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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313826_F | Primer3: not found at this Tm | N.d. | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313826_R | Primer3: not found at this Tm | N.d. | 1.00 |
| mm4_intergenic_Pcgf5|Gm23170_chr19_36381832_F | TCGTCGGCAGCGTCCTTGAGAACCTGGCCCTGTT | 59.8 | 0.60 |
| mm4_intergenic_Pcgf5|Gm23170_chr19_36381832_R | GTCTCGTGGGCTCGGTGTTCCTTGTTTTGTGGCACA | 59.1 | 0.60 |
| mm4_intron_Atf2_chr2_73819571_F | Primer3: not found at this Tm | N.d. | 0.60 |
| mm4_intron_Atf2_chr2_73819571_R | Primer3: not found at this Tm | N.d. | 0.60 |
| mm3_intergenic_Ccdc141|Sestd1_chr2_77171613_F | Primer3: not found at this Tm | N.d. | 0.56 |
| mm3_intergenic_Ccdc141|Sestd1_chr2_77171613_R | Primer3: not found at this Tm | N.d. | 0.56 |
| mm4_intergenic_Lrrtm3|D630028G08Rik_chr10_63948817_F | TCGTCGGCAGCGTCACGCTAAGCATGAGTATCTGTGT | 59.8 | 0.53 |
| mm4_intergenic_Lrrtm3|D630028G08Rik_chr10_63948817_R | GTCTCGTGGGCTCGGGGCCACTCCTACTTCCATGG | 59.8 | 0.53 |
| mm4_intergenic_Gm25577|Gm22537_chr12_118542875_F | Primer3: not found at this Tm | N.d. | 0.53 |
| mm4_intergenic_Gm25577|Gm22537_chr12_118542875_R | Primer3: not found at this Tm | N.d. | 0.53 |
| mm4_intergenic_n-R5s42|Fam19a5_chr15_87215596_F | Primer3: not found at this Tm | N.d. | 0.47 |
| mm4_intergenic_n-R5s42|Fam19a5_chr15_87215596_R | Primer3: not found at this Tm | N.d. | 0.47 |
| mm4_intron_Ppapdc1a_chr7_129277181_F | Primer3: not found at this Tm | N.d. | 0.47 |
| mm4_intron_Ppapdc1a_chr7_129277181_R | Primer3: not found at this Tm | N.d. | 0.47 |
| mm4_intergenic_Sfmbt2|Gm13265_chr2_10641254_F | Primer3: not found at this Tm | N.d. | 0.46 |
| mm4_intergenic_Sfmbt2|Gm13265_chr2_10641254_R | Primer3: not found at this Tm | N.d. | 0.46 |
| mm4_intergenic_Gm14246|Gm22936_chr2_162742447_F | TCGTCGGCAGCGTCGTGACCACTTACAGGCAGCT | 59.9 | 0.40 |
| mm4_intergenic_Gm14246|Gm22936_chr2_162742447_R | GTCTCGTGGGCTCGGTCGTGAGAACTGTCTGGAGC | 59.4 | 0.40 |
| mm4_intergenic_Gm9008|Ctnna2_chr6_76847053_F | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4_intergenic_Gm9008|Ctnna2_chr6_76847053_R | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4_intron_Plxna4_chr6_32227660_F | Primer3: not found at this Tm | N.d. | 0.37 |
| mm4_intron_Plxna4_chr6_32227660_R | Primer3: not found at this Tm | N.d. | 0.37 |
| mm4_exon_Nynrin/Gm26751_chr14_55871435_F | TCGTCGGCAGCGTCTGCAACTGGATCTTCAGCGT | 59.9 | 0.36 |
| mm4_exon_Nynrin/Gm26751_chr14_55871435_R | GTCTCGTGGGCTCGGGATGAGGTAGTGAAGCCCCG | 59.8 | 0.36 |
| mm4_intron_Clnk_chr5_38771606_F | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4_intron_Clnk_chr5_38771606_R | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4_intergenic_Gm4487|Gm24073_chr14_114451567_F | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4_intergenic_Gm4487|Gm24073_chr14_114451567_R | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4_intergenic_Gm13904|Fshb_chr2_107038417_F | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4_intergenic_Gm13904|Fshb_chr2_107038417_R | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4_intergenic_Dysf|Cyp26b1_chr6_84474592_F | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4_intergenic_Dysf|Cyp26b1_chr6_84474592_R | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4_intron_Lyrm4_chr13_36004902_F | TCGTCGGCAGCGTCGCAAACAGGGGAAGTGGCAT | 61.1 | 0.34 |
| mm4_intron_Lyrm4_chr13_36004902_R | GTCTCGTGGGCTCGGTACTGAGAACCAGCAGCTGC | 60.0 | 0.34 |
| mm3_intergenic_Zcchc6|Gas1_chr13_60014351_F | TCGTCGGCAGCGTCGCCAGCTGGATGAACAATGC | 60.1 | 0.33 |
| mm3_intergenic_Zcchc6|Gas1_chr13_60014351_R | GTCTCGTGGGCTCGGAGCAGTGTCCAAGTCTCTCG | 59.3 | 0.33 |
| mm4_exon_Smg1_chr7_118198325_F | TCGTCGGCAGCGTCCCAATGCTCCTCACCACAGT | 59.9 | 0.32 |
| mm4_exon_Smg1_chr7_118198325_R | GTCTCGTGGGCTCGGCTAGGACCTCAGCCATGTGG | 59.5 | 0.32 |
| mm2_intron_Sned1_chr1_93241391_F | Primer3: not found at this Tm | N.d. | 0.31 |
| mm2_intron_Sned1_chr1_93241391_R | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intergenic_Olig3|Ifngr1_chr10_19462150_F | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Olig3|Ifngr1_chr10_19462150_R | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Spz1|Serinc5_chr13_92593271_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intergenic_Spz1|Serinc5_chr13_92593271_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intron_Stxbp5l_chr16_37329347_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intron_Stxbp5l_chr16_37329347_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm3_intergenic_4930552P12Rik|Tcf7l2_chr19_55733091_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm3_intergenic_4930552P12Rik|Tcf7l2_chr19_55733091_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intron_Msi2_chr11_88548268_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intron_Msi2_chr11_88548268_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intron_Sufu_chr19_46477783_F | TCGTCGGCAGCGTCAGTTGCTGTGCTAGGAGCTG | 60.0 | 0.28 |
