr1_189088957_R | Primer3: not found at this Tm | N.d. | 0.40 |
| mm4_intron_Tmc7_chr7_118582401_F | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4_intron_Tmc7_chr7_118582401_R | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4_exon_Krba1_chr6_48416008_F | Primer3: not found at this Tm | N.d. | 0.38 |
| mm4_exon_Krba1_chr6_48416008_R | Primer3: not found at this Tm | N.d. | 0.38 |
| mm4_intergenic_Olfr1219|Olfr1220_chr2_89093622_F | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4_intergenic_Olfr1219|Olfr1220_chr2_89093622_R | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4_exon_Wsb2_chr5_117365248_F | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_exon_Wsb2_chr5_117365248_R | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_exon_Zbtb39/Gpr182_chr10_127742016_F | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_exon_Zbtb39/Gpr182_chr10_127742016_R | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_intron_Zfp64_chr2_168911038_F | TCGTCGGCAGCGTCGGGGCAAGTAGCTGAAGATGA | 59.7 | 0.30 |
| mm4_intron_Zfp64_chr2_168911038_R | GTCTCGTGGGCTCGGGTACCTTAGGTGGAGTGCCG | 59.8 | 0.30 |
| mm4_intergenic_Rora|F830001A07Rik_chr9_68823588_F | TCGTCGGCAGCGTCTATCGTGGTGGAGCTGATGC | 59.8 | 0.29 |
| mm4_intergenic_Rora|F830001A07Rik_chr9_68823588_R | GTCTCGTGGGCTCGGATGACTGACCATGCCTGACC | 59.7 | 0.29 |
| mm4_intergenic_Gm23829|Gm5974_chr1_48166818_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_Gm23829|Gm5974_chr1_48166818_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm3_intron_Fads2_chr19_10100900_F | TCGTCGGCAGCGTCGGCCATCTTCCACTTGGGG | 60.3 | 0.26 |
| mm3_intron_Fads2_chr19_10100900_R | GTCTCGTGGGCTCGGTGCATGTTCACAGCGCATAC | 59.5 | 0.26 |
| mm4_intergenic_Zfp335|Gm11458/Zfp335_chr2_164911238_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_Zfp335|Gm11458/Zfp335_chr2_164911238_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_exon_Myl2_chr5_122100987_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_exon_Myl2_chr5_122100987_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Gm15416|Tenm4_chr7_96823488_F | TCGTCGGCAGCGTCACCAGCGCTTTAGCTTAGCA | 60.0 | 0.25 |
| mm4_intergenic_Gm15416|Tenm4_chr7_96823488_R | GTCTCGTGGGCTCGGGCATATGGTGTGCACGTTCT | 58.9 | 0.25 |
| mm4_exon_Olfr353_chr2_36890047_F | TCGTCGGCAGCGTCTCGCAGCTGCTACAACATCC | 60.7 | 0.25 |
| mm4_exon_Olfr353_chr2_36890047_R | GTCTCGTGGGCTCGGTTCTGGCGGGAGTTCAAAGG | 60.2 | 0.25 |
| mm3_intergenic_Synj1|Synj1/4930404I05Rik_chr16_91010663_F | TCGTCGGCAGCGTCCCGGAGAGGGACGGGATTAT | 60.5 | 0.25 |
| mm3_intergenic_Synj1|Synj1/4930404I05Rik_chr16_91010663_R | GTCTCGTGGGCTCGGGGATCCATTCGTCACGGTGA | 59.8 | 0.25 |
| mm4_intergenic_Nsun4|Uqcrh_chr4_116056483_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Nsun4|Uqcrh_chr4_116056483_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Gm25577|Gm22537_chr12_118542880_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Gm25577|Gm22537_chr12_118542880_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Tex2_chr11_106565956_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Tex2_chr11_106565956_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Kat8_chr7_127913916_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Kat8_chr7_127913916_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_exon_Rrm2b_chr15_37954536_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_exon_Rrm2b_chr15_37954536_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Kcnh8|Efhb_chr17_53042716_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Kcnh8|Efhb_chr17_53042716_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Dgat2_chr7_99176681_F | TCGTCGGCAGCGTCAGGACCAAGCTCAGAGGGAT | 59.9 | 0.23 |
