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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313949_F | TCGTCGGCAGCGTCAATTTGTCCGAAGCCGTGAG | 58.8 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313949_R | GTCTCGTGGGCTCGGCTCCTCCTCCTGGCCTCC | 60.4 | 1.00 |
| mm2_intergenic_Cyp2d12|AC118710.1_chr15_82566128_F | Primer3: not found at this Tm | N.d. | 0.65 |
| mm2_intergenic_Cyp2d12|AC118710.1_chr15_82566128_R | Primer3: not found at this Tm | N.d. | 0.65 |
| mm3_intergenic_Kif13a|Gm24769_chr13_46933911_F | Primer3: not found at this Tm | N.d. | 0.56 |
| mm3_intergenic_Kif13a|Gm24769_chr13_46933911_R | Primer3: not found at this Tm | N.d. | 0.56 |
| mm3_intron_Tnr_chr1_159783643_F | TCGTCGGCAGCGTCTCCTGGCTCACTGATGTCTG | 59.1 | 0.55 |
| mm3_intron_Tnr_chr1_159783643_R | GTCTCGTGGGCTCGGGTGGAGGGTGGCTATTTGCT | 60.0 | 0.55 |
| mm3_intron_Arl15_chr13_114047652_F | TCGTCGGCAGCGTCTCAACCAGGCTTGTGATTGC | 59.0 | 0.50 |
| mm3_intron_Arl15_chr13_114047652_R | GTCTCGTGGGCTCGGGTTAACCTGGACTGGAGCCC | 60.0 | 0.50 |
| mm4_intergenic_Ndst1|Rps14_chr18_60756540_F | TCGTCGGCAGCGTCTGAGAGTCTGGTATCCGGCA | 60.0 | 0.43 |
| mm4_intergenic_Ndst1|Rps14_chr18_60756540_R | GTCTCGTGGGCTCGGCACACGAGAGACATGTGGAGA | 59.4 | 0.43 |
| mm4_intergenic_Gm25650|Gm23584_chr10_47221244_F | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intergenic_Gm25650|Gm23584_chr10_47221244_R | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intergenic_Ispd|Mir5099_chr12_36756575_F | TCGTCGGCAGCGTCACTGAGCAAGTTCCTAGGCA | 58.6 | 0.29 |
| mm4_intergenic_Ispd|Mir5099_chr12_36756575_R | GTCTCGTGGGCTCGGTGTTGTCAACAGGAGCTCACA | 59.7 | 0.29 |
| mm4_intron_Hibadh_chr6_52577439_F | TCGTCGGCAGCGTCACCATTCCCAGTTACCTTGTCA | 59.2 | 0.27 |
| mm4_intron_Hibadh_chr6_52577439_R | GTCTCGTGGGCTCGGATGGGCCACTAGAGGTCACT | 59.9 | 0.27 |
| mm4_intron_D8Ertd82e_chr8_36111939_F | TCGTCGGCAGCGTCCAGTGCCCACTATCAGGAGG | 59.5 | 0.26 |
| mm4_intron_D8Ertd82e_chr8_36111939_R | GTCTCGTGGGCTCGGTCACAGGCTAGGTTCTCCCA | 59.8 | 0.26 |
| mm4_intron_Cdk18_chr1_132130848_F | TCGTCGGCAGCGTCGGTTAGGGCCTGCCTGTAAT | 59.4 | 0.17 |
| mm4_intron_Cdk18_chr1_132130848_R | GTCTCGTGGGCTCGGTGTCAGCCCAGATGGAGAGT | 60.2 | 0.17 |
| mm4_intron_Pebp4_chr14_69989790_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Pebp4_chr14_69989790_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Sptbn1_chr11_30149865_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Sptbn1_chr11_30149865_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_BC021891|Kcnk1_chr8_125959323_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_BC021891|Kcnk1_chr8_125959323_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_exon_Ddx11_chr17_66150010_F | TCGTCGGCAGCGTCGACCCACTACAGGTGTGTGG | 59.9 | 0.12 |
| mm4_exon_Ddx11_chr17_66150010_R | GTCTCGTGGGCTCGGAGCCCTCTGAAACACGACAG | 59.9 | 0.12 |
| mm4_intergenic_Gm25659|Gm25644_chr19_51085314_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm25659|Gm25644_chr19_51085314_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Mrc2_chr11_105328055_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Mrc2_chr11_105328055_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_2310014F06Rik_chr7_112644687_F | TCGTCGGCAGCGTCCCCCTCAGCCCCATTCATC | 59.8 | 0.07 |
