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Contents:

PCR primers for off-targets of GGCCTCCCCCGCCACGCTCC CGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314014_F TCGTCGGCAGCGTCAACCGCCAGAGAAGATGGTG 60.0 1.00
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314014_R GTCTCGTGGGCTCGGCTGCCTGCTAGTCCATCGAC 60.2 1.00
mm4_intron_Ttc4_chr4_106663284_F Primer3: not found at this Tm N.d. 0.68
mm4_intron_Ttc4_chr4_106663284_R Primer3: not found at this Tm N.d. 0.68
mm4_intergenic_Cox4i1|Gm24239_chr8_120696610_F Primer3: not found at this Tm N.d. 0.54
mm4_intergenic_Cox4i1|Gm24239_chr8_120696610_R Primer3: not found at this Tm N.d. 0.54
mm4_intron_Spag17_chr3_100044455_F Primer3: not found at this Tm N.d. 0.54
mm4_intron_Spag17_chr3_100044455_R Primer3: not found at this Tm N.d. 0.54
mm4_intron_Cacna1b_chr2_24728433_F Primer3: not found at this Tm N.d. 0.54
mm4_intron_Cacna1b_chr2_24728433_R Primer3: not found at this Tm N.d. 0.54
mm4_intron_4930401O12Rik_chr13_31218867_F Primer3: not found at this Tm N.d. 0.54
mm4_intron_4930401O12Rik_chr13_31218867_R Primer3: not found at this Tm N.d. 0.54
mm4_intergenic_Gm9621|Gm24200_chr9_94235438_F Primer3: not found at this Tm N.d. 0.54
mm4_intergenic_Gm9621|Gm24200_chr9_94235438_R Primer3: not found at this Tm N.d. 0.54
mm4_intergenic_Cacna1b|Ehmt1_chr2_24784636_F Primer3: not found at this Tm N.d. 0.54
mm4_intergenic_Cacna1b|Ehmt1_chr2_24784636_R Primer3: not found at this Tm N.d. 0.54
mm3_exon_Obscn_chr11_59135720_F TCGTCGGCAGCGTCTATGCCCTCTGCGCGCAT 62.2 0.39
mm3_exon_Obscn_chr11_59135720_R GTCTCGTGGGCTCGGCTCTGGTGGTGGACTCGGA 60.6 0.39
mm4_intron_Adipoq_chr16_23147933_F Primer3: not found at this Tm N.d. 0.38
mm4_intron_Adipoq_chr16_23147933_R Primer3: not found at this Tm N.d. 0.38
mm3_intergenic_Gm11523|Cdk5rap3_chr11_96886125_F TCGTCGGCAGCGTCTTCGCTTCAGCCTCACAAGG 60.6 0.30
mm3_intergenic_Gm11523|Cdk5rap3_chr11_96886125_R GTCTCGTGGGCTCGGCTGCCTGCAGTTACCAAGCT 60.6 0.30
mm4_intergenic_Mir3072/Mirg|Dio3os_chr12_109886811_F Primer3: not found at this Tm N.d. 0.29
mm4_intergenic_Mir3072/Mirg|Dio3os_chr12_109886811_R Primer3: not found at this Tm N.d. 0.29
mm4_intron_Tjp1_chr7_65370530_F Primer3: not found at this Tm N.d. 0.27
mm4_intron_Tjp1_chr7_65370530_R Primer3: not found at this Tm N.d. 0.27
mm4_intron_Shc3_chr13_51469116_F TCGTCGGCAGCGTCGAAGTGGTTTGTGCTGGCTG 59.9 0.26
mm4_intron_Shc3_chr13_51469116_R GTCTCGTGGGCTCGGTCTGAAGGCTTGGAGAGTGC 59.6 0.26
mm4_intergenic_Ifng|Dyrk2/4932442E05Rik_chr10_118805201_F Primer3: not found at this Tm N.d. 0.25
mm4_intergenic_Ifng|Dyrk2/4932442E05Rik_chr10_118805201_R Primer3: not found at this Tm N.d. 0.25
mm4_intron_Gramd1b_chr9_40398285_F Primer3: not found at this Tm N.d. 0.25
mm4_intron_Gramd1b_chr9_40398285_R Primer3: not found at this Tm N.d. 0.25
mm3_intergenic_Mpp5|Gm25225_chr12_78749159_F Primer3: not found at this Tm N.d. 0.23
mm3_intergenic_Mpp5|Gm25225_chr12_78749159_R Primer3: not found at this Tm N.d. 0.23
mm2_intergenic_Crhr2|Inmt_chr6_55142201_F Primer3: not found at this Tm N.d. 0.22
mm2_intergenic_Crhr2|Inmt_chr6_55142201_R Primer3: not found at this Tm N.d. 0.22
mm4_intergenic_Dis3l2|Alppl2_chr1_87056406_F Primer3: not found at this Tm N.d. 0.22
mm4_intergenic_Dis3l2|Alppl2_chr1_87056406_R Primer3: not found at this Tm N.d. 0.22
mm4_exon_Tsc22d2_chr3_58416019_F TCGTCGGCAGCGTCCCGAACCTCATCCTGGATGG 59.8 0.21
mm4_exon_Tsc22d2_chr3_58416019_R GTCTCGTGGGCTCGGGGAGTGGACACCGAAGCG 60.4 0.21
mm4_exon_Zbtb7a_chr10_81144245_F TCGTCGGCAGCGTCTGGACCAGCAGAACGTGTAC 59.9 0.21
mm4_exon_Zbtb7a_chr10_81144245_R GTCTCGTGGGCTCGGCTCAGGATGTCGCCCACATT 60.1 0.21
mm3_exon_Hmcn1_chr1_150993403_F TCGTCGGCAGCGTCGCAGGGAGCTGCGAGTAG 59.8 0.21
mm3_exon_Hmcn1_chr1_150993403_R GTCTCGTGGGCTCGGAAAGTTGGGAGGCTCAGTCG 59.9 0.21
mm4_intergenic_mmu-mir-6380|H2afz_chr3_137846774_F Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_mmu-mir-6380|H2afz_chr3_137846774_R Primer3: not found at this Tm N.d. 0.21
mm4_exon_Ltb_chr17_35196232_F TCGTCGGCAGCGTCTGTGAATCGTGATGTCCGGG 60.1 0.21
mm4_exon_Ltb_chr17_35196232_R GTCTCGTGGGCTCGGTTCCGTGAAGACACTGGCAA 59.8 0.21
mm4_intron_Pmfbp1_chr8_109523044_F Primer3: not found at this Tm N.d. 0.18
mm4_intron_Pmfbp1_chr8_109523044_R Primer3: not found at this Tm N.d. 0.18
mm4_intron_Crtc1_chr8_70420527_F TCGTCGGCAGCGTCGGTCAAAGCCACAGCAAAGG 59.9 0.15
mm4_intron_Crtc1_chr8_70420527_R GTCTCGTGGGCTCGGGCCTCTTTCCTGCGACTGT 60.0 0.15
mm3_exon_Gm12963_chr4_130047705_F Primer3: not found at this Tm N.d. 0.15
mm3_exon_Gm12963_chr4_130047705_R Primer3: not found at this Tm N.d. 0.15
mm4_intron_Alpk3_chr7_81094191_F Primer3: not found at this Tm N.d. 0.14
mm4_intron_Alpk3_chr7_81094191_R Primer3: not found at this Tm N.d. 0.14
mm4_exon_Aldh1a2_chr9_71215884_F TCGTCGGCAGCGTCCCCATGACTTCCAGCGAGAT 59.5 0.13
mm4_exon_Aldh1a2_chr9_71215884_R GTCTCGTGGGCTCGGACTTGATCTCGAGGTTGGGC 59.7 0.13