| mm4_intron_Dgat2_chr7_99176681_R | GTCTCGTGGGCTCGGCAGGGCCCATGACTTCCTTA | 59.0 | 0.23 |
| mm4_intergenic_Barhl1|Gm13394_chr2_28924064_F | TCGTCGGCAGCGTCAAGATGCAGGGCCAGGAATC | 60.1 | 0.23 |
| mm4_intergenic_Barhl1|Gm13394_chr2_28924064_R | GTCTCGTGGGCTCGGGCATCGCCAGATCTCCATGA | 59.9 | 0.23 |
| mm4_exon_Tanc1_chr2_59630194_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_exon_Tanc1_chr2_59630194_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Trhr2|Gm20681_chr8_122363266_F | TCGTCGGCAGCGTCGTCCCAGGGCCAATCTGTAC | 60.1 | 0.22 |
| mm4_intergenic_Trhr2|Gm20681_chr8_122363266_R | GTCTCGTGGGCTCGGCAAGCCCAAGAGGACAGTGT | 59.8 | 0.22 |
| mm4_intron_Rnf40_chr7_127591871_F | TCGTCGGCAGCGTCGCTTTCAGGGAGGCCAGATT | 60.0 | 0.22 |
| mm4_intron_Rnf40_chr7_127591871_R | GTCTCGTGGGCTCGGATGCCAGGAAACGGACTCTG | 60.0 | 0.22 |
| mm4_intron_Myzap/Gcom1_chr9_71550964_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Myzap/Gcom1_chr9_71550964_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Heatr5b_chr17_78788474_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Heatr5b_chr17_78788474_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Ddx42_chr11_106234163_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Ddx42_chr11_106234163_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Zc3h12a|1700041M05Rik_chr4_125142024_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Zc3h12a|1700041M05Rik_chr4_125142024_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Cmip_chr8_117417548_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Cmip_chr8_117417548_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Fam65a_chr8_105620730_F | TCGTCGGCAGCGTCATCCGTGTCCTCCAGAGTGT | 60.2 | 0.21 |
| mm4_intron_Fam65a_chr8_105620730_R | GTCTCGTGGGCTCGGCTGTCTAGCTCATGCCCAGG | 59.8 | 0.21 |
| mm4_intergenic_Gm23624|4930593C16Rik_chr9_120923004_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Gm23624|4930593C16Rik_chr9_120923004_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Mir29b-1/Gm27019|Gm13834_chr6_31068370_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Mir29b-1/Gm27019|Gm13834_chr6_31068370_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Gm5507|Redrum_chr18_54202224_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Gm5507|Redrum_chr18_54202224_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_exon_Tdgf1_chr9_110945997_F | TCGTCGGCAGCGTCAGCCATTCTCGAAACACCCA | 59.6 | 0.18 |
| mm4_exon_Tdgf1_chr9_110945997_R | GTCTCGTGGGCTCGGTACACGGAGATCTTGGCTGC | 59.8 | 0.18 |
| mm4_intergenic_Cacna1a|Gm16183/Cacna1a_chr8_84602134_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Cacna1a|Gm16183/Cacna1a_chr8_84602134_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_exon_Gad1-ps_chr10_99446189_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_exon_Gad1-ps_chr10_99446189_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_exon_Nfrkb_chr9_31386253_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_exon_Nfrkb_chr9_31386253_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Pgap1|Ankrd44_chr1_54615490_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Pgap1|Ankrd44_chr1_54615490_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_B3galnt1|Gm25621_chr3_69681681_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_B3galnt1|Gm25621_chr3_69681681_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Manbal|Src_chr2_157396800_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Manbal|Src_chr2_157396800_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Ermp1|Mlana_chr19_29670120_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Ermp1|Mlana_chr19_29670120_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Atp1a4_chr1_172243965_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Atp1a4_chr1_172243965_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_exon_Gm17619_chr2_170192549_F | TCGTCGGCAGCGTCAGGGGCAATTAACAGGTAAGCT | 59.6 | 0.15 |
