CRISPOR

DNA break repair outcome predictions

Guide: AGCAGGCAGTGCTGCAGGGC TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GAGGAGCCGGTCGATGGACTAGCAGGCAGTGCTGCAGGGCTGGGCGCCGAGCCACGGTCTGCTGGAGCGGCCATGCTTGG`	(Wild-type sequence)
623	`TCGATGGACTAGCAGGCAGTGCTGCAGGGC-----GCCGAGCCACGGTCTGCTGGAGCGG`	`5 bp deleted → frameshift`
327	`TCGATGGACTAGCAGGCAGTGCTGCAGGG----GCGCCGAGCCACGGTCTGCTGGAGCGG`	`4 bp deleted → frameshift`
296	`TCGATGGACTAGCAGGCAGTGCTGCAGG------CGCCGAGCCACGGTCTGCTGGAGCGG`	`6 bp deleted → no frameshift`
282	`TCGATGGACTAGCAGGCAGTGCTGCAGGGC-------CGAGCCACGGTCTGCTGGAGCGG`	`7 bp deleted → frameshift`
244	`TCGATGGACTAGCAGGC------------------GCCGAGCCACGGTCTGCTGGAGCGG`	`18 bp deleted → no frameshift`
242	`TCGATGGACTAGCAGGCAGTGCTGC----------GCCGAGCCACGGTCTGCTGGAGCGG`	`10 bp deleted → frameshift`
219	`TCGATGGACTAGCAGGCAGTGCTGCAGGGC------------CACGGTCTGCTGGAGCGG`	`12 bp deleted → no frameshift`
219	`TCGATGGACTAGCAGGCAGTGCTGC------------CGAGCCACGGTCTGCTGGAGCGG`	`12 bp deleted → no frameshift`
208	`TCGATGGACTAGCAGGCAGTGC-------------GCCGAGCCACGGTCTGCTGGAGCGG`	`13 bp deleted → frameshift`
201	`TCGATGGACTAGCAGGCAGTGCTG--------GGCGCCGAGCCACGGTCTGCTGGAGCGG`	`8 bp deleted → frameshift`
188	`TCGATGGACTAGCAGGCAGTGC---------------CGAGCCACGGTCTGCTGGAGCGG`	`15 bp deleted → no frameshift`
181	`TCGATGGACTAGCAGGCAGTGCTGC---------------------------TGGAGCGG`	`27 bp deleted → no frameshift`
178	`TCGATGGACTAGCAGGCAGTGCTG------------------------------GAGCGG`	`30 bp deleted → no frameshift`
173	`TCGATGGACTAGCAGGCAGTG-----------GGCGCCGAGCCACGGTCTGCTGGAGCGG`	`11 bp deleted → frameshift`
170	`TCGATGGACTAGCAGGCAGTGCTGC-----------------CACGGTCTGCTGGAGCGG`	`17 bp deleted → frameshift`
170	`TCGATGGACTAGCAGG-----------------GCGCCGAGCCACGGTCTGCTGGAGCGG`	`17 bp deleted → frameshift`
162	`TCGATGGACTAGCAGGCAGTGCTGCAGGG------------------TCTGCTGGAGCGG`	`18 bp deleted → no frameshift`
154	`TCGATGGACTAGCAGGCAGTGCTGCAGG-------------------TCTGCTGGAGCGG`	`19 bp deleted → frameshift`
149	`TCGATGGACTAGCAGGCAGTGCTGCAG--------------CCACGGTCTGCTGGAGCGG`	`14 bp deleted → frameshift`
147	`TCGATGGACTAGCAGGCAGTGC--------------------CACGGTCTGCTGGAGCGG`	`20 bp deleted → frameshift`
147	`TCGATGGACTAGCAGGC--------------------CGAGCCACGGTCTGCTGGAGCGG`	`20 bp deleted → frameshift`
136	`TCGATGG--------------------------GCGCCGAGCCACGGTCTGCTGGAGCGG`	`26 bp deleted → frameshift`
133	`TCGATGGACTAGCAGGCAGTGCTGCAGGGC----------------------TGGAGCGG`	`22 bp deleted → frameshift`
133	`TCGATGGACTAGC----------------------GCCGAGCCACGGTCTGCTGGAGCGG`	`22 bp deleted → frameshift`
122	`TCGATGGACTAGCAGGCAGTGCTGCA------------------CGGTCTGCTGGAGCGG`	`18 bp deleted → no frameshift`
120	`TCGATGGACTAGC------------------------CGAGCCACGGTCTGCTGGAGCGG`	`24 bp deleted → no frameshift`
117	`TCGATGGACTAGC-----------------------------CACGGTCTGCTGGAGCGG`	`29 bp deleted → frameshift`
114	`TCGATGGACTAGCAGGC-------------------------CACGGTCTGCTGGAGCGG`	`25 bp deleted → frameshift`
109	`TCGATGGACTAGCAGGCAGTGCTGCAGGG--------------------------AGCGG`	`26 bp deleted → frameshift`
103	`TCGATGGACTAGCAGGCAGTGCTGCAGG---------------------------AGCGG`	`27 bp deleted → no frameshift`
103	`TCGATGG---------------------------CGCCGAGCCACGGTCTGCTGGAGCGG`	`27 bp deleted → no frameshift`
99	`TCGATGGACTAGCAGGCAG----------------------CCACGGTCTGCTGGAGCGG`	`22 bp deleted → frameshift`