| mm4_intron_Sufu_chr19_46477783_R | GTCTCGTGGGCTCGGCTTGGTGATCAAGGGTGCGT | 60.6 | 0.28 |
| mm2_intergenic_Foxl1|5033426O07Rik_chr8_121152432_F | TCGTCGGCAGCGTCGTCGCACAGCAAAAGAGCTT | 59.6 | 0.27 |
| mm2_intergenic_Foxl1|5033426O07Rik_chr8_121152432_R | GTCTCGTGGGCTCGGATCCGGGTGAGTCAGTGACT | 60.2 | 0.27 |
| mm4_intergenic_Lsm3|Slc6a6_chr6_91678577_F | TCGTCGGCAGCGTCTCTTGTGTGTCCTTGTCGGG | 59.8 | 0.27 |
| mm4_intergenic_Lsm3|Slc6a6_chr6_91678577_R | GTCTCGTGGGCTCGGACAGAATTGGAGAGCCAGGG | 59.3 | 0.27 |
| mm4_intergenic_Cdh8|Gm15680_chr8_99294887_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Cdh8|Gm15680_chr8_99294887_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm3_exon_Trpc2_chr7_102077766_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm3_exon_Trpc2_chr7_102077766_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Aff1_chr5_103778574_F | TCGTCGGCAGCGTCCTGGCTACAAGGAGAGACGC | 60.1 | 0.22 |
| mm4_intron_Aff1_chr5_103778574_R | GTCTCGTGGGCTCGGAGATGTCACCAGCTCCCTCT | 59.9 | 0.22 |
| mm4_intergenic_Spanxn4|Gm7763_chr12_62689617_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Spanxn4|Gm7763_chr12_62689617_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Gm23960|Gm26373_chr6_21087215_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Gm23960|Gm26373_chr6_21087215_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Gm23083|Gm24773_chr16_80464667_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Gm23083|Gm24773_chr16_80464667_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Gas7_chr11_67500841_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Gas7_chr11_67500841_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Adcy7_chr8_88313429_F | TCGTCGGCAGCGTCGACTGCTGACTCCCATCCAG | 59.8 | 0.21 |
| mm4_intron_Adcy7_chr8_88313429_R | GTCTCGTGGGCTCGGGGAAGGAAGAGGGAAGGTGC | 60.0 | 0.21 |
| mm4_intergenic_Gm23041|Nav3_chr10_109528386_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Gm23041|Nav3_chr10_109528386_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm2_intergenic_Prkch|Hif1a_chr12_73815233_F | TCGTCGGCAGCGTCAGCTGACCTCCTGGCTCATA | 60.0 | 0.19 |
| mm2_intergenic_Prkch|Hif1a_chr12_73815233_R | GTCTCGTGGGCTCGGTCAGACAACTCGGCATTGCT | 59.9 | 0.19 |
| mm4_intron_Prdm2_chr4_143187984_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Prdm2_chr4_143187984_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Csrp2bp_chr2_144403434_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Csrp2bp_chr2_144403434_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Esrrb|uc_338_chr12_86613653_F | TCGTCGGCAGCGTCTGTGTGTGATTTACTGTCCAGGA | 59.6 | 0.18 |
| mm4_intergenic_Esrrb|uc_338_chr12_86613653_R | GTCTCGTGGGCTCGGCATGTGGCCCTCTGTGGTTA | 59.6 | 0.18 |
| mm4_intergenic_Xpnpep1|Add3_chr19_53087720_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Xpnpep1|Add3_chr19_53087720_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Ccnt2|Map3k19_chr1_127809805_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Ccnt2|Map3k19_chr1_127809805_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_AI118078_chr9_55392332_F | TCGTCGGCAGCGTCATAAAACAGAAGCCGCACCG | 59.1 | 0.16 |
| mm4_intron_AI118078_chr9_55392332_R | GTCTCGTGGGCTCGGTTCCAGAGAAGTTCCGGCAC | 59.9 | 0.16 |
| mm4_intergenic_Gm9008|Ctnna2_chr6_76810713_F | TCGTCGGCAGCGTCGCTCACAAGTCATCCAGCCT | 60.0 | 0.16 |
| mm4_intergenic_Gm9008|Ctnna2_chr6_76810713_R | GTCTCGTGGGCTCGGTCTCCAACCCAGTCCTGTCT | 59.8 | 0.16 |
| mm4_intron_Ninl_chr2_150971682_F | TCGTCGGCAGCGTCCTGGTCACTGCAGCCTAACA | 59.9 | 0.16 |
| mm4_intron_Ninl_chr2_150971682_R | GTCTCGTGGGCTCGGGCCCTTTAGAGCCAGACCTG | 60.1 | 0.16 |
| mm4_intron_Espn_chr4_152124875_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Espn_chr4_152124875_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Fam184a|Man1a_chr10_53793250_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Fam184a|Man1a_chr10_53793250_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm3_intergenic_Gm4889|Dusp4_chr8_34514595_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm3_intergenic_Gm4889|Dusp4_chr8_34514595_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Myzap/Gcom1_chr9_71550959_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Myzap/Gcom1_chr9_71550959_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Rreb1_chr13_37793160_F | TCGTCGGCAGCGTCACCAGCAAAGAAACCAATGTGT | 59.2 | 0.13 |