| mm4_exon_Gm17619_chr2_170192549_R | GTCTCGTGGGCTCGGGTCCCTGGTTTGCTGTTTGC | 60.2 | 0.15 |
| mm4_intergenic_Gm24038|Utp14a_chrX_48244404_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm24038|Utp14a_chrX_48244404_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Lin52_chr12_84508821_F | TCGTCGGCAGCGTCTGTTGGGATGCTGCGTGTAT | 60.0 | 0.14 |
| mm4_intron_Lin52_chr12_84508821_R | GTCTCGTGGGCTCGGAAGCATAGAGGCCAGCACTG | 60.1 | 0.14 |
| mm4_intergenic_Gm5529|Gm23934_chr1_80038907_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm5529|Gm23934_chr1_80038907_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Tars|Npr3_chr15_11637578_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Tars|Npr3_chr15_11637578_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Rad51ap2|Gm23781_chr12_11649905_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Rad51ap2|Gm23781_chr12_11649905_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Mir446q|Tnik_chr3_28474332_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Mir446q|Tnik_chr3_28474332_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm7488|Tbl1xr1_chr3_21460757_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm7488|Tbl1xr1_chr3_21460757_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Nbeal2|Ccdc12_chr9_110654210_F | TCGTCGGCAGCGTCACACTTGGTTGTCTGTGGCA | 60.0 | 0.13 |
| mm4_intergenic_Nbeal2|Ccdc12_chr9_110654210_R | GTCTCGTGGGCTCGGTCCTCCTCCAGCCTGACTTC | 60.6 | 0.13 |
| mm4_intron_Zfp385b_chr2_77440604_F | TCGTCGGCAGCGTCAATGCCATATACCAAGAAAGTTTCA | 57.2 | 0.13 |
| mm4_intron_Zfp385b_chr2_77440604_R | GTCTCGTGGGCTCGGAGAAGTCATGGTGCTCTAAGGA | 58.5 | 0.13 |
| mm4_intron_Gli2_chr1_119003472_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Gli2_chr1_119003472_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Dytn|Gm12748_chr1_63666868_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Dytn|Gm12748_chr1_63666868_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_exon_Asmt_chrX_170676326_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_exon_Asmt_chrX_170676326_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm22183|mmu-mir-378d_chr10_126354871_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm22183|mmu-mir-378d_chr10_126354871_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Isg20|Gm26633_chr7_78986619_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Isg20|Gm26633_chr7_78986619_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm25693|Gm12129_chr11_38059074_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm25693|Gm12129_chr11_38059074_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Isg20|Gm26633_chr7_78930718_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Isg20|Gm26633_chr7_78930718_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Hoga1_chr19_42065488_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Hoga1_chr19_42065488_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_4933416M07Rik_chr8_27145705_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_4933416M07Rik_chr8_27145705_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Il9r|Snrnp25_chr11_32201160_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Il9r|Snrnp25_chr11_32201160_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Ppip5k1_chr2_121320979_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Ppip5k1_chr2_121320979_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Plekha7_chr7_116288629_F | TCGTCGGCAGCGTCGGGATGACTACCTGGGGACT | 60.0 | 0.09 |
