CRISPOR

DNA break repair outcome predictions

Guide: TTGGCCAGAGAGGCTGTCCG CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TCTGAGCTACTAGGTTGCCCTTGGCCAGAGAGGCTGTCCGCGGAGGGGACTGGGGGTCCCGGGCCAAGCATGGCCGCTCC`	(Wild-type sequence)
365	`TAGGTTGCCCTTGGCCAGAGAGG-------------GGACTGGGGGTCCCGGGCCAAGCA`	`13 bp deleted → frameshift`
362	`TAGGTTGCCCTTGGCCAGAGAGGCTGTCCG--GAGGGGACTGGGGGTCCCGGGCCAAGCA`	`2 bp deleted → frameshift`
257	`TAGGTTGCCCTTGGCCAGAGAGGCTGTCC--------------------CGGGCCAAGCA`	`20 bp deleted → frameshift`
242	`TAGGTTGCCCTTGGCCAGAGAGG----------AGGGGACTGGGGGTCCCGGGCCAAGCA`	`10 bp deleted → frameshift`
235	`TAGGTTGCCCTTGGCCAGAG---------------GGGACTGGGGGTCCCGGGCCAAGCA`	`15 bp deleted → no frameshift`
224	`TAGGTTGCCCTTGGCCAGAGAGGCTG----------------GGGGTCCCGGGCCAAGCA`	`16 bp deleted → frameshift`
210	`TAGGTTGCCCTTGGCCAGAGAGGCTGTCCG---------------------GGCCAAGCA`	`21 bp deleted → no frameshift`
198	`TAGGTTGCCCTTGGCCAGAGAGG--------------GACTGGGGGTCCCGGGCCAAGCA`	`14 bp deleted → frameshift`
188	`TAGGTTGCCCTTGGCCAGAGAGG---------------ACTGGGGGTCCCGGGCCAAGCA`	`15 bp deleted → no frameshift`
154	`TAGGTTGCCCTTGG-------------------AGGGGACTGGGGGTCCCGGGCCAAGCA`	`19 bp deleted → frameshift`
147	`TAGGTTGCCCTTGGCCAGAGAGG--------------------GGGTCCCGGGCCAAGCA`	`20 bp deleted → frameshift`
140	`TAGGTTGCCCTTGGCCAGAGAGG---------------------GGTCCCGGGCCAAGCA`	`21 bp deleted → no frameshift`
133	`TAGGTTGCCCTTGGCCAGAGAGGC------------------------------CAAGCA`	`30 bp deleted → no frameshift`
133	`TAGGTTGCCCTTGGCCAGAGAGG----------------------GTCCCGGGCCAAGCA`	`22 bp deleted → frameshift`
133	`TAGGTTGCCCTTGG----------------------GGACTGGGGGTCCCGGGCCAAGCA`	`22 bp deleted → frameshift`
128	`TAGGTTGCCCTTGGCCAG-----------------GGGACTGGGGGTCCCGGGCCAAGCA`	`17 bp deleted → frameshift`
127	`TAGGTTGCCCTTGGCCA---------------------------------------AGCA`	`39 bp deleted → no frameshift`
126	`TAGGTTGCCCTTGGCCAGAGAGG-----------------------TCCCGGGCCAAGCA`	`23 bp deleted → frameshift`
126	`TAGGTTGCCCTTGG-----------------------GACTGGGGGTCCCGGGCCAAGCA`	`23 bp deleted → frameshift`
122	`TAGGTTGCCCTTGGCCAGAGA------------------CTGGGGGTCCCGGGCCAAGCA`	`18 bp deleted → no frameshift`
120	`TAGGTTGCCCTTGG------------------------ACTGGGGGTCCCGGGCCAAGCA`	`24 bp deleted → no frameshift`
117	`TAGGTTGCCCTTGG-----------------------------GGGTCCCGGGCCAAGCA`	`29 bp deleted → frameshift`
110	`TAGGTTGCCCTTGGCCAGA--------------------CTGGGGGTCCCGGGCCAAGCA`	`20 bp deleted → frameshift`
109	`TAGGTTGCCCTTGGCCAGAGAGGCTGTCC--------------------------AAGCA`	`26 bp deleted → frameshift`
101	`TAGG--------------------------------GGACTGGGGGTCCCGGGCCAAGCA`	`32 bp deleted → frameshift`
94	`TAGGTTGCCCTTGGCCAGAGAGG-----------------------------GCCAAGCA`	`29 bp deleted → frameshift`
94	`TAGG-----------------------------AGGGGACTGGGGGTCCCGGGCCAAGCA`	`29 bp deleted → frameshift`