| mm4_intron_2310014F06Rik_chr7_112644687_R | GTCTCGTGGGCTCGGCACTCCTCTCTGCTTGGGTG | 60.0 | 0.07 |
| mm4_exon_Irs2/9530052E02Rik_chr8_11006613_F | TCGTCGGCAGCGTCGGGTAAGGGTTGTAGGCCAC | 60.0 | 0.06 |
| mm4_exon_Irs2/9530052E02Rik_chr8_11006613_R | GTCTCGTGGGCTCGGGTACCTCAGCCTTCCTCTGC | 59.8 | 0.06 |
| mm4_intergenic_Gm26976|Cand1_chr10_119071266_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm26976|Cand1_chr10_119071266_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Usp14_chr18_10000555_F | TCGTCGGCAGCGTCACCTACTGCATTTGGTTGCTG | 59.1 | 0.06 |
| mm4_exon_Usp14_chr18_10000555_R | GTCTCGTGGGCTCGGTGGATGTGTATGAACTCTGCACA | 59.9 | 0.06 |
| mm4_intron_Jdp2_chr12_85603516_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Jdp2_chr12_85603516_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Zfp385b_chr2_77695558_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Zfp385b_chr2_77695558_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Ahi1_chr10_20959775_F | TCGTCGGCAGCGTCCACTACCCAGATCGCACAGG | 60.1 | 0.05 |
| mm4_exon_Ahi1_chr10_20959775_R | GTCTCGTGGGCTCGGTGAATCATCTGCTGTCGGGG | 59.8 | 0.05 |
| mm4_intergenic_Gm9578|Gm22891_chr14_82253289_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm9578|Gm22891_chr14_82253289_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Vwa2_chr19_56897900_F | TCGTCGGCAGCGTCGTTCCCTCCCAGCTTCACC | 60.0 | 0.05 |
| mm4_exon_Vwa2_chr19_56897900_R | GTCTCGTGGGCTCGGACAGAGCCATTTCTGCCTCC | 60.0 | 0.05 |
| mm4_intergenic_Gm13481|Gm13489_chr2_48510254_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm13481|Gm13489_chr2_48510254_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm5468|Myo10_chr15_25555208_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm5468|Myo10_chr15_25555208_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Ubr5|Odf1_chr15_38142685_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Ubr5|Odf1_chr15_38142685_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_Slc24a3|BC039771_chr2_145658131_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_Slc24a3|BC039771_chr2_145658131_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Crispld2_chr8_120004636_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Crispld2_chr8_120004636_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm12494|Gstm3_chr3_107953601_F | TCGTCGGCAGCGTCGCCGGTGATGAGATCCAGTC | 60.2 | 0.04 |
| mm4_intergenic_Gm12494|Gstm3_chr3_107953601_R | GTCTCGTGGGCTCGGCTTCCTGAGAACCCAGCAGG | 60.0 | 0.04 |
| mm4_intron_Usp26_chrX_51800161_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Usp26_chrX_51800161_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm25722|Gm10051_chr5_132810711_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm25722|Gm10051_chr5_132810711_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm20619|Gm20617_chr6_6432742_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm20619|Gm20617_chr6_6432742_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Ptprh|Tmem86b_chr7_4624963_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Ptprh|Tmem86b_chr7_4624963_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm24415|Alg10b/mmu-mir-7648_chr15_90126626_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm24415|Alg10b/mmu-mir-7648_chr15_90126626_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_4930593A02Rik_chr3_58703016_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_4930593A02Rik_chr3_58703016_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm9341|Gm24444_chr7_136268900_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm9341|Gm24444_chr7_136268900_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Uvssa_chr5_33385251_F | TCGTCGGCAGCGTCGCCTCCCTCTGTATTGTGCA | 59.7 | 0.02 |
| mm4_intron_Uvssa_chr5_33385251_R | GTCTCGTGGGCTCGGGCTGGTGGTTCAAAGCAGTG | 59.9 | 0.02 |
| mm4_intergenic_Gm26042|Tbx19_chr1_165061688_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm26042|Tbx19_chr1_165061688_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Siva1|Akt1_chr12_112652422_F | TCGTCGGCAGCGTCCATGGCCACAGGGTCTATCC | 59.8 | 0.02 |
| mm4_intergenic_Siva1|Akt1_chr12_112652422_R | GTCTCGTGGGCTCGGTACCCTCTCCCCGAAGACAG | 60.0 | 0.02 |
| mm4_intergenic_mmu-mir-6393|Zdhhc25_chr15_88059803_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_mmu-mir-6393|Zdhhc25_chr15_88059803_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Gm26250|Gm11819_chr4_13403557_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Gm26250|Gm11819_chr4_13403557_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm23605|Kctd8_chr5_69091684_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm23605|Kctd8_chr5_69091684_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Churc1/Fntb|Churc1_chr12_76776883_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Churc1/Fntb|Churc1_chr12_76776883_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm11431|Mrm1_chr11_84792749_F | TCGTCGGCAGCGTCCTCTCCAGCCAGCCTATGTG | 59.8 | 0.01 |
| mm4_intergenic_Gm11431|Mrm1_chr11_84792749_R | GTCTCGTGGGCTCGGCAGGGATTGGGAACGTGGAG | 60.3 | 0.01 |
| mm4_intron_Ttyh2_chr11_114697911_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Ttyh2_chr11_114697911_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Ttn_chr2_76853903_F | TCGTCGGCAGCGTCAGCTGGTGCAGGAACTTTCT | 59.5 | 0.01 |
| mm4_exon_Ttn_chr2_76853903_R | GTCTCGTGGGCTCGGACAAAGAGGGTGTTGTGGCA | 60.0 | 0.01 |
| mm4_intergenic_Gm5091|Dll1_chr17_15292964_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm5091|Dll1_chr17_15292964_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm26585|Slc2a12_chr10_22637839_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm26585|Slc2a12_chr10_22637839_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Ap2s1|Slc1a5_chr7_16767376_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Ap2s1|Slc1a5_chr7_16767376_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Incenp_chr19_9898621_F | TCGTCGGCAGCGTCCCATGGGAGAAGGAAGGTCAC | 60.0 | 0.01 |
| mm4_intron_Incenp_chr19_9898621_R | GTCTCGTGGGCTCGGGGGTGTGTGTGGGGAGAAAT | 59.8 | 0.01 |
| mm4_intergenic_Kcnc2|Atxn7l3b/Gm26596_chr10_112895334_F | TCGTCGGCAGCGTCCAGATTGCTTCCCTCCACGT | 60.0 | 0.01 |
| mm4_intergenic_Kcnc2|Atxn7l3b/Gm26596_chr10_112895334_R | GTCTCGTGGGCTCGGGGAACGTAGCACAACATGAACT | 59.1 | 0.01 |
