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Contents:

PCR primers for off-targets of CAGGGCTGGGCGCCGAGCCA CGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314076_F Primer3: not found at this Tm N.d. 1.00
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314076_R Primer3: not found at this Tm N.d. 1.00
mm4_intergenic_Gm22965|Ift81_chr5_122512138_F Primer3: not found at this Tm N.d. 0.58
mm4_intergenic_Gm22965|Ift81_chr5_122512138_R Primer3: not found at this Tm N.d. 0.58
mm4_intergenic_Phex|Gm1947_chrX_157421761_F Primer3: not found at this Tm N.d. 0.51
mm4_intergenic_Phex|Gm1947_chrX_157421761_R Primer3: not found at this Tm N.d. 0.51
mm3_intergenic_Tbc1d22a|Gm23416_chr15_86577417_F Primer3: not found at this Tm N.d. 0.51
mm3_intergenic_Tbc1d22a|Gm23416_chr15_86577417_R Primer3: not found at this Tm N.d. 0.51
mm3_exon_Tmem8_chr17_26113430_F Primer3: not found at this Tm N.d. 0.50
mm3_exon_Tmem8_chr17_26113430_R Primer3: not found at this Tm N.d. 0.50
mm4_intron_Vstm2l_chr2_157921742_F TCGTCGGCAGCGTCGAGTGTGTAGGCAGTCCCTG 59.7 0.48
mm4_intron_Vstm2l_chr2_157921742_R GTCTCGTGGGCTCGGCCTGTGGGTTCTTTCAGGCA 60.1 0.48
mm4_intergenic_Agap1|Gm25180_chr1_89462071_F TCGTCGGCAGCGTCTGCAGGTCAGTGTTAGCCAC 60.2 0.40
mm4_intergenic_Agap1|Gm25180_chr1_89462071_R GTCTCGTGGGCTCGGTGTGAGCAAGGCCTGAGATC 59.7 0.40
mm4_intron_Aagab_chr9_63610343_F Primer3: not found at this Tm N.d. 0.39
mm4_intron_Aagab_chr9_63610343_R Primer3: not found at this Tm N.d. 0.39
mm3_intergenic_Nphp4|Gm13172_chr4_152617015_F Primer3: not found at this Tm N.d. 0.34
mm3_intergenic_Nphp4|Gm13172_chr4_152617015_R Primer3: not found at this Tm N.d. 0.34
mm4_intron_Itgb2l_chr16_96434098_F TCGTCGGCAGCGTCTAAGTGCAAGTTCAGGGCCC 60.2 0.32
mm4_intron_Itgb2l_chr16_96434098_R GTCTCGTGGGCTCGGTCGGTCCCAGGCTCTCTTTA 59.9 0.32
mm4_intergenic_Gm23926|Gm25115_chr14_86395723_F TCGTCGGCAGCGTCCAGCTGGCGATCAGTCTTCT 59.8 0.32
mm4_intergenic_Gm23926|Gm25115_chr14_86395723_R GTCTCGTGGGCTCGGAGAGGCATTTCAGACCCTGC 60.0 0.32
mm4_intergenic_Gm16232|Pigk_chr3_152893378_F Primer3: not found at this Tm N.d. 0.30
mm4_intergenic_Gm16232|Pigk_chr3_152893378_R Primer3: not found at this Tm N.d. 0.30
mm4_exon_Srrm3_chr5_135857187_F TCGTCGGCAGCGTCCAGAACGGAGGCAGCAGG 60.4 0.29
mm4_exon_Srrm3_chr5_135857187_R GTCTCGTGGGCTCGGACAAACCCTCCACAAAGCCA 60.0 0.29
mm4_intron_Gpr133_chr5_129174821_F TCGTCGGCAGCGTCTCAGAAGGAGATGGAGTGGGT 59.9 0.28
mm4_intron_Gpr133_chr5_129174821_R GTCTCGTGGGCTCGGAGCAGAACCATCAGCTGAGT 59.0 0.28
mm4_intron_Efcab6_chr15_84006913_F Primer3: not found at this Tm N.d. 0.26
mm4_intron_Efcab6_chr15_84006913_R Primer3: not found at this Tm N.d. 0.26
mm4_intergenic_Tinagl1|Gm853_chr4_130192424_F Primer3: not found at this Tm N.d. 0.25
mm4_intergenic_Tinagl1|Gm853_chr4_130192424_R Primer3: not found at this Tm N.d. 0.25
mm4_intron_Vps13d_chr4_145193535_F TCGTCGGCAGCGTCACACAATGTGAAGCTGACTACCT 59.9 0.25
mm4_intron_Vps13d_chr4_145193535_R GTCTCGTGGGCTCGGGGAGAGTTGGCTTTTAGGGCT 59.9 0.25
mm4_intron_Skap1_chr11_96657296_F Primer3: not found at this Tm N.d. 0.23
mm4_intron_Skap1_chr11_96657296_R Primer3: not found at this Tm N.d. 0.23
mm4_intron_Cecr5_chr6_120521901_F TCGTCGGCAGCGTCCTCGGACCTCAGCATTGTGT 60.0 0.23
mm4_intron_Cecr5_chr6_120521901_R GTCTCGTGGGCTCGGAGCCAGTCCTTCTCGATTGC 60.1 0.23
mm4_intron_Zfp384_chr6_125014505_F Primer3: not found at this Tm N.d. 0.21
mm4_intron_Zfp384_chr6_125014505_R Primer3: not found at this Tm N.d. 0.21
mm4_intron_Nav2_chr7_49192026_F Primer3: not found at this Tm N.d. 0.21
mm4_intron_Nav2_chr7_49192026_R Primer3: not found at this Tm N.d. 0.21
mm3_intron_Ccz1_chr5_143998511_F TCGTCGGCAGCGTCGAGAGCTCCCTTTCGGATCG 59.9 0.21
mm3_intron_Ccz1_chr5_143998511_R GTCTCGTGGGCTCGGGCCTGGGAATCTTCAGCACT 60.0 0.21
mm4_intergenic_AI182371|Cep110_chr2_35107522_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_AI182371|Cep110_chr2_35107522_R Primer3: not found at this Tm N.d. 0.19
mm4_intron_Ptcd1_chr5_145153088_F Primer3: not found at this Tm N.d. 0.19
mm4_intron_Ptcd1_chr5_145153088_R Primer3: not found at this Tm N.d. 0.19
mm4_intron_Itpr1_chr6_108536882_F Primer3: not found at this Tm N.d. 0.19
mm4_intron_Itpr1_chr6_108536882_R Primer3: not found at this Tm N.d. 0.19
mm3_intergenic_Mfsd11|Mgat5b_chr11_116918706_F Primer3: not found at this Tm N.d. 0.19
mm3_intergenic_Mfsd11|Mgat5b_chr11_116918706_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm12158|Ebf1_chr11_44823988_F TCGTCGGCAGCGTCTCGTGGCAGAATTTCATAAGTGA 58.4 0.18
mm4_intergenic_Gm12158|Ebf1_chr11_44823988_R GTCTCGTGGGCTCGGCAAAGTCTCACACACTCTAGGC 58.6 0.18
mm4_intergenic_Kif12|Col27a1_chr4_63186784_F Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Kif12|Col27a1_chr4_63186784_R Primer3: not found at this Tm N.d. 0.18
mm4_intron_Tyk2_chr9_21118216_F Primer3: not found at this Tm N.d. 0.16
mm4_intron_Tyk2_chr9_21118216_R Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Trappc10|Agpat3_chr10_78252121_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Trappc10|Agpat3_chr10_78252121_R Primer3: not found at this Tm N.d. 0.15
mm4_intron_Cdh13_chr8_119288482_F TCGTCGGCAGCGTCGCATGATCAGAGAGCAGCCT 59.8 0.15
mm4_intron_Cdh13_chr8_119288482_R GTCTCGTGGGCTCGGGCCGTGTACCGAGCATAGAA 59.8 0.15
mm3_intron_Osbpl2_chr2_180143412_F Primer3: not found at this Tm N.d. 0.14
mm3_intron_Osbpl2_chr2_180143412_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Gm24365|Bag3_chr7_128514351_F TCGTCGGCAGCGTCGCCCAGCCCTAACTAGGAGA 60.3 0.14
mm4_intergenic_Gm24365|Bag3_chr7_128514351_R GTCTCGTGGGCTCGGTGTGTTATACAGCCGTGGGG 59.7 0.14
mm4_intron_Megf11_chr9_64485140_F TCGTCGGCAGCGTCCAGCTGACTGGACTTGGAGG 60.0 0.14
mm4_intron_Megf11_chr9_64485140_R GTCTCGTGGGCTCGGCGGGGACAGAATCTGCTGAC 60.4 0.14
mm4_intergenic_Bicc1|Tfam_chr10_71224828_F TCGTCGGCAGCGTCCCCACAGGAGGTAGTCAGGA 59.9 0.14