mm4_intron_Dlg1_chr16_31833903_F Primer3: not found at this Tm N.d. 0.13
mm4_intron_Dlg1_chr16_31833903_R Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Adamts6|Cwc27_chr13_104510295_F Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Adamts6|Cwc27_chr13_104510295_R Primer3: not found at this Tm N.d. 0.13
mm4_exon_Naa25/Erp29_chr5_121440553_F Primer3: not found at this Tm N.d. 0.13
mm4_exon_Naa25/Erp29_chr5_121440553_R Primer3: not found at this Tm N.d. 0.13
mm4_intron_Tprg_chr16_25368085_F TCGTCGGCAGCGTCGCTCCAGCAACATCTCCTGT 60.0 0.12
mm4_intron_Tprg_chr16_25368085_R GTCTCGTGGGCTCGGTAACAGCATCCACACCTCCC 59.3 0.12
mm4_exon_Phf15_chr11_51857442_F TCGTCGGCAGCGTCGCTCCCGAAGCCAGGAGC 62.8 0.12
mm4_exon_Phf15_chr11_51857442_R GTCTCGTGGGCTCGGCTGACCATCTTCCCAGCAGG 60.1 0.12
mm4_intergenic_Gm25199|Syt6_chr3_103575160_F TCGTCGGCAGCGTCGAACCTGCCCGGACTCAG 59.7 0.12
mm4_intergenic_Gm25199|Syt6_chr3_103575160_R GTCTCGTGGGCTCGGGAGGCTCCGCTCTGCTTTTA 60.1 0.12
mm4_exon_Cnnm1_chr19_43440895_F TCGTCGGCAGCGTCGACGTGGAGGTCCTGGGG 61.3 0.12
mm4_exon_Cnnm1_chr19_43440895_R GTCTCGTGGGCTCGGCACCCGCACCTGGACCAG 62.7 0.12
mm4_intergenic_Actrt2|Gm13113_chr4_154701181_F TCGTCGGCAGCGTCCTTCCCAGAGAGACCGCAAG 60.1 0.11
mm4_intergenic_Actrt2|Gm13113_chr4_154701181_R GTCTCGTGGGCTCGGATCGCTCACACACCAAGGAG 60.0 0.11
mm4_intergenic_Zg16|Gm22747_chr7_127055684_F TCGTCGGCAGCGTCCCCCAGGCAACACTTCATTTC 59.7 0.11
mm4_intergenic_Zg16|Gm22747_chr7_127055684_R GTCTCGTGGGCTCGGACCAATGGGAAATTTAAACGAGAT 57.1 0.11
mm4_exon_Gm10760/Mctp1_chr13_76384444_F TCGTCGGCAGCGTCCTCCGAGCTGGCCTGAAG 59.8 0.11
mm4_exon_Gm10760/Mctp1_chr13_76384444_R GTCTCGTGGGCTCGGTTGCCTTTCCTCCTCCCTCT 60.1 0.11
mm4_intergenic_Zic1|Zic4/Zic1_chr9_91361962_F TCGTCGGCAGCGTCAATGGGACTTGGGCTTGGTT 59.8 0.11
mm4_intergenic_Zic1|Zic4/Zic1_chr9_91361962_R GTCTCGTGGGCTCGGAAAAGCAGCCTCCTCCTTCC 59.9 0.11
mm4_exon_Rd3/Gm26670_chr1_191985444_F TCGTCGGCAGCGTCACCATCTCGGAGGATGTGGA 60.0 0.11
mm4_exon_Rd3/Gm26670_chr1_191985444_R GTCTCGTGGGCTCGGCCAGCCCGTTATTCCTCCTG 60.1 0.11
mm4_intergenic_Gm14513|Gm26314_chrX_11714054_F TCGTCGGCAGCGTCGGGAGCATTTGTTGAAGCGA 59.1 0.09
mm4_intergenic_Gm14513|Gm26314_chrX_11714054_R GTCTCGTGGGCTCGGGGGCCTTTCTGTGTTTCAGG 59.0 0.09
mm4_exon_Mtss1l_chr8_110721568_F Primer3: not found at this Tm N.d. 0.09
mm4_exon_Mtss1l_chr8_110721568_R Primer3: not found at this Tm N.d. 0.09
mm4_intron_Sez6_chr11_77931462_F TCGTCGGCAGCGTCTCCACTCCAGGCGTCTAGG 60.3 0.09
mm4_intron_Sez6_chr11_77931462_R GTCTCGTGGGCTCGGTCGAGCGAAGTTTGGAGACC 60.0 0.09
mm4_intergenic_Cops8|Gm9991_chr1_90644089_F TCGTCGGCAGCGTCGTCTTGCGCAGTCCAATGTG 60.1 0.08
mm4_intergenic_Cops8|Gm9991_chr1_90644089_R GTCTCGTGGGCTCGGCCCCTGGATTGCATACTCCC 59.8 0.08
mm4_intron_Rxra_chr2_27677639_F TCGTCGGCAGCGTCGTGGCGGTACCGAGAGTTAG 59.9 0.08
mm4_intron_Rxra_chr2_27677639_R GTCTCGTGGGCTCGGGCTCCTCGGCAAGTCAGAG 60.1 0.08
mm4_intergenic_Smyd2|Prox1_chr1_189922419_F TCGTCGGCAGCGTCGAACTCGGCGCTCTGCTC 60.8 0.08
mm4_intergenic_Smyd2|Prox1_chr1_189922419_R GTCTCGTGGGCTCGGCTCCAGCCTTCCTCACCG 59.4 0.08
mm4_intron_Arhgef37_chr18_61499466_F TCGTCGGCAGCGTCGGTTTGCCTTGCTTGCACAG 60.8 0.08
mm4_intron_Arhgef37_chr18_61499466_R GTCTCGTGGGCTCGGTGGTGCCAGTCAGCTTACAG 59.9 0.08
mm4_intron_Trio_chr15_27856468_F Primer3: not found at this Tm N.d. 0.08
mm4_intron_Trio_chr15_27856468_R Primer3: not found at this Tm N.d. 0.08
mm4_exon_Gm7701_chr18_24258301_F TCGTCGGCAGCGTCGGTTGTTCAGGCCCATGAGA 59.9 0.07
mm4_exon_Gm7701_chr18_24258301_R GTCTCGTGGGCTCGGCGGAGGTCTCATCTGTCACG 59.9 0.07
mm4_intergenic_Krtap5-5|Gm2431_chr7_142230432_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Krtap5-5|Gm2431_chr7_142230432_R Primer3: not found at this Tm N.d. 0.07
mm4_exon_Slc25a36_chr9_97076360_F Primer3: not found at this Tm N.d. 0.07
mm4_exon_Slc25a36_chr9_97076360_R Primer3: not found at this Tm N.d. 0.07
mm3_intron_Jarid2_chr13_44730342_F TCGTCGGCAGCGTCCTCCAACCCCTCGCTGAC 59.7 0.07
mm3_intron_Jarid2_chr13_44730342_R GTCTCGTGGGCTCGGCGCCTGTGGAATCGAGGAAT 60.1 0.07
mm4_intron_C1qtnf1_chr11_118428804_F Primer3: not found at this Tm N.d. 0.07
mm4_intron_C1qtnf1_chr11_118428804_R Primer3: not found at this Tm N.d. 0.07
mm4_intron_Zc3h8_chr2_128943786_F TCGTCGGCAGCGTCCTGACTTCCACCCCACTTCC 59.9 0.07
mm4_intron_Zc3h8_chr2_128943786_R GTCTCGTGGGCTCGGGTGATCCCCACGTTCTGGTC 60.3 0.07
mm4_exon_Setd8_chr5_124445495_F Primer3: not found at this Tm N.d. 0.07
mm4_exon_Setd8_chr5_124445495_R Primer3: not found at this Tm N.d. 0.07
mm4_exon_Ndnl2_chr7_64872848_F TCGTCGGCAGCGTCGACGTGCTGGGTCCTTCC 60.0 0.07
mm4_exon_Ndnl2_chr7_64872848_R GTCTCGTGGGCTCGGGACATGTTGCAGAAGCCGAG 59.5 0.07
mm4_intron_Gm11762_chr11_119565558_F TCGTCGGCAGCGTCATGAAGCTGCTGTCCCTCAC 60.0 0.07
mm4_intron_Gm11762_chr11_119565558_R GTCTCGTGGGCTCGGGGCAGACCTCATGCTTCTGT 60.0 0.07
mm4_intergenic_Capn9|2310022B05Rik_chr8_124631984_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Capn9|2310022B05Rik_chr8_124631984_R Primer3: not found at this Tm N.d. 0.06