| mm4_intergenic_Cpb1|Agtr1b_chr3_20307807_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Cpb1|Agtr1b_chr3_20307807_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Cck|Lyzl4_chr9_121513894_F | TCGTCGGCAGCGTCGGGGCTAGAAGACACTGAACA | 59.3 | 0.01 |
| mm4_intergenic_Cck|Lyzl4_chr9_121513894_R | GTCTCGTGGGCTCGGGTGGTGTGTACATCCTGCCA | 59.9 | 0.01 |
| mm4_intergenic_Nptx2|Gm6272_chr5_144722673_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Nptx2|Gm6272_chr5_144722673_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Snd1|Gm3294_chr6_28961776_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Snd1|Gm3294_chr6_28961776_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Gm13398_chr2_27038088_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Gm13398_chr2_27038088_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm25062|Gm15487_chr6_37615789_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm25062|Gm15487_chr6_37615789_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Ccdc178_chr18_21891778_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Ccdc178_chr18_21891778_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm11207|4930507D10Rik_chr11_80840909_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm11207|4930507D10Rik_chr11_80840909_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm26341|Amd2_chr10_35461338_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm26341|Amd2_chr10_35461338_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_A730060N03Rik|Ift27_chr15_78149876_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_A730060N03Rik|Ift27_chr15_78149876_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_1700123L14Rik|Gm22971_chr6_96364908_F | TCGTCGGCAGCGTCCTTCAGCAAGCCACCTCAAA | 58.6 | 0.00 |
| mm4_intergenic_1700123L14Rik|Gm22971_chr6_96364908_R | GTCTCGTGGGCTCGGCGCCATGATCTCAACCTGGT | 60.1 | 0.00 |
| mm4_intergenic_Vps18|Gm14207_chr2_119307045_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Vps18|Gm14207_chr2_119307045_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_4930583K01Rik|Gm23229_chr7_118279619_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_4930583K01Rik|Gm23229_chr7_118279619_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Pappa2_chr1_158893135_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Pappa2_chr1_158893135_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Dtx4_chr19_12478354_F | TCGTCGGCAGCGTCCATGACCTTCCCCTCAACCC | 60.0 | 0.00 |
| mm4_intron_Dtx4_chr19_12478354_R | GTCTCGTGGGCTCGGCGGGTCTGTTATCCACTGGG | 59.8 | 0.00 |
| mm4_exon_Ttc16_chr2_32774489_F | TCGTCGGCAGCGTCTGACTGGTCCCAAAGATGCG | 60.3 | 0.00 |
| mm4_exon_Ttc16_chr2_32774489_R | GTCTCGTGGGCTCGGCCTTCTGAGCTTACTGCCCC | 60.1 | 0.00 |
| mm4_intron_Lgmn_chr12_102399629_F | TCGTCGGCAGCGTCAGCTCTTGTTCAGTGCCTCC | 59.9 | 0.00 |
| mm4_intron_Lgmn_chr12_102399629_R | GTCTCGTGGGCTCGGGAACCCACACATGCAGGGTA | 59.9 | 0.00 |
| mm4_intron_4930556G01Rik_chr4_30691260_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_4930556G01Rik_chr4_30691260_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Mib1|1010001N08Rik_chr18_10959021_F | TCGTCGGCAGCGTCTTGAGCCAGCGTTCTACAGG | 60.0 | 0.00 |
