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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314129_F | TCGTCGGCAGCGTCGTCGATGGACTAGCAGGCAG | 60.2 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314129_R | GTCTCGTGGGCTCGGAGCTACTAGGTTGCCCTTGG | 59.0 | 1.00 |
| mm3_exon_Mospd3_chr5_137599736_F | Primer3: not found at this Tm | N.d. | 0.56 |
| mm3_exon_Mospd3_chr5_137599736_R | Primer3: not found at this Tm | N.d. | 0.56 |
| mm4_intron_9530059O14Rik_chr9_122659252_F | TCGTCGGCAGCGTCACCAGGGCTAGCCTTCTCTA | 59.3 | 0.56 |
| mm4_intron_9530059O14Rik_chr9_122659252_R | GTCTCGTGGGCTCGGTGGGCCAACCATGTGTTATG | 58.4 | 0.56 |
| mm4_intron_Slc52a3_chr2_152007413_F | TCGTCGGCAGCGTCCTAACCTTGGAAGCCCAGGC | 60.6 | 0.54 |
| mm4_intron_Slc52a3_chr2_152007413_R | GTCTCGTGGGCTCGGGCGACCTGTGGAGAAGTGAA | 59.9 | 0.54 |
| mm4_intron_Gpr64_chrX_160442553_F | Primer3: not found at this Tm | N.d. | 0.48 |
| mm4_intron_Gpr64_chrX_160442553_R | Primer3: not found at this Tm | N.d. | 0.48 |
| mm4_intergenic_Irs2|Irs2/9530052E02Rik_chr8_10997641_F | TCGTCGGCAGCGTCGAGTAACAGTGGGGTGTGGG | 59.9 | 0.46 |
| mm4_intergenic_Irs2|Irs2/9530052E02Rik_chr8_10997641_R | GTCTCGTGGGCTCGGTCATCTGAGTGCTGTGCCTG | 60.0 | 0.46 |
| mm4_intergenic_Tgm3|Gm22424_chr2_130057449_F | TCGTCGGCAGCGTCACTTGTGCTGTTCTGAGTTGTG | 59.3 | 0.42 |
| mm4_intergenic_Tgm3|Gm22424_chr2_130057449_R | GTCTCGTGGGCTCGGTGTGTGCTGAAGATGGAGGT | 58.9 | 0.42 |
| mm4_intergenic_Adra1a|Gm10032_chr14_66761904_F | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4_intergenic_Adra1a|Gm10032_chr14_66761904_R | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4_intron_Parm1_chr5_91618938_F | TCGTCGGCAGCGTCTTCCTTCCTGCTCAGCTGTG | 59.9 | 0.34 |
| mm4_intron_Parm1_chr5_91618938_R | GTCTCGTGGGCTCGGTGTTTGTCCACGGGTACAGG | 59.8 | 0.34 |
| mm4_intergenic_Gm25549|Mapkapk2_chr1_131083065_F | TCGTCGGCAGCGTCTGACTGGTTTCTAGCTGCCC | 59.6 | 0.33 |
| mm4_intergenic_Gm25549|Mapkapk2_chr1_131083065_R | GTCTCGTGGGCTCGGTTATGCAGCACAGGACAGGG | 60.0 | 0.33 |
| mm4_intergenic_1700017N19Rik|Gm26278_chr10_100632527_F | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_intergenic_1700017N19Rik|Gm26278_chr10_100632527_R | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_intergenic_Tmem179|Inf2_chr12_112569094_F | TCGTCGGCAGCGTCAGTCCTTGCCTGCACATACC | 60.0 | 0.32 |
| mm4_intergenic_Tmem179|Inf2_chr12_112569094_R | GTCTCGTGGGCTCGGAGGTCTCCAGTACACTGTGGA | 59.8 | 0.32 |
| mm4_intron_Abcg1_chr17_31069226_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intron_Abcg1_chr17_31069226_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Camkmt|Rpl31-ps16_chr17_85483123_F | TCGTCGGCAGCGTCATCACCAAAGCCCAGGTTGT | 59.8 | 0.26 |
| mm4_intergenic_Camkmt|Rpl31-ps16_chr17_85483123_R | GTCTCGTGGGCTCGGCCGTGTGTTTATCCCAAGGC | 59.1 | 0.26 |
| mm4_intron_Sart3_chr5_113747339_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intron_Sart3_chr5_113747339_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_Rps12-ps6|Astx1a_chrX_124396077_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_Rps12-ps6|Astx1a_chrX_124396077_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_Rps12-ps18|Astx1b_chrX_124899692_F | TCGTCGGCAGCGTCACCTGCAGAAGCCACAGTAG | 59.6 | 0.26 |
| mm4_intergenic_Rps12-ps18|Astx1b_chrX_124899692_R | GTCTCGTGGGCTCGGTAGACCGCAGATGTCCTGGA | 60.0 | 0.26 |
| mm4_intergenic_Rps12-ps17|Astx4c_chrX_125594117_F | TCGTCGGCAGCGTCACCTGCAGAAGCCACAGTAG | 59.6 | 0.26 |
| mm4_intergenic_Rps12-ps17|Astx4c_chrX_125594117_R | GTCTCGTGGGCTCGGTAGACCGCAGATGTCCTGGA | 60.0 | 0.26 |
| mm4_intergenic_Gm17361|Gm21616_chrX_125331079_F | TCGTCGGCAGCGTCACCTGCAGAAGCCACAGTAG | 59.6 | 0.26 |
| mm4_intergenic_Gm17361|Gm21616_chrX_125331079_R | GTCTCGTGGGCTCGGTAGACCGCAGATGTCCTGGA | 60.0 | 0.26 |
| mm4_intron_Drosha_chr15_12870061_F | TCGTCGGCAGCGTCCAGCTGCTCCTTGAGGTCAA | 59.9 | 0.25 |
| mm4_intron_Drosha_chr15_12870061_R | GTCTCGTGGGCTCGGCTGCACTGGGAGAGACAGAC | 59.7 | 0.25 |
| mm4_intergenic_9230114K14Rik|Gm25601_chr5_52327978_F | TCGTCGGCAGCGTCTGGCAGGAAGGGAAGAGAGA | 59.8 | 0.25 |