| mm4_intron_Plekha7_chr7_116288629_R | GTCTCGTGGGCTCGGGCCAGCAACTTCCTCTCACT | 59.9 | 0.09 |
| mm3_intergenic_Gm11375|Gm11376_chr13_31378059_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm3_intergenic_Gm11375|Gm11376_chr13_31378059_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Hal_chr10_93509723_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Hal_chr10_93509723_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Epha5_chr5_84068672_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Epha5_chr5_84068672_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Acaa2|Gm24559_chr18_74901974_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Acaa2|Gm24559_chr18_74901974_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intergenic_Gm25433|Senp6_chr9_80055959_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intergenic_Gm25433|Senp6_chr9_80055959_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_A930011O12Rik/Mir124a-1/Mir3078|Kif13b_chr14_64607820_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_A930011O12Rik/Mir124a-1/Mir3078|Kif13b_chr14_64607820_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm27043|Gm25020_chr13_17071228_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm27043|Gm25020_chr13_17071228_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_n-R5s157|Gm25423_chr7_116675079_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_n-R5s157|Gm25423_chr7_116675079_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_mmu-mir-6336|Lrp1b_chr2_42579662_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_mmu-mir-6336|Lrp1b_chr2_42579662_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Ryr1_chr7_29078196_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Ryr1_chr7_29078196_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intron_Col13a1_chr10_61963367_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intron_Col13a1_chr10_61963367_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intergenic_Scaf4|Hunk_chr16_90292830_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intergenic_Scaf4|Hunk_chr16_90292830_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_4930474N05Rik|Gm24394_chr14_36460837_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_4930474N05Rik|Gm24394_chr14_36460837_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Peli2|Gm23881_chr14_48361056_F | TCGTCGGCAGCGTCTCTTTCCACGGTGTGCTGTT | 60.1 | 0.06 |
| mm4_intergenic_Peli2|Gm23881_chr14_48361056_R | GTCTCGTGGGCTCGGCATTTCTCGAACCCCAGGCA | 60.3 | 0.06 |
| mm3_intron_Fam163b_chr2_27118673_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intron_Fam163b_chr2_27118673_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Specc1l_chr10_75245868_F | TCGTCGGCAGCGTCGTGTACCAGCCCCTCACTTC | 60.0 | 0.05 |
| mm4_exon_Specc1l_chr10_75245868_R | GTCTCGTGGGCTCGGCATTCCCACTGCTCCCCTTC | 60.3 | 0.05 |