89	`TAGGTTGCCCTTGG------------------------------GGTCCCGGGCCAAGCA`	`30 bp deleted → no frameshift`
84	`TAGGTTGCCCTTGG-------------------------------GTCCCGGGCCAAGCA`	`31 bp deleted → frameshift`
81	`TAGGTTGCCC----------------------------------------GGGCCAAGCA`	`40 bp deleted → frameshift`
80	`TAGGTTGCCCTTGG--------------------------------TCCCGGGCCAAGCA`	`32 bp deleted → frameshift`
76	`TAGGTTGCCCTTGGCC---------------------------------CGGGCCAAGCA`	`33 bp deleted → no frameshift`
76	`TAGG---------------------------------GACTGGGGGTCCCGGGCCAAGCA`	`33 bp deleted → no frameshift`
73	`TAGGTTGCCCTTGGCC----------------------------------GGGCCAAGCA`	`34 bp deleted → frameshift`
73	`TAGG----------------------------------ACTGGGGGTCCCGGGCCAAGCA`	`34 bp deleted → frameshift`
69	`TAGGTTGCCCTTGGCCAGAGAGGC-----------------------------------A`	`35 bp deleted → frameshift`
66	`TAGGTTGCCCT------------------------------GGGGGTCCCGGGCCAAGCA`	`30 bp deleted → no frameshift`
61	`TAGGT------------------------------------------CCCGGGCCAAGCA`	`42 bp deleted → no frameshift`
60	`TAGGTTGCCCTTGG--------------------------------------GCCAAGCA`	`38 bp deleted → frameshift`
60	`TAGGTTGCC----------------------------------------------AAGCA`	`46 bp deleted → frameshift`
56	`TAGGTTGCCC---------------------------------------CGGGCCAAGCA`	`39 bp deleted → no frameshift`
56	`TAGG---------------------------------------GGGTCCCGGGCCAAGCA`	`39 bp deleted → no frameshift`
54	`TAGG----------------------------------------GGTCCCGGGCCAAGCA`	`40 bp deleted → frameshift`
52	`TAGGTTG-----------------------------------GGGGTCCCGGGCCAAGCA`	`35 bp deleted → frameshift`
51	`TAGGTTGCC-----------------------------------------GGGCCAAGCA`	`41 bp deleted → frameshift`
51	`TAGG-----------------------------------------GTCCCGGGCCAAGCA`	`41 bp deleted → frameshift`
49	`TAGGTTGCCCTTGGCCAGAGAG------------------------------------CA`	`36 bp deleted → no frameshift`
45	`TAGGTTGCCCTTGGCCAGAG--------------------------------------CA`	`38 bp deleted → frameshift`
44	`TAGGTTGCCCTTGGC--------------------------------------------A`	`44 bp deleted → frameshift`
42	`TAGGTTGCCC---------------------------------------------AAGCA`	`45 bp deleted → no frameshift`
40	`TAGGTTGCCCTTGGCCAG----------------------------------------CA`	`40 bp deleted → frameshift`
36	`TAGG------------------------------------------------GCCAAGCA`	`48 bp deleted → no frameshift`
34	`TAGGTTGCCCTTGGCCA-------------------------------------------`	`43 bp deleted → frameshift`
34	`TAGG-------------------------------------------------CCAAGCA`	`49 bp deleted → frameshift`
31	`TAGGTTGC---------------------------------------------------A`	`51 bp deleted → no frameshift`
19	`TAG-------------------------------------------------------CA`	`55 bp deleted → frameshift`

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