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Contents:

PCR primers for off-targets of CGGACTCTCCAGAGGGGGGG AGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313818_F Primer3: not found at this Tm N.d. 1.00
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313818_R Primer3: not found at this Tm N.d. 1.00
mm3_intergenic_Gm16063|Gm2301_chr5_119626555_F TCGTCGGCAGCGTCCCACCTCCCTAGCCCAAAAT 59.3 0.75
mm3_intergenic_Gm16063|Gm2301_chr5_119626555_R GTCTCGTGGGCTCGGTCTGCAGAAGGCTATCAGCG 59.8 0.75
mm4_exon_Gm16287_chr4_139175852_F TCGTCGGCAGCGTCCTGGGGCTTCGTTCTGTGAT 60.0 0.68
mm4_exon_Gm16287_chr4_139175852_R GTCTCGTGGGCTCGGCAAGGCAATGTTGTATGGGGT 58.8 0.68
mm4_intergenic_Pgap1|Ankrd44_chr1_54615487_F Primer3: not found at this Tm N.d. 0.67
mm4_intergenic_Pgap1|Ankrd44_chr1_54615487_R Primer3: not found at this Tm N.d. 0.67
mm3_intron_Plag1_chr4_3936878_F TCGTCGGCAGCGTCCCAGAAGGTTCGCCAGTCTT 59.9 0.62
mm3_intron_Plag1_chr4_3936878_R GTCTCGTGGGCTCGGCGGGTTTTGTTAGTGGCTCC 59.1 0.62
mm3_intron_Ccdc30_chr4_119385353_F TCGTCGGCAGCGTCTGCAAATCGCCAATAGTCGC 59.6 0.58
mm3_intron_Ccdc30_chr4_119385353_R GTCTCGTGGGCTCGGAGTCTTTCCCTCCCTCACCG 60.9 0.58
mm4_intergenic_Gm24696|Myc_chr15_61984262_F TCGTCGGCAGCGTCTTCTAGGGTCTCTGCAGGCT 59.9 0.57
mm4_intergenic_Gm24696|Myc_chr15_61984262_R GTCTCGTGGGCTCGGTGGGGTGGGGAAAATCAAGG 59.8 0.57
mm4_intergenic_4930526D03Rik|Oprl1_chr2_181708878_F TCGTCGGCAGCGTCATTTTCTCTCCCGGCAGCTC 60.1 0.56
mm4_intergenic_4930526D03Rik|Oprl1_chr2_181708878_R GTCTCGTGGGCTCGGCTAAGACCCAACCAGCAGCA 59.9 0.56
mm4_intergenic_Gm26580/Kcnq5|Rims1_chr1_22043337_F TCGTCGGCAGCGTCGCAAGAACACTGGGGAGGAA 59.8 0.56
mm4_intergenic_Gm26580/Kcnq5|Rims1_chr1_22043337_R GTCTCGTGGGCTCGGTGTCCATCTCCCTCTGGCAT 60.3 0.56
mm4_intergenic_Thumpd2|Slc8a1_chr17_81178734_F TCGTCGGCAGCGTCGGAGGGCCCAAATGCTTAGT 60.0 0.56
mm4_intergenic_Thumpd2|Slc8a1_chr17_81178734_R GTCTCGTGGGCTCGGCCCACACATGCCCAACACTA 60.2 0.56
mm3_intergenic_Ercc4|Mkl2_chr16_13208211_F Primer3: not found at this Tm N.d. 0.56
mm3_intergenic_Ercc4|Mkl2_chr16_13208211_R Primer3: not found at this Tm N.d. 0.56
mm4_intron_Map4k3_chr17_80646217_F TCGTCGGCAGCGTCCACAAGCTGGGACCCCATAC 60.3 0.56
mm4_intron_Map4k3_chr17_80646217_R GTCTCGTGGGCTCGGATAAAACAGACCGCCGCCAT 60.3 0.56
mm4_intron_Llgl2_chr11_115855262_F TCGTCGGCAGCGTCGGCTCAGCAATGGAGAAGGT 60.0 0.54
mm4_intron_Llgl2_chr11_115855262_R GTCTCGTGGGCTCGGGGAGGGGTGGCAGTTCTTTT 60.1 0.54
mm4_intron_Mctp2_chr7_72203189_F TCGTCGGCAGCGTCGCCAATGGTGTAGAGCTGCT 60.3 0.53
mm4_intron_Mctp2_chr7_72203189_R GTCTCGTGGGCTCGGAGAACAAACAAATGCCTGGGC 59.9 0.53
mm4_intergenic_RP23-417D20.1|RP24-129O14.1_chr9_80971705_F TCGTCGGCAGCGTCATGGGGTGTCCTGGAGAGG 60.3 0.53
mm4_intergenic_RP23-417D20.1|RP24-129O14.1_chr9_80971705_R GTCTCGTGGGCTCGGATTTCCATGACCCCGATGCC 60.4 0.53
mm4_exon_Pnkd_chr1_74286136_F TCGTCGGCAGCGTCTCTGAAATGCGGGTGGAGAG 59.7 0.52
mm4_exon_Pnkd_chr1_74286136_R GTCTCGTGGGCTCGGTGCTCCACAGAAAAGCCACT 59.8 0.52
mm4_intergenic_Atp4b|Grk1_chr8_13403279_F TCGTCGGCAGCGTCACAACTGTATGTGCTCTGCCA 59.9 0.52
mm4_intergenic_Atp4b|Grk1_chr8_13403279_R GTCTCGTGGGCTCGGAGGGGCTGAGGAGAATTTGC 60.0 0.52
mm4_exon_B4galt2/Ccdc24_chr4_117872051_F TCGTCGGCAGCGTCCGGAGGCCTTGGAGTAACTG 60.1 0.50
mm4_exon_B4galt2/Ccdc24_chr4_117872051_R GTCTCGTGGGCTCGGCTCTGACCCTCCCTCCCTAC 60.1 0.50
mm4_intergenic_Clstn3|C1rl_chr6_124478789_F Primer3: not found at this Tm N.d. 0.46
mm4_intergenic_Clstn3|C1rl_chr6_124478789_R Primer3: not found at this Tm N.d. 0.46
mm4_intron_Camk1d_chr2_5561641_F Primer3: not found at this Tm N.d. 0.46
mm4_intron_Camk1d_chr2_5561641_R Primer3: not found at this Tm N.d. 0.46
mm4_intergenic_Gsx2|Pdgfra_chr5_75088897_F Primer3: not found at this Tm N.d. 0.45
mm4_intergenic_Gsx2|Pdgfra_chr5_75088897_R Primer3: not found at this Tm N.d. 0.45
mm4_intron_Rbfox3_chr11_118732586_F TCGTCGGCAGCGTCATGAAGGAAGGCCACACAGG 59.9 0.45
mm4_intron_Rbfox3_chr11_118732586_R GTCTCGTGGGCTCGGTGCAGGAGCTCAGAAACCAG 59.9 0.45
mm4_intergenic_Gm25781|Ipo5_chr14_120848705_F TCGTCGGCAGCGTCAAACACTGCCACCCTTTAAAAG 57.5 0.43
mm4_intergenic_Gm25781|Ipo5_chr14_120848705_R GTCTCGTGGGCTCGGTGACTCGCTGCTCTTTCTGC 60.6 0.43
mm4_intergenic_Frem2|Gm24851_chr3_53691394_F TCGTCGGCAGCGTCGCTATCTGAAGCAGCCACTGA 60.1 0.42
mm4_intergenic_Frem2|Gm24851_chr3_53691394_R GTCTCGTGGGCTCGGCAGCAAAGGCCACAAACTCC 59.9 0.42
mm4_exon_BC068157_chr8_4213642_F Primer3: not found at this Tm N.d. 0.41
mm4_exon_BC068157_chr8_4213642_R Primer3: not found at this Tm N.d. 0.41
mm4_intergenic_Gm15387|Bai1_chr15_74483491_F TCGTCGGCAGCGTCAAACCCAGGAAGCATGCAGA 59.8 0.41
mm4_intergenic_Gm15387|Bai1_chr15_74483491_R GTCTCGTGGGCTCGGAAGTGGTCGCCATCACTGTC 60.3 0.41
mm4_intergenic_Gm21614|Gm23249_chr12_91999069_F Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Gm21614|Gm23249_chr12_91999069_R Primer3: not found at this Tm N.d. 0.40
mm4_intron_Susd4_chr1_182834708_F Primer3: not found at this Tm N.d. 0.39
mm4_intron_Susd4_chr1_182834708_R Primer3: not found at this Tm N.d. 0.39
mm4_exon_Prkcd_chr14_30595733_F TCGTCGGCAGCGTCTGATGGCAAAGGGAAGGCAA 60.1 0.39
mm4_exon_Prkcd_chr14_30595733_R GTCTCGTGGGCTCGGGACTGGCTGATTTCTGCTGC 59.5 0.39
mm4_intron_Map1b_chr13_99465616_F TCGTCGGCAGCGTCTCTTTTCCAGCATGTAAACGCA 59.1 0.38
mm4_intron_Map1b_chr13_99465616_R GTCTCGTGGGCTCGGGTGGACGGCCCTTTTCTCTT 60.2 0.38
mm4_intergenic_Gm12700|Cachd1_chr4_100642133_F Primer3: not found at this Tm N.d. 0.37
mm4_intergenic_Gm12700|Cachd1_chr4_100642133_R Primer3: not found at this Tm N.d. 0.37
mm4_intergenic_Gm25684|Gm9966_chr7_95867953_F Primer3: not found at this Tm N.d. 0.37
mm4_intergenic_Gm25684|Gm9966_chr7_95867953_R Primer3: not found at this Tm N.d. 0.37
mm4_intron_Prkca_chr11_108032698_F TCGTCGGCAGCGTCACGAGGCCATCAACAGTGAG 60.0 0.36
mm4_intron_Prkca_chr11_108032698_R GTCTCGTGGGCTCGGCCTGGATGGTTACTCGGCTC 59.8 0.36
mm4_intergenic_Tcf7|A630014C17Rik_chr11_52283453_F TCGTCGGCAGCGTCGTCGGAACATCAAAGGCACC 59.4 0.36
mm4_intergenic_Tcf7|A630014C17Rik_chr11_52283453_R GTCTCGTGGGCTCGGTTATTCCCAGTGGGCCTGTC 59.3 0.36
