CRISPOR

DNA break repair outcome predictions

Guide: TAGTAGCTCAGACACCGAAT CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CTCTCTGGCCAAGGGCAACCTAGTAGCTCAGACACCGAATCGGATTTCTATGAAGAAATCGAGGTGAGCTGCACCCCAGA`	(Wild-type sequence)
311	`AAGGGCAACCTAGTAGCTCAGACACCG-----GATTTCTATGAAGAAATCGAGGTGAGCT`	`5 bp deleted → frameshift`
222	`AAGGGCAACCTAGTAGCTCAGACACCGA------TTTCTATGAAGAAATCGAGGTGAGCT`	`6 bp deleted → no frameshift`
188	`AAGGGCAACCTAGTAGCTCAGACACCGAA---------------GAAATCGAGGTGAGCT`	`15 bp deleted → no frameshift`
164	`AAGGGCAACCTAGTAGCTCAGA------------TTTCTATGAAGAAATCGAGGTGAGCT`	`12 bp deleted → no frameshift`
162	`AAGGGCAACCTAGTAGCTCAGACACCGAA------------------ATCGAGGTGAGCT`	`18 bp deleted → no frameshift`
155	`AAGGGCAACCTAGTAGCTCAGACACCGAAT-----TTCTATGAAGAAATCGAGGTGAGCT`	`5 bp deleted → frameshift`
150	`AAGGGCAACCTAGTAGCTCAGACACCGA------------------------GGTGAGCT`	`24 bp deleted → no frameshift`
120	`AAGGGCAACCTAGTAGCTCAGA------------------------AATCGAGGTGAGCT`	`24 bp deleted → no frameshift`
116	`AAGGGCAACCTAGTAGCTCAGACACCGAAT-------------------CGAGGTGAGCT`	`19 bp deleted → frameshift`
116	`AAGGGCAACCTAGTAGCTC-------------------TATGAAGAAATCGAGGTGAGCT`	`19 bp deleted → frameshift`
115	`AAGGGCAACCTAGTAGCTCAGACACCGAAT-----------GAAGAAATCGAGGTGAGCT`	`11 bp deleted → frameshift`
105	`AAGGGCAACCTAGTAGCTCAGA---------------------AGAAATCGAGGTGAGCT`	`21 bp deleted → no frameshift`
105	`AAGGGCAACCTAGTAGCT---------------------ATGAAGAAATCGAGGTGAGCT`	`21 bp deleted → no frameshift`
98	`AAGGGCAACCTA----------------------------TGAAGAAATCGAGGTGAGCT`	`28 bp deleted → frameshift`
98	`AAGGG----------------------------ATTTCTATGAAGAAATCGAGGTGAGCT`	`28 bp deleted → frameshift`
94	`AAGG-----------------------------ATTTCTATGAAGAAATCGAGGTGAGCT`	`29 bp deleted → frameshift`
73	`AAGGGCAACCTAGTAGCT------------------------------------------`	`42 bp deleted → no frameshift`
70	`AAGGGCAACCTAGTAGCTCAGACACCGA-----------------------------GCT`	`29 bp deleted → frameshift`
70	`AAGGGCAACCTAGTAG-----------------------------AAATCGAGGTGAGCT`	`29 bp deleted → frameshift`
66	`AAGGGCAACCTAGTAGCTCAGA------------------------------GGTGAGCT`	`30 bp deleted → no frameshift`
63	`AAGGGCAACCTAGTAGCTC-------------------------------GAGGTGAGCT`	`31 bp deleted → frameshift`
60	`AAGGGCAACCTAGTAGCTCAG--------------------------------GTGAGCT`	`32 bp deleted → frameshift`
60	`AAGGGCAACCTAG--------------------------------AAATCGAGGTGAGCT`	`32 bp deleted → frameshift`
57	`AAGGGCAACCTAGTA-------------------------TGAAGAAATCGAGGTGAGCT`	`25 bp deleted → frameshift`
52	`AAGGGCAACCTAGTAGCTCAGA-----------------------------------GCT`	`35 bp deleted → frameshift`
48	`AAG------------------------------------------AAATCGAGGTGAGCT`	`42 bp deleted → no frameshift`
47	`AAGGGCAACCTAGTAGCTCAG-------------------------------------CT`	`37 bp deleted → frameshift`
47	`AAGGGCAACCTAGTAG-------------------------------------GTGAGCT`	`37 bp deleted → frameshift`
41	`AAGG--------------------------------------------------TGAGCT`	`50 bp deleted → frameshift`
40	`AAGGGCAACCTAG----------------------------------------GTGAGCT`	`40 bp deleted → frameshift`
38	`AAGGGCAACCTAGT-----------------------------------------GAGCT`	`41 bp deleted → frameshift`
34	`AAGGG-------------------------------------------------TGAGCT`	`49 bp deleted → frameshift`
33	`AAGGGCAA------------------------------------GAAATCGAGGTGAGCT`	`36 bp deleted → no frameshift`
31	`AAGGGCAACCTAG---------------------------------------------CT`	`45 bp deleted → no frameshift`
28	`AAGGGCAA---------------------------------------ATCGAGGTGAGCT`	`39 bp deleted → no frameshift`
28	`AAGGGC-----------------------------------------------------T`	`53 bp deleted → frameshift`
27	`AAGGGCAA----------------------------------------TCGAGGTGAGCT`	`40 bp deleted → frameshift`
25	`AAGGGCAACCT-------------------------------------------------`	`49 bp deleted → frameshift`
21	`AA---------------------------------------------ATCGAGGTGAGCT`	`45 bp deleted → no frameshift`
20	`AA----------------------------------------------TCGAGGTGAGCT`	`46 bp deleted → frameshift`
19	`AAG-------------------------------------------------------CT`	`55 bp deleted → frameshift`

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