| mm4_intron_Rreb1_chr13_37793160_R | GTCTCGTGGGCTCGGAAGGAACTGGGCCAACGTTT | 60.3 | 0.13 |
| mm4_exon_Dok7_chr5_35079666_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_exon_Dok7_chr5_35079666_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Maf/Gm15655|Dynlrb2_chr8_116171774_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Maf/Gm15655|Dynlrb2_chr8_116171774_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm3_intergenic_Rab17|Lrrfip1_chr1_90972881_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm3_intergenic_Rab17|Lrrfip1_chr1_90972881_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm3_intron_Rmdn1_chr4_19575619_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3_intron_Rmdn1_chr4_19575619_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm4419|Gm5633_chr12_21512727_F | TCGTCGGCAGCGTCCTGAGGCTGACAGAGTTGGG | 60.0 | 0.11 |
| mm4_intergenic_Gm4419|Gm5633_chr12_21512727_R | GTCTCGTGGGCTCGGCTGAACCTTAAGGCCCAGGG | 60.0 | 0.11 |
| mm3_intergenic_Rxrb|Col11a2_chr17_34039128_F | TCGTCGGCAGCGTCGATTACGAGTGGGCGTGGAA | 60.1 | 0.11 |
| mm3_intergenic_Rxrb|Col11a2_chr17_34039128_R | GTCTCGTGGGCTCGGTAGTCCTTTCACACCACGGC | 59.9 | 0.11 |
| mm3_intergenic_Uaca|Gm10655_chr9_61115983_F | TCGTCGGCAGCGTCGACAACTGAGTGCACCATGC | 59.7 | 0.11 |
| mm3_intergenic_Uaca|Gm10655_chr9_61115983_R | GTCTCGTGGGCTCGGTCAGTCATTTGTGCTTGCGC | 60.0 | 0.11 |
| mm3_intergenic_Mvb12b|Gm13403_chr2_33898618_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3_intergenic_Mvb12b|Gm13403_chr2_33898618_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_exon_Zcchc2_chr1_105990540_F | TCGTCGGCAGCGTCGGAAAGGCGGAAGGAGGAC | 60.0 | 0.11 |
| mm4_exon_Zcchc2_chr1_105990540_R | GTCTCGTGGGCTCGGCTCCTCCCCTGGGCTCTG | 60.7 | 0.11 |
| mm4_intergenic_Zfp335|Gm11458/Zfp335_chr2_164911233_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Zfp335|Gm11458/Zfp335_chr2_164911233_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_2900026A02Rik|Tmem211_chr5_113190530_F | TCGTCGGCAGCGTCTCTGCATCATCTCCCCTGGA | 60.0 | 0.10 |
| mm4_intergenic_2900026A02Rik|Tmem211_chr5_113190530_R | GTCTCGTGGGCTCGGAGAACCACACACAGCCTCAG | 59.8 | 0.10 |
| mm4_intergenic_Kars-ps1|Luzp4_chrX_148800728_F | TCGTCGGCAGCGTCGGGGCATCCTCTACATGTCC | 59.6 | 0.10 |
| mm4_intergenic_Kars-ps1|Luzp4_chrX_148800728_R | GTCTCGTGGGCTCGGCCCTTTGGCAGGCCTTGATA | 60.0 | 0.10 |
| mm4_intergenic_Gm8402|Gm5946_chrX_150095716_F | TCGTCGGCAGCGTCGGGGCATCCTCTACATGTCC | 59.6 | 0.10 |
| mm4_intergenic_Gm8402|Gm5946_chrX_150095716_R | GTCTCGTGGGCTCGGCCCTTTGGCAGGCCTTGATA | 60.0 | 0.10 |
| mm4_intergenic_Gm8383|Gm15097_chrX_149728053_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm8383|Gm15097_chrX_149728053_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm8366|Gm15100_chrX_149392702_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm8366|Gm15100_chrX_149392702_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm8346|Gm15093_chrX_149223560_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm8346|Gm15093_chrX_149223560_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm8320|Gm15114_chrX_148440142_F | TCGTCGGCAGCGTCCGGAGTATGGGAATAAGGGGC | 59.9 | 0.10 |
| mm4_intergenic_Gm8320|Gm15114_chrX_148440142_R | GTCTCGTGGGCTCGGCCCTTTGGCAGGCCTTGATA | 60.0 | 0.10 |
| mm4_intergenic_Gm8303|Gm15107_chrX_148120779_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm8303|Gm15107_chrX_148120779_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm15128|Gm15081_chrX_147843235_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm15128|Gm15081_chrX_147843235_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm15107|Gm15109_chrX_148234438_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm15107|Gm15109_chrX_148234438_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm15087|Gm15091_chrX_149897471_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm15087|Gm15091_chrX_149897471_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm15085|Gm15086_chrX_149506039_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm15085|Gm15086_chrX_149506039_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm14708|Gm7155_chrX_75967191_F | TCGTCGGCAGCGTCCCCTTTGGCAGGCCTTGATA | 60.0 | 0.10 |
| mm4_intergenic_Gm14708|Gm7155_chrX_75967191_R | GTCTCGTGGGCTCGGGCATCCCCTACACATCCTCC | 59.6 | 0.10 |
| mm4_exon_Gm8334_chrX_148613258_F | TCGTCGGCAGCGTCGGGGCATCCTCTACATGTCC | 59.6 | 0.10 |
| mm4_exon_Gm8334_chrX_148613258_R | GTCTCGTGGGCTCGGCCCTTTGGCAGGCCTTGATA | 60.0 | 0.10 |