mm2_exon_Clu_chr14_65974705_F Primer3: not found at this Tm N.d. 0.06
mm2_exon_Clu_chr14_65974705_R Primer3: not found at this Tm N.d. 0.06
mm4_exon_Trim41_chr11_48816993_F Primer3: not found at this Tm N.d. 0.06
mm4_exon_Trim41_chr11_48816993_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_B230219D22Rik_chr13_55693577_F TCGTCGGCAGCGTCAGTCTCGCCCTGGAGCTG 61.0 0.06
mm4_intron_B230219D22Rik_chr13_55693577_R GTCTCGTGGGCTCGGTCTTCTCTGCGCTCCTTAGC 59.5 0.06
mm4_intergenic_Fam49b|Gm25628_chr15_63997974_F TCGTCGGCAGCGTCCTCGTCACGGCGCTAGGG 62.5 0.06
mm4_intergenic_Fam49b|Gm25628_chr15_63997974_R GTCTCGTGGGCTCGGCTGTCGCCTGGCTCTTCG 60.5 0.06
mm4_intergenic_Lzts3|Ddrgk1_chr2_130642834_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Lzts3|Ddrgk1_chr2_130642834_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Rhobtb3|Gm6311_chr13_75943948_F TCGTCGGCAGCGTCTCGGAAGGCTGAAACCTGAG 59.6 0.05
mm4_intergenic_Rhobtb3|Gm6311_chr13_75943948_R GTCTCGTGGGCTCGGACACACTTTCTGCGAAGGCT 60.1 0.05
mm4_intron_Nek7_chr1_138618062_F TCGTCGGCAGCGTCGGCCCACATCCTCAGACAAA 59.9 0.05
mm4_intron_Nek7_chr1_138618062_R GTCTCGTGGGCTCGGGGAGGGCGGGTACTTCCTA 60.0 0.05
mm4_intron_Ehd2_chr7_15955204_F TCGTCGGCAGCGTCGGGAGGCGTGGCTTGTTATT 60.6 0.04
mm4_intron_Ehd2_chr7_15955204_R GTCTCGTGGGCTCGGCGCCTGCCTCTTTCTGCTAT 60.1 0.04
mm4_intergenic_Klf14|Mir29a_chr6_30959067_F TCGTCGGCAGCGTCTGGCGAGCCAGGTAATTTCA 59.6 0.04
mm4_intergenic_Klf14|Mir29a_chr6_30959067_R GTCTCGTGGGCTCGGTAGGACTCTGCCTGACACCC 60.6 0.04
mm4_intergenic_Prmt6|Gm26076_chr3_110364511_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Prmt6|Gm26076_chr3_110364511_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gli2|mmu-mir-6346_chr1_118927093_F TCGTCGGCAGCGTCTGCAGGTTACAGGAGCAAGG 59.9 0.04
mm4_intergenic_Gli2|mmu-mir-6346_chr1_118927093_R GTCTCGTGGGCTCGGAATGCCCACCAGGAGAAAGG 59.9 0.04
mm4_intron_Sirpa_chr2_129594922_F TCGTCGGCAGCGTCGCTTCCTCCTGCCTTACCAG 60.1 0.04
mm4_intron_Sirpa_chr2_129594922_R GTCTCGTGGGCTCGGAATCTGGGCCCAGTGTTCTG 59.9 0.04
mm4_intergenic_Tcte2|Gm24867_chr17_13667284_F TCGTCGGCAGCGTCCTGAGAACACAGAGGCTGGG 60.0 0.03
mm4_intergenic_Tcte2|Gm24867_chr17_13667284_R GTCTCGTGGGCTCGGAAATCAAGGCTGGTCTGGGG 59.9 0.03
mm4_intergenic_Zeb2/Gm13476/ZEB2_AS1_3/ZEB2_AS1_4|Zeb2_chr2_45117167_F TCGTCGGCAGCGTCGAACTTCGCTCCCTCTGTCG 60.4 0.03
mm4_intergenic_Zeb2/Gm13476/ZEB2_AS1_3/ZEB2_AS1_4|Zeb2_chr2_45117167_R GTCTCGTGGGCTCGGGCGAGCCAGGTCAGCTGA 62.0 0.03
mm4_intergenic_Gm8614|Gm23335_chr18_55842307_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm8614|Gm23335_chr18_55842307_R Primer3: not found at this Tm N.d. 0.03
mm4_exon_Glcci1/A430035B10Rik_chr6_8509977_F Primer3: not found at this Tm N.d. 0.03
mm4_exon_Glcci1/A430035B10Rik_chr6_8509977_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Gm16126_chr9_95646579_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Gm16126_chr9_95646579_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Sdccag8|Gm15423_chr1_176927625_F TCGTCGGCAGCGTCTGTGCAATGAACATCGACGC 59.8 0.03
mm4_intergenic_Sdccag8|Gm15423_chr1_176927625_R GTCTCGTGGGCTCGGACGCTCTTGGTTTCCTCTGG 59.9 0.03
mm4_intron_Gtf2a1_chr12_91589290_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Gtf2a1_chr12_91589290_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Zfr_chr15_12118808_F TCGTCGGCAGCGTCATGGAGAGAGAGTGAGCCGA 59.7 0.03
mm4_intron_Zfr_chr15_12118808_R GTCTCGTGGGCTCGGGGTACCGACAAAGACCTGCT 59.6 0.03
mm4_exon_D030062O11Rik/Tbx18_chr9_87730405_F TCGTCGGCAGCGTCCAGAGAGCCGCGGATGGA 61.5 0.02
mm4_exon_D030062O11Rik/Tbx18_chr9_87730405_R GTCTCGTGGGCTCGGGAACCTGAGCTTCGGTGAGG 60.3 0.02
mm3_exon_Surf4_chr2_26933509_F TCGTCGGCAGCGTCGGCCAAGCCTTAAAGGGACC 60.9 0.02
mm3_exon_Surf4_chr2_26933509_R GTCTCGTGGGCTCGGCTGTTCGTGAAGGGCGGT 59.9 0.02
mm4_intergenic_Lingo1|Odf3l1_chr9_56825697_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Lingo1|Odf3l1_chr9_56825697_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_n-R5s179|4930505K14Rik_chr5_148765935_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_n-R5s179|4930505K14Rik_chr5_148765935_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Grhl3_chr4_135564040_F TCGTCGGCAGCGTCTGAGCCTGGGGTTAGAGGAA 59.8 0.02
mm4_intron_Grhl3_chr4_135564040_R GTCTCGTGGGCTCGGGTCCCTTAGACACCCCTTGC 60.0 0.02
mm4_intron_Ryr1_chr7_29090289_F TCGTCGGCAGCGTCAAGACAACCCAACCGTGGTT 60.0 0.02
mm4_intron_Ryr1_chr7_29090289_R GTCTCGTGGGCTCGGGTGGTGGGTGTTTGTGTGTG 59.8 0.02
mm4_intergenic_Psma7|Ss18l1_chr2_180042436_F TCGTCGGCAGCGTCCTCAAAAGCGCACACTCACC 60.0 0.02
mm4_intergenic_Psma7|Ss18l1_chr2_180042436_R GTCTCGTGGGCTCGGTGCTGAGCTTCGGTGGTTC 60.3 0.02
mm3_intergenic_Tnip3|Ndnf_chr6_65670122_F Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Tnip3|Ndnf_chr6_65670122_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Prkacb_chr3_146812869_F Primer3: not found at this Tm N.d. 0.01