| mm4_intergenic_9230114K14Rik|Gm25601_chr5_52327978_R | GTCTCGTGGGCTCGGGCTTAGCCAAGGAAGCACCA | 60.6 | 0.25 |
| mm4_intergenic_Celf4|Gm25845_chr18_25590417_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Celf4|Gm25845_chr18_25590417_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Med30|Ext1_chr15_52892796_F | TCGTCGGCAGCGTCTCTGCAGATGGTGTGCTGTT | 59.8 | 0.24 |
| mm4_intergenic_Med30|Ext1_chr15_52892796_R | GTCTCGTGGGCTCGGCCCATAAACCGACAGAGGAGT | 59.1 | 0.24 |
| mm4_intergenic_Tspan9|Gm10010_chr6_128147360_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Tspan9|Gm10010_chr6_128147360_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Rbfox3_chr11_118677299_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Rbfox3_chr11_118677299_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm3_intron_Tbc1d24_chr17_24181694_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm3_intron_Tbc1d24_chr17_24181694_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Gramd1b_chr9_40308027_F | TCGTCGGCAGCGTCTAGAAGGCCTGGACCTCTCC | 60.0 | 0.21 |
| mm4_intron_Gramd1b_chr9_40308027_R | GTCTCGTGGGCTCGGGTCTCAGGGTGTCACCACAG | 59.9 | 0.21 |
| mm4_exon_Smtn/Gm11946/Gm11947_chr11_3535153_F | TCGTCGGCAGCGTCAGAGTTCCTCACCCTCTCGG | 60.3 | 0.20 |
| mm4_exon_Smtn/Gm11946/Gm11947_chr11_3535153_R | GTCTCGTGGGCTCGGTTCGGAAACCATCCCCTTCC | 59.6 | 0.20 |
| mm4_intron_Vstm2l_chr2_157927130_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Vstm2l_chr2_157927130_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Rpl17-ps4|Tnc_chr4_63915996_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Rpl17-ps4|Tnc_chr4_63915996_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_Por_chr5_135732609_F | TCGTCGGCAGCGTCAAGCTGAACCAAGGCACTGA | 59.8 | 0.19 |
| mm4_intron_Por_chr5_135732609_R | GTCTCGTGGGCTCGGAGAGTCAAGAGGAAGCACGC | 60.0 | 0.19 |
| mm4_intron_Npnt_chr3_132949270_F | TCGTCGGCAGCGTCTTTTCTTCCGCGCTCCGAG | 60.7 | 0.18 |
| mm4_intron_Npnt_chr3_132949270_R | GTCTCGTGGGCTCGGGATGCCGCACCTGTTTTACC | 59.8 | 0.18 |
| mm4_intergenic_Lypd2|2300005B03Rik_chr15_74739131_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Lypd2|2300005B03Rik_chr15_74739131_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Dnah2_chr11_69542347_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Dnah2_chr11_69542347_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Mamld1|Gm14730_chrX_71190940_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Mamld1|Gm14730_chrX_71190940_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Arap1_chr7_101406518_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Arap1_chr7_101406518_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm10108|Sema3a_chr5_13260575_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm10108|Sema3a_chr5_13260575_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_exon_Meis3_chr7_16178027_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_exon_Meis3_chr7_16178027_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_4930594M17Rik|Gm26012_chr18_81275483_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_4930594M17Rik|Gm26012_chr18_81275483_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Tiam1|Sod1_chr16_90198835_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Tiam1|Sod1_chr16_90198835_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm13056|Epha2_chr4_141297477_F | TCGTCGGCAGCGTCTCAGGAGGACTCAGAGGCTT | 59.5 | 0.16 |
| mm4_intergenic_Gm13056|Epha2_chr4_141297477_R | GTCTCGTGGGCTCGGCCTGCCTCTGAATCCCACAG | 60.1 | 0.16 |
| mm4_intergenic_Celf2|Gm13389_chr2_6872622_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Celf2|Gm13389_chr2_6872622_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3_exon_Cnp_chr11_100576056_F | TCGTCGGCAGCGTCACCTTACAACCCAGCTGTTGA | 59.5 | 0.16 |
| mm3_exon_Cnp_chr11_100576056_R | GTCTCGTGGGCTCGGGACCGCTTGTCAGTTGAGGA | 59.9 | 0.16 |
| mm3_intron_Tll2_chr19_41095043_F | TCGTCGGCAGCGTCATAACCAAGGCTGCCTGACC | 60.0 | 0.15 |
| mm3_intron_Tll2_chr19_41095043_R | GTCTCGTGGGCTCGGCCAGACTGGCAGGATCCATG | 60.1 | 0.15 |