mm4_intergenic_Atp1a3|Grik5_chr7_25008251_F TCGTCGGCAGCGTCGTGCATAACGCTCCCAATGC 60.2 0.35
mm4_intergenic_Atp1a3|Grik5_chr7_25008251_R GTCTCGTGGGCTCGGTCCTCCATCCCTGTGACCTT 59.8 0.35
mm4_intergenic_Gm2348|Slc1a3_chr15_8571523_F Primer3: not found at this Tm N.d. 0.35
mm4_intergenic_Gm2348|Slc1a3_chr15_8571523_R Primer3: not found at this Tm N.d. 0.35
mm4_intergenic_Sorbs2|Gm25309_chr8_45841719_F Primer3: not found at this Tm N.d. 0.34
mm4_intergenic_Sorbs2|Gm25309_chr8_45841719_R Primer3: not found at this Tm N.d. 0.34
mm4_intergenic_PVT1_3|Ndufa4_chr6_11485692_F TCGTCGGCAGCGTCATGCACGGGACCAGAACATT 59.9 0.34
mm4_intergenic_PVT1_3|Ndufa4_chr6_11485692_R GTCTCGTGGGCTCGGAACCTGGAAATGAGGTGCCA 59.5 0.34
mm4_intergenic_Gm10293|Gm22798_chr3_52615206_F Primer3: not found at this Tm N.d. 0.33
mm4_intergenic_Gm10293|Gm22798_chr3_52615206_R Primer3: not found at this Tm N.d. 0.33
mm4_intron_Csmd2_chr4_128299765_F TCGTCGGCAGCGTCAGTGCAATTTCTACACCAATTGT 57.2 0.33
mm4_intron_Csmd2_chr4_128299765_R GTCTCGTGGGCTCGGTAGTAACAGTGAGGGCCACC 58.7 0.33
mm4_intergenic_Gpr158|Gm13337_chr2_21961447_F TCGTCGGCAGCGTCGAGTCAGCCCAGAAACCACA 59.8 0.31
mm4_intergenic_Gpr158|Gm13337_chr2_21961447_R GTCTCGTGGGCTCGGTGCAGAAGGGATTGCCTGAA 59.5 0.31
mm4_exon_Anks6_chr4_47049392_F Primer3: not found at this Tm N.d. 0.31
mm4_exon_Anks6_chr4_47049392_R Primer3: not found at this Tm N.d. 0.31
mm4_intergenic_Rhobtb1|Cdk1_chr10_69300423_F TCGTCGGCAGCGTCACAGAAGAGAGTGGGGAAAGC 59.6 0.29
mm4_intergenic_Rhobtb1|Cdk1_chr10_69300423_R GTCTCGTGGGCTCGGTGCTATCCCCAGGGTTCAGA 59.9 0.29
mm4_intron_Ano10_chr9_122265209_F Primer3: not found at this Tm N.d. 0.29
mm4_intron_Ano10_chr9_122265209_R Primer3: not found at this Tm N.d. 0.29
mm4_intergenic_Kcnk5|Gm24912_chr14_20193135_F Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_Kcnk5|Gm24912_chr14_20193135_R Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_Lce3f|Crct1_chr3_92999996_F TCGTCGGCAGCGTCGGACAGAAATTTGGGAGCTGG 59.1 0.27
mm4_intergenic_Lce3f|Crct1_chr3_92999996_R GTCTCGTGGGCTCGGCCTGAGACAACCTGGAAGCA 59.6 0.27
mm4_intergenic_Gm23867|Gm15204_chrX_162333517_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm23867|Gm15204_chrX_162333517_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm5899|Gm23884_chr7_94327357_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm5899|Gm23884_chr7_94327357_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Camta1|4930589P08Rik_chr4_151349014_F TCGTCGGCAGCGTCCGAAAGGACAGCCACTTCCT 59.9 0.27
mm4_intergenic_Camta1|4930589P08Rik_chr4_151349014_R GTCTCGTGGGCTCGGGAGACATCCTATGCCTCGACA 59.0 0.27
mm4_intron_Epb4.1l1_chr2_156509371_F TCGTCGGCAGCGTCGATGGAGGTATGGCTCAGGC 59.9 0.27
mm4_intron_Epb4.1l1_chr2_156509371_R GTCTCGTGGGCTCGGTACCGGCTGTCTCTTCTGGA 59.9 0.27
mm4_intergenic_Gm22302|Uri1_chr7_37829835_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm22302|Uri1_chr7_37829835_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm7275|Gm7204_chr16_48160037_F TCGTCGGCAGCGTCACAACAACAGCAAGCCATGT 58.8 0.27
mm4_intergenic_Gm7275|Gm7204_chr16_48160037_R GTCTCGTGGGCTCGGAATATGCCCAGCTGGACCAC 60.1 0.27
mm4_intergenic_Gm15270/Ctgf|Enpp1_chr10_24631982_F TCGTCGGCAGCGTCACAAGGAAAAGGTGGGCACA 60.0 0.27
mm4_intergenic_Gm15270/Ctgf|Enpp1_chr10_24631982_R GTCTCGTGGGCTCGGTGGAGCCTCGTCTGTATTTCG 59.8 0.27
mm4_intergenic_Gm24291|Mon1b_chr8_113551664_F TCGTCGGCAGCGTCGCGTTGTTCTGAACCGGAAG 59.7 0.26
mm4_intergenic_Gm24291|Mon1b_chr8_113551664_R GTCTCGTGGGCTCGGCCTCTCCTTCCTCCCTTGGA 59.9 0.26
mm4_intron_Aprt_chr8_122575906_F TCGTCGGCAGCGTCCACTTACCAGGGCCACTCTG 60.0 0.26
mm4_intron_Aprt_chr8_122575906_R GTCTCGTGGGCTCGGACAACTGCTGTCTACCTGGC 59.9 0.26
mm4_intergenic_Nrsn1|Tpi-rs8_chr13_25401713_F Primer3: not found at this Tm N.d. 0.26
mm4_intergenic_Nrsn1|Tpi-rs8_chr13_25401713_R Primer3: not found at this Tm N.d. 0.26
mm4_intron_Slc22a5_chr11_53887775_F TCGTCGGCAGCGTCTGCCATGAAACTGCAAAGGC 59.9 0.25
mm4_intron_Slc22a5_chr11_53887775_R GTCTCGTGGGCTCGGCCTGCCTGTGCTAGCCAT 59.4 0.25
mm4_exon_D630003M21Rik_chr2_158204719_F TCGTCGGCAGCGTCGCAAGCTGGCCAGAGTAGTT 60.3 0.25
mm4_exon_D630003M21Rik_chr2_158204719_R GTCTCGTGGGCTCGGTGAGCAAGTCCAAGGAGCTG 59.9 0.25
mm4_intergenic_Tulp1|Fkbp5_chr17_28394226_F Primer3: not found at this Tm N.d. 0.25
mm4_intergenic_Tulp1|Fkbp5_chr17_28394226_R Primer3: not found at this Tm N.d. 0.25
mm4_intergenic_Rgag1|Gm4760_chrX_143181211_F Primer3: not found at this Tm N.d. 0.23
mm4_intergenic_Rgag1|Gm4760_chrX_143181211_R Primer3: not found at this Tm N.d. 0.23
mm4_intergenic_Gm17384|3110047P20Rik/Gm9954_chr5_63124570_F Primer3: not found at this Tm N.d. 0.23
mm4_intergenic_Gm17384|3110047P20Rik/Gm9954_chr5_63124570_R Primer3: not found at this Tm N.d. 0.23
mm4_intergenic_Gm22597|Gm24514_chr18_55410022_F TCGTCGGCAGCGTCTGTGGCAGATGTGAAGGTCG 60.3 0.22
mm4_intergenic_Gm22597|Gm24514_chr18_55410022_R GTCTCGTGGGCTCGGTTTTGCTGTCTCACCGTTGC 59.6 0.22
mm4_intron_Snd1_chr6_28900998_F TCGTCGGCAGCGTCAAAGGCCAAGGAGATGAGGC 60.0 0.22
mm4_intron_Snd1_chr6_28900998_R GTCTCGTGGGCTCGGAAGTCCTGGTGTGTGTGTGA 59.0 0.22
mm4_intron_Tjp2_chr19_24221091_F TCGTCGGCAGCGTCTCCACTCAGCCTTGCAGAAG 59.9 0.22
mm4_intron_Tjp2_chr19_24221091_R GTCTCGTGGGCTCGGACTCAGCCAAGATCTAGCAAGA 58.8 0.22
mm4_intergenic_Gjb2|Gm4491_chr14_57106694_F Primer3: not found at this Tm N.d. 0.22
mm4_intergenic_Gjb2|Gm4491_chr14_57106694_R Primer3: not found at this Tm N.d. 0.22
mm4_intergenic_Nps|4930544L04Rik_chr7_135388236_F TCGTCGGCAGCGTCTATGTGCAGCAATGGGCCTG 61.0 0.22
mm4_intergenic_Nps|4930544L04Rik_chr7_135388236_R GTCTCGTGGGCTCGGTTGCAGCCATCCAGTGTTGA 60.1 0.22
mm4_intron_Npy2r_chr3_82542035_F Primer3: not found at this Tm N.d. 0.22
mm4_intron_Npy2r_chr3_82542035_R Primer3: not found at this Tm N.d. 0.22
mm4_exon_Igf2bp1/Mir3063_chr11_95961323_F TCGTCGGCAGCGTCGGGAAGAGCCGGCTAAGGTA 61.0 0.22
mm4_exon_Igf2bp1/Mir3063_chr11_95961323_R GTCTCGTGGGCTCGGAGCAGAGCTTTAGCCCTTGG 60.0 0.22