| mm3_intergenic_Wwc1|Gm12125_chr11_35985200_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm3_intergenic_Wwc1|Gm12125_chr11_35985200_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm3_intron_Slc31a2_chr4_62281832_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm3_intron_Slc31a2_chr4_62281832_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Fbxo28|Gm5706_chr1_182368639_F | TCGTCGGCAGCGTCATTCACGATTCTCCTGCCCC | 59.8 | 0.10 |
| mm4_intergenic_Fbxo28|Gm5706_chr1_182368639_R | GTCTCGTGGGCTCGGTATGCCTCCACTGAACTGCC | 59.7 | 0.10 |
| mm4_intron_Ift140_chr17_25094325_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Ift140_chr17_25094325_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Prmt10_chr8_77573777_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Prmt10_chr8_77573777_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_1700036O09Rik|Gm14689_chrX_67473958_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_1700036O09Rik|Gm14689_chrX_67473958_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Bpifb4_chr2_153961176_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Bpifb4_chr2_153961176_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Olfr1219|Olfr1220_chr2_89093617_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Olfr1219|Olfr1220_chr2_89093617_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Phf17_chr3_41557563_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Phf17_chr3_41557563_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm24280|Rbm26/Gm17066_chr14_105074323_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm24280|Rbm26/Gm17066_chr14_105074323_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Rara_chr11_98938025_F | TCGTCGGCAGCGTCCTGGGGTTTCCTGGACTTCC | 59.9 | 0.09 |
| mm4_intron_Rara_chr11_98938025_R | GTCTCGTGGGCTCGGGCCGCAGAAGCCTCAAGG | 61.1 | 0.09 |
| mm4_intergenic_Cry1|AC100386.1_chr10_85228815_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Cry1|AC100386.1_chr10_85228815_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Col8a1_chr16_57630703_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Col8a1_chr16_57630703_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm24968|Gm22797_chr16_71218223_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm24968|Gm22797_chr16_71218223_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Efna1|Gm22642_chr3_89293470_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Efna1|Gm22642_chr3_89293470_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Edem1|Gm20387_chr6_109861770_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Edem1|Gm20387_chr6_109861770_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Ass1_chr2_31509696_F | TCGTCGGCAGCGTCGCGTGTTCATGCATGTGTGT | 60.0 | 0.08 |
| mm4_intron_Ass1_chr2_31509696_R | GTCTCGTGGGCTCGGGGCCTGCAGTTGAAAACCTG | 59.9 | 0.08 |
| mm4_intergenic_Fbxo8|Hand2_chr8_57060432_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Fbxo8|Hand2_chr8_57060432_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Lrrc48_chr11_60382343_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Lrrc48_chr11_60382343_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intron_Tacc2_chr7_130639563_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intron_Tacc2_chr7_130639563_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm15096|Ott_chrX_149021204_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm15096|Ott_chrX_149021204_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm15081|Gm15080_chrX_147939877_F | TCGTCGGCAGCGTCGGGGCATCCTCTACATGTCC | 59.6 | 0.07 |
| mm4_intergenic_Gm15081|Gm15080_chrX_147939877_R | GTCTCGTGGGCTCGGCCCTTTGGCAGGCCTTGATA | 60.0 | 0.07 |
| mm4_intron_Abca17_chr17_24330241_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Abca17_chr17_24330241_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Cnpy1|Rbm33_chr5_28316593_F | TCGTCGGCAGCGTCCCGCGTCTGGAATTCTCACT | 60.1 | 0.07 |
| mm4_intergenic_Cnpy1|Rbm33_chr5_28316593_R | GTCTCGTGGGCTCGGCGGCTTTGAGGACGAGGTTT | 60.6 | 0.07 |
| mm4_intergenic_Med13l|n-R5s175_chr5_119295053_F | TCGTCGGCAGCGTCACAGCCTTACTCAGTCCCCT | 59.8 | 0.07 |
| mm4_intergenic_Med13l|n-R5s175_chr5_119295053_R | GTCTCGTGGGCTCGGTGCACCTGAGCACTGAGTTT | 59.8 | 0.07 |
| mm4_intergenic_Ovol2/1700108N11Rik|Gm11700_chr2_144355122_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Ovol2/1700108N11Rik|Gm11700_chr2_144355122_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm13255|Gm13217_chr2_8958184_F | TCGTCGGCAGCGTCGGCACATACATTCAAAATTAGCACC | 59.4 | 0.07 |
| mm4_intergenic_Gm13255|Gm13217_chr2_8958184_R | GTCTCGTGGGCTCGGTGATGGCCACAACAGATGGT | 59.5 | 0.07 |
