CRISPOR

DNA break repair outcome predictions

Guide: GAAGTCGGAGGGCCGGCTCG CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CTCCCCCGCCACGCTCCCGGGAAGTCGGAGGGCCGGCTCGCGGGCTCAGGCAACCGTGGGGCACCATCTTCTCTGGCGGT`	(Wild-type sequence)
603	`ACGCTCCCGGGAAGTCGGAGGGCCGGCTC--------AGGCAACCGTGGGGCACCATCTT`	`8 bp deleted → frameshift`
439	`ACGCTCCCGGGAAGTCGGAGGGC------------TCAGGCAACCGTGGGGCACCATCTT`	`12 bp deleted → no frameshift`
423	`ACGCTCCCGGGAAGTCGGAGGGCCGG-------GCTCAGGCAACCGTGGGGCACCATCTT`	`7 bp deleted → frameshift`
362	`ACGCTCCCGGGAAGTCGGAGGGCCGGCTCG--GGCTCAGGCAACCGTGGGGCACCATCTT`	`2 bp deleted → frameshift`
298	`ACGCTCCCGGGAAGTCGGAGGGCCGGC--------------AACCGTGGGGCACCATCTT`	`14 bp deleted → frameshift`
283	`ACGCTCCCGGGAAGTCGG---------------GCTCAGGCAACCGTGGGGCACCATCTT`	`15 bp deleted → no frameshift`
253	`ACGCTCCCGGG-----------------------CTCAGGCAACCGTGGGGCACCATCTT`	`23 bp deleted → frameshift`
244	`ACGCTCCCGGGAAGTCGGAGGGC------------------AACCGTGGGGCACCATCTT`	`18 bp deleted → no frameshift`
230	`ACGCTCCCGGGAAGTCGGAGGG-----------GCTCAGGCAACCGTGGGGCACCATCTT`	`11 bp deleted → frameshift`
210	`ACGCTCCCGGGAAGTCGGAGGGCCG---------------------TGGGGCACCATCTT`	`21 bp deleted → no frameshift`
208	`ACGCTCCCGGGAAGTCGGAGG-------------CTCAGGCAACCGTGGGGCACCATCTT`	`13 bp deleted → frameshift`
193	`ACGCTCCCGGGAAGTCGGAGG-------------------CAACCGTGGGGCACCATCTT`	`19 bp deleted → frameshift`
188	`ACGCTCCCGGGAAGTCGGAGGGC-----------------------------ACCATCTT`	`29 bp deleted → frameshift`
179	`ACGCTCCCGGGAAGTCGGAGGGCCGGCTCG----------------TGGGGCACCATCTT`	`16 bp deleted → frameshift`
179	`ACGCTCCCGGGAAGTCGG----------------CTCAGGCAACCGTGGGGCACCATCTT`	`16 bp deleted → frameshift`
172	`ACGCTCCCGGGAAGTCGGAGGGCCGGC-------------------------ACCATCTT`	`25 bp deleted → frameshift`
148	`ACGCTC-------------------------------AGGCAACCGTGGGGCACCATCTT`	`31 bp deleted → frameshift`
148	`ACGCTCCCGGGAAGTCGGAGGG----------------------------GCACCATCTT`	`28 bp deleted → frameshift`
133	`ACGCTCCCGGGAAGTCGG----------------------CAACCGTGGGGCACCATCTT`	`22 bp deleted → frameshift`
133	`ACGCTCCCGGG----------------------GCTCAGGCAACCGTGGGGCACCATCTT`	`22 bp deleted → frameshift`
126	`ACGCTCCCGGGAAGTCGGAGGGCCGG-----------------------GGCACCATCTT`	`23 bp deleted → frameshift`
120	`ACGCTCCCGGGAAGTCGGAGGGCCGG------------------------GCACCATCTT`	`24 bp deleted → no frameshift`
120	`ACGCTCCCGG------------------------CTCAGGCAACCGTGGGGCACCATCTT`	`24 bp deleted → no frameshift`
105	`ACGCTCCCGGGAAGTC---------------------AGGCAACCGTGGGGCACCATCTT`	`21 bp deleted → no frameshift`
103	`ACGCTCCCGGGAAGTCGGAGGG---------------------------GGCACCATCTT`	`27 bp deleted → no frameshift`
94	`ACGCTCCCG-------------------------------------TGGGGCACCATCTT`	`37 bp deleted → frameshift`