mm4_intergenic_Bicc1|Tfam_chr10_71224828_R GTCTCGTGGGCTCGGGCTAGGATAAGGAGCCTGCG 60.0 0.14
mm3_intron_Rnf180_chr13_105247771_F Primer3: not found at this Tm N.d. 0.14
mm3_intron_Rnf180_chr13_105247771_R Primer3: not found at this Tm N.d. 0.14
mm4_intron_Nckap5_chr1_126593451_F Primer3: not found at this Tm N.d. 0.13
mm4_intron_Nckap5_chr1_126593451_R Primer3: not found at this Tm N.d. 0.13
mm4_intron_Tbx4_chr11_85912703_F TCGTCGGCAGCGTCCAACACTGACCTGGGACCAG 60.2 0.13
mm4_intron_Tbx4_chr11_85912703_R GTCTCGTGGGCTCGGAGGATCTTGAGGACCCACGA 59.9 0.13
mm4_exon_Dleu7/Rnaseh2b_chr14_62292782_F TCGTCGGCAGCGTCGATGCTCCCGCGTCCCAG 62.8 0.13
mm4_exon_Dleu7/Rnaseh2b_chr14_62292782_R GTCTCGTGGGCTCGGTACTACAGCAGGAGTGGGGC 60.9 0.13
mm4_intergenic_Cbx6/Nptxr/Npcd|Apobec3_chr15_79848179_F TCGTCGGCAGCGTCTCCGCCGATCATTCCATACC 59.6 0.13
mm4_intergenic_Cbx6/Nptxr/Npcd|Apobec3_chr15_79848179_R GTCTCGTGGGCTCGGCACTGGGATGCTGGCTAGAG 59.8 0.13
mm4_exon_Tcof1_chr18_60832425_F TCGTCGGCAGCGTCGGCTGAGACCTCTGAGGACT 60.3 0.12
mm4_exon_Tcof1_chr18_60832425_R GTCTCGTGGGCTCGGGCAGCTCTCAGGGTCCAAAT 60.0 0.12
mm4_intron_Smek2_chr11_29189434_F Primer3: not found at this Tm N.d. 0.12
mm4_intron_Smek2_chr11_29189434_R Primer3: not found at this Tm N.d. 0.12
mm4_intron_Kctd1_chr18_15104561_F Primer3: not found at this Tm N.d. 0.12
mm4_intron_Kctd1_chr18_15104561_R Primer3: not found at this Tm N.d. 0.12
mm3_intergenic_6330418K02Rik|Gal3st4/Gpc2/6330418K02Rik_chr5_138264413_F Primer3: not found at this Tm N.d. 0.11
mm3_intergenic_6330418K02Rik|Gal3st4/Gpc2/6330418K02Rik_chr5_138264413_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Ppp2r3a|Gm24638_chr9_101276210_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Ppp2r3a|Gm24638_chr9_101276210_R Primer3: not found at this Tm N.d. 0.11
mm4_intron_Sbf1_chr15_89313542_F TCGTCGGCAGCGTCCTCACCACCATCCAAGTCCA 59.3 0.11
mm4_intron_Sbf1_chr15_89313542_R GTCTCGTGGGCTCGGGAGGAAACCAAGCTGGCTGA 60.2 0.11
mm4_intergenic_Zfp428|Irgq_chr7_24529675_F TCGTCGGCAGCGTCGGTCATTCCTGGCAGGTAGT 59.0 0.10
mm4_intergenic_Zfp428|Irgq_chr7_24529675_R GTCTCGTGGGCTCGGACTGCCAGAAAGAGGGGTTG 59.8 0.10
mm4_intergenic_Gm26674/Sash1|Gm9930_chr10_8916734_F TCGTCGGCAGCGTCCGGAGGTGATCGCTTTGTTT 58.8 0.10
mm4_intergenic_Gm26674/Sash1|Gm9930_chr10_8916734_R GTCTCGTGGGCTCGGAGCGTACAGACAGCATAGCA 59.1 0.10
mm4_intron_Grik4_chr9_42720790_F TCGTCGGCAGCGTCGTTTGGCCTGTTCTGCACTG 59.9 0.09
mm4_intron_Grik4_chr9_42720790_R GTCTCGTGGGCTCGGGGCGGGATCTTTACGCTCTA 59.3 0.09
mm4_intron_Sh3bp4_chr1_89125777_F TCGTCGGCAGCGTCAGGAAGAGGGTGGTTTGTGC 60.1 0.09
mm4_intron_Sh3bp4_chr1_89125777_R GTCTCGTGGGCTCGGTGCAGATACTGACAAGTAGTATCGA 59.1 0.09
mm4_intron_Tll2_chr19_41189268_F TCGTCGGCAGCGTCTGAGGTCACAGGAACCGAGA 60.1 0.09
mm4_intron_Tll2_chr19_41189268_R GTCTCGTGGGCTCGGCATGCGGTGCCAAAGTGAAG 60.3 0.09
mm4_intergenic_Hbq1a|Sh3pxd2b_chr11_32307316_F TCGTCGGCAGCGTCTCACAGTAACAAGGCAGGCC 60.2 0.09
mm4_intergenic_Hbq1a|Sh3pxd2b_chr11_32307316_R GTCTCGTGGGCTCGGCTTCACCCACCTGCTTCCTC 60.3 0.09
mm3_intergenic_Map3k7cl|Gm22808_chr16_87604086_F Primer3: not found at this Tm N.d. 0.09
mm3_intergenic_Map3k7cl|Gm22808_chr16_87604086_R Primer3: not found at this Tm N.d. 0.09
mm4_intron_Fmo3_chr1_162957595_F TCGTCGGCAGCGTCGTTGCCCACAGCTATGTCCT 60.0 0.09
mm4_intron_Fmo3_chr1_162957595_R GTCTCGTGGGCTCGGGCAGCCAGCATCCTCTCATA 59.6 0.09
mm3_intergenic_Ttyh3|Iqce_chr5_140659731_F TCGTCGGCAGCGTCAGGAAGTAGCCGAGTGGAGT 59.9 0.09
mm3_intergenic_Ttyh3|Iqce_chr5_140659731_R GTCTCGTGGGCTCGGCCTCCCTTGGCACATGTGAT 60.0 0.09
mm4_intron_Foxo3_chr10_42205329_F TCGTCGGCAGCGTCTCCATTCCTTCCAGCCAAGC 60.3 0.09
mm4_intron_Foxo3_chr10_42205329_R GTCTCGTGGGCTCGGGTGGAGTGGGAAGTGTGAGG 59.9 0.09
mm3_intergenic_Wnt7b|AU022754/Wnt7b_chr15_85581015_F Primer3: not found at this Tm N.d. 0.08
mm3_intergenic_Wnt7b|AU022754/Wnt7b_chr15_85581015_R Primer3: not found at this Tm N.d. 0.08
mm4_intron_Adamts14_chr10_61261833_F TCGTCGGCAGCGTCACTCCCCTATGCCACTCCAT 60.0 0.08
mm4_intron_Adamts14_chr10_61261833_R GTCTCGTGGGCTCGGCACTTCCTGTCTCCTCGTCC 59.4 0.08
mm4_intergenic_Pgm2|Gm12702_chr4_99992035_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Pgm2|Gm12702_chr4_99992035_R Primer3: not found at this Tm N.d. 0.08
mm4_intron_Triobp_chr15_78988644_F Primer3: not found at this Tm N.d. 0.08
mm4_intron_Triobp_chr15_78988644_R Primer3: not found at this Tm N.d. 0.08
mm4_exon_Skiv2l/mmu-mir-6970_chr17_34845137_F TCGTCGGCAGCGTCGCCTCCACTGCAGCATAGTA 59.5 0.08
mm4_exon_Skiv2l/mmu-mir-6970_chr17_34845137_R GTCTCGTGGGCTCGGCTCTTCCTGCTGCTGGACTC 60.1 0.08
mm4_intron_Msi2_chr11_88532488_F TCGTCGGCAGCGTCCATAGACCTCGCACGTCTCC 59.9 0.08
mm4_intron_Msi2_chr11_88532488_R GTCTCGTGGGCTCGGAACAGCTCTGGCCTTGTCTC 59.9 0.08
mm4_exon_Nr5a1_chr2_38692773_F TCGTCGGCAGCGTCAGGGCACTTCTTGTCTTGGG 59.8 0.08
mm4_exon_Nr5a1_chr2_38692773_R GTCTCGTGGGCTCGGTTATGCTAGGCCCACCCAAC 59.7 0.08
mm4_intron_C130046K22Rik_chr11_103711861_F Primer3: not found at this Tm N.d. 0.07
mm4_intron_C130046K22Rik_chr11_103711861_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm12315|Gp1ba_chr11_70632218_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm12315|Gp1ba_chr11_70632218_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm15729|Nrros_chr16_32117131_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm15729|Nrros_chr16_32117131_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm22704|Gm14234_chr2_168399482_F TCGTCGGCAGCGTCTGAATGTCGAGAGCCATGGG 59.8 0.07
mm4_intergenic_Gm22704|Gm14234_chr2_168399482_R GTCTCGTGGGCTCGGCCACAACTCTGCTCCCTGTT 59.8 0.07
mm4_intron_Ngf_chr3_102488717_F TCGTCGGCAGCGTCTGCGAGGCTCACATTGTTCT 59.9 0.07
mm4_intron_Ngf_chr3_102488717_R GTCTCGTGGGCTCGGTGTGCTCAGGGACAAAGTGG 60.1 0.07
mm4_intron_Fmn2_chr1_174568909_F Primer3: not found at this Tm N.d. 0.07
mm4_intron_Fmn2_chr1_174568909_R Primer3: not found at this Tm N.d. 0.07