mm4_intergenic_Olfr519|Nlrp10_chr7_108899322_F Primer3: not found at this Tm N.d. 0.22
mm4_intergenic_Olfr519|Nlrp10_chr7_108899322_R Primer3: not found at this Tm N.d. 0.22
mm4_intron_Shfm1_chr6_6572276_F Primer3: not found at this Tm N.d. 0.22
mm4_intron_Shfm1_chr6_6572276_R Primer3: not found at this Tm N.d. 0.22
mm4_exon_Nodal_chr10_61418205_F Primer3: not found at this Tm N.d. 0.21
mm4_exon_Nodal_chr10_61418205_R Primer3: not found at this Tm N.d. 0.21
mm3_intergenic_RP24-234J3.4/Gm21971|Gm21971_chr12_102726023_F Primer3: not found at this Tm N.d. 0.21
mm3_intergenic_RP24-234J3.4/Gm21971|Gm21971_chr12_102726023_R Primer3: not found at this Tm N.d. 0.21
mm4_intron_Pqlc3_chr12_16991776_F TCGTCGGCAGCGTCCCTGTCCCTGTGTCCTGAAC 59.9 0.21
mm4_intron_Pqlc3_chr12_16991776_R GTCTCGTGGGCTCGGGGTGTACTTGGAGGGAGCTG 59.7 0.21
mm4_exon_Tbata_chr10_61188831_F TCGTCGGCAGCGTCTCTACCTTGACTCCAGCCCC 60.6 0.21
mm4_exon_Tbata_chr10_61188831_R GTCTCGTGGGCTCGGGGAGGGGACACTTCTCTTCT 58.0 0.21
mm4_intergenic_Gm7168|Dact2_chr17_14105711_F TCGTCGGCAGCGTCCAACAGGTCTTCCAGCTCGT 59.9 0.20
mm4_intergenic_Gm7168|Dact2_chr17_14105711_R GTCTCGTGGGCTCGGGTCACCATACCTGAGCTGCA 59.7 0.20
mm4_intergenic_Tm4sf20|Gm22396/Agfg1_chr1_82833076_F TCGTCGGCAGCGTCTGGCTTCCCTACACTCTGGA 59.8 0.20
mm4_intergenic_Tm4sf20|Gm22396/Agfg1_chr1_82833076_R GTCTCGTGGGCTCGGTGATGGCGTGTTCCCATCTC 60.1 0.20
mm4_exon_Shbg_chr11_69615560_F TCGTCGGCAGCGTCTACCTGGTAAAGCCCCAAGG 59.0 0.20
mm4_exon_Shbg_chr11_69615560_R GTCTCGTGGGCTCGGGGTCTTGAGCCAAGGACCAA 59.8 0.20
mm4_intron_Grm2_chr9_106649249_F TCGTCGGCAGCGTCACACAGGTTCCACAGCAACA 60.0 0.20
mm4_intron_Grm2_chr9_106649249_R GTCTCGTGGGCTCGGCAATATATTGCCTCCCTCCCCA 59.3 0.20
mm2_intergenic_E030044B06Rik|mmu-mir-8092/Zfp518a_chr19_40874234_F Primer3: not found at this Tm N.d. 0.19
mm2_intergenic_E030044B06Rik|mmu-mir-8092/Zfp518a_chr19_40874234_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm27043|Gm25020_chr13_17129549_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm27043|Gm25020_chr13_17129549_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Prdx6|Tnfsf4_chr1_161288471_F TCGTCGGCAGCGTCTCTCTGGCTGTGCTCTCTCT 59.9 0.19
mm4_intergenic_Prdx6|Tnfsf4_chr1_161288471_R GTCTCGTGGGCTCGGATTGGGAGTGAGCCTGTGAC 59.6 0.19
mm4_intergenic_Gm17118|Cacna2d2_chr9_107460849_F TCGTCGGCAGCGTCCCAGCTAAGTCTGCCAGTCC 60.1 0.17
mm4_intergenic_Gm17118|Cacna2d2_chr9_107460849_R GTCTCGTGGGCTCGGGAGCTTGGGGCAGACAAGAT 60.0 0.17
mm4_exon_Dpy19l4/Gm26663_chr4_11322139_F TCGTCGGCAGCGTCCTCCGAGTATCCAAACCCCG 59.8 0.17
mm4_exon_Dpy19l4/Gm26663_chr4_11322139_R GTCTCGTGGGCTCGGCTCCCTGCACTCTGGTTCC 59.7 0.17
mm4_intron_Lasp1_chr11_97808235_F TCGTCGGCAGCGTCGGGCTCACACAAACCCTGAT 60.2 0.17
mm4_intron_Lasp1_chr11_97808235_R GTCTCGTGGGCTCGGCTAAAGCAGTCCAGCACCCA 59.9 0.17
mm4_exon_C230012O17Rik_chr4_24964048_F TCGTCGGCAGCGTCCACATGATGCTTTGTGATCCCC 59.9 0.17
mm4_exon_C230012O17Rik_chr4_24964048_R GTCTCGTGGGCTCGGTCAGTTGTGTGCCTGCTTCT 59.8 0.17
mm4_intron_Zc3h12c_chr9_52167947_F Primer3: not found at this Tm N.d. 0.17
mm4_intron_Zc3h12c_chr9_52167947_R Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Agfg1|Slc19a3_chr1_82911557_F Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Agfg1|Slc19a3_chr1_82911557_R Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Frs2|Gm26311_chr10_117167749_F TCGTCGGCAGCGTCACAAAAGGCTGTAGGGGACA 58.8 0.16
mm4_intergenic_Frs2|Gm26311_chr10_117167749_R GTCTCGTGGGCTCGGTCCCTTACACACACCTCGAA 58.2 0.16
mm3_intergenic_Gm13232|Gm13244_chr4_145745017_F Primer3: not found at this Tm N.d. 0.16
mm3_intergenic_Gm13232|Gm13244_chr4_145745017_R Primer3: not found at this Tm N.d. 0.16
mm4_intron_Park2_chr17_10963721_F TCGTCGGCAGCGTCCTCCAAGTCTTTGTGAGGCCT 59.9 0.15
mm4_intron_Park2_chr17_10963721_R GTCTCGTGGGCTCGGACCATTATGCAATGAAAGCCATGT 59.8 0.15
mm4_intergenic_Gm833|Gm25779_chr4_152890532_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm833|Gm25779_chr4_152890532_R Primer3: not found at this Tm N.d. 0.15
mm3_intergenic_Gm23558|Galc_chr12_97676887_F TCGTCGGCAGCGTCAGCAGTTTGGGGAAGCTAGA 58.6 0.15
mm3_intergenic_Gm23558|Galc_chr12_97676887_R GTCTCGTGGGCTCGGGAGAGGCTAGAGGGATCCCA 59.5 0.15
mm4_intergenic_Gm22618|Gm22976_chr5_50435160_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Gm22618|Gm22976_chr5_50435160_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Ppargc1a|mmu-mir-6417_chr5_52115284_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Ppargc1a|mmu-mir-6417_chr5_52115284_R Primer3: not found at this Tm N.d. 0.14
mm4_intron_Lmtk3_chr7_45791498_F Primer3: not found at this Tm N.d. 0.14
mm4_intron_Lmtk3_chr7_45791498_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Crybb1|Tpst2_chr5_112269688_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Crybb1|Tpst2_chr5_112269688_R Primer3: not found at this Tm N.d. 0.14
mm4_intron_Reln_chr5_21900575_F TCGTCGGCAGCGTCCTCTGGCAGGCTGTCTTACC 60.1 0.13
mm4_intron_Reln_chr5_21900575_R GTCTCGTGGGCTCGGGGGAAAGCGAGCTCCATCAT 60.1 0.13
mm4_intergenic_Gm23980|Gm22810_chr15_24756775_F TCGTCGGCAGCGTCGCAAGGTAGCCATGAAGAACC 59.2 0.13
mm4_intergenic_Gm23980|Gm22810_chr15_24756775_R GTCTCGTGGGCTCGGCTGAATGCAGGAGCTGAGAGT 59.7 0.13
mm3_intergenic_Hormad2|Mtmr3_chr11_4459526_F Primer3: not found at this Tm N.d. 0.13
mm3_intergenic_Hormad2|Mtmr3_chr11_4459526_R Primer3: not found at this Tm N.d. 0.13
mm4_intron_Dock2_chr11_34287476_F Primer3: not found at this Tm N.d. 0.13
mm4_intron_Dock2_chr11_34287476_R Primer3: not found at this Tm N.d. 0.13
mm4_intron_Ttll6_chr11_96146342_F TCGTCGGCAGCGTCAAGCTTTCCCAGAAGCTGCT 59.8 0.13
mm4_intron_Ttll6_chr11_96146342_R GTCTCGTGGGCTCGGGGCAGCTAGAAGGCCAGTTC 60.7 0.13
mm4_intron_Atp2b4_chr1_133767081_F Primer3: not found at this Tm N.d. 0.13
mm4_intron_Atp2b4_chr1_133767081_R Primer3: not found at this Tm N.d. 0.13
mm4_exon_Nono_chrX_101429638_F TCGTCGGCAGCGTCACTGGCAGTTCACATAGCCA 59.3 0.13
mm4_exon_Nono_chrX_101429638_R GTCTCGTGGGCTCGGCCACCGTCCCGAGAAAAGAG 60.3 0.13