| mm4_intergenic_Sufu|Trim8_chr19_46501168_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Sufu|Trim8_chr19_46501168_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Camkmt_chr17_85151850_F | TCGTCGGCAGCGTCTGGCAGATCAGGAAGTGCTG | 60.0 | 0.14 |
| mm4_intron_Camkmt_chr17_85151850_R | GTCTCGTGGGCTCGGAATCACTCCCTGACAGCTGC | 60.0 | 0.14 |
| mm4_intron_Pde10a_chr17_8928191_F | TCGTCGGCAGCGTCCTAGTGTGGGATGAGTGGCC | 59.8 | 0.14 |
| mm4_intron_Pde10a_chr17_8928191_R | GTCTCGTGGGCTCGGTCTTTGTTTCTGGGCCACCC | 60.4 | 0.14 |
| mm4_intron_Map2k5_chr9_63302914_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Map2k5_chr9_63302914_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_4930412M03Rik|Rai1_chr11_60140050_F | TCGTCGGCAGCGTCCATCGTGCTAGCCCAGTGG | 60.5 | 0.14 |
| mm4_intergenic_4930412M03Rik|Rai1_chr11_60140050_R | GTCTCGTGGGCTCGGGAGTGCCCTCAGTAGCCATT | 59.4 | 0.14 |
| mm4_intron_Cldn10_chr14_118868540_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Cldn10_chr14_118868540_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_exon_Zfp276_chr8_123257173_F | TCGTCGGCAGCGTCTGGTGCTCATCTGGCATTCA | 59.6 | 0.14 |
| mm4_exon_Zfp276_chr8_123257173_R | GTCTCGTGGGCTCGGGCCTCTTTGTCTGCCTCCAT | 60.0 | 0.14 |
| mm4_intron_Shroom2_chrX_152705541_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Shroom2_chrX_152705541_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Amer2|Spata13_chr14_60597416_F | TCGTCGGCAGCGTCCTGAGTGGGAGGCCAAGAAG | 60.0 | 0.13 |
| mm4_intergenic_Amer2|Spata13_chr14_60597416_R | GTCTCGTGGGCTCGGAAGAAGGTCTGTGGGTGCAG | 59.8 | 0.13 |
| mm4_intergenic_Id3|E2f2_chr4_136160114_F | TCGTCGGCAGCGTCCTGCCACCTCCAAATGGGAC | 60.9 | 0.13 |
| mm4_intergenic_Id3|E2f2_chr4_136160114_R | GTCTCGTGGGCTCGGGCTGACCCAGAAGGCTTGAA | 60.2 | 0.13 |
| mm4_intron_Nfxl1_chr5_72526187_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Nfxl1_chr5_72526187_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_exon_Glis1_chr4_107627574_F | TCGTCGGCAGCGTCCACAAAACGGGCTTGCTTCA | 59.8 | 0.12 |
| mm4_exon_Glis1_chr4_107627574_R | GTCTCGTGGGCTCGGATACCCATCCCTGGTGCTCT | 60.0 | 0.12 |
| mm4_intron_Gm15686_chr6_144748208_F | TCGTCGGCAGCGTCTCATTGCATGGAGACCGGTG | 60.3 | 0.10 |
| mm4_intron_Gm15686_chr6_144748208_R | GTCTCGTGGGCTCGGCCACACTCACAGAGTTGGCT | 59.8 | 0.10 |
| mm4_intergenic_Gm11426|Cct6b_chr11_82641380_F | TCGTCGGCAGCGTCTAGGACATTCAGCAGCCACC | 59.7 | 0.10 |
| mm4_intergenic_Gm11426|Cct6b_chr11_82641380_R | GTCTCGTGGGCTCGGGCCCTGCTCCCTAACTTCTG | 60.1 | 0.10 |
| mm4_intergenic_Gm11419|Gm17268_chr11_82006976_F | TCGTCGGCAGCGTCATCTGGCTGGAGAAAACGGG | 60.0 | 0.09 |
| mm4_intergenic_Gm11419|Gm17268_chr11_82006976_R | GTCTCGTGGGCTCGGTGTGTGACGAAGCTGGGAAG | 60.2 | 0.09 |
| mm4_intergenic_Gm22070|Tph2_chr10_115023728_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm22070|Tph2_chr10_115023728_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_9330160F10Rik/2310047M10Rik_chr11_69059152_F | TCGTCGGCAGCGTCTGACAACAAACAAACAAACAACCC | 59.4 | 0.09 |
| mm4_exon_9330160F10Rik/2310047M10Rik_chr11_69059152_R | GTCTCGTGGGCTCGGGTCGTGGACTTTCCTGTGCT | 60.2 | 0.09 |
| mm3_intergenic_Snrnp48|Bmp6_chr13_38264449_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm3_intergenic_Snrnp48|Bmp6_chr13_38264449_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm3_intergenic_Rabl2|Syt10_chr15_89725520_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm3_intergenic_Rabl2|Syt10_chr15_89725520_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm3_intergenic_Ptgs2|Gm26687_chr1_150145768_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm3_intergenic_Ptgs2|Gm26687_chr1_150145768_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm3_intergenic_1700082M22Rik|Ces2a_chr8_104673714_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm3_intergenic_1700082M22Rik|Ces2a_chr8_104673714_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Araf_chrX_20857932_F | TCGTCGGCAGCGTCCTACCTCCACGCCAAGAACA | 59.6 | 0.09 |
| mm4_intron_Araf_chrX_20857932_R | GTCTCGTGGGCTCGGTCACCTCCAGAATCTTACCCC | 58.5 | 0.09 |
