CRISPOR

DNA break repair outcome predictions

Guide: ACTGTAATGACGATTGCAGC AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CGAGTGGCGTCACCAGCGGTACTGTAATGACGATTGCAGCAGGAGGATGACAGCTTAGAAAGAAGAGGGCAATGGGGCTT`	(Wild-type sequence)
347	`CACCAGCGGTACTGTAATGACGATTGCAGC--------------TTAGAAAGAAGAGGGC`	`14 bp deleted → frameshift`
258	`CACCAGCGGTACTGTAATGACGATTGCAG---GAGGATGACAGCTTAGAAAGAAGAGGGC`	`3 bp deleted → no frameshift`
257	`CACCAGCGGTACTGTAATGAC--------------------AGCTTAGAAAGAAGAGGGC`	`20 bp deleted → frameshift`
222	`CACCAGCGGTACTGTAATGACGATTGCAG------GATGACAGCTTAGAAAGAAGAGGGC`	`6 bp deleted → no frameshift`
198	`CACCAGCGGTACTGTAATGACGAT--------------GACAGCTTAGAAAGAAGAGGGC`	`14 bp deleted → frameshift`
173	`CACCAGCGGTACTGTAATGACGA-----------GGATGACAGCTTAGAAAGAAGAGGGC`	`11 bp deleted → frameshift`
170	`CACCAGCGGTACTGTAATGACGATTGC-----------------TTAGAAAGAAGAGGGC`	`17 bp deleted → frameshift`
156	`CACCAGCGGTACTGTAATGACGATTG-------------ACAGCTTAGAAAGAAGAGGGC`	`13 bp deleted → frameshift`
149	`CACCAGCGGTACTGTAATGA--------------GGATGACAGCTTAGAAAGAAGAGGGC`	`14 bp deleted → frameshift`
128	`CACCAGCGGTACTGTAATGACGA-----------------CAGCTTAGAAAGAAGAGGGC`	`17 bp deleted → frameshift`
128	`CACCAGCGGTACTGTAATGA-----------------TGACAGCTTAGAAAGAAGAGGGC`	`17 bp deleted → frameshift`
120	`CACCAGCGG------------------------AGGATGACAGCTTAGAAAGAAGAGGGC`	`24 bp deleted → no frameshift`
116	`CACCAGCGGTACTGTAATGACGATTGCAG-------------------AAAGAAGAGGGC`	`19 bp deleted → frameshift`
109	`CACCAGC-------------------------------------TTAGAAAGAAGAGGGC`	`37 bp deleted → frameshift`
103	`CACCAGCGG---------------------------ATGACAGCTTAGAAAGAAGAGGGC`	`27 bp deleted → no frameshift`
95	`CACCAGCGGTACTGTAATGACGATTGCAG-----------------------AAGAGGGC`	`23 bp deleted → frameshift`
89	`CACCAGCGGTACTGTAATGACGATTGCAGC------------------------------`	`30 bp deleted → no frameshift`
86	`CACCAGCGGTACTG-------------------------ACAGCTTAGAAAGAAGAGGGC`	`25 bp deleted → frameshift`
81	`CACCAGCGGTACTGTAATGACGATTGCAG--------------------------AGGGC`	`26 bp deleted → frameshift`
81	`CACCAGCGGTACTGTAATGACGA--------------------------AAGAAGAGGGC`	`26 bp deleted → frameshift`
81	`CACCAG--------------------------GAGGATGACAGCTTAGAAAGAAGAGGGC`	`26 bp deleted → frameshift`
76	`CACCAGCGGTACTGTAATGACGATTGC---------------------------------`	`33 bp deleted → no frameshift`
74	`CACCAGCGGTACTGTAATGACGATTGCAG----------------------------GGC`	`28 bp deleted → frameshift`
70	`CACCAGCGGTACTGTAATGA-----------------------------AAGAAGAGGGC`	`29 bp deleted → frameshift`
70	`CACCAGCGGTAC-----------------------------AGCTTAGAAAGAAGAGGGC`	`29 bp deleted → frameshift`
70	`CACCAG-----------------------------GATGACAGCTTAGAAAGAAGAGGGC`	`29 bp deleted → frameshift`
70	`CACCAGCGGTACTGTAATGACGATT---------------------AGAAAGAAGAGGGC`	`21 bp deleted → no frameshift`
66	`CACCAGCGGTACTGTAATGACGA------------------------------AGAGGGC`	`30 bp deleted → no frameshift`
60	`CACCAGCGGTACT--------------------------------TAGAAAGAAGAGGGC`	`32 bp deleted → frameshift`
57	`CACCAGCGGTACTGTAATGACGA---------------------------------GGGC`	`33 bp deleted → no frameshift`
57	`CACCAGCGGTACTGTAATGA---------------------------------AGAGGGC`	`33 bp deleted → no frameshift`
49	`CACCAGCGGTACTGTAATGA------------------------------------GGGC`	`36 bp deleted → no frameshift`
45	`CAC--------------------------------------AGCTTAGAAAGAAGAGGGC`	`38 bp deleted → frameshift`
42	`CACCAGCGGTACTGTA-------------------------------GAAAGAAGAGGGC`	`31 bp deleted → frameshift`
40	`CA----------------------------------------GCTTAGAAAGAAGAGGGC`	`40 bp deleted → frameshift`
38	`CACCAGCGGTACTGTAA---------------------------------AGAAGAGGGC`	`33 bp deleted → no frameshift`
36	`CACCAGCGGTACTGTAA----------------------------------GAAGAGGGC`	`34 bp deleted → frameshift`
36	`CACCAG------------------------------------------AAAGAAGAGGGC`	`42 bp deleted → no frameshift`
34	`CACCAGCGG-------------------------------------------------GC`	`49 bp deleted → frameshift`
33	`CACCAGCGGTA------------------------------------GAAAGAAGAGGGC`	`36 bp deleted → no frameshift`
32	`CACCAGCGG--------------------------------------------------C`	`50 bp deleted → frameshift`
31	`CACCAGCGGTACTGTAA-------------------------------------GAGGGC`	`37 bp deleted → frameshift`
30	`CACCAG----------------------------------------------AAGAGGGC`	`46 bp deleted → frameshift`
28	`CACCAGC-----------------------------------------------------`	`53 bp deleted → frameshift`
25	`CACCAG-------------------------------------------------AGGGC`	`49 bp deleted → frameshift`
23	`CACCAG---------------------------------------------------GGC`	`51 bp deleted → no frameshift`

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