mm4_exon_Prkacb_chr3_146812869_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Fbxo5|Mtrf1l_chr10_5808662_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Fbxo5|Mtrf1l_chr10_5808662_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Nrg2_chr18_36196031_F TCGTCGGCAGCGTCACTTGTAAGCGGAGGCCTTC 60.0 0.01
mm4_intron_Nrg2_chr18_36196031_R GTCTCGTGGGCTCGGTGAGGCTCAGTGAGTCAGGA 59.8 0.01
mm4_intergenic_Enox1|Gm10132_chr14_77169569_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Enox1|Gm10132_chr14_77169569_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Ankfn1_chr11_89538111_F TCGTCGGCAGCGTCGCTCTTCGTGTGACTGAGCT 60.0 0.01
mm4_intron_Ankfn1_chr11_89538111_R GTCTCGTGGGCTCGGGCCGTCTGAGATAGTGCACA 59.8 0.01
mm4_intron_Celsr1_chr15_86029994_F TCGTCGGCAGCGTCCCCCACAAACCTGCTGAAGA 60.1 0.01
mm4_intron_Celsr1_chr15_86029994_R GTCTCGTGGGCTCGGTTGCTGCTGTGATTGGTTGC 59.9 0.01
mm4_exon_Gm12462/Pax5_chr4_44684618_F TCGTCGGCAGCGTCTTCTCTTTCCCCGAAGCCTG 59.6 0.01
mm4_exon_Gm12462/Pax5_chr4_44684618_R GTCTCGTGGGCTCGGGTGCCATGCTAGTGGTGTCT 60.0 0.01
mm4_intergenic_Gm24338|Ppp2r3a_chr9_101251414_F TCGTCGGCAGCGTCTCCTGTTAACGAGTTCCCGG 59.3 0.01
mm4_intergenic_Gm24338|Ppp2r3a_chr9_101251414_R GTCTCGTGGGCTCGGCCACGCCAGAGGACTCCC 61.7 0.01
mm4_exon_Tbkbp1_chr11_97138893_F TCGTCGGCAGCGTCGTAGAGCTCGCCGCCATAG 60.0 0.01
mm4_exon_Tbkbp1_chr11_97138893_R GTCTCGTGGGCTCGGCAGCTACTCGGAGCTGGC 59.8 0.01
mm4_intergenic_Gm23681|Peli1_chr11_21034952_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm23681|Peli1_chr11_21034952_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Macrod1_chr19_7062344_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Macrod1_chr19_7062344_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_3425401B19Rik|1810011H11Rik_chr14_32704240_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_3425401B19Rik|1810011H11Rik_chr14_32704240_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_C130040N14Rik/AI413582|Nudt3_chr17_27574903_F TCGTCGGCAGCGTCCTTGCAGAGCCAGAGAGGAC 60.1 0.00
mm4_intergenic_C130040N14Rik/AI413582|Nudt3_chr17_27574903_R GTCTCGTGGGCTCGGTTCCTCTCACTCGCTCTTGC 59.7 0.00
mm4_intergenic_Atp1a1|Mab21l3_chr3_101797617_F TCGTCGGCAGCGTCGGTCCAAAGAGGGTGGTAGG 59.3 0.00
mm4_intergenic_Atp1a1|Mab21l3_chr3_101797617_R GTCTCGTGGGCTCGGCCAGGCTGCATGAGGATGAA 60.1 0.00
mm4_intron_Fendrr_chr8_121070362_F TCGTCGGCAGCGTCTGGAAGCTCTTCTGCTCAGC 60.0 0.00
mm4_intron_Fendrr_chr8_121070362_R GTCTCGTGGGCTCGGGGGTTGCTGGGAGGAATGAA 59.9 0.00
mm4_exon_Apbb1_chr7_105574105_F TCGTCGGCAGCGTCGCATTAAGCCTTTGGGACCC 59.1 0.00
mm4_exon_Apbb1_chr7_105574105_R GTCTCGTGGGCTCGGCACCGAGACCAGAACCGAAA 59.9 0.00
mm4_exon_Fbxo9_chr9_78108320_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Fbxo9_chr9_78108320_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Ccne2_chr4_11192342_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Ccne2_chr4_11192342_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Sdc4_chr2_164435894_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Sdc4_chr2_164435894_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm10612/Cbfa2t3|Acsf3_chr8_122738273_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm10612/Cbfa2t3|Acsf3_chr8_122738273_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Egr1_chr18_34860943_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Egr1_chr18_34860943_R Primer3: not found at this Tm N.d. 0.00
mm3_exon_Clk3_chr9_57765258_F TCGTCGGCAGCGTCCGAACTCGCAGACTCTGGG 60.1 0.00
mm3_exon_Clk3_chr9_57765258_R GTCTCGTGGGCTCGGGGAAGAGACCTCCACCGATG 59.5 0.00
mm4_intergenic_Gm13002|Wnt4_chr4_137277310_F TCGTCGGCAGCGTCCTCTAGCTCGGCCCAACTG 59.8 0.00
mm4_intergenic_Gm13002|Wnt4_chr4_137277310_R GTCTCGTGGGCTCGGACTGGGCTTCTCCTGGGG 60.6 0.00
mm4_exon_Nup153_chr13_46727537_F TCGTCGGCAGCGTCCCCCTCCTATGCCTCCGG 60.9 0.00
mm4_exon_Nup153_chr13_46727537_R GTCTCGTGGGCTCGGTCTCGCTGTGTCTGTGTGTC 59.9 0.00
mm4_intergenic_Krt40|Krtap3-3_chr11_99546878_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Krt40|Krtap3-3_chr11_99546878_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Plekhm3_chr1_64845276_F TCGTCGGCAGCGTCGACCCGTTGTTCCTGTCCTT 59.8 0.00
mm4_intron_Plekhm3_chr1_64845276_R GTCTCGTGGGCTCGGGCAAACTGCCACCAAGATCC 59.7 0.00
mm4_intron_Prr14l_chr5_32839003_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Prr14l_chr5_32839003_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Nphp4_chr4_152556601_F TCGTCGGCAGCGTCGCAGCCTGGATCCTTGTCTT 60.0 0.00
mm4_intron_Nphp4_chr4_152556601_R GTCTCGTGGGCTCGGGACCTATGCCTTCCCTGCTG 60.1 0.00
mm4_intron_Kcnn2_chr18_45675601_F TCGTCGGCAGCGTCCCAACTCTCAGATGGCACCA 59.6 0.00
mm4_intron_Kcnn2_chr18_45675601_R GTCTCGTGGGCTCGGACAGCAGGAAGAGATGCAGA 58.7 0.00
mm4_intron_Grik4_chr9_42733745_F TCGTCGGCAGCGTCCCACTGTTGGCTGAGCATAAG 59.2 0.00
mm4_intron_Grik4_chr9_42733745_R GTCTCGTGGGCTCGGGCTCTGAGCCCATGGATCTC 59.9 0.00
