← return to the list of all guides
The following table lists possible deletions.
Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.
| mh- Score | Sequence | Effect |
|---|---|---|
| TCCCCACTGCCATCTCCGCGGTCGCCGTCGACACGGCGCTGGGGCTACCCGGCGCCTGCCTTGTCGCCTTAGCTCCTCTT | (Wild-type sequence) | |
| 538 | CATCTCCGCGGTCGCCGTCGACACGGCGC----------------CTGCCTTGTCGCCTT | 16 bp deleted → frameshift |
| 402 | CATCTCCGCGGTCGCCGTCGACACGGC--------TACCCGGCGCCTGCCTTGTCGCCTT | 8 bp deleted → frameshift |
| 370 | CATCTCCGCGGTCGCCGTCGACACGGCGCT------ACCCGGCGCCTGCCTTGTCGCCTT | 6 bp deleted → no frameshift |
| 296 | CATCTCCGCGGTCGCCGTCGACACGG------GGCTACCCGGCGCCTGCCTTGTCGCCTT | 6 bp deleted → no frameshift |
| 282 | CATCTCCGCGGTCGCCGTCGACACGG-------GCTACCCGGCGCCTGCCTTGTCGCCTT | 7 bp deleted → frameshift |
| 208 | CATCTCCGCGGTCGCCGTCGACACGGCG-------------GCGCCTGCCTTGTCGCCTT | 13 bp deleted → frameshift |
| 198 | CATCTCCGCGGTCGCCGTCGACACGGCGC--------------GCCTGCCTTGTCGCCTT | 14 bp deleted → frameshift |
| 180 | CATCTCCGCGGTCGCCG------------------------GCGCCTGCCTTGTCGCCTT | 24 bp deleted → no frameshift |
| 178 | CATCTCCGCGGTCGCC------------------------------TGCCTTGTCGCCTT | 30 bp deleted → no frameshift |
| 162 | CATCTCCGCGGTCGCCGTCGACACGGC------------------CTGCCTTGTCGCCTT | 18 bp deleted → no frameshift |
| 154 | CATCTCCGCGGTCGCCGTCGACACG-------------------CCTGCCTTGTCGCCTT | 19 bp deleted → frameshift |
| 149 | CATCTCCGCGGTCGCCGTCGACAC--------------CCGGCGCCTGCCTTGTCGCCTT | 14 bp deleted → frameshift |
| 148 | CATCTCCGCGGTCGCCGTCGACACGGCGC----------------------------CTT | 28 bp deleted → frameshift |
| 147 | CATCTCCGCGGTCGCCGTCGACACGGCGC--------------------CTTGTCGCCTT | 20 bp deleted → frameshift |
| 147 | CATCTCCGCGGTCGC--------------------TACCCGGCGCCTGCCTTGTCGCCTT | 20 bp deleted → frameshift |
| 140 | CATCTCCGCGGTCGCCGTCG---------------------GCGCCTGCCTTGTCGCCTT | 21 bp deleted → no frameshift |
| 140 | CATCTCCGCGG---------------------GGCTACCCGGCGCCTGCCTTGTCGCCTT | 21 bp deleted → no frameshift |
| 134 | CATCTCCGCGGTCGCCGTCGAC----------------CCGGCGCCTGCCTTGTCGCCTT | 16 bp deleted → frameshift |
| 134 | CATCTCCGCGGTCGCC------------------------------------------TT | 42 bp deleted → no frameshift |
| 133 | CATCTCCGCGGTCGCCGTCGACACGGC----------------------CTTGTCGCCTT | 22 bp deleted → frameshift |
| 133 | CATCTCCGCGG----------------------GCTACCCGGCGCCTGCCTTGTCGCCTT | 22 bp deleted → frameshift |
| 128 | CATCTCCGCGGTCGCCGTCGACACGGCGCT-----------------GCCTTGTCGCCTT | 17 bp deleted → frameshift |
| 127 | CATCTCCGCGG-------------------------------CGCCTGCCTTGTCGCCTT | 31 bp deleted → frameshift |
| 126 | CATCTCCGCGGTCGCC-----------------------CGGCGCCTGCCTTGTCGCCTT | 23 bp deleted → frameshift |
| 126 | CATCTCCGCGG-----------------------CTACCCGGCGCCTGCCTTGTCGCCTT | 23 bp deleted → frameshift |
