CRISPOR

DNA break repair outcome predictions

Guide: CATCGACCGGCTCCTCCTCC TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TGCAGCACTGCCTGCTAGTCCATCGACCGGCTCCTCCTCCTGGCCTCCCCCGCCACGCTCCCGGGAAGTCGGAGGGCCGG`	(Wild-type sequence)
603	`CCTGCTAGTCCATCGACCGGCTCCTCCTCC--------CCCGCCACGCTCCCGGGAAGTC`	`8 bp deleted → frameshift`
519	`CCTGCTAGTCCATCGACCGGCTCCTCC-----------CCCGCCACGCTCCCGGGAAGTC`	`11 bp deleted → frameshift`
347	`CCTGCTAGTCCATCGACCGGCTCC--------------CCCGCCACGCTCCCGGGAAGTC`	`14 bp deleted → frameshift`
313	`CCTGCTAGTCCATCGACCGGC-------------CTCCCCCGCCACGCTCCCGGGAAGTC`	`13 bp deleted → frameshift`
311	`CCTGCTAGTCCATCGACCGGCTCCTCCTCC-----TCCCCCGCCACGCTCCCGGGAAGTC`	`5 bp deleted → frameshift`
255	`CCTGCTAGTCCATCGACCGGCTCCTCCTCC---------CCGCCACGCTCCCGGGAAGTC`	`9 bp deleted → no frameshift`
245	`CCTGCTAGTCCATCGACCGGCTCCTCCTCC---------------------CGGGAAGTC`	`21 bp deleted → no frameshift`
242	`CCTGCTAGTCCATCGACCGGCTCCTCCTCC----------CGCCACGCTCCCGGGAAGTC`	`10 bp deleted → frameshift`
233	`CCTGCTAGTCCATCGACCGGCTCC---------------------------CGGGAAGTC`	`27 bp deleted → no frameshift`
230	`CCTGCTAGTCCATCGACCGGCTCCTCCTCC-----------GCCACGCTCCCGGGAAGTC`	`11 bp deleted → frameshift`
219	`CCTGCTAGTCCATCGACCGGCTCCTCC------------CCGCCACGCTCCCGGGAAGTC`	`12 bp deleted → no frameshift`
210	`CCTGCTAGTCCATCGACCGGCTCCTCC------------------------CGGGAAGTC`	`24 bp deleted → no frameshift`
208	`CCTGCTAGTCCATCGACCGGCTCCTCC-------------CGCCACGCTCCCGGGAAGTC`	`13 bp deleted → frameshift`
198	`CCTGCTAGTCCATCGACCGGCTCCTCCTCC--------------ACGCTCCCGGGAAGTC`	`14 bp deleted → frameshift`
198	`CCTGCTAGTCCATCGACCGGCTCCTCC--------------GCCACGCTCCCGGGAAGTC`	`14 bp deleted → frameshift`
190	`CCTGCTAGTCCATCGACCG-----------------------CCACGCTCCCGGGAAGTC`	`23 bp deleted → frameshift`
188	`CCTGCTAGTCCATCGACCGGCTCC---------------CCGCCACGCTCCCGGGAAGTC`	`15 bp deleted → no frameshift`
179	`CCTGCTAGTCCATCGACCGGCTCC----------------CGCCACGCTCCCGGGAAGTC`	`16 bp deleted → frameshift`
170	`CCTGCTAGTCCATCGACCGGCTCCTCC-----------------ACGCTCCCGGGAAGTC`	`17 bp deleted → frameshift`
170	`CCTGCTAGTCCATCGACCGGCTCC-----------------GCCACGCTCCCGGGAAGTC`	`17 bp deleted → frameshift`
170	`CCTGCTAGTCCATCGACC-----------------TCCCCCGCCACGCTCCCGGGAAGTC`	`17 bp deleted → frameshift`
147	`CCTGCTAGTCCATCGACCGGCTCC--------------------ACGCTCCCGGGAAGTC`	`20 bp deleted → frameshift`
147	`CCTGCTAGTCCATCGACC--------------------CCCGCCACGCTCCCGGGAAGTC`	`20 bp deleted → frameshift`
146	`CCTGCTAGTCCATCGACCGG----------------------------------GAAGTC`	`34 bp deleted → frameshift`
140	`CCTGCTAGTCCATCGACC---------------------CCGCCACGCTCCCGGGAAGTC`	`21 bp deleted → no frameshift`
133	`CCTGCTAGTCCATCGACCGGCTCCTCCTCC----------------------GGGAAGTC`	`22 bp deleted → frameshift`
133	`CCTGCTAGTCCATCGACCGGC----------------------CACGCTCCCGGGAAGTC`	`22 bp deleted → frameshift`
133	`CCTGCTAGTCCATCGACC----------------------CGCCACGCTCCCGGGAAGTC`	`22 bp deleted → frameshift`
129	`CCTGCTAGTCC---------------------------CCCGCCACGCTCCCGGGAAGTC`	`27 bp deleted → no frameshift`
120	`CCTGCTAGTCC------------------------TCCCCCGCCACGCTCCCGGGAAGTC`	`24 bp deleted → no frameshift`
114	`CCTGCTAGTCCATCGACCGGCTCCTCC-------------------------GGGAAGTC`	`25 bp deleted → frameshift`
109	`CCTGCTAGTCCATCGACC--------------------------ACGCTCCCGGGAAGTC`	`26 bp deleted → frameshift`
103	`CCTGCTAGTCCATCG---------------------------CCACGCTCCCGGGAAGTC`	`27 bp deleted → no frameshift`
