CRISPOR

DNA break repair outcome predictions

Guide: GGGATTAGGAGCTTACTTGG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GCCAAGATCCCAGGGAGCCTGGGATTAGGAGCTTACTTGGGGGTTTTCCCCCTCCCCCCCTCTGGAGAGTCCGGGGATGG`	(Wild-type sequence)
362	`CAGGGAGCCTGGGATTAGGAGCTTACTTGG--GTTTTCCCCCTCCCCCCCTCTGGAGAGT`	`2 bp deleted → frameshift`
344	`CAGGGAGCCTGGGATTAGGAGCTTACTTGG---TTTTCCCCCTCCCCCCCTCTGGAGAGT`	`3 bp deleted → no frameshift`
220	`CAGGGAGCCTGGG--------------------TTTTCCCCCTCCCCCCCTCTGGAGAGT`	`20 bp deleted → frameshift`
208	`CAGGGAGCCTGGGATTAGG-------------GTTTTCCCCCTCCCCCCCTCTGGAGAGT`	`13 bp deleted → frameshift`
198	`CAGGGAGCCTGGGATTAGG--------------TTTTCCCCCTCCCCCCCTCTGGAGAGT`	`14 bp deleted → frameshift`
154	`CAGGGAGCCTGGG-------------------GTTTTCCCCCTCCCCCCCTCTGGAGAGT`	`19 bp deleted → frameshift`
148	`CAGGG----------------------------TTTTCCCCCTCCCCCCCTCTGGAGAGT`	`28 bp deleted → frameshift`
143	`CAGGGAGCCTGGGATTAGGAGCTTACTTGG-------------------------AGAGT`	`25 bp deleted → frameshift`
141	`CAGGGAGCCTGGGATTAGGAGCTTACTT-------TTCCCCCTCCCCCCCTCTGGAGAGT`	`7 bp deleted → frameshift`
140	`CAGGGAGCCTGG---------------------TTTTCCCCCTCCCCCCCTCTGGAGAGT`	`21 bp deleted → no frameshift`
134	`CAGGGAGCCTGGGATTAGGAGCTTACT----------------CCCCCCCTCTGGAGAGT`	`16 bp deleted → frameshift`
134	`CAGGGAGCCTGGGATTAGGAGCTTACTT--------TCCCCCTCCCCCCCTCTGGAGAGT`	`8 bp deleted → frameshift`
127	`CAGGGAGCCTGGGATTAGGAGCTTACTT---------CCCCCTCCCCCCCTCTGGAGAGT`	`9 bp deleted → no frameshift`
115	`CAGGGAGCCTGGGATTAGGAG------------------------------------AGT`	`36 bp deleted → no frameshift`
115	`CAGGGAGCCTGGGATTAGGAGCTT-----------TTCCCCCTCCCCCCCTCTGGAGAGT`	`11 bp deleted → frameshift`
110	`CAGGGAGCCTGGGATTAGGAGCT--------------------CCCCCCCTCTGGAGAGT`	`20 bp deleted → frameshift`
109	`CAGGGAGCCTGGGATTAGGAGCTT------------TCCCCCTCCCCCCCTCTGGAGAGT`	`12 bp deleted → no frameshift`
104	`CAGGGAGCCTGGGATTAGGAGCTT-------------CCCCCTCCCCCCCTCTGGAGAGT`	`13 bp deleted → frameshift`
103	`CAGGG---------------------------GTTTTCCCCCTCCCCCCCTCTGGAGAGT`	`27 bp deleted → no frameshift`
96	`CAGGGAGCCT---------------------------------CCCCCCCTCTGGAGAGT`	`33 bp deleted → no frameshift`
94	`CAGG-----------------------------TTTTCCCCCTCCCCCCCTCTGGAGAGT`	`29 bp deleted → frameshift`
90	`CAGGGAGCCTGGGATTAGGAGCTTACT------------------------CTGGAGAGT`	`24 bp deleted → no frameshift`
89	`CAGGGAGCC------------------------------CCCTCCCCCCCTCTGGAGAGT`	`30 bp deleted → no frameshift`
84	`CAGGGAGCC-------------------------------CCTCCCCCCCTCTGGAGAGT`	`31 bp deleted → frameshift`
81	`CAGGGAGCCTGGGATTAGGAGCTTACT--------------------------GGAGAGT`	`26 bp deleted → frameshift`
81	`CAGGGAGCCTGG-------------------------------------------AGAGT`	`43 bp deleted → frameshift`
80	`CAGGGAGCC--------------------------------CTCCCCCCCTCTGGAGAGT`	`32 bp deleted → frameshift`
77	`CAGGGAGCCTGGGATT-------------------TTCCCCCTCCCCCCCTCTGGAGAGT`	`19 bp deleted → frameshift`
75	`CAGGGAGCCTGGGATTAGGAG--------------------------------------T`	`38 bp deleted → frameshift`
74	`CAGGGAGCCTGGGATTAGGAGCT----------------------------CTGGAGAGT`	`28 bp deleted → frameshift`
73	`CAGGGAGCCTGGGATT--------------------TCCCCCTCCCCCCCTCTGGAGAGT`	`20 bp deleted → frameshift`
70	`CAGGGAGCCTGGGATT---------------------CCCCCTCCCCCCCTCTGGAGAGT`	`21 bp deleted → no frameshift`
66	`CAGGGAGCCTGGGATTAGGAGCT------------------------------GGAGAGT`	`30 bp deleted → no frameshift`
66	`CAGGGAGCC------------------------------------CCCCCTCTGGAGAGT`	`36 bp deleted → no frameshift`
64	`CAGGGAGCCT-----------------------------------------CTGGAGAGT`	`41 bp deleted → frameshift`
62	`CAGGGAGCC-------------------------------------CCCCTCTGGAGAGT`	`37 bp deleted → frameshift`
61	`CAGGGAGCCTGGGA------------------------------------------GAGT`	`42 bp deleted → no frameshift`
60	`CAGGGAGCC--------------------------------------CCCTCTGGAGAGT`	`38 bp deleted → frameshift`
57	`CAGGGAG--------------------------------------------------AGT`	`50 bp deleted → frameshift`
56	`CAGGGAGCC---------------------------------------CCTCTGGAGAGT`	`39 bp deleted → no frameshift`
54	`CAGGGAGCC----------------------------------------CTCTGGAGAGT`	`40 bp deleted → frameshift`
42	`CAGGGAGCCTGGGATTAG---------------------------------------AGT`	`39 bp deleted → no frameshift`
38	`CAGGGAGCCTGGGATTAG-----------------------------------------T`	`41 bp deleted → frameshift`
37	`CAGGGAG----------------------------------------------------T`	`52 bp deleted → frameshift`
33	`CAGGGAGCCTGGGA--------------------------------------------GT`	`44 bp deleted → frameshift`
31	`CAGG---------------------------------------------------AGAGT`	`51 bp deleted → no frameshift`
20	`CAG------------------------------------------------------AGT`	`54 bp deleted → no frameshift`
18	`CAG--------------------------------------------------------T`	`56 bp deleted → frameshift`

Version 5.01 - Documentation - Contact us - Downloads/local installation - Citation - License