| 120 | CATCTCCGCGGTCGCCGTCG------------------------CCTGCCTTGTCGCCTT | 24 bp deleted → no frameshift |
| 115 | CATCTCCGCGGTCGCCGTCG------------------------------------CCTT | 36 bp deleted → no frameshift |
| 115 | CATCTCCG---------------------------------GCGCCTGCCTTGTCGCCTT | 33 bp deleted → no frameshift |
| 109 | CATCTCCGCGGTCGCC----------------------------------TTGTCGCCTT | 34 bp deleted → frameshift |
| 109 | CATCTCCGCG----------------------------------CCTGCCTTGTCGCCTT | 34 bp deleted → frameshift |
| 109 | CATCTCCGC--------------------------TACCCGGCGCCTGCCTTGTCGCCTT | 26 bp deleted → frameshift |
| 105 | CATCTCCGCGGTCGCCGTCGACACGGCGCT---------------------TGTCGCCTT | 21 bp deleted → no frameshift |
| 103 | CATCTCCGCGGTCGCCG---------------------------CCTGCCTTGTCGCCTT | 27 bp deleted → no frameshift |
| 103 | CATCTCCGCGGTCG---------------------------GCGCCTGCCTTGTCGCCTT | 27 bp deleted → no frameshift |
| 99 | CATCTCCGC------------------------------------CTGCCTTGTCGCCTT | 36 bp deleted → no frameshift |
| 98 | CATCTCCGCGGTCGC----------------------------GCCTGCCTTGTCGCCTT | 28 bp deleted → frameshift |
| 89 | CATCTCCGCGGTCGCCGTCGACACGGC------------------------------CTT | 30 bp deleted → no frameshift |
| 84 | CATCTCCGCGGTCGCCGTCGACACG-------------------------------CCTT | 31 bp deleted → frameshift |
| 80 | CATCTCC--------------------------------CGGCGCCTGCCTTGTCGCCTT | 32 bp deleted → frameshift |
| 70 | CATCTCCGCGGTCGCCGTCGACACGGCGCT-----------------------------T | 29 bp deleted → frameshift |
| 63 | CATCT-------------------------------ACCCGGCGCCTGCCTTGTCGCCTT | 31 bp deleted → frameshift |
| 56 | CATCTCCGCGGTCGCCG---------------------------------------CCTT | 39 bp deleted → no frameshift |
| 56 | CATCTCC---------------------------------------TGCCTTGTCGCCTT | 39 bp deleted → no frameshift |
| 54 | CATCTCCGC------------------------------------------------CTT | 48 bp deleted → no frameshift |
| 54 | CATCTCCGC----------------------------------------CTTGTCGCCTT | 40 bp deleted → frameshift |
| 46 | CATCTCC-------------------------------------------TTGTCGCCTT | 43 bp deleted → frameshift |
| 40 | CATCTCCGCG----------------------------------------------CCTT | 46 bp deleted → frameshift |
| 36 | CATCT------------------------------------------GCCTTGTCGCCTT | 42 bp deleted → no frameshift |
| 31 | CATCTCC---------------------------------------------------TT | 51 bp deleted → no frameshift |
| 30 | CATCT----------------------------------------------TGTCGCCTT | 46 bp deleted → frameshift |
| 25 | CATCTC-------------------------------------------------GCCTT | 49 bp deleted → frameshift |
| 23 | CATC---------------------------------------------------GCCTT | 51 bp deleted → no frameshift |
| 20 | CATCT------------------------------------------------------T | 54 bp deleted → no frameshift |