mm4_exon_Tead3_chr17_28336241_F Primer3: not found at this Tm N.d. 0.07
mm4_exon_Tead3_chr17_28336241_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_n-R5s42|Fam19a5_chr15_87598823_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_n-R5s42|Fam19a5_chr15_87598823_R Primer3: not found at this Tm N.d. 0.06
mm3_intergenic_Gm23702|Kirrel3_chr9_34977897_F TCGTCGGCAGCGTCCACATGAGCAAGGGCAGTG 59.1 0.06
mm3_intergenic_Gm23702|Kirrel3_chr9_34977897_R GTCTCGTGGGCTCGGGTTTGAGGGGAGATGGCTGT 59.6 0.06
mm4_intergenic_RP23-387F1.3|E030018B13Rik_chr7_63902032_F TCGTCGGCAGCGTCATCCAGAGGAAACCAGTGCC 59.6 0.06
mm4_intergenic_RP23-387F1.3|E030018B13Rik_chr7_63902032_R GTCTCGTGGGCTCGGGCCAACTGCTACAACCCAGT 60.5 0.06
mm4_intergenic_Osmr|Lifr_chr15_6919705_F TCGTCGGCAGCGTCTTAGAGAGACACGTGGCTGC 59.7 0.06
mm4_intergenic_Osmr|Lifr_chr15_6919705_R GTCTCGTGGGCTCGGTGGGACCGTGAGAAACAGAG 59.3 0.06
mm4_exon_Sdk2_chr11_114066590_F TCGTCGGCAGCGTCCACCTCCGGCTAGCAAACTT 60.3 0.06
mm4_exon_Sdk2_chr11_114066590_R GTCTCGTGGGCTCGGGAACCGGGCATCCATCCC 60.2 0.06
mm4_intron_Lrrc7_chr3_158328323_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Lrrc7_chr3_158328323_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Kntc1_chr5_123753019_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Kntc1_chr5_123753019_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Epha3|Csnka2ip_chr16_64164363_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Epha3|Csnka2ip_chr16_64164363_R Primer3: not found at this Tm N.d. 0.06
mm4_exon_Cox5a_chr9_57521386_F TCGTCGGCAGCGTCCTGCCCTCCGTCGCTGTA 61.4 0.06
mm4_exon_Cox5a_chr9_57521386_R GTCTCGTGGGCTCGGCCAGGTCACAGCAAGGACAC 60.8 0.06
mm4_intergenic_Gm10800|Gm13809_chr2_98925531_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm10800|Gm13809_chr2_98925531_R Primer3: not found at this Tm N.d. 0.06
mm3_exon_4732444A12Rik/Notum_chr11_120660396_F TCGTCGGCAGCGTCCCTCCCATGGCAATGTCACT 60.0 0.06
mm3_exon_4732444A12Rik/Notum_chr11_120660396_R GTCTCGTGGGCTCGGCGAGGAGCAGCAGTGTCTT 59.7 0.06
mm4_intron_Acvr1_chr2_58521823_F TCGTCGGCAGCGTCGCTCCAGACTTGCACCAGAG 60.6 0.06
mm4_intron_Acvr1_chr2_58521823_R GTCTCGTGGGCTCGGGTGGCTACTCTGCAGGGAAG 60.1 0.06
mm4_intron_Nup107_chr10_117790734_F TCGTCGGCAGCGTCCTGAGTCCTGCTTACTCCCC 59.1 0.05
mm4_intron_Nup107_chr10_117790734_R GTCTCGTGGGCTCGGAGATAACTCCGCGGAGAACA 58.5 0.05
mm4_exon_Cul4a_chr8_13106130_F Primer3: not found at this Tm N.d. 0.05
mm4_exon_Cul4a_chr8_13106130_R Primer3: not found at this Tm N.d. 0.05
mm4_exon_Ppp2r1b_chr9_50880660_F TCGTCGGCAGCGTCAGTAAGTGTAGGAACCAGTGGC 59.6 0.05
mm4_exon_Ppp2r1b_chr9_50880660_R GTCTCGTGGGCTCGGTACTGCACGGTATCGCAACA 59.7 0.05
mm4_intergenic_Gm16104|Nfic_chr10_81419690_F TCGTCGGCAGCGTCGCCTCCCATAGTAACCCAGC 59.8 0.05
mm4_intergenic_Gm16104|Nfic_chr10_81419690_R GTCTCGTGGGCTCGGGGGTGAGACCATGGTCAAGG 60.0 0.05
mm4_intron_Arhgap24_chr5_102759323_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Arhgap24_chr5_102759323_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm15778|Ipo8_chr6_148740946_F TCGTCGGCAGCGTCGCGTGGCATTATGGGTCTTC 59.3 0.05
mm4_intergenic_Gm15778|Ipo8_chr6_148740946_R GTCTCGTGGGCTCGGTGTGTCCTGGAAGCTCCTTT 58.8 0.05
mm4_exon_Rxrb_chr17_34037571_F Primer3: not found at this Tm N.d. 0.05
mm4_exon_Rxrb_chr17_34037571_R Primer3: not found at this Tm N.d. 0.05
mm4_exon_Uhrf2_chr19_30030500_F Primer3: not found at this Tm N.d. 0.05
mm4_exon_Uhrf2_chr19_30030500_R Primer3: not found at this Tm N.d. 0.05
mm4_exon_Glp1r_chr17_30901857_F TCGTCGGCAGCGTCGCAGGTGGCAGCTATGACC 60.8 0.05
mm4_exon_Glp1r_chr17_30901857_R GTCTCGTGGGCTCGGAGGAGGCTTGGGGTGCTG 61.6 0.05
mm4_intron_Sned1_chr1_93243537_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Sned1_chr1_93243537_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Sept1|Zfp553_chr7_127232995_F TCGTCGGCAGCGTCTAGAACTTGGCAGGCTCCAG 59.3 0.04
mm4_intergenic_Sept1|Zfp553_chr7_127232995_R GTCTCGTGGGCTCGGATGGAGTGGCAGACAAAGGG 59.9 0.04
mm4_intron_Wfdc3_chr2_164738304_F TCGTCGGCAGCGTCCCCCTGCAAGCTACCAATGA 60.0 0.04
mm4_intron_Wfdc3_chr2_164738304_R GTCTCGTGGGCTCGGTGGCTTAGCTTTCTTGGACAGA 59.6 0.04
mm4_exon_Zer1_chr2_30105994_F Primer3: not found at this Tm N.d. 0.04
mm4_exon_Zer1_chr2_30105994_R Primer3: not found at this Tm N.d. 0.04
mm4_exon_Hfe2_chr3_96527307_F TCGTCGGCAGCGTCGGACCTGATGATCCAGCACA 59.4 0.04
mm4_exon_Hfe2_chr3_96527307_R GTCTCGTGGGCTCGGAAACCGGGCCTCATAGTCAC 59.7 0.04
mm4_intron_3110040N11Rik_chr7_81782788_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_3110040N11Rik_chr7_81782788_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Nemf_chr12_69356915_F TCGTCGGCAGCGTCAAGACCTCTCGGAATCAGCG 59.5 0.04
mm4_intron_Nemf_chr12_69356915_R GTCTCGTGGGCTCGGGGTGCCTCGGGGATTCTC 59.4 0.04
mm4_intron_Ptpn13_chr5_103580201_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Ptpn13_chr5_103580201_R Primer3: not found at this Tm N.d. 0.04
mm3_intron_Unc5a_chr13_54983694_F Primer3: not found at this Tm N.d. 0.04
mm3_intron_Unc5a_chr13_54983694_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Gm11186_chr11_53119661_F TCGTCGGCAGCGTCTCTCATCCCCACCCTCCATT 59.9 0.04
mm4_intron_Gm11186_chr11_53119661_R GTCTCGTGGGCTCGGTCAGGCTAGCCTCAACCTCT 59.9 0.04
mm4_intron_Nrf1_chr6_30073566_F TCGTCGGCAGCGTCAAAGCCATGGAGAGTGCACA 59.8 0.03
mm4_intron_Nrf1_chr6_30073566_R GTCTCGTGGGCTCGGTGAGCAAAAGAGAAGGGTTGT 57.7 0.03
mm4_intergenic_4930569F06Rik|Suds3_chr5_117064730_F TCGTCGGCAGCGTCCAGCAGAGACTAACAATTCCCC 58.7 0.03
mm4_intergenic_4930569F06Rik|Suds3_chr5_117064730_R GTCTCGTGGGCTCGGTGGCACGCCTTTCTCTTTGA 60.1 0.03
mm4_intergenic_Fxyd4|Gm4875_chr6_117990811_F TCGTCGGCAGCGTCCGCTTGTTTCCTTGGGTGTG 59.9 0.03
mm4_intergenic_Fxyd4|Gm4875_chr6_117990811_R GTCTCGTGGGCTCGGGATGTTCCCCACTGTTGCCT 60.2 0.03
mm4_intergenic_Gm22106|Ptp4a3_chr15_73746696_F TCGTCGGCAGCGTCCCCCTCCCTTTAAGGATGCC 59.8 0.03