mm4_exon_Col9a1_chr1_24179521_F TCGTCGGCAGCGTCTTGAGAAGGCTGCATCTCGA 59.1 0.13
mm4_exon_Col9a1_chr1_24179521_R GTCTCGTGGGCTCGGCCACTTGTAAGGCTGTGGAT 57.5 0.13
mm4_intergenic_Gm20696/Foxp1|Gm20705_chr6_99382635_F Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Gm20696/Foxp1|Gm20705_chr6_99382635_R Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Gm27043|Gm25020_chr13_17071225_F TCGTCGGCAGCGTCTCATGTTCATGAGTAAACTGAGACT 58.0 0.12
mm4_intergenic_Gm27043|Gm25020_chr13_17071225_R GTCTCGTGGGCTCGGTCCAATAAAACCCCTCTTCCCA 59.0 0.12
mm4_intergenic_Fbxl17|4930405O22Rik_chr17_63245524_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Fbxl17|4930405O22Rik_chr17_63245524_R Primer3: not found at this Tm N.d. 0.12
mm4_exon_Slmap_chr14_26534421_F TCGTCGGCAGCGTCTTGGTTTCTCGGAGCTCTCG 59.7 0.12
mm4_exon_Slmap_chr14_26534421_R GTCTCGTGGGCTCGGCAGTCCCTGCGATGCGAC 60.8 0.12
mm4_intergenic_Ptplb|Adcy5_chr16_35117462_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Ptplb|Adcy5_chr16_35117462_R Primer3: not found at this Tm N.d. 0.12
mm4_intron_Nfasc_chr1_132635793_F TCGTCGGCAGCGTCCAGGGCCAGAGCAAACCTC 60.6 0.12
mm4_intron_Nfasc_chr1_132635793_R GTCTCGTGGGCTCGGACCAAGGCCAGCTCTCTTTC 59.9 0.12
mm4_intergenic_Pard3|Gm6921_chr8_127518634_F TCGTCGGCAGCGTCTAGCTGCATTTTCCAAGCCG 59.1 0.12
mm4_intergenic_Pard3|Gm6921_chr8_127518634_R GTCTCGTGGGCTCGGCAGTGTTCACTTTGGAAGGCA 58.9 0.12
mm4_intergenic_Rarb|Thrb_chr14_16816793_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Rarb|Thrb_chr14_16816793_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm24503|Ccrn4l_chr3_50900570_F TCGTCGGCAGCGTCCCCAAACTACCAGGTCAGGG 59.6 0.12
mm4_intergenic_Gm24503|Ccrn4l_chr3_50900570_R GTCTCGTGGGCTCGGACCCAGTCATAGGAGGCTCA 59.6 0.12
mm4_intergenic_Eif3b|Chst12_chr5_140485967_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Eif3b|Chst12_chr5_140485967_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm22000|Myl4/Gm22000_chr11_104559908_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm22000|Myl4/Gm22000_chr11_104559908_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Cdkn2b|Gm12609_chr4_89312388_F TCGTCGGCAGCGTCTGCTTCTGGGTGAAACGTCT 59.5 0.10
mm4_intergenic_Cdkn2b|Gm12609_chr4_89312388_R GTCTCGTGGGCTCGGTTCCTCCGCCTCCCATTCTA 60.0 0.10
mm4_intergenic_Gm24444|Mgmt_chr7_136607308_F TCGTCGGCAGCGTCTGCATGCTTGTGAGGTCATCA 60.2 0.10
mm4_intergenic_Gm24444|Mgmt_chr7_136607308_R GTCTCGTGGGCTCGGTCAGAATTCCATGCATTTTAGCCA 59.2 0.10
mm4_intron_Gli2_chr1_118887044_F TCGTCGGCAGCGTCAAAGATGAGGCTTGCCCGTT 60.2 0.10
mm4_intron_Gli2_chr1_118887044_R GTCTCGTGGGCTCGGGTGCTCACAGGACTCCTTCC 60.0 0.10
mm4_intergenic_Trhr2|Gm20681_chr8_122363269_F TCGTCGGCAGCGTCGTCCCAGGGCCAATCTGTAC 60.1 0.10
mm4_intergenic_Trhr2|Gm20681_chr8_122363269_R GTCTCGTGGGCTCGGCAAGCCCAAGAGGACAGTGT 59.8 0.10
mm4_intron_Nxph1_chr6_9036939_F Primer3: not found at this Tm N.d. 0.09
mm4_intron_Nxph1_chr6_9036939_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Cep97|RPL24/RP24-288C12.6_chr16_55940243_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Cep97|RPL24/RP24-288C12.6_chr16_55940243_R Primer3: not found at this Tm N.d. 0.09
mm4_intron_Afap1l2_chr19_56930610_F Primer3: not found at this Tm N.d. 0.08
mm4_intron_Afap1l2_chr19_56930610_R Primer3: not found at this Tm N.d. 0.08
mm4_intron_Tpd52_chr3_8993108_F Primer3: not found at this Tm N.d. 0.08
mm4_intron_Tpd52_chr3_8993108_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Cd274|Pdcd1lg2_chr19_29408114_F TCGTCGGCAGCGTCCTCTGACAACCCCTTCACCC 59.9 0.08
mm4_intergenic_Cd274|Pdcd1lg2_chr19_29408114_R GTCTCGTGGGCTCGGGCTCTGACTCCTGGCCAAAA 60.2 0.08
mm4_exon_Pam_chr1_97977131_F Primer3: not found at this Tm N.d. 0.08
mm4_exon_Pam_chr1_97977131_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm21786|Mpp6_chr6_50045954_F TCGTCGGCAGCGTCTGGCCTCTTTCTGAAGTGCT 59.2 0.08
mm4_intergenic_Gm21786|Mpp6_chr6_50045954_R GTCTCGTGGGCTCGGTGCTCAAGAACAGCGAGAGG 60.0 0.08
mm4_intron_Mllt3_chr4_87907891_F Primer3: not found at this Tm N.d. 0.08
mm4_intron_Mllt3_chr4_87907891_R Primer3: not found at this Tm N.d. 0.08
mm4_intron_3100003L05Rik_chr7_124659986_F Primer3: not found at this Tm N.d. 0.08
mm4_intron_3100003L05Rik_chr7_124659986_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Onecut1/Gm20649|Fam214a_chr9_74907025_F TCGTCGGCAGCGTCCTCTGAGGTCTGTGTGGCTG 60.0 0.08
mm4_intergenic_Onecut1/Gm20649|Fam214a_chr9_74907025_R GTCTCGTGGGCTCGGCCAAGTTCAGGGACCCACTT 59.5 0.08
mm4_intron_Aff1_chr5_103749979_F TCGTCGGCAGCGTCGAGGAGCCCTGCTTGGATAC 59.8 0.08
mm4_intron_Aff1_chr5_103749979_R GTCTCGTGGGCTCGGTAAGGACAGGGCCACTTCCA 60.4 0.08
mm4_intron_Ank3_chr10_69940138_F TCGTCGGCAGCGTCAAAGAGAAGTGGGGCAGGTG 59.8 0.07
mm4_intron_Ank3_chr10_69940138_R GTCTCGTGGGCTCGGCAGAGACAGGAGCAGGCATC 60.1 0.07
mm4_intergenic_Ppp2r5a|Dtl_chr1_191397144_F TCGTCGGCAGCGTCGGGGTGGAGGAAGGAGTTTG 59.9 0.07
mm4_intergenic_Ppp2r5a|Dtl_chr1_191397144_R GTCTCGTGGGCTCGGGAGGGCACTGGCATGCTTTC 61.9 0.07
mm4_intergenic_Gm13237|Gm13233_chr4_146579899_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm13237|Gm13233_chr4_146579899_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm13167|Gm13169_chr4_146239254_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm13167|Gm13169_chr4_146239254_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm24979|Cdc5l_chr17_45342185_F TCGTCGGCAGCGTCCAGTCCTGGGGCTTTGTAGG 60.0 0.07
mm4_intergenic_Gm24979|Cdc5l_chr17_45342185_R GTCTCGTGGGCTCGGACAAAGAGAAAGGCTGCTGTG 59.0 0.07
mm4_intergenic_Gm16314|Gnb1l_chr16_18503819_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm16314|Gnb1l_chr16_18503819_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm12439|Gm12438_chr4_48720104_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm12439|Gm12438_chr4_48720104_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_St3gal1|Zfat_chr15_67116252_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_St3gal1|Zfat_chr15_67116252_R Primer3: not found at this Tm N.d. 0.06
mm4_exon_Wsb2_chr5_117365251_F Primer3: not found at this Tm N.d. 0.06
