CRISPOR

DNA break repair outcome predictions

Guide: GACTAGCAGGCAGTGCTGCA GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GGAGGAGGAGCCGGTCGATGGACTAGCAGGCAGTGCTGCAGGGCTGGGCGCCGAGCCACGGTCTGCTGGAGCGGCCATGC`	(Wild-type sequence)
469	`CCGGTCGATGGACTAGCAGGCAGTGCTG--------GGCGCCGAGCCACGGTCTGCTGGA`	`8 bp deleted → frameshift`
313	`CCGGTCGATGGACTAGCAGGC-------------TGGGCGCCGAGCCACGGTCTGCTGGA`	`13 bp deleted → frameshift`
311	`CCGGTCGATGGACTAGCAGGCAGTGCTGC-----TGGGCGCCGAGCCACGGTCTGCTGGA`	`5 bp deleted → frameshift`
244	`CCGGTCGATGGACTAGCAGGC------------------GCCGAGCCACGGTCTGCTGGA`	`18 bp deleted → no frameshift`
242	`CCGGTCGATGGACTAGCAGGCAGTGCTGC----------GCCGAGCCACGGTCTGCTGGA`	`10 bp deleted → frameshift`
219	`CCGGTCGATGGACTAGCAGGCAGTGCTGC------------CGAGCCACGGTCTGCTGGA`	`12 bp deleted → no frameshift`
219	`CCGGTCGATGGACTAGCAGG------------GCTGGGCGCCGAGCCACGGTCTGCTGGA`	`12 bp deleted → no frameshift`
208	`CCGGTCGATGGACTAGCAGGCAGTGC-------------GCCGAGCCACGGTCTGCTGGA`	`13 bp deleted → frameshift`
188	`CCGGTCGATGGACTAGCAGGCAGTGC---------------CGAGCCACGGTCTGCTGGA`	`15 bp deleted → no frameshift`
181	`CCGGTCGATGGACTAGCAGGCAGTGCTGC---------------------------TGGA`	`27 bp deleted → no frameshift`
178	`CCGGTCGATGGACTAGCAGGCAGTGCTG------------------------------GA`	`30 bp deleted → no frameshift`
173	`CCGGTCGATGGACTAGCAGGCAGTG-----------GGCGCCGAGCCACGGTCTGCTGGA`	`11 bp deleted → frameshift`
170	`CCGGTCGATGGACTAGCAGGCAGTGCTGC-----------------CACGGTCTGCTGGA`	`17 bp deleted → frameshift`
170	`CCGGTCGATGGACTAGCAGG-----------------GCGCCGAGCCACGGTCTGCTGGA`	`17 bp deleted → frameshift`
170	`CCGGTCGATGGACTAGC-----------------TGGGCGCCGAGCCACGGTCTGCTGGA`	`17 bp deleted → frameshift`
147	`CCGGTCGATGGACTAGCAGGCAGTGC--------------------CACGGTCTGCTGGA`	`20 bp deleted → frameshift`
147	`CCGGTCGATGGACTAGCAGGC--------------------CGAGCCACGGTCTGCTGGA`	`20 bp deleted → frameshift`
140	`CCGGTCGATGG---------------------GCTGGGCGCCGAGCCACGGTCTGCTGGA`	`21 bp deleted → no frameshift`
136	`CCGGTCGATGG--------------------------GCGCCGAGCCACGGTCTGCTGGA`	`26 bp deleted → frameshift`
133	`CCGGTCGATGGACTAGC----------------------GCCGAGCCACGGTCTGCTGGA`	`22 bp deleted → frameshift`
133	`CCGGTCGATGG----------------------CTGGGCGCCGAGCCACGGTCTGCTGGA`	`22 bp deleted → frameshift`
122	`CCGGTCGATGGACTAGCAGGCAGTGCTGCA------------------CGGTCTGCTGGA`	`18 bp deleted → no frameshift`
120	`CCGGTCGATGGACTAGC------------------------CGAGCCACGGTCTGCTGGA`	`24 bp deleted → no frameshift`
117	`CCGGTCGATGGACTAGC-----------------------------CACGGTCTGCTGGA`	`29 bp deleted → frameshift`
114	`CCGGTCGATGGACTAGCAGGC-------------------------CACGGTCTGCTGGA`	`25 bp deleted → frameshift`
105	`CCGGTCGATGGACT---------------------GGGCGCCGAGCCACGGTCTGCTGGA`	`21 bp deleted → no frameshift`
103	`CCGGTCGATGG---------------------------CGCCGAGCCACGGTCTGCTGGA`	`27 bp deleted → no frameshift`