| mm4_intron_Phf17_chr3_41557558_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Phf17_chr3_41557558_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Itgav_chr2_83777781_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Itgav_chr2_83777781_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Diap3|Gm23278_chr14_87345276_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Diap3|Gm23278_chr14_87345276_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Tex29|A230072I06Rik_chr8_12208176_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Tex29|A230072I06Rik_chr8_12208176_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Aldh1a3|Asb7_chr7_66626677_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Aldh1a3|Asb7_chr7_66626677_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Rftn2_chr1_55201884_F | TCGTCGGCAGCGTCCACAGACTCGGACACTGGTC | 60.0 | 0.05 |
| mm4_exon_Rftn2_chr1_55201884_R | GTCTCGTGGGCTCGGGCCAAACCTCTACTCACCCC | 60.0 | 0.05 |
| mm4_exon_Syt13_chr2_92954521_F | TCGTCGGCAGCGTCGGGATTGGAGTGGGTTGTGT | 59.8 | 0.05 |
| mm4_exon_Syt13_chr2_92954521_R | GTCTCGTGGGCTCGGGGTGACATGACAGCTGTCCT | 59.6 | 0.05 |
| mm4_intergenic_Gm9848|Gzmk_chr13_113156795_F | TCGTCGGCAGCGTCCAAGCACTCTGAGGATGGGG | 60.1 | 0.04 |
| mm4_intergenic_Gm9848|Gzmk_chr13_113156795_R | GTCTCGTGGGCTCGGCTTCCACAGACAGCTCCCAG | 60.0 | 0.04 |
| mm4_intron_Sntg1_chr1_9220951_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Sntg1_chr1_9220951_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Uty_chrY_1201504_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Uty_chrY_1201504_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Mgam_chr6_40634046_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Mgam_chr6_40634046_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Mcmbp_chr7_128699954_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Mcmbp_chr7_128699954_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm26099|Gm24809_chrX_37067034_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm26099|Gm24809_chrX_37067034_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm26076|Gm25519_chr3_111465444_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm26076|Gm25519_chr3_111465444_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm24345|Gm22401_chr14_100625709_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm24345|Gm22401_chr14_100625709_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Zc3h12c_chr9_52167944_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Zc3h12c_chr9_52167944_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Ndufaf5_chr2_140184137_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Ndufaf5_chr2_140184137_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Ntm|Gm26435_chr9_30133793_F | TCGTCGGCAGCGTCATCAAGTTGCTACAGCTGCT | 57.5 | 0.04 |
| mm4_intergenic_Ntm|Gm26435_chr9_30133793_R | GTCTCGTGGGCTCGGCACGGGCACAGGTAGGTAAA | 59.6 | 0.04 |
| mm4_intron_Fam160b1_chr19_57376554_F | TCGTCGGCAGCGTCGGGAAGCAGTGAGTTGAGCT | 59.9 | 0.04 |
| mm4_intron_Fam160b1_chr19_57376554_R | GTCTCGTGGGCTCGGCGCTGGCATCACTTCTCTGT | 60.3 | 0.04 |
| mm3_intergenic_Gm24280|Rbm26/Gm17066_chr14_105074328_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_Gm24280|Rbm26/Gm17066_chr14_105074328_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Nsun2|Ube2ql1_chr13_69695771_F | TCGTCGGCAGCGTCCTGTCTCTAAGCCTGTGTGCT | 59.7 | 0.04 |
| mm4_intergenic_Nsun2|Ube2ql1_chr13_69695771_R | GTCTCGTGGGCTCGGCTGCCTCAGACCCGTCTTTT | 59.9 | 0.04 |
| mm4_intergenic_Gm11523|Cdk5rap3_chr11_96901091_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm11523|Cdk5rap3_chr11_96901091_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm2_exon_Nfatc1_chr18_80698420_F | TCGTCGGCAGCGTCGTACGTCTTCCACCTCCACG | 60.1 | 0.03 |