| mm4_intron_Prkce_chr17_86409068_F | TCGTCGGCAGCGTCTTTTCAGTGTCTGAGGCCCC | 59.8 | 0.06 |
| mm4_intron_Prkce_chr17_86409068_R | GTCTCGTGGGCTCGGTGAACTGAAGTGGCCCTGAA | 59.1 | 0.06 |
| mm4_intron_Camk2n2_chr16_20620376_F | TCGTCGGCAGCGTCCGACACGGCTCAAAACCCTA | 60.3 | 0.06 |
| mm4_intron_Camk2n2_chr16_20620376_R | GTCTCGTGGGCTCGGCTTCGGAAGAGGGGTGTTCC | 60.0 | 0.06 |
| mm4_intergenic_Gm22227|Zfp703_chr8_26686637_F | TCGTCGGCAGCGTCGCCCTGGAGTGTTCCTCAAT | 59.6 | 0.06 |
| mm4_intergenic_Gm22227|Zfp703_chr8_26686637_R | GTCTCGTGGGCTCGGCTTCAAATGTGCACTGGCCC | 60.0 | 0.06 |
| mm4_intron_Ank2_chr3_127350864_F | TCGTCGGCAGCGTCGACTGTGACCAGGGAAGCTG | 60.3 | 0.06 |
| mm4_intron_Ank2_chr3_127350864_R | GTCTCGTGGGCTCGGCACCTCCTTGTCCTCATGCA | 59.6 | 0.06 |
| mm4_intron_Gm26815_chr8_121342847_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Gm26815_chr8_121342847_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3_intergenic_Gm10288|Ttll7_chr3_146845180_F | TCGTCGGCAGCGTCCCATTGGCTCAGATGCTTGC | 59.8 | 0.05 |
| mm3_intergenic_Gm10288|Ttll7_chr3_146845180_R | GTCTCGTGGGCTCGGCATCAGGATCAGCAGTGCCA | 60.1 | 0.05 |
| mm4_intron_Tshr_chr12_91512046_F | TCGTCGGCAGCGTCACTGGGTAACTGCCACCATC | 59.6 | 0.05 |
| mm4_intron_Tshr_chr12_91512046_R | GTCTCGTGGGCTCGGCCAGATGGCCCTTGTCAAGT | 59.9 | 0.05 |
| mm4_intergenic_Kng2|Gm23715_chr16_23046245_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Kng2|Gm23715_chr16_23046245_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm15611|Tmem243_chr5_9093768_F | TCGTCGGCAGCGTCAAGTGTGCCTAGTGAGCACC | 59.9 | 0.05 |
| mm4_intergenic_Gm15611|Tmem243_chr5_9093768_R | GTCTCGTGGGCTCGGGCTCCTCCATTCACACACCA | 59.9 | 0.05 |
| mm4_intergenic_Zfp648|n-R5s220_chr1_154224061_F | TCGTCGGCAGCGTCTCTAGCTCCATCCCAGCACT | 60.0 | 0.04 |
| mm4_intergenic_Zfp648|n-R5s220_chr1_154224061_R | GTCTCGTGGGCTCGGGTGGGTGAGTGAGTGGACTG | 59.9 | 0.04 |
| mm4_intergenic_Slc6a7|Cdx1_chr18_61017175_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Slc6a7|Cdx1_chr18_61017175_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Ccdc77_chr6_120334275_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Ccdc77_chr6_120334275_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Socs3|Pgs1_chr11_117976341_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Socs3|Pgs1_chr11_117976341_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_Gm24415|Alg10b/mmu-mir-7648_chr15_90214731_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_Gm24415|Alg10b/mmu-mir-7648_chr15_90214731_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Pcsk5_chr19_17470941_F | TCGTCGGCAGCGTCTCCTGGTAAGTTGTGTCCGC | 59.9 | 0.04 |
| mm4_intron_Pcsk5_chr19_17470941_R | GTCTCGTGGGCTCGGAGCCATCCTTCTTTGTCACCT | 59.2 | 0.04 |
| mm4_intergenic_Gm11446|Gm14223_chr2_159883717_F | TCGTCGGCAGCGTCCGGAAAGCAGCTTGAGAGGA | 60.0 | 0.04 |
| mm4_intergenic_Gm11446|Gm14223_chr2_159883717_R | GTCTCGTGGGCTCGGAAAGAAGTAGCCAGGGATAATGA | 57.0 | 0.04 |
| mm4_intergenic_Vwa5b1|Ubxn10_chr4_138678620_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Vwa5b1|Ubxn10_chr4_138678620_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Ppp1r9a_chr6_5110138_F | TCGTCGGCAGCGTCGCATCACTTAGTGGCTGTGC | 59.5 | 0.04 |
| mm4_intron_Ppp1r9a_chr6_5110138_R | GTCTCGTGGGCTCGGTGAAATTCCCAATTCTCCCGGT | 59.9 | 0.04 |
| mm3_exon_Tulp4/Gm5812_chr17_6121816_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_exon_Tulp4/Gm5812_chr17_6121816_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_exon_Gm6211_chr13_103160516_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_exon_Gm6211_chr13_103160516_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_exon_Fbl_chr7_28175034_F | TCGTCGGCAGCGTCGGAGGATGCCCTTGTCACAA | 59.9 | 0.03 |
| mm3_exon_Fbl_chr7_28175034_R | GTCTCGTGGGCTCGGGGCAACGCTATCAGTTGGGA | 60.3 | 0.03 |
| mm3_intergenic_Nap1l2|Gm9050_chrX_103193604_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Nap1l2|Gm9050_chrX_103193604_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Ocm|Lmtk2_chr5_144045842_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Ocm|Lmtk2_chr5_144045842_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_0610010F05Rik_chr11_23582523_F | TCGTCGGCAGCGTCAGAGATCTTAAAGTCACAGATGCA | 57.7 | 0.03 |
| mm4_intron_0610010F05Rik_chr11_23582523_R | GTCTCGTGGGCTCGGATAAGGCCCCACGTATGCAC | 60.1 | 0.03 |
| mm3_intergenic_Slc4a4|Gc_chr5_89264659_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Slc4a4|Gc_chr5_89264659_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm11967|Gck_chr11_5940646_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm11967|Gck_chr11_5940646_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Crtac1_chr19_42293205_F | TCGTCGGCAGCGTCGCCTTGGCGTTCCTGGATAT | 60.1 | 0.03 |