| mm4_intergenic_Gm12469|Gm25687_chr4_54314007_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm12469|Gm25687_chr4_54314007_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Pard3_chr8_127118354_F | TCGTCGGCAGCGTCTCTTGCCCTAACATAGGCGC | 60.1 | 0.09 |
| mm4_intron_Pard3_chr8_127118354_R | GTCTCGTGGGCTCGGGAGGACCTCACAACCTGCAC | 60.6 | 0.09 |
| mm4_intergenic_4933402D24Rik|Gm13751_chr1_63815452_F | TCGTCGGCAGCGTCAGCAATAGCCAGTGACCCAC | 60.0 | 0.09 |
| mm4_intergenic_4933402D24Rik|Gm13751_chr1_63815452_R | GTCTCGTGGGCTCGGGGCTCAGGCTCTGCTATCTC | 59.6 | 0.09 |
| mm4_intergenic_Mir28b|Large_chr8_73029051_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Mir28b|Large_chr8_73029051_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm3_intergenic_Ppp1r37|Gemin7_chr7_19563865_F | TCGTCGGCAGCGTCTAACCACCAACCTGCAGCAT | 59.8 | 0.08 |
| mm3_intergenic_Ppp1r37|Gemin7_chr7_19563865_R | GTCTCGTGGGCTCGGCCCTCTTGGTGATGTGGTCC | 60.0 | 0.08 |
| mm4_intron_Trerf1_chr17_47174836_F | TCGTCGGCAGCGTCACTTCTTCCTGGCAGGCATC | 60.0 | 0.08 |
| mm4_intron_Trerf1_chr17_47174836_R | GTCTCGTGGGCTCGGAAGAAGTTGCAGCCCATCGA | 59.9 | 0.08 |
| mm3_intergenic_Wdr61|Crabp1_chr9_54740726_F | TCGTCGGCAGCGTCGCTGCAGGACTGAAGGGAAT | 60.0 | 0.07 |
| mm3_intergenic_Wdr61|Crabp1_chr9_54740726_R | GTCTCGTGGGCTCGGACAAGAGACTGGCTGCTGTG | 60.2 | 0.07 |
| mm4_intron_Scgb1c1_chr7_140846525_F | TCGTCGGCAGCGTCGGTCACCATGCTCAGGAGTC | 60.1 | 0.07 |
| mm4_intron_Scgb1c1_chr7_140846525_R | GTCTCGTGGGCTCGGCCTGGTAACCAACAACCGGA | 59.8 | 0.07 |
| mm4_intron_Prkg2_chr5_98954114_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Prkg2_chr5_98954114_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Abcc8_chr7_46154366_F | TCGTCGGCAGCGTCGGTTTTGTGTCCTACTGCCT | 57.7 | 0.07 |
| mm4_intron_Abcc8_chr7_46154366_R | GTCTCGTGGGCTCGGGGCCATCGACACAAATCAGC | 59.9 | 0.07 |
| mm4_intron_Vps4a_chr8_107031538_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Vps4a_chr8_107031538_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_exon_Snapc4/Gm13562_chr2_26365764_F | TCGTCGGCAGCGTCGTTGCAGGGCCTACTACTGG | 60.1 | 0.07 |
| mm4_exon_Snapc4/Gm13562_chr2_26365764_R | GTCTCGTGGGCTCGGCCACTCAGGCTCTTGGTCTC | 59.7 | 0.07 |
| mm4_intergenic_Gm9806|Foxb2_chr19_16804812_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm9806|Foxb2_chr19_16804812_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Vldlr|Kcnv2_chr19_27256225_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Vldlr|Kcnv2_chr19_27256225_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Gm711_chr2_26939641_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Gm711_chr2_26939641_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Camk2n1|Vwa5b1_chr4_138490102_F | TCGTCGGCAGCGTCTGACTTATTTATTCACCCACAGCC | 59.0 | 0.06 |
| mm4_intergenic_Camk2n1|Vwa5b1_chr4_138490102_R | GTCTCGTGGGCTCGGCCTGGAGGGATGAAGACCGA | 60.6 | 0.06 |
| mm4_exon_Jund/Gm11175_chr8_70698960_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Jund/Gm11175_chr8_70698960_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Ywhae|Gm26836_chr11_75735175_F | TCGTCGGCAGCGTCAGTGAGTTGGGCTCCTGTTG | 59.8 | 0.05 |
| mm4_intergenic_Ywhae|Gm26836_chr11_75735175_R | GTCTCGTGGGCTCGGCTTCTGGGATCGAGAGGGGA | 60.1 | 0.05 |
| mm4_intron_Gm13026_chr4_140458762_F | TCGTCGGCAGCGTCGCTGACTTTGGCATCTGCAC | 60.1 | 0.05 |
| mm4_intron_Gm13026_chr4_140458762_R | GTCTCGTGGGCTCGGCAAAAGCTGAGCTGGTGCAG | 60.0 | 0.05 |
| mm4_exon_Pcdh20_chr14_88468014_F | TCGTCGGCAGCGTCCCACTGATTCTCTGGGTGGC | 60.3 | 0.05 |
| mm4_exon_Pcdh20_chr14_88468014_R | GTCTCGTGGGCTCGGCAGTGTCACAGGGATTCTGACA | 59.9 | 0.05 |
| mm4_intergenic_Fastkd3|1700001L19Rik_chr13_68594430_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Fastkd3|1700001L19Rik_chr13_68594430_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Tec_chr5_72764854_F | TCGTCGGCAGCGTCAGCTAGTTTACCTCCCACCC | 58.4 | 0.05 |
| mm4_intron_Tec_chr5_72764854_R | GTCTCGTGGGCTCGGACTCCTACCTCTCTGTGCCT | 59.2 | 0.05 |