mm4_intergenic_Gm22106|Ptp4a3_chr15_73746696_R GTCTCGTGGGCTCGGGGTGCAACCCCTCACAAAAC 59.8 0.03
mm4_exon_Osbpl3_chr6_50328773_F TCGTCGGCAGCGTCAGCAGAACTTCCTGGGCATC 60.0 0.03
mm4_exon_Osbpl3_chr6_50328773_R GTCTCGTGGGCTCGGCTGCCCTGCCACTAGGAAAA 59.9 0.03
mm4_exon_A230009B12Rik_chr17_10473344_F TCGTCGGCAGCGTCACACCAATGACCTCAGCGAG 60.0 0.03
mm4_exon_A230009B12Rik_chr17_10473344_R GTCTCGTGGGCTCGGCGAAGTCTCCTCTGGCATCC 59.8 0.03
mm4_intron_Ddr2_chr1_170065685_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Ddr2_chr1_170065685_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm12943|Dlgap3_chr4_127168449_F TCGTCGGCAGCGTCCTTCGGACAGTCCCCTTTCC 60.0 0.03
mm4_intergenic_Gm12943|Dlgap3_chr4_127168449_R GTCTCGTGGGCTCGGCCGCTATACCTGCCGTCTTA 59.0 0.03
mm4_intergenic_C2cd2l|Dpagt1_chr9_44322938_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_C2cd2l|Dpagt1_chr9_44322938_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Trappc9_chr15_72984514_F TCGTCGGCAGCGTCCTATAGCAGCAACGGTGGCT 60.1 0.02
mm4_intron_Trappc9_chr15_72984514_R GTCTCGTGGGCTCGGGAAGTCAGGAGTTGGGGTGG 59.9 0.02
mm4_exon_CT573030.1_chr17_34770615_F TCGTCGGCAGCGTCTCTTTCTTGGCCTCCACTGC 60.2 0.02
mm4_exon_CT573030.1_chr17_34770615_R GTCTCGTGGGCTCGGCTTCCTGCTGCTGGACTCTC 60.1 0.02
mm4_intergenic_Rybp|Gm23234_chr6_100422160_F TCGTCGGCAGCGTCCAGGAGGCAGAGACTTCCAG 59.4 0.02
mm4_intergenic_Rybp|Gm23234_chr6_100422160_R GTCTCGTGGGCTCGGCAGAGGTAGGGCCCTTGTCT 60.6 0.02
mm4_intron_1300002K09Rik_chr4_45867494_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_1300002K09Rik_chr4_45867494_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Gabbr2_chr4_46675502_F TCGTCGGCAGCGTCCTTCTGAGGTCGGGGCAAAG 60.6 0.02
mm4_intron_Gabbr2_chr4_46675502_R GTCTCGTGGGCTCGGTGAGCCTTAATCGACGTTCCA 59.4 0.02
mm4_intergenic_Plin2|Dennd4c_chr4_86724382_F TCGTCGGCAGCGTCGCATGCTTGCCTAGTTGC 57.2 0.02
mm4_intergenic_Plin2|Dennd4c_chr4_86724382_R GTCTCGTGGGCTCGGGTCTCACACTGTAGCCCAGG 59.7 0.02
mm4_exon_Lpcat4_chr2_112242306_F Primer3: not found at this Tm N.d. 0.02
mm4_exon_Lpcat4_chr2_112242306_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Anpep_chr7_79827239_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Anpep_chr7_79827239_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Sh3pxd2b_chr11_32357998_F TCGTCGGCAGCGTCTGCGGAAGTGCATCTGTGAT 60.0 0.02
mm4_intron_Sh3pxd2b_chr11_32357998_R GTCTCGTGGGCTCGGCCCTCACTGATAGCACTGGC 60.1 0.02
mm4_intergenic_Gm26228|Gdf10_chr14_33657511_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm26228|Gdf10_chr14_33657511_R Primer3: not found at this Tm N.d. 0.02
mm4_exon_Ric8_chr7_140861359_F TCGTCGGCAGCGTCTACACAGGCTACGGGAATGC 59.8 0.02
mm4_exon_Ric8_chr7_140861359_R GTCTCGTGGGCTCGGACATACCTGGCCTTGGCTTC 60.0 0.02
mm4_exon_Mmgt2_chr11_62648885_F Primer3: not found at this Tm N.d. 0.02
mm4_exon_Mmgt2_chr11_62648885_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Skint3_chr4_112267231_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Skint3_chr4_112267231_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm11493|Akap1_chr11_88821292_F TCGTCGGCAGCGTCGCACGAGAACAGAGAGGAGG 59.8 0.02
mm4_intergenic_Gm11493|Akap1_chr11_88821292_R GTCTCGTGGGCTCGGTTGGTGTCCCCATGAGAACG 59.9 0.02
mm4_intergenic_Gm23067|Gm26072_chr5_70193689_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm23067|Gm26072_chr5_70193689_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Ltbp1_chr17_75006096_F Primer3: not found at this Tm N.d. 0.01
mm4_exon_Ltbp1_chr17_75006096_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm9905/Mettl9|SNORA7_chr7_121035490_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm9905/Mettl9|SNORA7_chr7_121035490_R Primer3: not found at this Tm N.d. 0.01
mm3_exon_Ankrd63_chr2_118702825_F Primer3: not found at this Tm N.d. 0.01
mm3_exon_Ankrd63_chr2_118702825_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Tecpr1|2900089D17Rik_chr5_144230133_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Tecpr1|2900089D17Rik_chr5_144230133_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Gm26907_chr9_81852412_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Gm26907_chr9_81852412_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Fbxw4_chr19_45659697_F TCGTCGGCAGCGTCTTCAGTATTCCGGGTGCCAC 60.0 0.01
mm4_intron_Fbxw4_chr19_45659697_R GTCTCGTGGGCTCGGCTCGGCACCGACCTGTAA 59.0 0.01
mm4_intron_Ak7_chr12_105737528_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Ak7_chr12_105737528_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Npas3_chr12_54004006_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Npas3_chr12_54004006_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Trpc5_chrX_144391907_F TCGTCGGCAGCGTCAGGACCATGATAGTTTGTGATTGA 57.9 0.01
mm4_intron_Trpc5_chrX_144391907_R GTCTCGTGGGCTCGGAACGCAGGACCTGTAGAAGC 60.0 0.01
mm4_exon_Dmrta2_chr4_109981952_F TCGTCGGCAGCGTCGGTTCGGAGGCTGACAAAGA 59.9 0.01
mm4_exon_Dmrta2_chr4_109981952_R GTCTCGTGGGCTCGGAAGATGTCCAGCGGCGTC 60.1 0.01
mm4_intergenic_F630040K05Rik|Vegfa_chr17_45872110_F TCGTCGGCAGCGTCAGGAGGCCTCTGTAGTCAGG 60.0 0.01
mm4_intergenic_F630040K05Rik|Vegfa_chr17_45872110_R GTCTCGTGGGCTCGGACCCCGTCTCCTCAACAAAC 59.8 0.01
mm4_intergenic_9030622O22Rik|Foxa2_chr2_148040887_F TCGTCGGCAGCGTCACATAGCAGGTTAGGGCAGC 59.8 0.01
mm4_intergenic_9030622O22Rik|Foxa2_chr2_148040887_R GTCTCGTGGGCTCGGAGCCCAGCCTGAAATGTGTT 60.1 0.01
mm4_intergenic_Gm16391|Gm17538_chr17_76962079_F TCGTCGGCAGCGTCACCACTGGCATTCAAATGACTC 59.1 0.01
mm4_intergenic_Gm16391|Gm17538_chr17_76962079_R GTCTCGTGGGCTCGGTGTCCCTTGTTGCAAAATGACA 59.2 0.01
mm4_intergenic_Gm23041|Nav3_chr10_109454514_F TCGTCGGCAGCGTCGGATTCCATGCTAGGTCGCA 59.8 0.01
mm4_intergenic_Gm23041|Nav3_chr10_109454514_R GTCTCGTGGGCTCGGAGGCATCCATTCAAAGAGCA 57.4 0.01
mm4_exon_Sspo_chr6_48466331_F TCGTCGGCAGCGTCATCCACATGACCACTCCTGC 59.7 0.01
mm4_exon_Sspo_chr6_48466331_R GTCTCGTGGGCTCGGCGTGGGAGGCAGAACATAGG 60.1 0.01