mm4_intron_Grhl2_chr15_37344144_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Grhl2_chr15_37344144_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Fbxo21_chr5_117977099_F TCGTCGGCAGCGTCGAGGTGAGCGAGCATCCC 59.8 0.00
mm4_intron_Fbxo21_chr5_117977099_R GTCTCGTGGGCTCGGGCAGGCAGGCCTTTGTCT 60.2 0.00
mm4_intron_Fam129b_chr2_32881258_F TCGTCGGCAGCGTCCTCCCACCCTTTAGCCTGTG 60.0 0.00
mm4_intron_Fam129b_chr2_32881258_R GTCTCGTGGGCTCGGTCTCTTTTGGCAGCCTGTGT 59.8 0.00
mm4_intron_Dst_chr1_34011800_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Dst_chr1_34011800_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Tjp1|Gm7546_chr7_65572837_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Tjp1|Gm7546_chr7_65572837_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Tfap2c|Gm22773_chr2_172774531_F TCGTCGGCAGCGTCATCTTCCTGAGGCCTGTCCC 60.9 0.00
mm4_intergenic_Tfap2c|Gm22773_chr2_172774531_R GTCTCGTGGGCTCGGTAGGACAGGAGCTCCCACTC 60.0 0.00
mm4_intergenic_Prima1|Fam181a_chr12_103242695_F TCGTCGGCAGCGTCGGAAAACAGACGTGAGCGGA 60.5 0.00
mm4_intergenic_Prima1|Fam181a_chr12_103242695_R GTCTCGTGGGCTCGGAGACGAGGAATCACTGCAGC 60.1 0.00
mm4_intergenic_Pdgfra|Gm22084_chr5_75250480_F TCGTCGGCAGCGTCTCCTCACGACAGTCCTGGAG 60.6 0.00
mm4_intergenic_Pdgfra|Gm22084_chr5_75250480_R GTCTCGTGGGCTCGGCCATTCTGTGTCACCCTGTGA 59.9 0.00
mm4_intergenic_Nup54|Gm26039_chr5_92429323_F TCGTCGGCAGCGTCCTCAGTGATCAGGTGCTGCT 59.7 0.00
mm4_intergenic_Nup54|Gm26039_chr5_92429323_R GTCTCGTGGGCTCGGTGCAGCCTGGTGAATTGGTT 60.4 0.00
mm4_intergenic_Nckipsd|Celsr3_chr9_108826277_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Nckipsd|Celsr3_chr9_108826277_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm5530|mmu-mir-6344_chr1_82071213_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm5530|mmu-mir-6344_chr1_82071213_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25758|Adamts3_chr5_89829147_F TCGTCGGCAGCGTCAGCAAGCCTGAGATGACCAC 60.0 0.00
mm4_intergenic_Gm25758|Adamts3_chr5_89829147_R GTCTCGTGGGCTCGGCACTGGGCAGAGGTCATCTC 59.8 0.00
mm4_intergenic_Gm25454|n-R5s165_chr6_112208713_F TCGTCGGCAGCGTCTCCCACTGGATAGCATGACTC 58.9 0.00
mm4_intergenic_Gm25454|n-R5s165_chr6_112208713_R GTCTCGTGGGCTCGGCTTGAGCCTTGCAAGCTGC 60.0 0.00
mm4_intergenic_Gm23752|Gm24815_chr12_7171842_F TCGTCGGCAGCGTCACGGAATGCTCCAGTCCTTG 60.0 0.00
mm4_intergenic_Gm23752|Gm24815_chr12_7171842_R GTCTCGTGGGCTCGGTGGGAGATGGTGAGAGACAGA 59.6 0.00
mm4_intergenic_Gm22346|Mcfd2_chr17_87246745_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm22346|Mcfd2_chr17_87246745_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm22227|Zfp703_chr8_26354046_F TCGTCGGCAGCGTCTTGGTGGATGGGGAGCAAAA 59.8 0.00
mm4_intergenic_Gm22227|Zfp703_chr8_26354046_R GTCTCGTGGGCTCGGACCTTCCTGGCTCTTACCCT 59.8 0.00
mm4_intergenic_Fignl2|Ankrd33_chr15_101066399_F TCGTCGGCAGCGTCGAGATGGCCTCAGCTTTGCT 60.3 0.00
mm4_intergenic_Fignl2|Ankrd33_chr15_101066399_R GTCTCGTGGGCTCGGCTGCCCTGTCCTACCTTGTG 60.0 0.00
mm4_intergenic_Chst14|Gm14089_chr2_118940063_F TCGTCGGCAGCGTCGGCCTGAGAGACACACAGTG 60.3 0.00
mm4_intergenic_Chst14|Gm14089_chr2_118940063_R GTCTCGTGGGCTCGGGCATCTTCCCCTGTCTGCTT 60.0 0.00
mm4_intergenic_Chid1|Ap2a2_chr7_141544951_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Chid1|Ap2a2_chr7_141544951_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_1700041M05Rik|Grik3_chr4_125451107_F TCGTCGGCAGCGTCTAGTTTGCCGGGAGAAGCTG 60.0 0.00
mm4_intergenic_1700041M05Rik|Grik3_chr4_125451107_R GTCTCGTGGGCTCGGTGGAGACAGAAGCCCAACTC 59.3 0.00
mm4_exon_Samd4b_chr7_28400909_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Samd4b_chr7_28400909_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Rbm47_chr5_66019237_F TCGTCGGCAGCGTCCTGGTAGACGTCGTGGATGG 59.8 0.00
mm4_exon_Rbm47_chr5_66019237_R GTCTCGTGGGCTCGGCTACGCCGGCTACATACCTC 59.7 0.00
mm4_exon_Rarg_chr15_102246295_F TCGTCGGCAGCGTCCTAGAACACCAAGGACCCGC 60.3 0.00
mm4_exon_Rarg_chr15_102246295_R GTCTCGTGGGCTCGGCATGCATCTCAGCGCCCTA 59.9 0.00
mm4_exon_Plekhg2_chr7_28367735_F TCGTCGGCAGCGTCAACAAGAAGAGGAGGCGCTC 60.0 0.00
mm4_exon_Plekhg2_chr7_28367735_R GTCTCGTGGGCTCGGGCGCCTTTGGAGAGCTAGTA 59.5 0.00
mm4_exon_Pde2a_chr7_101509912_F TCGTCGGCAGCGTCATGTGTGCAACAGGGACCAT 59.8 0.00
mm4_exon_Pde2a_chr7_101509912_R GTCTCGTGGGCTCGGCTTGAAGATGCGGAGGTGGT 60.0 0.00
mm4_exon_Msi1_chr5_115447321_F TCGTCGGCAGCGTCAGAAAGGAGGGGAGAGGGAC 59.9 0.00
mm4_exon_Msi1_chr5_115447321_R GTCTCGTGGGCTCGGCTTTACCTCCGTCTGCCTGG 60.1 0.00
mm4_exon_Mrc2_chr11_105292878_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Mrc2_chr11_105292878_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Gpc1_chr1_92831649_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Gpc1_chr1_92831649_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_C77080_chr4_129223507_F TCGTCGGCAGCGTCCTGCTTTCGCTCAGGTTTGG 59.7 0.00
mm4_exon_C77080_chr4_129223507_R GTCTCGTGGGCTCGGCTGTCTGGCAGGAGTGTGTC 60.3 0.00
mm3_intron_Tmem136_chr9_43116245_F TCGTCGGCAGCGTCCCAGCAGCAAGGTCGCAT 60.7 0.00