mm4_exon_Wsb2_chr5_117365251_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_2810408A11Rik_chr11_69898594_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_2810408A11Rik_chr11_69898594_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Lep_chr6_29070427_F TCGTCGGCAGCGTCAAGAGTCATCAGGGCTGCAC 60.0 0.06
mm4_intron_Lep_chr6_29070427_R GTCTCGTGGGCTCGGCATTAGGGTCAAAGCCGTGC 59.5 0.06
mm4_intron_Ttyh1_chr7_4123683_F TCGTCGGCAGCGTCGCGCCCTATAGCCAAGATTT 58.1 0.05
mm4_intron_Ttyh1_chr7_4123683_R GTCTCGTGGGCTCGGTGTTCCTAGGCTCTGAGGGG 60.3 0.05
mm4_exon_Ece1_chr4_137963009_F TCGTCGGCAGCGTCAAAAGGGAAGGAGCTGCAGC 60.8 0.05
mm4_exon_Ece1_chr4_137963009_R GTCTCGTGGGCTCGGGTGGTGAGGCTCCATCCATC 60.1 0.05
mm4_exon_Sh3rf2_chr18_42101714_F TCGTCGGCAGCGTCGCCAGCTCTGTGGAAGTCAT 60.0 0.05
mm4_exon_Sh3rf2_chr18_42101714_R GTCTCGTGGGCTCGGCCTTGAGGAAGGTCAGGCAG 60.0 0.05
mm4_intergenic_Nlgn2|Tmem256_chr11_69837852_F TCGTCGGCAGCGTCCACACCCCTCGCTTGCAC 61.3 0.05
mm4_intergenic_Nlgn2|Tmem256_chr11_69837852_R GTCTCGTGGGCTCGGCCCTGCTCTAGTTCGCTACG 59.9 0.05
mm4_intergenic_Gm20424|Lbx1_chr19_45197137_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm20424|Lbx1_chr19_45197137_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm25550|Cdh9_chr15_16151663_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm25550|Cdh9_chr15_16151663_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Olfr721-ps1|Slc4a7_chr14_14591590_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Olfr721-ps1|Slc4a7_chr14_14591590_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_A330033J07Rik|Id4/A330033J07Rik_chr13_48240776_F TCGTCGGCAGCGTCCTGAGCTGTTTTGCACACCA 59.2 0.04
mm4_intergenic_A330033J07Rik|Id4/A330033J07Rik_chr13_48240776_R GTCTCGTGGGCTCGGGGAAACGTACCTGTTCGGGT 59.9 0.04
mm4_exon_Zfp324_chr7_12966240_F TCGTCGGCAGCGTCCTATGCCCAGGTCTCTCGC 59.6 0.04
mm4_exon_Zfp324_chr7_12966240_R GTCTCGTGGGCTCGGCCTGGTGGGTCCTTGGAAAG 60.2 0.04
mm4_exon_Asmt_chrX_170676323_F TCGTCGGCAGCGTCCACCGGAGACAGGAAGTGAC 60.0 0.04
mm4_exon_Asmt_chrX_170676323_R GTCTCGTGGGCTCGGGTCTCGAACACGGTGACCTC 60.3 0.04
mm4_intergenic_Zfp385c|Gm11547_chr11_100673393_F TCGTCGGCAGCGTCGAGTAGCTGCCAGGAGCCTC 62.0 0.04
mm4_intergenic_Zfp385c|Gm11547_chr11_100673393_R GTCTCGTGGGCTCGGCGACGGCCTAGCTCTTTAGG 59.9 0.04
mm4_intergenic_Cacna1e|Gm9530_chr1_155053516_F TCGTCGGCAGCGTCGGGTGAGGACAGAGAAGGGA 60.2 0.04
mm4_intergenic_Cacna1e|Gm9530_chr1_155053516_R GTCTCGTGGGCTCGGCCAGGCTGAGCTTTGAGACA 59.9 0.04
mm4_intron_Git2_chr5_114766646_F TCGTCGGCAGCGTCTTGGGGCTGGTTTGGAAACA 60.3 0.04
mm4_intron_Git2_chr5_114766646_R GTCTCGTGGGCTCGGAGTGGAGTCCAGCTTTGCC 59.9 0.04
mm4_exon_Tmem92_chr11_94777676_F TCGTCGGCAGCGTCCCTCTGCAGCACACACAGTA 59.9 0.04
mm4_exon_Tmem92_chr11_94777676_R GTCTCGTGGGCTCGGTTTTGTCCTGTGGCTGAGGG 60.1 0.04
mm4_intergenic_Manbal|Src_chr2_157396803_F TCGTCGGCAGCGTCTGCCTGTGTTGCTACTGAGG 59.9 0.04
mm4_intergenic_Manbal|Src_chr2_157396803_R GTCTCGTGGGCTCGGCAGAGCTGCCCTTTCTCTCC 60.1 0.04
mm4_intron_Tmem117_chr15_94793910_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Tmem117_chr15_94793910_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Il13|Rps2-ps4_chr11_53635711_F TCGTCGGCAGCGTCACACCCGCACATAGCCTTTT 60.2 0.03
mm4_intergenic_Il13|Rps2-ps4_chr11_53635711_R GTCTCGTGGGCTCGGGGTGAGGGTGCATCTTGACA 59.9 0.03
mm4_exon_Sgsm3_chr15_81009392_F Primer3: not found at this Tm N.d. 0.03
mm4_exon_Sgsm3_chr15_81009392_R Primer3: not found at this Tm N.d. 0.03
mm3_exon_Sympk_chr7_19045830_F TCGTCGGCAGCGTCTGAGAGAGGGAGCAGGACAA 59.8 0.03
mm3_exon_Sympk_chr7_19045830_R GTCTCGTGGGCTCGGTCTCCAAGTGTCGACATGCC 60.0 0.03
mm4_intergenic_Fut10|Gm23539_chr8_31384959_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Fut10|Gm23539_chr8_31384959_R Primer3: not found at this Tm N.d. 0.02
mm4_exon_Gm10612/Cbfa2t3_chr8_122698857_F Primer3: not found at this Tm N.d. 0.02
mm4_exon_Gm10612/Cbfa2t3_chr8_122698857_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Uncx|Micall2_chr5_139550177_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Uncx|Micall2_chr5_139550177_R Primer3: not found at this Tm N.d. 0.02
mm4_exon_Prkar1a_chr11_109651059_F Primer3: not found at this Tm N.d. 0.02
mm4_exon_Prkar1a_chr11_109651059_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_2810442N19Rik/Dnm3|Pigc_chr1_162022336_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_2810442N19Rik/Dnm3|Pigc_chr1_162022336_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Hs1bp3_chr12_8324507_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Hs1bp3_chr12_8324507_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm3667|Gm6356_chr14_6951004_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm3667|Gm6356_chr14_6951004_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm3091|Gm3269_chr14_4799158_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm3091|Gm3269_chr14_4799158_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm26630|Gm8356_chr14_6514438_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm26630|Gm8356_chr14_6514438_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Zfp64_chr2_168947343_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Zfp64_chr2_168947343_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Pitx1_chr13_55833965_F TCGTCGGCAGCGTCGGTTCACTGAAGCAGGTCGA 59.9 0.00
mm4_intron_Pitx1_chr13_55833965_R GTCTCGTGGGCTCGGCCCACAACAATGCAGGCATC 60.1 0.00
mm4_intron_Parva_chr7_112557588_F TCGTCGGCAGCGTCCCTAGCTCTCACACCCTCCT 60.0 0.00
mm4_intron_Parva_chr7_112557588_R GTCTCGTGGGCTCGGCTAGACTGCTGGTGACCGAC 59.8 0.00
mm4_intron_Ifngr2_chr16_91560331_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Ifngr2_chr16_91560331_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Eml5_chr12_98879947_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Eml5_chr12_98879947_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Clstn2_chr9_97796195_F TCGTCGGCAGCGTCCATGTGACTGAAAGGAGGCCT 59.9 0.00