| mm4_intergenic_Gm11371|Gm11372_chr13_31061957_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm11371|Gm11372_chr13_31061957_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Grm4_chr17_27512471_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Grm4_chr17_27512471_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm10051|Gm15627_chr5_134087672_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm10051|Gm15627_chr5_134087672_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Wnt6_chr1_74772102_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Wnt6_chr1_74772102_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Snx9_chr17_5904307_F | TCGTCGGCAGCGTCGTGTAGGCTCATCTGAACCTGT | 59.7 | 0.03 |
| mm4_intron_Snx9_chr17_5904307_R | GTCTCGTGGGCTCGGACATAAGGCTTTAATGTCTTCCCT | 57.9 | 0.03 |
| mm4_intron_Phf21b_chr15_84849524_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Phf21b_chr15_84849524_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Vps8_chr16_21453330_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Vps8_chr16_21453330_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_mmu-mir-21c|Nrp1/Mir1903_chr8_128358961_F | TCGTCGGCAGCGTCATCTCCAGCCAGGCAATCAC | 60.1 | 0.02 |
| mm4_intergenic_mmu-mir-21c|Nrp1/Mir1903_chr8_128358961_R | GTCTCGTGGGCTCGGAAGATGAGCGGGAGAGCAAG | 59.8 | 0.02 |
| mm4_intron_Atp8a2_chr14_60005796_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Atp8a2_chr14_60005796_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Rpl35a|Lmln_chr16_33062480_F | TCGTCGGCAGCGTCGCGTAATGCCGGCCAATG | 59.9 | 0.02 |
| mm4_intergenic_Rpl35a|Lmln_chr16_33062480_R | GTCTCGTGGGCTCGGTCGTTACCATGGAGTGCGAC | 60.1 | 0.02 |
| mm4_intron_Glra1_chr11_55534531_F | TCGTCGGCAGCGTCGAGCCCTTCTGTGACATCCC | 60.1 | 0.02 |
| mm4_intron_Glra1_chr11_55534531_R | GTCTCGTGGGCTCGGACCTGTTGGAGAGAGAGCCT | 59.8 | 0.02 |
| mm3_intron_Col2a1_chr15_98003913_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intron_Col2a1_chr15_98003913_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Sh2d5/Kif17|Sh2d5_chr4_138250576_F | TCGTCGGCAGCGTCGGTGAGTGCTCCTGGGAAC | 60.0 | 0.01 |
| mm4_intergenic_Sh2d5/Kif17|Sh2d5_chr4_138250576_R | GTCTCGTGGGCTCGGCGCCAGAAAGGGAGGTATGG | 60.1 | 0.01 |
| mm4_intergenic_4930571K23Rik|Kdm8_chr7_125409873_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_4930571K23Rik|Kdm8_chr7_125409873_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Kif3c_chr12_3393046_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Kif3c_chr12_3393046_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Casz1_chr4_148804927_F | TCGTCGGCAGCGTCACTTTGCAACTAGGGTGGGG | 59.8 | 0.01 |
| mm4_intron_Casz1_chr4_148804927_R | GTCTCGTGGGCTCGGGCCTCCTTCTCTCCTCCAGA | 60.0 | 0.01 |
| mm4_intergenic_Kdelr2|Daglb_chr5_143458213_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Kdelr2|Daglb_chr5_143458213_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Onecut2_chr18_64342751_F | TCGTCGGCAGCGTCTGTAAGTCCCGCCTTTGCAT | 59.9 | 0.01 |
| mm4_intron_Onecut2_chr18_64342751_R | GTCTCGTGGGCTCGGGGAACAATGTAACCAGCGCG | 60.1 | 0.01 |
| mm4_intron_Zc3h7b_chr15_81746166_F | TCGTCGGCAGCGTCACTGGGATTGTGGCGATTGA | 59.6 | 0.00 |
| mm4_intron_Zc3h7b_chr15_81746166_R | GTCTCGTGGGCTCGGATGGATCGCAGAAGGGACCT | 60.6 | 0.00 |
| mm4_intron_Ano1_chr7_144655553_F | TCGTCGGCAGCGTCTGGGTTCCACAGTTACACGG | 59.8 | 0.00 |
| mm4_intron_Ano1_chr7_144655553_R | GTCTCGTGGGCTCGGGGGAGAAGCAACACCTGTGA | 59.8 | 0.00 |
| mm4_intron_Msi2_chr11_88613960_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Msi2_chr11_88613960_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Nsmce4a_chr7_130566932_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Nsmce4a_chr7_130566932_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm26674/Sash1|Gm9930_chr10_8932973_F | TCGTCGGCAGCGTCAACAAGGCTGGGTGTTCAAA | 58.1 | 0.00 |
| mm4_intergenic_Gm26674/Sash1|Gm9930_chr10_8932973_R | GTCTCGTGGGCTCGGAGAGCCACCTCTCTCCACTG | 60.6 | 0.00 |
| mm4_intron_Snx9_chr17_5903596_F | TCGTCGGCAGCGTCCTTGGAGACTCACTGCGTGT | 59.9 | 0.00 |
| mm4_intron_Snx9_chr17_5903596_R | GTCTCGTGGGCTCGGACTGAGAGTTTCGGATCCGC | 59.8 | 0.00 |
