CRISPOR

DNA break repair outcome predictions

Guide: GTGCTGGTACTCGGCGTTCC CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GCAAGGTGGCTACCTTTGCTGTGCTGGTACTCGGCGTTCCCGGTGGCGCAGTCTGGGGTGCAGCTCACCTCGATTTCTTC`	(Wild-type sequence)
439	`TACCTTTGCTGTGCTGGTACTCGGCG------------CAGTCTGGGGTGCAGCTCACCT`	`12 bp deleted → no frameshift`
382	`TACCTTTGCTGTGCTGGTACTCGG---------TGGCGCAGTCTGGGGTGCAGCTCACCT`	`9 bp deleted → no frameshift`
296	`TACCTTTGCTGTGCTGGTACTCGGCG------GTGGCGCAGTCTGGGGTGCAGCTCACCT`	`6 bp deleted → no frameshift`
224	`TACCTTTGCTGTGCTGGT----------------GGCGCAGTCTGGGGTGCAGCTCACCT`	`16 bp deleted → frameshift`
211	`TACCTTTGCTGTGCTGGTACTCGGCGT-------GGCGCAGTCTGGGGTGCAGCTCACCT`	`7 bp deleted → frameshift`
198	`TACCTTTGCTGTGCTGGTACTCGGC--------------AGTCTGGGGTGCAGCTCACCT`	`14 bp deleted → frameshift`
193	`TACCTTTGCTGTGCTGG-------------------CGCAGTCTGGGGTGCAGCTCACCT`	`19 bp deleted → frameshift`
188	`TACCTTTGCTGTGCTGGTACTCG---------------CAGTCTGGGGTGCAGCTCACCT`	`15 bp deleted → no frameshift`
166	`TACCTTTGCTGTG----------------------GCGCAGTCTGGGGTGCAGCTCACCT`	`22 bp deleted → frameshift`
164	`TACCTTTGCTGTGCTGG-----------------------------GGTGCAGCTCACCT`	`29 bp deleted → frameshift`
149	`TACCTTTGCTGTGCTGGTACTCGGCGTTC--------------TGGGGTGCAGCTCACCT`	`14 bp deleted → frameshift`
141	`TACCTTTGCTGTGCTGGTACTCGGCGT---------------CTGGGGTGCAGCTCACCT`	`15 bp deleted → no frameshift`
133	`TACCTTTGCTGTGCTGGTACTCGG----------------------GGTGCAGCTCACCT`	`22 bp deleted → frameshift`
126	`TACCTTTGCTGTGCTGGTACTCGG-----------------------GTGCAGCTCACCT`	`23 bp deleted → frameshift`
126	`TACCTTTGCTGTGC-----------------------GCAGTCTGGGGTGCAGCTCACCT`	`23 bp deleted → frameshift`
120	`TACCTTTGCTGTGCTGGTACTCGG------------------------TGCAGCTCACCT`	`24 bp deleted → no frameshift`
114	`TACCTTTGCTGTGC-------------------------AGTCTGGGGTGCAGCTCACCT`	`25 bp deleted → frameshift`
109	`TACCTTTGCTGTGC-------------------------------------AGCTCACCT`	`37 bp deleted → frameshift`
109	`TACCTTTGCTGTGCTGGTACTCGGC--------------------------AGCTCACCT`	`26 bp deleted → frameshift`
106	`TACCTTTGCTGTGCTGGT-------------------------------GCAGCTCACCT`	`31 bp deleted → frameshift`
105	`TACCTTTGCTGTGCTGGTACTC---------------------TGGGGTGCAGCTCACCT`	`21 bp deleted → no frameshift`
99	`TACCTTTGCTGTGCTGGTACTCGGCGT----------------------GCAGCTCACCT`	`22 bp deleted → frameshift`
98	`TACCTTTGC----------------------------GCAGTCTGGGGTGCAGCTCACCT`	`28 bp deleted → frameshift`
95	`TACCTTTGCTGTGCTGGTACT-----------------------GGGGTGCAGCTCACCT`	`23 bp deleted → frameshift`
94	`TACCTTTGCTGTGCTGGTACTCGGCGTTCC-----------------------------T`	`29 bp deleted → frameshift`
94	`TACCTTTGCTGTGCTGGTACTCGGC-----------------------------TCACCT`	`29 bp deleted → frameshift`
91	`TACCTTTGCTGTGCTGGTACTC----------------------------------ACCT`	`34 bp deleted → frameshift`
91	`TACCTTTGCTG----------------------------------GGGTGCAGCTCACCT`	`34 bp deleted → frameshift`
90	`TACCTTTGCTGTGCTGGT------------------------CTGGGGTGCAGCTCACCT`	`24 bp deleted → no frameshift`
90	`TACCTTTGCTG------------------------GCGCAGTCTGGGGTGCAGCTCACCT`	`24 bp deleted → no frameshift`
89	`TACCTTTGCTGTGCTGG------------------------------GTGCAGCTCACCT`	`30 bp deleted → no frameshift`
89	`TACCTTTGC------------------------------AGTCTGGGGTGCAGCTCACCT`	`30 bp deleted → no frameshift`
77	`TACCTTTGCTGTGCTGGTACTCGGCGTTC---------------------------ACCT`	`27 bp deleted → no frameshift`
77	`TACCTTTG---------------------------GCGCAGTCTGGGGTGCAGCTCACCT`	`27 bp deleted → no frameshift`
67	`TACCTTTGCTGTGCT----------------------------------------CACCT`	`40 bp deleted → frameshift`
66	`TACCTTTGCTGT------------------------------CTGGGGTGCAGCTCACCT`	`30 bp deleted → no frameshift`
61	`TACCTTTGC------------------------------------------AGCTCACCT`	`42 bp deleted → no frameshift`
60	`TACCTTTGCTGTG--------------------------------GGGTGCAGCTCACCT`	`32 bp deleted → frameshift`
54	`TACCTTTGCTGTGCTG----------------------------------CAGCTCACCT`	`34 bp deleted → frameshift`
52	`TACCTTTGCT---------------------------------------------CACCT`	`45 bp deleted → no frameshift`
47	`TACCTTTG-------------------------------------GGGTGCAGCTCACCT`	`37 bp deleted → frameshift`
45	`TACCTTTGCTGTGCTGGTAC--------------------------------------CT`	`38 bp deleted → frameshift`
42	`TACCTTTGCTGTGCTGGTACT---------------------------------------`	`39 bp deleted → no frameshift`
42	`TACCTTTGCTG---------------------------------------CAGCTCACCT`	`39 bp deleted → no frameshift`
42	`TACCT---------------------------------------GGGGTGCAGCTCACCT`	`39 bp deleted → no frameshift`
38	`TACCT-------------------------------------------------------`	`55 bp deleted → frameshift`
31	`TACCTTTGCTGTGCT---------------------------------------------`	`45 bp deleted → no frameshift`
24	`TACCTTTGCT--------------------------------------------------`	`50 bp deleted → frameshift`
24	`TACCT--------------------------------------------------CACCT`	`50 bp deleted → frameshift`

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