94	`ACGCTCCCGGGAAGTCG-----------------------------TGGGGCACCATCTT`	`29 bp deleted → frameshift`
94	`ACGCTCCCGGG-----------------------------CAACCGTGGGGCACCATCTT`	`29 bp deleted → frameshift`
94	`ACG-----------------------------GGCTCAGGCAACCGTGGGGCACCATCTT`	`29 bp deleted → frameshift`
89	`ACGCTCCCGGGAAGTCGGAGG------------------------------CACCATCTT`	`30 bp deleted → no frameshift`
89	`ACGCTCCCGG------------------------------CAACCGTGGGGCACCATCTT`	`30 bp deleted → no frameshift`
86	`ACGCTCCCGGGAAG-------------------------GCAACCGTGGGGCACCATCTT`	`25 bp deleted → frameshift`
85	`ACGCTCCCGGG---------------------------------------GCACCATCTT`	`39 bp deleted → no frameshift`
84	`ACGCTCCCGGGAAGTCGGAGGGCC-------------------------------ATCTT`	`31 bp deleted → frameshift`
84	`ACGCTCCCGGGAAGTCGG-------------------------------GGCACCATCTT`	`31 bp deleted → frameshift`
81	`ACGCTCCCGGG----------------------------------------CACCATCTT`	`40 bp deleted → frameshift`
80	`ACGCTCCCGGGAAGTCGG--------------------------------GCACCATCTT`	`32 bp deleted → frameshift`
76	`ACGCTCCCGGGAAGTCGG---------------------------------CACCATCTT`	`33 bp deleted → no frameshift`
70	`ACGCTCCCGGGAAGTCGGAGGGCCGGCTC-----------------------------TT`	`29 bp deleted → frameshift`
63	`ACGCTCCCGGGAAGTCGGAGGGCCGGCT-------------------------------T`	`31 bp deleted → frameshift`
62	`ACGC-------------------------------------AACCGTGGGGCACCATCTT`	`37 bp deleted → frameshift`
60	`ACGCTCCCGGGAAGT--------------------------------GGGGCACCATCTT`	`32 bp deleted → frameshift`
60	`ACGCTCCCGGG--------------------------------------GGCACCATCTT`	`38 bp deleted → frameshift`
60	`ACGCTCC--------------------------------------GTGGGGCACCATCTT`	`38 bp deleted → frameshift`
51	`ACGCTCCCGG-----------------------------------------CACCATCTT`	`41 bp deleted → frameshift`
46	`ACG-------------------------------------------TGGGGCACCATCTT`	`43 bp deleted → frameshift`
44	`ACGCTCCCGGGAA------------------------------CCGTGGGGCACCATCTT`	`30 bp deleted → no frameshift`
38	`ACGCTCCC-----------------------------------------------ATCTT`	`47 bp deleted → frameshift`
36	`ACGCTCCCGGGAAGTC------------------------------------------TT`	`42 bp deleted → no frameshift`
36	`AC------------------------------------------CGTGGGGCACCATCTT`	`42 bp deleted → no frameshift`
36	`ACGCTCC------------------------------------------------ATCTT`	`48 bp deleted → no frameshift`
36	`ACGC------------------------------------------------ACCATCTT`	`48 bp deleted → no frameshift`
22	`ACGCTC----------------------------------------------------TT`	`52 bp deleted → frameshift`
22	`AC----------------------------------------------------CATCTT`	`52 bp deleted → frameshift`
20	`ACGCT------------------------------------------------------T`	`54 bp deleted → no frameshift`

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