mm4_intron_Clstn2_chr9_97796195_R GTCTCGTGGGCTCGGGCACTGGCTATAGGAAACATGG 59.1 0.00
mm4_intron_Ccdc11_chr18_74339668_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Ccdc11_chr18_74339668_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Arhgef17_chr7_100918682_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Arhgef17_chr7_100918682_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Aff3_chr1_38639900_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Aff3_chr1_38639900_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Six2|Srbd1_chr17_85781894_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Six2|Srbd1_chr17_85781894_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Pitpnc1|Psmd12_chr11_107470707_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Pitpnc1|Psmd12_chr11_107470707_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Klf2|Eps15l1_chr8_72333059_F TCGTCGGCAGCGTCGAAAGGACCCTGCAGCTCTT 59.9 0.00
mm4_intergenic_Klf2|Eps15l1_chr8_72333059_R GTCTCGTGGGCTCGGAGGGCTCTCACCATAGGAGT 59.3 0.00
mm4_intergenic_Gm26556|Ppp1r9a_chr6_4971080_F TCGTCGGCAGCGTCAATTCTGACTGGGTGGAGGC 59.6 0.00
mm4_intergenic_Gm26556|Ppp1r9a_chr6_4971080_R GTCTCGTGGGCTCGGTGCCATCCCTCAATCCAAGT 58.9 0.00
mm4_intergenic_Gm25672|Barhl2_chr5_106247903_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25672|Barhl2_chr5_106247903_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Bach1|Gm23994_chr16_87738556_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Bach1|Gm23994_chr16_87738556_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_AC175117.1|Hnf4g_chr3_3201186_F TCGTCGGCAGCGTCTCCTGGCACTTCGGATAGGA 60.0 0.00
mm4_intergenic_AC175117.1|Hnf4g_chr3_3201186_R GTCTCGTGGGCTCGGTCGAAACATTGAGCTGGGAGA 59.3 0.00
mm4_intergenic_4930474N05Rik|Gm24394_chr14_36209749_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_4930474N05Rik|Gm24394_chr14_36209749_R Primer3: not found at this Tm N.d. 0.00
mm3_intron_Gmfb_chr14_46817189_F Primer3: not found at this Tm N.d. 0.00
mm3_intron_Gmfb_chr14_46817189_R Primer3: not found at this Tm N.d. 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

mm3_intergenic_Gm16063|Gm2301_chr5_119626555 CCACCTCCCTAGCCCAAAATTGTAAGAAACCACTTGGAAAACTAATTTCAGCGTCATGGTACAATCTGATTGGACTTTCC
AGAGGGAGGGGGG
AAAACGCTGATAGCCTTCTGCAGA
mm4_exon_Gm16287_chr4_139175852 CTGGGGCTTCGTTCTGTGATCTGCCGAGCCTTTGTTCAGACTCCCAAGAGGGAGGGAGGACCGGTTATTAAAAGAGCAGA
TTCTTCGTTCAATATTTACCCCATACAACATTGCCTTG
mm3_intron_Plag1_chr4_3936878 CCAGAAGGTTCGCCAGTCTTGCCCTCCAGTCTCTCTGGGCAACAGCACCTTTCCACCCCTCCTCTGGAGAGCCCAGCTTA
AAGAAAGGAGGGAGCCACTAACAAAACCCG
mm3_intron_Ccdc30_chr4_119385353 TGCAAATCGCCAATAGTCGCTACAACATTGCAAATTCTCAAATATCAGCTTCCACCTGTATTAGACAGGGCTCTCCAGAG
AGGGGGAGG
GCGGTGAGGGAGGGAAAGACT
mm4_intergenic_Gm24696|Myc_chr15_61984262 TTCTAGGGTCTCTGCAGGCTCCCCAGATCTGGGTTGGCAATTCACTCCTCCCCCTTTCTGGGAAGTCCGGGTTTTCCCCA
ACCCCCCAATTCATGGCATATTCTCGCGTCTAGCGCCTTGATTTTCCCCACCCCA
mm4_intergenic_4930526D03Rik|Oprl1_chr2_181708878 ATTTTCTCTCCCGGCAGCTCTTTTTTCCCGCCCCCCCTCTTAATAGTCCTTTTTGTGTTAAAATATCTTTATTATACTGG
GTAGTCCTTTTCAGTGTTAAAGTCAATGCTGCTGGTTGGGTCTTAG
mm4_intergenic_Gm26580/Kcnq5|Rims1_chr1_22043337 GCAAGAACACTGGGGAGGAAGCTAATAATTCTGAAAAGGGTAGGGGGGGACTCTCCAGAGGAAGTGTGGTTAAAGTTTTT
CTTTGACCATTGAATATATTCCAGGAGAGAAATGCCAGAGGGAGATGGACA
mm4_intergenic_Thumpd2|Slc8a1_chr17_81178734 GGAGGGCCCAAATGCTTAGTTGGAGAGGGGATTGGAAACAGTGTTGAAAGCATTCAAAACTTTGGCCTATGCTTCCTTGG
AAGACCCCCCCCCCCTCTGAAATGTCCCATCACCAGTAGTGTTGGGCATGTGTGGG
mm4_intron_Map4k3_chr17_80646217 CACAAGCTGGGACCCCATACGCAGGACCAGGACGTCTGGGGAAGGGGCAGCAGAGCCACAGGAGTCCCCAGAGGGAGGGC
GG
GAACAGAAAATGTTCCCGATGGCGGCGGTCTGTTTTAT
mm4_intron_Llgl2_chr11_115855262 GGCTCAGCAATGGAGAAGGTAGGGCTGTGGGCACTCCAGAGGAGGGGAGGGCCTGCTGCCAGGGAGGGATGGGAAGGCGG
TCCTTAAAAGAACTGCCACCCCTCC
mm4_intron_Mctp2_chr7_72203189 GCCAATGGTGTAGAGCTGCTGAAAACCTCAAACACATGGAAACACAGAAGCAAACCCTCCAAAGGGGGGGAGGGGCGCTT
AGGTGCCCAGGCATTTGTTTGTTCT
mm4_intergenic_RP23-417D20.1|RP24-129O14.1_chr9_80971705 ATGGGGTGTCCTGGAGAGGAACAAAAAAGAAGGGGGAGAAGAGGGACTCTGTAGAGGAGGGGAGGTTTCTCTGAGAGATG
GTTGACAAACTACTCTACTACTACTACAAAAAAGGGGCATCGGGGTCATGGAAAT
mm4_exon_Pnkd_chr1_74286136 TCTGAAATGCGGGTGGAGAGCTGAATCCAAGGGAAGGAAATTGCTGGAGTCAAGGGATGGACTCTCAGAAGGGGGGGGGG
GGCTAGGGTGGAAAAATATGTAAAATTGGGGGATAGTGGCTTTTCTGTGGAGCA
mm4_intergenic_Atp4b|Grk1_chr8_13403279 ACAACTGTATGTGCTCTGCCATTATCTCAAGAGTTTAACTGAACAAGGATACTCAGAGAGTATACCCTCCCCCCTCTGAA
TAGTCTG
GCCCGGGAACCGAACTCATCCTACTTTGGTGGCAAATTCTCCTCAGCCCCT
mm4_exon_B4galt2/Ccdc24_chr4_117872051 CGGAGGCCTTGGAGTAACTGTCGGAGTTCTTGGCGAATGAGGTCCCTCAAGAGGGGGGGTGGTGCCAGTAGGGAGGGAGG
GTCAGAG
mm4_intron_Rbfox3_chr11_118732586 ATGAAGGAAGGCCACACAGGGTGCCAGCAGCTGGGACTGTGTGACCCCCAAGGTCTGCATCTGCCCTCAGCCTCACTCCC
TCTGTAGAGTCTG
AAAGTCTGGTTTCTGAGCTCCTGCA
mm4_intergenic_Gm25781|Ipo5_chr14_120848705 AAACACTGCCACCCTTTAAAAGGAATGTATTGTTCAGAATTTCTACAAAGCCTAGGCCCACCCCCTCTCTGGCTAGTCTG
AACCGACTTTTACAACCTGCTGTCGCAGAAAGAGCAGCGAGTCA
mm4_intergenic_Frem2|Gm24851_chr3_53691394 GCTATCTGAAGCAGCCACTGACCCCCCCCCCCCTGAAGAGTTCAAATAGTATTATGACCAAGGAAAATATCCCTCCTAGT
GATGGATGCTTGACTTAAGCTCAAGGTTATTTATGGAGTTTGTGGCCTTTGCTG
mm4_intergenic_Gm15387|Bai1_chr15_74483491 AAACCCAGGAAGCATGCAGACCTGCAATGTTTAAGGAAGCCAGCAGACCCAGGCAGATGCCCGCTGGTCAACAGACAGAA
GACAACAGACTCTAAAGGGGGGGGGGGGGGAGACAGTGATGGCGACCACTT
mm4_exon_Prkcd_chr14_30595733 TGATGGCAAAGGGAAGGCAAATTCACAAACAGTTCACAGAGGAAGTACTGTGAGCCCAGCCAGCAGGAGCCGGACTCTCC
AAGGAGGAGGGGG
TGGCAGCAGAAATCAGCCAGTC
mm4_intron_Map1b_chr13_99465616 TCTTTTCCAGCATGTAAACGCATTTTATATCCACCTCTCCTCTGAAGAGTCAGAGAAATGGGGAGAAAGAGAAAAGGGCC
GTCCAC
mm4_intron_Prkca_chr11_108032698 ACGAGGCCATCAACAGTGAGACTCAGTGAGAACAGGAACTTGGTTGGCCGACCTGCCCCCCTCTGTGGAGTCCTCTCCTC
TCAGTCAAGATGGATTGAGCCGAGTAACCATCCAGG
mm4_intergenic_Tcf7|A630014C17Rik_chr11_52283453 GTCGGAACATCAAAGGCACCGCGGGCAGCGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGAGGGGCTAA
CGTCCCAGGCCCCGCCCTCCCCTCCCTGGAGAGACCGGGACAGGCCCACTGGGAATAA