| mm4_intergenic_Mib1|1010001N08Rik_chr18_10959021_R | GTCTCGTGGGCTCGGTGACACTCCTGCGTCTCAAC | 59.9 | 0.00 |
| mm4_exon_Mark2_chr19_7284567_F | TCGTCGGCAGCGTCTCCTGGATCTCTTCCCGTGT | 59.9 | 0.00 |
| mm4_exon_Mark2_chr19_7284567_R | GTCTCGTGGGCTCGGCACACATGGAGACCAGCCTT | 59.9 | 0.00 |
| mm4_intron_Oxr1_chr15_41483382_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Oxr1_chr15_41483382_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm25672|Barhl2_chr5_106148183_F | TCGTCGGCAGCGTCGCATGAAGAGGAAGTGCCCA | 60.3 | 0.00 |
| mm4_intergenic_Gm25672|Barhl2_chr5_106148183_R | GTCTCGTGGGCTCGGCACAGTCAGGGAGCACTTCT | 59.3 | 0.00 |
| mm4_intergenic_Abl2|Tor3a_chr1_156652505_F | TCGTCGGCAGCGTCAGGGTATTTCCTTTGGGCAGA | 59.0 | 0.00 |
| mm4_intergenic_Abl2|Tor3a_chr1_156652505_R | GTCTCGTGGGCTCGGGAGGAAGGGGCTTGTGACTC | 60.0 | 0.00 |
| mm4_intron_Usp28_chr9_49015851_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Usp28_chr9_49015851_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Tmem50a_chr4_134912361_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Tmem50a_chr4_134912361_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Tbc1d23_chr16_57197967_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Tbc1d23_chr16_57197967_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Spire2_chr8_123357742_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Spire2_chr8_123357742_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Map3k9_chr12_81769633_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Map3k9_chr12_81769633_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Lrmp_chr6_145127101_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Lrmp_chr6_145127101_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Stpg2|mmu-let-7j_chr3_139813673_F | TCGTCGGCAGCGTCTCAGTGATAATTGCTGCGAGGT | 60.0 | 0.00 |
| mm4_intergenic_Stpg2|mmu-let-7j_chr3_139813673_R | GTCTCGTGGGCTCGGGGGCTCCTTGCAGAATGTCT | 60.0 | 0.00 |
| mm4_intergenic_Sox5|Sox5/4933415D12Rik_chr6_143974788_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Sox5|Sox5/4933415D12Rik_chr6_143974788_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Sh3bp4|Agap1_chr1_89385859_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Sh3bp4|Agap1_chr1_89385859_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Rnase1|Ear5_chr14_51148984_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Rnase1|Ear5_chr14_51148984_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Penk/A830012C17Rik|A830012C17Rik_chr4_4185216_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Penk/A830012C17Rik|A830012C17Rik_chr4_4185216_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Hk2|Sema4f_chr6_82881410_F | TCGTCGGCAGCGTCTCTCAAGACCAGGACCCCAA | 60.1 | 0.00 |
| mm4_intergenic_Hk2|Sema4f_chr6_82881410_R | GTCTCGTGGGCTCGGCCAAGAGTGGGCTACTGTGG | 60.0 | 0.00 |