mm3_intron_Tmem136_chr9_43116245_R GTCTCGTGGGCTCGGACCCGTCTGGATGCCTAGAT 60.1 0.00
mm3_intron_Exoc4_chr6_33249348_F TCGTCGGCAGCGTCGTGGAAGGGAGAGGCATCG 59.8 0.00
mm3_intron_Exoc4_chr6_33249348_R GTCTCGTGGGCTCGGCGGCCCCAGAGAGTCTTAGA 60.3 0.00
mm3_intron_Dlg5_chr14_24144672_F TCGTCGGCAGCGTCTCAGAGAGCTCAATGCAGCA 59.3 0.00
mm3_intron_Dlg5_chr14_24144672_R GTCTCGTGGGCTCGGAGGAAGCACAGCTAGCAGTG 60.0 0.00
mm3_exon_Krtap1-4_chr11_99583291_F Primer3: not found at this Tm N.d. 0.00
mm3_exon_Krtap1-4_chr11_99583291_R Primer3: not found at this Tm N.d. 0.00
mm2_intron_Fblim1_chr4_141599205_F TCGTCGGCAGCGTCTCCTCAGGCTACCGGTCTTT 60.2 0.00
mm2_intron_Fblim1_chr4_141599205_R GTCTCGTGGGCTCGGCTGGGGCTGAACGACCAG 60.0 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314014 AACCGCCAGAGAAGATGGTGCCCCACGGTTGCCTGAGCCCGCGAGCCGGCCCTCCGACTTCCCGGGAGCGTGGCGGGGGA
GGCC
AGGAGGAGGAGCCGGTCGATGGACTAGCAGGCAG
mm3_exon_Obscn_chr11_59135720 TATGCCCTCTGCGCGCATGGCCTCCCGCCGCTGCTGCAGGTGCGCCAGGAGCGTGGCGGTGGAGGTGCCGGGTGGTCCGG
CAACCTCGGCGTCCGAGTCCACCACCAGAG
mm3_intergenic_Gm11523|Cdk5rap3_chr11_96886125 TTCGCTTCAGCCTCACAAGGCCACGGGTGGCCTCCCCCCACACACTCCAGGCCCCTCTTGGGGGCCCTTTGGGGAAGGGG
CTCTGCTCCACTCACCAGCAGCCTGGCAGGCAGGGCAGCTTGGTAACTGCAGGCAG
mm4_intron_Shc3_chr13_51469116 GAAGTGGTTTGTGCTGGCTGGCCCGTACTGTTTCATCCGGAAGAAAATATCCTCTCCCCTCCAAACAGATCCAGAAAATC
CTGCCAGGAGTGTGGAGGGGGAGGGATGGGTGGGCGTGGCACTCTCCAAGCCTTCAGA
mm4_exon_Tsc22d2_chr3_58416019 CCGAACCTCATCCTGGATGGGCAGCTGGCAGCCGCTTCAGCTGCGCCCGCCAACGGAGGCGGCGGTGGCGTGTCGGCCCG
GAGCGTGGCGGGGGCACTC
GCCCAGACCCTGGCGGCGGCCGCCGCTTCGGTGTCCACTCC
mm4_exon_Zbtb7a_chr10_81144245 TGGACCAGCAGAACGTGTACGAGATCGACTTCGTGAGTGCCGAGGCACTGACGGCGCTCATGGACTTCGCCTACACCGCC
ACGCTCACGG
TCAGCACGGCCAATGTGGGCGACATCCTGAG
mm3_exon_Hmcn1_chr1_150993403 GCAGGGAGCTGCGAGTAGCCGAGCGCCCGCAGAGCGCGGGGTCCATGCCCTGGGGGACGCTGCGAGCCTGCGGTGCCGCC
TCCGCCGCCGCGCTCCAGG
CCAGCGACTGAGCCTCCCAACTTT
mm4_exon_Ltb_chr17_35196232 TGTGAATCGTGATGTCCGGGACCCCGACAGTCCCGANNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAACGGAATGTAGGA
CACGAATTTTGAAAATAAAGAATGTAAACTATGCCGGCCCTTGCCAGTGTCTTCACGGAA
mm4_intron_Crtc1_chr8_70420527 GGTCAAAGCCACAGCAAAGGCAGATAGAGAATGGAGCCCTGTGCTGCACACCACACATCTGTGACCTAATCTCGTTATGC
TGTGGGTTCAGCAAAGGCCCAGAGCGTGACAGGGGAGGCAGACAGTCGCAGGAAAGAGGC
mm4_exon_Aldh1a2_chr9_71215884 CCCATGACTTCCAGCGAGATCGCCATGCCGGGCGAGGTGAAGGCCGACCCCGCCGCGCTCATGGCCTCGCTGCAGCTCCT
GCCGTCGCCCACGCCCAACCTCGAGATCAAGT
mm4_intron_Tprg_chr16_25368085 GCTCCAGCAACATCTCCTGTCTAGACAATGTAATTATGAGCACAGAGCAAATAGCTGAAGTGTATGCCGCCCCCAAGGGT
TGCATAACTCCAGGAGCGGGGCTGGGGAGACAGAGGGAGGTGTGGATGCTGTTA
mm4_exon_Phf15_chr11_51857442 GCTCCCGAAGCCAGGAGCTCCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCCATCCGGGGTCGTGGCGAGGGAGGC
G
ACCGGGCCGCGGCGCCCCCCAAGCCCCGCACCCCTGCTGGGAAGATGGTCAG
mm4_intergenic_Gm25199|Syt6_chr3_103575160 GAACCTGCCCGGACTCAGGCAAGTCCCTGAGTGTGGAGGGGGCGGCCGTGAGGCGCCAGACTTGCCAGCTGCCGCGGGAT
GCCACGTGAGGGGCGGGGACCCTGCGGGGAAGTGATTAAAAGCAGAGCGGAGCCTC
mm4_exon_Cnnm1_chr19_43440895 GACGTGGAGGTCCTGGGGCCCCTGCGCCCCGGGGGCGTGGCGGGCTCGGCCCTGGTCCAGGTGCGGGTG
mm4_intergenic_Actrt2|Gm13113_chr4_154701181 CTTCCCAGAGAGACCGCAAGCGAAGAAGTCGAGGACTCGAGACACCACCCCCTCCACGCTCCCGGCTGCTCCTTGGTGTG
TGAGCGAT
mm4_intergenic_Zg16|Gm22747_chr7_127055684 CCCCAGGCAACACTTCATTTCATCAAACATTACAAGTGAGGCAAGATAGGAAGACAGAGAATTAAGGGGACAAGAGAAAG
GAAGAGAGCCCCCCCCCCCACGCTCTTGGATCTCGTTTAAATTTCCCATTGGT
mm4_exon_Gm10760/Mctp1_chr13_76384444 CTCCGAGCTGGCCTGAAGCCGCTGGACGCTCCGGGCTTGTGCTGCTGCGCTGNNNNNNNNNNNNNNNNNNNNNNNNTCCA
CGCTCCCGG
AGGAGGCGCAGCCCGAGAGGGAGGAGGAAAGGCAA
mm4_intergenic_Zic1|Zic4/Zic1_chr9_91361962 AATGGGACTTGGGCTTGGTTCTCCCGGCGTAGCCCAGCACCGCCACGCTCCAGGAGGGGACTCTGCCCTACAGTACTCAC
AAAAGAAAAAGAGGAGGGGGGCTGGGAAGGAGGAGGCTGCTTTT
mm4_exon_Rd3/Gm26670_chr1_191985444 ACCATCTCGGAGGATGTGGAGCGCGATGCGCCACCCCCGCCGCGCACCTGGAGCATGCCGGAGTTCAGGGCGCCCCAGGC
CGACTGACCACCCAGGGCAATCACCCTCAGGAGGAATAACGGGCTGG
mm4_intergenic_Gm14513|Gm26314_chrX_11714054 GGGAGCATTTGTTGAAGCGAGGACAAATCAAGTACCTAGTAGCATTTCATCAACAGTGATGAAGAGGATCAGTAGACTAA
GCAACCTGGAGCTAGGCATGGGAGGCCCCTGAAACACAGAAAGGCCC
mm4_intron_Sez6_chr11_77931462 TCCACTCCAGGCGTCTAGGGGCGCCCGGGCGCGTTGCGGGGGGGGCGGCAGGTCGCGGGTGCAGAGTCCGGACAGCCCAG
CTACCAGCCCGCCGTTCTGCCTACCCCGCTGGTCTCCAAACTTCGCTCGA
mm4_intergenic_Cops8|Gm9991_chr1_90644089 GTCTTGCGCAGTCCAATGTGAGCAGCGTGGGTCTCCCGTGCCAAGCTCCTGGTTTTATAGAGTCTACAAACAATTAATGA
GAGCCTTGGCTGGATTCGTGAGGGAGTATGCAATCCAGGGG
mm4_intron_Rxra_chr2_27677639 GTGGCGGTACCGAGAGTTAGCGCGCCGGGCCCGGGGAGGCGAGTGGGCACAGGGCAACTTTTCCCGGGCCCAGGCGCGCG
GCGGGAGAGGGC
GAGGACGCCGGGCCTCTGACTTGCCGAGGAGC
mm4_intergenic_Smyd2|Prox1_chr1_189922419 GAACTCGGCGCTCTGCTCGGGGCGGGTGCTGTGCCCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNACGGGAGTCCGCCTGCACCCGCCAGGCTGCGCTGCCCGGTGAGGAAGGCTGGAG
mm4_intron_Arhgef37_chr18_61499466 GGTTTGCCTTGCTTGCACAGCACCCAGGAACGGGGCGAGGAAGGCCACATTGGATCTTACTTTAGACATTTCATGAACCA
CTGAATTACTGTAAGCTGACTGGCACCA