| mm4_intron_Crtac1_chr19_42293205_R | GTCTCGTGGGCTCGGGCGATAGGAGCAGAGTCTGG | 59.6 | 0.03 |
| mm4_intergenic_Hpcal1|B430203G13Rik_chr12_17798455_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Hpcal1|B430203G13Rik_chr12_17798455_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Fer_chr17_63990423_F | TCGTCGGCAGCGTCACAGTATCCACCAAACTGCAGA | 59.6 | 0.02 |
| mm4_intron_Fer_chr17_63990423_R | GTCTCGTGGGCTCGGGTCAGAGGCTGAGGAGGAGA | 60.0 | 0.02 |
| mm4_intron_Sept5_chr16_18625805_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Sept5_chr16_18625805_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm26076|Gm25519_chr3_110709345_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm26076|Gm25519_chr3_110709345_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_AC114591.1|Slco3a1_chr7_74274056_F | TCGTCGGCAGCGTCTGCTGACACCTCAAGGAACA | 59.1 | 0.02 |
| mm4_intergenic_AC114591.1|Slco3a1_chr7_74274056_R | GTCTCGTGGGCTCGGCAACAGCCTCTGTCCTGACA | 59.6 | 0.02 |
| mm3_exon_Blnk_chr19_40965357_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_exon_Blnk_chr19_40965357_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Impg2|Abi3bp_chr16_56344297_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Impg2|Abi3bp_chr16_56344297_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Capn8|4922505E12Rik_chr1_182728007_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Capn8|4922505E12Rik_chr1_182728007_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intron_Prodh2_chr7_30500995_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intron_Prodh2_chr7_30500995_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Glra4|BC065397_chrX_136762907_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Glra4|BC065397_chrX_136762907_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Fstl4_chr11_52906501_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Fstl4_chr11_52906501_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Eapp_chr12_54673147_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Eapp_chr12_54673147_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_n-R5s88|Ephb1_chr9_102181831_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_n-R5s88|Ephb1_chr9_102181831_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_BC034090|Xpr1_chr1_155244272_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_BC034090|Xpr1_chr1_155244272_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Tbx15_chr3_99331380_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Tbx15_chr3_99331380_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Fgd3_chr13_49264834_F | TCGTCGGCAGCGTCAGGGGTAGCCTCACCTGTAG | 60.0 | 0.00 |
| mm4_intron_Fgd3_chr13_49264834_R | GTCTCGTGGGCTCGGAGTCACCTCCTTCCAAGCATG | 59.9 | 0.00 |
| mm3_intron_Spred2_chr11_20006527_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intron_Spred2_chr11_20006527_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm15231|Gm22368/Gm8814_chrX_166995466_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm15231|Gm22368/Gm8814_chrX_166995466_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm25722|Gm10051_chr5_133293620_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm25722|Gm10051_chr5_133293620_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Atp9a_chr2_168659000_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Atp9a_chr2_168659000_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Zfp956|Zfp777_chr6_47977792_F | TCGTCGGCAGCGTCCACGGCCTAGACCACGAC | 59.8 | 0.00 |
| mm4_intergenic_Zfp956|Zfp777_chr6_47977792_R | GTCTCGTGGGCTCGGGTCGAATGACACCGATGCAG | 59.3 | 0.00 |
| mm4_intron_Dock1_chr7_134832183_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Dock1_chr7_134832183_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Jam3|Igsf9b_chr9_27283868_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Jam3|Igsf9b_chr9_27283868_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Kif26a|A730018C14Rik_chr12_112374160_F | TCGTCGGCAGCGTCAGCAGTGGCCTCAGAATCAC | 60.0 | 0.00 |
| mm4_intergenic_Kif26a|A730018C14Rik_chr12_112374160_R | GTCTCGTGGGCTCGGAGTGTGAATCCACCCAGCAC | 60.2 | 0.00 |