| mm2_exon_Nfatc1_chr18_80698420_R | GTCTCGTGGGCTCGGTTCCTGTCCTCTGGCAACAC | 59.8 | 0.03 |
| mm4_intergenic_Gm24297|Gm22911_chr10_38440913_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm24297|Gm22911_chr10_38440913_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Atp6v1b2|Lzts1_chr8_69115921_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Atp6v1b2|Lzts1_chr8_69115921_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Arhgef33_chr17_80358448_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Arhgef33_chr17_80358448_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Stk35|Tgm3_chr2_129842980_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Stk35|Tgm3_chr2_129842980_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Cntn5|Gm25365_chr9_11936144_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Cntn5|Gm25365_chr9_11936144_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_5730522E02Rik_chr11_26065799_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_5730522E02Rik_chr11_26065799_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Eif4ebp2|D830039M14Rik/Lrrc20_chr10_61462080_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Eif4ebp2|D830039M14Rik/Lrrc20_chr10_61462080_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Dct|Gm26773_chr14_118093342_F | TCGTCGGCAGCGTCATTAATGGCGCAGTGGACCA | 60.0 | 0.03 |
| mm4_intergenic_Dct|Gm26773_chr14_118093342_R | GTCTCGTGGGCTCGGCTGCCCTGCCCCTCTTTT | 59.5 | 0.03 |
| mm4_exon_Creld1_chr6_113492672_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Creld1_chr6_113492672_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm12439|Gm12438_chr4_48720101_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm12439|Gm12438_chr4_48720101_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm12057|RP23-242C19.7/B3gnt2_chr11_22833558_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm12057|RP23-242C19.7/B3gnt2_chr11_22833558_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Zfp385b_chr2_77443079_F | TCGTCGGCAGCGTCCCAAAACTGGCCTCACTGGA | 60.1 | 0.02 |
| mm4_intron_Zfp385b_chr2_77443079_R | GTCTCGTGGGCTCGGAAGTGCCCAGGTGTAGTTGG | 59.8 | 0.02 |
| mm3_exon_Tmem233_chr5_116040633_F | TCGTCGGCAGCGTCTCCCGACTAGTGGGTGTTCA | 60.1 | 0.02 |
| mm3_exon_Tmem233_chr5_116040633_R | GTCTCGTGGGCTCGGCTCATCTGCACACACTCCCA | 59.6 | 0.02 |
| mm4_intron_Blvrb_chr7_27449210_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Blvrb_chr7_27449210_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm17399|Gm22198_chr9_118239873_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm17399|Gm22198_chr9_118239873_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Pds5a|1700022K14Rik_chr5_65745261_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Pds5a|1700022K14Rik_chr5_65745261_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Tle3|Rplp1_chr9_61783371_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Tle3|Rplp1_chr9_61783371_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gprc5d|Hebp1_chr6_135119503_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gprc5d|Hebp1_chr6_135119503_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm6311|Gm23319_chr13_75959706_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm6311|Gm23319_chr13_75959706_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Igf1r_chr7_68160316_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Igf1r_chr7_68160316_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Nlgn2|Tmem256_chr11_69837855_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Nlgn2|Tmem256_chr11_69837855_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Rtn4rl1_chr11_75228016_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Rtn4rl1_chr11_75228016_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Lama3_chr18_12359153_F | TCGTCGGCAGCGTCCCACATCACCTTGGAGCTGT | 59.9 | 0.00 |