mm4_exon_Tesk1_chr4_43443415_F Primer3: not found at this Tm N.d. 0.01
mm4_exon_Tesk1_chr4_43443415_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Mir1931|Cdk17_chr10_93190541_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Mir1931|Cdk17_chr10_93190541_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Rerg_chr6_137153290_F TCGTCGGCAGCGTCCACTGTTGAGTCACGTGCAC 59.6 0.01
mm4_intron_Rerg_chr6_137153290_R GTCTCGTGGGCTCGGTGTGCTGCTCCCTCAATGTT 59.8 0.01
mm4_intergenic_Gm8609|Gm22543_chr16_40006867_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm8609|Gm22543_chr16_40006867_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Numbl/Adck4_chr7_27258727_F TCGTCGGCAGCGTCGGAGGGCGGGGTTATCTTTT 59.7 0.00
mm4_exon_Numbl/Adck4_chr7_27258727_R GTCTCGTGGGCTCGGCGGGAGGATTCCGTTCCG 59.8 0.00
mm4_intron_Rere_chr4_150609618_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Rere_chr4_150609618_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm14822|Magee2_chrX_104774620_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm14822|Magee2_chrX_104774620_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Tmco6_chr18_36735369_F TCGTCGGCAGCGTCATGTGGCGGTTGGGAATAGG 60.1 0.00
mm4_exon_Tmco6_chr18_36735369_R GTCTCGTGGGCTCGGTGCTGACTAACTGCTGCTCC 60.0 0.00
mm4_intergenic_Mcm5|Rasd2_chr8_75147930_F TCGTCGGCAGCGTCCGTAGGGCTTGGAGACAGAC 59.8 0.00
mm4_intergenic_Mcm5|Rasd2_chr8_75147930_R GTCTCGTGGGCTCGGCCCTTCTGCATGCTTCTCCA 60.0 0.00
mm4_intergenic_Rnd1|Ccdc65_chr15_98701761_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Rnd1|Ccdc65_chr15_98701761_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm8325|P2ry1_chr3_61002646_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm8325|P2ry1_chr3_61002646_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm26469|Gm14258_chr2_169063657_F TCGTCGGCAGCGTCGAGGCCACAGATGTTGCTCT 60.0 0.00
mm4_intergenic_Gm26469|Gm14258_chr2_169063657_R GTCTCGTGGGCTCGGTGTGTTCCTCTGAGCGTGAC 59.9 0.00
mm4_intron_Zfat_chr15_68258383_F TCGTCGGCAGCGTCACTCGAACACCTATGCAGCC 60.1 0.00
mm4_intron_Zfat_chr15_68258383_R GTCTCGTGGGCTCGGAAAAGGGAGGCGGTGGAGAG 61.8 0.00
mm4_intron_Ushbp1_chr8_71391126_F TCGTCGGCAGCGTCACCTGACATCAAAGCCCCAG 59.9 0.00
mm4_intron_Ushbp1_chr8_71391126_R GTCTCGTGGGCTCGGCAACACTCCCCAAAGCAAAGA 58.9 0.00
mm4_intron_Tnrc6c_chr11_117724063_F TCGTCGGCAGCGTCTCGTAGACACCACCCAGTCA 60.1 0.00
mm4_intron_Tnrc6c_chr11_117724063_R GTCTCGTGGGCTCGGGTGTCCCAGGCTTCTTTCCT 59.5 0.00
mm4_intron_Pdik1l_chr4_134287415_F TCGTCGGCAGCGTCCTCTGGCCATTGCGCTCC 61.2 0.00
mm4_intron_Pdik1l_chr4_134287415_R GTCTCGTGGGCTCGGATTGGTCCGCTCTGCAGC 60.4 0.00
mm4_intron_Pcif1_chr2_164879798_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Pcif1_chr2_164879798_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Nox4_chr7_87246622_F TCGTCGGCAGCGTCCACAACAACAGGCTCGCG 59.7 0.00
mm4_intron_Nox4_chr7_87246622_R GTCTCGTGGGCTCGGGCTGTACTGAGTCTGGAGGC 59.8 0.00
mm4_intron_Golga1_chr2_39065333_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Golga1_chr2_39065333_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Gm14216_chr2_154731634_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Gm14216_chr2_154731634_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Emid1_chr11_5109678_F TCGTCGGCAGCGTCAAGCTAGGAGCCTGATGGGA 60.0 0.00
mm4_intron_Emid1_chr11_5109678_R GTCTCGTGGGCTCGGGCCTGATTGACACTGGTCCA 59.9 0.00
mm4_intron_E2f1_chr2_154568457_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_E2f1_chr2_154568457_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Crocc_chr4_141017352_F TCGTCGGCAGCGTCGCACCTCTGCCTAGCATCAT 59.8 0.00
mm4_intron_Crocc_chr4_141017352_R GTCTCGTGGGCTCGGCCTTAGGCCAGAGTCCTCCT 60.0 0.00
mm4_intron_Cdh22_chr2_165234344_F TCGTCGGCAGCGTCGAGCTCAGGACACAGGGAC 59.4 0.00
mm4_intron_Cdh22_chr2_165234344_R GTCTCGTGGGCTCGGGGACTTTCCCACGTCCAAAC 59.0 0.00
mm4_intron_Camkk2_chr5_122769337_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Camkk2_chr5_122769337_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Ap3b1_chr13_94540433_F TCGTCGGCAGCGTCCCATTCTAAATGCTCCCTGACC 58.7 0.00
mm4_intron_Ap3b1_chr13_94540433_R GTCTCGTGGGCTCGGAAATGTCATTATGCCCGCCA 58.2 0.00
mm4_intergenic_Swap70|Sbf2_chr7_110288811_F TCGTCGGCAGCGTCTGGGTCCAATGCTCTGTGTC 59.9 0.00
mm4_intergenic_Swap70|Sbf2_chr7_110288811_R GTCTCGTGGGCTCGGCTCCAAGTCCACAGAGGCTG 60.0 0.00
mm4_intergenic_Serinc2|Gm10570/Fabp3_chr4_130306905_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Serinc2|Gm10570/Fabp3_chr4_130306905_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Pianp|Zfp384_chr6_125004536_F TCGTCGGCAGCGTCCGTGGAAGGTGAAGAAGCCT 59.9 0.00
mm4_intergenic_Pianp|Zfp384_chr6_125004536_R GTCTCGTGGGCTCGGACGAGAGCATCTTGCCCTTC 60.1 0.00
mm4_intergenic_Nfia|Nfia/E130114P18Rik_chr4_97774622_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Nfia|Nfia/E130114P18Rik_chr4_97774622_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Mapk4|Mapk4/Gm9925_chr18_74064990_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Mapk4|Mapk4/Gm9925_chr18_74064990_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Lmod3|Frmd4b_chr6_97254366_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Lmod3|Frmd4b_chr6_97254366_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25651|4933400A11Rik_chrX_169766595_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25651|4933400A11Rik_chrX_169766595_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm24720|Tshz1_chr18_83371100_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm24720|Tshz1_chr18_83371100_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm24382|Mbnl1_chr3_60501057_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm24382|Mbnl1_chr3_60501057_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23856|Mst1r_chr9_107906821_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23856|Mst1r_chr9_107906821_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm12210|Gm11187_chr11_52545542_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm12210|Gm11187_chr11_52545542_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm10782|Slc25a48_chr13_56412746_F TCGTCGGCAGCGTCGACGGGCTTGAGTGGTTACA 59.9 0.00