| mm4_intergenic_Gja5|Gm24435_chr3_97087868_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gja5|Gm24435_chr3_97087868_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm22593|Mettl4_chr17_94706023_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm22593|Mettl4_chr17_94706023_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Glt1d1|Tmem132d_chr5_127739761_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Glt1d1|Tmem132d_chr5_127739761_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Cdkal1|Gm23729_chr13_29727734_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Cdkal1|Gm23729_chr13_29727734_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_5730508B09Rik|Gm10650_chr3_127992390_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_5730508B09Rik|Gm10650_chr3_127992390_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| mm4_intergenic_Pcgf5|Gm23170_chr19_36381832 |
CTTGAGAACCTGGCCCTGTTTATATGCTCTCCACTGCACCCCAGGCAACTCCCACAGACGGTAGGCAGTCCTCTGGAGAG TCATGGGATGACTTGATAACTCTCACTGCTTATAGTGTGCCACAAAACAAGGAACA |
| mm4_intergenic_Lrrtm3|D630028G08Rik_chr10_63948817 |
ACGCTAAGCATGAGTATCTGTGTTCAGTGATATTAGGCTACCACTGGAGAGTCCGGGAGAGAAAAAAGATATAATGTCAG TGTGATAGTAGCCATGGAAGTAGGAGTGGCC |
| mm4_intergenic_Gm14246|Gm22936_chr2_162742447 |
GTGACCACTTACAGGCAGCTCCCTGCAGACTGGAGAGATTCCCTCGCACATCCCCAGATCCCCTCTGCCACACAAGCCCT CCCTTGCCTTCTCCTGACTCTCCAGAGGCCACAGCAATTAGCTCCAGACAGTTCTCACGA |
| mm4_exon_Nynrin/Gm26751_chr14_55871435 |
TGCAACTGGATCTTCAGCGTTCTCTGGGAGCTCCTGCCCCTCTGGAGAGTCCGGGGCTTCCTGTCATCTGACGGGGCTTC ACTACCTCATC |
| mm4_intron_Lyrm4_chr13_36004902 |
GCAAACAGGGGAAGTGGCATTTACACATTTACATTTTTTCTTTGTGGACCACCTATCTCCATTAGAGAGTCAGGGGATGC AATAGGAAGCTGCACCTGGCAGCTGCTGGTTCTCAGTA |
| mm3_intergenic_Zcchc6|Gas1_chr13_60014351 |
GCCAGCTGGATGAACAATGCTTATGTTTTGAAAGCACTGTGTCCTCTGTAGGATAATGTGGGCTTTGCGAAAACTCCATG CCCAGGCTCTCCAGGGGGCGAGAGACTTGGACACTGCT |
| mm4_exon_Smg1_chr7_118198325 |
CCAATGCTCCTCACCACAGTACTGAACACACGGAGAAGTGCTGCCAGCTTTGGTAATGACACTGATGGAGGAGGAACATC TTCATCCACTGACTCCCCAGAGGCCACATGGCTGAGGTCCTAG |
| mm4_intron_Sufu_chr19_46477783 |
AGTTGCTGTGCTAGGAGCTGCTTAATCAAAGGCCTGTTATGGGCTCATTAGTGTGGTCCACCACGGGCCACCCCTCAGGA GAGTCCTGGGAAGGCTGGCTTAGGTCCCGCCCTTCAGGCACGCACCCTTGATCACCAAG |
| mm2_intergenic_Foxl1|5033426O07Rik_chr8_121152432 |
GTCGCACAGCAAAAGAGCTTTTCTCCTAGACAATCCTGCATCCCCCGACTCTCCAGGGGCTCCGTGTGTTTATAAAAGGC CCCGGGCCTTCTCCCTCACTGGCCTCTATTGACAAATAAGTCACTGACTCACCCGGAT |
| mm4_intergenic_Lsm3|Slc6a6_chr6_91678577 |
TCTTGTGTGTCCTTGTCGGGTCAGGCTGTAGAACCCATCCTTCCCAGGGTTCTCCAGGGGCTTTGCCTCTCCCTTCCCCT CAGGTAGCCCTGGCTCTCCAATTCTGT |
| mm4_intron_Aff1_chr5_103778574 |
CTGGCTACAAGGAGAGACGCGTGAAAGAAGATACTCCTCCCCCGTACTCTCCAAAGGAGAGTTAAAACTTTGAGCATTAA TTCTAGAGGGAGCTGGTGACATCT |
| mm4_intron_Adcy7_chr8_88313429 |
GACTGCTGACTCCCATCCAGGGCCCTCCTTCCCCGAACACTCCTGTGGGGACCCAGTTTCACCCTTGGTCAGCCCCCCTC TGCACCTTCCCTCTTCCTTCC |
| mm2_intergenic_Prkch|Hif1a_chr12_73815233 |
AGCTGACCTCCTGGCTCATAGAGAACTTCTCCACACCAAGATGGCAGAGACACTGGGCCTGCCTTCTCATGCTTTCTGCT GGAGAGTCCAGGGATTGAGGAAGAAGCAATGCCGAGTTGTCTGA |
| mm4_intergenic_Esrrb|uc_338_chr12_86613653 |
TGTGTGTGATTTACTGTCCAGGACTGGGGTCTTCTCCATCCCCTGACACCCCTGGGGCCATACTCTGGATAACCTGTAGT TTTTCTATGATGTAACCACAGAGGGCCACATG |
| mm4_intron_AI118078_chr9_55392332 |
ATAAAACAGAAGCCGCACCGGCGCTCACCTGCTTTCCACCCCCGGACTCCTCTGAGGCCTCATGAGGGGTGCCGGAACTT CTCTGGAA |
| mm4_intergenic_Gm9008|Ctnna2_chr6_76810713 |
GCTCACAAGTCATCCAGCCTCAGGGAAGTTGTTCCTTTCAGGCCACCGGAGAGTCCAGGGTTGGTCATGAAGATTAGCAC AGCTGTGGAAATGAAAAGACAGGACTGGGTTGGAGA |
| mm4_intron_Ninl_chr2_150971682 |
CTGGTCACTGCAGCCTAACACATGAGCGTCTTCCATCACTGGCCTCTCCAAGGGTTTACAACAACTTCCTGCTGCCCTCG GTCTCACAGGTCTGGCTCTAAAGGGC |
| mm4_intron_Rreb1_chr13_37793160 |
ACCAGCAAAGAAACCAATGTGTTCTTAATGGTTTTTACCCAAACCATAAAGCCCTCCCTTGGAGAGACCGGGGAAGCACC CAAACGTTGGCCCAGTTCCTT |
| mm4_intergenic_Gm4419|Gm5633_chr12_21512727 |
CTGAGGCTGACAGAGTTGGGGAAAGCAGCCTCTTCCCTTCCCCTGCCTCTCCAGTGGCCCCTTGTGGGGGCCCTGGGCCT TAAGGTTCAG |
| mm3_intergenic_Rxrb|Col11a2_chr17_34039128 |
GATTACGAGTGGGCGTGGAACGGTCGCCCGTAGGCCCGCCCCTTCTGTCCCACTGTGCCCTGAGTCCCTCCCCGGACTCT TCATAGGGGCTCTGGGCCCCCAAGGGCAGCTGCCGTGGTGTGAAAGGACTA |
| mm3_intergenic_Uaca|Gm10655_chr9_61115983 |
GACAACTGAGTGCACCATGCCAAATAGTATAGGTTTTTTCCCCCATATCCAGATCCTGGGCGCCCCTCGAGAGTTCTGGG ATGGAGGCCACTGCGCAAGCACAAATGACTGA |
| mm4_exon_Zcchc2_chr1_105990540 |
GGAAAGGCGGAAGGAGGACGCGCTCGTCGACTTGCTCCTCCCGCACCGGACTCCCCAGCGGGCACAGAGCCCAGGGGAGG AG |
| mm4_intergenic_2900026A02Rik|Tmem211_chr5_113190530 |
TCTGCATCATCTCCCCTGGAGCAATGGGTGGGATTGTCTAAGTGTTCTAGCCCCAGCCTCTCCAGTGGGTGTTGAGGCAG CCTGAGGCTGTGTGTGGTTCT |
| mm4_intergenic_Kars-ps1|Luzp4_chrX_148800728 |
GGGGCATCCTCTACATGTCCTCCTCTGTAAGAGAGTGAGGATCATCCTATATTTCTTCCTCTGACTCTCCAGAAGAGGAA GAATCAAGGTAATCCAGGTCAGGTATCAAGGCCTGCCAAAGGG |
| mm4_intergenic_Gm8402|Gm5946_chrX_150095716 |
GGGGCATCCTCTACATGTCCTCCTCTGTAAGAGAGTGAGGAACATCCTATATTTCTTCCTCTGACTCTCCAGAAGAGGAA GAATCAAGGTAATCCAGGTCAGGTATCAAGGCCTGCCAAAGGG |
| mm4_intergenic_Gm8320|Gm15114_chrX_148440142 |
CGGAGTATGGGAATAAGGGGCATCCTCTATATGTCCTCCTCTGTAAGAGAGTGAGGAACATCCTATATTTCTTCCTCTGA CTCTCCAGAAGAGGAAGAATCAAGGTAATCCAGGTCAGGTATCAAGGCCTGCCAAAGGG |