mm4_exon_Gm7701_chr18_24258301 GGTTGTTCAGGCCCATGAGAGTGAGGTGACAGGCCTCAGCCTCCACGCTACTGGAGACTATCTCCTGAGAGCCTCTGATG
ATCAGTACTGGGCCTTCTCTGACGTGACAGATGAGACCTCCG
mm3_intron_Jarid2_chr13_44730342 CTCCAACCCCTCGCTGACANNNNNNNNNNNNNNNNNNNNNNNNACGCTCCGAGGCCTTTCCCCCGGGAAGCCTGGCGGCT
TGCCCTCGCGGGCCCGCGGGCGCGCTCTCGCGCGCCGATTCCTCGATTCCACAGGCG
mm4_intron_Zc3h8_chr2_128943786 CTGACTTCCACCCCACTTCCCCTTCCGACCTCCCCGCTCCGGTCCCGGCCCACGCGCCAGCCAGGGCTCGCCGGTTTCCA
GGAGCGAGGGGGCGGAGCCC
AAGCGGTGCCCTGCAAAGGACCAGAACGTGGGGATCAC
mm4_exon_Ndnl2_chr7_64872848 GACGTGCTGGGTCCTTCCTCGCCAGCGCCTCCCCAGTCCCGCTCCGGGTCAGCCTGCGTGCTGGGCCGACCGCGGCCCCT
CGGCTTCTGCAACATGTC
mm4_intron_Gm11762_chr11_119565558 ATGAAGCTGCTGTCCCTCACCCGCAGCCCCCAGACCCCTTGCCCTCCCCCGGCTCGCCCCAGGTTCTGGCTGTAGATTGN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCACAGAAGCATGAGGTCTGCC
mm4_intron_B230219D22Rik_chr13_55693577 AGTCTCGCCCTGGAGCTGCGGTCGTCCCCGCATCCCGAGAGCGCGGCGGGGAACGCCTCGGGGTCCCGTTAGGATGCTGA
CTTGGGGAAGCGCCGTGGGGCACGGCTAAGGAGCGCAGAGAAGA
mm4_intergenic_Fam49b|Gm25628_chr15_63997974 CTCGTCACGGCGCTAGGGGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCGGGAGCCCCTCCGCACC
TGCCCCTCTGGCGGAGCCGCGAAGAGCCAGGCGACAG
mm4_intergenic_Rhobtb3|Gm6311_chr13_75943948 TCGGAAGGCTGAAACCTGAGCCGGGAGCGGGGGAGGGGCGGCCTGGCCCCCGCCGGGGACCAGAAGAGAGCCCAGCCAGG
AGGCAGCCCCCTGCCCACCCCGCGGTCCCGTACCCACCCCAGCCTTCGCAGAAAGTGTGT
mm4_intron_Nek7_chr1_138618062 GGCCCACATCCTCAGACAAACTTGGCCTCCCTCGCCTAGCTCAGGGCAGCGGTAGGAAGTACCCGCCCTCC
mm4_intron_Ehd2_chr7_15955204 GGGAGGCGTGGCTTGTTATTCAAGGGGCGTGGCCTTCCAGGGAGGCCCAAATGGGAGGACAGAGTCTGCCAGGACCGTGG
TAGGGGAGGTC
CTGCCATGGCTTCCATAGCAGAAAGAGGCAGGCG
mm4_intergenic_Klf14|Mir29a_chr6_30959067 TGGCGAGCCAGGTAATTTCAATGCGCGCAAGTCCCGGATCGCAACTTCTGGGCAGCCTGCCGGCCTCGCCCCCCTCGCCC
CTGG
TCTCTGGCCAGCTGGGTGTCAGGCAGAGTCCTA
mm4_intergenic_Gli2|mmu-mir-6346_chr1_118927093 TGCAGGTTACAGGAGCAAGGCCTGCCGCTGGGCACATCTGTCTTTCCCAAATGCTGACACAGGGAGAAGTCTGCACAACC
TGGGCTCCCCACCCAAGCTCCAGGAACCTTTCTCCTGGTGGGCATT
mm4_intron_Sirpa_chr2_129594922 GCTTCCTCCTGCCTTACCAGTCCCAGCCCTACCCTGCCATGCTCCAGGGCTTGGGAATCTACAGGCATCTCTCCGAAGTA
CAGAACACTGGGCCCAGATT
mm4_intergenic_Tcte2|Gm24867_chr17_13667284 CTGAGAACACAGAGGCTGGGAGGGGCCTCCCCCACCACACACGTGGTATATACACAAGTCTCCAGGAACAGTCTCCCTCT
GTGAGGTAGGTGGGTCCCCTCTCCTCCCCAGACCAGCCTTGATTT
mm4_intergenic_Zeb2/Gm13476/ZEB2_AS1_3/ZEB2_AS1_4|Zeb2_chr2_45117167 GAACTTCGCTCCCTCTGTCGGTCCCGGAGCACGTGGGGGGGGGGGGAATCGGCGGCCCGCACCCCCCTGCAACACCCGAC
CCCGCCCTGGTACCGCGCGACCCCCGCCCCGCTCCCGGTCAGCTGACCTGGCTCGC
mm4_intergenic_Sdccag8|Gm15423_chr1_176927625 TGTGCAATGAACATCGACGCTGGGGGTGCTCTCCTCTCCCCTGCCACACAGGCCCACAGCCTGGAGCATGGCGGCTGAGG
CA
GCCAACTCATCTTCTGAAGAAGCCCAGAGGAAACCAAGAGCGT
mm4_intron_Zfr_chr15_12118808 ATGGAGAGAGAGTGAGCCGAGGGGCCTCGCGCGGAGTGCGGAGTCAGCGGCCTCTACCGCGACGCCCCCGGAGCCCAGAT
CACACAGCAGGTCTTTGTCGGTACC
mm4_exon_D030062O11Rik/Tbx18_chr9_87730405 CAGAGAGCCGCGGATGGACGGTGTGCGGGGCCGACGGCTCAACGCCTGCCCCCGGGGCTCGGCGGCGGAGGCCCGGGAGG
AGCGGGCGCTAGCGCTGCCTCACCGAAGCTCAGGTTC
mm3_exon_Surf4_chr2_26933509 GGCCAAGCCTTAAAGGGACCGCGCCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNGAGG
AGCCTGGCGCCGGCGCACCGCCCTTCACGAACAG
mm4_intron_Grhl3_chr4_135564040 TGAGCCTGGGGTTAGAGGAACTAAGTGAACAGCCCCTCCCCTGCCAGGCTCCAGGCTAGGCAACTCCCTAACTAAGGCGG
CATGGGAAAAGCAAGGGGTGTCTAAGGGAC
mm4_intron_Ryr1_chr7_29090289 AAGACAACCCAACCGTGGTTAGGGCACACATCTGCCTGCAGACAGGGAACCCTCAAGGCCTCCTTCCCCATGCTCCAGGA
ATACTGTGACTTCAGAGCCCCACACACAAACACCCACCAC
mm4_intergenic_Psma7|Ss18l1_chr2_180042436 CTCAAAAGCGCACACTCACCGTCAGCGCCCCGCCCTCCCGGGCCACGCTGCTGGCGTCTGGCGTCAGGGGGACGTGCGGC
ACGGCGGGGCGGGGCAGGGCGGGCGGAACCACCGAAGCTCAGCA
mm4_intron_Nrg2_chr18_36196031 ACTTGTAAGCGGAGGCCTTCACAGCCTGGGAGTCTGCCCAGCTGCTCCTGTAGACCCTGCCAGCCTGGGCAAGGCAGGCC
CCCGGGAGCTGAGCGGGCGAGGCCTCCGAGGGTCACTCCTGACTCACTGAGCCTCA
mm4_intron_Ankfn1_chr11_89538111 GCTCTTCGTGTGACTGAGCTTGCTGTAAGGAGAATGCCCACTTGTCAGAAATGGGCCTTCCCCACCCTGCTCCTGGCTGT
TCCACAGGGCAGTGTGTGCACTATCTCAGACGGC
mm4_intron_Celsr1_chr15_86029994 CCCCACAAACCTGCTGAAGAAGGCAATGAGGGGGCCTCCTCCTCCACGGACCTGGGTAGGGAGCACGCCCCCACCATGCA
ACCAATCACAGCAGCAA
mm4_exon_Gm12462/Pax5_chr4_44684618 TTCTCTTTCCCCGAAGCCTGGCAGCTCTGCCGCTCTCCAGGAGCATGGGGAGGGAGCCCTGATGGGGAGCTTCCAGCAGG
ATCAGTGGGGGCCTCAGACACCACTAGCATGGCAC
mm4_intergenic_Gm24338|Ppp2r3a_chr9_101251414 TCCTGTTAACGAGTTCCCGGCCCGGGCGTCCCGCGAGGTGGGAGGCGCCGGCCATCCGGCCCCCTGGCCTCCTCCGCGGA
GCTCCCGG
GCCACCGTTCTCCACGGCCGCCAGCCCCGGGAGTCCTCTGGCGTGG
mm4_exon_Tbkbp1_chr11_97138893 GTAGAGCTCGCCGCCATAGGCGCGGGGCTTGGGAAGTGTGGCAGCCTCGGCCTGTAGCCAGGCGGGGGAGGCACCAGCGT
AGGCCGCCCCCTCCGCCAGCTCCGAGTAGCTG
mm4_intergenic_C130040N14Rik/AI413582|Nudt3_chr17_27574903 CTTGCAGAGCCAGAGAGGACTGCCAAGGCCTCCCCAGCAGGGCTCCGGGAAGGGGGCCAGGCTGGGCTGGCTGCAAGAGC
GAGTGAGAGGAA
mm4_intergenic_Atp1a1|Mab21l3_chr3_101797617 GGTCCAAAGAGGGTGGTAGGATATGGAAATCTGTGGCCACACCCTCCAGGCTCCAGGGATACTGGCCCTTTTCTCTGTTC
AACACGAATGCTCTCCAACAAACAGCATGGTATCCAGCTTCATCCTCATGCAGCCTGG
mm4_intron_Fendrr_chr8_121070362 TGGAAGCTCTTCTGCTCAGCCCAGGCTGGAACCTGGGGCCTGGCCTGGGAGGCCAAGTCCAGCCCTCTCCCCTGGTCCCC