mm4_intergenic_Gm10782|Slc25a48_chr13_56412746_R GTCTCGTGGGCTCGGAGCACTCAGGGAGGAGAGAG 60.0 0.00
mm4_exon_Tjp3_chr10_81281248_F TCGTCGGCAGCGTCCCAGACCGTTGGCTTCAGAT 60.0 0.00
mm4_exon_Tjp3_chr10_81281248_R GTCTCGTGGGCTCGGCAGGTCGATCATGGGGTGAG 59.8 0.00
mm4_exon_Sdk1_chr5_141241963_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Sdk1_chr5_141241963_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Nfe2_chr15_103255299_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Nfe2_chr15_103255299_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Ncam1_chr9_49509738_F TCGTCGGCAGCGTCTGCCCTCCTCCATGTCTTTG 59.6 0.00
mm4_exon_Ncam1_chr9_49509738_R GTCTCGTGGGCTCGGTGGTCATGGACATCACCTGC 60.0 0.00
mm4_exon_Mroh7_chr4_106727950_F TCGTCGGCAGCGTCAGGCTGACCCTACCCTTGAT 59.9 0.00
mm4_exon_Mroh7_chr4_106727950_R GTCTCGTGGGCTCGGGGGACTTCCGGATCATGGTG 60.1 0.00
mm4_exon_Faxc_chr4_21931726_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Faxc_chr4_21931726_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Cat_chr2_103467090_F TCGTCGGCAGCGTCGCCTGCCTCAGAACAAGTCT 59.9 0.00
mm4_exon_Cat_chr2_103467090_R GTCTCGTGGGCTCGGCAGATGGCTTTTGACCCAAGC 60.0 0.00
mm4_exon_2810428I15Rik_chr8_70504372_F TCGTCGGCAGCGTCTGATGGGAAAGCTGCAGAGG 60.0 0.00
mm4_exon_2810428I15Rik_chr8_70504372_R GTCTCGTGGGCTCGGCAGAGGAAGCCGGGAAAAGA 59.6 0.00
mm3_intergenic_4933434M16Rik|2610035D17Rik_chr11_112843190_F Primer3: not found at this Tm N.d. 0.00
mm3_intergenic_4933434M16Rik|2610035D17Rik_chr11_112843190_R Primer3: not found at this Tm N.d. 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

mm4_intron_Vstm2l_chr2_157921742 GAGTGTGTAGGCAGTCCCTGGTCCTGAACCTGACAGTGCCCTGGCTCGGTGCCCTGGCTTGTGAATCTGTCATGGGGGAG
GGAATGCCAGGGTACTGGGGGTGCTGTGTTCTGCCTGAAAGAACCCACAGG
mm4_intergenic_Agap1|Gm25180_chr1_89462071 TGCAGGTCAGTGTTAGCCACAGCTGGCCCTGCCTCCCTGGAGTTGAGACCCTCCTGCTCCTGTGCCAGCTGTCCTTGGCT
CAGCGTCCAGCCTGG
AGAGCTTAGATCTCCCAAGGGCAGATCTCAGGCCTTGCTCACA
mm4_intron_Itgb2l_chr16_96434098 TAAGTGCAAGTTCAGGGCCCCAGGGAGGATGGAGGGCTGAGAGCTGAGCCAGGGCAGAGTCAGCTTGCTGCAGCAGTGAC
ATTATAAAGAGAGCCTGGGACCGA
mm4_intergenic_Gm23926|Gm25115_chr14_86395723 CAGCTGGCGATCAGTCTTCTACAGCCCTGGCTCGGCTCCCCAGCCTGGACCGCTCCCAACTGCAGGGTCTGAAATGCCTC
T
mm4_exon_Srrm3_chr5_135857187 CAGAACGGAGGCAGCAGGCAGCGAAGCGGAGCGCACGGGGGTCGCCCCGGCTCGGCGCACAGCCCGCCCGATGTACGTAA
GCTTGGCTTTGTGGAGGGTTTGT
mm4_intron_Gpr133_chr5_129174821 TCAGAAGGAGATGGAGTGGGTACCATTCAGTCTGAGAACCCAGGCTCAGCGCCCTGCCTTGAGTTCCTAACTCCAAGTAC
TCAGCTGATGGTTCTGCT
mm4_intron_Vps13d_chr4_145193535 ACACAATGTGAAGCTGACTACCTTGTTTTATTATGTGAGCCTCAAACAGTCCTGGGCACCAAGCCAAGGCTAAAGTAATC
TTTATTTTTGTTATTGCCATTAATTTCAGCCCTAAAAGCCAACTCTCC
mm4_intron_Cecr5_chr6_120521901 CTCGGACCTCAGCATTGTGTTTTCACTTCTACGTAAAAACTGAACTTCCCTCACCTCTGCAGTGCTGGGCACTGAACCAT
GG
CCTTGCAATCGAGAAGGACTGGCT
mm3_intron_Ccz1_chr5_143998511 GAGAGCTCCCTTTCGGATCGTTGCTCAACAGGGCGACCCAGTGCCATCTTTATCTTTACAGAAGAGTGGCGAGGCCCAGC
GCTGTGAGCCGAGCCACGG
GCATCCTACCTTCCATAGTGCTGAAGATTCCCAGGC
mm4_intergenic_Gm12158|Ebf1_chr11_44823988 TCGTGGCAGAATTTCATAAGTGAGATATTTCAAAATATTTTAAGGCTTGGGACCGTGGCTCGGTGCACAGCACTTGCCTA
GAGTGTGTGAGACTTTG
mm4_intron_Cdh13_chr8_119288482 GCATGATCAGAGAGCAGCCTAACAACTGAGCAACTGGAATGGAAAGGTTCGAGGGTTAAGCATGGCCTGGCGCTGAGCCA
CGG
AGTCAGGGTTCTATGCTCGGTACACGGC
mm4_intergenic_Gm24365|Bag3_chr7_128514351 GCCCAGCCCTAACTAGGAGAACAGAGAGGAAGGGAAAACACAAGGGATGCTGTGGCCCAGGGAGACATCCTTGGCTCTGC
TCTCTGCCCTG
CCCTGCCTGACACCAGGGCCTCAGCTTTGCCCCACGGCTGTATAACACA
mm4_intron_Megf11_chr9_64485140 CAGCTGACTGGACTTGGAGGGGGAGATTGGATTCTGGCTGCTCTGCCTCTGGCCTGATTCTTCTGATCCCTGGCTGGGTG
CTCAGCTCTG
GTCCTCCTTGGAGGCCATGAAACAACCTTGTCAGCAGATTCTGTCCCCG
mm4_intergenic_Bicc1|Tfam_chr10_71224828 CCCACAGGAGGTAGTCAGGATTTGAACGCGCCCAGTCCAATTCTTAACCATTCCAAGATTTTCGGCTCTGCCAACCTTTG
GCAGAACTGGGGGCTGAGCCAAGGGCTTCAAGGATGTTACGCAGGCTCCTTATCCTAGC
mm4_intron_Tbx4_chr11_85912703 CAACACTGACCTGGGACCAGTGTGTCCAAGGCTCAGCTGCCAGCCCTGTGTTTTCCACAGACAGATTTGGGTTTGACTCA
TGTTACCTTGCCAAAGGTTCTTCGTGGGTCCTCAAGATCCT
mm4_exon_Dleu7/Rnaseh2b_chr14_62292782 GATGCTCCCGCGTCCCAGCCCCCTCCGGGTCCGCAGCTGGCCTGGGCGACGAGCCTGGGCGATGGGCACGTGGTCCGGGC
CGCGCGGGCTCCCTGGGGCGCTAGGACCGTCGCCCCAGCCCCACTCCTGCTGTAGTA
mm4_intergenic_Cbx6/Nptxr/Npcd|Apobec3_chr15_79848179 TCCGCCGATCATTCCATACCCGGCCCTGGTTCAGGGCCCAGCACTGGACTGTGGAGGCTGCCACTGTCACCATGCTGCAC
ACTGCCAGCTCTAGCCAGCATCCCAGTG
mm4_exon_Tcof1_chr18_60832425 GGCTGAGACCTCTGAGGACTCACCTTGGCTGGGCTCCCAGGCCCGTCACTGTCCAGCTCTGCACTGCTGCTCTCCGAGAC
ATCTTCTTTCTTTACCATTTGGACCCTGAGAGCTGC
mm4_intron_Sbf1_chr15_89313542 CTCACCACCATCCAAGTCCATTCCATTCCTCCAGCCCTGGCCCAGCGCCCAGCCCGAGACCCCAGCCAGCCCTCAGCCAG
CTTGGTTTCCTC
mm4_intergenic_Zfp428|Irgq_chr7_24529675 GGTCATTCCTGGCAGGTAGTCACTGCGGCCACCTGTAGCAGGGCGGGGCGTCGAGATATGGAGGTCCCTTTAGTGTCTGT
CACTCCTGCACCCAACCCCTCTTTCTGGCAGT
mm4_intergenic_Gm26674/Sash1|Gm9930_chr10_8916734 CGGAGGTGATCGCTTTGTTTTCTTCTGTTTTCCACATCTCATCAACAGTGCTGGGCATCGAGCAAAGGCTCAACAGGAGT
TTACTTCTAACAGCATAAAGGAGTTCAAAGGCATGCTATGCTGTCTGTACGCT
mm4_intron_Grik4_chr9_42720790 GTTTGGCCTGTTCTGCACTGATTCCCCAGATCCAAAGGCAGTGCCCATCCATTAAATACAATGTTTCTCAAAGAGTGTCC
GGACTACCTGCAGGGCTGGTTGCTGAGACACGGTGTTAAGTAGAGCGTAAAGATCCCGCC
mm4_intron_Sh3bp4_chr1_89125777 AGGAAGAGGGTGGTTTGTGCCAGACAAGGAAGTAAGGGCAGTGTGGCCACGCCAGGCAGACTGGCAGGCCGGGGCACCGA
GCCCTGG
GCTACACAAGTCGATACTACTTGTCAGTATCTGCA
mm4_intron_Tll2_chr19_41189268 TGAGGTCACAGGAACCGAGAGGTTGAAAGGGGGCAGAGAAGGAAGAGTAGACATGGTGAATGGAGATCCAGGGCTGGGAG
AAAAGCCAGGG
TGCAGAAGTTCTGAGGAGTCCTTCACTTTGGCACCGCATG