| mm4_intron_Mef2b/2310045N01Rik_chr8_70157176_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Mef2b/2310045N01Rik_chr8_70157176_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Ccdc85c_chr12_108241488_F | TCGTCGGCAGCGTCATCTCTCTGTGCCAGCAACC | 60.0 | 0.00 |
| mm4_intron_Ccdc85c_chr12_108241488_R | GTCTCGTGGGCTCGGCTTAGCCCACCTGCTTGGAG | 60.3 | 0.00 |
| mm4_intron_Adamts10_chr17_33525458_F | TCGTCGGCAGCGTCCTAGTGGCCTCCCTGCAG | 59.0 | 0.00 |
| mm4_intron_Adamts10_chr17_33525458_R | GTCTCGTGGGCTCGGTCTGTCTGTCTCTGTCTCTCC | 57.9 | 0.00 |
| mm4_intergenic_Tfap2c|Gm22773_chr2_172860958_F | TCGTCGGCAGCGTCGCTGGCATTAGGGGTGAAGT | 60.0 | 0.00 |
| mm4_intergenic_Tfap2c|Gm22773_chr2_172860958_R | GTCTCGTGGGCTCGGTCAAACCTGTCCAGTGAGCC | 59.8 | 0.00 |
| mm4_intergenic_Gm16045|Sept9_chr11_117266134_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm16045|Sept9_chr11_117266134_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm14005|Gm14010_chr2_128395578_F | TCGTCGGCAGCGTCTGTGTGCTCTCAGAGGCAAG | 59.9 | 0.00 |
| mm4_intergenic_Gm14005|Gm14010_chr2_128395578_R | GTCTCGTGGGCTCGGTTCCCAGGCCCTCTACCTTT | 60.1 | 0.00 |
| mm4_exon_Soga2_chr17_66344146_F | TCGTCGGCAGCGTCATACCAGGAGGGATCCGGAC | 60.1 | 0.00 |
| mm4_exon_Soga2_chr17_66344146_R | GTCTCGTGGGCTCGGAGCAACGACTGGGATTACGC | 60.7 | 0.00 |
| mm3_intergenic_Gm14168|Gm14169_chr2_156585162_F | TCGTCGGCAGCGTCGCCCAGTGGAGCTTCCCC | 62.4 | 0.00 |
| mm3_intergenic_Gm14168|Gm14169_chr2_156585162_R | GTCTCGTGGGCTCGGGCTAAATAGGATCACAATGCGGG | 59.8 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314129 |
GTCGATGGACTAGCAGGCAGTGCTGCAGGGCTGGGCGCCGAGCCACGGTCTGCTGGAGCGGCCATGCTTGGCCCGGGACC CCCAGTCCCCTCCGCGGACAGCCTCTCTGGCCAAGGGCAACCTAGTAGCT |
| mm4_intron_9530059O14Rik_chr9_122659252 |
ACCAGGGCTAGCCTTCTCTACAGTCTGAGGTAGCCCCACCAGTTCCCTCCGTGCACAAAGCTTGGTATTGCCTGTATCAC TTTCCATGTCGTAAGAACTAGTTCCCATAACACATGGTTGGCCCA |
| mm4_intron_Slc52a3_chr2_152007413 |
CTAACCTTGGAAGCCCAGGCTACTCCCTGTCCATGAAGGGGACAGGAGGCAGGCTTCTCAACCCCTTCTATGCCCTTCTT CACTTCTCCACAGGTCGC |
| mm4_intergenic_Irs2|Irs2/9530052E02Rik_chr8_10997641 |
GAGTAACAGTGGGGTGTGGGGTGTGGGTGGGGTCTGGTGGGGGGTGGCTGTGGAGGGGACTGGTGGGTCCTGCTGCACAT GTTCAGTTGTTCCAGCGGAGAGAGCTGACCCCAGGATACAGGCACAGCACTCAGATGA |
| mm4_intergenic_Tgm3|Gm22424_chr2_130057449 |
ACTTGTGCTGTTCTGAGTTGTGATAAAAATGGAAATTTAATACAACAACCCATCAGTCCCCTCCTCAGATAGTCCATCTT TTCTGATTTGTGCTGAATAAACCTCCATCTTCAGCACACA |
| mm4_intron_Parm1_chr5_91618938 |
TTCCTTCCTGCTCAGCTGTGATAAGCACTAATGATTGAAAAGTGAGGTCTCCTCTGGCAGAGGAGAGGACTGGGGGTGAA TTCATCAACCTAGGTGGAGGTTTGCCAAGAGCTGGCCTGTACCCGTGGACAAACA |
| mm4_intergenic_Gm25549|Mapkapk2_chr1_131083065 |
TGACTGGTTTCTAGCTGCCCTTCCCCAAGTCCCCTCCATGTACAGATGAGTCCGGATAAAGAATGACTGTAACCAGAAAA TGTTCTGTGCGGACAGAATATCCTCTCCCCTGTCCTGTGCTGCATAA |
| mm4_intergenic_Tmem179|Inf2_chr12_112569094 |
AGTCCTTGCCTGCACATACCCTGTATCCCTGCCCCCAGTTCCTTCCCAGGACAGAAGAATATTATCCACAGTGTACTGGA GACCT |
| mm4_intergenic_Camkmt|Rpl31-ps16_chr17_85483123 |
ATCACCAAAGCCCAGGTTGTCTGTGTGCAGAGGAGACTGGAGGCCTGTTGAATGAGTAACAATTATGTATCCATTGGACC ATTTTTGAGTAGAAGGTGATCCAAGCTCTAGTTTCCCTTTGCCTTGGGATAAACACACGG |
| mm4_intergenic_Rps12-ps18|Astx1b_chrX_124899692 |
ACCTGCAGAAGCCACAGTAGGAAAGTCCCTGTCTGCTGAGGAGACTGTGGGAACTTTGCTTCCCACTCCAGGACATCTGC GGTCTA |
| mm4_intergenic_Rps12-ps17|Astx4c_chrX_125594117 |
ACCTGCAGAAGCCACAGTAGGAAAGTCCCTGTCTGCTGAGGAGACTGTGGGAACTTTGCTTCCCACTCCAGGACATCTGC GGTCTA |
| mm4_intergenic_Gm17361|Gm21616_chrX_125331079 |
ACCTGCAGAAGCCACAGTAGGAAAGTCCCTGTCTGCTGAGGAGACTGTGGGAACTTTGCTTCCCACTCCAGGACATCTGC GGTCTA |
| mm4_intron_Drosha_chr15_12870061 |
CAGCTGCTCCTTGAGGTCAAGAATAAAGACTGCACTCTCCTTCACCCTCCTGTCCCTCCTGTCCCCTCTTCTGACAGAGT CTCCACCTTGACTCTTGATGTCTGTCTCTCCCAGTGCAG |
| mm4_intergenic_9230114K14Rik|Gm25601_chr5_52327978 |
TGGCAGGAAGGGAAGAGAGAGGGCTGTTTGCAGAGGGGACAGGGGGCACAGTGGGGAGGCAGAAGGGAAAGATTGCAGGT AGCTGCTCTATTCCAGACTTGTGCTGACAGAGCCCCACCATGGTGCTTCCTTGGCTAAGC |
| mm4_intergenic_Med30|Ext1_chr15_52892796 |
TCTGCAGATGGTGTGCTGTTCGGTGCCAGCATTATTTATCCACAAGTCCCCTTCTCGCACAGCTCCCTCCTTGAGCTAAT GTGGAGAATATTAAACATTGAACAATTATCTAACTCCTCTGTCGGTTTATGGG |