| mm4_intergenic_Gm14708|Gm7155_chrX_75967191 |
CCCTTTGGCAGGCCTTGATACCTGACCTGGATTATCTTGTTTCTTTCTCTTCTGGAGAGTCAGAGGAAGAAATATAAGAT GCACCTCACTCTCTTACAGAGGAGGATGTGTAGGGGATGC |
| mm4_exon_Gm8334_chrX_148613258 |
GGGGCATCCTCTACATGTCCTCCTCTGTAAGAGAGTGAGGAACATCCTATATTTCTTCCTCTGACTCTCCAGAAGAGGAA GAATCAAGGTAATCCAGGTCAGGTATCAAGGCCTGCCAAAGGG |
| mm4_intergenic_Fbxo28|Gm5706_chr1_182368639 |
ATTCACGATTCTCCTGCCCCAAACACCAGTTCATCCATCCCCTTACACTCCTGCGGGGAAGGCAGTTCAGTGGAGGCATA |
| mm4_intron_Rara_chr11_98938025 |
CTGGGGTTTCCTGGACTTCCCAGCCTGCCGCTGGCGTGTCCGGGGTTGGGGAGCCCCGTCTGCGCCGCGTCTTCGCGCGC ACAGCCTTGAGGCTTCTGCGGC |
| mm4_intron_Ass1_chr2_31509696 |
GCGTGTTCATGCATGTGTGTGCACAGCCGTCCCCCTCCCCCGACTCCCCAGTGGAAGGGAGGAAGGGCTATTTGGGGCCC TGGGCTCCACCGTAGTTGGTTTAATTACAGGTTTTCAACTGCAGGCC |
| mm4_intergenic_Gm15081|Gm15080_chrX_147939877 |
GGGGCATCCTCTACATGTCCTCCTCTGTAAGAGAGTGAGGATCATCCTATATTTCTTCCACTGACTCTCCAGAAGAGGAA GAATCAAGGTAATCCAGGTCAGGTATCAAGGCCTGCCAAAGGG |
| mm4_intergenic_Cnpy1|Rbm33_chr5_28316593 |
CCGCGTCTGGAATTCTCACTGAGCGGCGACCAGCCTCCATGCCGGGCCTCTCCAAGGGCCCACCTGCGGGGCTCCTCCGC CTTCCCAGCTCGCTTTTGCCCGAAACCTCGTCCTCAAAGCCG |
| mm4_intergenic_Med13l|n-R5s175_chr5_119295053 |
ACAGCCTTACTCAGTCCCCTGGCTGTTCCAGCCGTCCATCACCTATGTCATCCCACTGGAGACTCCGTGTATGAAGCAGA AATATCACCCACCAAAACTCAGTGCTCAGGTGCA |
| mm4_intergenic_Gm13255|Gm13217_chr2_8958184 |
GGCACATACATTCAAAATTAGCACCAGAATGCTTTTAATATATTACTGATATTTATATCCATCCCAAGAGTCTCTAGAGG TAAATAAGTTGAGAAACAATAAGAGTTAATGGCATTCATAACCATCTGTTGTGGCCATCA |
| mm4_intron_Prkce_chr17_86409068 |
TTTTCAGTGTCTGAGGCCCCCAAGGAGTTGACTGAAAGGGACTGAGGAACATCCTCTGGAGAGGCCGGCGAATGAGTAGT TCTCTGGGGAAGCCCAACCTGAAATAAAATACTTCAGGGCCACTTCAGTTCA |
| mm4_intron_Camk2n2_chr16_20620376 |
CGACACGGCTCAAAACCCTATGCCCTCCCTACCCGAACTCTCCCGCGGACCCTACCTGCTACCAAGCCTGGCACCCAAAC CTCCCTATGTGGGAACACCCCTCTTCCGAAG |
| mm4_intergenic_Gm22227|Zfp703_chr8_26686637 |
GCCCTGGAGTGTTCCTCAATAGTGACACATTAGCAAGCAAGTGAGGAGAATGCAAAGATGAACCAAGAAGACTCAATATG TCTGAACACCACTGGGGAGTCGGGGGAGGCATCTGGGGCCAGTGCACATTTGAAG |
| mm4_intron_Ank2_chr3_127350864 |
GACTGTGACCAGGGAAGCTGGCTTCCTGTGGAGAGTGGAGGGATGGAAGCTAGGAGCTTAGATGGTGAAGGTCTGTGTTC TTCATCCAGAAAATGCATGAGGACAAGGAGGTG |
| mm3_intergenic_Gm10288|Ttll7_chr3_146845180 |
CCATTGGCTCAGATGCTTGCTAAGTGCACTGCACACTTCTCTGCTGGAGAGTCCGGGAAGGCAGTCTCCTGGCAGCCAGG CTCTTGGTGATGCTGGCACTGCTGATCCTGATG |
| mm4_intron_Tshr_chr12_91512046 |
ACTGGGTAACTGCCACCATCCCCCACATCTCACTCCCAGGACTATCCAGAGGGTCTCAGTTCTGTTGGGATCTATTTTAA GTATCTCAGGCCTTGAAAATAATGATGAAGGAACTTGACAAGGGCCATCTGG |
| mm4_intergenic_Gm15611|Tmem243_chr5_9093768 |
AAGTGTGCCTAGTGAGCACCAATCGCTGGCATCACCACTGGAGTGCCCACCATCCTCTGGAGGGGCAGGAGATGGAGGCA GCAGCAGGAGGCAGAGATGGGAATGGTGTGTGAATGGAGGAGC |
| mm4_intergenic_Zfp648|n-R5s220_chr1_154224061 |
TCTAGCTCCATCCCAGCACTGGTGCTCTTTGAGGAGTATGAGAATCCATCCCCACCCTCTTCAGAGGAAAGTGTGTTCCA GACAAGTAAGCAGTCCACTCACTCACCCAC |
| mm4_intron_Pcsk5_chr19_17470941 |
TCCTGGTAAGTTGTGTCCGCTCCATCCTTGAACTCTGCAGGGGGTGGGGGTTCGTGGGAACTCACTCTCTTAGTAGGTGA CAAAGAAGGATGGCT |
| mm4_intergenic_Gm11446|Gm14223_chr2_159883717 |
CGGAAAGCAGCTTGAGAGGATTGCCTAAACTAATCTTTAACTTTACTGAAAGGGAAAATTGCTGACATTAATAAGATCCG AAGGAGACTCCAGGGATGGATCATTATCCCTGGCTACTTCTTT |
| mm4_intron_Ppp1r9a_chr6_5110138 |
GCATCACTTAGTGGCTGTGCTCAGCTGCAGAGCAGAAAATGTTCATGGGCTTGGCTGCAAAGTGTCTCCCTCCCCAGACC CTCCACTGGGCTGTTTGGACCTATAAAACCGGGAGAATTGGGAATTTCA |
| mm3_exon_Fbl_chr7_28175034 |
GGAGGATGCCCTTGTCACAAAGAATCTGGTCCCTGGAGAGTCTGTGTATGGAGAGAAGAGAGTCTCTATTTCAGTGAGTC CAAGTCCCAACTGATAGCGTTGCC |
| mm4_intron_0610010F05Rik_chr11_23582523 |
AGAGATCTTAAAGTCACAGATGCATAAATAACACATTACCCCTGGACAGTCTGGTGATGAACTATGATTGGTGTCTAGAG AACGTGCATACGTGGGGCCTTAT |
| mm4_intron_Crtac1_chr19_42293205 |
GCCTTGGCGTTCCTGGATATGACAGGACACCCACTGTCTACCACAGCCTTTCCAAGCCTCCCCTGGAGACCCCAGGGAGG GAGCTTGGCCCAGCTCCCACTTTCCAGACTCTGCTCCTATCGC |
| mm4_intron_Fer_chr17_63990423 |
ACAGTATCCACCAAACTGCAGAATATCCATCCGCCTCCTCTCCAGTGGGATTTTGGGCTGTTAATCTAGCTTCTTTCTGC TTCTTTCATGTTCTCCTCCTCAGCCTCTGAC |
| mm4_intergenic_AC114591.1|Slco3a1_chr7_74274056 |
TGCTGACACCTCAAGGAACAGCTAGCTCTTCTGCATCCACTGACTTTCCAGAGGTGAAACCAGCTCCCTTAGGAAGGAAA CACTAACCACTTTTTCAGCTGATTCAGTATCTTTTCATGTCAGGACAGAGGCTGTTG |
| mm4_intron_Fgd3_chr13_49264834 |
AGGGGTAGCCTCACCTGTAGTGATGTCTTTGTCCTCTGCAGAGGCTGGGGCTGGATCATGGACCATGCTTGGAAGGAGGT GACT |
| mm4_intergenic_Zfp956|Zfp777_chr6_47977792 |
CACGGCCTAGACCACGACCGCGGCCCCAAGACCGCTGCGGAATCCTCTGCAGAAGCCGGCGATGGACTCCTAATCCTGGG CCCCCGGGTCCTCCGGACCCTCCCGCTGCATCGGTGTCATTCGAC |
| mm4_intergenic_Kif26a|A730018C14Rik_chr12_112374160 |
AGCAGTGGCCTCAGAATCACCTCGTGCGACTGCACAGCCCTGCCCTTTAGCCAAGCCCTGGCAGTACCATCTGTAGGCTG GGAACCACTCCAGGGTCAGGGGATGGAAGCCAAGGTGTGCTGGGTGGATTCACACT |