mm4_intergenic_Atp1a3|Grik5_chr7_25008251 GTGCATAACGCTCCCAATGCGAGACTCTGCAGAAGGGGGGTGGGGAGACTTTGGAAGGTCACAGGGATGGAGGA
mm4_intergenic_PVT1_3|Ndufa4_chr6_11485692 ATGCACGGGACCAGAACATTCAAGCACAACACGAGGGATACATGCAGCATGAGAAATGAGAAGATGAAGAAGCCTTCTTG
ACTTTCCAGAGGGGGTGTGG
CAGTAATGGCACCTCATTTCCAGGTT
mm4_intron_Csmd2_chr4_128299765 AGTGCAATTTCTACACCAATTGTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNCTCCTCCCCTCTGGATAGTTTGGGTGGCCCTCACTGTTACTA
mm4_intergenic_Gpr158|Gm13337_chr2_21961447 GAGTCAGCCCAGAAACCACAGTTCACAAACACCAGGTGTGGCCTTTCATATTAACTCAAGACCAAGGAGAGTCTTCATTT
ACATCCTTGCAGACTCTGCAGGGGGAGGGTGGCAGATAGTTTCAGGCAATCCCTTCTGCA
mm4_intergenic_Rhobtb1|Cdk1_chr10_69300423 ACAGAAGAGAGTGGGGAAAGCTGTACTCTACAGAGGGGAGGGGGAGGGGGGCCTTGAGTAGAGCTCTGAACCCTGGGGAT
AGCA
mm4_intergenic_Lce3f|Crct1_chr3_92999996 GGACAGAAATTTGGGAGCTGGATGGGGGAAGCTCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNTCCCAGGAGAGATTGACATTTTGCTTCCAGGTTGTCTCAGG
mm4_intergenic_Camta1|4930589P08Rik_chr4_151349014 CGAAAGGACAGCCACTTCCTAGATCCATAGGAAAAGGAACCGAACTCTCAGGGGGGGGGGGGGAATCCTCCTCAAAGAGC
CAGATTTTCATATAAATAATAGACATGTCGAGGCATAGGATGTCTC
mm4_intron_Epb4.1l1_chr2_156509371 GATGGAGGTATGGCTCAGGCTACAGCTGGGGAGAGAGTGGTCCTCAGAGGAGAGGAGGGGCGTGGACAGGGCGGGTTCTC
CAGAGGAGAGGAGG
GGCGTGGACAGGGCGGGTTCTCCAGAAGAGACAGCCGGTA
mm4_intergenic_Gm7275|Gm7204_chr16_48160037 ACAACAACAGCAAGCCATGTTTTAGTTTAAAGAATCCAGACTCACCAGACAGGGGGTGGGAACACTGTTTATGGTGTGGT
CCAGCTGGGCATATT
mm4_intergenic_Gm15270/Ctgf|Enpp1_chr10_24631982 ACAAGGAAAAGGTGGGCACAAGAATGACGTAAACCACCACCACCCCTCTGGAGAAACCACATCAAAAGCTTCCGAAATAC
AGACGAGGCTCCA
mm4_intergenic_Gm24291|Mon1b_chr8_113551664 GCGTTGTTCTGAACCGGAAGAAGTGAAGACAGCTTCCTCCTACCCCCATCTCAGAGCAGAGCAACTTCCTCTTGACTTTC
CCTCCCCTCCCCTGGAGAATCAGGGAGTAGCAGGTAACACTCCAAGGGAGGAAGGAGAGG
mm4_intron_Aprt_chr8_122575906 CACTTACCAGGGCCACTCTGTTCTCTGGGGTGCCCACCCTCCCTCTGGACACTCCTGGTGGACATTCAGCTGTCCTAGGG
TGTTTCTGAAGCTCAGGCATGGAGCCAGGTAGACAGCAGTTGT
mm4_intron_Slc22a5_chr11_53887775 TGCCATGAAACTGCAAAGGCAAAAAATGAGGGTCTGTGATTCTCCAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGGCTAGCACAGGCAGG
mm4_exon_D630003M21Rik_chr2_158204719 GCAAGCTGGCCAGAGTAGTTCCACCTTCCCTCTGGAGAGCCAGCAGGCCCGGGTCCCTCAGCACCGTCTCCATCAGCTCC
TTGGACTTGCTCA
mm4_intergenic_Gm22597|Gm24514_chr18_55410022 TGTGGCAGATGTGAAGGTCGCCCTTGTAATGGAATAAGTGAGATGGAGAGTGACCCCCCCCCCCCCCCGGAAGAGTCCTG
ATACAACGGCGTTAGATCTAAGATGGCTCCCAAGCAACGGTGAGACAGCAAAA
mm4_intron_Snd1_chr6_28900998 AAAGGCCAAGGAGATGAGGCTGTAATGCACCCTCCAGGGGGGGGGGGGGAGAAACAGAGACCCTTCCCATTCCTATCACA
CACACACCAGGACTT
mm4_intron_Tjp2_chr19_24221091 TCCACTCAGCCTTGCAGAAGTAAGAAGAATTAGCGGTTGGAACGCTGTGAATGCCGGAATCTCTNNNNNNNNNNNNNNNN
NNNNNNNAATGGACAGACTTTCTTGCTAGATCTTGGCTGAGT
mm4_intergenic_Nps|4930544L04Rik_chr7_135388236 TATGTGCAGCAATGGGCCTGCACACATGCACACATGCAAACACACGGAGTCTCTAAAGGGGGGCTGGAGTGATTGAAGCT
GCAATTTAGCAAAAACCTTCGTGTAGAGTCAGTCAACACTGGATGGCTGCAA
mm4_exon_Igf2bp1/Mir3063_chr11_95961323 GGGAAGAGCCGGCTAAGGTACTGTCTCACCAGAGGGGAGGAGGGGTGCCCACAGTTTGACCCCCAAGGGCTAAAGCTCTG
CT
mm4_intron_Pqlc3_chr12_16991776 CCTGTCCCTGTGTCCTGAACTACTTGCTATGGCCAGCCCTGGCCATATTGGGTGTCACTCCTCTGATCAAATCCTTATTT
AGCTTTTAAGACCTTGCCCCCTCTGGAGACTCTGCTCTCAGCTCCCTCCAAGTACACC
mm4_exon_Tbata_chr10_61188831 TCTACCTTGACTCCAGCCCCTGGCCTCCAACAGTACTAAATAAAATCCTGCCCTCCCTCTTGGATGAGGGCAGTTCCACC
CTCCCTCAGGAGTGTCTG
TGTTTAGATGGTTTCTTCTAGAAGAGAAGTGTCCCCTCC
mm4_intergenic_Gm7168|Dact2_chr17_14105711 CAACAGGTCTTCCAGCTCGTCGGACACCCCACAGGGGGCGGGGTATACACCTGCCAGTATCTGCCTTTGCAGCTCAGGTA
TGGTGAC
mm4_intergenic_Tm4sf20|Gm22396/Agfg1_chr1_82833076 TGGCTTCCCTACACTCTGGATTCTTCTTGCTGTCAGTTAACCCCTGCCTCTCTGGAGAGTCAGTCTCAGGTCCCAGAGGA
GCTCGGGGTGAAGGAACAACTGAAGACTAAGAATGGAGATGGGAACACGCCATCA
mm4_exon_Shbg_chr11_69615560 TACCTGGTAAAGCCCCAAGGGAGAGATGGCCCTGAGGGTGGGACCAGAGTCTCCAGAGGGAGGCAGGTCTCAAAAGAGAC
ATAGAAAGGACTTCCATCTTTGGTCCTTGGCTCAAGACC
mm4_intron_Grm2_chr9_106649249 ACACAGGTTCCACAGCAACAGAGCTAGCTAACTCTCCGGGGGGGGGGGGGTGGGGAGGGAGGCAATATATTG
mm4_intergenic_Prdx6|Tnfsf4_chr1_161288471 TCTCTGGCTGTGCTCTCTCTTTGCCCCAGTCGCTGCCTTGTAATCCTGACCCGCCAGAGGGGGCGGGGCCTCGCGGAGGC
ACGCCCCCCACGTCACAGGCTCACTCCCAAT
mm4_intergenic_Gm17118|Cacna2d2_chr9_107460849 CCAGCTAAGTCTGCCAGTCCTCTGCCCCCTCCCCTCTGGAGTGGCTGGCTTCCCCCTGCCCCAGCTCTTCAGTATCATCA
CCATCTTGTCTGCCCCAAGCTC
mm4_exon_Dpy19l4/Gm26663_chr4_11322139 CTCCGAGTATCCAAACCCCGCGGCCGCCCCGGCCAGCTCCCGGCTGCCGCGCCCCCACTTCCTGACCCGCCCCCCTCTGG
TGTCTCCG
CCAACTGGGTCCCTCCAAGGCGCTCCGGGAACCAGAGTGCAGGGAG
mm4_intron_Lasp1_chr11_97808235 GGGCTCACACAAACCCTGATTCTCTGCCACTTCCTCCCCCCAACCCCCCTTTGGAGAGTGGGGTGAGGTTCAGAGGAAAA
AAAAAAAAGCCCAGGGCATTTAACCCTTTGGGTGCTGGACTGCTTTAG
mm4_exon_C230012O17Rik_chr4_24964048 CACATGATGCTTTGTGATCCCCCCGCCCCCCCACTGGAGGGTCATCTCTCCGCAGCTCAATCTACTTTCATGGGGACTGT
GGCCATATTCTCCATCACTGCTCCTGTACATTTAGAAGCAGGCACACAACTGA
mm4_intergenic_Frs2|Gm26311_chr10_117167749 ACAAAAGGCTGTAGGGGACAGATCCCCCCCCCCCCCCCGGGAGTCCGAGCATACGTTACATAAAGGTCATCCTATTCGAG
GTGTGTGTAAGGGA
mm4_intron_Park2_chr17_10963721 CTCCAAGTCTTTGTGAGGCCTATACCAACATCTTTTTAAACTATGTATTTCAACAAAGAATGTTTTACACATACATTTGC
CCCCCCCCCCATGGAGAGTTTG
TACATGGCTTTCATTGCATAATGGT
mm3_intergenic_Gm23558|Galc_chr12_97676887 AGCAGTTTGGGGAAGCTAGAACCATCAAGGATAAAGAGAGGAGGAGTCTCCAGAGGGTGGGGGGGATGTTTGATAATTGG
GGTGAAGGAATTATCCTCTCCCTGATGGGATCCCTCTAGCCTCTC
mm4_intron_Reln_chr5_21900575 CTCTGGCAGGCTGTCTTACCCCCCCCCCCCCCCCCCGAGAGTCTGACCAGATATTGTCCCAAACATTGGCCTCTAGAAGC
CCAAGCAGTAGAAATGAACTTGGTTGTATGATGGAGCTCGCTTTCCC
mm4_intergenic_Gm23980|Gm22810_chr15_24756775 GCAAGGTAGCCATGAAGAACCAACTCCCCCCCCCCCCCCCGAGAGTCTGGATGTTGCACAGTCACACTTACTCTCAGCTC
CTGCATTCAG
mm4_intron_Ttll6_chr11_96146342 AAGCTTTCCCAGAAGCTGCTGGCACGCCACAAATGTCACACTGCTCCTCTGAACAACATGGACTCTTCAGGGGGCGGGCG
G
GAACTGGCCTTCTAGCTGCC
mm4_exon_Nono_chrX_101429638 ACTGGCAGTTCACATAGCCAATCGGGAATCCAAAATTGACCCCCCCCCCCCTCGGGAGGTACCGAGCTCCGTCGGCTCGT
TTCCGGCGGTCGCGCGCTCTTTTCTCGGGACGGTGG
mm4_exon_Col9a1_chr1_24179521 TTGAGAAGGCTGCATCTCGAAGGACTATCCAGAGGGTGGTGGGATCCACAGCCTTACAAGTGG
mm4_intergenic_Gm27043|Gm25020_chr13_17071225 TCATGTTCATGAGTAAACTGAGACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNCTCTCCAAAGTCAG
AGGGATTTTTAGTTGGGAAGAGGGGTTTTATTGGA
mm4_exon_Slmap_chr14_26534421 TTGGTTTCTCGGAGCTCTCGGGCTCTGCTGCTCAGCACATGGGCCACCTCCCCTCAGGAGCGTCCTCGCGAGGGTCGCAT
CGCAGGGACTG
mm4_intron_Nfasc_chr1_132635793 CAGGGCCAGAGCAAACCTCCCCCCCCCCCCCCACTGCATTGTCCGAGACACAGTCTCCTACTCTGAGACAGTAACATATG
ATTTAACATCTTCACTTTGCTACGATGTATCATTTGGCAGAAAGAGAGCTGGCCTTGGT
mm4_intergenic_Pard3|Gm6921_chr8_127518634 TAGCTGCATTTTCCAAGCCGGCCTCCTGAGGGCGGAGCCCCCCCCCCCCCCCCCCAGGAGACTCCACAACATTGGAAACA
TTCTTTCTTTTTTATACCTTTAGAATGCCTTCCAAAGTGAACACTG
mm4_intergenic_Gm24503|Ccrn4l_chr3_50900570 CCCAAACTACCAGGTCAGGGTTTGATACAAAATCAAGCTAGATACAGTGCAAAGTGCCACCTCCGCTCTGGGGAGACCGT
GTGAGCCTCCTATGACTGGGT
mm4_intergenic_Cdkn2b|Gm12609_chr4_89312388 TGCTTCTGGGTGAAACGTCTTCCCGCCCCCCCCTCGGGAGTCCGTCATTCACGGTTGCCAGTGTGCACAGGTAGAATGGG
AGGCGGAGGAA
mm4_intergenic_Gm24444|Mgmt_chr7_136607308 TGCATGCTTGTGAGGTCATCAAATTTAAATTGAGGCTCAGGCCTATAAAGCACATTAATAAAAATACTGTACTCTACAGA
GGGTGGGGGG
AATTTAATATGAATGGCTAAAATGCATGGAATTCTGA
mm4_intron_Gli2_chr1_118887044 AAAGATGAGGCTTGCCCGTTCACAAGACATCTATTGTGCACTGGCAGACATGTGGCAGTCCCCCTACCCCCTGGAGGGTC
CG
AGACAACAGCAGATCTTTGGGTAAGACGGAAGGAGTCCTGTGAGCAC
mm4_intergenic_Trhr2|Gm20681_chr8_122363269 GTCCCAGGGCCAATCTGTACCAACATCAGCAGCCTAGGTCTCTCCAGCAGGGGGGCGGGGAGGGGAAGCATGGACACTGT
CCTCTTGGGCTTG
mm4_intergenic_Cd274|Pdcd1lg2_chr19_29408114 CTCTGACAACCCCTTCACCCCCAACCCCCCCACCCCCGCTCTGAAGAGGCCTTTCTGGGGGAAGGAGATCTGACTTAAAT
TTGACAATTTTGAGTTCAGTAACTTGCCTTTTGGCCAGGAGTCAGAGC
mm4_intergenic_Gm21786|Mpp6_chr6_50045954 TGGCCTCTTTCTGAAGTGCTTGTCTTCCTCCAGCCCTCTGCAGAGTCTGACATGACAGCCCCTTCTTTGAAATGGGATTT
CTGTGCCACTGTTGCTTCTTGAGATGTTTCCCAGTCCTCTCGCTGTTCTTGAGCA
mm4_intergenic_Onecut1/Gm20649|Fam214a_chr9_74907025 CTCTGAGGTCTGTGTGGCTGGAGGAACTAAAGACTCTAGAGAGAGACTTCAACTGCCCTCCCACCCTCGGGTGAGTCCTC
TGATAATATTAACACAGAGCTTCCTGCTACCTCTGAAGTGGGTCCCTGAACTTGG
mm4_intron_Aff1_chr5_103749979 GAGGAGCCCTGCTTGGATACTTGCTGAGCTCTGGGGTTGGGACCCACCCACCCTCTGGAGAGAACAGTCCCTTGGAAGTG
GCCCTGTCCTTA
mm4_intron_Ank3_chr10_69940138 AAAGAGAAGTGGGGCAGGTGCCCCGCCCCCTATGGATACTCCGCCCCCTATAGATGCCCCGCCCCCACGGATGCCTGCTC
CTGTCTCTG
mm4_intergenic_Ppp2r5a|Dtl_chr1_191397144 GGGGTGGAGGAAGGAGTTTGACTGACAACTTCCGCGGGGTCCCACTAGCCTCCGGAGTCTCCGGAGGGGGCCGGGGCGGG
AAAGCATGCCAGTGCCCTC
mm4_intergenic_Gm24979|Cdc5l_chr17_45342185 CAGTCCTGGGGCTTTGTAGGTCTCTTGCATGCCACACGGGCTTTCTCAGTTTTTGTATTGCACAGGTTTTCTAACAGAAG
CTCGGACTCCCGAGATGGGAGGGGGCACAGCAGCCTTTCTCTTTGT
mm4_intron_Lep_chr6_29070427 AAGAGTCATCAGGGCTGCACATGGAGAGGCCCAACTACCCAAATGTGGGTGGAAATGAGAGGAAGCCAGTGGGAAAAGCC
CTTCCTGGTAACCAGACTCAGCAGAGTGGGGGGGGGGGGCACGGCTTTGACCCTAATG
mm4_intron_Ttyh1_chr7_4123683 GCGCCCTATAGCCAAGATTTTTTTTCTCATCAGGCTCTCCAGAATGGGGGTGGATGGGGGTGGGATGTTGAAAAAGGCCC
AGTGGGGGTGAGGCACAGCCCCAGCCCCCTCAGAGCCTAGGAACA
mm4_exon_Ece1_chr4_137963009 AAAAGGGAAGGAGCTGCAGCTAGCTCCCAGCGGAGCCATGTTGCCACCCTCCTCCCAGCCCCTCGGCCTAGGGCCCCTTC
CCCACTCTGGAGAGTATG
CAGCCATCCTAAGCCTAAGATGGATGGAGCCTCACCAC
mm4_exon_Sh3rf2_chr18_42101714 GCCAGCTCTGTGGAAGTCATCAAACAGCTGCCCCAACCACCCCCACTCTGCAGAGCCCTCTACAACTTTGACCTTCGAGA
CAAGGATAAGAGTGAGAACCAGGACTGCCTGACCTTCCTCAAGG
mm4_intergenic_Nlgn2|Tmem256_chr11_69837852 CACACCCCTCGCTTGCACCCGCCTGCCCACCCGCCCTCTGGAGTGTGGGGGGGGATTGAAGACAGTGAAAGGGGTGGGAG
TGGACAGACGGACGGATGGACGTAGCGAACTAGAGCAGGG
mm4_intergenic_A330033J07Rik|Id4/A330033J07Rik_chr13_48240776 CTGAGCTGTTTTGCACACCATAAGGAAGATTATCAACCTCATGAATATAAAGCCAGAACTCTCTCTCCTGAGGGGGGGCG
G
GGGGCGGGAAGCAAAACCCGAACAGGTACGTTTCC
mm4_exon_Zfp324_chr7_12966240 CTATGCCCAGGTCTCTCGCCCGTCATGCCCCGCCCCCACTGCAGATTCCGGCGGCGGCAACCTGGCCGCTGACAAATCCA
CCGCGGGTGAGTGTAGGAATCTTTCCAAGGACCCACCAGG
mm4_exon_Asmt_chrX_170676323 CACCGGAGACAGGAAGTGACGTCACCCCCCCCCCCCGCAGGTGGGTCCGGGGCGCTGGCCCGCATGGCCGCCCGGCTCTA
CCCGGGCAGCGAGGTCACCGTGTTCGAGAC
mm4_intergenic_Zfp385c|Gm11547_chr11_100673393 GAGTAGCTGCCAGGAGCCTCTCCAGCCCCGCTCTGGAGAGCCAGAGAGGGACTCCTTCCCCTGCCTAAAGAGCTAGGCCG
TCG
mm4_intergenic_Cacna1e|Gm9530_chr1_155053516 GGGTGAGGACAGAGAAGGGAAGGGGAGGGTGGGAAGCAGCAGCAGAACAGGAGCTGGCTCCCACTCTCCAGGTGGGGGGT
GG
CAGGAACTGGGAGTGTCTCAAAGCTCAGCCTGG
mm4_intron_Git2_chr5_114766646 TTGGGGCTGGTTTGGAAACAAATAAGAACACAGTTCACTTAACAGCTCTGACACATTTCTCATTCTTTGTGCTGTAAGAA
GAGGAAGTGACACCCCCCCCCCCCCACTGGACAGTAAGAAGGGCAAAGCTGGACTCCACT
mm4_exon_Tmem92_chr11_94777676 CCTCTGCAGCACACACAGTAAACAGAGGGGCAAGGTGCCAAGGCATGGGCACCAGGAACTGTCTTCACTCTCCCCACCCA
CTCTGGACAGTCCA
AGCTCCAAGCTGTCCTCAGCCCCTCAGCCACAGGACAAAA
mm4_intergenic_Manbal|Src_chr2_157396803 TGCCTGTGTTGCTACTGAGGAGGGAAAGGGGCAGCCTGGGTCTCTCCTGAGGGTGGGGGGAACACTGTCCCCAAGAAGTG
GGACAGTGTGGAGAGAAAGGGCAGCTCTG
mm4_intergenic_Il13|Rps2-ps4_chr11_53635711 ACACCCGCACATAGCCTTTTCCCTACTCTTTCCTCATACTGAGCCACCCACCCCCTGCAGAGGCCGCAGCATTTGCCTAT
TGGGTAGTGATGTCAAGATGCACCCTCACC
mm3_exon_Sympk_chr7_19045830 TGAGAGAGGGAGCAGGACAAGAGTAAGCTTGCACAACAGCCTGAGCATCACCCCCCCTCTGCAGAGTCGTGCCTACCTGG
GCATGTCGACACTTGGAGA
mm4_intron_Pitx1_chr13_55833965 GGTTCACTGAAGCAGGTCGAGTTCTATTCCACTACCCTTCCCACCTGTGCAGAGTCCGAAATATTTCTCTGTCCTTCAAG
AAACCGGGAGACGGTGATCTGGAGATGCCTGCATTGTTGTGGG
mm4_intron_Parva_chr7_112557588 CCTAGCTCTCACACCCTCCTCCTCCCTCCCTCCCAACCTCTGCAGAGACCGGAGTCGGTCACCAGCAGTCTAG
mm4_intron_Clstn2_chr9_97796195 CATGTGACTGAAAGGAGGCCTACTGAAATGGAGTCTCTAGAGGCGGGGAGGTATAGCTCTGTGCTTTTTAATGTGTACTT
TATAGTACTTTCCATGTTTCCTATAGCCAGTGC
mm4_intergenic_Klf2|Eps15l1_chr8_72333059 GAAAGGACCCTGCAGCTCTTGCCCATCTGTTGTTGCTTCTGTGGAAGCTGATGTTTATGGCTGAACAATTGCTACAAACT
GACTTTCCTGAGGTGGGGTGG
TGGTGGGGGGACTCCTATGGTGAGAGCCCT
mm4_intergenic_Gm26556|Ppp1r9a_chr6_4971080 AATTCTGACTGGGTGGAGGCTGGTCTGGAACCCCCTCACCTCAGGAGACTCCGATTGTTTGGACTGGAAATTGTACTTGC
ACTTGGATTGAGGGATGGCA
mm4_intergenic_AC175117.1|Hnf4g_chr3_3201186 TCCTGGCACTTCGGATAGGAAACATACTCCAAGAACCACAGTCAGCCCTCCCACCCCTGGAGAGTCAGAACTGAGCCTGG
GTCTCCCAGCTCAATGTTTCGA

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_mN1FkRligAThyqKv3eg3.txt --name MY_EXPERIMENT

Version 5.01 - Documentation  - Contact us - Downloads/local installation - Citation - License
CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.