CRISPOR

DNA break repair outcome predictions

Guide: GCTCCTAATCCCAGGCTCCC TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GGGGGAAAACCCCCAAGTAAGCTCCTAATCCCAGGCTCCCTGGGATCTTGGCTGAGAAGAGGAGCTAAGGCGACAAGGCA`	(Wild-type sequence)
314	`CCCCAAGTAAGCTCCTAATCCCAGGCT----------------GAGAAGAGGAGCTAAGG`	`16 bp deleted → frameshift`
268	`CCCCAAGTAAGCTCCTAATCCCAGG--------GATCTTGGCTGAGAAGAGGAGCTAAGG`	`8 bp deleted → frameshift`
255	`CCCCAAGTAAGCTCCTAATCCCAGG---------ATCTTGGCTGAGAAGAGGAGCTAAGG`	`9 bp deleted → no frameshift`
191	`CCCCAAGTAAGCTCCTAATCCCAGGCTC---------TTGGCTGAGAAGAGGAGCTAAGG`	`9 bp deleted → no frameshift`
173	`CCCCAAGTAAGCTCCTAATCCCAGGCT-----------TGGCTGAGAAGAGGAGCTAAGG`	`11 bp deleted → frameshift`
170	`CCCCAAGTAAGCTCCTAATC-----------------TTGGCTGAGAAGAGGAGCTAAGG`	`17 bp deleted → frameshift`
129	`CCCCAAGTAAGCTCCTAATCCCAGG---------------------------AGCTAAGG`	`27 bp deleted → no frameshift`
117	`CCCCAAGTAAGCTCCTAATCCCAGGCT-----------------------------AAGG`	`29 bp deleted → frameshift`
111	`CCCCAAGTAAGCT------------------------------GAGAAGAGGAGCTAAGG`	`30 bp deleted → no frameshift`
99	`CCCCAAGTAAGCTCCTAATCCCAG----------------------AAGAGGAGCTAAGG`	`22 bp deleted → frameshift`
99	`CCCCAAGTAAGCTCCT----------------------TGGCTGAGAAGAGGAGCTAAGG`	`22 bp deleted → frameshift`
95	`CCCCAAGTAAGCTC-----------------------TTGGCTGAGAAGAGGAGCTAAGG`	`23 bp deleted → frameshift`
87	`CCCCAAGTAAGCTCCTAATCCCAGG-----------------------------------`	`35 bp deleted → frameshift`
86	`CCCCAAGTAAGCTCCTAATCCCAG-------------------------AGGAGCTAAGG`	`25 bp deleted → frameshift`
86	`CCCCAAGTAAGCT-------------------------TGGCTGAGAAGAGGAGCTAAGG`	`25 bp deleted → frameshift`
77	`CCCCAAGTAAGCTCCT---------------------------GAGAAGAGGAGCTAAGG`	`27 bp deleted → no frameshift`
69	`CCCCAAGTAAGCT-------------------------------------------AAGG`	`43 bp deleted → frameshift`
67	`CCCCAAGTAAGCTCCTAA----------------------------------------GG`	`40 bp deleted → frameshift`
66	`CCCCAAGTAAGCTCCTAATCCCAG------------------------------CTAAGG`	`30 bp deleted → no frameshift`
60	`CCCCAAGTAAG--------------------------------------AGGAGCTAAGG`	`38 bp deleted → frameshift`
52	`CCCCAAGTAAG-----------------------------------AAGAGGAGCTAAGG`	`35 bp deleted → frameshift`
48	`CCCCAAG------------------------------------------AGGAGCTAAGG`	`42 bp deleted → no frameshift`
45	`CCCCAAGTAAG------------------------------------------------G`	`48 bp deleted → no frameshift`
44	`CCCCAAGTAAGCTCCTAA------------------------------GAGGAGCTAAGG`	`30 bp deleted → no frameshift`
42	`CCCCAAG---------------------------------------AAGAGGAGCTAAGG`	`39 bp deleted → no frameshift`
40	`CCCCAAGTAAG----------------------------------------GAGCTAAGG`	`40 bp deleted → frameshift`
33	`CCCCAAG--------------------------------------------GAGCTAAGG`	`44 bp deleted → frameshift`
29	`CCCCAAG----------------------------------------------------G`	`52 bp deleted → frameshift`
28	`CCCCAAG-----------------------------------------------CTAAGG`	`47 bp deleted → frameshift`

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