| mm4_intergenic_Gm8177|Tm4sf1_chr3_57159446_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm8177|Tm4sf1_chr3_57159446_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm24175|Tuba3a_chr6_125259927_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm24175|Tuba3a_chr6_125259927_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm11461|Gm11462_chr2_165936731_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm11461|Gm11462_chr2_165936731_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_1700015G11Rik|Gm22211_chr7_52019688_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_1700015G11Rik|Gm22211_chr7_52019688_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Syt2_chr1_134745720_F | TCGTCGGCAGCGTCCACTGCTTTGCCAGTCTTGC | 60.3 | 0.00 |
| mm4_exon_Syt2_chr1_134745720_R | GTCTCGTGGGCTCGGGCGCAAGGAGGTACAGATGT | 60.1 | 0.00 |
| mm4_exon_Efr3a_chr15_65868944_F | TCGTCGGCAGCGTCGTGCACTGCAGGTTTTCAGG | 59.9 | 0.00 |
| mm4_exon_Efr3a_chr15_65868944_R | GTCTCGTGGGCTCGGGCCATGGGCTGGGTATAACA | 59.8 | 0.00 |
| mm3_intron_Scg3_chr9_75654861_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intron_Scg3_chr9_75654861_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_exon_Gm6263_chr13_74019672_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_exon_Gm6263_chr13_74019672_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313949 |
AATTTGTCCGAAGCCGTGAGCAGCCCGCTGCCTGAACCGCCAGAGAAGATGGTGCCCCACGGTTGCCTGAGCCCGCGAGC CGGCCCTCCGACTTCCCGGGAGCGTGGCGGGGGAGGCCAGGAGGAGGAG |
| mm3_intron_Tnr_chr1_159783643 |
TCCTGGCTCACTGATGTCTGCCTGAACCGCCATAGTAAATGGTAGAAACTTCAAGGTGGTTTAGAACACTGTCATCTAAC TGCACTCCATCTGAAAGATGTGCCAAAGAACATGTAGCAAATAGCCACCCTCCAC |
| mm3_intron_Arl15_chr13_114047652 |
TCAACCAGGCTTGTGATTGCCTGAAACATCCCCTCTGGCGGTTCAGGCAGTTTCAGATGGGGGAGTGGAAGGGGTGGGAG GTGCTGCCATTCGGGCTCCAGTCCAGGTTAAC |
| mm4_intergenic_Ndst1|Rps14_chr18_60756540 |
TGAGAGTCTGGTATCCGGCAGCCTGCTAAAGGCTCCATAATGCTACAACTGTGATTCTACTGGATTAAAAATGTTTCCTG AGCCCCAGAACCGCCTGTGAAGAAGGCTTTTCTCCACATGTCTCTCGTGTG |
| mm4_intergenic_Ispd|Mir5099_chr12_36756575 |
ACTGAGCAAGTTCCTAGGCATAGATTTTTTTTTTTGTCATCTTTGAAAATGAAGATGAATAGTTACTTTACCTTCATCCC TGGCAGTTCAGGTCTTCCAAGCTCTTTTCTGTGAGCTCCTGTTGACAACA |
| mm4_intron_Hibadh_chr6_52577439 |
ACCATTCCCAGTTACCTTGTCATTGAATGATTAAATATGAATTCATAATTAGAAAAACCCACACGTAATTTACTGGCTCC TCTGGCGGTTCTGGTTCATTAGCAGTTGATTACTAAACAGTGACCTCTAGTGGCCCAT |
| mm4_intron_D8Ertd82e_chr8_36111939 |
CAGTGCCCACTATCAGGAGGGCAGGATCTTCTATGGCAGTTCAGGTCAGGTAGAGACTTTTCTAGCACTTTATGGGAGAA CCTAGCCTGTGA |
| mm4_intron_Cdk18_chr1_132130848 |
GGTTAGGGCCTGCCTGTAATTTTCAAGGAGAATGTGTTTCCTGGTAAAGAGATAGGACATAGTGCCTTCTCCTCTGTCGG TTCTGGGTCTGGTAGGCAGAAAAGACTCTCCATCTGGGCTGACA |
| mm4_exon_Ddx11_chr17_66150010 |
GACCCACTACAGGTGTGTGGTGATGGTGGGGATGCCATATCCCAACATTAAGTCTCCAGAGCTGCAAGAGAAGATGGCCT ACTTGAATCAGACACTTGTGAGTACCTGTCGTGTTTCAGAGGGCT |
| mm4_intron_2310014F06Rik_chr7_112644687 |
CCCCTCAGCCCCATTCATCAACCCTTTCCTCTGGAGGTTCTGGGTTTCTGTCCCCCATCCCCCACTGTTTATCTTCCAGG CCCTGGAATCACCCAAGCAGAGAGGAGTG |
| mm4_exon_Irs2/9530052E02Rik_chr8_11006613 |
GGGTAAGGGTTGTAGGCCACTTTGGGAGAGGACGCAGGGAAGGATGGGCAGAGGCGACCTGAACTACCAGAGAAGGTGGC CCTCATGAGAGTGTATTCATCTAGAGAGGCAGAGGAAGGCTGAGGTAC |
| mm4_exon_Usp14_chr18_10000555 |
ACCTACTGCATTTGGTTGCTGATTCACTTTTTTATCTTCTAGATCCTTGAATTTTGACCGAAAAGAGACCATTTTCTCTT GAAGTTCTGGTGTGCAGAGTTCATACACATCCA |
| mm4_exon_Ahi1_chr10_20959775 |
CACTACCCAGATCGCACAGGAAATGGAGCCAGAAACTCCAGAGAAGGTGGATTCTGCACAAGAGAAAGTCAGAGGCAAGA CCCCGACAGCAGATGATTCA |
| mm4_exon_Vwa2_chr19_56897900 |