mm4_intergenic_Hbq1a|Sh3pxd2b_chr11_32307316 TCACAGTAACAAGGCAGGCCCAGAGGCTGTGCAACCCCGGGGCTGAGGGCTGAGCCATGGGTGGCTTCCTAGAGAATGGG
ACAAAAGAAATCATTTCCCAACTACCAAGAGGAAGCAGGTGGGTGAAG
mm4_intron_Fmo3_chr1_162957595 GTTGCCCACAGCTATGTCCTTCATACTTTACCATGGCTCTGATCCCAGCTCTGTTATGAGAGGATGCTGGCTGC
mm3_intergenic_Ttyh3|Iqce_chr5_140659731 AGGAAGTAGCCGAGTGGAGTGGTAGAAGCAGGAGGGTGGGGGCACACGAGGAGGAGGCGGGACTTCAGGTAGGTCAGCCC
TGGCTCAGCAGCCAGCCCTG
TCCGAGGGAAGCATCACATGTGCCAAGGGAGG
mm4_intron_Foxo3_chr10_42205329 TCCATTCCTTCCAGCCAAGCTTGCACTTGCGGCACACACAGATCCCTGGCAGCCTCGCACCTCTGCCCACACCCACGGCT
TGCCGCCCAGCCCTC
TTCCTCCCCTGCTCCCCAAGGCCTCACACTTCCCACTCCAC
mm4_intron_Adamts14_chr10_61261833 ACTCCCCTATGCCACTCCATCACCACCACAGCCCACCATGATCGGGCTGGGAGCTCTGCCTGGCCCTTTGCTTGGCCCCC
AGTCCTG
GCAGCGACTGGAGCTTCGGGACGAGGAGACAGGAAGTG
mm4_exon_Skiv2l/mmu-mir-6970_chr17_34845137 GCCTCCACTGCAGCATAGTACCTGAGCACAGGGTGGGTGGCGTGAGACCTCGCACAGGCCCAGTCTCTGCTCCCAGCCCT
G
AACTTACCCCTGGGTGTGGAAGGCCCCTCGGGAGTCCAGCAGCAGGAAGAG
mm4_intron_Msi2_chr11_88532488 CATAGACCTCGCACGTCTCCCTTCTTAGCGGCTTTAAAATAAGAAAAGAGCCAGAGCTGGGAGCCAAGGCAGGGACCTAA
ACGTCTGTCTTTCTGGCAGACTCCTCGAAGGAGACAAGGCCAGAGCTGTT
mm4_exon_Nr5a1_chr2_38692773 AGGGCACTTCTTGTCTTGGGGCTGTGGGTCTCTGGCCTTGGTGGGAGCACGCATTTCAGGGGCAGAGGTGGGGGCTGAGC
CAGGG
TAGTAGAAGACAGGAGACAGCCTTGTCCAGTTGGGTGGGCCTAGCATAA
mm4_intergenic_Gm22704|Gm14234_chr2_168399482 TGAATGTCGAGAGCCATGGGGGATCATTGGGTCCGGAAGGAGGGGCCAGATGGCCGCAGGGCTGGGCTCTGAGCATTGGG
CTCCCGCTGCCCCTCAAAGACCAAACAGGGAGCAGAGTTGTGG
mm4_intron_Ngf_chr3_102488717 TGCGAGGCTCACATTGTTCTTCCTTAATATAACATGGGTTTCTGAGGCACAATAGTCAGGGCTGGGAGCCCAGCTGAGGA
CACAGGACAGGAGATGGCGAAGCTGCAGTTTGCCACTTTGTCCCTGAGCACA
mm3_intergenic_Gm23702|Kirrel3_chr9_34977897 CACATGAGCAAGGGCAGTGCGTCTGTCCGTGGCTCGCCGCACAGCCCTCTTGGTCCCCCCTCACGCTCCTCCAGCTTCAT
AAGCTTCCTCTGATCTGTTTGCTTGCTGAATTAGTAAAACAGCCATCTCCCCTCAAAC
mm4_intergenic_RP23-387F1.3|E030018B13Rik_chr7_63902032 ATCCAGAGGAAACCAGTGCCCCCATCTGACCCACGGATGGGGGCTGAGCCAAGGCAGATCCAACCCTCAGCGCCCTGTCC
CTTCAGGCTCTGTAAGAGAAGCTACTTCAGAACTGGGTTGTAGCAGTTGGC
mm4_intergenic_Osmr|Lifr_chr15_6919705 TTAGAGAGACACGTGGCTGCCTCATTCCTGTGAACCAATCCTTTGAATTTCTCCATTTCTGTGTGTCTCCATGTCTCGCT
GCCCATCCCTG
CTTAGTTGGTGTTCACTATCTGACTCTGTTTCTCACGGTCCCA
mm4_exon_Sdk2_chr11_114066590 CACCTCCGGCTAGCAAACTTTGCGGGTCCCACGGCCCGGCGCGCAGCCCCGTGCGCCCATGTCTGCGCCAGCTGCGGGCA
GGGATGGATGCCCGGTTC
mm4_exon_Cox5a_chr9_57521386 CTGCCCTCCGTCGCTGTACCGCAGCCGCGGCTGCCCGAGGCCTCCTGCACCCCGCCTCGGCTCCCAGCCCCGCCGCCGGT
AAGGTCCGCGCCCGCCACAGCCCTCGCTCGCGTGTCCTTGCTGTGACCTGG
mm3_exon_4732444A12Rik/Notum_chr11_120660396 CCTCCCATGGCAATGTCACTCCGGCCTGTGGCCACCTGCGGAGAGCGGACGGCCGTGAGGCGTCAGCANNNNNNNNNNNN
NNNNNNNNN
NNNNNNNAAGACACTGCTGCTCCTCG
mm4_intron_Acvr1_chr2_58521823 GCTCCAGACTTGCACCAGAGCCCTGGGTTGAAGCCCAGCCCTGCCAACCGTCTGTCACGTGGTCTGACCCAACAGTTCAT
GCAGGCAAAGCCGGCATCGTGACTTCCCTGCAGAGTAGCCAC
mm4_intron_Nup107_chr10_117790734 CTGAGTCCTGCTTACTCCCCCAAATCCCACCCAGTGCTCGGCTACCAGCCCTGTCTTTGACTGCCTAGTATCACAATAGT
ATGTTCTCCGCGGAGTTATCT
mm4_exon_Ppp2r1b_chr9_50880660 AGTAAGTGTAGGAACCAGTGGCCCCAGGCTCTGCTCCCAGCACTGTTAGTGAGCATTGTGCTGTTGCGATACCGTGCAGT
A
mm4_intergenic_Gm16104|Nfic_chr10_81419690 GCCTCCCATAGTAACCCAGCCCTGGCCCTACACCCCCAAGGCCTTGCCCTAGTCCATGGCACCAGATGGTGACTATGGCC
TGACCCTTGACTGTGCGCCCAACCCTGACCTTGACCATGGTCTCACCC
mm4_intergenic_Gm15778|Ipo8_chr6_148740946 GCGTGGCATTATGGGTCTTCCAGTTTACATATTTGCATATGCCCGATAGTCTGCATTCTTCCTTTCTTCCTTGGCTGTGT
GCCAAGCCCTG
GAAGCTGAGCTATGGGAAGAAAGAGGGATAAAGGAGCTTCCAGGACACA
mm4_exon_Glp1r_chr17_30901857 GCAGGTGGCAGCTATGACCCAGTCCTGAGCGCCCAGCCATGGCCAGCACCCCAAGCCTCCT
mm4_intergenic_Sept1|Zfp553_chr7_127232995 TAGAACTTGGCAGGCTCCAGCTCGACCTGCCTGCCCCGGCCCAGCCCCCACTCCGAGCTGGGCTTTGTTTNNNNNNNNNN
NNNNNNNNNNNNNN
NNNNNNNNNTCTCTGTTTCCCTTTGTCTGCCACTCCAT
mm4_intron_Wfdc3_chr2_164738304 CCCCTGCAAGCTACCAATGATTTCCAGATGTGGTCCCAGGGCTGGGCACAGAGTCCTGGAAAGACTACAGTGTTCTAGAC
TCTCTTATGGTTAGTCTGTCCAAGAAAGCTAAGCCA
mm4_exon_Hfe2_chr3_96527307 GGACCTGATGATCCAGCACAACTGCTCACGCCAGGGTCCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNTGACCCCAGATCCCTGTGACTATGAGGCCCGGTTT
mm4_intron_Nemf_chr12_69356915 AAGACCTCTCGGAATCAGCGCCAGGGAGATGCAGCATCAGGCACGGTCCCCNNNNNNNNNNNNNNNNNNNNTGTCTCCCG
GGGGCACCGGCCCCGGGCTCGGCCCCCAGGCCTAGAGAATCCCCGAGGCACC
mm4_intron_Gm11186_chr11_53119661 TCTCATCCCCACCCTCCATTCCGCTTCCCAACATCATGTCACACCCACCTTCTGTCCTGTTGTAAAGCCCCAGGGCTGGG
TGTAGAGACACGG
GCCTCTTCAGATTGGAAGGATTAAGAGGTTGAGGCTAGCCTGA
mm4_intron_Nrf1_chr6_30073566 AAAGCCATGGAGAGTGCACACTGAATCTCCATGAACATGAATGAGGGGACTCTCAGTCTCCCTTTGCCCGGCTCCAAGCC
CTG
CTAACTATAAACAACCCTTCTCTTTTGCTCA
mm4_intergenic_4930569F06Rik|Suds3_chr5_117064730 CAGCAGAGACTAACAATTCCCCCGCCCCCGTTGCTCTGCCCCCAGCCCTTCAGCCAAATAAAAGTGCCTCTTAAATATCA
AGGGATCCTTCTACCTAAGACATTTGTGGCTCTTCAAAGAGAAAGGCGTGCCA
mm4_intergenic_Fxyd4|Gm4875_chr6_117990811 CGCTTGTTTCCTTGGGTGTGTGTTCAAGCAGTGGCTCAGGGCTGGGAGCTCATCCATGGCTTTTCCTTTCCTGTTTACCC
AGTGAAGATGCTGCAGTTCTTCCTGCCGCTCCCTCCTAGGCAACAGTGGGGAACATC
mm4_intergenic_Gm22106|Ptp4a3_chr15_73746696 CCCCTCCCTTTAAGGATGCCTTCCCTGGGCTGGGCACATAGCCAAGGGGGTTGGGGAGAGCCTGGGGTTAAGGAGCCTGT
TTTGTGAGGGGTTGCACC
mm4_exon_Osbpl3_chr6_50328773 AGCAGAACTTCCTGGGCATCGAAGAACTCAGAAAGGGAGTCGGTGATAGACAGCCTGCTCTCATTGGAGAGCTGGTGCAC
CAGGGCTCGGCCCTCATCCCTG
GAGTTTTCCTAGTGGCAGGGCAG
mm4_exon_A230009B12Rik_chr17_10473344 ACACCAATGACCTCAGCGAGCATGGCTGCCGTGATGGTGGTCCTATGGGGAACATTTGCCAACAACTGAGTGACTCAGGC
CTGGCTGCAGAGCCAAGG
TCCTCCAATAGGCATGTGGGCTGGATGCCAGAGGAGACTTCG
mm4_intergenic_Gm12943|Dlgap3_chr4_127168449 CTTCGGACAGTCCCCTTTCCCCCCTCTACCCCACCCCCGCCATGGCACAGCCCCTGGCTCGGGGCCCAGAGGTGGGCGGG
CACAGTGGGCACGGGTGGGGCCCCCAGCTAAGACGGCAGGTATAGCGG
mm4_intron_Trappc9_chr15_72984514 CTATAGCAGCAACGGTGGCTTTGACTTCTCCATCAACGCTGTAGGATGAGGAGAACATTTCTTTTCCCAGGTAGTTCAGA
ACCCATGGCCCGGCACTCAGCCTTGCTGGCACTAACCACCCCAACTCCTGACTTC
mm4_exon_CT573030.1_chr17_34770615 TCTTTCTTGGCCTCCACTGCAGCATAGTACCTGAGCACAGGGTGGGTGGCGTGAGACCTCGCACAGGCCCAGTCTCTGCC
CCCAGCCCTG
AACTTACTCCTGTGTGTGGAAGGCCCCTCGAGAGTCCAGCAGCAGGAAG