| mm4_intron_Gramd1b_chr9_40308027 |
TAGAAGGCCTGGACCTCTCCTGTACAGAAGAGAGGATGAAGCAGACAGTGACCGAGGAGGAGACTGGAGGCTGTGGTGAC ACCCTGAGAC |
| mm4_exon_Smtn/Gm11946/Gm11947_chr11_3535153 |
AGAGTTCCTCACCCTCTCGGTCACCCCCACTGTGTGTGGAGGGGACTGCGGGAGGGAGGGAAGGAAATACAAAGAACTAT TGTCCGCCAGGGGGAAGGGGATGGTTTCCGAA |
| mm4_intron_Por_chr5_135732609 |
AAGCTGAACCAAGGCACTGAGAGGCATCTAATGCACCTGGAATTGGACATCTCAGACTCCAAGATCAGGTGCCTGCTTCC TCCAGTCTCCCCTGCGGCCAGCCTGGCCCCAAGTCCTCTGCGTGCTTCCTCTTGACTCT |
| mm4_intron_Npnt_chr3_132949270 |
TTTTCTTCCGCGCTCCGAGGCTTTTCCTCTGGTCCCCTCCAGGGACAGGAAAGCCAATCAATCCTGGAATTGATTCGTTC CAAGATTGCAGGTAAAACAGGTGCGGCATC |
| mm4_intergenic_Gm13056|Epha2_chr4_141297477 |
TCAGGAGGACTCAGAGGCTTAGGAAACGACCTGTCCCCGGAAGGCACTGAGGGACAGTCATTTCAGCGACCTGGAAGTTC TGGTTGCAGAGAGCAGATCCGTCATTCCAGGTGGAGGGGCTGTGGGATTCAGAGGCAGG |
| mm3_exon_Cnp_chr11_100576056 |
ACCTTACAACCCAGCTGTTGAAGATACTGAAGGATCTACTGCGAGGAAGAAAATCCACCATTCCCCTCCGGGGAAAGCAT CCACCATTCAAGGGTGCTTTAAGTTAGGCTTGGGGATTCCTCAACTGACAAGCGGTC |
| mm3_intron_Tll2_chr19_41095043 | ATAACCAAGGCTGCCTGACCTGGCCCACCAGTCACCTCCCCAGACAGGCATGGATCCTGCCAGTCTGG |
| mm4_intron_Camkmt_chr17_85151850 |
TGGCAGATCAGGAAGTGCTGAAAGCGATGCCCCTGGCTCTCAGAAGAGGGGACTGGTGGGAGAAGCAATGGACCATTTCC CCTCTTGCAGCTGTCAGGGAGTGATT |
| mm4_intron_Pde10a_chr17_8928191 |
CTAGTGTGGGATGAGTGGCCGATGGAGCCCCCCAGCTCCCTCCACAGACAGGTTTTACGCATGACAGTTGTACCAAAAGG AACAGATCTTTAGAATGAGAGATGTCTGGGTGGCCCAGAAACAAAGA |
| mm4_intergenic_4930412M03Rik|Rai1_chr11_60140050 |
CATCGTGCTAGCCCAGTGGACGTGGCCACAAGGCCGTCTCTAATTACCTCCTGTCCCCCCGGCGGCCAGTGAATAATGGC TACTGAGGGCACTC |
| mm4_exon_Zfp276_chr8_123257173 |
TGGTGCTCATCTGGCATTCAGTCTTCCTCTAAGTCTGTCCTTAGACCACAGGGGACTGGTTGTTCCTGTAGGCTGAGGGG ACTGAGGGAGAGAGAAGAATGGGGTTATTGTCCGCAGGACATGGAGGCAGACAAAGAGGC |
| mm4_intergenic_Amer2|Spata13_chr14_60597416 |
CTGAGTGGGAGGCCAAGAAGGGGACTTCACCCAAACTGCCTGCAGTCCCCTCAAGGGACAGCAGCCTGCAGTCAGCTGGG GAGCTGCACCCACAGACCTTCTT |
| mm4_intergenic_Id3|E2f2_chr4_136160114 |
CTGCCACCTCCAAATGGGACACAGCTGGATCCCCCTCCAGTCCCCTCCCCCAGCAGAACTGATACTGCTTAGGCCTTAAG TTTCAAGCCTTCTGGGTCAGC |
| mm4_exon_Glis1_chr4_107627574 |
CACAAAACGGGCTTGCTTCAGGCATCCTGTCCCCCTCCCACGATGTCCCTTCCAGGCACCACCCACTGGAGGTCCCCACT GGTTCCCACCACCACCTGTCCCCTCTGCCCACAGCTGAGAGCACCAGGGATGGGTAT |
| mm4_intron_Gm15686_chr6_144748208 |
TCATTGCATGGAGACCGGTGGCACCACTGCAGAGACACCACCAGCCCCCTTCTCAGACAGACAAATAGACAATTCCCAGG AAGTTTCTGCATGGATTTTTGTGTCTGTTACTTCCGTTACAGCCAACTCTGTGAGTGTGG |
| mm4_intergenic_Gm11426|Cct6b_chr11_82641380 |
TAGGACATTCAGCAGCCACCATGCTAGGCATGACAGCTTCTATCCTCCTGTCCCCACCAAGGACAGGATTTGGGCAGATC AGAAGTTAGGGAGCAGGGC |
| mm4_intergenic_Gm11419|Gm17268_chr11_82006976 |
ATCTGGCTGGAGAAAACGGGAAACCCCAGCACGAGTGTTGCGACAGCAGATTGCACAATGTCCTGAATCCCTAAGTCCCC CTCCAGTCCCCCCAGAGGAAAGAAGACATTCCTGGGCTGCCTTCCCAGCTTCGTCACACA |
| mm4_exon_9330160F10Rik/2310047M10Rik_chr11_69059152 |
TGACAACAAACAAACAAACAACCCTCAATATTATCCTCCAATGAGAGAAATGCTCTACGCAGAGGGGACAGGAGGCCGAA GAGTGACAAAAGCACAGGAAAGTCCACGAC |
| mm4_intron_Araf_chrX_20857932 |
CTACCTCCACGCCAAGAACATCATTCATCGAGACCTAAAGTCCAACAGTATCTACCTGTCCCTGGCTGGGACTGGGGGTG GGTAGTAATCAGAGTGGTAGTTTAAAGGGATGATTTGGGGGGTAAGATTCTGGAGGTGA |
| mm4_intron_Pard3_chr8_127118354 |
TCTTGCCCTAACATAGGCGCCATTAGAGAACAAAGCTTGCTTGATGTCTTAACTATAAAATATAGCCTTGCTATGTTACA ACTATTTCTAAGGGCCCCCAGTCCCCTGCCCTGAGAGGTGCAGGTTGTGAGGTCCTC |
| mm4_intergenic_4933402D24Rik|Gm13751_chr1_63815452 |
AGCAATAGCCAGTGACCCACATGGAAATGCTATGCCTGTTCTCAGATGGGACTGGAGGGCTTCTTTCCATCTCCCAGCTT TCTTCTGTGAGCTAGAGGTGAGAGGAGAGCATGAGATAGCAGAGCCTGAGCC |
| mm3_intergenic_Ppp1r37|Gemin7_chr7_19563865 |
TAACCACCAACCTGCAGCATTTGCTCCCGGGCACTGGCACGGCACTGGCACACAAGTGTGACCACCACCACCACTCCCAG TCCCCTCCGTGGGAAGACAAACCTGCAGGTGACTGCAGTGGACCACATCACCAAGAGGG |
| mm4_intron_Trerf1_chr17_47174836 |
ACTTCTTCCTGGCAGGCATCCTAGGGACAGCTGGGAGCTGGTGTTCCTCCTCCTGTGTGGGCGGGCAAAGCTCGGGCTGT CCACGCAGGCGATTGGTGGGCGTCGATGGGCTGCAACTTCTT |
| mm3_intergenic_Wdr61|Crabp1_chr9_54740726 |
GCTGCAGGACTGAAGGGAATCCTGGGAGATCTTGCTATTCAGGGACACACACCCCCCAGCCCCCTCCCCGGCCAGCCCAG GAGGCTGTGAGGCACAGCAGCCAGTCTCTTGT |