GTTCCCTCCCAGCTTCACCCCCCGCCCCCCATGACTTCTTTCTTGTTTCTCCCCCTGTCCTCAGAGGTGGGCGCACCGAG ACGGGCCTAGCCCTGAAACGCCTGAGCAGAGGGTTCCCCGGAGGCAGAAATGGCTCTGT |
| mm4_intergenic_Gm12494|Gstm3_chr3_107953601 |
GCCGGTGATGAGATCCAGTCCAAACTTTACCATCTTCTGGGGCAGGTCTGGGTATTCAGATACCAAAGGCAGTGATTGGC AGGCCTGCTCTAGTGGCTCTCTGCCTACAGCCCCTCCCCCTGCTGGGTTCTCAGGAAG |
| mm4_intron_Uvssa_chr5_33385251 |
GCCTCCCTCTGTATTGTGCAGAATCCTCTAGGATGCTTGCCAGATTGGGGTGTCCCCAGTCTATCATACTCTCTGGGGGT TCTGGTGAATTGAGTATAGCTTACCCAAGGGTTAGAAACACTGCTTTGAACCACCAGC |
| mm4_intergenic_Siva1|Akt1_chr12_112652422 | CATGGCCACAGGGTCTATCCCCTATCTGCTCTGGTGGTTCAGGCTAGGCCAGGCCTGTCTTCGGGGAGAGGGTA |
| mm4_intergenic_Gm11431|Mrm1_chr11_84792749 |
CTCTCCAGCCAGCCTATGTGAGCATCTTCCCTGGGAGTTCGGGGTAACAGTCCCTGCAGCAGGCTGGGGAGCTTAATCCA CTACTGCATTTACTCCACGTTCCCAATCCCTG |
| mm4_exon_Ttn_chr2_76853903 |
AGCTGGTGCAGGAACTTTCTTCTCTGGTACAATTTTCTTTGGTGGTTCGGGAACTTTGAAAATATTAAGAACATTAGTTT GTATTTTTTTTTTTTTAGGAAGTATCATTATTTGGGTGCCACAACACCCTCTTTGT |
| mm4_intron_Incenp_chr19_9898621 |
CCATGGGAGAAGGAAGGTCACTTCCTCTCCACAAATACTATTTCTGTGCCATTCAGCAGATTAGCAATTGCCATCTCCTC TGGGGGTGCAGGAAATGAACTAAGCATTTCTCCCCACACACACCC |
| mm4_intergenic_Kcnc2|Atxn7l3b/Gm26596_chr10_112895334 |
CAGATTGCTTCCCTCCACGTTGCCCTACCCTCAAGAGAAGATGGTCAATTGCTTTGCCTTTTTAGTTCATGTTGTGCTAC GTTCC |
| mm4_intergenic_Cck|Lyzl4_chr9_121513894 |
GGGGCTAGAAGACACTGAACATCCTCCCAAACCATGGCATGAATTGCTAGGTGAGAAGTAAGAATCCATCTTCTCTGAGG GTACGGGCCCAGAGCAAGTCCTTCATGGCAGGATGTACACACCAC |
| mm4_intergenic_1700123L14Rik|Gm22971_chr6_96364908 |
CTTCAGCAAGCCACCTCAAATACCTTAAGCACCAGAGAAGATGTTTTAGTTGAAGTGACTAGATTAAGTCAGGAAATAAC CAGGTTGAGATCATGGCG |
| mm4_intron_Dtx4_chr19_12478354 |
CATGACCTTCCCCTCAACCCGCCTAAAGCACCAGAGAAAATGGTCTCAAGAGGGAAAACTTACCCAGTGGATAACAGACC CG |
| mm4_exon_Ttc16_chr2_32774489 |
TGACTGGTCCCAAAGATGCGTCGCAGGATCTTCTCTGGGGCTTCAGGAACCTTTAGCTTTGGAGTTTTCTGGGAACGGGC ACTGTGAGGCTCCTGGAACCAAGCCAGGGGCAGTAAGCTCAGAAGG |
| mm4_intron_Lgmn_chr12_102399629 | AGCTCTTGTTCAGTGCCTCCCACACACCACCTTCTCTGGGGTTCCTGGCTACCCTGCATGTGTGGGTTC |
| mm4_intergenic_Mib1|1010001N08Rik_chr18_10959021 |
TTGAGCCAGCGTTCTACAGGCAGGCCAGGAGCCCCAGAGAAAATGGTCCTCTGGATCCAGATGCCTTTCTCAACATGGTT GAGACGCAGGAGTGTCA |
| mm4_exon_Mark2_chr19_7284567 |
TCCTGGATCTCTTCCCGTGTGTAACCCATTGACACCATCAACTCTGGTGGTTGGGGGAGGGGAGGACAGTTAAGGCTGGT CTCCATGTGTG |
| mm4_intergenic_Gm25672|Barhl2_chr5_106148183 |
GCATGAAGAGGAAGTGCCCAGGGAACACAAGGACCAACCAACTCCTCTGGGGGTTGGGGGTCAAGGAAGAAGTGCTCCCT GACTGTG |
| mm4_intergenic_Abl2|Tor3a_chr1_156652505 |
AGGGTATTTCCTTTGGGCAGAACCATTTTCTCTGGGGGTGGGGGTGAGAAGCTCATATAGCTTAGAAAGAGTCACAAGCC CCTTCCTC |
| mm4_intergenic_Stpg2|mmu-let-7j_chr3_139813673 |
TCAGTGATAATTGCTGCGAGGTAAAAATGTCTTTCATTCCTGAGCAGCCAGAGAAGAGAGTAGCAGTGATAGCTTGATGA CATGAGACATTCTGCAAGGAGCCC |
| mm4_intergenic_Hk2|Sema4f_chr6_82881410 |
TCTCAAGACCAGGACCCCAAACCATCTTCTCTGCCTCTCCAGGGGCCTGCACTAAGGGGCCAGCCAGCCAGTCAGGGGAA CGCCACAGTAGCCCACTCTTGG |
| mm4_exon_Syt2_chr1_134745720 |
CACTGCTTTGCCAGTCTTGCCGACATGGGGTCAGCCACACTAGAGGCCATTGGATAGCTGAAGTGACCTAAATCCTGCTT CTTTCCCTGGACAGCCAGAGAAGTTGGGTGACATCTGTACCTCCTTGCGC |
| mm4_exon_Efr3a_chr15_65868944 |
GTGCACTGCAGGTTTTCAGGCCTGTCTGCACATTTTCTCCTGATCAGCCAGAGAAGAGAGTCTATGCTCTACAAGACTGA AGCAGAACCATGTTATACCCAGCCCATGGC |