| mm4_intron_Lama3_chr18_12359153_R | GTCTCGTGGGCTCGGAGCTGCCACAAGAAAGTCCA | 59.8 | 0.00 |
| mm4_intergenic_Tsen54|Llgl2_chr11_115823914_F | TCGTCGGCAGCGTCCACTACTGGGGCCACGATTG | 60.7 | 0.00 |
| mm4_intergenic_Tsen54|Llgl2_chr11_115823914_R | GTCTCGTGGGCTCGGTTACATACAGGGCGAGCCTG | 59.5 | 0.00 |
| mm4_intergenic_Slco6c1|D1Ertd622e_chr1_97366524_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Slco6c1|D1Ertd622e_chr1_97366524_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Mep1a|Ankrd66_chr17_43519025_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Mep1a|Ankrd66_chr17_43519025_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_1700013D24Rik|Pex5_chr6_124392546_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_1700013D24Rik|Pex5_chr6_124392546_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Lamb3_chr1_193302013_F | TCGTCGGCAGCGTCAGTGACATCAGATGGGCGTG | 60.1 | 0.00 |
| mm4_exon_Lamb3_chr1_193302013_R | GTCTCGTGGGCTCGGAATGTGGGGTCCCCTCTCTT | 60.1 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| mm4_exon_Fam181b/Gm9934_chr7_93081075 |
TGACCTTGAACGAGCCTGTGCGCCTCCTGTACCCCACAGCCTTGGACTCTCCAGGTGGGGAGGACGCGCCAGCCTTGTCT TCTTTCACCCCCTTCTTCCCAGATTGTGCGTTGCCGCCGCCCCATCAGGTGTCCTATG |
| mm3_intron_Sufu_chr19_46477778 |
AGTTGCTGTGCTAGGAGCTGCTTAATCAAAGGCCTGTTATGGGCTCATTAGTGTGGTCCACCACGGGCCACCCCTCAGGA GAGTCCTGGGAAGGCTGGCTTAGGTCCCGCCCTTCAGGCACGCACCCTTGATCACCAAG |
| mm4_intron_Btbd16_chr7_130797862 |
TGCTTCTGGCTTTGACCCTTAGGTGTGTAATCTAGCCGCACCATCTCATGGTCTGACACAGCCCCACAGGGACCTTCCTC GGACTCTCCACAGAGGTGTAGCCACACTGTGCATGTGTCCTCCT |
| mm4_intron_Zfp64_chr2_168911038 |
GGGGCAAGTAGCTGAAGATGACGGAGGAATGTGTAGACATATAAAGGTATCCCCCTTCCCCGGGCTCTTCAGAAGTGGGG GATCTGCCAGTAGGAAGGCGGCACTCCACCTAAGGTAC |
| mm4_intergenic_Rora|F830001A07Rik_chr9_68823588 |
TATCGTGGTGGAGCTGATGCTTGAAAAACACATGTTTACCCAAAGTCTGGTGAGTTCCATGCCTCTGGAGAGTCCTGGGT TTTGTTTCCTTGCGGGTCAGGCATGGTCAGTCAT |
| mm3_intron_Fads2_chr19_10100900 |
GGCCATCTTCCACTTGGGGAGCGCCGAACACGAAGGCTGGAGCGCCCCCTTTGGAGAGTCCCAGGCTCCGGCTGGAACAG CGCGCAGTATGCGCTGTGAACATGCA |
| mm4_intergenic_Gm15416|Tenm4_chr7_96823488 |
ACCAGCGCTTTAGCTTAGCATCTCTGGTGTTCATACTTTCTCTCTCCTCCGAAATCTCCTATAAACTCTTCCCACTGACT CTTCAGAGGAGGGAAGAACGTGCACACCATATGC |
| mm4_exon_Olfr353_chr2_36890047 |
TCGCAGCTGCTACAACATCCTTCTCCGTGGTCTCTCCAGAGGAAGGGAACAGATAGACACTGAAGAGTGTTCCATAGAAA ACACAGACCACACAGAGATGGGAACTACAAGTAGAAAAGGCCTTTGAACTCCCGCCAGAA |
| mm3_intergenic_Synj1|Synj1/4930404I05Rik_chr16_91010663 |
CCGGAGAGGGACGGGATTATCACACCGCGGACTCTCCAGGGCGGCGGCGAAGGGCAGGGAAAGCCACACAGGTGACTGCC GCCTCCTGCCCAGTCACGAAGCTCACCGTGACGAATGGATCC |
| mm4_intron_Dgat2_chr7_99176681 |
AGGACCAAGCTCAGAGGGATGTCCCCGGACTGTCCTGGGGAGGGGATACAACAGCTCAGATGAGGTATCAGCCTAACATA AGGAAGTCATGGGCCCTG |
| mm4_intergenic_Barhl1|Gm13394_chr2_28924064 |
AAGATGCAGGGCCAGGAATCTCCATGGTCTGCCTCTGGCTCTCCAGAGGGTGGCGCTGTAGGACAAGGAGCTGAGGGTCT ACACTGAGATCATGGAGATCTGGCGATGC |
| mm4_intergenic_Trhr2|Gm20681_chr8_122363266 |
GTCCCAGGGCCAATCTGTACCAACATCAGCAGCCTAGGTCTCTCCAGCAGGGGGGCGGGGAGGGGAAGCATGGACACTGT CCTCTTGGGCTTG |
| mm4_intron_Rnf40_chr7_127591871 |
GCTTTCAGGGAGGCCAGATTACACTCAGCATGCAGAAGGTAAGCAGCCTCCTCCCCCTCCCCTCCAGGTCTGCCTCGGAC TTTCCTGAGGGCAGGCATCTTCATCAGAGTCCGTTTCCTGGCAT |