| mm4_intron_Scgb1c1_chr7_140846525 |
GGTCACCATGCTCAGGAGTCTGTCTCCTCCACTGGTGTGCACAGCACTGTCTGCCGCTGGGACTGGGGGATCCGGTTGTT GGTTACCAGG |
| mm4_intron_Abcc8_chr7_46154366 |
GGTTTTGTGTCCTACTGCCTCCCCCAATGCCCTCCCCTGACAGGAGGTGCCCCCAGCACAAATCTGATACCTGTACTGGC ATAGCCCAGAGGTTTGGGCATAAGAAGAAAAACCACATGAGCTGATTTGTGTCGATGGCC |
| mm4_exon_Snapc4/Gm13562_chr2_26365764 |
GTTGCAGGGCCTACTACTGGGGAACCATGGGGGACTGGCAGCAGAGGGGGCTGGAGGATCACAGGTGAGGAGACCAGCAG CTGGCTGTTGAGACCAAGAGCCTGAGTGG |
| mm4_intergenic_Camk2n1|Vwa5b1_chr4_138490102 |
TGACTTATTTATTCACCCACAGCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNACTCTGAGTACAGGAATTCGGTCTTCATCCCTCCAGG |
| mm4_intergenic_Ywhae|Gm26836_chr11_75735175 |
AGTGAGTTGGGCTCCTGTTGGGAGCTGGAGGAGGTGGGATGGGGAAATAGGAGTGCAGCTCCAGGTGAGCTTGTCTGTCC CGGGAGAGTACTGGAGGTTTCCCCTCTCGATCCCAGAAG |
| mm4_intron_Gm13026_chr4_140458762 |
GCTGACTTTGGCATCTGCACCCCAGGAGACCCCAGGAGATATCATCTCCCTCCAGTCCCCACAGAGCACAGCGAGTCTCC TGCTGGGCTCCTCCTGCACCAGCTCAGCTTTTG |
| mm4_exon_Pcdh20_chr14_88468014 |
CCACTGATTCTCTGGGTGGCGTCCCACAGTCCACTGCTCGGACAGTATACCTGTACTTCTCTTTCTCTTCTCTGTCCAGC TGAGTAGAAACTGTCAGAATCCCTGTGACACTG |
| mm4_intron_Tec_chr5_72764854 | AGCTAGTTTACCTCCCACCCCCCCCAGTCACCTCCCCCCACAGATGAAGGCACAGAGAGGTAGGAGT |
| mm4_intron_Snx9_chr17_5904307 |
GTGTAGGCTCATCTGAACCTGTATCTCCAAGACTTCTGTCTCTTTCGGGGGAGGGGGCTGGGGGAGGGAAGACATTAAAG CCTTATGT |
| mm4_intergenic_mmu-mir-21c|Nrp1/Mir1903_chr8_128358961 |
ATCTCCAGCCAGGCAATCACTGGCAGAGCTGTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNTGCCAGTGGAGAAAGTCCTTGCTCTCCCGCTCATCTT |
| mm4_intergenic_Rpl35a|Lmln_chr16_33062480 |
GCGTAATGCCGGCCAATGGCGAGGCGGCTTACGGCCCGCCACCTGTCCGCGGCGAGGGCGGGCGGCGGCGAGGAGGCGTC GCACTCCATGGTAACGA |
| mm4_intron_Glra1_chr11_55534531 |
GAGCCCTTCTGTGACATCCCAGGCTGGTAGCCCAGGCTGTGAAAACAACCTTGAAAGTCTTTGCTAACACCAGAGCTCTG GCAGCAGAGGGGAGTGGAGGGCTCTTAGACAATCAGGCTCTCTCTCCAACAGGT |
| mm4_intergenic_Sh2d5/Kif17|Sh2d5_chr4_138250576 |
GGTGAGTGCTCCTGGGAACCCTCCGGGGGCTGGGCCGAAGCCGGGAGGGTCTTCCCAGAGCGAGCCGCACCCCCACTTCC CTCCGCCGGCAGTTGCCATCACCTCAGTCCCCGTCACCATACCTCCCTTTCTGGCG |
| mm4_intron_Casz1_chr4_148804927 |
ACTTTGCAACTAGGGTGGGGGCGCTTTCCGCGCAGTGGGCTGGGGGCGCAGGTGCTTGCCAATACTGGGTGATCGTTCCT TCTCCCACGCCCCCTCCTGCTGCAGGGGGGTCGTGAGCTGTCTGGAGGAGAGAAGGAGGC |
| mm4_intron_Onecut2_chr18_64342751 |
TGTAAGTCCCGCCTTTGCATTAAAGCGGGAGCTGATTGTACGCGGAGTGGAGTGGCGGGGAGGCCTGGCAGCCGGGCGGC AGGGGACCGGTCAACGCGCTGGTTACATTGTTCC |
| mm4_intron_Zc3h7b_chr15_81746166 |
ACTGGGATTGTGGCGATTGAGGGGCGCCCTGGCAGGGCTGGGGGCCCTGGGGAAGCAGAGTCGACCCCGCCGCCACTGCC CTCAGCGGACACAAGGTCCCTTCTGCGATCCAT |
| mm4_intron_Ano1_chr7_144655553 |
TGGGTTCCACAGTTACACGGTACTTATAAGAACATGTTCCCCTGTTCGGGGTGGGGAGTGGGGGTCAACCTGGACTTCTC ACTCACAGGTGTTGCTTCTCCC |
| mm4_intergenic_Gm26674/Sash1|Gm9930_chr10_8932973 |
AACAAGGCTGGGTGTTCAAATTCTAGTCCTTAGAACAACCACCACCCCCCTCTGAGGACAGACGTTTAGATGACTAGCTT CCTTTCCAGTGGAGAGAGGTGGCTCT |
| mm4_intron_Snx9_chr17_5903596 |
CTTGGAGACTCACTGCGTGTGGATTTGGACAGTCCGCTGAGGGATCTGGGGGCTGGGGAAAGGGCATAAGGAGTGATGTT AGTAGTTCTCCCAGCAGTCATACTAAAGAAGCGGATCCGAAACTCTCAGT |
| mm4_intron_Ccdc85c_chr12_108241488 |
ATCTCTCTGTGCCAGCAACCCCAGCAGGCTCCTCCGTGGACAGGAGGGGGTGGGGGCTGTGAAGCCACACACTCCAAGCA GGTGGGCTAAG |
| mm4_intron_Adamts10_chr17_33525458 |
CTAGTGGCCTCCCTGCAGCCTGCCAGAGGAGGGGCCTGGGGGACAGGGGCCAAGCCTAGGTNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAGAGACAGAGACAGACAGA |
| mm4_intergenic_Tfap2c|Gm22773_chr2_172860958 |
GCTGGCATTAGGGGTGAAGTGGGGCCAGGGCTGTCCGGGAAGAGGTCTGGTGGGCACATGTGCACAGGGCACTGGGCAGT GAAGCTGGAGAAGAGGCTCCACTGGGGTCTCCCAAAGTGGGGCTCACTGGACAGGTTTGA |
| mm4_intergenic_Gm14005|Gm14010_chr2_128395578 |
TGTGTGCTCTCAGAGGCAAGAGCCTCCAAACCCCTCAGCTGACAGGGGCCAGGACTCGAGAAAGGTAGAGGGCCTGGGAA |
| mm4_exon_Soga2_chr17_66344146 |
ATACCAGGAGGGATCCGGACAAGGCTCTGTGGTGGCTTCATGCCCCACCCTCCCCATCTCGGTCCTGTCTGCCCAGGGGT CTGGAGGCCGATCGGCCTCAGCCCTGGGGGATGCGTAATCCCAGTCGTTGCT |
| mm3_intergenic_Gm14168|Gm14169_chr2_156585162 |
GCCCAGTGGAGCTTCCCCCACTGCCCCCTCCCCCCTCCAGACCCCTCCGTGAACAGCATGTTCCTCCGACTGTTATTTTT AATGCATTTATCCTGATGCTTAATTTATCCCGCATTGTGATCCTATTTAGC |