CRISPOR

DNA break repair outcome predictions

Guide: TGGCACTCTGGTTGGTAAGA GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TTTCTGGATGGGGGTCAGCTTGGCACTCTGGTTGGTAAGAGGGTCTCTAATTTGTCCGAAGCCGTGAGCAGCCCGCTGCC`	(Wild-type sequence)
335	`GGGGTCAGCTTGGCACTCTGGTTGGT--------CTCTAATTTGTCCGAAGCCGTGAGCA`	`8 bp deleted → frameshift`
282	`GGGGTCAGCTTGGCACTCTGGTTGG-------GTCTCTAATTTGTCCGAAGCCGTGAGCA`	`7 bp deleted → frameshift`
274	`GGGGTCAGCTTGGCACTCTGGT------------CTCTAATTTGTCCGAAGCCGTGAGCA`	`12 bp deleted → no frameshift`
232	`GGGGTCAGCTTGGCACTCT-------------------AATTTGTCCGAAGCCGTGAGCA`	`19 bp deleted → frameshift`
230	`GGGGTCAGCTTGGCACTCTGG-----------GTCTCTAATTTGTCCGAAGCCGTGAGCA`	`11 bp deleted → frameshift`
211	`GGGGTC-----------------------------TCTAATTTGTCCGAAGCCGTGAGCA`	`29 bp deleted → frameshift`
170	`GGGGTCAGCTTGGCACTCT-----------------CTAATTTGTCCGAAGCCGTGAGCA`	`17 bp deleted → frameshift`
164	`GGGGTCAGCTTGGCACTCTGGTTGGTAA------------TTTGTCCGAAGCCGTGAGCA`	`12 bp deleted → no frameshift`
154	`GGGGTCAGCTTGG-------------------GTCTCTAATTTGTCCGAAGCCGTGAGCA`	`19 bp deleted → frameshift`
147	`GGGGTCAGCTTGGCACTCTGGTTG--------------------TCCGAAGCCGTGAGCA`	`20 bp deleted → frameshift`
147	`GGGGTCAGCTTGG--------------------TCTCTAATTTGTCCGAAGCCGTGAGCA`	`20 bp deleted → frameshift`
133	`GGG------------------------------TCTCTAATTTGTCCGAAGCCGTGAGCA`	`30 bp deleted → no frameshift`
133	`GGGGTCAGCTTGGCACTCTGGTTGGTAAG----------------------CCGTGAGCA`	`22 bp deleted → frameshift`
116	`GGGGTCAGCTTGGCACTCTGGTTGGTAAGA-------------------AGCCGTGAGCA`	`19 bp deleted → frameshift`
116	`GGGGTCAGCTTGGCACTCTGGTTGGT-------------------CCGAAGCCGTGAGCA`	`19 bp deleted → frameshift`
105	`GGGGTCAGCTTGGCACT---------------------AATTTGTCCGAAGCCGTGAGCA`	`21 bp deleted → no frameshift`
98	`GGGG----------------------------GTCTCTAATTTGTCCGAAGCCGTGAGCA`	`28 bp deleted → frameshift`
95	`GGGGTCAGCTTGGCACTCTGGT-----------------------CCGAAGCCGTGAGCA`	`23 bp deleted → frameshift`
90	`GGGGTCAGCTTGGCACTCTG------------------------TCCGAAGCCGTGAGCA`	`24 bp deleted → no frameshift`
84	`GG-------------------------------TCTCTAATTTGTCCGAAGCCGTGAGCA`	`31 bp deleted → frameshift`
81	`GGGGTCAGCT--------------------------CTAATTTGTCCGAAGCCGTGAGCA`	`26 bp deleted → frameshift`
80	`GGGGTCAGCTTG--------------------------------TCCGAAGCCGTGAGCA`	`32 bp deleted → frameshift`
77	`GGGGTCAGCTTGGCACTCTGGTTGGTAAGA---------------------------GCA`	`27 bp deleted → no frameshift`
77	`GGGGTCAGCTTGGCACTCTGGTT-------------------TGTCCGAAGCCGTGAGCA`	`19 bp deleted → frameshift`
74	`GGGGTCAGCTTGGCACTC----------------------------CGAAGCCGTGAGCA`	`28 bp deleted → frameshift`
74	`GGGGTCAGCT----------------------------AATTTGTCCGAAGCCGTGAGCA`	`28 bp deleted → frameshift`
70	`GGGGTCAGCTTGGCACTCTGGTTGGTAAG-----------------------------CA`	`29 bp deleted → frameshift`
70	`GGGGTCAGCTTGGCACTCTGGTTGGT-----------------------------GAGCA`	`29 bp deleted → frameshift`
67	`GGGGTC----------------------------------------CGAAGCCGTGAGCA`	`40 bp deleted → frameshift`
63	`GGGGTC-------------------------------TAATTTGTCCGAAGCCGTGAGCA`	`31 bp deleted → frameshift`
60	`GGGGTCAGCTTGGCACTCTGGTTG--------------------------------AGCA`	`32 bp deleted → frameshift`
60	`GGGGTCAGCTTGGC--------------------------------------CGTGAGCA`	`38 bp deleted → frameshift`
58	`GGGGTCAGC-------------------------------------------CGTGAGCA`	`43 bp deleted → frameshift`
57	`GGGGTCAGCTTGGCACTCTGGT---------------------------------GAGCA`	`33 bp deleted → no frameshift`
52	`GGGGTCAGCTTGGCA---------------------------------------------`	`45 bp deleted → no frameshift`
49	`GGGGTCAGCTTGGCACTCTG------------------------------------AGCA`	`36 bp deleted → no frameshift`
42	`GGGGTCAGCTT-------------------------------TGTCCGAAGCCGTGAGCA`	`31 bp deleted → frameshift`
41	`GGGGTCAGC--------------------------------------------------A`	`50 bp deleted → frameshift`
33	`GGGGTCAGCTTG--------------------------------------------AGCA`	`44 bp deleted → frameshift`
24	`GGGGT--------------------------------------------------GAGCA`	`50 bp deleted → frameshift`
21	`GGGGTCA-----------------------------------------------------`	`53 bp deleted → frameshift`

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