98	`CCTGCTAGTCCATCGACCGGCTCC----------------------------GGGAAGTC`	`28 bp deleted → frameshift`
98	`CCTGCTAGTCCATCGACCG----------------------------CTCCCGGGAAGTC`	`28 bp deleted → frameshift`
98	`CCTGCTAGTCC----------------------------CCGCCACGCTCCCGGGAAGTC`	`28 bp deleted → frameshift`
96	`CCTGCTAGTCCA---------------------------------CGCTCCCGGGAAGTC`	`33 bp deleted → no frameshift`
96	`CCT---------------------------------CCCCCGCCACGCTCCCGGGAAGTC`	`33 bp deleted → no frameshift`
95	`CCTGCTAGTCCATC-----------------------CCCCGCCACGCTCCCGGGAAGTC`	`23 bp deleted → frameshift`
94	`CCTGCTAGTCC-----------------------------CGCCACGCTCCCGGGAAGTC`	`29 bp deleted → frameshift`
94	`CCTGC-----------------------------CTCCCCCGCCACGCTCCCGGGAAGTC`	`29 bp deleted → frameshift`
89	`CCTGCTAGTCC------------------------------GCCACGCTCCCGGGAAGTC`	`30 bp deleted → no frameshift`
80	`CCTGCTAGTCCATCG--------------------------------CTCCCGGGAAGTC`	`32 bp deleted → frameshift`
76	`CCTGCTAGTCCATCGACC---------------------------------CGGGAAGTC`	`33 bp deleted → no frameshift`
74	`CCTG----------------------------GCCTCCCCCGCCACGCTCCCGGGAAGTC`	`28 bp deleted → frameshift`
70	`CCTGCTAGTCCATCGAC-----------------------------GCTCCCGGGAAGTC`	`29 bp deleted → frameshift`
69	`CCTGCTAGTCCATCGACCGG-----------------------------------AAGTC`	`35 bp deleted → frameshift`
67	`CCTGCTAGTCC----------------------------------------CGGGAAGTC`	`40 bp deleted → frameshift`
66	`CCTGCT------------------------------CCCCCGCCACGCTCCCGGGAAGTC`	`30 bp deleted → no frameshift`
66	`CC------------------------------------CCCGCCACGCTCCCGGGAAGTC`	`36 bp deleted → no frameshift`
63	`CCTGCTAGTCCATCGACCGGCTCCTCCTC-------------------------------`	`31 bp deleted → frameshift`
62	`CC-------------------------------------CCGCCACGCTCCCGGGAAGTC`	`37 bp deleted → frameshift`
60	`CCTGCTAGTCCATCG--------------------------------------GGAAGTC`	`38 bp deleted → frameshift`
60	`CCTGC--------------------------------------CACGCTCCCGGGAAGTC`	`38 bp deleted → frameshift`
60	`CC--------------------------------------CGCCACGCTCCCGGGAAGTC`	`38 bp deleted → frameshift`
58	`CCTGCT-------------------------------------------CCCGGGAAGTC`	`43 bp deleted → frameshift`
56	`CC---------------------------------------GCCACGCTCCCGGGAAGTC`	`39 bp deleted → no frameshift`
54	`CCTGCTAGTCCATCGACCGGCTCCTC----------------------------------`	`34 bp deleted → frameshift`
51	`CCTGCTAGTCC-----------------------------------------GGGAAGTC`	`41 bp deleted → frameshift`
49	`CCTGCTAGTCCATC------------------------------------CCGGGAAGTC`	`36 bp deleted → no frameshift`
49	`CCTGCTAGTC--------------------------------------------------`	`50 bp deleted → frameshift`
48	`CC------------------------------------------ACGCTCCCGGGAAGTC`	`42 bp deleted → no frameshift`
47	`CCTGCTAGTCCATCGACCGGCTC-------------------------------------`	`37 bp deleted → frameshift`
40	`CCTGCTAGTCCATCGA----------------------------------------AGTC`	`40 bp deleted → frameshift`
34	`CC-------------------------------------------------CGGGAAGTC`	`49 bp deleted → frameshift`
32	`CC--------------------------------------------------GGGAAGTC`	`50 bp deleted → frameshift`
30	`CCTGCTAGTCCATC----------------------------------------------`	`46 bp deleted → frameshift`
30	`CCT----------------------------------------------CCCGGGAAGTC`	`46 bp deleted → frameshift`

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