| mm4_intron_Fam65a_chr8_105620730 |
ATCCGTGTCCTCCAGAGTGTGGCCAAGGAGTCCCTGGGCATGAGCTAGACCGGGGACCCTCCAGAGGGTGGCCAAGGAGT CCCTGGGCATGAGCTAGACAG |
| mm4_exon_Tdgf1_chr9_110945997 |
AGCCATTCTCGAAACACCCAAAGGCTAATTTCACAAAATGAAAACAGTAGATTAAAAAGAAAGATATGCCAAATGAGAGA GGCCCCCGTCCTGGAGAGTCTGTGGGAAGTTAGCAGCCAAGATCTCCGTGTA |
| mm4_exon_Gm17619_chr2_170192549 |
AGGGGCAATTAACAGGTAAGCTGTTGTATAAGTACATGCTTCAAATAACCTCAGGCTCTCCAGAAGTGGGGACAGCAAAC AGCAAACCAGGGAC |
| mm4_intron_Lin52_chr12_84508821 |
TGTTGGGATGCTGCGTGTATTCAGCAGTCACGGTGCAAGTCTGATCAAGGCATGTATTCCCAAACCGCTCGGCCTCTGTC TCTCCAGGGGAGGGGCCAGTGCTGGCCTCTATGCTT |
| mm4_intergenic_Nbeal2|Ccdc12_chr9_110654210 |
ACACTTGGTTGTCTGTGGCAGCGGCCACCGCCCCGCCTCTCCTAGGACTGTCCAAAGGGCGGGGAAGTCAGGCTGGAGGA GGA |
| mm4_intron_Zfp385b_chr2_77440604 |
AATGCCATATACCAAGAAAGTTTCATAAATAGCTGGAAATTAAATCTATGAGATGACAAAATTCTTAGTGATGGACACAA ATGCCACCTGGGACTTTCCAGAACGGGGGCATGTTTTTCCTTAGAGCACCATGACTTCT |
| mm4_intron_Plekha7_chr7_116288629 |
GGGATGACTACCTGGGGACTCAGTCCACCCCTCTGCTGAGTCCTGGGAGGAGGGACGGGCAGGAGGTAGTCCTCAGTAGT GAGAGGAAGTTGCTGGC |
| mm4_intergenic_Peli2|Gm23881_chr14_48361056 |
TCTTTCCACGGTGTGCTGTTCACATGGGACTCTGTGTGGAAGACAATGGCCCATTCTTTTCTCTCAAGTTTAGCTTTCCA GGAGGCCCCCTCTCTGGAGAGCGCTGGGTCCTTGTGGTGCCTGGGGTTCGAGAAATG |
| mm4_exon_Specc1l_chr10_75245868 |
GTGTACCAGCCCCTCACTTCCAGCGATGATGCTCTGGACGCCCCCTCCTCTTCAGAGTCAGAGGGCGTGCCAAGCATTGA GCGCTCTCGGAAGGGGAGCAGTGGGAATG |
| mm4_exon_Rftn2_chr1_55201884 |
CACAGACTCGGACACTGGTCAGCCTCCCCTCTGCAGTGTCCTCGGAAAGTCCTAGTGACACACACTCCAGTGTCCAGACC GCAGGCTGAGGGGGGTGAGTAGAGGTTTGGC |
| mm4_exon_Syt13_chr2_92954521 |
GGGATTGGAGTGGGTTGTGTTTGTTTTTTTTTTATCACACGTAGAGTGCCCGTTCCCTGGCAGGCCTAGGTTGCCACTCC TCTGGAGTGTCTGCGGCATTCTGAGGACAGCTGTCATGTCACC |
| mm4_intergenic_Gm9848|Gzmk_chr13_113156795 |
CAAGCACTCTGAGGATGGGGCCACCGTCTCTCCAGAGGGGGTAGAGAGCAGCCTGTCCCCTGTGGTCTCATAGGATCACA TGGGGAGACATGACTGGTAACTCAGTCCTCTGTGTCTGGGAGCTGTCTGTGGAAG |
| mm4_intergenic_Ntm|Gm26435_chr9_30133793 |
ATCAAGTTGCTACAGCTGCTAATCACTATGAGGTAATTCCATCCCCCAGACTACCCCACCTTTGGAGAGTGCTAGGCAAT TGAGGGAAGGAGGGGGCCTCAGTTCATGAGTTTACCTACCTGTGCCCGTG |
| mm4_intron_Fam160b1_chr19_57376554 |
GGGAAGCAGTGAGTTGAGCTGAGTGGGCCCCCAGTCTGCAGTGTCCGTGGCAGGCTCAGAGTGCTGCAGGATGCTCATGT GTGGATACTGGAAAGGCCCTTGGAATGTGGGATACAGAGAAGTGATGCCAGCG |
| mm4_intergenic_Nsun2|Ube2ql1_chr13_69695771 |
CTGTCTCTAAGCCTGTGTGCTTCTGTGCCCACAGCTCCCCCTCTGTGGTGTCCGTGGAGGTGAGAAAAAGACGGGTCTGA GGCAG |
| mm2_exon_Nfatc1_chr18_80698420 |
GTACGTCTTCCACCTCCACGTCGTGGAAAAACTGGCCGCTGCCATGGTGTAGGCCCAGGTAGGAGGTGATCTCGATTCTC GGACTCTCCAGAGTCGGGGCCCCGTTGGGTCTGGTGTTGCCAGAGGACAGGAA |
| mm4_intergenic_Dct|Gm26773_chr14_118093342 |
ATTAATGGCGCAGTGGACCAGCCTTCCAGGTGTTTTCTTCTCCCCATCTGTGTGCCTCTGCCTCCTACTCTCCAGAGGTA GGGATAAAGCCTAATTATTTCCTAAATCCTTGTTAATAAAAAAAGAGGGGCAGGGCAG |
| mm4_intron_Zfp385b_chr2_77443079 |
CCAAAACTGGCCTCACTGGACACTGCCAAGGCTGACTCCTGGCACTCTCCAGATGGGAGGATCCAACTACACCTGGGCAC TT |
| mm3_exon_Tmem233_chr5_116040633 |
TCCCGACTAGTGGGTGTTCATTTGCCAGGGACCCGCCTCTGGAGAGTCCTGGAGGAAAAGATCTTACAGTTTTAAAATCA AGGATCCATTCCTCAGAAACTTCTTTGGGAGTGTGTGCAGATGAG |
| mm4_intron_Lama3_chr18_12359153 |
CCACATCACCTTGGAGCTGTGTCTATTCAAATCTCCTTCCATGTTAGCCCCGGAGTTTCCTGAGGGCGGGACTGTTTCTT CCTGCTCCTGGACTTTCTTGTGGCAGCT |
| mm4_intergenic_Tsen54|Llgl2_chr11_115823914 |
CACTACTGGGGCCACGATTGGTTGGAGAGCGGCCCCGCTCTGGAGACTCCCAGGATGCGCTCCTGCCCCGTCGGCTCGGC CTAGGAGGCGGGGCCGGGGCGGGGCGAGGGCTCGGCAGGCTCGCCCTGTATGTAA |
| mm4_exon_Lamb3_chr1_193302013 |
AGTGACATCAGATGGGCGTGGCAGCTGCCAGATTCCTGAGGCCGCCAGCAGTGGGGCTACACCCAGCCAGGGAGTCTCCA GAGGTGAGGCTGTTGTTTAAAACTGTGGAGCCAAGAGAGGGGACCCCACATT |