98	`TCGATGGACTAGCAGGCAGTGCTGCAGGGC----------------------------GG`	`28 bp deleted → frameshift`
84	`TCGATGGACTAGCAGGCAGTGCTGCAGGG-------------------------------`	`31 bp deleted → frameshift`
84	`TCGATGGACTAGCAGG-------------------------------TCTGCTGGAGCGG`	`31 bp deleted → frameshift`
82	`TCGA------------------------------------GCCACGGTCTGCTGGAGCGG`	`36 bp deleted → no frameshift`
81	`TCGATGGACTAGCAGGCA--------------------------CGGTCTGCTGGAGCGG`	`26 bp deleted → frameshift`
81	`TCGATGGACTAGCAG--------------------------CCACGGTCTGCTGGAGCGG`	`26 bp deleted → frameshift`
80	`TCGATGGACTAGCAGGCAGTGCTGCAGG--------------------------------`	`32 bp deleted → frameshift`
76	`TCGATGGACTAGCAGGCAGTGCTGC---------------------------------GG`	`33 bp deleted → no frameshift`
76	`TCG---------------------------------CCGAGCCACGGTCTGCTGGAGCGG`	`33 bp deleted → no frameshift`
74	`TCGATGGACTAGCAGGCAGT----------------------------CTGCTGGAGCGG`	`28 bp deleted → frameshift`
69	`TCGATGGACTAGCAGGC-----------------------------------TGGAGCGG`	`35 bp deleted → frameshift`
66	`TCGATGGACTAGCAGGCAGTGCTGCAG------------------------------CGG`	`30 bp deleted → no frameshift`
66	`TCGATGGACTAGCA------------------------------CGGTCTGCTGGAGCGG`	`30 bp deleted → no frameshift`
66	`TCGATGGACTAGCAGGCAGTGC------------------------------------GG`	`36 bp deleted → no frameshift`
60	`TCGATGGA--------------------------------GCCACGGTCTGCTGGAGCGG`	`32 bp deleted → frameshift`
57	`TCGATGGACTAGCAGGCAGTG---------------------------------GAGCGG`	`33 bp deleted → no frameshift`
56	`TCGATGGACTAGCAGG---------------------------------------AGCGG`	`39 bp deleted → no frameshift`
56	`TCGATGGACTAGC---------------------------------------TGGAGCGG`	`39 bp deleted → no frameshift`
54	`TCGATGGA------------------------------------------------GCGG`	`48 bp deleted → no frameshift`
54	`TCGATGG----------------------------------------TCTGCTGGAGCGG`	`40 bp deleted → frameshift`
52	`TCGATGGACTAGC---------------------------------------------GG`	`45 bp deleted → no frameshift`
51	`TCGATGGACTAGCAGGC-----------------------------------------GG`	`41 bp deleted → frameshift`
49	`TCGATGGAC------------------------------------GGTCTGCTGGAGCGG`	`36 bp deleted → no frameshift`
46	`TCG-------------------------------------------GTCTGCTGGAGCGG`	`43 bp deleted → frameshift`
45	`TCGATGGACTAGCAGGCAG--------------------------------------CGG`	`38 bp deleted → frameshift`
44	`TCGATGGACTAGCAGG--------------------------------------------`	`44 bp deleted → frameshift`
40	`TCGATGGACT----------------------------------------GCTGGAGCGG`	`40 bp deleted → frameshift`
36	`TCGATGGACTAGCAG------------------------------------------CGG`	`42 bp deleted → no frameshift`
34	`TCGATGGACT-------------------------------------------GGAGCGG`	`43 bp deleted → frameshift`
31	`TCGATG---------------------------------------------CTGGAGCGG`	`45 bp deleted → no frameshift`
28	`TC-----------------------------------------------TGCTGGAGCGG`	`47 bp deleted → frameshift`
28	`TCGATGG-----------------------------------------------------`	`53 bp deleted → frameshift`
24	`TCG--------------------------------------------------------G`	`56 bp deleted → frameshift`
22	`TCGA----------------------------------------------------GCGG`	`52 bp deleted → frameshift`

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