AGTTCTACTCTGCCCCTCGATGCTGGCTCTGCTTATTCATTCCTCCCAGCAACCC
mm4_exon_Apbb1_chr7_105574105 GCATTAAGCCTTTGGGACCCAAAGGTGCCGTCTCAGCCTCCTGGCTGGCCTCCTCCGCCAAGGTCAAGGTCACATTTCGG
TTCTGGTCTCGGTG
mm3_exon_Clk3_chr9_57765258 CGAACTCGCAGACTCTGGGGGACTGGCCTGCCCCGCCAGGGTCCGGGCGACATCGGTGGAGGTCTCTTCC
mm4_intergenic_Gm13002|Wnt4_chr4_137277310 CTCTAGCTCGGCCCAACTGGGCGGCGTGCGGCCAAGCGTGCCTGCAGCAGCGGGGCTGAGGCTCCGAGCGCGTCCGGGCG
CCGGGCCGGGGAGGCC
CCCCAGGAGAAGCCCAGT
mm4_exon_Nup153_chr13_46727537 CCCCTCCTATGCCTCCGGCTTCCGAGGCCATGGCGGAGCCTGGGCGGCCTCCCACTACCGCTCCCGGGCGGGAGAGGCAG
AGGCGGCGGCCTGAGGGCCTCCCCCGCCCGGCCCTAGGCGACACACAGACACAGCGAGA
mm4_intron_Plekhm3_chr1_64845276 GACCCGTTGTTCCTGTCCTTACCCAGGAAAAGGCTGAGGGACCTCCAGCCTGGCGGAGGAGGCCTTTGCTTCTCTAGCAA
GAGGATCTTGGTGGCAGTTTGC
mm4_intron_Nphp4_chr4_152556601 GCAGCCTGGATCCTTGTCTTCCTGGTGGGTGTTGGGGGAGGCCGCACCTGCTGCCATCCCTGTTGTTGGCTGACCCCTTC
CCCTGCAGCATGGGCTCCTCTGCACAGAGGCAGCAGGGAAGGCATAGGTC
mm4_intron_Kcnn2_chr18_45675601 CCAACTCTCAGATGGCACCAATTTCTCTACCTTTGAAAAGACCCAAGCTTTGGCTTCTTTCTAGTCTTAACTTCCCTGGA
GAGAGGGAGGGGAGGCC
GCTACTTTGACTCACTGCTGTATTCTGCATCTCTTCCTGCTGT
mm4_intron_Grik4_chr9_42733745 CCACTGTTGGCTGAGCATAAGCTCCTCCCCCTCCACTCTCCAGGCCAGTTTCTGGGTTCCCTCCTTGTGCGCATTGAGAT
CCATGGGCTCAGAGC
mm4_intron_Fbxo21_chr5_117977099 GAGGTGAGCGAGCATCCCGTTCCCGGGACCCGCAGAGCCCCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNN
NNNNNNNNNNNNNNNNNNNNNTCTTCCCGCGGCCGCAGACAAAGGCCTGCCTGC
mm4_intron_Fam129b_chr2_32881258 CTCCCACCCTTTAGCCTGTGCTCTGGCCTCCTGGGGGCCTCCCACCTCACCCTCCAGGCCTTCCCACACAGGCTGCCAAA
AGAGA
mm4_intergenic_Tfap2c|Gm22773_chr2_172774531 ATCTTCCTGAGGCCTGTCCCGGGGCTCAGGGCCCTGCTGAGCTGGGCGAAGAGCTCTGGGCCTCCACAGCCACCATCCTG
G
TTTCTATAACCAGACTTCTATAGCTAGCACTCTGGAGTGGGAGCTCCTGTCCTA
mm4_intergenic_Prima1|Fam181a_chr12_103242695 GGAAAACAGACGTGAGCGGAGCCAGAGCCTCGCCCGCCTCCCTCCCGGGCTGCACTCCCGCCCGCGCTCCCCGCTGCCGC
GGCCGCCAGCGCCGCTGCAGTGATTCCTCGTCT
mm4_intergenic_Pdgfra|Gm22084_chr5_75250480 TCCTCACGACAGTCCTGGAGGGTGAGCAGCACAGGTGATGTTCCTGTGGTTGAGGCCTGGAGACAGGCTGGGGAGGCCAA
GTCACAGGGTGACACAGAATGG
mm4_intergenic_Nup54|Gm26039_chr5_92429323 CTCAGTGATCAGGTGCTGCTACAGACCCGAGGACACAAGCACGGGCAGCAGCACACACTGGGCCCATGGTGCCGGGAGGG
GGGCTGGGGAGCCC
CAGGAACCAGCTGGCCAGTGGTCTAACCAATTCACCAGGCTGCA
mm4_intergenic_Gm25758|Adamts3_chr5_89829147 AGCAAGCCTGAGATGACCACCAGTCAGGTGTCATGTATGCCTGGAGGTTGGGGGGGCAGGCCTGAGATGACCTCTGCCCA
GTG
mm4_intergenic_Gm25454|n-R5s165_chr6_112208713 TCCCACTGGATAGCATGACTCTGTGGTGTAGTTATCTCTTCTAAATTGAAACATTTCTGCCTAGAGGGTGATGGGGGAGG
CC
CATGAGACGTGATGACCTAATGCAGCTTGCAAGGCTCAAG
mm4_intergenic_Gm23752|Gm24815_chr12_7171842 ACGGAATGCTCCAGTCCTTGACCAATGCTCTGTATTGAAACTCCAGCCTCTCCCCCCTCCCCCCCCACCCTCCAGGGTCT
CTTTGCCTAACTCTGTCTCTCACCATCTCCCA
mm4_intergenic_Gm22227|Zfp703_chr8_26354046 TTGGTGGATGGGGAGCAAAACTGAGGCCTCCCCCCCCCCCCCCCGGGGTGGGAGGGTAAGAGCCAGGAAGGT
mm4_intergenic_Fignl2|Ankrd33_chr15_101066399 GAGATGGCCTCAGCTTTGCTGGGGCAACCCCCTCCCCCAGCCCAGCCTGTCTGGTCTGCCCTGCCCGGAGAGAGGAGGGG
CAGGCC
GGAGAGCAGGACACAAGGTAGGACAGGGCAG
mm4_intergenic_Chst14|Gm14089_chr2_118940063 GGCCTGAGAGACACACAGTGCTATGGAATGTGGGCTTCTCCTGGGGAGTGGGGGGGGAGGTCTGAGAACATAGAGGAGGC
AGCACAGCATCCCTGCAGTGAAGCAGACAGGGGAAGATGC
mm4_intergenic_1700041M05Rik|Grik3_chr4_125451107 TAGTTTGCCGGGAGAAGCTGCATAAATCAGGATTTCAAACAAAGGCTCTCCATCCAGGCCAGGGGCTCCCTAGTCACTCC
CAGGAGAGAGGCTGGGGAAGCC
CAGAAGCAGTGCATGTAGAGTTGGGCTTCTGTCTCCA
mm4_exon_Rbm47_chr5_66019237 CTGGTAGACGTCGTGGATGGGCACCTGGAGAGCAGCGGGGAAGGCCTGAGGTATGTAGCCGGCGTAG
mm4_exon_Rarg_chr15_102246295 CTAGAACACCAAGGACCCGCAGCCCATTCCCCCTCCTCCCCCCGCAGCGCCCCGCTCCTGGAGGGGGGAGGGGGAGGGCT
AGCGTAGGGCGCTGAGATGCATG
mm4_exon_Plekhg2_chr7_28367735 AACAAGAAGAGGAGGCGCTCACCCCCACGAAGCCTGGGGCCTCCCCCGCCTCCCCCTCGGAAAGTGCCTTCCAGTACTAG
CTCTCCAAAGGCGC
mm4_exon_Pde2a_chr7_101509912 ATGTGTGCAACAGGGACCATGACAGCCCTCCCCTGACACTCTCCAGGACTATCAGCGCATGCTGGACCTGATGAGGGACA
TCATCTTGGCTACAGACCTGGCACACCACCTCCGCATCTTCAAG
mm4_exon_Msi1_chr5_115447321 AGAAAGGAGGGGAGAGGGACTGTCCCCTTCCCCGGGGTGAAGCTGGCAGAACGACTGTACCTGGGCACTGGCCAGTTTCT
TCAGCTACATGGCCCTCGAGGGTGGGGCGGGAGGCCAAGCCAGGCAGACGGAGGTAAAG
mm4_exon_C77080_chr4_129223507 CTGCTTTCGCTCAGGTTTGGGCGGCAACCCTAAGGGCCCATCAGGCACTGCGGAGCCGCGCTGATGGAGGGAGTAGGTCC
GGCGGGGAGGCGGGGGAGGCC
GTTTCATCTTACGCAGGGACACACTCCTGCCAGACAG
mm3_intron_Tmem136_chr9_43116245 CCAGCAGCAAGGTCGCATCCCGGAGGGTGGCGGGGGAGTGCCCAACTGCTCGACCACGCCCGACTTCTAACCCGGAACGT
CCCATCCTGAACCGTTATCTAGGCATCCAGACGGGT
mm3_intron_Exoc4_chr6_33249348 GTGGAAGGGAGAGGCATCGGCTGGGCCCCATCCGCCGGCCCTTCTCTCCCTTTTGGCCTCCCCCGCCCCTCCCCAGGGCC
TATTGCCCGCCCCCGCCGGGCAGTGTTCTAAGACTCTCTGGGGCCG
mm3_intron_Dlg5_chr14_24144672 TCAGAGAGCTCAATGCAGCAGCCGGGAGGGGGGCGGGGGGGGCCAGTGACACTGCTAGCTGTGCTTCCT
mm2_intron_Fblim1_chr4_141599205 TCCTCAGGCTACCGGTCTTTTGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGAGGCCAGCTGGTCGTTCAGCCCCAG

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_mN1FkRligAThyqKv3eg3.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.