mm4_intergenic_Rybp|Gm23234_chr6_100422160 CAGGAGGCAGAGACTTCCAGGCTGCGAGTGGCTGAGCAGGGCTCCTTTCAGCCACTGTTCTTTGAAATTCTGCACAAAGC
CTTTTCTTTTCAGGGCTGGGTTCCAACCCAGGGCTAGACAAGGGCCCTACCTCTG
mm4_intron_Gabbr2_chr4_46675502 CTTCTGAGGTCGGGGCAAAGCTGCTGCCTACTAAGTTTCTACATGAATGAAAAAACAAACCACTACTCTTATTTGTGCTA
TGCAGTGGCAGGGCTGGGAGCCGAGGATTGGTCCAATGGAACGTCGATTAAGGCTCA
mm4_intergenic_Plin2|Dennd4c_chr4_86724382 GCATGCTTGCCTAGTTGCCCCTGGCTTGGCCCCCAGAACTGAATAAATTTGGGGTAGTGTTGAGGGTAGCCTGGGCTACA
GTGTGAGAC
mm4_intron_Sh3pxd2b_chr11_32357998 TGCGGAAGTGCATCTGTGATGCAGAGGGGGACAGGGCTGGGATCAGAGCCCTGGGCATTCGGTGAGGGAGGGCCAGATGG
GGATGGGTGAGCCAGTGCTATCAGTGAGGG
mm4_exon_Ric8_chr7_140861359 TACACAGGCTACGGGAATGCTGCCGGCCTCCTGGCTGCCAGGGGCCTCATGGCTGGGGGCCGACCCGAGGGCCAGTACTC
AGAGGACGAGGACACGGACACAGAGGAGTACAGGGAAGCCAAGGCCAGGTATGT
mm4_intergenic_Gm11493|Akap1_chr11_88821292 GCACGAGAACAGAGAGGAGGGGCCGCTGAGGGGTCCAGAGGCAAAAGGCAAGGTCCCCCTGCTCCCGGCGGGGTATCCTT
GCCTGGGCTCCCAGCCCAG
AGTAGACGTTCTCATGGGGACACCAA
mm4_intron_Fbxw4_chr19_45659697 TTCAGTATTCCGGGTGCCACACGGTCCCGAGCCGACCAGGCCTCGGCGCCCCGCCCTTTCCCAGGACGCTNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTACAGGTCGGTGCCGAG
mm4_intron_Trpc5_chrX_144391907 AGGACCATGATAGTTTGTGATTGATGATTTATTCCCATTAATTTTTATCTTAAAATGCTTTTAGATAAGAGTTCCTGTTG
CAGGGCTTGGGGCCGAGCTCAGGAAGAGGTACATGCTTAGCTTCTACAGGTCCTGCGTT
mm4_exon_Dmrta2_chr4_109981952 GGTTCGGAGGCTGACAAAGAAGAAGCCGAGGCCGCACCGACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCGGAC
GCCGCTGGACATCTT
mm4_intergenic_F630040K05Rik|Vegfa_chr17_45872110 AGGAGGCCTCTGTAGTCAGGCAGAGGACAGACAGGGCTGGGGGCCAAGAGAGGGTAGTGCCAACTGAAATGGCAACACAC
TCCCTGACCCTTACTAGGTGTCCTGTTTCCTTAGTTTGTTGAGGAGACGGGGT
mm4_intergenic_9030622O22Rik|Foxa2_chr2_148040887 ACATAGCAGGTTAGGGCAGCGCTTTCAGTCTCCATTGTCCCCCCCACTCGGCCCCCAGCCCTGCCTCTCCGCTCCTTCGC
CACAGGAGCCTTTTATGTGATAATGAAACACATTTCAGGCTGGGCT
mm4_intergenic_Gm16391|Gm17538_chr17_76962079 ACCACTGGCATTCAAATGACTCTCACTTGGCTTCCCTTGCTAGGCAACCAGCCCTGCTATGCATCAACATTACTTGCCAT
TGTCATTTTGCAACAAGGGACA
mm4_intergenic_Gm23041|Nav3_chr10_109454514 GGATTCCATGCTAGGTCGCATCTACCCAAAGCCATGTGGCAGGAAGGAATGACATGGTTACTAAAACACAGCATCTTCAG
CAGGCCCTGCCTGGGAGCCCATCCCTGCTGATTTTTTGCTCTTTGAATGGATGCCT
mm4_exon_Sspo_chr6_48466331 ATCCACATGACCACTCCTGCCCCTGCCCCTGCCTCTGCCCCCAGCCCTCAGATTGGAGCCCATCTAGTCACCTATGTTCT
GCCTCCCACG
mm4_intron_Rerg_chr6_137153290 CACTGTTGAGTCACGTGCACCCTCAGCAGTGCTGGGCGCCCAGCACAGGCAGCTCTCTAATGCACAGATATGCCTGCTTA
GTTTTAAGTCGAATTTTTTTTTCTCTCTAAACGGAAATTGAACATTGAGGGAGCAGCACA
mm4_exon_Numbl/Adck4_chr7_27258727 GGAGGGCGGGGTTATCTTTTGCATATACAGCAGGCGGCGGGCCAATGGGCGGGGTCGGGGCGGGGCGCCGAGGGAGGGAT
GCTCCCCCGCCCCCGGAACGGAATCCTCCCG
mm4_exon_Tmco6_chr18_36735369 ATGTGGCGGTTGGGAATAGGGAAGAGGCTCCTGGCTCTGAGCCGAGTGTGTGCTCCCGAGCTTCTCATCCTTAACCGAGC
CGTTCCTCTGCCCCCAGCCCTG
CGGAAGGCGCGGAGGGAGCAGCAGTTAGTCAGCA
mm4_intergenic_Mcm5|Rasd2_chr8_75147930 CGTAGGGCTTGGAGACAGACCTGAGCCAGGGCAGGGGCCTCAGGGCTGGGAGGCTGGCCAAGGCCTTTTGGAGAAGCATG
CAGAAGGG
mm4_intergenic_Gm26469|Gm14258_chr2_169063657 GAGGCCACAGATGTTGCTCTTGCTTGTGCACTGCAGAGGGAGCTGCCCTCCCCCCTCCCCCGGCCCCCAGCCCTGTTTGC
TGTACTGAGTTTGCTGGTCACGCTCAGAGGAACACA
mm4_intron_Zfat_chr15_68258383 ACTCGAACACCTATGCAGCCAGACGCCCGCGGATGGCCGCTGGCGGGCGCCCTGGCACCGCCCACAGCCCTGGAAACTGC
TCCCCAGGCGCGCAGGGCGCTCCCCTCGCTTGCTCTCCACCGCCTCCCTTTT
mm4_intron_Ushbp1_chr8_71391126 ACCTGACATCAAAGCCCCAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTGCATCTTTGCTTTGGGGAGTGTTG
mm4_intron_Tnrc6c_chr11_117724063 TCGTAGACACCACCCAGTCAAGTACAAGCAAGCTCATTCTTCTGCCCTGTGCTGTGCATACAGTCTGGGGCTGCCCATGC
CATAGTGCATGCAGCCTTTGCATGGCGCCCAGCACTGGTGAGGAAAGAAGCCTGGGACAC
mm4_intron_Pdik1l_chr4_134287415 CTCTGGCCATTGCGCTCCGTCGCTGCCCTGGCGCGGCGCACAGCCGAGCGTTTCCAGCGGTGTGCGCGCTGCAGAGCGGA
CCAAT
mm4_intron_Nox4_chr7_87246622 CACAACAACAGGCTCGCGCGAGACAAAGGGGCTGGCGCAGGGCGGCGCGCGGGGCCAGGGGGCGTGGGGAGGCAAGGGAG
CAGCGCGGTGGGAGCCGGGTGGAGACCTCCAGCCAGGCCTCCAGACTCAGTACAGC
mm4_intron_Emid1_chr11_5109678 AAGCTAGGAGCCTGATGGGAGATCGAGAAGAGCAGACGGTGCGAGTCTGGCCTCTAAGGGCTGGGCTGGGAGCCAAGGGG
GGTGAAGGCGGCTACTCCAGGACAGGAATCTGGACCAGTGTCAATCAGGC
mm4_intron_Crocc_chr4_141017352 GCACCTCTGCCTAGCATCATTCCACAGAGCAAGACCCCTGTGTCCGCTCCCAGCCCTGCCATTACTGGGCTGTCTAGGAG
TCCTGGCACTATGAGAGGAGGACTCTGGCCTAAGG
mm4_intron_Cdh22_chr2_165234344 GAGCTCAGGACACAGGGACCGCGACTCCCGGGCCGGGAGGTGGCCGGGCTGGGCTCCGCGCCGGGGAAAGTTTGGACGTG
GGAAAGTCC
mm4_intron_Ap3b1_chr13_94540433 CCATTCTAAATGCTCCCTGACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNTGGCGGGCATAATGACATTT
mm4_intergenic_Swap70|Sbf2_chr7_110288811 TGGGTCCAATGCTCTGTGTCTGCAGGGAGTTGTCTTGCTCCAAATATAGCCTCCATGGGTGGGCGGGGAGCCAGGGATGC
TTCTCAGGCAGTGACTTCACTGGGCCCAGGGCCCAGCCTCTGTGGACTTGGAG
mm4_intergenic_Pianp|Zfp384_chr6_125004536 CGTGGAAGGTGAAGAAGCCTCTTTAACCTTTCCAGCGTTCCCCACAGGGCTGGAGGCCTTGCCATGGCGATGGGGAATGA
AAGAGCACTTGGAGTAGAAGGGCAAGATGCTCTCGT
mm4_intergenic_Gm10782|Slc25a48_chr13_56412746 GACGGGCTTGAGTGGTTACATGTTGTTGAAAGTCAGTGATGGCCGGCAGGGGTGGGCACGGAGCCCAGGGAAGTGGGACC
TCTCTCCTCCCTGAGTGCT
mm4_exon_Tjp3_chr10_81281248 CCAGACCGTTGGCTTCAGATCCGCCACCCGAACTCCGGCGCCCATGGCTGCCTACCCTGCCGCGGCGACCTGCCCTGGAC
CGGCGAGAACGCTCACCCCATGATCGACCTG
mm4_exon_Ncam1_chr9_49509738 TGCCCTCCTCCATGTCTTTGCCCTTGGCTCCGGGCCCAGCTTTGCCACACAGGTTAACAGCGATGCACATGAGCAGGCCA
CACTTGTTCAGGAAGTAGCAGGTGATGTCCATGACCA
mm4_exon_Mroh7_chr4_106727950 AGGCTGACCCTACCCTTGATACTGTCCCAAAACCACCCTTCCGCTTACCTGGGCTCCCCGCCCAGCCCAGGCCGGGCTTA
TAGTCTTTGTGAGGTCACCATGATCCGGAAGTCCC
mm4_exon_Cat_chr2_103467090 GCCTGCCTCAGAACAAGTCTTCTTCCTGGCTTACCTGAAGCATTTTGTCAGGGCTGGGCTCGATGCCAGGGGGCATATTG
CTTGGGTCAAAAGCCATCTG
mm4_exon_2810428I15Rik_chr8_70504372 TGATGGGAAAGCTGCAGAGGGAGAATTCTTTATTGCGTGAGGCACATTGGAGGGCAGCCCAGCAGGGCACGACCTCAGCC
AGCTGGCGGTCAGGGCTGGGCTCGACGCCATGGGCCTCTTTTCCCGGCTTCCTCTG

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_mN1FkRligAThyqKv3eg3.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.