99	`CCGGTCGATGGACTAGCAGGCAG----------------------CCACGGTCTGCTGGA`	`22 bp deleted → frameshift`
98	`CCGG----------------------------GCTGGGCGCCGAGCCACGGTCTGCTGGA`	`28 bp deleted → frameshift`
94	`CCGG-----------------------------CTGGGCGCCGAGCCACGGTCTGCTGGA`	`29 bp deleted → frameshift`
85	`CCGGTC-----------------------------------------------TGCTGGA`	`47 bp deleted → frameshift`
84	`CCGGTCGATGGACTAGCAGG-------------------------------TCTGCTGGA`	`31 bp deleted → frameshift`
82	`CCGGTCGA------------------------------------GCCACGGTCTGCTGGA`	`36 bp deleted → no frameshift`
81	`CCGGTCGATGGACTAGCAGGCA--------------------------CGGTCTGCTGGA`	`26 bp deleted → frameshift`
81	`CCGGTCGATGGACTAGCAG--------------------------CCACGGTCTGCTGGA`	`26 bp deleted → frameshift`
81	`CCG----------------------------------------AGCCACGGTCTGCTGGA`	`40 bp deleted → frameshift`
76	`CCGGTCG---------------------------------CCGAGCCACGGTCTGCTGGA`	`33 bp deleted → no frameshift`
76	`CCGG---------------------------------GCGCCGAGCCACGGTCTGCTGGA`	`33 bp deleted → no frameshift`
74	`CCGGTCGATGGACTAGCAGGCAGT----------------------------CTGCTGGA`	`28 bp deleted → frameshift`
73	`CCGG----------------------------------CGCCGAGCCACGGTCTGCTGGA`	`34 bp deleted → frameshift`
69	`CCGGTCGATGGACTAGCAGGC-----------------------------------TGGA`	`35 bp deleted → frameshift`
66	`CCGGTCGATGGACTAGCA------------------------------CGGTCTGCTGGA`	`30 bp deleted → no frameshift`
62	`CCG-------------------------------------CCGAGCCACGGTCTGCTGGA`	`37 bp deleted → frameshift`
60	`CCGGTCGATGGA--------------------------------GCCACGGTCTGCTGGA`	`32 bp deleted → frameshift`
57	`CCGGTCGATGGACTAGCAGGCAGTG---------------------------------GA`	`33 bp deleted → no frameshift`
56	`CCGGTCGATGGACTAGCAGG---------------------------------------A`	`39 bp deleted → no frameshift`
56	`CCGGTCGATGGACTAGC---------------------------------------TGGA`	`39 bp deleted → no frameshift`
54	`CCGGTCGATGGA------------------------------------------------`	`48 bp deleted → no frameshift`
54	`CCGGTCGATGG----------------------------------------TCTGCTGGA`	`40 bp deleted → frameshift`
49	`CCGGTCGATGGAC------------------------------------GGTCTGCTGGA`	`36 bp deleted → no frameshift`
46	`CCGGTCG-------------------------------------------GTCTGCTGGA`	`43 bp deleted → frameshift`
42	`CC---------------------------------------------ACGGTCTGCTGGA`	`45 bp deleted → no frameshift`
40	`CCGGTCGATGGACT----------------------------------------GCTGGA`	`40 bp deleted → frameshift`
34	`CCGGTCGATGGACT-------------------------------------------GGA`	`43 bp deleted → frameshift`
31	`CCGGTCGATG---------------------------------------------CTGGA`	`45 bp deleted → no frameshift`
25	`CCGG-------------------------------------------------------A`	`55 bp deleted → frameshift`
22	`CCGGTCGA----------------------------------------------------`	`52 bp deleted → frameshift`

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